CN113304088A - Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract - Google Patents
Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract Download PDFInfo
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- CN113304088A CN113304088A CN202110579366.9A CN202110579366A CN113304088A CN 113304088 A CN113304088 A CN 113304088A CN 202110579366 A CN202110579366 A CN 202110579366A CN 113304088 A CN113304088 A CN 113304088A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
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- Veterinary Medicine (AREA)
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- Biotechnology (AREA)
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- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Birds (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a bletilla striata extract with repairing and whitening effects and the bletilla striata extract. The method for preparing the bletilla striata extract provided by the invention utilizes technologies such as enzymolysis, resin adsorption, decoloration and the like, not only can remove unstable components in the bletilla striata, but also can retain an effective component of bletilla striata polysaccharide, and does not use a large amount of solvents such as ethanol, acetone, ether and the like in the preparation process, so that the processing cost is reduced, and the extraction yield is high; tests prove that the bletilla striata extract prepared by the method has whitening and repairing effects and can be applied to cosmetics; the bletilla striata extract prepared by the method is liquid and relatively stable.
Description
Technical Field
The invention relates to the technical field of plant extracts, in particular to a preparation method of a bletilla striata extract with repairing and whitening effects and the bletilla striata extract.
Background
Bletilla striata is a traditional and rare Chinese medicine and has the functions of detumescence, granulation promotion, convergence and hemostasis. Modern researches show that bletilla has the effects of resisting tumors, regulating immunity, promoting wound healing and the like.
Bletilla striata contains components such as starch, macromolecular protein, polyphenol and the like, and is easy to cause the problems of precipitation, color change and the like in a solution, so that the bletilla striata needs to be removed in the processing process so as to meet the requirement of cosmetics on the stability of products.
At present, the polysaccharide is mainly used for research and extraction, and technical means such as ethanol, acetone, ether, membrane filtration and the like are used for preparing and purifying the bletilla polysaccharide. Uses a large amount of organic solvent, brings potential safety hazard, or has the defects of high production cost and the like. The raw materials of cosmetics have high requirements on stability, safety and functionality, and the bletilla striata extract extracted in the prior art cannot be applied to the field of cosmetics.
Disclosure of Invention
In order to solve the problems in the prior art and meet the requirements of cosmetics, the invention provides a preparation method of a bletilla striata extract which does not use organic solvents, is convenient to operate, has high yield and low cost and has the effects of repairing and whitening;
the invention also provides a bletilla striata extract with repairing and whitening effects.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of bletilla striata extract with repairing and whitening effects comprises the following steps:
a) extraction: adding water 10-15 times the weight of rhizoma Bletillae into rhizoma Bletillae, extracting at 70-90 deg.C for 1-3 hr, and filtering to obtain crude filtrate;
b) enzymolysis: adding 0.05-5 wt% of acid protease into the coarse filtrate of bletilla striata, performing heat preservation enzymolysis for 1-6 hours, adding 0.05-5 wt% of high-temperature amylase after enzymolysis is finished, and performing heat preservation enzymolysis for 1-6 hours; after the enzymolysis is finished, adding a filter aid, and filtering to obtain a fine filtrate;
c) resin adsorption: adding ion exchange resin 4-15 wt% of the fine filtrate into the fine filtrate, treating for 1-6 hr, filtering, and adjusting pH of the filtrate to 5-7;
d) and (3) decoloring: adding a decoloring agent into the filtrate treated in the step c) for decoloring to obtain the bletilla extract.
Preferably, in the step b), before the addition of the acid protease, the temperature of the coarse filtrate is adjusted to 40-60 ℃, and the temperature for enzymolysis of the acid protease is 40-60 ℃; before adding the high-temperature amylase, the temperature of the acidic protease enzymolysis liquid is adjusted to 80-95 ℃, and the temperature of the high-temperature amylase enzymolysis liquid is 80-95 ℃.
Preferably, in the step b), before the addition of the acid protease, the temperature of the coarse filtrate is adjusted to 50-60 ℃, and the temperature for enzymolysis of the acid protease is 50-60 ℃; before adding the high-temperature amylase, the temperature of the acid protease zymolyte is adjusted to 85-95 ℃, and the temperature is 85-95 ℃ when the high-temperature amylase is used for enzymolysis.
Preferably, the filter aid in step b) is diatomaceous earth.
Preferably, in the step b), the filter aid is added in an amount of 4-15 wt% of the weight of the product after the high-temperature amylase enzymolysis.
Preferably, in the step b), the filter aid is added in an amount of 8-12 wt% of the weight of the product after the high-temperature amylase enzymolysis.
