CN113397156A - Dual Pickering emulsion and preparation method thereof - Google Patents
Dual Pickering emulsion and preparation method thereof Download PDFInfo
- Publication number
- CN113397156A CN113397156A CN202110636030.1A CN202110636030A CN113397156A CN 113397156 A CN113397156 A CN 113397156A CN 202110636030 A CN202110636030 A CN 202110636030A CN 113397156 A CN113397156 A CN 113397156A
- Authority
- CN
- China
- Prior art keywords
- solution
- pickering emulsion
- double
- chitosan
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 230000009977 dual effect Effects 0.000 title description 28
- 238000001484 Pickering emulsion method Methods 0.000 title description 4
- 239000000839 emulsion Substances 0.000 claims abstract description 129
- 239000002245 particle Substances 0.000 claims abstract description 68
- 239000002131 composite material Substances 0.000 claims abstract description 56
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 47
- 244000068988 Glycine max Species 0.000 claims abstract description 46
- 229920001661 Chitosan Polymers 0.000 claims abstract description 45
- 239000000243 solution Substances 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 37
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000000265 homogenisation Methods 0.000 claims abstract description 7
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 6
- 239000012498 ultrapure water Substances 0.000 claims abstract description 6
- 239000006185 dispersion Substances 0.000 claims abstract description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 5
- 238000004945 emulsification Methods 0.000 claims description 19
- 108010073771 Soybean Proteins Proteins 0.000 claims description 13
- 238000010008 shearing Methods 0.000 claims description 13
- 235000019710 soybean protein Nutrition 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000003921 oil Substances 0.000 claims description 8
- 235000019198 oils Nutrition 0.000 claims description 7
- 239000004519 grease Substances 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 239000002285 corn oil Substances 0.000 claims description 4
- 235000005687 corn oil Nutrition 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 238000002525 ultrasonication Methods 0.000 claims 1
- 239000000047 product Substances 0.000 abstract description 10
- 239000000084 colloidal system Substances 0.000 abstract description 6
- 230000001079 digestive effect Effects 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 235000013305 food Nutrition 0.000 description 17
- 239000003995 emulsifying agent Substances 0.000 description 11
- 230000008569 process Effects 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 9
- 239000012071 phase Substances 0.000 description 9
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 description 8
- 230000029087 digestion Effects 0.000 description 8
- 239000004094 surface-active agent Substances 0.000 description 8
- 239000003814 drug Substances 0.000 description 6
- 230000002496 gastric effect Effects 0.000 description 6
- 239000002537 cosmetic Substances 0.000 description 5
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 4
- 239000008346 aqueous phase Substances 0.000 description 4
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 4
- 235000005487 catechin Nutrition 0.000 description 4
- 229950001002 cianidanol Drugs 0.000 description 4
- 235000012754 curcumin Nutrition 0.000 description 4
- 229940109262 curcumin Drugs 0.000 description 4
- 239000004148 curcumin Substances 0.000 description 4
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- -1 lecithin modified kaolinite Chemical class 0.000 description 3
- 239000004005 microsphere Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 206010034972 Photosensitivity reaction Diseases 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical class O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 229940067606 lecithin Drugs 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- SHXOKQKTZJXHHR-UHFFFAOYSA-N n,n-diethyl-5-iminobenzo[a]phenoxazin-9-amine;hydrochloride Chemical compound [Cl-].C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=[NH2+])C2=C1 SHXOKQKTZJXHHR-UHFFFAOYSA-N 0.000 description 2
- VOFUROIFQGPCGE-UHFFFAOYSA-N nile red Chemical compound C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=O)C2=C1 VOFUROIFQGPCGE-UHFFFAOYSA-N 0.000 description 2
- 230000036211 photosensitivity Effects 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009102 absorption Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 239000011246 composite particle Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000006081 fluorescent whitening agent Substances 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/066—Multiple emulsions, e.g. water-in-oil-in-water
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
- A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Birds (AREA)
- Dispersion Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dermatology (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Colloid Chemistry (AREA)
Abstract
本发明公开了一种双重Pickering乳液及其制备方法。该方法采用超纯水将大豆水不溶性聚集体配成肽分散液，超声得到大豆水不溶性聚集体溶液；采用醋酸水溶液将壳聚糖配成溶液，获得壳聚糖溶液；将壳聚糖溶液缓慢滴于不断搅拌的大豆水不溶性聚集体溶液当中，并进行剪切均质获得复合胶体体系；调节包含复合胶体颗粒体系的pH为3～5，再与油脂混合，剪切乳化，得产物。本发明的双重乳液具有较高的稳定性，能够储藏长达四个月以上的时间不发生破乳漏油现象，乳液的双重结构能够保持一个月以上的时间，且流变和消化性能良好，有效的提高大豆蛋白酶解产物的绿色可持续化应用。
The invention discloses a double Pickering emulsion and a preparation method thereof. The method adopts ultrapure water to formulate soybean water-insoluble aggregates into a peptide dispersion, and ultrasonically obtains a soybean water-insoluble aggregate solution; uses acetic acid aqueous solution to formulate chitosan into a solution to obtain a chitosan solution; the chitosan solution is slowly Drop into the continuously stirred soybean water-insoluble aggregate solution, and perform shear homogenization to obtain a composite colloid system; adjust the pH of the system containing the composite colloid particles to 3-5, then mix with oil, shear and emulsify to obtain a product. The double emulsion of the invention has high stability, can be stored for more than four months without demulsification and oil leakage, the double structure of the emulsion can be maintained for more than one month, and has good rheological and digestive properties. Effectively improve the green and sustainable application of soybean protease hydrolysate.
