CN113267492A - Based on CeO2Nano-rod urine glucose test paper and preparation method thereof - Google Patents
Based on CeO2Nano-rod urine glucose test paper and preparation method thereof Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 86
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 40
- 239000008103 glucose Substances 0.000 title claims abstract description 40
- 210000002700 urine Anatomy 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 239000002073 nanorod Substances 0.000 claims abstract description 48
- 239000000725 suspension Substances 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 15
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 12
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- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 12
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 12
- 238000001027 hydrothermal synthesis Methods 0.000 claims abstract description 10
- 239000002244 precipitate Substances 0.000 claims abstract description 9
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- 150000000703 Cerium Chemical class 0.000 claims abstract description 8
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- 238000000034 method Methods 0.000 claims description 14
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- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 11
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 claims description 5
- HSJPMRKMPBAUAU-UHFFFAOYSA-N cerium(3+);trinitrate Chemical compound [Ce+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O HSJPMRKMPBAUAU-UHFFFAOYSA-N 0.000 claims description 4
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- VYLVYHXQOHJDJL-UHFFFAOYSA-K cerium trichloride Chemical compound Cl[Ce](Cl)Cl VYLVYHXQOHJDJL-UHFFFAOYSA-K 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- ZBQCCTCQUCOXBO-UHFFFAOYSA-N 4-(4-aminophenyl)-2,2,6,6-tetramethylcyclohex-3-en-1-amine Chemical compound CC1(C)C(N)C(C)(C)CC(C=2C=CC(N)=CC=2)=C1 ZBQCCTCQUCOXBO-UHFFFAOYSA-N 0.000 claims description 2
- OZECDDHOAMNMQI-UHFFFAOYSA-H cerium(3+);trisulfate Chemical compound [Ce+3].[Ce+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O OZECDDHOAMNMQI-UHFFFAOYSA-H 0.000 claims description 2
- 239000003086 colorant Substances 0.000 claims description 2
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 238000009535 clinical urine test Methods 0.000 claims 1
- 229910000422 cerium(IV) oxide Inorganic materials 0.000 abstract description 26
- 239000002086 nanomaterial Substances 0.000 abstract description 12
- 238000001514 detection method Methods 0.000 abstract description 6
- CETPSERCERDGAM-UHFFFAOYSA-N ceric oxide Chemical compound O=[Ce]=O CETPSERCERDGAM-UHFFFAOYSA-N 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 18
- 229940088598 enzyme Drugs 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 238000005303 weighing Methods 0.000 description 6
- 230000003197 catalytic effect Effects 0.000 description 5
- 239000000446 fuel Substances 0.000 description 5
- 230000007935 neutral effect Effects 0.000 description 5
- 238000005498 polishing Methods 0.000 description 5
- 230000002485 urinary effect Effects 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000006555 catalytic reaction Methods 0.000 description 4
- 238000002791 soaking Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- 238000004627 transmission electron microscopy Methods 0.000 description 2
- -1 3,3,5, 5-tetramethylBiphenyl amine Chemical class 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 229910004664 Cerium(III) chloride Inorganic materials 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
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- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229910000420 cerium oxide Inorganic materials 0.000 description 1
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- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Inorganic materials O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 1
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- 230000004112 neuroprotection Effects 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- BMMGVYCKOGBVEV-UHFFFAOYSA-N oxo(oxoceriooxy)cerium Chemical compound [Ce]=O.O=[Ce]=O BMMGVYCKOGBVEV-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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Abstract
本发明公开一种基于CeO2纳米棒的尿糖试纸及其制备方法，首先将铈盐和无机碱混合后充分搅拌，形成白色悬浮液后移入高压反应釜中，在烘箱中进行水热反应；弃上清，将沉淀物洗涤至中性，干燥后得到CeO2纳米棒；将CeO2纳米棒与显色剂、葡萄糖氧化酶按比例混合制得溶液，将空白试纸浸入溶液中，取出试纸后烘干，即得基于CeO2纳米棒的尿糖试纸。将该试纸置于含有不同浓度葡萄糖的环境中后取出会显色，在低浓度阶段颜色较浅，随着葡萄糖浓度的加深，试纸呈现的颜色也逐步梯度加深，各阶段区别明显，检测灵敏度高；本申请打破了CeO2纳米材料的应用领域局限，有助于进一步拓宽其市场应用范围。
The invention discloses a urine sugar test paper based on CeO2 nanorods and a preparation method thereof. First, a cerium salt and an inorganic base are mixed and fully stirred to form a white suspension, which is then moved into a high-pressure reactor, and subjected to a hydrothermal reaction in an oven; Discard the supernatant, wash the precipitate to neutrality, and dry to obtain CeO 2 nanorods; mix the CeO 2 nanorods with the developer and glucose oxidase in proportion to prepare a solution, immerse the blank test paper in the solution, and take out the test paper. After drying, the urine sugar test paper based on CeO 2 nanorods is obtained. When the test paper is placed in an environment containing different concentrations of glucose, the color will develop. In the low concentration stage, the color is lighter. With the deepening of the glucose concentration, the color of the test paper gradually deepens. The difference between each stage is obvious and the detection sensitivity is high. ; This application breaks the limitation of the application field of CeO 2 nanomaterials and helps to further broaden its market application range.
