CN113384482B

CN113384482B - Moisturizing and skin-tendering essence and preparation method thereof

Info

Publication number: CN113384482B
Application number: CN202110743276.9A
Authority: CN
Inventors: 王海清; 丁伯娇; 田旭萍; 王思逸; 牛蔚青; 陈学通; 郭子湖; 黄超; 王永法; 王永华
Original assignee: Shaanxi Qinling Qiyao Collaborative Innovation Center Co ltd
Current assignee: Shaanxi Qinling Qiyao Collaborative Innovation Center Co ltd
Priority date: 2021-06-30
Filing date: 2021-06-30
Publication date: 2023-08-22
Anticipated expiration: 2041-06-30
Also published as: CN113384482A

Abstract

The invention provides a moisturizing and skin-tendering essence which comprises the following components in parts by weight: 1-100 parts of recombinant silk fibroin, 1-100 parts of white peony root extract, 1-100 parts of bletilla tuber extract, 1-100 parts of purslane extract, 1-100 parts of honeysuckle extract, 1-100 parts of poncirus trifoliata extract, 1-100 parts of red clover extract, 1-100 parts of papaya extract, 1-100 parts of royal jelly extract and 1-100 parts of wheat germ extract; it has excellent effects of keeping moisture, moistening, caring skin, removing wrinkle, whitening skin, and resisting allergy, and can prevent external irritation to skin, and consolidate curative effect; the invention also provides a preparation method of the moisturizing and skin-tendering essence, which can exert the efficacy to the greatest extent and improve the curative effect of moisturizing and skin-tendering and resisting aging.

Description

Moisturizing and skin-tendering essence and preparation method thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to a moisturizing and skin tendering essence and a preparation method thereof.

Background

The skin plays an important barrier function in the human body, protects the human body from external factors such as ultraviolet rays and dry environments, and prevents excessive moisture from escaping from the body through the skin. The protective function can be maintained while the keratinocyte-containing stratum corneum remains in a normal state. The barrier function of skin mainly comprises permeation function to physical, chemical, biological, moisture and the like, the water content of normal skin is 20% -35%, and when the water content of skin is reduced to below 10%, the barrier function is damaged. The decrease in skin barrier function can lead to the development of many skin disorders such as skin pruritus, atopic dermatitis, skin dullness, long spots, psoriasis, acne and dry skin.

The stratum corneum (the stratum corneum) that is the outermost layer of the epidermis is formed from keratinocytes and contains differentiated keratinocytes and a lipid layer surrounding these keratinocytes. Keratinocytes are typical cells resulting from such a process: in this process, basal cells that proliferate continuously in the basal layer of the epidermis are pushed up to the skin surface, and the shape and function thereof are gradually changed. After a certain period of time, old keratinocytes are shed from the skin surface, and new keratinocytes act instead of shed cells. This repeated and continuous process is known as differentiation or keratinization of the epidermal cells. In addition, keratinocytes form the stratum corneum while producing Natural Moisturizing Factors (NMFs) and intracellular lipids (neurothalamine, cholesterol, fatty acids, etc.) during keratinization, which gives the stratum corneum firmness and flexibility, ultimately making it a skin barrier. The stratum corneum is very prone to loss of function due to various factors, for example living and habitual factors such as excessively frequent washing and bathing, environmental factors such as dry weather, pollutants, etc., and endogenous diseases such as atopic dermatitis and senile skin itching. With the current tremendous increase in the number of deleterious factors, numerous conditions and diseases associated with dry skin are also increasing.

Many researchers have conducted many studies focused on moisturizing from external sources or preventing water loss from the human body to maintain the moisture content of the skin at an appropriate level, and various moisturizers having water holding ability have been developed. Lipid components (e.g., neurophthalamide) and substances that enhance water retention in the stratum corneum (e.g., essential fatty acids and lipid complexes) are commonly used as humectants. However, the factors harmful to the skin are increasing, the rate of production and shedding of the horny layer is slowing down due to the change of eating habits, the amount of moisturizing factors and lipids is also decreasing due to the deterioration of keratinocyte function, and moisturizing and skin rejuvenating products are increasingly incapable of exerting normal barrier function on the skin horny layer of people for the above reasons. This degradation of the skin barrier function can cause a variety of skin disorders including xerosis cutis, atopic dermatitis, contact dermatitis, and psoriasis. Although moisturizers having water holding capacity have conventionally been able to alleviate the above-mentioned diseases, it is still difficult to fundamentally cure the diseases.

Although skin aging is an irreversible physiological phenomenon, its progression can be delayed or slowed down. The method of combining active substances in natural plants with mild metabolism improving materials is a development trend of modern skin care technologies. The research and development personnel utilize modern advanced technology to extract essence components in plants, so that active ingredients are fully contacted with skin, and efficacy is better exerted. For busy and beautiful women, the skin-refreshing, moisturizing and skin-tendering essence lotion containing plant essence is scientific and feasible. The essence contains more nutrients and active ingredients than the toner and the cream, has strong efficacy and obvious moisturizing and skin tendering effects. However, the existing essence has the problems of poor permeability, difficult absorption and the like, and the instant moisturizing effect and the durability of the moisturizing effect are required to be improved.