Description
Technical Field
The invention relates to a double Pickering emulsion, in particular to a double Pickering emulsion with stable soybean water-insoluble aggregates and chitosan composite colloidal particles and a preparation method thereof.
Background
The double emulsion is a relatively complex emulsion system in an emulsion system, can simultaneously exist two emulsion types of W/O and O/W, and is widely applied to multiple fields of food industry, medicines, cosmetics and the like by virtue of a unique internal structure of the double emulsion. The two main types of double emulsions are water-in-oil-in-water (W/O/W) and oil-in-water-in-oil (O/W/O).
The stabilization of double emulsion systems in the conventional manner usually requires that two or more surfactants are achieved by a two-step emulsification process. However, this approach is more complicated in procedure and requires additional steps or ion concentration to increase the osmotic pressure of the internal aqueous phase to prevent the external permeation of the internal aqueous phase. To overcome these drawbacks, the stabilization of double emulsions by means of a one-step emulsification process has received extensive attention. The approaches for stabilizing double emulsion by one-step emulsification method mainly include microfluidic method, block copolymer method and Pickering emulsion method. The Pickering emulsion method utilizes the colloid particles which can be adsorbed to an oil-water interface to form a firm barrier, inhibits the aggregation of internal liquid drops so as to reduce the loss of an internal water phase, can improve the overall stability of a double-emulsion system, and is more nontoxic and environment-friendly compared with a surfactant. To date, the preparation of stable dual Pickering emulsions from silica particles, graphene oxide particles, and polymer particle bound surfactants by a two-step process has been investigated. However, of these colloidal particles, the use of edible colloidal particles to stabilize double emulsions in a single step has been rarely reported. Obviously, emulsification by fully edible colloidal particles under single high shear/high pressure action is a promising efficient and safe dual Pickering emulsion production method, and its potential application advantages have been the research focus in the food industry.
Chinese patent application 2019106160134 discloses a method for preparing W/O/W dual Pickering emulsion by using modified kaolinite as emulsifier and realizing the synergistic encapsulation of catechin and curcumin. The W1/O emulsion is obtained under the condition of shearing emulsification by using emulsified oil phase (O) of medium chain triglyceride dissolved with curcumin, emulsified water phase (W1) of deionized water dissolved with catechin and using lecithin modified kaolinite with a contact angle of 130 degrees as an emulsifier. Adding deionized water dispersed with lecithin modified kaolinite with a contact angle of 88 degrees into the obtained W1/O emulsion to obtain a W2 phase, and obtaining the W1/O/W2 double Pickering emulsion in which catechin and curcumin are encapsulated in an O phase and a W1 phase simultaneously under a shearing emulsification condition. The synergistic encapsulation efficiency of the double emulsion on oil-soluble curcumin and water-soluble catechin reaches 98 percent. However, the particle emulsifier in the technology is modified kaolinite, and is a non-food grade particle emulsifier, so that the application in the food field is limited; in addition, the technology adopts a two-step method to prepare the W1/O/W2 dual Pickering emulsion, and has complex preparation method and high cost.
Disclosure of Invention
Aiming at the defects that the food-grade double Pickering emulsion is difficult to develop in the prior art and a complex two-step preparation process is adopted, the invention provides the double Pickering emulsion which can be used in the field of food and has low preparation cost and stable soybean water-insoluble aggregate-chitosan composite colloidal particles without containing a surfactant and a preparation method thereof.
Compared with the prior art, the invention mainly solves the problems that only food-grade particle emulsifier is used, the method is different from the conventional two-step emulsification method, the double Pickering emulsion is prepared by the one-step emulsification method, the raw materials are natural and safe, and the preparation method is simple. The granular emulsifier used in the invention is edible soybean water-insoluble aggregate-chitosan composite colloidal particles, and is convenient to apply in food.