Description
Technical Field
The invention belongs to the technical field of medicinal detection, and particularly relates to a CeO-based reagent2A method for preparing a nanorod test paper for glucose in urine.
Background
Nanoenzymes (also known as nanomimic enzymes) are an extremely important branch of the field of artificial enzymes. In recent decades, researchers have provided different natural enzyme activities by controlling the size and surface functional groups of nanomaterials. Compared with natural enzymes and other mimic enzymes, the nano-enzyme has other properties peculiar to nano-materials such as optics, electrics, magnetics and the like besides catalytic activity, so that the nano-enzyme has double or even multiple functions. In addition, the large specific surface area and abundant surface chemical properties of the nano material enable the nano enzyme to be easy to chemically modify and connect various biomolecules, so that biological probes and sensors can be conveniently constructed. Nano-enzyme based on nano-materials has been widely used in the fields of biosensors, immunoassays, cancer diagnosis and treatment, neuroprotection, contaminant removal, and the like.
CeO2Is a common catalytic material. CeO at the nanoscale due to the presence of surface oxygen vacancies2Ce in (1)3+And Ce4+Being able to reversibly convert to produce a catalytic effect, it is one of the nanoenzymes of great interest. A large number of studies have demonstrated that CeO2Can effectively simulate superoxide dismutase, catalase, peroxidase, oxidase, phosphatase, etc. As a novel nano enzyme, the nano cerium dioxide has high chemical stability and catalytic stability and has wide application prospect under various conditions.
Currently CeO2Mainly focused on catalysis, and in recent years, CeO2The nanometer material plays more and more important roles in the field of catalysis, is applied to catalytic reactions such as CO oxidation, NOx reduction, methanol oxidation and the like, and Chinese patent CN 112516997A discloses CeO2/MnO2The preparation method of the nano-rod has simple process, cerium salt and inorganic base are taken as main raw materials, the obtained nano-rod material has better stability and catalytic performance, and the sewage treatment surface is shownShowing remarkable effect. In chemical mechanical polishing, CeO2The polishing slurry is different from the traditional polishing slurry, can adsorb anions and cations simultaneously under the alkaline polishing condition, is amphoteric, and has better polishing performance. CeO (CeO)2The ultraviolet light absorbing agent has strong absorbing capacity within the range of 300-450 nm, particularly strong absorbing capacity to ultraviolet light, almost zero absorbing capacity to visible light and good light transmittance, can be used as an ultraviolet absorbent, and is applied to the aspects of ultraviolet-proof paint, sunscreen cosmetics, plastics and aging resistance and the like. The material can also be applied to fuel cells, wherein Solid Oxide Fuel Cells (SOFCs) are more researched, and the fuel cells have the advantages of high efficiency, no pollution, long service life, low production cost and the like, and are one of clean fuels. In addition, CeO is used as the cross and infiltration of nanotechnology and modern medicine and biology2Base nanostructure materials are gaining increasing attention in antioxidant biomedicine.
As can be seen from the above, CeO2The nano material is widely applied in many fields, but no technical personnel applying the nano material to test paper research and development are found so far, and if a test paper for detection with low cost, simple flow, easy industrial production and obvious color comparison is researched based on the nano material, the nano material can bring beneficial influence on further widening the market application range.