Disclosure of Invention

The invention aims to provide a moisturizing and skin-tendering essence and a preparation method thereof, which have excellent moisturizing, skin-tendering, anti-wrinkle, whitening and antiallergic effects, can prevent external stimulation to skin, and consolidate curative effects; the invention also provides a preparation method of the moisturizing and skin-tendering essence, which can exert the efficacy to the greatest extent and improve the curative effect of moisturizing and skin-tendering and resisting aging.

The embodiment of the invention is realized by the following technical scheme:

the moisturizing and skin-tendering essence lotion comprises the following components in parts by weight: 1-100 parts of recombinant silk fibroin, 1-100 parts of white peony root extract, 1-100 parts of bletilla tuber extract, 1-100 parts of purslane extract, 1-100 parts of honeysuckle extract, 1-100 parts of poncirus trifoliata extract, 1-100 parts of red clover extract, 1-100 parts of papaya extract, 1-100 parts of royal jelly extract and 1-100 parts of wheat germ extract.

A preparation method of moisturizing and skin-tendering essence lotion comprises the following steps:

s1: optimizing a fibroin gene sequence by using a pichia pastoris conventional codon on the gene level, synthesizing the gene artificially, integrating the gene into a yeast chromosome, constructing a recombinant pichia pastoris engineering bacterium with constitutive secretory expression, transferring the recombinant pichia pastoris engineering bacterium into a fermentation medium, actively secreting and expressing the recombinant fibroin into a fermentation broth along with the vital activity of the engineering bacterium, centrifuging to collect a supernatant, adsorbing the recombinant fibroin by using resin under a non-deformation condition, washing impurities by using a washing solution, eluting the recombinant fibroin, collecting an eluent, desalting and concentrating, and finally performing vacuum freeze drying to obtain the recombinant fibroin for later use;

s2: pulverizing radix Paeoniae alba, rhizoma Bletillae, herba Portulacae, flos Lonicerae, fructus Ponciri Trifoliatae, herba Trifoliatae, fructus Muscovitum, lac Regis Apis, and wheat embryo respectively, sieving, adding 10-20 times of solvent (75-85% ethanol) by weight, soaking at 35-45deg.C for 7-9 hr, performing low temperature ultrasonic extraction for 5-7 hr, centrifuging, filtering to obtain supernatant, filtering again, and lyophilizing to obtain corresponding plant extract, mixing;

s3: placing water, a softening agent, a film forming agent and a solvent in a clean container, heating to 45-55 ℃, stirring and dissolving for later use;

s4: adding the plant extract prepared in the step S2 into a container of the step S3, adding a surfactant, heating to 60-65 ℃ at the rotating speed of 60-90rpm, preserving heat and stirring for 15-25 min, and homogenizing for 2-5 min until the material is uniformly dispersed;

s5: adding a humectant into the S4, homogenizing for 2-5 minutes under the stirring condition, starting a vacuum device to enable the vacuum degree of the container to be 0.04 Pa-0.1 MPa, and keeping the vacuum state to keep the temperature and stirring for 10 minutes;

s6: cooling to 35-40deg.C, adding thickener and recombinant fibroin into the mixture prepared in step S5, and stirring;

s7: adding a skin conditioner into the mixture prepared in the step S6, and uniformly stirring;

s8: cooling to 18-25deg.C, adding antiseptic, pH regulator, and chelating agent, and stirring to obtain the final product.

The technical scheme of the embodiment of the invention has at least the following advantages and beneficial effects:

1. the moisturizing and skin-tendering essence disclosed by the invention is safe and reliable, has good stability, has good moisturizing, skin tendering and water locking effects, can give skin moisture and grease, has the effects of immediately and long-acting moisturizing and tendering, improving skin darkness and recovering skin elasticity, can effectively promote the removal of aged skin cells, accelerate the metabolism of skin and organisms, and can play the roles of cleaning skin, tendering muscles, whitening, resisting allergy, eliminating color spots, resisting wrinkles, inhibiting acne and the like.

2. The moisturizing and skin-tendering essence disclosed by the invention can coordinate and promote the generation of skin collagen and the division and proliferation of cells of each skin layer, and has a good repairing effect on aged and damaged skin; can increase the intracellular moisture of the skin layer, lighten the fine lines of the skin, promote the division and proliferation of epidermal cells, reduce the length and depth of skin wrinkles, restore the skin elasticity, and obviously lighten and reduce the wrinkles of the skin after long-term use.

3. The essence disclosed by the invention can be used for filling the concave parts of the skin wrinkles, can achieve the effect of timely filling lines, can effectively scatter light, and has the effect of matte soft focus, so that the visual effect of short-term instant effect wrinkle reduction is achieved.

4. The plant natural component composition in the essence can stimulate lipid production, adiponectin production and adipocyte differentiation, and has excellent antioxidant effect and skin moisturizing effect.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

The moisturizing and skin-tendering essence and the preparation method thereof provided by the embodiment of the invention are specifically described below.