For the preparation of food-grade double Pickering emulsion, the main obstacle is to find a functional and food industry allowed particle system, and the prior art is complicated to prepare by a two-step method. The soybean protein is widely applied to the food industry, and the product obtained by enzymolysis of the soybean protein also has the beneficial functional health-care characteristic for human bodies. Meanwhile, insoluble aggregates SWIA (Soy water-insoluble aggregates) generated in the enzymolysis process of the soybean protein also contain a large amount of essential amino acids with uniform components, have excellent load bearing capacity and can be widely applied to the food industry as a potential functional food ingredient. Therefore, the water-insoluble aggregate after the enzymolysis of the soybean protein is used as the base material to stabilize the double Pickering emulsion, so that the green sustainable application of the soybean protein enzymolysis product can be further improved. Chitosan CS is an alkaline cationic polysaccharide, obtained from chitin by alkaline deacetylation. It has the characteristics of rich sources, good biocompatibility, biodegradability, bacteriostasis and the like, and is widely applied to various fields.
The invention develops food-grade composite colloidal particles, and further realizes the one-step preparation of the double Pickering emulsion. In the prior art, non-food-grade inorganic or polymer particles are combined with a surfactant to serve as an emulsifier of a double Pickering emulsion, and the preparation method is a two-step preparation method and is complicated in preparation process; the invention utilizes soybean protein enzymolysis by-products, namely soybean water-insoluble aggregates and chitosan to form composite colloidal particles, and the prepared composite colloidal particles can obtain stable double Pickering emulsion by a one-step emulsification method, thereby effectively improving the green sustainable application of soybean protein enzymolysis products, and having good application prospect in the fields of cosmetics, medicines, foods and health care products, including other fields. At present, no report is found on a method for preparing the double Pickering emulsion by using a food-grade colloidal particle one-step emulsification method in China.
The invention prepares the composite colloidal particles with stable structure by compounding the soybean water-insoluble aggregate SWIA and the chitosan CS, and prepares the stable double Pickering emulsion by using the composite colloidal particles as the emulsifier and utilizing a one-step homogenization method. In the invention, the double Pickering emulsion with high stability can be obtained without any surfactant and complicated procedures, and can be stored for a long time, so that the green sustainable application of the soybean protein enzymolysis product is effectively improved, and a convenient way is provided for preparing the multiple emulsions.
The purpose of the invention is realized by the following technical scheme:
the preparation method of the soybean water-insoluble aggregate-chitosan composite colloidal particle stable dual Pickering emulsion is characterized by comprising the following steps of:
preparing a soybean water-insoluble aggregate into a peptide dispersion liquid by adopting ultrapure water, and performing ultrasonic treatment to obtain a soybean water-insoluble aggregate solution; preparing chitosan into a solution by adopting an acetic acid aqueous solution to obtain a chitosan solution:
dripping the chitosan solution into the soybean water-insoluble aggregate solution which is continuously stirred, and shearing and homogenizing to obtain a system containing composite colloidal particles;
and adjusting the pH value of the composite colloidal particle system to 3-5, mixing with grease, shearing and emulsifying to obtain the double Pickering emulsion with stable soybean water-insoluble aggregate-chitosan composite colloidal particles.
In order to further achieve the purpose of the invention, preferably, the soybean water-insoluble aggregate SWIA is powder obtained by performing enzymolysis on soybean protein isolate for 12-24 hours by Protamex, and then performing freeze drying on a precipitate obtained after the enzymolysis liquid is centrifuged; the enzymatic hydrolysis condition of Protamex is set to be pH5.0-8.0, the temperature is 50 ℃, and the enzyme adding amount per gram of substrate is 000.5-0.01 ml according to the weight of the substrate. This method is commonly used in the art. In the examples, the enzyme hydrolysis was carried out for 20 hours under conditions of pH6.0 and 50 ℃ at an enzyme addition rate of 000.8 ml per gram of substrate, and the soybean water-insoluble aggregates SWIA obtained under other process conditions within the above-defined range were not substantially different.
Preferably, the mass concentration of the acetic acid aqueous solution is 1-3%.
Preferably, the dosage of the chitosan in the solution prepared by the chitosan with the acetic acid aqueous solution is 0.5-1% of the mass of the acetic acid solution;
the amount of the soybean water-insoluble aggregates in the peptide dispersion prepared from the soybean water-insoluble aggregates by adopting ultrapure water is 0.2-0.5% of the mass of water;
the volume ratio of the soybean water-insoluble aggregate solution to the chitosan solution in the continuously stirred soybean water-insoluble aggregate solution is 1: 1-2.5: 1.