Preferably, in the step c), the addition amount of the ion exchange resin is 8-12 wt% of the weight of the fine filtrate, and the pH value is adjusted by using a hydrochloric acid solution.
Preferably, in the step d), the decoloring temperature is 50 to 70 ℃.
Preferably, in the step d), the decolorizing agent is activated carbon, and the adding amount of the decolorizing agent is 2-8 wt% of the weight of the filtrate.
A bletilla striata extract with repairing and whitening effects is prepared by the preparation method of the bletilla striata extract with repairing and whitening effects.
Therefore, the invention has the following beneficial effects:
1) the method for preparing the bletilla striata extract provided by the invention utilizes technologies such as enzymolysis, resin adsorption, decoloration and the like to prepare the bletilla striata extract, and not only can remove unstable components in bletilla striata, but also can retain an effective component bletilla striata polysaccharide.
2) The bletilla striata extract prepared by the invention not only obtains the active ingredient polysaccharide, but also does not use a large amount of solvents such as 95% ethanol, acetone, ether and the like in the preparation process, and has higher yield.
3) The bletilla striata extract prepared by the method is safe and non-irritant to skin, has no eye irritation, has whitening and repairing effects, and can be applied to cosmetics.
4) The bletilla striata extract prepared by the method is stable and does not precipitate.
Detailed Description
Embodiments of the present application will be described in detail by examples, so that how to apply technical means to solve technical problems and achieve technical effects of the present application can be fully understood and implemented.
The raw materials and equipment used in the present application are all common raw materials and equipment in the field, and are all from commercially available products, unless otherwise specified. The methods used in this application are conventional in the art unless otherwise indicated.
There are many other possible embodiments of the present invention, which are not listed here, and the embodiments claimed in the claims of the present invention can be implemented.
Examples
A preparation method of bletilla striata extract with repairing and whitening effects comprises the following steps:
a) extraction: adding water 10-15 times the weight of rhizoma Bletillae into rhizoma Bletillae, extracting at 70-90 deg.C for 1-3 hr, and filtering to obtain crude filtrate;
preferably, the weight of the added water is 11 to 13 times of that of the bletilla striata; more preferably, water is added to make the weight of the extract 12 times of the weight of rhizoma bletilla, and the extraction temperature is 80 deg.C;
b) enzymolysis: firstly, adjusting the temperature of coarse filtrate to 40-60 ℃, adding 0.05-5 wt% of acid protease into the coarse filtrate of bletilla striata, carrying out heat preservation enzymolysis for 1-6 hours at the temperature of 40-60 ℃, firstly adjusting the temperature to 80-95 ℃ after the enzymolysis is finished, adding 0.05-5 wt% of high-temperature amylase, and carrying out heat preservation enzymolysis for 1-6 hours at the temperature of 80-95 ℃; after the enzymolysis is finished, adding filter aid diatomite which accounts for 4-15 wt% of the weight of the product after the enzymolysis, and filtering to obtain a fine filtrate;
preferably, before adding the acid protease, the temperature of the rough filtrate is adjusted to 50-60 ℃, the temperature for enzymolysis of the acid protease is 50-60 ℃, before adding the high-temperature amylase, the temperature of the acid protease zymolyte is adjusted to 85-95 ℃, the temperature for enzymolysis of the high-temperature amylase is 85-95 ℃, and the addition amount of the filter aid is 8-12 wt% of the weight of the product after enzymolysis; more preferably, before adding the acid protease, the temperature of the rough filtrate is adjusted to 55 ℃, the temperature during enzymolysis of the acid protease is 55 ℃, before adding the high-temperature amylase, the temperature of the enzymolysis liquid of the acid protease is adjusted to 90 ℃, the temperature during enzymolysis of the high-temperature amylase is 90 ℃, and the addition amount of the filter aid is 10 wt% of the weight of the product after enzymolysis;
c) resin adsorption: adding ion exchange resin 4-15 wt% of the fine filtrate into the fine filtrate, treating for 1-6 hr, filtering, and adjusting pH of the filtrate to 5-7 with hydrochloric acid solution;
preferably, the addition amount of the ion exchange resin is 8-12 wt% of the weight of the fine filter liquid, and the pH value is adjusted to 6; more preferably, the addition amount of the ion exchange resin is 10 wt% of the weight of the fine filtrate, and the ion exchange resin is 717 resin;
d) and (3) decoloring: adding decolorant active carbon accounting for 2-8 wt% of the weight of the filtrate into the filtrate treated in the step c), and carrying out decoloration treatment for 1-3 hours at 50-70 ℃ to obtain the bletilla striata extract;
preferably, the decoloring treatment is carried out at 55-65 ℃, and the addition amount of the decoloring agent is 3-7 wt%; more preferably, the decolorization treatment is carried out at 55-65 ℃, and the addition amount of the decolorizer is 3-7 wt%.
