CN113475505B - Composition for regulating growth of turfgrass and improving stress resistance and preparation method and application thereof - Google Patents

Abstract

The invention relates to a composition for regulating lawn grass growth and improving stress resistance, and a preparation method and application thereof. The weight ratio of coronatine to prohexadione calcium is 0.0001-0.01: 1 to 20. The field test proves that the composition provided by the invention has good vigorous-growth-controlling and stress-resisting effects on turfgrass. The composition can adjust the type of the lawn grass, reduce the times of manual trimming, improve the low temperature resistance and drought resistance of the lawn grass, effectively reduce the labor input, and simultaneously avoid the damage to the lawn grass caused by excessive trimming. Compounding coronatine and prohexadione calcium according to a certain proportion finds that the activity of a mixed preparation is greatly improved compared with that of a single preparation, and the mixed preparation has great development value compared with a single preparation.

Description

A composition for regulating turfgrass growth and improving stress resistance and its preparation method and application

technical field

The invention belongs to the technical field of plant growth regulators, in particular to a regulator composition for regulating the growth of turfgrass and improving stress resistance, and a preparation method and application thereof.

Background technique

In recent years, with the improvement of material, culture and living standards, people's requirements for environmental beautification are also getting higher and higher An important part of the construction of ecological civilization, it plays a very important role in the greening of the urban environment. In the process of lawn maintenance, maintenance and management fees mainly come from pruning, irrigation, fertilization, weeding and pest control, among which pruning and irrigation account for the largest proportion. Pruning is the premise to ensure the good quality of turfgrass. However, frequent pruning will cause a lot of grass clippings, but also a lot of nutrients will be lost, and more importantly, it will consume a lot of manpower and material resources.

Foreign scholars have done a lot of research on reducing lawn mowing times, reducing management costs, and improving lawn quality. Among them, the use of growth retarders to control lawn growth has made great progress. In China, artificial pruning is mainly used, and the research on the application of plant growth regulators on lawns is relatively weak, mainly focusing on chlorophyll, ethephon, and triazole compounds. However, these hormones not only have different concentrations in different plants and different environments, but also it is difficult to grasp the appropriate concentration when using them, which often causes damage or destruction of turfgrass. Therefore, until now, these plant growth regulators have rarely been widely used in lawn maintenance.

Procyclonic acid calcium is a plant growth retarder, which has the functions of inhibiting plant growth, promoting root growth, shortening internodes, enhancing lodging resistance, increasing chlorophyll content, and improving photosynthetic capacity. It has a significant effect on the control of turfgrass plant type, and the inhibition rate of tall fescue can reach 95.2% under the dosage of 0.36kg/hm ² .

Coronatine (Coronatine, hereinafter referred to as COR) is a metabolite produced by the phytopathogenic strain of Pseudomonas syringae, which was discovered in the 20th century. Coronasine has a variety of functions to regulate plant growth, such as shortening internodes, promoting the formation and expansion of tubers, inhibiting seed germination and root growth, and promoting fruit shedding.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a kind of composition that regulates the growth of turfgrass to improve stress resistance and its preparation method and the method of application, and the active ingredients are coronatine and prohexadione calcium, the complex of coronatin and prohexadione calcium. The compound composition has the functions of regulating the growth of turfgrass and improving the cold resistance and drought resistance of the turfgrass.

The composition provided by the present invention, its active ingredients include coronatine and prohexadione calcium;

The weight ratio of coronatine and prohexadione calcium is 0.0001-0.01:1-20.

In the above composition, the weight ratio of coronatine and prohexadione calcium is 0.0001～0.01:1, 0.0001～0.01:20, 1:10000～1:200000, 1:10000～1:100000, 1:10000 ~1:50000, 1:100-5000, 1:100-1000, 1:100-500, 1:100-200;

Specifically, 0.006%: 20%, 0.006%: 5%, 0.012%: 10%, 0.012%: 5%, 0.001%: 20%, 0.001%-0.006%: 5-20%, 0.001%-0.006%: 20 %, 0.1%: 5-8%, 0.006%: 8-10%, 0.05%: 5%, 0.012%: 10% or 0.001%-0.006%: 5%.

In addition, plant growth regulating preparations comprising the above compositions also belong to the protection scope of the present invention.

Specifically, the dosage form of the plant growth regulating preparation can be any dosage form allowed in agriculture, and can be selected from at least one of a solution, a water dispersible granule, a wettable powder, a suspension and an effervescent tablet. .