Preferably, the ultrasonic power is 180W-200W, the intermittent time is 1-3 s, and the total time is 5-10 min. The ultrasonic power of the ultrasonic selection in the embodiment is 200W, the intermittent time is 2s, the total time is 8min, and the purpose of the invention can be realized by the treatment under the ultrasonic process condition.
Preferably, the rotation speed of the shearing homogenization is 15000rpm to 20000rpm, and the shearing homogenization time is 1 min to 2 min.
Preferably, the oil is corn oil.
Preferably, after the pH is adjusted to 3-5, the volume ratio of the composite colloidal particle system to the grease is 1: 1-3: 1.
Preferably, the rotation speed of the shearing emulsification is 8000 rpm-12000 rpm, and the time of the shearing emulsification is 30 s-80 s.
A soybean water-insoluble aggregate-chitosan composite colloidal particle stable double Pickering emulsion is prepared by the preparation method.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the invention utilizes the soybean water-insoluble aggregate-chitosan composite colloidal particles to stabilize the double Pickering emulsion by a one-step method for the first time, does not need more complicated procedures and does not add any surfactant.
(2) The double Pickering emulsion with stable soybean water-insoluble aggregate-chitosan composite colloidal particles has higher emulsification effect and emulsification stability, and can not be demulsified and leaked after being stored for more than four months.
(3) The soybean water-insoluble aggregate-chitosan composite colloidal particles for stabilizing the double Pickering emulsion are all natural substances, and the green sustainable application of soybean protein enzymolysis products is effectively improved, so that the soybean protein enzymolysis emulsion is healthy and nontoxic.
(4) The stable dual Pickering emulsion of the soybean water-insoluble aggregate composite colloidal particles has good rheological and digestive properties, and has good application prospects in the fields of cosmetics, medicines, foods and health products, including other fields.
Drawings
FIG. 1 is an appearance diagram of a double Pickering emulsion stabilized by freshly made SWIA-CS composite colloidal particles in an example; the double Pickering emulsion with stable composite colloidal particles of 0.25 percent SWIA-0.125 percent CS, 0.25 percent SWIA-0.25 percent CS and 0.25 percent SWIA-0.5 percent CS are sequentially arranged from left to right;
FIG. 2 is an appearance chart of a double Pickering emulsion stabilized by SWIA-CS composite colloidal particles in example 1 after being left for four months; the double Pickering emulsion with stable composite colloidal particles of 0.25 percent SWIA-0.125 percent CS, 0.25 percent SWIA-0.25 percent CS and 0.25 percent SWIA-0.5 percent CS are sequentially arranged from left to right;
FIG. 3 is a graph of the appearance of the 0.125%, 0.25% and 0.5% CS stabilized Pickering emulsions prepared in comparative example 1;
FIG. 4 is a graph of the appearance of the CS stabilized Pickering emulsions of comparative example 1 at 0.125%, 0.25%, and 0.5% after 24 hours of standing;
FIG. 5 is a microscopic image of a SWIA-CS composite colloidal particle-stabilized dual Pickering emulsion freshly prepared and left for 15 days in the examples; the double Pickering emulsion is stable with composite colloid particles of 0.25 percent SWIA-0.125 percent CS, 0.25 percent SWIA-0.25 percent CS and 0.25 percent SWIA-0.5 percent CS from top to bottom;
FIG. 6 is a microscopic image of new 0.125%, 0.25% and 0.5% CS-stabilized Pickering emulsions of comparative example 1;
FIG. 7 is a laser confocal map of the double Pickering emulsion stabilized by SWIA-CS composite colloidal particles of different CS mass concentrations in example 2; from left to right, the stable double Pickering emulsion of the composite colloidal particles of 0.25 percent SWIA-0.125 percent CS and 0.25 percent SWIA-0.25 percent CS are respectively arranged;
FIGS. 8 to 10 are respectively stress scan graphs of the composite colloidal particle stabilized dual Pickering emulsion of 0.25% SWIA-0.125% CS, 0.25% SWIA-0.25% CS, and 0.25% SWIA-0.5% CS in example 1; g 'represents an elastic modulus, and G' represents a viscous modulus;
FIGS. 11 and 12 are graphs of the changes of the stable dual Pickering emulsions of the composite colloidal particles 0.25% SWIA to 0.25% CS and 0.25% SWIA to 0.5% CS in the gastrointestinal digestion process in the examples, respectively.
Detailed Description
For a better understanding of the present invention, the present invention is further described below with reference to the accompanying drawings and examples, but the embodiments of the present invention are not limited thereto.
