CN113288856A - A toothpaste for preventing and treating dental caries and periodontitis and eliminating oral odor - Google Patents
A toothpaste for preventing and treating dental caries and periodontitis and eliminating oral odor Download PDFInfo
- Publication number
- CN113288856A CN113288856A CN202110491393.0A CN202110491393A CN113288856A CN 113288856 A CN113288856 A CN 113288856A CN 202110491393 A CN202110491393 A CN 202110491393A CN 113288856 A CN113288856 A CN 113288856A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus salivarius
- toothpaste
- sodium
- extract
- toothpaste according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000000606 toothpaste Substances 0.000 title claims abstract description 46
- 229940034610 toothpaste Drugs 0.000 title claims abstract description 44
- 201000001245 periodontitis Diseases 0.000 title claims abstract description 15
- 208000002925 dental caries Diseases 0.000 title abstract description 17
- 241000186869 Lactobacillus salivarius Species 0.000 claims abstract description 32
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 210000001808 exosome Anatomy 0.000 claims abstract description 24
- 239000004475 Arginine Substances 0.000 claims abstract description 15
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 15
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 13
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 13
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims abstract description 13
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 13
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 13
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004202 carbamide Substances 0.000 claims abstract description 13
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 13
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 13
- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 13
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 13
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 13
- 239000000600 sorbitol Substances 0.000 claims abstract description 13
- 239000000811 xylitol Substances 0.000 claims abstract description 13
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 13
- 229960002675 xylitol Drugs 0.000 claims abstract description 13
- 235000010447 xylitol Nutrition 0.000 claims abstract description 13
- 239000008223 sterile water Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 241001052560 Thallis Species 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 7
- 239000008363 phosphate buffer Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 229920000136 polysorbate Polymers 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000008055 phosphate buffer solution Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 241000186660 Lactobacillus Species 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 229940039696 lactobacillus Drugs 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 21
- 210000000214 mouth Anatomy 0.000 abstract description 19
- 230000005802 health problem Effects 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 201000010099 disease Diseases 0.000 description 8
- 239000000523 sample Substances 0.000 description 7
- 208000028169 periodontal disease Diseases 0.000 description 6
- 230000001680 brushing effect Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 230000003902 lesion Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000003239 periodontal effect Effects 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 241000186046 Actinomyces Species 0.000 description 2
- 206010006326 Breath odour Diseases 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 241000736262 Microbiota Species 0.000 description 2
- 208000008312 Tooth Loss Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000001013 cariogenic effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 241000186044 Actinomyces viscosus Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000002064 Dental Plaque Diseases 0.000 description 1
- 208000027244 Dysbiosis Diseases 0.000 description 1
- 241000605986 Fusobacterium nucleatum Species 0.000 description 1
- 208000032139 Halitosis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000005888 Periodontal Pocket Diseases 0.000 description 1
- 206010034535 Periodontal destruction Diseases 0.000 description 1
- 241000605862 Porphyromonas gingivalis Species 0.000 description 1
- 241000605861 Prevotella Species 0.000 description 1
- 241001135221 Prevotella intermedia Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000589892 Treponema denticola Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical group 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 201000011529 cardiovascular cancer Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 230000006020 chronic inflammation Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000005115 demineralization Methods 0.000 description 1
- 230000002328 demineralizing effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007140 dysbiosis Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000004195 gingiva Anatomy 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 210000002379 periodontal ligament Anatomy 0.000 description 1
- 239000003761 preservation solution Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cosmetics (AREA)
Abstract
The invention provides toothpaste for preventing and treating decayed teeth and periodontitis and eliminating oral peculiar smell, which comprises the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea and one or more of the following components: 0.3-15% of lactobacillus salivarius extract, 0.3-15% of lactobacillus salivarius exosome, 1-20% of arginine and the balance of sterile water. The toothpaste provided by the invention adopts lactobacillus salivarius extract or lactobacillus salivarius exosomes and arginine, can obviously inhibit the quantity of harmful bacteria, improves the environment in the oral cavity, can effectively prevent and treat oral health problems such as tooth decay and periodontitis, and can eliminate oral peculiar smell.