The plant growth regulating preparation also contains adjuvant;

The auxiliary agents are all conventional auxiliary agents, and are specifically selected from solvents, emulsifiers, antifreeze agents, preservatives, defoaming agents, dispersants, wetting agents, disintegrating agents, binders, carriers, thickeners, water blocking agents. At least one of an agent, an acidic effervescent agent, an alkaline effervescent agent and a lubricant.

The solvent is selected from ethanol, propanol, isopropanol, propylene glycol, glycerol, n-butanol, isobutanol, tert-butanol, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol dimethyl ether, At least one of ethyl acetate, mineral oil, aromatic hydrocarbon solvent oil and methyl oleate;

The emulsifier is various conventional surfactants, and can be selected from at least one of cationic surfactants, anionic surfactants, nonionic surfactants and zwitterionic surfactants;

The antifreeze is selected from at least one of ethylene glycol, glycerol, 1,2-propylene glycol, polyethylene glycol, urea, sorbitol and sodium chloride;

The preservative is selected from at least one of sodium sorbate, benzoic acid, WINN 0044, sorbic acid, sodium benzoate, benzaldehyde, p-hydroxybenzaldehyde, sodium salicylate and 2-hydroxybiphenyl;

The defoamer is selected from at least one of organosilicon defoamer, polyether defoamer or polyether modified polysiloxane defoamer;

The dispersant is selected from Tersperse 2425, Tersperse 2500, polycarboxylate, naphthalene sulfonate, lignosulfonate, fatty alcohol polyoxyethylene ether phosphate, alkyl naphthalene sulfonate, alkylphenol polyoxyethylene Ether, alkylphenol polyoxyethylene ether sulfonate, NNO, sodium dodecylbenzene sulfonate, alkyl polyoxyethylene ether sulfonate and alkylphenol polyoxyethylene ether formaldehyde condensate and SP-SC3 at least one;

The wetting agent is selected from naphthalene sulfonate, fatty alcohol sulfate, dodecylbenzene sulfonate, sodium dodecyl sulfate, fatty alcohol polyoxyethylene ether, alkyl sulfonate, secondary alkyl sulfate Sodium, fatty alcohol polyoxyethylene ether, nonylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, Tersperse 2500, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene At least one of base ether phosphate, alkyl sulfate, naphthalene sulfonate, fatty alcohol sulfate, water-soluble chitosan, calcium stearate, magnesium stearate and talc;

The disintegrant is selected from at least one of ammonium sulfate, urea, corn starch and derivatives thereof, low-substituted hydroxypropyl cellulose, polyvinylpyrrolidone and sodium alginate;

The binder is selected from at least one of hydroxyethyl cellulose, soluble starch, polyethylene glycol, methyl cellulose, dextrin, fructose and polyvinyl alcohol;

The carrier is selected from at least one of silica, kaolin, bentonite, diatomite, light calcium carbonate, gypsum, attapulgite, talc, montmorillonite, urea and sucrose;

The thickening agent is selected from at least one of xanthan gum, polyvinyl alcohol sodium alginate, carboxymethyl cellulose, guar gum, sodium polyacrylate, organic bentonite and magnesium aluminum silicate;

The antifreeze is selected from at least one of ethylene glycol, glycerol, 1,2-propylene glycol, polyethylene glycol, urea, sorbitol and sodium chloride;

The preservative is selected from at least one of sodium sorbate, benzoic acid, WINN 0044, sorbic acid, sodium benzoate, benzaldehyde, p-hydroxybenzaldehyde, sodium salicylate and 2-hydroxybiphenyl;

The defoamer is selected from at least one of organosilicon defoamer, polyether defoamer and polyether modified polysiloxane defoamer;

The water blocking agent is selected from at least one of calcium hydrogen phosphate, white carbon black, calcium carbonate, bentonite, diatomite and aluminum oxide;

The acidic effervescent agent is selected from at least one of citric acid, tartaric acid, oxalic acid, maleic acid, fumaric acid, malic acid, succinic acid and adipic acid;

The alkaline effervescent agent is selected from at least one of sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, calcium carbonate and ammonium bicarbonate;

The salts are sodium or calcium salts such as polycarboxylates, lignosulfonates and the like.

The sum of the mass of coronatine and procyclonic calcium accounts for 1.0001%-20.01% of the total mass of the plant growth regulating preparation; specifically, it can be 10.006%, 5.006%, 5.012%, 5.05%, 5.1%, 8.006% , 8.1%, 10.006%, 10.012% or 20.001%.

In addition, the application of the composition provided by the present invention or the plant growth regulating preparation in the following at least one also belongs to the protection scope of the present invention:

a. Adjust the plant type;

b. Improve plant abiotic stress resistance;

c. Improve plant yield;

d. Promote plant growth.

Specifically, the plant is cotton;

The abiotic stress resistance is drought resistance and/or cold resistance;

The regulating plant type is inhibiting plant leggy.

The application method of the composition or the plant growth regulating preparation is a spray method, and other application techniques applied in agriculture can also be adopted as required.

Field tests have proved that the composition provided by the invention has a good effect of controlling the growth of turfgrass and resisting stress. The composition can adjust the plant type of turfgrass, reduce the frequency of manual trimming, improve the low temperature and drought resistance activity of the turfgrass, effectively reduce the input of labor, and at the same time can avoid the damage to the turfgrass caused by excessive pruning. Combining coronatine and prohexadione calcium in a certain proportion, it is found that the activity of the mixture is greatly improved than that of the single agent, and there is a huge development value compared with the single dosage form.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments. The methods are conventional methods unless otherwise specified. The raw materials can be obtained from open commercial sources unless otherwise specified. The percentages in the following examples are by weight.

Example 1

Preparation of 10.006% coronatine·procyclonic calcium water dispersible granules

Coronasine TC (100%) 0.006%, Procyclonic Calcium TC (100%) 20%, Terspers2425 (produced by Huntsman, USA, alkylnaphthalene sulfonate) 5%, Tersperse2500 (Huntsman, USA) Mai company produced, alkyl naphthalene sulfonates) 2% urea 5%, soluble starch 5%, xanthan gum 1%, ammonium sulfate 15%, kaolin to make up to 100%; add the above materials by weight percentage into the mixer Mixed evenly, pulverized by a jet mill, kneaded by adding an appropriate amount of water, the kneaded material was granulated by an extrusion granulator, dried and sieved to obtain 10.006% coronatine·procyclonic calcium water dispersible granules agent.

Example 2

Preparation of 5.006% coronatine·prohexadione calcium wettable powder

Coronasine original drug (100%) 0.006%, prohexadione calcium original drug (100%) 5%, sodium dodecylbenzene sulfonate 5%, NNO3%, light calcium carbonate to make up to 100%; The materials are added to the mixer according to the weight percentage and mixed evenly, then pulverized by the jet mill, and then fully mixed in the mixing kettle to prepare 5.006% coronatine and procyclocylic acid calcium wettable powder. Suspensions of any desired concentration can be diluted with water.

Example 3

Preparation of 10.012% coronatine·prohexadione calcium suspending agent

Coronasin original drug (folded) 0.012%, prohexadione calcium original medicine (folded) 10%, SP-SC3 5%, sodium lignosulfonate 3%, xanthan gum 0.3%, ethylene glycol 5% , 0.2% silicone defoamer, 0.5% sodium benzoate, and water to 100%; add the above materials by weight percentage into the mixer and mix evenly, fully grind in a sand mill, and then make 10.012% coronatine · Prohexadione calcium suspending agent. Suspensions of any desired concentration can be diluted with water.

Example 4

Preparation of 5.012% coronatine·procyclonic calcium effervescent tablet

Coronasin TC (100%) 0.012%, Mepiperinium TC (100%) 5%, Sodium Lignin Sulfonate 5%, Sodium Lauryl Sulfate 3%, Urea 5%, Hydroxyethyl Cellulose 3%, white carbon black is supplemented to 100%; the above materials are added to the mixer according to the weight percentage and mixed evenly, pulverized by a jet mill, then fully mixed in a mixing kettle, and pressed by a tablet machine to obtain 5.012% crown. Bacteriocin · prohexadione calcium tablets.

Example 5

Preparation of 20.001% coronatine·prohexadione calcium effervescent tablet

Coronasin TC (100%) 0.001%, Mepiperinium TC (100%) 20%, Calcium Hydrogen Phosphate 10%, Citric Acid 5%, Potassium Carbonate 5%, Soluble Starch 10%, Polycarboxylate 5 %, 5% of talc, and 100% of bentonite; add the above materials by weight percentage into a mixer and mix evenly, pulverize by a jet mill, then fully mix in a mixing kettle, and press into a tablet machine to obtain 20.001 % coronatine · prohexadione calcium effervescent tablet.