Further, the method comprises the following steps of: 40 parts of recombinant silk fibroin, 12 parts of white peony root extract, 15 parts of bletilla striata extract, 10 parts of purslane extract, 11 parts of honeysuckle flower extract, 15 parts of poncir trifoliata extract, 8 parts of red clover extract, 10 parts of papaya fruit extract, 15 parts of royal jelly extract and 20 parts of wheat germ extract.

Further, the method comprises the following steps of: 30 parts of recombinant silk fibroin, 20 parts of white peony root extract, 16 parts of bletilla striata extract, 15 parts of purslane extract, 10 parts of honeysuckle extract, 10 parts of poncir trifoliata extract, 8 parts of red clover extract, 15 parts of papaya extract, 20 parts of royal jelly extract and 15 parts of wheat germ extract.

Further, the method comprises the following steps of: 35 parts of recombinant silk fibroin, 15 parts of white peony root extract, 15 parts of bletilla striata extract, 16 parts of purslane extract, 16 parts of honeysuckle extract, 20 parts of poncir trifoliata extract, 5 parts of red clover extract, 15 parts of papaya extract, 10 parts of royal jelly extract and 10 parts of wheat germ extract.

Further, the method for obtaining the recombinant fibroin comprises the following steps: the recombinant pichia pastoris engineering bacteria with constitutive secretory expression are constructed by optimizing the gene sequence of the fibroin by using the conventional codon of pichia pastoris on the gene level and synthesizing the gene artificially, the recombinant pichia pastoris engineering bacteria are transferred into a fermentation medium, the recombinant fibroin is actively secreted and expressed in a fermentation broth along with the vital activity of the engineering bacteria, the supernatant is collected by centrifugation, the recombinant fibroin is adsorbed by resin under the non-deformation condition, the impurities are washed by using a washing solution, the recombinant fibroin is eluted, the eluent is collected, desalted and concentrated, and finally the recombinant fibroin is obtained by vacuum freeze drying.

According to the description of the handbook of cosmetic plant raw materials and research literature, the functions and the effects of each component in the raw material composition are as follows:

recombinant silk fibroin: can be used for moisturizing and nourishing skin care products, and also can be used for caring hair. The product has high absorption of water and sebum, soft hand feeling, transparent and uniform film, and good anti-inflammatory effect.

White peony root extract: has the functions of strengthening physique, enhancing immunity, resisting inflammation, relieving cough, eliminating phlegm, relieving asthma, and the like, can treat acne, has anti-inflammatory and antiallergic effects, and can delay the aging of organism tissues.

Rhizoma bletillae extract: has inhibiting effect on 5α -reductase, can be used for treating acne caused by high androgen, and is also antioxidant, skin whitening agent and antiinflammatory and antiallergic agent with obvious effect.

Purslane extract: the content mainly comprises alkaloid, coumarin compounds, flavonoid, phenols, mushrooms, sterols and other compounds. Has broad-spectrum antibacterial and antiinflammatory effects, and can be used for preventing and treating dermatoses such as skin eczema, allergic dermatitis, contact dermatitis, etc.; and simultaneously has good oxygen free radical scavenging ability and skin moisture retention.

Honeysuckle extract: the product is rich in caffeic tannic acid, and has antibacterial, fat reducing, skin caring and skin aging preventing effects; meanwhile, the luteolin has the effects of resisting bacteria and inflammation, has strong penetrability to skin, can eliminate free radicals and has the function of ultraviolet absorption, so that the luteolin can inhibit and eliminate skin color spots, especially senile plaques, and has a certain whitening effect.

Extract of poncirus trifoliata: is rich in calcium, potassium, iron, zinc, selenium, vitamin B and vitamin C, and has strong absorption capacity of oxidative free radicals; can also help prevent oxidation of DNA and recover damaged DNA, thereby having anti-aging effect.

Red clover extract: contains flavonoid, protein, amino acid, saccharide, vitamins, etc., and has effects of sterilizing, resisting oxidation, protecting free radical injury, scavenging free radical, whitening skin, resisting aging, and astringing pores.

Papaya fruit extract: the papain-rich skin care cream is rich in papain, can hydrolyze keratin and promote softening and dissolving of keratinized skin, thereby playing a role in removing cells of aged skin, accelerating metabolism of skin and organisms, and has the effects of cleaning skin, tendering muscles, eliminating color spots, resisting wrinkles, inhibiting acne and the like.

Royal jelly extract: is rich in superoxide dismutase, transaminase, cholinesterase, antibacterial peptide and various royal jelly main proteins, has the functions of resisting aging, resisting tumor, resisting bacteria, regulating immunity and promoting cell proliferation, can endow cell energy, recover cell activity and help skin growth, thereby playing the roles of conditioning agent, humectant and antioxidant in skin.

Wheat germ extract: has good effect on proliferation of fiber bud cells, and has good skin-activating and anti-aging effects. Simultaneously can inhibit collagenase and remove 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH), and has effects of caring and smoothing skin, and inhibiting and delaying wrinkle.