Description
Technical Field
The invention relates to the field of toothpaste production processes, in particular to toothpaste for preventing and treating decayed teeth and periodontitis and eliminating oral peculiar smell.
Background
The oral microbiota contains more than 700 microorganisms, most of the bacteria are normal oral flora, only a few bacteria are closely related to the occurrence and development of periodontal diseases such as tooth decay, periodontitis and the like, in lesion areas of various types of periodontal diseases, one or more dominant bacteria can be isolated, have obvious virulence or pathogenicity, can interfere with host defense capacity through multiple mechanisms, have the potential of triggering periodontal destruction, and are called as oral harmful bacteria.
The harmful bacteria in the oral cavity are the main factors of periodontal disease and also are important factors influencing the health of the oral cavity. Oral microbiota is a key factor in health or disease. Periodontal disease causing ecological disorder of oral cavity microorganism, which can cause oral cavity ecosystem disorder and systemic disease; in addition, dysbiosis of the oral cavity is associated with systemic diseases such as cardiovascular diseases, cancer and diabetes.
(1) Cariogenic bacteria and tooth decay:
dental caries, the name dental caries, is a typical bacterial disease; the oral cavity harmful bacteria: the modified streptococcus and actinomyces are attached to the surface of teeth for a long time to form dental plaque which becomes dominant flora in the oral cavity and the periodontal; the decomposition of carbohydrates in the mouth produces a large amount of acid, which attacks the teeth. If the treatment and prevention are not timely performed, the lesion continues to develop to form a cavity, and finally the dental crown is completely destroyed.
Untreated caries cavities do not heal on their own and the end result of their development is tooth loss. Dental caries is a progressive lesion of hard tooth tissue caused by the combined action of various factors in the oral cavity, and is represented by the evolution process of inorganic demineralization and organic matter decomposition from color change to substantial lesion formation along with the course of disease development. It features high incidence rate and wide distribution.
Dental caries is a main common disease of the oral cavity and is one of the most common diseases of human beings, and the world health organization combines the dental caries with cancer and cardiovascular diseases as three major prevention and treatment diseases of human beings.
(2) Periodontitis
Periodontitis, also known as destructive periodontal disease, is a chronic inflammation caused by the invasion of periodontal tissues by periodontal bacteria, which can lead to the destruction of periodontal supporting tissues (gingiva, periodontal ligament, alveolar bone and cementum); periodontal pocket formation, loss of attachment and alveolar bone dilution, as the disease progresses, the teeth slowly loosen and the gums recede, eventually leading to tooth loss. Represents a bacterium: actinomyces, Porphyromonas gingivalis, Fostanemia, Fusobacterium nucleatum, Prevotella intermedia, Treponema denticola, Prevotella nigricans, Actinomyces viscosus.
Harmful bacteria in the oral cavity exist for a long time, which causes serious periodontal diseases, bad breath and halitosis. More importantly, the harmful bacteria in the oral cavity are rooted for a long time, which affects the brain-intestinal axis development of children and causes irreversible influence. Moreover, no effective treatment medicine exists for the oral problems of children. The selection of the right toothpaste, the right brushing of the teeth is the most effective solution for preventing and treating oral problems.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides toothpaste for preventing and treating tooth decay and periodontitis and eliminating oral peculiar smell.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides toothpaste for preventing and treating decayed teeth and periodontitis and eliminating oral peculiar smell, which comprises the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol and 0.3-10% of urea; also comprises one or more selected from the following: 0.3-15% of lactobacillus salivarius extract, 0.3-15% of lactobacillus salivarius exosome and 1-20% of arginine; the balance being sterile water.
Further, the toothpaste comprises the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea, 1-20% of arginine and the balance of sterile water.
Further, the toothpaste comprises the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea, 0.3-15% of lactobacillus salivarius extract or lactobacillus salivarius exosome, and the balance of sterile water.
Further, the toothpaste comprises the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea, 1-20% of arginine, 0.3-15% of lactobacillus salivarius extract or lactobacillus salivarius exosomes, and the balance of sterile water.
Further, the preparation method of the lactobacillus salivarius extract comprises the following steps:
step one, collecting cultured thalli and fermentation liquor, centrifuging and collecting thalli;
step two, adding phosphate buffer solution and MgSO (MgSO) into the thallus4And Tween 2.0, homogenizing at high speed and fully crushing the thalli;
and step three, centrifuging at low temperature to collect supernatant, adding CMC, and freeze-drying to prepare the lactobacillus extract freeze-dried powder.
Further, the culture medium used for culturing in the first step comprises: 13g/L glucose, 21g/L peptone, 5.5g/L yeast powder, 2.0g/L dipotassium hydrogen phosphate, 9g/L sodium acetate, 0.6g/L magnesium sulfate and 0.07g/L manganese sulfate.
Further, the pH of the phosphate buffer used in step two was 6.5.
Furthermore, the addition amount of the phosphate buffer solution in the second step is equivalent to 10 times of the cell volume; MgSO (MgSO)4To a final concentration of 2 mM; the final concentration of Tween 2.0 was 0.1%.
Further, the preparation method of the lactobacillus salivarius exosomes comprises the steps of centrifuging the culture medium after fermentation culture at low temperature, and collecting the supernatant; centrifuging the supernatant at low temperature, and collecting the precipitate to obtain the lactobacillus salivarius exosome.
Further, the lactobacillus salivarius exosomes are dissolved in a phosphate buffer for later use.
By adopting the technical scheme, compared with the prior art, the invention has the following technical effects:
the toothpaste provided by the invention adopts lactobacillus salivarius extract or lactobacillus salivarius exosomes and arginine, can obviously inhibit the quantity of harmful bacteria, improves the environment in the oral cavity, can effectively prevent and treat oral health problems such as tooth decay and periodontitis, and can eliminate oral peculiar smell.
Drawings
FIG. 1 is a statistical chart showing the number of harmful bacteria in the oral cavity before and after a subject uses toothpaste according to the present invention by using fluorescence quantitative PCR; wherein, the Ct value is low, and the content of harmful bacteria in the oral cavity is higher; high Ct value and low bacteria content.
Detailed Description
The invention provides toothpaste for preventing and treating decayed teeth and periodontitis and eliminating oral peculiar smell, which comprises the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol and 0.3-10% of urea; also comprises one or more selected from the following: 0.3-15% of lactobacillus salivarius extract, 0.3-15% of lactobacillus salivarius exosome and 1-20% of arginine; the balance being sterile water.
In a preferred embodiment of the present invention, the method for preparing the lactobacillus salivarius extract comprises the following steps:
1) fermentation culture: the culture conditions are as follows: 13g/L glucose, 21g/L peptone, 5.5g/L yeast powder, 2.0g/L dipotassium hydrogen phosphate, 9g/L sodium acetate, 0.6g/L magnesium sulfate and 0.07g/L manganese sulfate; the fermentation condition is pH value 6.5, the inoculum size is 4%, and the mixture is statically cultured for 24 hours at 37 ℃; the viable count is improved by nearly 4.0 multiplied by 10 after the fermentation condition is optimized9collecting the thallus and the fermentation liquid at cfu/mL.
2) And (3) collecting thalli: 1800g was centrifuged at low temperature (4 ℃) for 20 minutes to collect cells.
3) And (3) crushing thalli: 10 volumes of phosphate buffer (pH 6.5) were added to the reaction mixture to a final concentration of 2mM MgSO40.1% Tween 2.0, 1800g high speed homogenizing for 5 minutes to break the thallus fully.
4) Extracting: after the homogenate was disrupted, 1000g of the homogenate was centrifuged at low temperature (4 ℃) for 10 minutes, and the supernatant was collected. The supernatant was lyophilized after adding 0.2% CMC. Freeze-drying conditions: the thickness of the supernatant is 4-6 mm; and (5) freeze-drying and collecting dry powder.
In a preferred embodiment of the present invention, the preparation method of lactobacillus salivarius exosomes comprises the following steps:
1) centrifuging the culture medium after fermentation culture at 1800g low temperature (4 ℃) for 20 minutes, and collecting the supernatant;
2) collecting exosomes: 12000g, centrifuging for 20 minutes at low temperature (4 ℃), collecting the precipitate to obtain an exosome; phosphate buffer (MgSO 2mM final concentration) was added at 10X weight ratio4) And dissolving for later use.
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
Comparative example 1
The present comparative example provides a toothpaste containing unbroken bacterial cells having the following formulation:
TABLE 1 formulation information of toothpaste containing non-disrupted bacterial cells (toothpaste 1)
Example 1
This example provides five preferred toothpastes according to the invention, formulated as follows from tables 2 to 6:
table 2 toothpaste 2 formula information
| Serial number | Toothpaste 2 (arginine) | Component (A) |
| 1 | Xylitol, its preparation method and use | 10％ |
| 2 | Polyethylene glycol | 2.5％ |
| 3 | Sodium carboxymethylcellulose | 0.25％ |
| 4 | Essence | 1.0％ |
| 5 | Sodium lauryl sulfate | 1.0％ |
| 6 | Silicon dioxide | 2.0％ |
| 7 | Sorbitol | 10％ |
| 8 | Urea | 1.5％ |
| 9 | Arginine | 8％ |
TABLE 3 formulation information for toothpaste 3
Table 4 toothpaste 4 formula information
| Serial number | Toothpaste 4 (arginine + exosome) | Component (A) |
| 1 | Xylitol, its preparation method and use | 10％ |
| 2 | Polyethylene glycol | 2.5％ |
| 3 | Sodium carboxymethylcellulose | 0.25％ |
| 4 | Essence | 1.0％ |
| 5 | Sodium lauryl sulfate | 1.0％ |
| 6 | Silicon dioxide | 2.0％ |
| 7 | Sorbitol | 10％ |
| 8 | Urea | 1.5％ |
| 9 | Lactobacillus salivarius (exosome) | 10％ |
| 10 | Arginine | 8％ |
TABLE 5 formulation information for toothpaste 5
TABLE 6 toothpaste 6 recipe information
| Serial number | Toothpaste 6 (exosome) | Component (A) |
| 1 | Xylitol, its preparation method and use | 10％ |
| 2 | Polyethylene glycol | 2.5％ |
| 3 | Sodium carboxymethylcellulose | 0.25％ |
| 4 | Essence | 1.0％ |
| 5 | Sodium lauryl sulfate | 1.0％ |
| 6 | Silicon dioxide | 2.0％ |
| 7 | Sorbitol | 10％ |
| 8 | Urea | 1.5％ |
| 9 | Lactobacillus salivarius (exosome) | 10％ |
Verification examples
In this example, 120 subjects with periodontitis and tooth decay were grouped into 6 groups according to the degree of tooth decay and periodontitis as close as possible, and the teeth were brushed with the above-mentioned toothpastes: brushing teeth three times a day; the toothbrush is consistent when used in the morning, the noon and the evening; before and after the corresponding toothpaste is adopted for tooth brushing, the fluorescent PCR is used for detecting the harmful microorganisms in the oral cavity, and the specific operation steps and results are as follows:
step one, collecting saliva of a subject by using a saliva collector before brushing teeth in the morning at 0 week, 1 week, 2 week and 4 week; at the same time, a plaque sample was collected with a sterile collection brush. After brushing the surface of the teeth for 3-5 times, placing the sterilized toothbrush in a preservation solution; simultaneously gargling with sterile water and collecting the gargle;
step two, detecting the collected sample:
(1) primers and probes are shown in Table 7.
Reference 1: rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodogenic Bacteria.
Reference 2: multiplex real-time PCR detection and relative qualification of periodic Pathologes.
TABLE 7 primer and Probe sequence information
(2) PCR system and conditions: see the literature.
(3) And (4) judging a result:
FAM/Cy 5: ct value is less than 20, the bacterial load is very high, and tooth decay is obvious;
21< Ct value <30, high bacterial load, and visible tooth decay process;
31< Ct value <34, mild or asymptomatic;
ROX: ct value <25, invalid experiment, re-experiment.
(4) Sample treatment: a500. mu.l sample was taken and nucleic acid was extracted using a nucleic acid extraction instrument.
(5) Sample detection: 10 microliter of the extracted nucleic acid was sampled and tested.
As shown in fig. 1, the detection results are as follows:
toothpaste 1 is not effective in improving the number of harmful bacteria in the oral cavity, and is basically ineffective;
the toothpaste 2 can obviously suppress harmful bacteria, and the quantity is obviously reduced; in the fourth week, equilibrium is substantially maintained, but it cannot be suppressed to a low level;
the toothpaste 5 can effectively suppress harmful bacteria, the quantity is obviously reduced gradually, but the toothpaste cannot be completely suppressed;
the effect of toothpaste 6 is better than that of arginine and extract, the quantity is obviously reduced and gradually reduced;
the effect of the toothpaste 3 is very obvious, after the third week, the bacterial number is basically close to the critical point, and the reduction rate has no exosome and has good effect;
toothpaste 4 is the best group, can suppress harmful bacteria rapidly, improves the environment in the oral cavity.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. It will be appreciated by those skilled in the art that any equivalent modifications and substitutions are within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (10)
1. The toothpaste for preventing and treating decayed teeth and periodontitis and eliminating oral peculiar smell is characterized by comprising the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol and 0.3-10% of urea; also comprises one or more selected from the following: 0.3-15% of lactobacillus salivarius extract, 0.3-15% of lactobacillus salivarius exosome and 1-20% of arginine; the balance being sterile water.
2. The toothpaste according to claim 1, comprising the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea, 1-20% of arginine and the balance of sterile water.
3. The toothpaste according to claim 1, comprising the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea, 0.3-15% of lactobacillus salivarius extract or lactobacillus salivarius exosome, and the balance of sterile water.
4. The toothpaste according to claim 1, comprising the following components in percentage by mass: 5-30% of xylitol, 0.5-5% of polyethylene glycol, 0.1-0.5% of sodium carboxymethylcellulose, 0.1-1.0% of essence, 0.1-5% of sodium dodecanol sulfate, 0.2-5.0% of silicon dioxide, 1-20% of sorbitol, 0.3-10% of urea, 1-20% of arginine, 0.3-15% of lactobacillus salivarius extract or lactobacillus salivarius exosomes, and the balance of sterile water.
5. The toothpaste according to claim 1, wherein in step 2, the preparation method of the lactobacillus salivarius extract comprises the following steps:
step one, collecting cultured thalli and fermentation liquor, centrifuging and collecting thalli;
step two, adding phosphate buffer solution and MgSO (MgSO) into the thallus4And Tween 2.0, homogenizing at high speed and fully crushing the thalli;
and step three, centrifuging at low temperature to collect supernatant, adding CMC, and freeze-drying to prepare the lactobacillus extract freeze-dried powder.
6. The toothpaste of claim 5, wherein the culture medium used in the first culturing step comprises: 13g/L glucose, 21g/L peptone, 5.5g/L yeast powder, 2.0g/L dipotassium hydrogen phosphate, 9g/L sodium acetate, 0.6g/L magnesium sulfate and 0.07g/L manganese sulfate.
7. The toothpaste according to claim 5, wherein the phosphate buffer used in step two has a pH of 6.5.
8. The toothpaste according to claim 5, wherein the phosphate buffer is added in an amount corresponding to 10 times the volume of the bacterial cells in the second step; MgSO (MgSO)4To a final concentration of 2 mM; the final concentration of Tween 2.0 was 0.1%.
9. The toothpaste according to claim 1, wherein the lactobacillus salivarius exosomes are prepared by low-temperature centrifugation of a culture medium after fermentation culture, and collection of a supernatant; centrifuging the supernatant at low temperature, and collecting the precipitate to obtain the lactobacillus salivarius exosome.