A bletilla striata extract with repairing and whitening effects is prepared by the preparation method of the bletilla striata extract with repairing and whitening effects.
Example 1
Adding 150g of flaky bletilla striata into pure water which is 10 times of the weight of the raw materials, extracting for 3 hours at 70 ℃, filtering and removing slag while the solution is hot to obtain coarse bletilla striata filtrate, adjusting the temperature of the coarse filtrate to 60 ℃, adding 5 wt% of acid protease, preserving heat and performing enzymolysis for 1 hour, heating to 80 ℃ after the enzymolysis is finished, adding 5 wt% of high-temperature amylase, and preserving heat and performing enzymolysis for 1 hour. And after enzymolysis, adding 15 wt% of white diatomite into the enzymolysis liquid for filtration assistance to obtain a fine filtrate, adding ion exchange resin (preferably 717 resin) accounting for 15 wt% of the weight of the raw materials into the fine filtrate, stirring and adsorbing for 1 hour, filtering after adsorption, adding HCl into the filtrate, and adjusting the pH value to 6. Adjusting the pH of the filtrate to 70 deg.C, adding active carbon 8 wt% of the raw materials into the filtrate, stirring and decolorizing for 3 hr under heat preservation to obtain 1180g of rhizoma bletilla root extract product with solid content of 3.39%, and the filtrate is white to light yellow.
Example 2
Adding 150g of flaky bletilla striata into pure water which is 15 times of the weight of the raw materials, extracting for 1 hour at 90 ℃, filtering and removing slag while the solution is hot to obtain coarse bletilla striata filtrate, adjusting the temperature of the coarse filtrate to 40 ℃, adding 0.05 wt% of acid protease, preserving heat and performing enzymolysis for 6 hours, heating to 80 ℃ after the enzymolysis is finished, adding 0.05 wt% of high-temperature amylase, and preserving heat and performing enzymolysis for 6 hours. And after the enzymolysis is finished, adding 4 wt% of white diatomite into the enzymolysis liquid for filtration assistance to obtain a fine filtrate, adding ion exchange resin (preferably 717 resin) with the weight of 4 wt% of the raw material into the fine filtrate, stirring and adsorbing for 6 hours, filtering after the adsorption is finished, adding HCl into the filtrate, and adjusting the pH of the filtrate to be 6. Adjusting the pH of the filtrate to 50 deg.C, adding activated carbon 2 wt% of the raw materials into the filtrate, stirring and decolorizing for 1 hr under heat preservation to obtain 1324g of rhizoma bletilla root extract product with solid content of 3.3%, and the filtrate is white to light yellow.
Example 3
Adding 150g of flaky bletilla striata into pure water which is 12 times of the weight of the raw materials, extracting for 2 hours at 80 ℃, filtering and removing slag while the solution is hot to obtain coarse bletilla striata filtrate, adjusting the temperature of the coarse filtrate to 50 ℃, adding 2.5 wt% of acid protease, preserving heat and performing enzymolysis for 3 hours, heating to 90 ℃, adding 2.5 wt% of high-temperature amylase, preserving heat and performing enzymolysis for 3 hours. And after the enzymolysis is finished, adding 10 wt% of white diatomite into the enzymolysis liquid for assisting filtration to obtain a fine filtrate, adding ion exchange resin (preferably 717 resin) accounting for 10 wt% of the weight of the raw materials into the fine filtrate, stirring and adsorbing for 3 hours, filtering after adsorption is finished, adding HCl into the filtrate, and adjusting the pH value of the filtrate to be 6. Adjusting the pH of the filtrate to 60 deg.C, adding 6 wt% active carbon into the filtrate, stirring and decolorizing for 2 hr to obtain 1218g rhizoma bletilla extract product with solid content of 3.1%, and white to light yellow filtrate.
Example 4
Adding 150g of flaky bletilla striata into pure water 13 times of the weight of the raw materials, extracting for 2 hours at 90 ℃, finishing extraction, filtering and removing slag while the mixture is hot to obtain coarse bletilla striata filtrate, adjusting the temperature of the coarse filtrate to 55 ℃, adding 0.5 wt% of acid protease, preserving heat and performing enzymolysis for 2 hours, finishing enzymolysis, heating to 95 ℃, adding 0.5 wt% of high-temperature amylase, and preserving heat and performing enzymolysis for 2 hours. And after the enzymolysis is finished, adding 8 wt% of white diatomite into the enzymolysis liquid for filtration assistance to obtain a fine filtrate, adding ion exchange resin (preferably 717 resin) accounting for 5 wt% of the weight of the raw materials into the fine filtrate, stirring and adsorbing for 2 hours, filtering after the adsorption is finished, adding HCl into the filtrate, and adjusting the pH value of the filtrate to be 6. Adjusting the pH of the filtrate to 60 deg.C, adding 6 wt% active carbon into the filtrate, stirring and decolorizing for 1 hr to obtain 1250g rhizoma bletilla root extract product with solid content of 3.2%, and white to light yellow filtrate.