The moisturizing and skin-tendering essence disclosed by the invention adopts ten moisturizing, whitening and skin-tendering water-locking raw materials such as recombinant fibroin, white paeony root extract, rhizoma bletillae extract, purslane extract, honeysuckle extract, poncirus trifoliata extract, red clover extract, papaya fruit extract, royal jelly extract and wheat germ extract on the water supplementing, moisturizing and skin-tendering effects, wherein the purslane extract and the royal jelly extract can directly bring water molecules adsorbed by the purslane extract and the royal jelly extract into epidermis, dermis and subcutaneous tissues of skin, so that the moisture can be quickly supplemented to the skin, and a good moisturizing effect can be brought to the skin;

white peony root extract, bletilla tuber extract, red clover extract and poncirus trifoliata extract can quickly permeate to the muscle bottom to generate the functions of antioxidation, anti-inflammatory and blood circulation promotion, so that the darkness and dullness of skin are improved, uneven skin color is repaired, the skin color is lightened, the skin elasticity is increased, and finally the skin is fair and glossy, and the texture is warm and fine;

the honeysuckle extract, the papaya fruit extract and the wheat germ extract have strong penetrability to skin, can eliminate free radicals and have the function of ultraviolet absorption, so that the skin color spots can be inhibited and eliminated, and simultaneously, the inhibition effect of collagenase, the elimination effect of DPPH and the hydrolysis effect of keratin can effectively promote the removal of aging skin cells, accelerate the metabolism of skin and organisms, and have the effects of cleaning skin, tenderizing muscles, eliminating color spots, resisting wrinkles, inhibiting acne and the like;

most importantly, the recombinant fibroin forms a transparent and uniform film in the skin, can prevent moisture in the skin from volatilizing and losing from the surface layer of the skin, can form a silky skin feel on the skin, can protect the skin from being stimulated by external environment, and has good moisturizing effect for 24 hours.

Therefore, the moisturizing and skin-tendering essence disclosed by the invention plays roles in moisturizing, moisturizing and water locking, so that excellent moisturizing, skin-tendering, anti-wrinkle, whitening and antiallergic effects are achieved, external irritation to skin can be prevented, and the moisturizing and skin-tendering essence disclosed by the invention is consolidated to exert curative effects.

Further, the method comprises the following steps of: 1-1000 parts of solvent, 1-100 parts of thickener, 1-100 parts of humectant, 1-100 parts of skin conditioning agent, 1-100 parts of surfactant, 1-100 parts of chelating agent, 1-100 parts of softener, 1-100 parts of film forming agent, 1-100 parts of preservative and 1-100 parts of pH regulator.

The preparation method of the moisturizing and skin-tendering essence lotion comprises the following steps of:

s4: adding the recombinant fibroin prepared in the step S1 and the plant extract prepared in the step S2 into a container of the step S3, simultaneously adding methyl glucitol polyether-20 and PEG-40 hydrogenated castor oil, heating to 60-65 ℃ at the rotating speed of 60-90rpm, preserving heat and stirring for 15-25 min, homogenizing for 2-5 min until the materials are uniformly dispersed;

s5: adding saccharide isomerides, hydrolyzed sodium hyaluronate, trehalose and sodium polyglutamate raw materials into S4, homogenizing for 2-5 minutes under the stirring condition, starting a vacuum device to enable the vacuum degree of a container to be 0.04 Pa-0.1 MPa, and keeping the vacuum state to keep the temperature and stirring for 10 minutes;

s6: cooling to 35-40 ℃, adding acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and the recombinant silk fibroin raw material prepared in the step S1 into the mixture prepared in the step S5, and uniformly stirring;

s7: adding lysine and magnesium ascorbyl phosphate into the mixture prepared in the step S6, and uniformly stirring;

s8: cooling to 18-25deg.C, adding antiseptic octaethylene glycol and phenoxyethanol, and stirring; adding pH regulator arginine and sodium citrate, and stirring uniformly; and adding chelating agent EDTA disodium, and stirring uniformly to obtain the moisturizing and skin-tendering essence.

Preferably, the natural plant components are soaked for 8 hours at 40 ℃ and subjected to low-temperature ultrasonic extraction for 6 hours.

Further, the stirring speed in S3, S4 and S5 is 60-90rpm; the stirring speed in S6, S7 and S8 is 20-40rpm, and the homogenizing speed in S4 and S5 is 2000-3000rpm.

Further, the solvent comprises at least one of purified water, butanediol, dipropylene glycol, propylene glycol, glycerol, and ethanol. Preferably, the solvent is ethanol at a concentration of 80% by volume.

Further, the thickener includes, but is not limited to carbomers, acrylic acid (esters) based/C10-30 alkanol acrylate cross-linked polymers, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, xanthan gum, hydroxyethyl cellulose, and the like.

Further, the skin conditioning agent comprises at least one of lysine, ascorbic acid, vitamin C, ceramide, magnesium ascorbyl phosphate, ascorbyl glucoside, ascorbyl palmitate, glutathione, dipotassium glycyrrhizinate, glabridin, and lecithin.