10. The toothpaste according to claim 9, wherein the lactobacillus salivarius exosomes are dissolved in a phosphate buffer for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110491393.0A CN113288856A (en) | 2021-05-06 | 2021-05-06 | A toothpaste for preventing and treating dental caries and periodontitis and eliminating oral odor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110491393.0A CN113288856A (en) | 2021-05-06 | 2021-05-06 | A toothpaste for preventing and treating dental caries and periodontitis and eliminating oral odor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113288856A true CN113288856A (en) | 2021-08-24 |
Family
ID=77320935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110491393.0A Withdrawn CN113288856A (en) | 2021-05-06 | 2021-05-06 | A toothpaste for preventing and treating dental caries and periodontitis and eliminating oral odor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113288856A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115040465A (en) * | 2022-07-06 | 2022-09-13 | 浙江爱尚日用品有限公司 | Whitening toothpaste capable of effectively removing dental plaque and tartar |
-
2021
- 2021-05-06 CN CN202110491393.0A patent/CN113288856A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115040465A (en) * | 2022-07-06 | 2022-09-13 | 浙江爱尚日用品有限公司 | Whitening toothpaste capable of effectively removing dental plaque and tartar |
| CN115040465B (en) * | 2022-07-06 | 2023-01-24 | 浙江爱尚日用品有限公司 | Whitening toothpaste capable of effectively removing dental plaque and tartar |
| US11771644B1 (en) | 2022-07-06 | 2023-10-03 | Zhejiang Airsun Commodity Co., Ltd. | Whitening toothpaste capable of effectively removing dental plaque and tartar |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Oguntebi et al. | Predominant microflora associated with human dental periapical abscesses | |
| Dahlén et al. | The effect of supragingival plaque control on the subgingival microbiota in subjects with periodontal disease | |
| Krasse et al. | Implantation of caries-inducing streptococci in the human oral cavity | |
| Sundqvist et al. | Prevalence of black-pigmented bacteroides species in root canal infections | |
| Slots et al. | The occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults | |
| Van Winkelhoff et al. | Bacteroides endodontalis and other black-pigmented Bacteroides species in odontogenic abscesses | |
| Socransky et al. | Effect of therapy on periodontal infections | |
| Syed et al. | Predominant cultivable flora isolated from human root surface caries plaque | |
| Waltimo et al. | Fungi in therapy‐resistant apical periodontitis | |
| Fukushima et al. | Localization and identification of root canal bacteria in clinically asymptomatic periapical pathosis | |
| Gomes et al. | Clinical significance of dental root canal microflora | |
| Tennert et al. | New bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings | |
| Bjørndal et al. | A clinical and microbiological study of deep carious lesions during stepwise excavation using long treatment intervals | |
| Molander et al. | The antimicrobial effect of calcium hydroxide in root canals pretreated with 5% iodine potassium iodide | |
| Giuliana et al. | Occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings | |
| Weiger et al. | Microbial flora of sinus tracts and root canals of non‐vital teeth | |
| Cortelli et al. | Detection of Actinobacillus actinomycetemcomitans in unstimulated saliva of patients with chronic periodontitis | |
| Reit et al. | The diagnostic accuracy of microbiologic root canal sampling and the influence of antimicrobial dressings | |
| CN113288856A (en) | A toothpaste for preventing and treating dental caries and periodontitis and eliminating oral odor | |
| Sahin et al. | Antibacterial efficacy and remineralization capacity of glycyrrhizic acid added casein phosphopeptide‐amorphous calcium phosphate | |
| D'Angelo et al. | Treatment of prepubertal periodontitis: a case report and discussion | |
| DOHUDE et al. | Inhibitory test of turmeric leaves extract (Curcuma Longa. L) against the growth of Streptococcus mutans bacterial growth in vitro | |
| Ghahramani et al. | Time-dependent antibacterial effects of Citrullus colocynthis seed extract compared to calcium hydroxide in teeth infected with Enterococcus faecalis | |
| CN113101243B (en) | Oral soft tissue cell repair promoter, oral care composition and application | |
| Skaar et al. | A follow‐up case report of Actinobacillus actinomycetemcomitans in human periodontal disease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210824 |