Example 5
Adding 150g of flaky bletilla striata into pure water which is 15 times of the weight of the raw materials, extracting for 3 hours at 80 ℃, filtering and removing slag while the solution is hot to obtain coarse bletilla striata filtrate, adjusting the temperature of the coarse filtrate to 50 ℃, adding 3 wt% of acid protease, preserving heat and performing enzymolysis for 3 hours, heating to 95 ℃ after the enzymolysis is finished, adding 3 wt% of high-temperature amylase, and preserving heat and performing enzymolysis for 3 hours. And after the enzymolysis is finished, adding 10 wt% of white diatomite into the enzymolysis liquid for assisting filtration to obtain a fine filtrate, adding ion exchange resin (preferably 717 resin) accounting for 10 wt% of the weight of the raw materials into the fine filtrate, stirring and adsorbing for 3 hours, filtering after adsorption is finished, adding HCl into the filtrate, and adjusting the pH value of the filtrate to be 6. Adjusting the pH of the filtrate to 70 deg.C, adding 6 wt% active carbon into the filtrate, stirring and decolorizing for 2 hr to obtain 1350g rhizoma bletilla root extract product with solid content of 3.6%, and white to light yellow filtrate.
Comparative example 1
The preparation method in comparative example 1 was the same as that of example 2 except that the ion exchange resin adsorption was not performed.
Comparative example 2
The preparation method in comparative example 2 was the same as that of example 2 except that the ion exchange resin adsorption and the decoloring treatment were not performed.
Comparative example 3
The preparation method in comparative example 3 was the same as that of example 2 except that the treatment order of acid protease and high temperature amylase was changed.
Comparative example 4
The preparation method of comparative example 4 was the same as that of example 2 except that the extraction temperature was 100 ℃.
Comparative example 5
The preparation method of comparative example 5 was the same as that of example 2 except that the extraction temperature was 60 ℃.
Stability test method: and (3) placing the sample in a constant-temperature drying oven at 60 ℃ and 80 ℃, observing whether the sample is precipitated or not, and comparing the sample with the initial sample. The test was carried out for 10 days, and the product was observed on day 5 and day 10, respectively. The results are shown in Table 1.
TABLE 1 bletilla striata root extract test results obtained with different methods
Immunoregulation effect of bletilla striata root extract on keratinocyte HACAT
Cell collection: when the keratinocyte plating rate reaches 60-75%, the culture solution in the culture bottle is discarded, PBS is used for cleaning twice, 1mL of pancreatin digestive cells with the concentration of 0.25% is added, the cells are digested for 3-5min at 37 ℃, 5mL of DMEM culture solution containing serum is added to stop the digestion when about 80% of the cells are in a suspension state under an inverted microscope, the cells in the culture bottle are collected in a centrifuge tube, and the cells are centrifuged for 3min at 1000 rpm/min.
Cell counting: after centrifugation, the supernatant was discarded, 5mL of PBS was added, the cells were flushed through an elbow pipette and counted in a cell counter.
Cell inoculation: after centrifugation at 1000rpm for 3min to discard PBS, the cell suspension was seeded into 12-well plates at a volume of 1mL per well. After completion of the inoculation, the cells were placed in an incubator (37 ℃ C., 5% CO)295% RH) for 24h +/-2 h.
Grouping experiments: the experiment required setting blank control group (no LPS induction), negative control group (LPS induction), sample group (LPS induction) and positive control group (LPS induction + dexamethasone 100 μ g/mL), each group set up 3 multiple wells.
Sample preparation: then diluted with cell culture medium to the corresponding concentration (0.5%, 0.1%).
Administration: according to experimental grouping, drug administration operation is carried out, a sample group is added with culture solution containing a sample, a blank control group and a negative control group are added with cell culture solution, and a positive control group is added with cell culture solution containing 100 mu g/mL dexamethasone, wherein each well is 2 mL. After the administration, the 6-well plate was placed in an incubator (37 ℃, 5% CO2, 95% RH) and incubated for 2 h. + -. 15 min.