Further, the humectant comprises at least one of saccharide isomer, sodium hyaluronate, hydrolyzed sodium hyaluronate, 1, 2-hexanediol, sodium polyglutamate, squalane, chitosan, trehalose, urea, allantoin, polyether glycerol-26, sodium lactate, sodium pyrrolidone carboxylate, xylitol, and biological gum-1.

Further, the surfactant includes, but is not limited to, methyl glucitol polyether-20, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, di-C12-15 alkanol polyether-8 phosphate, hydrogenated lecithin, polyglycerol-6 polyricinoleate, polyglycerol-10 laurate, and the like.

Further, the chelating agent includes, but is not limited to, disodium EDTA, octanoyl hydroxamic acid, and the like.

Further, the emollient includes, but is not limited to, polyethylene glycol-8, pantethine, and the like.

Further, the film forming agent includes, but is not limited to, polydimethylsiloxane, cyclopentadimethicone, polyquaternium-51, and the like.

Further, the preservative includes, but is not limited to, sodium dehydroacetate, bis (hydroxymethyl) imidazolidinyl urea, phenoxyethanol, octylglycol, potassium sorbate, and the like.

Further, the pH adjustor includes, but is not limited to, tromethamine, sodium citrate, arginine, and the like.

Example 1

The moisturizing and skin-tendering essence lotion comprises the following components in parts by weight: 40 parts of recombinant silk fibroin, 12 parts of white peony root extract, 15 parts of bletilla striata extract, 10 parts of purslane extract, 11 parts of honeysuckle flower extract, 15 parts of poncirus trifoliata extract, 8 parts of red clover extract, 10 parts of papaya fruit extract, 15 parts of royal jelly extract, 20 parts of wheat germ extract, 400 parts of solvent, 3 parts of thickener, 30 parts of humectant, 20 parts of skin conditioner, 6 parts of surfactant, 10 parts of chelating agent, 3 parts of softener, 3 parts of film forming agent, 3 parts of preservative and 3 parts of pH regulator.

Wherein the solvent consists of 360 parts by weight of purified water and 40 parts by weight of glycerin; the thickener is 3 parts by weight of acrylic acid (ester) and C10-30 alkanol acrylate cross-linked polymer; the humectant consists of 10 parts by weight of saccharide isomer, 6 parts by weight of hydrolyzed sodium hyaluronate, 7 parts by weight of trehalose and 7 parts by weight of sodium polyglutamate; the skin conditioner consists of 10 parts by weight of lysine and 10 parts by weight of magnesium ascorbyl phosphate; the surfactant consists of 3 parts by weight of methyl glucitol polyether-20 and 3 parts by weight of PEG-40 hydrogenated castor oil; the chelating agent is 10 parts by weight of disodium EDTA; the softening agent is polyethylene glycol-8 with the weight of 3 parts; 3 parts by weight of a film forming agent, namely polyquaternium-51; the preservative consists of 1 part by weight of octyl glycol and 2 parts by weight of phenoxyethanol; the pH regulator consists of 2 parts by weight of arginine and 1 part by weight of sodium citrate.

The preparation method of the moisturizing and skin-tendering essence lotion comprises the following steps:

s1: the recombinant pichia pastoris engineering bacteria with constitutive secretory expression are constructed by optimizing the gene sequence of the fibroin by using the conventional codon of pichia pastoris on the gene level and synthesizing the gene artificially and integrating the gene into yeast chromosome, and after the recombinant pichia pastoris engineering bacteria are transferred into a fermentation medium, the recombinant fibroin is actively secreted and expressed in a fermentation broth along with the vital activity of the engineering bacteria, the supernatant is collected by centrifugation, the recombinant fibroin is adsorbed by resin under the non-deformation condition, the impurities are washed by using a washing liquid, the recombinant fibroin is eluted, the eluent is collected, desalted and concentrated, and finally the recombinant fibroin is obtained by vacuum freeze drying for standby.

S2: pulverizing radix Paeoniae alba, rhizoma Bletillae, herba Portulacae, flos Lonicerae, fructus Ponciri Trifoliatae, herba Trifoliatae, fructus Muscovitum, lac Regis Apis, and wheat embryo respectively, sieving, adding 15 times of 80% ethanol by weight, soaking at 40deg.C for 8 hr, performing low-temperature ultrasonic extraction for 6 hr, centrifuging supernatant, filtering, refluxing ethanol, filtering again, and lyophilizing to obtain corresponding plant extract mixture;

s3: placing water, polyethylene glycol-8, polyquaternium-51 and glycerin in a clean container, heating to 50 ℃ by using external heating equipment, and stirring and dissolving for later use;

s4: adding the natural component mixture prepared in the step S2, methyl glucitol polyether-20 and PEG-40 hydrogenated castor oil into a container of S3, stirring and heating to 62 ℃ at a rotating speed of 70rpm, preserving heat and stirring for 20min, and homogenizing for 3 min until the materials are uniformly dispersed;

