CN113528323A - Nucleic acid extraction device and detection system - Google Patents
Nucleic acid extraction device and detection system Download PDFInfo
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- CN113528323A CN113528323A CN202110888351.0A CN202110888351A CN113528323A CN 113528323 A CN113528323 A CN 113528323A CN 202110888351 A CN202110888351 A CN 202110888351A CN 113528323 A CN113528323 A CN 113528323A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 72
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 72
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 72
- 238000000605 extraction Methods 0.000 title claims abstract description 63
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 230000003321 amplification Effects 0.000 claims abstract description 20
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 20
- 230000007246 mechanism Effects 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 10
- 239000012085 test solution Substances 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 239000011324 bead Substances 0.000 claims description 7
- 238000005382 thermal cycling Methods 0.000 claims description 7
- 238000002955 isolation Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 19
- 238000012360 testing method Methods 0.000 abstract description 15
- 230000008569 process Effects 0.000 abstract description 13
- 238000011109 contamination Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 description 13
- 230000009471 action Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本公开涉及检测设备技术领域，尤其涉及一种核酸提取装置及检测系统。该核酸提取装置包括滑轨组件和机械臂机构，机械臂机构与滑轨组件滑动连接，机械臂机构包括基座、用于吸取试液的移液泵机械臂组件和用于提取核酸的提取机械臂组件，基座与滑轨组件滑动连接，移液泵机械臂组件和提取机械臂组件均沿垂直于基座的滑动方向与基座滑动连接。通过设置滑轨组件、基座、设置在基座上的移液泵机械臂组件和提取机械臂组件，实现了核酸的提取和扩增过程的自动化，避免了核酸提取和扩增过程中的人工介入，从而避免了人工介入对检测结果的影响，降低了测试过程中样本及试剂被污染的概率，有效提高了加样稳定性、检测结果的一致性以及检测结果的准确性。
The present disclosure relates to the technical field of detection equipment, and in particular, to a nucleic acid extraction device and a detection system. The nucleic acid extraction device includes a slide rail assembly and a robotic arm mechanism, the robotic arm mechanism is slidably connected to the slide rail assembly, and the robotic arm mechanism includes a base, a pipette pump robotic arm assembly for absorbing test solution, and an extraction machine for extracting nucleic acid The arm assembly, the base is slidably connected with the slide rail assembly, and the pipetting pump mechanical arm assembly and the extraction mechanical arm assembly are all slidably connected with the base along a sliding direction perpendicular to the base. By arranging the slide rail assembly, the base, the pipette pump mechanical arm assembly and the extraction mechanical arm assembly arranged on the base, the automation of the nucleic acid extraction and amplification process is realized, and the manual operation in the nucleic acid extraction and amplification process is avoided. Intervention, thereby avoiding the influence of manual intervention on the test results, reducing the probability of contamination of samples and reagents during the test process, and effectively improving the stability of sample addition, the consistency of test results, and the accuracy of test results.
Description
Technical Field
The disclosure relates to the technical field of detection equipment, in particular to a nucleic acid extraction device and a detection system.
Background
Most of the existing nucleic acid detection devices can only finish the extraction or amplification of nucleic acid independently, when the nucleic acid is detected, a plurality of devices are required to work simultaneously, and nucleic acid needs to be sucked out manually and transferred to an amplification instrument to finish the nucleic acid amplification process.
In the process, the equipment is various, manual intervention is more, the sample adding stability is poor, the result consistency and the result accuracy are greatly influenced by testers, and samples and reagents are easily polluted.
Disclosure of Invention
In order to solve the above technical problem or at least partially solve the above technical problem, the present disclosure provides a nucleic acid extraction device and a detection system.
The present disclosure provides a nucleic acid extraction device, comprising a slide rail assembly and a mechanical arm mechanism, wherein the mechanical arm mechanism is connected with the slide rail assembly in a sliding manner,
the mechanical arm mechanism comprises a base, a pipetting pump mechanical arm assembly for sucking a test solution and an extraction mechanical arm assembly for extracting nucleic acid, wherein the base is connected with the sliding rail assembly in a sliding manner, and the pipetting pump mechanical arm assembly and the extraction mechanical arm assembly are connected with the base in a sliding manner along a sliding direction perpendicular to the base.