Disclosure of Invention
The invention aims to provide a CeO-based material2The nanorod test paper for urine glucose and the preparation method thereof have the advantages of simple preparation process, high color development sensitivity and popularization and application value, and can be used for detection in the fields of urine glucose, blood glucose and the like.
The technical scheme of the invention is as follows: based on CeO2The method for preparing the urine glucose test paper by the nano rods specifically comprises the following steps:
(1) mixing cerium salt and inorganic base, and fully stirring to form white suspension;
(2) transferring the white suspension into a high-pressure reaction kettle to perform hydrothermal reaction in an oven;
(3) discarding the supernatant, washing the precipitate to neutrality, and drying to obtainCeO2A nanorod;
(4) the CeO prepared in the step (3) is added2Mixing the nano-rods with a color developing agent and glucose oxidase in proportion to prepare a solution, immersing a blank test paper into the solution, taking out the test paper and drying to obtain the CeO-based test paper2And (3) a urinary sugar test paper of the nano-rod.
Further, in the step (1), the mass ratio of the cerium salt to the inorganic base is 1: 10-1: 20.
Further, in the step (2), the temperature for hydrothermal reaction in the oven is 90-140 ℃, and the reaction time is 7-15 h.
Further, in the step (3), CeO was obtained2The particle size of the nano rod is 70-260 nm.
Further, in the step (4), CeO2The mass ratio of the nanorods to the color developing agent to the glucose oxidase is 1:0.1: 0.1-1: 1: 1.
Further, the cerium salt used in step (1) is selected from the group consisting of cerium nitrate, cerium chloride and cerium sulfate; the range of choice of inorganic base used includes sodium hydroxide, aqueous ammonia and potassium hydroxide.
Further, the selection range of the color developer used in the step (4) includes 2, 2-diaza-bis-3-ethylbenzthiazoline-6-sulfonic acid, o-phenylenediamine and 3,3,5, 5-tetramethylbenzidine.
The test paper prepared by the method is soaked in glucose solutions with different concentrations for 3-5min and then taken out, the test paper shows other colors different from the background color of the test paper, and the color of the test paper tends to deepen in a gradient manner along with the increase of the concentration of glucose.
The basic principle of the preparation of the urine glucose test paper is as follows: since glucose oxidase can react with glucose (main component of urine sugar) to generate hydrogen peroxide and acid (lowering the pH of the system helps the reaction); the hydrogen peroxide reacts with the cerium oxide to generate hydroxyl radicals, which reoxidize color developers (TMB, OPD, etc.) to cause a color development reaction.
Compared with the prior art, the invention has the following advantages:
1. the application provides a CeO-based alloy2Simple and rapid qualitative detection of urine glucose and blood sugar concentration of nano-rodThe test paper is placed in an environment containing glucose with different concentrations and then taken out to be developed, the color is lighter in a low concentration stage, the color presented by the test paper is gradually deepened in a gradient manner along with the increase of the concentration of the glucose, the difference of each stage is obvious, and the detection sensitivity is high;
2. the test paper preparation method disclosed by the application mainly adopts simple inorganic materials as raw materials, is low in price, simple in overall preparation steps and short in preparation period, and is beneficial to large-scale popularization and application;
3. compared with CeO2The application of the nanometer material in the fields of common catalysis, ultraviolet absorption, fuel cells and the like explores a method based on CeO2The new application path of the nano material breaks through the limitation of the conventional field and is beneficial to further widening the market application range of the nano material.
Drawings
FIG. 1 shows CeO prepared in example one2Transmission electron microscopy spectra of the nanorods;
FIG. 2 shows CeO prepared in example II2XRD pattern of the nanorods;
FIG. 3 is a CeO-based alloy prepared in example III2The color development effect of the nanorod test paper for glucose is shown after detecting glucose with different concentrations.
Detailed Description
The technical solution of the present invention is further described below with reference to the accompanying drawings, but not limited thereto, and any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention shall be covered by the protection scope of the present invention.