And (3) LPS induction: after the incubation culture is finished, replacing fresh cell culture solution for a blank control group, replacing LPS culture solution containing 1 mu g/mL for the rest groups, adding cell culture solution containing LPS into a negative control group, adding culture solution containing a sample and LPS into a sample group, and adding cell culture solution containing 100 mu g/mL dexamethasone and LPS into a positive control group, wherein each well is 2 mL. After the administration, the 6-well plate was placed in an incubator (37 ℃, 5% CO2, 95% RH) and incubated for 22 h. + -. 1 h.
Collecting cell supernatant: after the incubation culture is finished, collecting cell culture supernatant into a 1.5mL centrifuge tube, and placing the centrifuge tube in an ultra-low temperature refrigerator at-80 ℃ for freezing and storing.
Detecting the content of IL-1 beta, IL-6, IL-8 and TNF-alpha: detection was performed according to the instructions of the test kit.
The bletilla striata root extract can reduce the inflammatory reaction of cells, thereby improving the immunologic function of skin and leading the skin to have better effect of resisting the environment to the skin. Specific results are shown in table 2.
TABLE 2
Similarly, the bletilla striata root extract obtained according to method 2, method 3, method 4 and method 5 in example 1 also had a repairing effect.
The details not described in the specification of the present application belong to the common general knowledge of those skilled in the art.
In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. "substantially" means within an acceptable error range, and a person skilled in the art can solve the technical problem within a certain error range to substantially achieve the technical effect.
It is also noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a good or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such good or system. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a commodity or system that includes the element.
The foregoing description shows and describes several preferred embodiments of the present application, but as aforementioned, it is to be understood that the application is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the application, which is to be protected by the claims appended hereto.
Claims (10)
1. A preparation method of bletilla striata extract with repairing and whitening effects is characterized by comprising the following steps:
a) extraction: adding water 10-15 times the weight of rhizoma Bletillae into rhizoma Bletillae, extracting at 70-90 deg.C for 1-3 hr, and filtering to obtain crude filtrate;
b) enzymolysis: adding 0.05-5 wt% of acid protease into the coarse filtrate of bletilla striata, performing heat preservation enzymolysis for 1-6 hours, adding 0.05-5 wt% of high-temperature amylase after enzymolysis is finished, and performing heat preservation enzymolysis for 1-6 hours; after the enzymolysis is finished, adding a filter aid, and filtering to obtain a fine filtrate;
c) resin adsorption: adding ion exchange resin 4-15 wt% of the fine filtrate into the fine filtrate, treating for 1-6 hr, filtering, and adjusting pH of the filtrate to 5-7;
d) and (3) decoloring: adding a decoloring agent into the filtrate treated in the step c) for decoloring to obtain the bletilla extract.
2. The method of claim 1, wherein:
the bletilla striata in the step a) is flaky;
in the step b), before adding the acid protease, the temperature of the coarse filtrate is adjusted to 40-60 ℃, and the temperature is 40-60 ℃ when the acid protease is used for enzymolysis; before adding the high-temperature amylase, the temperature of the acidic protease enzymolysis liquid is adjusted to 80-95 ℃, and the temperature of the high-temperature amylase enzymolysis liquid is 80-95 ℃.
3. The method of claim 2, wherein:
in the step b), before adding the acid protease, the temperature of the coarse filtrate is adjusted to 50-60 ℃, and the temperature is 50-60 ℃ when the acid protease is used for enzymolysis; before adding the high-temperature amylase, the temperature of the acid protease zymolyte is adjusted to 85-95 ℃, and the temperature is 85-95 ℃ when the high-temperature amylase is used for enzymolysis.
4. The method of claim 2, wherein:
the filter aid in the step b) is diatomite.
5. The method of claim 2, wherein:
in the step b), the addition amount of the filter aid is 4-15 wt% of the weight of the product after the enzymolysis of the high-temperature amylase.
6. The method of claim 5, wherein:
in the step b), the addition amount of the filter aid is 8-12 wt% of the weight of the product after the enzymolysis of the high-temperature amylase.
7. The method of claim 2, wherein:
in the step c), the addition amount of the ion exchange resin is 8-12 wt% of the weight of the fine filtrate, and the pH value is adjusted by adopting a hydrochloric acid solution.
8. The method of claim 2, wherein:
in the step d), the decoloring temperature is 50-70 ℃.
9. The method of claim 2, wherein:
in the step d), the decolorizing agent is activated carbon, and the adding amount of the decolorizing agent is 2-8 wt% of the weight of the filtrate.
10. A bletilla striata extract with repairing and whitening effects, which is characterized by being prepared by the preparation method of any one of claims 1-9.
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