s5: uniformly stirring saccharide isomer, hydrolyzed sodium hyaluronate, trehalose and sodium polyglutamate raw materials, adding into the container in the step S4, homogenizing for 3 minutes under the stirring condition, starting a vacuum device to ensure that the vacuum degree of the container is 0.06MPa, keeping the vacuum state, and continuously preserving heat and stirring for 10 minutes;

s6: when the temperature is reduced to 38 ℃, adding acrylic acid (ester)/C10-30 alkanol acrylate crosslinked polymer and recombinant silk fibroin raw material into the mixture prepared in the step S5, and uniformly stirring;

s7: adding lysine and magnesium ascorbyl phosphate raw materials into the mixture prepared in the step S6, and uniformly stirring;

s8: cooling to 22deg.C, adding antiseptic octaethylene glycol and phenoxyethanol, and stirring; adding pH regulator arginine and sodium citrate, and stirring uniformly; and adding chelating agent EDTA disodium, and stirring uniformly to obtain the moisturizing and skin-tendering essence.

Example 2

This embodiment differs from embodiment 1 in that: the plant components in the moisturizing and skin-tendering essence lotion comprise the following components in parts by weight: 30 parts of recombinant silk fibroin, 20 parts of white peony root extract, 16 parts of bletilla striata extract, 15 parts of purslane extract, 10 parts of honeysuckle extract, 10 parts of poncir trifoliata extract, 8 parts of red clover extract, 15 parts of papaya extract, 20 parts of royal jelly extract and 15 parts of wheat germ extract.

Example 3

This embodiment differs from embodiment 1 in that: the plant components in the moisturizing and skin-tendering essence lotion comprise the following components in parts by weight: 35 parts of recombinant silk fibroin, 15 parts of white peony root extract, 15 parts of bletilla striata extract, 16 parts of purslane extract, 16 parts of honeysuckle extract, 20 parts of poncir trifoliata extract, 5 parts of red clover extract, 15 parts of papaya extract, 10 parts of royal jelly extract and 10 parts of wheat germ extract.

Example 4

This embodiment differs from embodiment 1 in that: the plant components in the moisturizing and skin-tendering essence lotion comprise the following components in parts by weight: 1 part of recombinant silk fibroin, 100 parts of white peony root extract, 100 parts of bletilla striata extract, 1 part of purslane extract, 1 part of honeysuckle extract, 100 parts of poncir trifoliata extract, 1 part of red clover extract, 100 parts of papaya extract, 1 part of royal jelly extract and 100 parts of wheat germ extract.

Example 5

This embodiment differs from embodiment 1 in that: the plant components in the moisturizing and skin-tendering essence lotion comprise the following components in parts by weight: 100 parts of recombinant silk fibroin, 1 part of white peony root extract, 1 part of bletilla striata extract, 100 parts of purslane extract, 100 parts of honeysuckle extract, 1 part of poncir trifoliata extract, 100 parts of red clover extract, 1 part of papaya extract, 100 parts of royal jelly extract and 1 part of wheat germ extract.

Experimental example 1

According to cosmetic safety technical Specification (2015 edition), the moisturizing and skin-tendering essence lotions prepared in examples 1-3 were subjected to microorganism and heavy metal detection. The test results are shown in Table 1:

table 1: the results of the detection of the moisturizing and skin-rejuvenating essential lotions prepared in examples 1 to 3

As can be seen from the data in Table 1, the moisturizing and skin-tendering essence prepared in the examples 1-3 of the invention do not detect microorganisms, and the heavy metal content accords with the standard, which indicates that the moisturizing and skin-tendering essence prepared in the invention is safe and reliable.

Experimental example 2

The physical and chemical indexes of the moisturizing and skin-tendering essence prepared in the embodiments 1-3 of the invention are tested, and the results are as follows:

2.1 traits: the product is milky semitransparent essence with proper viscosity, fineness, uniformity and good spreadability.

2.2pH check: a small amount of essence is dipped by wet PH test paper, and the PH is 6-7.

2.3 cold and hot test: the moisturizing and skin-tendering essence prepared in examples 1-3 is packaged in a transparent cosmetic bottle, and no layering phenomenon is observed when the cosmetic bottle is refrigerated for about one week in a refrigerator at 4 ℃. The mixture is placed in a constant temperature box at 55 ℃ for 24 hours, and the phenomena of layering, smell change and the like are avoided.

2.3 centrifugation test: the moisturizing and skin-tendering essence prepared in the examples 1-3 is packaged in a test tube, and centrifuged for 20min at 3000r/min without layering phenomenon.

2.4 irritation test, allergy test: 1) Animal experiment: the back of the mice is cut off, the moisturizing and skin-tendering essence prepared in the examples 1-3 is respectively smeared on the parts with the hair cut off, and compared with the parts without the hair cut off, the moisturizing and skin-tendering essence has no irritation and anaphylactic reaction; 2) Human body experiment: the moisturizing and skin-tendering essence prepared in examples 1-3 is smeared on the hand of volunteers (18-66 years old, 200 people) and observed after 30 minutes, the phenomena of redness, eruption and blistering do not occur.