The first embodiment is as follows:
weighing 1g Ce (NO)3)3·6H2Mixing O and 12g NaOH, stirring for 1h at room temperature to obtain white suspension, transferring the white suspension into a high-pressure reaction kettle, performing hydrothermal reaction for 9h in a drying oven at 110 ℃, pouring out supernatant, washing precipitate to be neutral, and drying a sample to constant weight by using a freeze dryer (FD-1D-80, Beijing Bo Yi kang laboratory instrument) at room temperature to obtain CeO2A nanorod sample; adding CeO2Nanorods with 3,3,5, 5-tetramethylBiphenyl amine (TMB) and glucose oxidase are mixed according to the mass ratio of 1:1:1, mixing and stirring to prepare a solution, soaking the solution in blank test paper (qualitative filter paper, Hangzhou special paper industry Co., Ltd.) for a certain time, taking out the test paper and drying to obtain the CeO-based test paper2A novel urine glucose test paper of a nano rod.
CeO obtained in example2The nanorod samples were microscopically morphologically analyzed by transmission electron microscopy. As a result, as shown in FIG. 1, it was observed that the obtained sample had a uniform rod-like structure and had a particle diameter of 70 to 260 nm.
Example two:
weighing 1g Ce (NO)3)3·6H2Mixing O and 12g NaOH, stirring for 1h at room temperature to obtain white suspension, transferring the white suspension into a high-pressure reaction kettle, carrying out hydrothermal reaction for 9h in an oven at 130 ℃, pouring out supernatant, and washing a precipitate to be neutral. Then drying the sample to constant weight by using a freeze dryer (FD-1D-80, Beijing Bo Yi kang laboratory instrument) at room temperature to obtain CeO2Nanorod sample, CeO2The mass ratio of the nano rod to TMB and glucose oxidase is 1: 0.5: 1, mixing and stirring to prepare a solution, putting the solution into a blank test paper, soaking for a certain time, taking out the test paper and drying to obtain the CeO-based test paper2A novel urine glucose test paper of a nano rod.
Microscopic morphological analysis is carried out on the sample through a transmission electron microscope, and the obtained sample presents a uniform rod-shaped structure with the grain diameter of 80-240 nm.
CeO obtained in example2Urine glucose test paper, X-ray diffractometer (XRD) on CeO2And (5) carrying out phase structure analysis. As a result, as shown in FIG. 2, it was found that clear CeO was observed2Characteristic peak.
CeO obtained in this example2The test paper for urine glucose was immersed in glucose solutions of 0mmol/L, 5mmol/L, 15mmol/L, 25mmol/L, 35mmol/L and 55mmol/L for five minutes and then taken out with clean tweezers, and the results are shown in FIG. 3, where the test paper immersed in glucose solution of 5mmol/L and taken out to showDifferent from the very light blue color of the test paper of the white control group, the color of the test paper is sequentially deepened along with the increase of the glucose concentration, and the color change has obvious gradient relation, so that the human eye recognition degree is high, and the test paper can be used for detecting urine glucose.
Example three:
weighing 1.2g Ce (NO)3)3·6H2Mixing O and 12g KOH, stirring for 1h at room temperature to obtain white suspension, transferring the white suspension into a high-pressure reaction kettle, carrying out hydrothermal reaction for 9h in an oven at 130 ℃, pouring out supernatant, and washing a precipitate to be neutral. Then drying the sample to constant weight by using a freeze dryer (FD-1D-80, Beijing Bo Yi kang laboratory instrument) at room temperature to obtain CeO2A nanorod sample. Adding CeO2The nano-rods, 2-dinitro-bis-3-ethylbenzthiazoline-6-sulfonic Acid (ABTS) and glucose oxidase are mixed according to the mass ratio of 1: 0.5: 1, mixing and stirring the solvent to prepare a solution, putting the solution into a test paper, soaking the test paper for a certain time, taking out the test paper and drying the test paper to obtain the CeO-based reagent2And (3) a urinary sugar test paper of the nano-rod.
Microscopic morphological analysis is carried out on the sample through a transmission electron microscope, and the obtained sample presents a uniform rod-shaped structure with the grain diameter of 90-260 nm.
CeO obtained in this example2The urine glucose test paper is used for carrying out a glucose concentration test experiment, the test paper is respectively immersed in glucose solutions with the concentrations of 0mmol/L, 5mmol/L, 15mmol/L, 25mmol/L, 35mmol/L and 55mmol/L, after immersion for five minutes, the test paper is taken out by using a clean forceps, after immersion in the glucose solution with the concentrations of 5mmol/L, the test paper can be taken out to show a very light green color different from that of a white control group, along with the increase of the glucose concentration, the color of the test paper is sequentially deepened, and obvious gradient relation exists in the color change, so that the human eye recognition degree is high, and the test paper can be used for detecting urine glucose.
Example four:
weighing 1g of CeCl3·6H2O and 12g NH3·H2Mixing with O, stirring at room temperature for 1 hr to obtain white suspension, transferring the white suspension into a high-pressure reaction kettle, and heating in an oven at 130 deg.C to obtain waterThe reaction was heated for 9h, the supernatant decanted, and the precipitate washed to neutral. Then drying the sample to constant weight by using a freeze dryer (FD-1D-80, Beijing Bo Yi kang laboratory instrument) at room temperature to obtain CeO2Nanorod sample, CeO2The nano-rod, o-phenylenediamine (OPD) and glucose oxidase are mixed according to the mass ratio of 1: 0.5: 1, mixing and stirring to prepare a solution, putting the solution into a blank test paper, soaking for a certain time, taking out the test paper and drying to obtain the CeO-based test paper2And (3) a urinary sugar test paper of the nano-rod.
CeO obtained in this example2The urine glucose test paper is used for carrying out a glucose concentration test experiment, the test paper is respectively immersed in glucose solutions with the concentrations of 0mmol/L, 5mmol/L, 15mmol/L, 25mmol/L, 35mmol/L and 55mmol/L, the test paper is taken out by using clean tweezers after being immersed for five minutes, the test paper is taken out after being immersed in the glucose solution with the concentration of 5mmol/L and can show very light yellow different from that of a white control group, the color of the test paper is sequentially deepened along with the increase of the glucose concentration, an obvious gradient relation exists in the color change, the human eye recognition degree is high, and the test paper can be used for detecting urine glucose.
Example five:
weighing 1g Ce (NO)3)3·6H2Mixing O and 12g KOH, stirring for 1h at room temperature to obtain white suspension, transferring the white suspension into a high-pressure reaction kettle, carrying out hydrothermal reaction for 9h in an oven at 130 ℃, pouring out supernatant, and washing a precipitate to be neutral. Then drying the sample to constant weight by using a freeze dryer (FD-1D-80, Beijing Bo Yi kang laboratory instrument) at room temperature to obtain CeO2Nanorod sample, CeO2The mass ratio of the nano rod to TMB and glucose oxidase is 1: 0.5: 0.5 is mixed and stirred to prepare a solution, the solution is put into test paper to be soaked for a certain time, the test paper is taken out and dried, and the CeO-based test paper is obtained2And (3) a urinary sugar test paper of the nano-rod.
Example six:
weighing 1g Ce (NO)3)3·6H2Mixing O and 12g NaOH, stirring at room temperature for 1h to obtain white suspension, transferring the white suspension into a high-pressure reaction kettle, performing hydrothermal reaction in an oven at 120 ℃ for 10h, pouring out supernatant, and settlingThe precipitate is washed to neutrality. Then drying the sample to constant weight by using a freeze dryer (FD-1D-80, Beijing Bo Yi kang laboratory instrument) at room temperature to obtain CeO2Nanorod sample, CeO2The mass ratio of the nano rod to ABTS and glucose oxidase is 1: 0.5: 0.7 is mixed and stirred to prepare a solution, the solution is put into test paper to be soaked for a certain time, the test paper is taken out and dried, and the CeO-based test paper is obtained2And (3) a urinary sugar test paper of the nano-rod.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (8)
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| CN114354718A (en) * | 2022-03-18 | 2022-04-15 | 南京邮电大学 | Application of Nanozymes in Catalytic Enhancement of Electrochemiluminescence Signals and Imaging of Single Cell Surface Membrane Proteins |
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| CN112516997A (en) * | 2020-11-30 | 2021-03-19 | 南京邮电大学 | Preparation method of CeO2/MnO2 nanorods |
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