Optionally, the extraction robot assembly comprises a magnetic bar robot assembly, and the magnetic bar robot assembly comprises a magnetic bar for attracting magnetic beads and a first driving assembly for driving the magnetic bar to move along a sliding direction perpendicular to the base;
the magnetic bar mechanical arm assembly further comprises a first connecting arm, one end of the first connecting arm is connected with the first driving assembly, and the end part of the other end of the first connecting arm is provided with the magnetic bar.
Optionally, the magnetic rod mechanical arm assembly further comprises a first guide assembly for limiting the movement direction of the magnetic rod, the first guide assembly is disposed on the base, and the first connecting arm is connected with the first guide assembly.
Optionally, the mechanical arm assembly comprises a magnetic rod sleeve mechanical arm assembly, the magnetic rod sleeve mechanical arm assembly comprises a magnetic rod sleeve switching sleeve matched with the magnetic rod sleeve and a second driving assembly used for driving the magnetic rod sleeve switching sleeve to move along the direction perpendicular to the sliding direction of the base, and the magnetic rod sleeve switching sleeve is arranged on the outer side of the magnetic rod.
Optionally, the bar magnet sleeve mechanical arm assembly further comprises a second connecting arm, one end of the second connecting arm is connected with the second driving assembly, and the end of the other end of the second connecting arm is provided with the bar magnet sleeve adapter sleeve.
Optionally, the magnetic rod mechanical arm assembly further comprises a second guide assembly for limiting the movement direction of the magnetic rod sleeve adapter sleeve, the second guide assembly is arranged on the base, and the second connecting arm is connected with the second guide assembly.
Optionally, a side of the second connecting arm facing the base is provided with a reaction cup hot cover.
Optionally, the pipetting pump mechanical arm assembly comprises a pipetting pump assembly for sucking the test solution and a third driving assembly for driving the pipetting pump assembly to move along the sliding direction perpendicular to the base;
the liquid-transfering pump subassembly is including being used for taking the adapter of pipettor suction head.
The present disclosure also provides a detection system comprising the above nucleic acid extraction device.
Optionally, the kit further comprises an electronic control system, a reagent kit component and a thermal cycling component, wherein the electronic control system is electrically connected with the nucleic acid extraction device, and the thermal cycling component is used for realizing amplification reaction and detection of nucleic acid.
The utility model provides an including sliding connection's mechanical arm mechanism and slide rail set spare among the nucleic acid extraction element, mechanical arm mechanism passes through base and slide rail set spare sliding connection, is provided with on the base and moves liquid-transfering pump mechanical arm subassembly and draws mechanical arm subassembly, moves liquid-transfering pump mechanical arm subassembly and draws mechanical arm subassembly and all along the slip direction and the base sliding connection of perpendicular to base, moves liquid-transfering pump mechanical arm subassembly promptly and draws mechanical arm subassembly along the plane direction motion of perpendicular to base place. When nucleic acid detection is carried out, the mechanical arm assembly of the pipetting pump moves along the direction vertical to the plane of the base so as to finish the suction and addition actions of a nucleic acid sample, a reagent and sealing oil, then the base slides relative to the sliding rail assembly so as to adjust the relative position of the mechanical arm assembly for extraction and the nucleic acid sample, the mechanical arm assembly for extraction and the sample are arranged oppositely, and then the mechanical arm assembly for extraction is controlled to move along the direction vertical to the plane of the base so as to realize the extraction of nucleic acid. When the extracted nucleic acid is required to be transferred into the amplification instrument, after the extraction mechanical arm assembly finishes extracting the nucleic acid, the base is controlled to move along the sliding rail assembly, so that the mechanical arm mechanism moves to the amplification instrument, then the extraction mechanical arm assembly moves towards the direction close to the base, the extracted nucleic acid and the reagent are placed into the amplification instrument, and the amplification and detection processes of the nucleic acid are finished.
Through setting up slide rail set spare, base, setting up the liquid-transfering pump mechanical arm subassembly on the base and drawing mechanical arm subassembly, realized the automation of drawing and the amplification process of nucleic acid, avoided the nucleic acid to draw and the artifical intervention of amplification in-process to avoid artifical intervention to the influence of testing result, effectively improved application of sample stability, the uniformity of testing result and the accuracy of testing result.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.
In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present disclosure, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive exercise.
FIG. 1 is a schematic structural diagram of a detection system according to an embodiment of the present disclosure;
FIG. 2 is a schematic structural diagram of a nucleic acid extraction apparatus according to an embodiment of the present disclosure;
FIG. 3 is a rear view of the nucleic acid extraction apparatus according to the embodiment of the present disclosure.