Comparative example 1
The preparation method of the chitosan stable dual Pickering emulsion comprises the following steps:
(1) accurately weighing 0.0125g, 0.025g and 0.05g of chitosan CS, and dissolving in 10mL of acetic acid solution with the mass concentration of 1% to obtain CS solutions with the mass concentrations of 0.125%, 0.25% and 0.5%, respectively;
(2) and (3) mixing the solution obtained in the step (1) with 3mL of corn oil, and performing shearing emulsification at the rotating speed of 15000rpm/min for 2min to obtain the Pickering emulsion with stable chitosan.
Examples 1 to 3
A preparation method of a soybean water-insoluble aggregate-chitosan one-step stable dual Pickering emulsion comprises the following steps:
(1) accurately weighing 0.05g of soybean water insoluble aggregate SWIA (obtained by enzymolysis of soybean protein) generated in the soybean enzymolysis process, dissolving the soybean water insoluble aggregate SWIA in 10mL of ultrapure water, performing ultrasonic treatment after overnight hydration to obtain a SWIA solution with the mass concentration of 0.5%;
(2) accurately weighing 0.1g of chitosan CS, and dissolving in 10mL of acetic acid solution with the mass concentration of 1% to obtain a CS solution with the mass concentration of 1%;
(3) slowly dripping the CS solution obtained in the step (2) into the SWIA solution obtained in the step (1), and shearing and homogenizing to obtain composite colloidal particles with the mass concentration of 0.25-0.5% of SWIA;
(4) and (3) mixing the solution obtained in the step (3) with 3mL of corn oil, and performing shearing emulsification at the rotating speed of 15000rpm/min for 2min to obtain the soybean water-insoluble aggregate-chitosan stable dual Pickering emulsion.
In this example, a gradient test was performed on the mass concentration of CS in step (2), and the mass concentrations of CS prepared were 0.125%, 0.25%, and 0.5%, respectively, to obtain dual Pickering emulsions with stable SWIA-CS composite colloidal particles of different mass concentrations of CS, specifically, dual Pickering emulsions with stable SWIA-0.125% of SWIA, 0.25% of SWIA-0.25% of CS, and 0.25% of SWIA-0.5% of CS composite colloidal particles, respectively.
As can be seen from the examples, compared with the common double Pickering emulsion in the prior art, the product obtained by the invention has the advantages that the particle emulsifier is a natural and safe food raw material and is prepared by a one-step emulsification method.
The obtained double emulsion structure is proved by microscope observation; the soybean water-insoluble aggregate-chitosan stable double Pickering emulsion product is characterized by the unchanged structure of the double emulsion through microscopic observation in the storage process and the combination of appearance stability; the double Pickering emulsion is W/O/W type as proved by the detection result of a laser confocal microscope by carrying out fluorescent dyeing on particles and grease.
The storage stability of the dual Pickering emulsions was characterized by observing their state after various periods of time. FIG. 1 is an appearance diagram of a double Pickering emulsion stabilized by freshly made SWIA-CS composite colloidal particles in an example; the double Pickering emulsion with stable composite colloidal particles of 0.25 percent SWIA-0.125 percent CS, 0.25 percent SWIA-0.25 percent CS and 0.25 percent SWIA-0.5 percent CS are sequentially arranged from left to right; FIG. 2 is an appearance chart of a double Pickering emulsion stabilized by SWIA-CS composite colloidal particles in example 1 after being left for four months; the double Pickering emulsion with stable composite colloidal particles of 0.25 percent SWIA-0.125 percent CS, 0.25 percent SWIA-0.25 percent CS and 0.25 percent SWIA-0.5 percent CS are sequentially arranged from left to right; FIG. 5 is a microscopic image of a SWIA-CS composite colloidal particle-stabilized dual Pickering emulsion freshly prepared and left for 15 days in the examples; the stable double Pickering emulsion is composed of composite colloid particles of 0.25 percent SWIA-0.125 percent CS, 0.25 percent SWIA-0.25 percent CS and 0.25 percent SWIA-0.5 percent CS from top to bottom. As can be seen from FIGS. 1 and 2, the dual Pickering emulsions stabilized by the composite colloidal particles of 0.25% SWIA-0.125% CS, 0.25% SWIA-0.25% CS, and 0.25% SWIA-0.5% CS, respectively, were stable for up to 4 months when stored without breaking emulsions. As can be seen from FIG. 5, the double emulsion structure of the emulsion remained and was stable after 15 days of storage.