2.5 room temperature standing experiments: the moisturizing and skin-tendering essence prepared in examples 1-3 was placed in a cosmetic bottle, and left standing at room temperature for 6 months without layering, without change in feeling after use, and without change in smell.

Experimental example 3

3.1 Experimental purposes: the moisturizing and skin-tendering essence lotions prepared in examples 1-3 were subjected to long-term moisturizing effect tests, and the moisture content of human skin under the same environment was tested.

3.2 subjects: 120 women aged 18-40 were selected and divided into four groups: blank (purified water), examples 1-3 (moisturizing skin-rejuvenating serum prepared in examples 1-3). No cosmetic or topical medicine can be used 2-3 days before the test.

3.3 experimental method:

before the test: the face is cleaned, the subject sits still for 30min under different dehumidifying environments before formal test, the skin moisture content value of the blank area before use is tested by using a CK skin tester, and the tested part is the forehead rich in sebaceous glands.

Test treatment: treatment with purified water was performed on the face of subjects in the placebo group; the faces of subjects in the group of examples 1 to 3 were treated with the moisturizing and skin-rejuvenating lotions prepared in examples 1 to 3 of the present invention, respectively; and the moisture content of the skin surface is measured at the measured part by using a CK skin tester at different time intervals (6 time, 12 time and 24 time), the working environment temperature is 22-24 ℃, the relative humidity is 33-46%, and the unit of the skin moisture content is au (arbitrary unit). The test results are shown in Table 2:

table 2: skin surface moisture content variation

Name of the name	Before use	After use for 0h	6h	12h	24h
						Blank control group	41.6±3.8	73.8±2.6	38.9±1.5	40.8±3.4	37.5±4.1
Example 1	38.5±1.3	86.4±3.1	79.6±2.6	67.7±1.7	51.5±1.3
						Example 2	40.8±1.8	85.2±2.7	75.6±1.9	65.9±2.8	53.5±1.6
Example 3	38.9±1.9	85.7±4.1	73.2±3.2	63.4±2.6	50.5±1.9

As can be seen from the data in Table 2, the moisturizing and skin-tendering essence prepared in examples 1-3 of the present invention has a greatly improved moisturizing effect compared with the blank control group, which indicates that the moisturizing and skin-tendering essence prepared in the present invention has a long-acting moisturizing effect.

Experimental example 4

4.1 experimental purposes: comprehensive effects of long-term use are studied aiming at the moisturizing and skin-tendering essence prepared in the examples 1-3.

4.2 subjects: selecting 150 women in 18-66 years old as trial objects by using a poll scoring method, and dividing the women into three groups: example 1, example 2 and example 3 (moisturizing skin-rejuvenating lotions prepared in examples 1-3, respectively).

4.3 experimental method:

the skin care essence is applied to the face of a subject, and is used twice a day in the morning and evening for two months. Finally, investigation and research on moisture retention, skin tendering, fineness, wrinkle resistance, whitening and antiallergic property are carried out. Wherein, each use effect is totally divided into 5 points: a score of 5 being the highest score indicates that it is very satisfactory; 4 is satisfied; a score of 3 being acceptable; a score of 3 or less is not good and unacceptable. The test results are shown in Table 3:

table 3: comprehensive effect investigation scoring table of moisturizing and skin-tendering essence prepared in examples 1-3

Effect of use	Example 1	Example 2	Example 3
				Moisture retention	4.62±0.64	4.44±0.61	4.50±0.61
Moistening property	4.48±0.61	4.40±0.61	4.40±0.57
				Skin tendering property	4.38±0.67	4.60±0.70	4.32±0.62
Fineness of	4.56±0.54	4.22±0.71	4.46±0.65
				Wrinkle resistance	4.48±0.65	4.66±0.69	4.34±0.63
Whitening property	4.60±0.53	4.50±0.54	4.46±0.61
				Antiallergic effect	4.32±0.59	4.50±0.61	4.50±0.58

As can be seen from the data in Table 3, the moisturizing and skin-tendering essence lotions prepared in examples 1-3 of the present invention have good effects on moisturizing, skin tendering, fineness, wrinkle resistance, whitening and antiallergic properties. The skin care essence lotion used by the tested volunteers is smooth and comfortable and has good touch feeling. No special reaction is caused on the skin no matter what the skin is used, the moisturizing and skin tendering effects are good, and the skin is obviously glossy and ruddy after the skin is used.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The moisturizing and skin-tendering essence is characterized by comprising the following components in parts by weight: 1-100 parts of recombinant silk fibroin, 1-100 parts of white peony root extract, 1-100 parts of bletilla tuber extract, 1-100 parts of purslane extract, 1-100 parts of honeysuckle extract, 1-100 parts of poncirus trifoliata extract, 1-100 parts of red clover extract, 1-100 parts of papaya extract, 1-100 parts of royal jelly extract and 1-100 parts of wheat germ extract;

the method for obtaining the recombinant fibroin comprises the following steps: the recombinant pichia pastoris engineering bacteria with constitutive secretory expression are constructed by optimizing the gene sequence of the fibroin by using the conventional codon of pichia pastoris on the gene level and synthesizing the gene artificially, the recombinant pichia pastoris engineering bacteria are transferred into a fermentation medium, the recombinant fibroin is actively secreted and expressed in a fermentation broth along with the vital activity of the engineering bacteria, the supernatant is collected by centrifugation, the recombinant fibroin is adsorbed by resin under the non-deformation condition, the impurities are washed by using a washing solution, the recombinant fibroin is eluted, the eluent is collected, desalted and concentrated, and finally the recombinant fibroin is obtained by vacuum freeze drying.

2. The moisturizing and skin-rejuvenating essence according to claim 1, which is characterized by comprising, in parts by weight: 40 parts of recombinant silk fibroin, 12 parts of white peony root extract, 15 parts of bletilla striata extract, 10 parts of purslane extract, 11 parts of honeysuckle flower extract, 15 parts of poncir trifoliata extract, 8 parts of red clover extract, 10 parts of papaya fruit extract, 15 parts of royal jelly extract and 20 parts of wheat germ extract.

3. The moisturizing and skin-rejuvenating essence according to claim 1, which is characterized by comprising, in parts by weight: 30 parts of recombinant silk fibroin, 20 parts of white peony root extract, 16 parts of bletilla striata extract, 15 parts of purslane extract, 10 parts of honeysuckle extract, 10 parts of poncir trifoliata extract, 8 parts of red clover extract, 15 parts of papaya extract, 20 parts of royal jelly extract and 15 parts of wheat germ extract.

4. The moisturizing and skin-rejuvenating essence according to claim 1, which is characterized by comprising, in parts by weight: 35 parts of recombinant silk fibroin, 15 parts of white peony root extract, 15 parts of bletilla striata extract, 16 parts of purslane extract, 16 parts of honeysuckle extract, 20 parts of poncir trifoliata extract, 5 parts of red clover extract, 15 parts of papaya extract, 10 parts of royal jelly extract and 10 parts of wheat germ extract.

5. The moisturizing skin-rejuvenating lotion according to any one of claims 1 to 4, further comprising, in parts by weight: 1-1000 parts of solvent, 1-100 parts of thickener, 1-100 parts of humectant, 1-100 parts of skin conditioning agent, 1-100 parts of surfactant, 1-100 parts of chelating agent, 1-100 parts of softener, 1-100 parts of film forming agent, 1-100 parts of preservative and 1-100 parts of pH regulator.

6. A method for preparing the moisturizing and skin-tendering essence according to any one of claims 1 to 5, comprising the following steps:

s1: the recombinant fibroin prepared in the claim 1 is reserved;

s2: pulverizing radix Paeoniae alba, rhizoma Bletillae, herba Portulacae, flos Lonicerae, fructus Ponciri Trifoliatae, herba Trifoliatae, fructus Muscovitum, lac Regis Apis, and wheat embryo respectively, sieving, adding 10-20 times of solvent, soaking at 35-45deg.C for 7-9 hr, performing low temperature ultrasonic extraction for 5-7 hr, centrifuging, filtering to obtain supernatant, filtering again, lyophilizing to obtain corresponding plant extract, and mixing;

s4: adding the plant extract prepared in the step S2 into a container of the step S3, adding a surfactant, and heating and homogenizing at a rotating speed of 60-90rpm until the material is uniformly dispersed;

s5: adding the humectant into the S4, stirring and homogenizing, and keeping the vacuum state and continuing to keep the temperature and stirring for 10 minutes;

s6: cooling to 35-40deg.C, adding thickener and recombinant fibroin prepared in step S1 into the mixture prepared in step S5, and stirring;

7. The method for preparing the moisturizing and skin-tendering essence according to claim 6, wherein the stirring speed in S3 is 60-90rpm; the heat preservation stirring speed in the step S5 is 60-90rpm; the stirring speed in S6, S7 and S8 is 20-40rpm; the homogenization speed in S4 and S5 is 2000-3000rpm.

8. The method for preparing moisturizing and skin-tendering essence according to claim 6, wherein the solvent comprises at least one of purified water, butylene glycol, dipropylene glycol, propylene glycol, glycerin, and ethanol.

9. The method for preparing moisturizing and skin rejuvenating essence according to claim 6, wherein the skin conditioning agent comprises at least one of lysine, ascorbic acid, vitamin C, ceramide, magnesium ascorbyl phosphate, ascorbyl glucoside, ascorbyl palmitate, glutathione, dipotassium glycyrrhizinate, glabridin and lecithin.

10. The method for preparing moisturizing and skin-tendering essence according to claim 6, wherein the humectant comprises at least one of saccharide isomer, sodium hyaluronate, hydrolyzed sodium hyaluronate, 1, 2-hexanediol, sodium polyglutamate, squalane, acetylglucosamine, trehalose, urea, allantoin, polyether glycerol-26, sodium lactate, sodium pyrrolidone carboxylate, xylitol, and biogel-1.