Wherein, 1-a slide rail component; 2-a base; 3-a pipetting pump mechanical arm assembly; 31-a pipetting pump assembly; 311-adapter; 32-a third drive assembly; 33-a third connecting arm; 34-a third guide assembly; 4-a magnetic bar mechanical arm assembly; 41-magnetic bar; 42-a first drive assembly; 43-a first connecting arm; 44-a first guide assembly; 5-the magnetic bar is sleeved with the mechanical arm assembly; 51-a magnetic rod sleeve adapter sleeve; 52-a second drive assembly; 53-a second connecting arm; 54-a second guide assembly; 55-hot lid of reaction cup; 6-an electronic control system; 7-a kit component; 8-thermal cycling assembly.
Detailed Description
In order that the above objects, features and advantages of the present disclosure may be more clearly understood, aspects of the present disclosure will be further described below. It should be noted that the embodiments and features of the embodiments of the present disclosure may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present disclosure, but the present disclosure may be practiced in other ways than those described herein; it is to be understood that the embodiments disclosed in the specification are only a few embodiments of the present disclosure, and not all embodiments.
FIG. 1 is a schematic structural diagram of a detection system according to an embodiment of the present disclosure, FIG. 2 is a schematic structural diagram of a nucleic acid extraction device according to an embodiment of the present disclosure, and FIG. 3 is a rear view of the nucleic acid extraction device according to an embodiment of the present disclosure.
As shown in FIG. 1 to FIG. 3, the present disclosure provides a nucleic acid extraction apparatus, comprising a slide rail assembly 1 and a mechanical arm mechanism, wherein the mechanical arm mechanism is slidably connected to the slide rail assembly 1,
the mechanical arm mechanism comprises a base 2, a pipetting pump mechanical arm component 3 used for sucking the test solution and an extraction mechanical arm component used for extracting the nucleic acid, the base 2 is connected with the sliding rail component 1 in a sliding mode, and the pipetting pump mechanical arm component 3 and the extraction mechanical arm component are connected with the base 2 in a sliding mode along the sliding direction perpendicular to the base 2.
The nucleic acid extraction device provided by the embodiment of the disclosure comprises a mechanical arm mechanism and a sliding rail assembly 1 which are connected in a sliding manner, wherein the mechanical arm mechanism is connected with the sliding rail assembly 1 in a sliding manner through a base 2, a pipetting pump mechanical arm assembly 3 and an extraction mechanical arm assembly are arranged on the base 2, the pipetting pump mechanical arm assembly 3 and the extraction mechanical arm assembly are both connected with the base 2 in a sliding manner along the direction perpendicular to the base 2, namely the pipetting pump mechanical arm assembly 3 and the extraction mechanical arm assembly move along the direction perpendicular to the plane of the base 2. When nucleic acid detection is carried out, the pipetting pump mechanical arm assembly 3 moves along the direction vertical to the plane of the base 2 to finish the suction and adding actions of nucleic acid samples, reagents and sealing oil, then the base 2 slides relative to the sliding rail assembly 1 to adjust the relative positions of the extraction mechanical arm assembly and the nucleic acid samples, so that the extraction mechanical arm assembly and the samples are oppositely arranged, and then the extraction mechanical arm assembly is controlled to move along the direction vertical to the plane of the base 2 to realize the extraction of the nucleic acid. When the extracted nucleic acid needs to be transferred into an amplification instrument, after the extraction mechanical arm assembly finishes extracting the nucleic acid, the base 2 is controlled to move along the sliding rail assembly 1 so that the mechanical arm mechanism moves to the amplification instrument, then the extraction mechanical arm assembly moves towards the direction close to the base 2, the extracted nucleic acid is placed into the amplification instrument, and the amplification process of the nucleic acid is finished.
Through setting up slide rail set spare 1, base 2, setting up pipetting pump mechanical arm subassembly 3 on base 2 and drawing mechanical arm subassembly, realized the automation of drawing and the amplification process of nucleic acid, avoided nucleic acid to draw and amplify the manual intervention of in-process to avoid manual intervention to the influence of testing result, effectively improved application of sample stability, the uniformity of testing result and the accuracy of testing result.
In some embodiments, the extraction robot assembly comprises a magnetic rod robot assembly 4, the magnetic rod robot assembly 4 comprising a magnetic rod 41 for attracting magnetic beads and a first driving assembly 42 for driving the magnetic rod 41 to move in a sliding direction perpendicular to the base 2.
The nucleic acid to be extracted is attached to the surface of the magnetic bead, and the magnetic bead is taken out from the reagent by the magnetic rod 41, so that the extraction of the nucleic acid is realized. In the above embodiment, the first driving assembly 42 controls the magnetic rod 41 to move toward or away from the base 2, so as to control the magnetic rod 41 to extend into the test solution for attracting magnetic beads, and then move away from the test solution, so as to complete the extraction of nucleic acids.
When nucleic acid needs to be purified for multiple times, the first driving assembly 42 controls the magnetic rod 41 to reciprocate along the direction perpendicular to the base 2, and the magnetic beads are placed and taken out in a plurality of reagent kits by matching with the movement between the base 2 and the slide rail assembly 1.
Specifically, the magnetic rod robot arm assembly 4 further comprises a first connecting arm 43, one end of the first connecting arm 43 is connected with the first driving assembly 42, and the end of the other end is provided with the magnetic rod 41.
The first driving assembly 42 may include a motor and a screw assembly, the motor drives the screw to rotate, so that the screw nut moves along the screw, and the first connecting arm 43 is connected with the screw nut, so as to drive the magnetic bar 41 to perform a reciprocating motion.
In some embodiments, the bar magnet robot assembly 4 further comprises a first guiding assembly 44 for limiting the moving direction of the bar magnet 41, the first guiding assembly 44 is disposed on the base 2, and the first connecting arm 43 is connected to the first guiding assembly 44.
The first guide assembly 44 may be a slide rail and slide block assembly, connects the slide rail with the base 2, makes the slide rail perpendicular to the base 2, connects the first connecting arm 43 with the slide block, and realizes the limitation of the moving direction of the first connecting arm 43 through the cooperation of the slide block and the slide rail, thereby playing a role in guiding.
In some embodiments, the extraction robot assembly comprises a bar magnet sleeve robot assembly 5, the bar magnet sleeve robot assembly 5 comprises a bar magnet sleeve adapter sleeve 51 for sleeve-fitting with the bar magnet 41 and a second driving assembly 52 for driving the bar magnet sleeve adapter sleeve 51 to move along a sliding direction perpendicular to the base 2, and the bar magnet sleeve adapter sleeve 51 is sleeved outside the bar magnet 41.
The second driving assembly 52 drives the magnetic rod sleeve adapter sleeve 51 to move along the direction perpendicular to the sliding direction of the base 2, so that the magnetic rod sleeve adapter sleeve 51 picks up the magnetic rod 41 sleeve, and the matching between the magnetic rod 41 sleeve and the magnetic rod sleeve adapter sleeve 51 is completed. In addition, in the detection process, the second driving assembly 52 drives the magnetic rod 41 to move so as to uniformly mix the test solution, which is beneficial to the purification of nucleic acid.
After the transfer of nucleic acid is completed, when the magnetic rod 41 sleeve needs to be discarded, the first driving component controls the magnetic rod 41 to move towards the direction close to the base 2, or the second driving component controls the magnetic rod sleeve adapter sleeve 51 to move towards the direction far away from the base 2, so that the part of the magnetic rod 41 extending out of the magnetic rod sleeve adapter sleeve 51 is lengthened, the magnetic rod 41 sleeve is ejected, and the separation of the magnetic rod 41 sleeve and the magnetic rod sleeve adapter sleeve 51 is realized.
In some embodiments, the bar magnet sleeve mechanical arm assembly 5 further comprises a second connecting arm 53, one end of the second connecting arm 53 is connected to the second driving assembly 52, and the end of the other end is provided with the bar magnet sleeve adapter sleeve 51.
The second driving assembly 52 may include a motor and a screw assembly, the motor drives the screw to rotate, so as to enable the screw nut to move along the screw, and the second connecting arm 53 is connected with the screw nut, so as to drive the magnetic rod sleeve adapter sleeve 51 to move back and forth, so as to achieve the picking-up of the magnetic rod sleeve adapter sleeve 51 to the magnetic rod 41 sleeve.
Moreover, through the matching between the magnetic rod 41 and the magnetic rod sleeve adapter sleeve 51, the part of the free end of the magnetic rod 41 extending out of the magnetic rod sleeve adapter sleeve 51 can realize the picking function of the sealing cover.
Specifically, the magnetic rod 41 can pick up the sealing cover by matching with the sealing cover, such as clamping or inserting, and after the sealing cover is placed at a preset position, the second connecting arm 53 is moved towards the direction close to the sealing cover to press the sealing cover, so that the sealing cover forms a seal. The bar magnet 41 is then moved away from the seal cover to disengage the bar magnet 41 from the seal cover, which keeps the bar magnet cover robot assembly 5 in abutment with the seal cover to avoid seal failure as the seal cover moves with the bar magnet 41. Finally, the magnetic rod 41 is sleeved on the mechanical arm to move towards the direction far away from the sealing cover, so that the sealing function of the sealing cover is completed.
In some embodiments, the bar magnet robot assembly 4 further comprises a second guiding assembly 54 for limiting the moving direction of the bar magnet sleeve adapter sleeve 51, the second guiding assembly 54 is disposed on the base 2, and the second connecting arm 53 is connected to the second guiding assembly 54.
The second guide assembly 54 may be a sliding rail and sliding block assembly, connects the sliding rail with the base 2, makes the sliding rail perpendicular to the base 2, connects the second connecting arm 53 with the sliding block, and realizes the limitation of the moving direction of the second connecting arm 53 by the cooperation of the sliding block and the sliding rail, thereby playing a role in guiding.
In some embodiments, the side of the second connecting arm 53 facing the susceptor 2 is provided with a reaction cup thermal cover 55 and a heating assembly.
The reaction kit is pressed and fixed by the reaction cup hot cover 55 in the reaction process, and the temperature of the reaction cup hot cover 55 is controlled to be 105 ℃ by the heating component matched with the reaction cup hot cover 55 in the reaction process, so that the reaction liquid is prevented from volatilizing upwards in the high-temperature reaction of nucleic acid detection.
In some embodiments, the pipetting pump robot arm assembly 3 comprises a pipetting pump assembly 31 for aspirating the test solution and a third drive assembly 32 for driving the pipetting pump assembly 31 to move in a sliding direction perpendicular to the base 2.
Specifically, the pipetting pump mechanical arm assembly 3 further comprises a third connecting arm 33, one end of the third connecting arm 33 is connected with the third driving assembly 32, and the end of the other end is provided with the pipetting pump assembly 31.
Above-mentioned third drive assembly 32 can include motor and lead screw assembly, and the motor drive lead screw rotates to make screw nut along the lead screw motion, third linking arm 33 is connected with screw nut, thereby drives and move liquid pump assembly 31 and realize reciprocating motion, with the absorption and the interpolation action of realization liquid pump assembly 31 to nucleic acid sample, reagent and sealing oil.
Specifically, the pipette assembly 31 includes an adapter 311 for picking up a pipette tip, and the pipette tip is picked up by the adapter 311.
In some embodiments, the pipetting pump mechanical arm assembly 3 further comprises a third guide assembly 34 for limiting the movement direction of the pipetting pump assembly 31, the third guide assembly 34 is disposed on the base 2, and the third connecting arm 33 is connected with the third guide assembly 34.
Above-mentioned third direction subassembly can be for slide rail sliding block set spare, is connected slide rail and base 2 to make the slide rail perpendicular with base 2, be connected third linking arm 33 and slider, through the cooperation of slider with the slide rail, realize the restriction to third linking arm 33 direction of motion, thereby play the guide effect.
In some embodiments, the slide assembly 1 includes a driving member for driving the slide along the slide base, and the base 2 is connected to the slide.
In order to guarantee the stability of 2 movements of base, be provided with two at least parallel slide rails on the slide rail base, every slide rail all with 2 sliding connection of base.
The embodiment of the disclosure also provides a detection system, which comprises the nucleic acid extraction device.
Through setting up nucleic acid extraction element, realized detecting system's automation, avoided the artifical intervention of nucleic acid extraction with the amplification in-process to avoid artifical the influence to the testing result, effectively improved application of sample stability, the uniformity of testing result and the accuracy of testing result.
Specifically, the device further comprises an electronic control system 6 and a fluorescence testing component, wherein the electronic control system 6 is electrically connected with the nucleic acid extraction device.
Specifically, the electronic control system 6 is electrically connected to the first driving assembly 42, the second driving assembly 52, the third driving assembly 32 and the pipetting pump assembly 31 to control the above-mentioned assemblies.
In some embodiments, a kit component 7 and a thermal cycling component 8 are also included, the thermal cycling component 8 being used to effect amplification reactions and detection of nucleic acids.
Specifically, the fluorescence test component is connected with the thermal cycle component 8 through an optical fiber, so as to realize the excitation of fluorescence and the acquisition of a fluorescence signal.
It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The foregoing are merely exemplary embodiments of the present disclosure, which enable those skilled in the art to understand or practice the present disclosure. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the disclosure. Thus, the present disclosure is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A nucleic acid extraction device is characterized by comprising a slide rail component (1) and a mechanical arm mechanism, wherein the mechanical arm mechanism is connected with the slide rail component (1) in a sliding way,
the mechanical arm mechanism comprises a base (2), a pipetting pump mechanical arm assembly (3) used for sucking a test solution and an extraction mechanical arm assembly used for extracting nucleic acid, wherein the base (2) is connected with the sliding rail assembly (1) in a sliding manner, and the pipetting pump mechanical arm assembly (3) and the extraction mechanical arm assembly are connected with the base (2) in a sliding manner along a direction perpendicular to the base (2).
2. The nucleic acid extraction apparatus according to claim 1, wherein the extraction robot assembly comprises a magnetic rod robot assembly (4), the magnetic rod robot assembly (4) comprising a magnetic rod (41) for aspirating magnetic beads and a first driving assembly (42) for driving the magnetic rod (41) to move in a sliding direction perpendicular to the base (2);
the magnetic bar mechanical arm assembly (4) further comprises a first connecting arm (43), one end of the first connecting arm (43) is connected with the first driving assembly (42), and the end part of the other end is provided with the magnetic bar (41).
3. The nucleic acid extraction apparatus according to claim 2, wherein the magnetic rod robot assembly (4) further comprises a first guide assembly (44) for limiting the movement direction of the magnetic rod (41), the first guide assembly (44) is provided to the base (2), and the first connecting arm (43) is connected to the first guide assembly (44).
4. The nucleic acid extraction device according to claim 2, wherein the extraction mechanical arm assembly comprises a magnetic rod sleeve mechanical arm assembly (5), the magnetic rod sleeve mechanical arm assembly (5) comprises a magnetic rod sleeve adapter sleeve (51) used for being matched with a magnetic rod sleeve and a second driving assembly (52) used for driving the magnetic rod sleeve adapter sleeve (51) to move along a sliding direction perpendicular to the base (2), and the magnetic rod sleeve adapter sleeve (51) is sleeved on the outer side of the magnetic rod (41).
5. The nucleic acid extraction apparatus according to claim 4, wherein the magnetic rod sleeve mechanical arm assembly (5) further comprises a second connecting arm (53), one end of the second connecting arm (53) is connected to the second driving assembly (52), and the end of the other end is provided with the magnetic rod sleeve adapter sleeve (51).
6. The nucleic acid extraction apparatus according to claim 5, wherein the magnetic rod mechanical arm assembly (4) further comprises a second guide assembly (54) for limiting the movement direction of the magnetic rod sleeve adapter sleeve (51), the second guide assembly (54) is disposed on the base (2), and the second connecting arm (53) is connected to the second guide assembly (54).
7. The nucleic acid extraction apparatus according to claim 5, wherein a reaction cup heat cover (55) is provided on a side of the second connection arm (53) facing the base (2).
8. The nucleic acid extraction apparatus according to claim 1, wherein the pipette pump robot arm assembly (3) comprises a pipette pump assembly (31) for aspirating a test solution and a third drive assembly (32) for driving the pipette pump assembly (31) to move in a sliding direction perpendicular to the base (2);
the pipetting pump assembly (31) comprises an adapter (311) for picking up pipette tips.
9. A detection system comprising the nucleic acid isolation apparatus according to any one of claims 1 to 8.
10. The detection system according to claim 9, further comprising an electronic control system (6), a reagent kit component (7) and a thermal cycling component (8), wherein the electronic control system (6) is electrically connected with the nucleic acid extraction device, and the thermal cycling component (8) is used for realizing amplification reaction and detection of nucleic acid.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410429A (en) * | 2022-02-08 | 2022-04-29 | 深圳潘道医学检验实验室 | High-purification-degree automatic nucleic acid extraction instrument |
| CN114480117A (en) * | 2021-12-27 | 2022-05-13 | 宁波爱基因科技有限公司 | Nucleic acid automatic extraction instrument and nucleic acid extraction method |
| CN114736766A (en) * | 2022-04-08 | 2022-07-12 | 吉林大学 | Nucleic acid extraction system |
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