Experiment on Inhibition of Growth of Indoor Potted Turfgrass

According to the steps of Example 3, only replace the ratio of the former drug of coronatine and the former drug of prohexadione calcium, obtain the composition of different contents and single-agent treatment crops, correspondingly handle 1-7, by comparing the plant height inhibition rate and drought The rationality of mixing is measured by the rehydration survival rate after stress. The formula for calculating the plant height inhibition rate is:

Example 3 and other treatments were prepared according to the same method of use as above, turfgrass was trimmed to a uniform height at the seedling stage, and the preparation was diluted 1500 times for foliar spraying, with an interval of 15 days, and the application was 2 times; the blank group was treated with water ; 15 days after the second application, the plant height was measured, and the test results were shown in Table 1.

Table 1. Effects of coronatine and prohexadione calcium on the plant height of green grass

It can be seen from Table 1 that treatment 3 (ie 0.012% COR·10% prohexadione calcium) has the highest plant height inhibition rate, up to 95.6%, followed by treatment 2 (ie 0.1% COR · 8% prohexadione) calcium), was 90.1%.

Drought-resistant growth test of indoor potted turfgrass

According to the step of embodiment 3, only replace the ratio of the former medicine of coronatine and the former medicine of prohexadione calcium, obtain the compositions of different contents and single-agent treatment crops, correspondingly handle 1-7 (the former medicine of coronatine in each treatment) The ratio of the original drug of prohexadione calcium is the same as that in Table 1), turfgrass was trimmed to a uniform height at the seedling stage, and the preparation was diluted 1500 times for foliar spraying, with an interval of 7 days, and the application was twice; the blank group was treated with water ; Begin to carry out drought stress 7 days after spraying, and count the survival rate of turfgrass when drought stress is 7 days. The test results are shown in Table 2.

Table 2 Effects of coronatine and prohexadione calcium compound preparation on drought resistance of green grass seedlings

deal with Survival rate (%) CK 5.3 0.01%COR 10.3 1# 73.7 2# 88.1 3# 93.6 4# 69.3 5# 73.3 6# 76.8 7# 75.9

It can be seen from Table 2 that treatment 3 (0.012% COR·10% prohexadione calcium) has the highest plant height inhibition rate, up to 93.6%, followed by treatment 2 (that is, 0.1% COR·8% prohexadione). calcium), was 88.1%.

The above-mentioned field test proves that the composition provided by the present invention has a good effect of controlling the growth of turfgrass and resisting stress. The composition can adjust the plant type of turfgrass, reduce the frequency of manual trimming, improve the low temperature and drought resistance activity of the turfgrass, effectively reduce the input of labor, and at the same time can avoid the damage to the turfgrass caused by excessive pruning. Combining coronatine and prohexadione calcium in a certain proportion, it is found that the activity of the mixture is greatly improved than that of the single agent, and there is a huge development value compared with the single dosage form.

Claims (6)

1. a plant growth regulating preparation, its active ingredients are coronatine and prohexadione calcium; In the plant growth regulating preparation, the content of the coronatine and prohexadione calcium is any of the following: 1) The coronatine accounts for 0.1% of the total mass of the plant growth regulating preparation, and the prohexadione calcium accounts for 5% of the total mass of the plant growth regulating preparation; 2) The coronatine accounts for 0.1% of the total mass of the plant growth regulating preparation, and the prohexadione calcium accounts for 8% of the total mass of the plant growth regulating preparation; 3) The coronatine accounts for 0.012% of the total mass of the plant growth regulating preparation, and the prohexadione calcium accounts for 10% of the total mass of the plant growth regulating preparation. 2. The plant growth regulating preparation according to claim 1, characterized in that: the dosage form of the plant growth regulating preparation is selected from the group consisting of at least a soluble agent, a water dispersible granule, a wettable powder, a suspending agent and an effervescent tablet. A sort of. 3. The plant growth regulating preparation according to claim 2, characterized in that: the plant growth regulating preparation also contains an adjuvant; The auxiliary agent is selected from the group consisting of solvent, emulsifier, antifreeze agent, preservative, defoamer, dispersant, wetting agent, disintegrant, binder, carrier, thickener, water blocking agent, acidic effervescent agent, At least one of an alkaline effervescent agent and a lubricant. 4. The application of the plant growth regulating preparation of any one of claims 1-3 in inhibiting plant growth. 5. The application according to claim 4, wherein the plant is turfgrass. 6. The application according to claim 5, wherein the turfgrass is selected from at least one of warm-season and cool-season turfgrass.