FIG. 3 is a graph of the appearance of the 0.125%, 0.25% and 0.5% CS stabilized Pickering emulsions prepared in comparative example 1; FIG. 4 is a graph of the appearance of the CS stabilized Pickering emulsions of comparative example 1 at 0.125%, 0.25%, and 0.5% after 24 hours of standing; FIG. 6 is a microscopic image of new 0.125%, 0.25% and 0.5% CS-stabilized Pickering emulsions of comparative example 1. As can be seen from fig. 3 and 4, in the comparative examples, when new 0.125%, 0.25% and 0.5% CS-stabilized Pickering emulsions were left for 24 hours, the oil layer was completely separated, a distinct demulsification state was seen, and micrographs of the new emulsions demonstrated no double emulsion structure. Pickering emulsions stabilized with CS of 0.125%, 0.25% and 0.5% are not stable and cannot be prepared as double emulsions. As can be seen from fig. 3 and 4, the Pickering emulsions prepared with different chitosan concentrations in comparative example 1 are unstable and completely demulsified after being left for 24 hours, and it can be clearly seen from fig. 6 that the chitosan solution alone cannot prepare double emulsions. It can be seen that the stable dual Pickering emulsion can not be prepared by one-step method by using CS alone with mass concentrations of 0.125%, 0.25% and 0.5%, and the stable dual Pickering emulsion can be prepared by one-step method by using the composite colloidal particles formed by CS and SWIA.
Adding Nile red, Nile blue and a fluorescent whitening agent into a fresh emulsion sample to respectively dye grease, protein and chitosan, and observing by a laser confocal microscope. The excitation wavelength of Nile blue is 633nm, the excitation wavelength of Nile red is 488nm, and the fluorescence white excitation wavelength is 405 nm. Fig. 7 is a laser confocal plot of a swaa-CS composite colloidal particle stabilized dual Pickering emulsion at different CS mass concentrations. As can be clearly seen from FIG. 7, the O/W emulsion droplets in all the soybean water-insoluble aggregate-chitosan stabilized dual Pickering emulsions contained a large number of W/O small emulsion droplets, indicating that the soybean water-insoluble aggregate-chitosan composite colloidal particles can be homogenized into the W/O/W dual Pickering emulsions by a simple one-step method. In the figure, blue and red are the distribution of soybean water-insoluble aggregates and chitosan in the double emulsion, and the composite particles can be seen to be uniformly adsorbed on an oil interface, so that the formation of the double Pickering emulsion is further confirmed, a firm barrier is formed on the interface, internal phases are independent from each other, and the coalescence phenomenon is inhibited.
And (3) respectively taking 1mL of the stable double Pickering emulsion of the SWIA-CS composite colloidal particles with different CS mass concentrations to perform rheological property test, wherein the temperature is 25 ℃, and the stress scanning frequency is set to be 1Hz, so as to obtain a stress scanning graph. FIGS. 8 to 10 are stress scan graphs of dual Pickering emulsions stabilized by composite colloidal particles of 0.25% SWIA-0.125% CS, 0.25% SWIA-0.25% CS, and 0.25% SWIA-0.5% CS, respectively. As can be seen, the elastic modulus G 'is greater than the viscous modulus G', indicating that the emulsion in this state exhibits gel properties dominated by viscoelasticity.
FIGS. 11 and 12 are periodic microscopic observations of emulsions of 0.25% SWIA-0.25% CS and 0.25% SWIA-0.5% CS, respectively, composite colloidal particle stabilized Pickering double emulsions during intestinal and gastric digestion, respectively. Fats and oils exist in a wide variety of forms in foods, and their digestion, absorption and metabolism play a very important role in directing food formulations. As can be seen in fig. 11 and 12, during gastric digestion, the internal aqueous phase of the dual Pickering emulsion facilitates the transfer of a portion of the internal aqueous phase from the internal phase of low salt concentration to the environment of relatively high salt concentration digestive juices due to the high salt environment of the digestive juices. However, partial internal water phase of emulsion droplets still exists after the gastric digestion is finished, and the size of oil droplets is not substantially changed in the whole gastric digestion process, which preliminarily shows that the dual Pickering emulsion prepared from the composite colloid particles can keep the integrity of an interface structure in the gastric digestion process and is beneficial to conveying fat-soluble active substances to the intestinal digestion.
The soybean water-insoluble aggregate-chitosan composite colloidal particle stable double Pickering emulsion can be used as an active ingredient conveying carrier with hydrophobicity, photosensitivity and intolerance to environmental influence in the fields of food, medicine, cosmetics and other fields, and can be used as a double emulsion template prepared by porous microspheres; the dual Pickering emulsions of the prior art are stabilized by inorganic particles and are prepared in two steps: first form W1Preparation of W after the emulsion1/O/W2Emulsion (CN 110302156A; 20319; Yongchun, Wangqingyun, Baiqingling, Lizhen, Jia legend. double Pickering emulsion template method for preparing inorganic-organic composite hollow microsphere [ J]The chemical journal of higher schools, 2018,39(07): 1462-; the particle emulsifier used in the invention is green and natural food-grade soybean water-insoluble aggregate-chitosan composite colloidal particles, and the preparation method of the invention is a one-step method for preparing W1/O/W2The double Pickering emulsion meets the requirements of the industrial fields of food, medicine and the like on natural, safe and nontoxic raw materials, and has simple and efficient preparation process. The soybean water-insoluble aggregate-chitosan composite colloidal particle stable double Pickering emulsion can be used as an active ingredient conveying carrier with hydrophobicity, photosensitivity and intolerance to environmental influence in the fields of food, medicines and cosmetics, and can be used as a double emulsion template prepared from porous microspheres.
