CN113403199A - Online real-time detection system and method for biological sample - Google Patents

Abstract

The present application provides an online real-time detection system and detection method for biological samples. The online real-time detection system for biological samples provided by the present application includes: a culture container; a sampling pipe fitting, which is used for sampling a sample to be detected from the culture container to a quantitative component; the quantitative component includes a quantitative structure, and the quantitative structure is used to determine the sampling from the culture container. The amount of the sample to be detected to the quantitative component; the sampling host, including the main pump, the main pump is used to drive the sample to be detected from the culture vessel to the quantitative component and to drive the sample to be detected determined by the quantitative structure to the analyzer; analysis; The instrument is used to receive the sample to be tested and analyze the sample to obtain the analysis result; the feeding component is connected to the feeding port of the culture vessel through the pipeline; the control part, the main pump, the analyzer and the feeding component are all connected with the control The part of the signal is connected, and the control part is used to obtain the analysis result and control the feeding component to feed the culture vessel according to the analysis result.

Description

A system and method for online real-time detection of biological samples

technical field

The present application belongs to the technical field of biological sample detection, and more particularly, relates to an online real-time detection system and method for biological samples.

Background technique

In the prior art, in industries such as microbial culture and cell culture, when the culture medium is tested, the sample needs to be manually taken out from the culture container first, and then the sample is put into a centrifuge for centrifugation, and then the supernatant is taken out. Manually transfer the supernatant to an analytical device for detection. After the detection result is obtained through detection, it is then judged whether to feed the culture medium in the container. If feeding is required, the feeding equipment is manually controlled to feed.

Specifically, microbial culture, cell culture and other industries currently use culture tanks made of glass or stainless steel, and the size of the tank varies from a few milliliters to several hundred liters. Manually take out the medium sample from the culture tank. Before sampling, it is necessary to inject high-pressure steam into the sampling port for 30 minutes of sterilization, and then open the sampling port to release the samples in the front section, and take the samples in the middle and rear sections for rapid packaging. After the sampling is completed, it is necessary to inject high-pressure steam into the sampling port for sterilization for 30 minutes. The sample taken out is moved to a centrifuge for centrifugation, and the supernatant is taken to an analysis device for detection and analysis. Generally, centrifuge equipment and analytical equipment are located in other laboratories, and there are unavoidable gaps in space and time. After the operator obtains the test results of the sample, he or she determines whether to feed the material according to the calculation.

The above-mentioned prior art has the following technical defects:

First, the sterilization operation before and after sampling is cumbersome and time-consuming, which cannot meet the requirement of high sampling frequency;

Second, the entire detection process includes manual sampling, centrifugation, detection, and feeding, which takes a long time, and the component content of the tested item in the culture tank is constantly changing. The content of the test items may have changed, thus causing the feeding to fail to achieve the expected effect;

Third, the amount of manual sampling cannot be precisely controlled, and multiple sampling and monitoring cannot be achieved for the cell culture industry with less culture medium;

Fourth, the entire existing detection process requires manual participation in the operation, which requires a lot of labor costs.

SUMMARY OF THE INVENTION

The purpose of the embodiments of the present application is to provide an online real-time detection system and detection method for biological samples, and the detection system and detection method have the advantages of accurate sampling, simplified procedures, and integrated control.

In order to achieve the above-mentioned purpose, the technical scheme adopted in this application is to provide a biological sample online real-time detection system, which includes:

A culture container for containing the sample to be tested;

A sampling pipe, the first end of the sampling pipe is used to extend below the liquid level of the sample to be detected, the second end of the sampling pipe is connected to the quantitative component, and the sampling pipe is used to make the sample to be detected sampling the culture vessel to the quantitative component;

The quantitative component includes a quantitative structure, the first end of the quantitative structure is connected to the second end of the sampling pipe, the second end of the quantitative structure is connected to the sampling host, and the quantitative structure is used to determine the the amount of the sample to be detected sampled from the culture vessel to the quantitative component;

The sampling host includes a main pump, the main pump is connected between the quantitative component and the analyzer through a pipeline, and the main pump is used to drive the sample to be detected from the culture vessel to the quantitative component and use it. in driving the sample to be detected determined by the quantitative structure to the analyzer;

the analyzer, configured to receive the sample to be detected and analyze the sample to be detected to obtain an analysis result;

a feeding component, connected to the feeding port of the culture vessel through a pipeline;

A control part, wherein the main pump, the analyzer and the feeding component are all signal-connected to the control part, and the control part is used for acquiring the analysis result and controlling the feeding component according to the analysis result The culture vessel is fed.

In one embodiment, the biological sample online real-time detection system further includes a sample receiving component;

The sample receiving assembly includes a receiving tank and a sample transfer needle connected to the receiving tank, the receiving tank is connected to the main pump, and the sample transfer needle is connected to the analyzer.

In one embodiment, the quantitative component further includes a first three-way solenoid valve, a second three-way solenoid valve, a sterilizing container, and an air filter; wherein,

The normally closed end of the first three-way solenoid valve is connected to the second end of the sampling pipe, the normally open end of the first three-way solenoid valve is connected to the common end of the second three-way solenoid valve through a pipeline, and the The common end of the first three-way solenoid valve is connected to the first end of the quantitative structure;

The normally closed end of the second three-way solenoid valve is connected to the disinfection container through a pipeline, and the disinfection container is used to accommodate the disinfectant, and the normally open end of the second three-way solenoid valve is connected to the air filter through a pipeline. device;

Both the first three-way solenoid valve and the second three-way solenoid valve are signal-connected to the control part.

In one embodiment, the quantitative component further includes a first liquid detector and a second liquid detector; wherein,

The normally closed end of the second three-way solenoid valve is connected to the first port of the first liquid detector through a pipeline, and the disinfection container is connected to the second port of the first liquid detector through a pipeline;

The second end of the quantitative structure is connected to the first port of the second liquid detector, and the main pump is connected to the second port of the second liquid detector through a pipeline;

Both the first liquid detector and the second liquid detector are signal-connected to the control part.

In one embodiment, the sampling host further includes a solenoid valve, the second end of the quantitative structure is connected to the normally closed end of the solenoid valve through a pipeline, and the main pump is connected to the normally closed end of the solenoid valve through a pipeline At the end, the solenoid valve is signally connected to the control part.

In one embodiment, the sampling host includes at least two solenoid valves, and at least two normally closed ends of the at least two solenoid valves are connected to the main pump through pipes;

The biological sample online real-time detection system includes at least two of the sampling tubes, at least two quantitative components and at least two feeding components; at least two of the sampling tubes, at least two quantitative components and at least two feeding components Connect in one-to-one correspondence; at least two second ends of at least two of the quantitative structures in at least two of the quantitative assemblies and at least two normally closed ends of at least two of the solenoid valves are connected in a one-to-one correspondence.

In one embodiment, the sample receiving component further includes at least one of a drainage component, a water inlet component, and an overflow component; wherein,

The drainage assembly includes a drainage pipe, a first driving pump and a first collection container, the first end of the drainage pipe is connected to the receiving tank, the second end of the drainage pipe is connected to the first collection container, the first driving pump is arranged on the drainage pipe;

The overflow assembly includes an overflow pipe, a second driving pump and a second collection container, the first end of the overflow pipe is connected to the receiving tank, and the second end of the overflow pipe is connected to the first Two collection containers, the second driving pump is arranged on the overflow pipe;

The water inlet assembly includes a water inlet pipe, a third driving pump and a pure water container, the pure water container is used to accommodate pure water, the first end of the water inlet pipe is connected to the receiving tank, the water inlet The second end of the pipeline is connected to the pure water container, and the third driving pump is arranged on the water inlet pipeline;

The first drive pump, the second drive pump, and the third drive pump are all signal-connected to the control portion.

In one embodiment, the sample receiving assembly further includes a third liquid detector, the main pump is connected to the first port of the third liquid detector through a pipeline, and the sample receiving tank is connected to the first port through a pipeline. The second port of the three liquid detectors, the third liquid detector is signally connected to the control part.

In one embodiment, the sampling tube includes a probe tube, a membrane tube and a rotary valve; wherein,

The first end of the probe tube extends into the interior of the culture container, and the second end of the probe tube is connected to the quantitative component through a hose; the rotary valve is arranged on the hose close to the probe tube The membrane tube is connected to the first end of the probe tube, and the membrane tube is used to extend below the liquid level of the sample to be detected.

In one embodiment, the feeding component includes a feeding pipeline, a feeding pump and a feeding container, and the feeding container is used for containing the feeding liquid; wherein,

The first end of the feeding pipeline is connected to the feeding port of the culture vessel, and the second end of the feeding pipeline is used to extend below the liquid level of the feeding liquid; the feeding pump is arranged at On the feeding pipeline, the feeding pump is signally connected to the control part.

Compared with the prior art, the online real-time detection system for biological samples provided by the present application, through the combination of the control part and the quantitative component, determines the amount of the sample to be detected sampled from the culture vessel to the quantitative component in real time through the quantitative structure, and uses the main pump to determine the amount of the sample to be detected. The sample to be detected is driven from the culture container to the quantitative component in real time, and the sample to be detected determined by the quantitative structure is driven to the analyzer, and the sample to be detected is analyzed in real time by the analyzer to obtain the analysis result, and the real-time control of the control system is carried out. , the feeding component can be controlled in real time to feed the culture vessel according to the analysis results. which has the following

Therefore, the beneficial effects of the biological sample online real-time detection system provided by this application are:

First, the sampling procedure is simplified, the time from sampling to feeding is shortened, and the problem that the prior art cannot satisfy high-frequency and high-efficiency sampling because the sampling procedure is cumbersome and time-consuming;

Second, the single sampling amount can be precisely controlled, which solves the problem that the sampling amount cannot be accurate due to manual sampling in the prior art;

Fourth, the whole process is automated and integrated, which solves the problem of high labor costs caused by the need for a large number of manual participation in the entire monitoring process in the prior art.

Another object of the present application is to provide a detection method corresponding to the above-mentioned biological sample online real-time detection system, comprising:

Start and run the main pump through the control part, so that the main pump drives the sample to be detected in the culture vessel to the quantitative structure of the quantitative component through the sampling tube for quantitative determination;

The main pump drives the sample to be detected quantified by the quantitative structure to the analyzer from the quantitative component;

The analyzer receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result;

The control part obtains the analysis result from the analyzer and controls the feeding component to feed the culture vessel according to the analysis result.

In an embodiment, before the main pump drives the sample to be detected in the culture vessel to the quantitative structure of the quantitative component through the sampling tube for quantitative measurement, the method further includes:

the control part controls the solenoid valve to open the normally closed end of the solenoid valve and close the normally open end of the solenoid valve;

the control part controls the second three-way solenoid valve to open the normally closed end of the second three-way solenoid valve and close the normally open end of the second three-way solenoid valve;

The main pump drives the disinfectant in the disinfection container to the quantitative structure through the second three-way solenoid valve and the first three-way solenoid valve for quantitative measurement;

The quantitative structure completes the quantification of the disinfectant, the first liquid detector obtains a quantitative completion signal and sends it to the control part, and the control part controls the second three-way solenoid valve to close according to the quantitative completion signal. The normally closed end of the second three-way solenoid valve and the normally open end of the second three-way solenoid valve are opened;

The main pump drives the sterilizing solution quantified by the quantitative structure to the sample receiving assembly from the quantitative structure.

In one embodiment, after the main pump drives the sterilizing solution determined by the quantitative structure to the sample receiving assembly from the quantitative structure, the main pump drives the to-be-detected liquid in the culture container Before the sample is driven into the quantitative structure of the quantitative component by the sampling tube for quantitative measurement, the sample also includes:

The receiving tank of the sample receiving component receives the disinfectant and overflows, and the third liquid detector obtains the overflow signal and sends it to the control part, and the control part controls the second drive pump to send the overflow signal according to the overflow signal. The disinfectant in the overflow part is driven to the second collection container, and the control part controls the solenoid valve according to the overflow signal to close the normally closed end of the solenoid valve and open the normally open end of the solenoid valve;

The control part controls the first drive pump to drive the disinfectant in the receiving tank to the first collection container;

The control part controls the third driving pump to drive the pure water in the pure water container to the receiving tank, and the control part controls the second driving pump to drive the pure water in the receiving tank to the second collecting tank in the container.

In one embodiment, the control part controls the third driving pump to drive the pure water in the pure water container into the receiving tank, and the control part controls the second driving pump to drive the receiving tank After the pure water in the pool is driven into the second collection container, the main pump drives the sample to be detected in the culture container to the quantitative structure of the quantitative component through the sampling pipe Before quantification, also include:

The control part controls the first three-way solenoid valve to open the normally closed end of the second three-way solenoid valve and close the normally open end of the second three-way solenoid valve.

In one embodiment, after the main pump drives the sample to be detected in the culture container to the quantitative structure of the quantitative component through the sampling pipe for quantitative measurement, the main pump drives the sample to be detected in the quantitative structure to the quantitative structure. Before the test sample is driven to the analyzer by the quantitative component, it also includes:

The control part controls the first three-way solenoid valve to close the normally closed end of the second three-way solenoid valve and open the normally open end of the second three-way solenoid valve.

Compared with the prior art, the method for online real-time detection of biological samples provided by the present application controls the main pump and the feeding component through the control part, so that the main pump drives the sample to be detected in the culture vessel through the sampling pipe to the quantitative structure of the quantitative component. In order to quantify, and the sample to be tested quantified by the quantitative structure can be driven by the quantitative component to the analyzer, the analyzer receives the sample to be tested and analyzes the sample to be tested to obtain the analysis result, and the control part obtains the analysis result from the analyzer And control the feeding component to feed the culture vessel according to the analysis result.

Therefore, the beneficial effects of the biological sample online real-time detection method provided by the present application are:

First, the sampling procedure is simplified, the time from sampling to feeding is shortened, and the problem that the prior art cannot satisfy high-frequency and high-efficiency sampling because the sampling procedure is cumbersome and time-consuming;

Second, the single sampling amount can be precisely controlled, which solves the problem that the sampling amount cannot be accurate due to manual sampling in the prior art;

Fourth, the whole process is automated and integrated, which solves the problem of high labor costs caused by the need for a large number of manual participation in the entire monitoring process in the prior art.

Description of drawings

In order to illustrate the technical solutions in the embodiments of the present application more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only for the present application. In some embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without any creative effort.

Fig. 1 is the first schematic diagram of the biological sample online real-time detection system provided by the embodiment of this application;

FIG. 2 is a second schematic diagram of the online real-time detection system for biological samples provided by the embodiment of the present application.

Among them, each reference sign in the figure:

10. Culture container; 20. Sampling pipe fittings; 30. Quantitative component; 40. Sampling host; 50. Sample receiving component; 60. Analyzer; 70. Feeding component;

201, probe tube; 202, membrane tube; 203, rotary valve; 204, hose;

301, the first three-way solenoid valve; 302, the second three-way solenoid valve; 303, the disinfection container; 304, the air filter; 305, the first liquid detector; 306, the second liquid detector;

401, main pump; 402, solenoid valve;

501, drainage assembly; 502, overflow assembly; 503, water inlet assembly; 504, receiving tank; 505, sample transfer needle; 506, third liquid detector; 507, stirring device;

501a, a drainage pipe; 501b, a first drive pump; 501c, a first collection container;

502a, overflow pipeline; 502b, second driving pump;

503a, the water inlet pipe; 503b, the third driving pump; 503c, the pure water container;

601 - Display;

701, feeding pipeline; 702, feeding pump; 703, feeding container;

a, normally closed end; b, normally open end; c, public end.

Detailed ways

In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application clearer, the present application will be described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application.

It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

It is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inside", "outside", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, only for the convenience of describing the application and simplifying the description, rather than indicating or implying the indicated A device or element must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as a limitation of the present application.

In addition, the terms "first" and "second" are only used for descriptive purposes, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first" or "second" may expressly or implicitly include one or more of that feature. In the description of the present application, "plurality" means two or more, unless otherwise expressly and specifically defined.

The online real-time detection system and detection method for biological samples provided in the embodiments of the present application will now be described.

Referring to FIG. 1 , the system for online real-time detection of biological samples provided by the embodiments of the present application includes: a culture container 10 for accommodating a sample to be detected; a sampling tube 20 , the first end of which is used to extend into the sample to be detected Below the liquid level, the second end of the sampling tube 20 is connected to the quantitative component 30, and the sampling tube 20 is used to sample the sample to be detected from the culture container 10 to the quantitative component 30; the quantitative component 30 includes a quantitative structure, the first of the quantitative structure is The end is connected to the second end of the sampling tube 20, the second end of the quantitative structure is connected to the sampling host 40, and the quantitative structure is used to determine the amount of the sample to be detected sampled from the culture vessel 10 to the quantitative component 30; the sampling host 40 includes a main The pump 401, the main pump 401 is connected between the quantitative component 30 and the analyzer 60 through a pipeline, the main pump 401 is used to drive the sample to be detected from the culture vessel 10 to the quantitative component 30 and is used to determine the quantitative structure of the sample to be detected. Drive to the analyzer 60; the analyzer 60 is used to receive the sample to be tested and analyze the sample to obtain the analysis result; the feeding component 70 is connected to the feeding port of the culture container 10 through the pipeline; the control part, the main pump 401. Both the analyzer 60 and the feeding component 70 are signal-connected with the control part, and the control part is used for acquiring the analysis result and controlling the feeding component 70 to feed the culture vessel 10 according to the analysis result.

Compared with the prior art, the on-line real-time detection system for biological samples provided by the embodiments of the present application, through the combination of the control part and the quantitative component 30, determines the real-time detection of the sample to be detected from the culture container 10 to the quantitative component 30 through the quantitative structure. The main pump 401 drives the sample to be detected from the culture vessel 10 to the quantitative component 30 in real time, and drives the sample to be detected determined by the quantitative structure to the analyzer 60, and the analyzer 60 analyzes the sample to be detected in real time to obtain an analysis As a result, through the real-time control of the control system, the feeding component 70 can be controlled in real time to feed the culture vessel 10 according to the analysis result. which has the following

Therefore, the beneficial effects of the biological sample online real-time detection system provided by this application are:

First, the sampling procedure is simplified, the time from sampling to feeding is shortened, and the problem that the prior art cannot satisfy high-frequency and high-efficiency sampling because the sampling procedure is cumbersome and time-consuming;

Second, the single sampling amount can be precisely controlled, which solves the problem that the sampling amount cannot be accurate due to manual sampling in the prior art;

Fourth, the whole process is automated and integrated, which solves the problem of high labor costs caused by the need for a large number of manual participation in the entire monitoring process in the prior art.

In this embodiment of the present application, the control part may be independent of other components, or the control part may be integrated into the sampling host 40 , or the control part may be integrated into the analyzer 60 .

In this embodiment of the present application, the sampling host 40 and the analyzer 60 may be set independently, or the two may be integrated into one. When the sampling host 40 and the analyzer 60 are integrated, the control part is integrated into the integrated structure.

In one embodiment, the sampling tube 20 includes a probe tube 201 , a membrane tube 202 and a rotary valve 203 ; wherein, the first end of the probe tube 201 extends into the interior of the culture vessel 10 , and the second end of the probe tube 201 is connected by a hose 204 in the quantitative component 30. Wherein, the tube wall of the probe tube 201 and the culture container 10 are sealed by a sealing link, for example, a sealing ring can be used for sealing connection. Wherein, the hose 204 is preferably a Teflon tube, and the rotary valve 203 is arranged at one end of the hose 204 close to the probe tube 201. The rotary valve 203 is a rotary valve, and the rotary valve is used to control the on-off of the hose 204, and its type can be It can be selected arbitrarily, for example, the manual rotary valve 203 can be selected. Of course, in other embodiments, the setting of the rotary valve 203 can be eliminated.

The membrane tube 202 is connected to the first end of the probe tube 201, and the membrane tube 202 is used to extend below the liquid level of the sample to be detected. The membrane tube 202 is a microporous ceramic tube, wherein the diameter of the micropores is greater than or equal to 0.2 μm. Since the diameter of the micropores can be as small as 0.2 μm, while the diameter of general bacteria is about 0.5 μm, the bacteria can be effectively isolated and the medium can be filtered efficiently. Of course, the membrane tube 202 can be designed with other materials or other shapes.

In one embodiment, the quantitative component 30 further includes a first three-way solenoid valve 301, a second three-way solenoid valve 302, a sterilizing container 303, and an air filter 304; wherein the normally closed end a of the first three-way solenoid valve 301 is connected to At the second end of the sampling pipe 20, the normally open end b of the first three-way solenoid valve 301 is connected to the common end c of the second three-way solenoid valve 302 through a pipeline, and the common end c of the first three-way solenoid valve 301 is connected to the quantitative The first end of the structure; the normally closed end a of the second three-way solenoid valve 302 is connected to the disinfection container 303 through a pipeline, the disinfection container 303 is used to accommodate the disinfectant, and the normally open end b of the second three-way solenoid valve 302 is connected to the disinfection container 303 through a pipeline. The air filter 304; the first three-way solenoid valve 301 and the second three-way solenoid valve 302 are all signal-connected to the control part. The embodiment of the present application adopts a three-way solenoid valve, which can conveniently control the real-time operation of the detection system, and improve the real-time degree and accuracy of the control.

Wherein, the above-mentioned quantitative structure adopts a tube with a small inner diameter, and the quantitative is realized by matching a liquid detection device with a fixed length. In other alternative solutions, the quantification can be achieved by means of a fixed time or a fixed weight, which is a well-known technology in the art, and will not be repeated here.

The filter element of the air filter 304 is made of waterproof and breathable material with a micropore diameter of 0.2 μm, which can effectively isolate bacteria.

In one embodiment, the quantitative assembly 30 further includes a first liquid detector 305 and a second liquid detector 306 ; wherein the normally closed end a of the second three-way solenoid valve 302 is connected to the first liquid detector 305 through a pipeline. One port, the disinfection container 303 is connected to the second port of the first liquid detector 305 through a pipeline; the second end of the quantitative structure is connected to the first port of the second liquid detector 306, and the main pump 401 is connected to the second liquid detector 306 through a pipeline. The second port of the detector 306; the first liquid detector 305 and the second liquid detector 306 are both signal-connected to the control part.

In one embodiment, the sampling host 40 further includes a solenoid valve 402, the second end of the quantitative structure is connected to the normally closed end a of the solenoid valve through a pipeline, the main pump 401 is connected to the normally closed end a of the solenoid valve through a pipeline, the solenoid valve and Control section signal connections.

As shown in FIG. 2 , in one embodiment, the sampling host 40 includes at least two solenoid valves 402 , and at least two normally closed ends a of the at least two solenoid valves 402 are connected to the main pump 401 through pipelines; online real-time detection of biological samples The system includes at least two sampling tubes 20, at least two quantitative components 30 and at least two feeding components 70; at least two sampling tubes 20, at least two quantitative components 30 and at least two feeding components 70 are connected in one-to-one correspondence ; At least two second ends of at least two quantitative structures in at least two quantitative components 30 and at least two normally closed ends a of at least two solenoid valves 402 are connected in one-to-one correspondence.

In this embodiment, the sampling host 40 is provided with four channels, and each channel has a solenoid valve 402 for independently controlling the flow path. In the culture vessel 10, the other ends of the four channels are assembled to the pump tube of the main pump 401 through a plurality of three-way joints.

In one embodiment, the online real-time detection system for biological samples further includes a sample receiving assembly 50; the sample receiving assembly 50 includes a receiving cell 504 and a sample transfer needle 505 connected to the receiving cell 504, the receiving cell 504 is connected to the main pump 401, and the sample transfer needle 505 is connected to the analyzer 60 .

Wherein, in the embodiment of the present application, the sample receiving component 50 may be integrated into the analyzer 60 or independently designed outside the analyzer 60 . Preferably, in the present application, the sample receiving assembly 50 is integrated into the analyzer 60 .

In one embodiment, the sample receiving assembly 50 further includes at least one of a drainage assembly 501 , a water inlet assembly 503 , and an overflow assembly 502 . In this application, preferably, the sample receiving assembly 50 includes a drainage assembly 501 , a water inlet assembly 503 and an overflow assembly 502 .

The drainage assembly 501 includes a drainage pipe 501a, a first driving pump 501b and a first collection container 501c. The first end of the drainage pipe 501a is connected to the receiving tank 504, and the second end of the drainage pipe 501a is connected to the first collection container 501c. The first driving pump 501b is arranged on the drainage pipe 501a; the overflow assembly 502 includes an overflow pipe 502a, a second driving pump 502b and a second collecting container, the first end of the overflow pipe 502a is connected to the receiving tank 504, and the overflow pipe The second end of 502a is connected to the second collection container, and the second driving pump 502b is arranged on the overflow pipe 502a; the water inlet assembly 503 includes the water inlet pipe 503a, the third driving pump 503b and the pure water container 503c, and the pure water container 503c Used to accommodate pure water, the first end of the water inlet pipe 503a is connected to the receiving tank 504, the second end of the water inlet pipe 503a is connected to the pure water container 503c, and the third driving pump 503b is arranged on the water inlet pipe 503a; The drive pump 501b, the second drive pump 502b, and the third drive pump 503b are all signal-connected to the control section.

In the embodiment of the present application, the first collection container 501c and the second collection container are shared, of course, the two may also be provided independently.

In one embodiment, the sample receiving assembly 50 further includes a third liquid detector 506, the main pump 401 is connected to the first port of the third liquid detector 506 through a pipeline, and the sample receiving tank 504 is connected to the third liquid detector 506 through a pipeline The second port of the third liquid detector 506 is signally connected to the control part.

In one embodiment, the feeding assembly 70 includes a feeding pipeline 701, a feeding pump 702 and a feeding container 703, and the feeding container 703 is used for containing the feeding liquid; wherein, the first end of the feeding pipeline 701 is connected to the culture container 10, the second end of the feeding pipe 701 is used to extend below the liquid level of the feeding liquid; the feeding pump 702 is arranged on the feeding pipe 701, and the feeding pump 702 is signally connected to the control part.

In the embodiment of the present application, each feeding channel is equipped with a feeding pump 702. In other alternative solutions, the feeding pump 702 may not be configured, and the feeding is driven by the main pump 401, or a feeding channel is equipped with a feeding pump 702. Multiple feed pumps 702.

Another object of the present application is to provide a detection method corresponding to the above-mentioned biological sample online real-time detection system, comprising:

Start and run the main pump 401 through the control part, so that the main pump 401 drives the sample to be detected in the culture vessel 10 through the sampling tube 20 to the quantitative structure of the quantitative assembly 30 for quantitative determination;

The main pump 401 drives the sample to be detected quantified by the quantitative structure from the quantitative component 30 to the analyzer 60;

The analyzer 60 receives the sample to be tested and analyzes the sample to be tested to obtain an analysis result;

The control part obtains the analysis result from the analyzer 60 and controls the feeding component 70 to feed the culture vessel 10 according to the analysis result.

In one embodiment, before the main pump 401 drives the sample to be detected in the culture container 10 to the quantitative structure of the quantitative component 30 through the sampling tube 20 for quantitative measurement, the method further includes:

The control part controls the solenoid valve 402 to open the normally closed end a of the solenoid valve 402 and close the normally open end b of the solenoid valve 402;

The control part controls the second three-way solenoid valve 302 to open the normally closed end a of the second three-way solenoid valve 302 and close the normally open end b of the second three-way solenoid valve 302;

The main pump 401 drives the sterilizing liquid in the sterilizing container 303 to the quantitative structure through the second three-way solenoid valve 302 and the first three-way solenoid valve 301 for quantitative measurement;

The quantitative structure completes the quantification of the disinfectant, the first liquid detector 305 obtains the quantitative completion signal and sends it to the control part, and the control part controls the second three-way solenoid valve 302 according to the quantitative completion signal to close the normally closed second three-way solenoid valve 302. end a and open the normally open end b of the second three-way solenoid valve 302;

The main pump 401 drives the sterilizing solution determined by the quantitative structure to the sample receiving assembly 50 from the quantitative structure.

In one embodiment, after the main pump 401 drives the quantitative structure of the disinfectant to the sample receiving assembly 50 from the quantitative structure, the main pump 401 drives the sample to be detected in the culture container 10 to the quantitative assembly 30 through the sampling tube 20 In the Quantitative Structure of Quantitative before quantification, also include:

The receiving tank 504 of the sample receiving assembly 50 receives the disinfectant and overflows, and the third liquid detector 506 obtains the overflow signal and sends it to the control part, and the control part controls the second drive pump 502b according to the overflow signal. Drive to the second collection container, the control part controls the solenoid valve 402 according to the overflow signal to close the normally closed end a of the solenoid valve 402 and open the normally open end b of the solenoid valve 402;

The control part controls the first driving pump 501b to drive the disinfectant in the receiving tank 504 into the first collecting container 501c;

The control part controls the third driving pump 503b to drive the pure water in the pure water container 503c to the receiving tank 504, and the control part controls the second driving pump 502b to drive the pure water in the receiving tank 504 to the second collecting container.

In one embodiment, the control part controls the third driving pump 503b to drive the pure water in the pure water container 503c to the receiving tank 504, and the control part controls the second driving pump 502b to drive the pure water in the receiving tank 504 to the second After the collection container, before the main pump 401 drives the sample to be detected in the culture container 10 through the sampling tube 20 to the quantitative structure of the quantitative component 30 for quantitative measurement, the method further includes:

The control part controls the first three-way solenoid valve 301 to open the normally closed end a of the second three-way solenoid valve 302 and close the normally open end b of the second three-way solenoid valve 302 .

In one embodiment, after the main pump 401 drives the sample to be detected in the culture vessel 10 through the sampling tube 20 to the quantitative structure of the quantitative component 30 for quantification, the main pump 401 drives the sample to be detected quantified by the quantitative structure to the quantitative structure of the quantitative component 30 for quantification. Before the quantitative assembly 30 is driven to the analyzer 60, it also includes:

The control part controls the first three-way solenoid valve 301 to close the normally closed end a of the second three-way solenoid valve 302 and open the normally open end b of the second three-way solenoid valve 302 .

It should be noted that the sampling tube 20 needs to be subjected to high temperature sterilization before being loaded into the culture container 10 . After the sampling tube 20 is loaded into the culture container 10 , the sampling tube 20 may be sterilized in synchronization with the sterilization process of the culture container 10 . The swirl valve 203 at its tail is used for in-situ sterilization operation. In addition, all the pipelines of the feed pump 702 are also subjected to high temperature sterilization.

Taking a single channel as an example, the above-mentioned online real-time detection method of biological samples is specifically:

1. Disinfection, cleaning and emptying actions

After the power is turned on, the detection system first automatically performs disinfection, cleaning and emptying actions. The control part sends instructions according to the start signal to control to open the normally closed end a of the solenoid valve 402 corresponding to the channel and close the normally open end b, and at the same time control to open the second three-way The normally closed end a of the solenoid valve 302 closes the normally open end b. After that, the control part controls the main pump 401 to start to rotate counterclockwise, and drives the disinfectant in the disinfection container 303 to enter the quantitative structure of the quantitative assembly 30. The quantitative structure realizes the disinfection of the entering through a fixed-length tube with a certain inner diameter and the second liquid detector 306. The quantification of liquid, the default quantification is 1mL, of course, the quantification can be adjusted according to the situation.

When the quantification is completed, the control part controls to close the normally closed end a of the second three-way solenoid valve 302 and open its normally open end b, the main pump 401 continues to rotate, and the liquid tail of the disinfectant is connected to the atmosphere through the air filter 304. Under the continuous rotation of the main pump 401, the quantitatively extracted disinfectant is pumped into the receiving tank 504 in the analyzer 60, and the flow path near the main pump 401 is emptied at the same time.

When the disinfectant reaches the third liquid detector 506 inside the analyzer 60, it means that the receiving tank 504 overflows, and the second driving pump 502b inside the analyzer 60 starts to rotate continuously, filling the receiving tank 504 and overflowing out. The excess disinfectant is pumped into the second collection container.

When the third liquid detector 506 detects that there is liquid to no liquid, the second driving pump 502b stops rotating after working for a short period of time. At the same time, the control part controls to close the normally closed end a of the solenoid valve 402 and open the normally open end b thereof. At this time, the stirring device 507 inside the analyzer 60 starts stirring for a certain period of time, so as to perform stirring, disinfection and cleaning in the receiving tank 504 .

Then the control part controls the first driving pump 501b inside the analyzer 60 to discharge the disinfectant in the receiving tank 504 into the first collecting container 501c, and after the set time elapses, the control part controls and stops the rotation of the first driving pump 501b. The control part controls the third driving pump 503b to pump pure water into the receiving tank 504, and controls the second driving pump 502b to rotate to pump the pure water in the receiving tank 504 into the second collecting container. After the set time has elapsed, the control part controls and stops the third driving pump 503b, and at this time, the control part controls the second driving pump 502b to stop running after a short period of time. Subsequently, the control part controls the first driving pump 501b to start rotating to empty the receiving tank 504, and enters a standby state after completion.

2. Online sampling action

After the set time is reached, online sampling is performed. The control part controls to open the normally closed end a of the first three-way solenoid valve 301, and simultaneously opens the normally closed end a of the first three-way solenoid valve 301. At the same time, the control part starts the main pump 401 to make it When the operation is started, the sample to be detected in the culture vessel 10 is pumped into the quantitative structure of the quantitative component 30 through the membrane tube 202 for quantitative determination.

When the liquid head filtered through the sampling tube 20 reaches the second liquid detector 306 of the quantitative component 30, the accurate quantitative measurement of the sample is completed, and the default value is 1 mL. At this time, the control part controls to close the normally closed end a of the first three-way solenoid valve 301 and open the normally open end b of the first three-way solenoid valve 301 .

Under the continuous operation of the main pump 401 , the extracted sample to be detected is pumped into the receiving tank 504 inside the analyzer 60 . When the liquid head of the sample to be detected reaches the third liquid detector 506 in the analyzer 60, the second driving pump 502b on the overflow pipe 502a starts to run continuously. sample to prevent contamination from overflowing into the instrument. When the sample liquid tail in the pipeline is separated from the third liquid detector 506, the control part controls the main pump 401 to stop running, closes the normally closed port a of the solenoid valve 402 and stops the operation of the second driving pump 502b to complete the sampling operation Wait for the sample transfer needle 505 to transfer the sample into the receiving cell 504 .

3. Analysis action

After the online sampling is completed, the sample transfer needle 505 enters the receiving tank 504 for quantitative sampling, and moves the sample to the test position of the analyzer 60 for analysis and testing. After the analysis is completed, the test results can be displayed on the display 601 and printed out through the printer at the analyzer 60 end, and finally the entire system can be disinfected, cleaned and emptied.

4. Reverse control action

Before use, the user can set the expected control concentration value of the measured parameter, as well as input the concentration value of the feed solution and the initial liquid filling volume of the culture tank. When the sampling test results come out, the control part judges whether to feed the culture tank according to the expected control parameters set by the user. If feeding is required, the control part automatically and accurately calculates the amount of feeding according to the changes of various parameters of the feeding, and the analyzer 60 sends a signal to the control part, thereby controlling the feeding pump 702 to quantitatively adjust the feeding liquid. The culture container 10 is added to automatically realize quantitative control of the measured parameters in the culture container 10 .

Compared with the prior art, in the online real-time detection method of biological samples provided by the present application, the main pump 401 and the feeding component 70 are controlled by the control part, so that the main pump 401 drives the sample to be detected in the culture vessel 10 through the sampling pipe 20 to The quantitative structure of the quantitative component 30 is used for quantification, and the sample to be detected quantified by the quantitative structure can be driven from the quantitative component 30 to the analyzer 60, and the analyzer 60 receives the sample to be tested and analyzes the sample to be tested to obtain an analysis result. , the control part obtains the analysis result from the analyzer 60 and controls the feeding component 70 to feed the culture vessel 10 according to the analysis result.

The above descriptions are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection of the present application. within the range.

Claims (15)

1. a biological sample online real-time detection system, is characterized in that, comprises: a culture container (10) for containing the sample to be tested; A sampling pipe (20), the first end of the sampling pipe (20) is used to extend below the liquid level of the sample to be detected, and the second end of the sampling pipe (20) is connected to the quantitative component (30), The sampling tube (20) is used for sampling the sample to be detected from the culture container (10) to the quantitative component (30); The quantitative component (30) includes a quantitative structure, the first end of the quantitative structure is connected to the second end of the sampling pipe (20), and the second end of the quantitative structure is connected to the sampling host (40), The quantitative structure is used to determine the amount of the sample to be detected sampled from the culture vessel (10) to the quantitative component (30); The sampling host (40) includes a main pump (401), the main pump (401) is connected between the quantitative component (30) and the analyzer (60) through a pipeline, and the main pump (401) uses for driving the sample to be detected from the culture vessel (10) to the quantitative component (30) and for driving the sample to be detected determined by the quantitative structure to the analyzer (60); the analyzer (60), configured to receive the sample to be detected and analyze the sample to be detected to obtain an analysis result; a feeding component (70), connected to the feeding port of the culture vessel (10) through a pipeline; A control part, the main pump (401), the analyzer (60) and the feeding component (70) are all signally connected with the control part, and the control part is used for acquiring the analysis result and according to the The analysis result controls the feeding component (70) to feed the culture vessel (10). 2. biological sample online real-time detection system as claimed in claim 1, is characterized in that: The biological sample online real-time detection system further includes a sample receiving component (50); The sample receiving assembly (50) includes a receiving pool (504) and a sample transfer needle (505) connected to the receiving pool (504), and the receiving pool (504) is connected to the main pump (401), so The transfer needle (505) is connected to the analyzer (60). 3. biological sample online real-time detection system as claimed in claim 1 or 2, is characterized in that: The quantitative assembly (30) further comprises a first three-way solenoid valve (301), a second three-way solenoid valve (302), a sterilizing container (303), and an air filter (304); wherein, The normally closed end (a) of the first three-way solenoid valve (301) is connected to the second end of the sampling pipe (20), and the normally open end (b) of the first three-way solenoid valve (301) passes through The pipeline is connected to the common end (c) of the second three-way solenoid valve (302), and the common end (c) of the first three-way solenoid valve (301) is connected to the first end of the quantitative structure; The normally closed end (a) of the second three-way solenoid valve (302) is connected to the sterilization container (303) through a pipeline, and the sterilization container (303) is used for accommodating the disinfectant, and the second three-way solenoid valve (302) The normally open end (b) of the valve (302) is connected to the air filter (304) by a pipe; Both the first three-way solenoid valve (301) and the second three-way solenoid valve (302) are signally connected to the control part. 4. biological sample online real-time detection system as claimed in claim 3 is characterized in that: The quantitative assembly (30) further includes a first liquid detector (305) and a second liquid detector (306); wherein, The normally closed end (a) of the second three-way solenoid valve (302) is connected to the first port of the first liquid detector (305) through a pipeline, and the disinfection container (303) is connected to the the second port of the first liquid detector (305); The second end of the quantitative structure is connected to the first port of the second liquid detector (306), and the main pump (401) is connected to the second port of the second liquid detector (306) through a pipeline ; Both the first liquid detector (305) and the second liquid detector (306) are signally connected to the control part. 5. biological sample online real-time detection system as claimed in claim 1 or 2, is characterized in that: The sampling host (40) further comprises a solenoid valve (402), the second end of the quantitative structure is connected to the normally closed end (a) of the solenoid valve through a pipeline, and the main pump (401) is connected to the solenoid valve through a pipeline. The normally closed end (a) of the solenoid valve (402), the solenoid valve (402) is signally connected to the control part. 6. biological sample online real-time detection system as claimed in claim 5 is characterized in that: The sampling host (40) includes at least two solenoid valves (402), and at least two normally closed ends (a) of the at least two solenoid valves (402) are connected to the main pump (401) through pipes ); The biological sample online real-time detection system comprises at least two sampling tubes (20), at least two quantitative components (30) and at least two feeding components (70); at least two sampling tubes (20), At least two quantitative components (30) and at least two feeding components (70) are connected in a one-to-one correspondence; at least two second ends of at least two of the quantitative structures in the at least two quantitative components (30) and at least two normally closed ends (a) of at least two of the solenoid valves (402) in a one-to-one correspondence. 7. biological sample online real-time detection system as claimed in claim 2, is characterized in that: The sample receiving assembly (50) further includes at least one of a drainage assembly (501), a water inlet assembly (503), and an overflow assembly (502); wherein, The drainage assembly (501) includes a drainage pipe (501a), a first driving pump (501b) and a first collection container (501c), and the first end of the drainage pipe (501a) is connected to the receiving tank (504) , the second end of the drainage pipe (501a) is connected to the first collection container (501c), and the first driving pump (501b) is arranged on the drainage pipe (501a); The overflow assembly (502) includes an overflow pipe (502a), a second driving pump (502b) and a second collection container, and the first end of the overflow pipe (502a) is connected to the receiving tank (504) , the second end of the overflow pipe (502a) is connected to the second collection container, and the second driving pump (502b) is arranged on the overflow pipe (502a); The water inlet assembly (503) includes a water inlet pipe (503a), a third driving pump (503b) and a pure water container (503c), the pure water container (503c) is used for containing pure water, and the water inlet pipe The first end of (503a) is connected to the receiving tank (504), the second end of the water inlet pipe (503a) is connected to the pure water container (503c), and the third driving pump (503b) is provided on the water inlet pipe (503a); The first driving pump (501b), the second driving pump (502b) and the third driving pump (503b) are all signal-connected to the control part. 8. biological sample online real-time detection system as claimed in claim 7, is characterized in that: The sample receiving assembly (50) further includes a third liquid detector (506), the main pump (401) is connected to the first port of the third liquid detector (506) through a pipeline, and the sample receiving tank (504) is connected to the second port of the third liquid detector (506) through a pipeline, and the third liquid detector (506) is signally connected to the control part. 9. biological sample online real-time detection system as claimed in claim 1 or 2, is characterized in that: The sampling pipe (20) includes a probe pipe (201), a membrane pipe (202) and a rotary valve (203); wherein, The first end of the probe tube (201) extends into the interior of the culture container (10), and the second end of the probe tube (201) is connected to the quantitative assembly (30) through a hose (204); The rotary valve (203) is arranged at one end of the hose (204) close to the probe tube (201); the membrane tube (202) is connected to the first end of the probe tube (201), the The membrane tube (202) is used to extend below the liquid level of the sample to be detected. 10. The biological sample online real-time detection system according to claim 1 or 2, characterized in that: The feeding component (70) includes a feeding pipeline (701), a feeding pump (702) and a feeding container (703), and the feeding container (703) is used for containing feeding liquid; wherein, The first end of the feeding pipe (701) is connected to the feeding port of the culture container (10), and the second end of the feeding pipe (701) is used to extend into the liquid level of the feeding liquid Below; the feeding pump (702) is arranged on the feeding pipeline (701), and the feeding pump (702) is signally connected with the control part. 11. A detection method of the biological sample online real-time detection system according to any one of claims 1-10, wherein: The main pump (401) is started and operated by the control part, so that the main pump (401) drives the sample to be detected in the culture vessel (10) through the sampling tube (20) to the quantitative structure of the quantitative assembly (30) for quantitative measurement ; The main pump (401) drives the sample to be detected quantified by the quantitative structure to the analyzer (60) from the quantitative component (30); The analyzer (60) receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result; The control part obtains the analysis result from the analyzer (60) and controls the feeding component (70) to feed the culture vessel (10) according to the analysis result. 12. The method for online real-time detection of biological samples as claimed in claim 11, wherein: Before the main pump (401) drives the sample to be detected in the culture vessel (10) through the sampling tube (20) into the quantitative structure of the quantitative assembly (30) for quantitative measurement ,Also includes: the control part controls the solenoid valve (402) to open the normally closed end (a) of the solenoid valve (402) and close the normally open end (b) of the solenoid valve (402); The control part controls the second three-way solenoid valve (302) to open the normally closed end (a) of the second three-way solenoid valve (302) and close the normally open end of the second three-way solenoid valve (302) (b); The main pump (401) drives the disinfectant in the disinfection container (303) into the quantitative structure through the second three-way solenoid valve (302) and the first three-way solenoid valve (301) for quantitative determination; The quantitative structure completes the quantification of the disinfectant, and the first liquid detector (305) acquires a quantitative completion signal and sends it to the control part, and the control part controls the second three-way solenoid according to the quantitative completion signal a valve (302) to close the normally closed end (a) of the second three-way solenoid valve (302) and open the normally open end (b) of the second three-way solenoid valve (302); The main pump (401) drives the sterilizing solution quantified by the quantitative structure to the sample receiving assembly (50) from the quantitative structure. 13. The method for online real-time detection of biological samples as claimed in claim 12, wherein: After the main pump (401) drives the sterilizing solution determined by the quantitative structure to the sample receiving assembly (50) from the quantitative structure, the culture container (10) is driven by the main pump (401). Before the sample to be detected in ) is driven into the quantitative structure of the quantitative component (30) by the sampling tube (20) for quantitative measurement, it also includes: The receiving tank (504) of the sample receiving assembly (50) receives the disinfectant and overflows, and the third liquid detector (506) obtains an overflow signal and sends it to the control part, and the control part according to the The overflow signal controls the second driving pump (502b) to drive the disinfectant in the overflow part to the second collection container, and the control part controls the solenoid valve (402) according to the overflow signal to close the solenoid valve ( 402) the normally closed end (a) and open the normally open end (b) of the solenoid valve (402); The control part controls the first driving pump (501b) to drive the disinfectant in the receiving tank (504) into the first collection container (501c); The control part controls the third drive pump (503b) to drive the pure water in the pure water container (503c) to the receiving tank (504), and the control part controls the second drive pump (502b) to drive the The pure water in the receiving tank (504) is driven into the second collection container. 14. The method for online real-time detection of biological samples as claimed in claim 13, wherein: The control part controls the third drive pump (503b) to drive the pure water in the pure water container (503c) into the receiving tank (504), and the control part controls the second drive pump (502b) After driving the pure water in the receiving tank (504) into the second collection container, the main pump (401) passes the sample to be detected in the culture container (10) through Before the sampling tube (20) is driven into the quantitative structure of the quantitative component (30) for quantitative measurement, it further includes: The control part controls the first three-way solenoid valve (301) to open the normally closed end (a) of the second three-way solenoid valve (302) and close the second three-way solenoid valve (302) Normally open (b). 15. The method for online real-time detection of biological samples as claimed in claim 14, wherein: After the main pump (401) drives the sample to be detected in the culture container (10) through the sampling tube (20) to the quantitative structure of the quantitative component (30) for quantitative measurement, the main pump (401) will Before the sample to be detected quantified by the quantitative structure is driven by the quantitative component (30) to the analyzer (60), it further includes: The control part controls the first three-way solenoid valve (301) to close the normally closed end (a) of the second three-way solenoid valve (302) and open the second three-way solenoid valve (302) Normally open (b).

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