In the prior art, non-food-grade inorganic or polymer particles are combined with a surfactant to serve as an emulsifier of a double Pickering emulsion, and the preparation method is a two-step preparation method and is complicated in preparation process; the invention develops food-grade composite colloidal particles, realizes one-step preparation of the double Pickering emulsion, and provides good technical support for large-scale production.
The embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents and are included in the scope of the present invention.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110636030.1A CN113397156B (en) | 2021-06-08 | 2021-06-08 | Dual Pickering emulsion and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110636030.1A CN113397156B (en) | 2021-06-08 | 2021-06-08 | Dual Pickering emulsion and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113397156A true CN113397156A (en) | 2021-09-17 |
| CN113397156B CN113397156B (en) | 2024-08-02 |
Family
ID=77676915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110636030.1A Active CN113397156B (en) | 2021-06-08 | 2021-06-08 | Dual Pickering emulsion and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113397156B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114424820A (en) * | 2021-12-13 | 2022-05-03 | 东北农业大学 | A kind of preparation method of low-fat perilla oil flavor salad dressing |
| CN115428934A (en) * | 2022-07-21 | 2022-12-06 | 中国农业科学院农产品加工研究所 | A kind of bicontinuous emulsion applied in the fields of food and medicine and its preparation method |
| CN116268368A (en) * | 2023-04-03 | 2023-06-23 | 东北农业大学 | Preparation method of curcumin Pickering emulsion loaded by soybean isolate protein-chitosan |
| CN116349868A (en) * | 2023-03-23 | 2023-06-30 | 宁波索宝蛋白科技股份有限公司 | Emulsion for carrying vitamin C and application thereof |
| CN117084979A (en) * | 2023-07-17 | 2023-11-21 | 江南大学 | Preparation and application of pickering multiple emulsion with stable phytosterol/chitosan composite particles |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1402790A2 (en) * | 2002-09-27 | 2004-03-31 | Nestec S.A. | Interface stabilisation of a product with 2 or more phases with a protein-polysaccharide complex |
| CN101360481A (en) * | 2005-11-22 | 2009-02-04 | 雀巢技术公司 | Oil-in-water emulsion and its use for the delivery of functionality |
| CN110432377A (en) * | 2019-08-13 | 2019-11-12 | 中国海洋大学 | A kind of soybean protein isolate chitosan nano gel and its preparation method and application |
| CN112772923A (en) * | 2021-02-02 | 2021-05-11 | 山东农业大学 | Soybean peptide aggregate-based beta-carotene embedding emulsion and preparation method thereof |
-
2021
- 2021-06-08 CN CN202110636030.1A patent/CN113397156B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1402790A2 (en) * | 2002-09-27 | 2004-03-31 | Nestec S.A. | Interface stabilisation of a product with 2 or more phases with a protein-polysaccharide complex |
| CN101360481A (en) * | 2005-11-22 | 2009-02-04 | 雀巢技术公司 | Oil-in-water emulsion and its use for the delivery of functionality |
| CN110432377A (en) * | 2019-08-13 | 2019-11-12 | 中国海洋大学 | A kind of soybean protein isolate chitosan nano gel and its preparation method and application |
| CN112772923A (en) * | 2021-02-02 | 2021-05-11 | 山东农业大学 | Soybean peptide aggregate-based beta-carotene embedding emulsion and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| CHUN-LI SONG等: "Effect of glycosylation and limited hydrolysis on structural and functional properties of soybean protein isolate.", JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, vol. 12, no. 4, 31 December 2018 (2018-12-31), pages 2946 - 2954, XP036637086, DOI: 10.1007/s11694-018-9910-5 * |
| 舒欣怡等: "一步法制备大豆不溶性肽-壳聚糖复合颗粒稳定的Pickering双重乳液及其表征", 现代食品科技, vol. 37, no. 12, 3 November 2021 (2021-11-03), pages 169 - 176 * |
| 郭兴凤等: "大豆蛋白—壳聚糖共价复合物制备研究", 粮食与油脂, no. 8, 10 August 2010 (2010-08-10), pages 8 - 11 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114424820A (en) * | 2021-12-13 | 2022-05-03 | 东北农业大学 | A kind of preparation method of low-fat perilla oil flavor salad dressing |
| CN115428934A (en) * | 2022-07-21 | 2022-12-06 | 中国农业科学院农产品加工研究所 | A kind of bicontinuous emulsion applied in the fields of food and medicine and its preparation method |
| CN115428934B (en) * | 2022-07-21 | 2023-10-03 | 中国农业科学院农产品加工研究所 | A bicontinuous emulsion used in the food and pharmaceutical fields and its preparation method |
| CN116349868A (en) * | 2023-03-23 | 2023-06-30 | 宁波索宝蛋白科技股份有限公司 | Emulsion for carrying vitamin C and application thereof |
| CN116268368A (en) * | 2023-04-03 | 2023-06-23 | 东北农业大学 | Preparation method of curcumin Pickering emulsion loaded by soybean isolate protein-chitosan |
| CN117084979A (en) * | 2023-07-17 | 2023-11-21 | 江南大学 | Preparation and application of pickering multiple emulsion with stable phytosterol/chitosan composite particles |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113397156B (en) | 2024-08-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113397156A (en) | Dual Pickering emulsion and preparation method thereof | |
| CN110498935B (en) | High internal phase emulsion with quercetin stabilized by soy protein isolate-pectin compound and preparation method thereof | |
| Zhou et al. | High internal phase Pickering emulsion by Spanish mackerel proteins-procyanidins: Application for stabilizing astaxanthin and surimi | |
| Fu et al. | Encapsulation of β-carotene in wheat gluten nanoparticle-xanthan gum-stabilized Pickering emulsions: Enhancement of carotenoid stability and bioaccessibility | |
| Pan et al. | Study on the fabrication and in vitro digestion behavior of curcumin-loaded emulsions stabilized by succinylated whey protein hydrolysates | |
| Dong et al. | Oleogel-based Pickering emulsions stabilized by ovotransferrin–carboxymethyl chitosan nanoparticles for delivery of curcumin | |
| Zhang et al. | High internal phase Pickering emulsions stabilized by a cod protein–chitosan nanocomplex for astaxanthin delivery | |
| Wang et al. | Fabrication of emulsions prepared by rice bran protein hydrolysate and ferulic acid covalent conjugate: Focus on ultrasonic emulsification | |
| Huang et al. | Effect of high-intensity ultrasound on the physicochemical properties of Tenebrio Molitor Protein | |
| CN106579327A (en) | High internal phase gel-like zein Pickering emulsion and preparation method thereof | |
| Xu et al. | Pickering emulsions synergistic stabilized with konjac glucomannan and xanthan gum/lysozyme nanoparticles: Structure, protection and gastrointestinal digestion | |
| CN111205479B (en) | High internal phase Pickering emulsion with stable modified bacterial cellulose nanofiber and preparation method thereof | |
| Zhang et al. | Enzymatically modified quinoa starch based pickering emulsion as carrier for curcumin: Rheological properties, protection effect and in vitro digestion study | |
| Chen et al. | Development of anti-photo and anti-thermal high internal phase emulsions stabilized by biomass lignin as a nutraceutical delivery system | |
| CN109588721B (en) | A kind of carotenoid-protein particle and its preparation method and application | |
| Zhang et al. | Effect of ultrasound and alkali-heat treatment on the thermal gel properties and catechin encapsulation capacity of ovalbumin | |
| CN102488186B (en) | Edible nano-scale beta-carotene emulsion free from high pressure homogenization and preparation method thereof | |
| CN114698843B (en) | Perilla seed oil microemulsion-hydrogel system and preparation method and application thereof | |
| Zhou et al. | A novel emulsion gel solely stabilized by the hot water extracted polysaccharide from psyllium husk: Self-healing plays a key role | |
| Yang et al. | Potential low-calorie model that inhibits free fatty acid release and helps curcumin deliver in vitro: Ca2+-induced emulsion gels from low methyl-esterified pectin with the presence of erythritol | |
| Wang et al. | Preparation, characterization and in vitro digestive behaviors of emulsions synergistically stabilized by γ-cyclodextrin/sodium caseinate/alginate | |
| Xu et al. | Heat stability promoted Pickering emulsions stabilized by glidian/sodium caseinate nanoparticles and konjac glucomannan | |
| CN113501973B (en) | Pickering emulsion stabilized by nano-cellulose hydrogel | |
| Tian et al. | The impact of ultrasound treatment combined with flaxseed gum on the foaming properties of egg white | |
| Gao et al. | Effects of guar gum or xanthan gum addition in conjunction with pasteurization on liquid egg white |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |