Cell storage device and cell storage method for optimizing cell preservation
Technical Field
The invention relates to the technical field of cell storage, in particular to a cell storage device and a cell storage method for optimizing cell preservation.
Background
At present, stem cell technology has become a breakthrough for overcoming various difficult and complicated diseases in human in this century, stem cell transplantation for treating many difficult and complicated diseases such as heart diseases, nervous system diseases, blood diseases and the like has become a hotspot of stem cell research and application at present, and also serves as a method for clinically treating the difficult and complicated diseases. Stem cell research and clinical application are a systematic work which is coordinated and completed by multiple subjects, multiple units and multiple departments, the culture technology of stem cells is mature day by day, and the application of stem cells is also deeply developed; in the clinical research and treatment of stem cells, an independent cell preparation unit is required to provide cells, and the cells are required to be provided to a cell use unit, so that the cells can achieve the optimal activity no matter research and development or clinical application, and a cell storage and transportation process which can reach the standard specification is required.
Therefore, it is the key of the present invention to provide a stem cell environment suitable for the survival activity of cells, and to facilitate the storage and transportation of stem cells, and to ensure the safety during the storage and transportation.
Disclosure of Invention
In order to overcome the above technical problems, the present invention provides a cell storage device and a cell storage method for optimizing cell preservation, comprising: (1) the first connecting pipe is connected with the water tank, the second connecting pipe is connected with the disinfectant tank, the infusion pump is started, the valve on the first connecting pipe is opened to convey clean water to the adapter pipe, the clean water is conveyed to the branch pipe through the infusion pipe and is injected into the cell storage box, the inner cavity of the cell storage box is cleaned, the cleaning water is discharged, the valve on the first connecting pipe is closed, the valve on the second connecting pipe is opened, the disinfectant is conveyed to the sprayer through the infusion pipe, the sprayer atomizes the disinfectant and fills the cell storage box for disinfection, the ultraviolet sterilizing lamp is opened for sterilization at the same time, the inner cavity of the cell storage box is dried, the sealing cover is opened, the storage seat is pulled by a handle to enable the storage seat to slide on the sliding rail through the sliding block until the storage seat is moved out of the inner cavity of the cell storage box, cell products are placed in the glass container, and then the glass container is placed in the placing groove after sealing, the method comprises the steps of injecting clear water into a refrigerating chamber, restoring a storage seat to the original position after all glass containers are placed, closing a sealing cover, starting a refrigerating machine to operate to cool the clear water in the refrigerating chamber, further cooling the glass containers, holding a push rod by hand, pushing a cell storage device to move, moving the cell storage device to a storage area, and storing the cell storage device, and solves the problems that the existing stem cells cannot keep a good cell activity for a long time and cannot be stored at a proper temperature for a long time; (2) this cell bin is through first damper, second damper carries out the damping, carry out the damping through the first damping spring of a plurality of, the vibrations of having guaranteed installation base department are effectively eliminated, avoid transmitting to the installation cover, carry out the damping through a plurality of second damping spring, it removes in damping mount to drive the profile of tooth post simultaneously, thereby it rotates to drive the connecting rod through the gear, thereby realize the extension and the shrink of telescopic link, further eliminate vibrations, it is difficult to the transportation to have solved current stem cell, be difficult to guarantee the stability of haulage equipment even in the transportation, easily cause the cracked problem of stem cell transportation blood bag.
The purpose of the invention can be realized by the following technical scheme:
a cell storage device for optimizing cell preservation comprises an installation base, an installation cover, a cell storage box, a sealing cover, a push rod, an infusion pump, a switching tube, a liquid inlet tube and an infusion tube, wherein universal wheels are installed at the corners of the bottom of the installation base, the installation cover is sleeved at the top of the installation base, the cell storage box is installed at one end of the top of the installation cover, the infusion pump is installed at the other end of the top of the installation cover, the liquid inlet tube is installed at the input end of the infusion pump and communicated to the top of an inner cavity of the switching tube, a first connecting tube and a second connecting tube are installed at two ends of one side of the switching tube respectively, valves are installed on the first connecting tube and the second connecting tube, the switching tube is installed at the top of the installation cover, the infusion tube is installed at the output end of the infusion pump, and the infusion tube penetrates through one side of the cell storage box, the top hinged joint of cell bin has sealed lid, the push rod is installed to the top of installation cover one end that is close to the cell bin.
As a further scheme of the invention: the utility model discloses a cell storage box, including sealed lid, cell bin, handle, storage seat, ultraviolet germicidal lamp, the interior border position of sealed lid all installs the sterilamp, the inner chamber of cell bin is provided with stores the seat, the handle is all installed to the top both sides of storing the seat, a plurality of standing groove has been seted up on the storage seat, the inside of storing the seat is provided with the refrigeration room, a plurality of the standing groove all is located the refrigeration room, install the refrigerator in the bottom inner wall of storage seat.
As a further scheme of the invention: the cell storage box is characterized in that slide rails are arranged on the inner walls of four sides of the cell storage box, slide blocks are connected to the slide rails in a sliding mode, the slide blocks are arranged on the outer walls of the four sides of the storage seat respectively, a lifting mechanism is arranged at the bottom of the storage seat and is arranged on a mounting box, and the mounting box is arranged at the bottom of an inner cavity of the cell storage box.
As a further scheme of the invention: elevating system includes jacking board, crane, the bottom of storing the seat is connected to the jacking board, the crane is all installed to the bottom both sides of jacking board, two the bottom of crane is installed respectively on two boards of placing, two place the board and install respectively on the both sides inner wall of mounting box, the atomizer is installed to the inner chamber bottom of mounting box, one side of atomizer is connected to the one end of transfer line, the branch pipe is installed to the bottom of transfer line, all install the solenoid valve on transfer line and the branch pipe.
As a further scheme of the invention: install first damper, second damper, four between installation base, the installation cover second damper installs at four corners at installation base top, a plurality of first damper equidistance distributes at the top of installation base.
As a further scheme of the invention: first damper includes spliced pole, adapter sleeve, first damping spring, the bottom sliding connection of spliced pole is in the inner chamber of adapter sleeve, first damping spring is all installed to the top, the bottom of spliced pole, the inner chamber bottom at the installation cover is connected to the first damping spring at spliced pole top, the inner chamber bottom at the adapter sleeve is connected to the first damping spring of spliced pole bottom, the top at the installation base is installed to the adapter sleeve.
As a further scheme of the invention: the second damping mechanism comprises a damping base, a tooth-shaped column, a supporting frame, a gear, a connecting rod, a telescopic rod, a return spring, a mounting plate and a second damping spring, the top of the damping base is movably provided with a tooth-shaped column in a penetrating way, two sides of the tooth-shaped column are respectively connected with gears in a meshing way, the two gears are respectively and rotatably arranged on the tops of the two supporting frames, a connecting rod is arranged on the gear, one end of the connecting rod is rotatably connected to the top end of the telescopic rod, the telescopic rod is sleeved with a return spring, the top of the tooth-shaped column is provided with an installation plate, the top of the installation plate is provided with a plurality of second damping springs, the top ends of the second damping springs are connected to the bottom of the inner cavity of the installation cover, the bottom fixed mounting of vibration damping mount, support frame is at the top of installation base, the bottom of telescopic link is rotated and is installed at the top of installation base.
As a further scheme of the invention: a cell storage method for optimizing cell preservation comprising the steps of:
the method comprises the following steps: connecting a first connecting pipe with a water tank, connecting a second connecting pipe with a disinfectant tank, starting an infusion pump, opening a valve on the first connecting pipe to convey clean water into an adapter pipe, conveying the clean water to a branch pipe through an infusion pipe, injecting the branch pipe into a cell storage box, cleaning the inner cavity of the cell storage box, discharging the clean water, closing the valve on the first connecting pipe, opening the valve on the second connecting pipe, conveying disinfectant to a sprayer through the infusion pipe, atomizing the disinfectant by the sprayer, filling the cell storage box with the atomized disinfectant for disinfection, and simultaneously opening an ultraviolet germicidal lamp for disinfection;
step two: after the inner cavity of the cell storage box is dried, the sealing cover is opened, the storage seat is pulled by the handle, so that the storage seat slides on the sliding rail through the sliding block until the storage seat moves out of the inner cavity of the cell storage box;
step three: placing the cell product in a glass container, then placing the glass container in a placing groove after sealing, injecting clear water into the refrigerating chamber, restoring the storage seat to the original position after all the glass containers are placed, closing the sealing cover, starting a refrigerating machine to operate to cool the clear water in the refrigerating chamber, and further cooling the glass container;
step four: and the pushing rod is held by hand to push the cell storage device to move, and the cell storage device is moved to the storage area to be stored.
The invention has the beneficial effects that:
the invention relates to a cell storage device and a cell storage method for optimizing cell preservation, wherein a first connecting pipe is connected with a water tank, a second connecting pipe is connected with a disinfectant tank, a liquid conveying pump is started, a valve on the first connecting pipe is opened to convey clear water into an adapter pipe, the clear water is conveyed to a branch pipe through a liquid conveying pipe and is injected into the cell storage tank, the inner cavity of the cell storage tank is cleaned, the cleaning water is discharged, the valve on the first connecting pipe is closed, a valve on the second connecting pipe is opened, disinfectant is conveyed to a sprayer through the liquid conveying pipe, the sprayer atomizes the disinfectant and then fills the cell storage tank for disinfection, an ultraviolet sterilization lamp is simultaneously opened for sterilization, after the inner cavity of the cell storage tank is dried, a sealing cover is opened, a storage seat is pulled through a pull handle, the storage seat slides on a sliding rail through a sliding block until the storage seat is, then placing the glass containers in the placing groove after sealing, injecting clear water into the refrigerating chamber, restoring the storage seat to the original position after all the glass containers are placed, closing the sealing cover, starting the refrigerating machine to operate to cool the clear water in the refrigerating chamber, further cooling the glass containers, holding the push rod by hand, pushing the cell storage device to move, and moving the cell storage device to a storage area for storage; the cell storage device is characterized in that the cell storage box is cleaned by inputting clean water, the atomized disinfectant sprayed by the sprayer is used for disinfecting the cell storage box, the inner cavity of the cell storage box is sterilized by the ultraviolet germicidal lamp, so that an aseptic and sanitary environment is provided for cell preservation, the clean water is refrigerated by the refrigerator, so that the cells are indirectly refrigerated, a low-temperature environment is provided for the cells, a proper temperature environment is provided for cell survival, and the cell storage device is good in cell storage effect;
the cell storage device is subjected to vibration reduction through the first damping mechanism and the second damping mechanism, and is subjected to vibration reduction through the plurality of first damping springs, so that the vibration at the position of the mounting base is effectively eliminated, the vibration is prevented from being transmitted to the mounting cover, the vibration is reduced through the plurality of second damping springs, and meanwhile, the tooth-shaped column is driven to move in the damping base, so that the connecting rod is driven to rotate through the gear, the stretching and the contraction of the telescopic rod are realized, and the vibration is further eliminated; this cell storage device carries out the damping through first damper, second damper to guaranteed the stability of installation cover, then through turning into the glass container with cell goods, guaranteed the stability of glass container, thereby avoided the damage of cell.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic diagram of a cell storage device for optimized cell preservation according to the present invention;
FIG. 2 is a connection view of the adapter tube, the liquid inlet tube, the first connection tube and the second connection tube in the present invention;
FIG. 3 is a top view of the mounting cover and cell storage case of the present invention;
FIG. 4 is a schematic view showing the internal structure of the cell storage case of the present invention;
FIG. 5 is a schematic view of the internal structure of the storage holder according to the present invention;
FIG. 6 is a schematic view of the construction of the lifting mechanism of the present invention;
FIG. 7 is a schematic view showing the internal structure of the mounting case of the present invention;
FIG. 8 is a view showing the connection of the infusion tube and the branch tube in the present invention;
FIG. 9 is a schematic view showing the internal structure of the mounting base and the mounting cover according to the present invention;
FIG. 10 is a schematic view of the structure of a first damper mechanism according to the present invention;
fig. 11 is a schematic structural view of the second damper mechanism in the present invention.
In the figure: 101. installing a base; 102. mounting a cover; 103. a cell storage bin; 104. a sealing cover; 105. a push rod; 106. an infusion pump; 107. a transfer tube; 108. a liquid inlet pipe; 109. a transfusion tube; 110. a first connecting pipe; 111. a second connecting pipe; 112. an ultraviolet germicidal lamp; 113. a storage seat; 114. a placement groove; 115. a handle; 116. mounting a box; 117. a lifting mechanism; 118. a slider; 119. a slide rail; 120. a refrigeration compartment; 121. a refrigerator; 122. a jacking plate; 123. a lifting frame; 124. placing the plate; 125. a sprayer; 126. a first damper mechanism; 127. a second damper mechanism; 128. connecting columns; 129. connecting sleeves; 130. a first damping spring; 131. a damping mount; 132. a toothed column; 133. a support frame; 134. a gear; 135. a connecting rod; 136. a telescopic rod; 137. a return spring; 138. mounting a plate; 139. a second damping spring; 140. and (4) branch pipes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
referring to fig. 1 to 11, the present embodiment is a cell storage device for optimizing cell preservation, including a mounting base 101, a mounting cover 102, a cell storage box 103, a sealing cover 104, a push rod 105, an infusion pump 106, an adapter tube 107, a liquid inlet tube 108, and an infusion tube 109, universal wheels are mounted at corners of the bottom of the mounting base 101, the mounting cover 102 is sleeved on the top of the mounting base 101, the cell storage box 103 is mounted at one end of the top of the mounting cover 102, the infusion pump 106 is mounted at the other end of the top of the mounting cover 102, the liquid inlet tube 108 is mounted at an input end of the infusion pump 106, the liquid inlet tube 108 is communicated with the top of an inner cavity of the adapter tube 107, a first connecting tube 110 and a second connecting tube 111 are respectively mounted at two ends of one side of the adapter tube 107, valves are mounted on the first connecting tube 110 and the second connecting tube 111, and the adapter tube 107 is mounted at the top of the mounting cover 102, an infusion tube 109 is installed at the output end of the infusion pump 106, the infusion tube 109 penetrates through one side of the cell storage box 103, a sealing cover 104 is hinged to the top of the cell storage box 103, and a push rod 105 is installed at one end, close to the cell storage box 103, of the top of the installation cover 102;
the ultraviolet germicidal lamps 112 are mounted at the edge positions of the inner side of the sealing cover 104, the storage seat 113 is arranged in the inner cavity of the cell storage box 103, the handles 115 are mounted on two sides of the top of the storage seat 113, the storage seat 113 is provided with a plurality of placing grooves 114, the storage seat 113 is internally provided with a refrigeration chamber 120, the placing grooves 114 are all located in the refrigeration chamber 120, and the inner wall of the bottom of the storage seat 113 is provided with a refrigerator 121;
the four inner walls of the cell storage box 103 are all provided with slide rails 119, the slide rails 119 are connected with slide blocks 118 in a sliding manner, a plurality of slide blocks 118 are respectively arranged on the four outer walls of the storage seat 113, the bottom of the storage seat 113 is provided with a lifting mechanism 117, the lifting mechanism 117 is arranged on an installation box 116, and the installation box 116 is arranged at the bottom of the inner cavity of the cell storage box 103;
the lifting mechanism 117 comprises a lifting plate 122 and lifting frames 123, the lifting plate 122 is connected with the bottom of the storage seat 113, the lifting frames 123 are respectively installed on two sides of the bottom of the lifting plate 122, the bottom ends of the two lifting frames 123 are respectively installed on two placing plates 124, the two placing plates 124 are respectively installed on the inner walls of two sides of the installation box 116, a sprayer 125 is installed at the bottom of an inner cavity of the installation box 116, one side of the sprayer 125 is connected to one end of the infusion tube 109, a branch tube 140 is installed at the bottom of the infusion tube 109, and electromagnetic valves are installed on the infusion tube 109 and the branch tube 140;
a first damping mechanism 126 and a second damping mechanism 127 are arranged between the mounting base 101 and the mounting cover 102, four second damping mechanisms 127 are arranged at four corners of the top of the mounting base 101, and a plurality of first damping mechanisms 126 are equidistantly distributed on the top of the mounting base 101;
the first damping mechanism 126 comprises a connecting column 128, a connecting sleeve 129 and a first damping spring 130, the bottom end of the connecting column 128 is slidably connected in the inner cavity of the connecting sleeve 129, the first damping spring 130 is mounted at the top and the bottom of the connecting column 128, the first damping spring 130 at the top of the connecting column 128 is connected at the bottom of the inner cavity of the mounting cover 102, the first damping spring 130 at the bottom of the connecting column 128 is connected at the bottom of the inner cavity of the connecting sleeve 129, and the connecting sleeve 129 is mounted at the top of the mounting base 101;
the second damping mechanism 127 comprises a damping base 131, tooth-shaped columns 132, supporting frames 133, gears 134, connecting rods 135, telescopic rods 136, return springs 137, a mounting plate 138 and second damping springs 139, the tooth-shaped columns 132 are movably installed at the top of the damping base 131 in a penetrating mode, the gears 134 are connected to two sides of the tooth-shaped columns 132 in a meshed mode, the two gears 134 are respectively installed at the tops of the two supporting frames 133 in a rotating mode, the connecting rods 135 are installed on the gears 134, one ends of the connecting rods 135 are rotatably connected to the top ends of the telescopic rods 136, the return springs 137 are sleeved on the telescopic rods 136, the mounting plate 138 is installed at the top of the tooth-shaped columns 132, the second damping springs 139 are installed at the top of the mounting plate 138, the top ends of the second damping springs 139 are connected to the bottom of an inner cavity of the mounting cover 102, and the bottoms of the damping bases 131 and the supporting frames 133 are fixedly installed at the top of the mounting base 101, the bottom end of the telescopic rod 136 is rotatably installed on the top of the installation base 101.
Referring to fig. 1-11, the operation of a cell storage device for optimizing cell preservation according to the present embodiment is as follows:
the method comprises the following steps: connecting a first connecting pipe 110 with a water tank, connecting a second connecting pipe 111 with a disinfectant tank, starting an infusion pump 106, opening a valve on the first connecting pipe 110 to convey clean water into an adapter pipe 107, conveying the clean water into a branch pipe 140 through an infusion pipe 109, injecting the clean water into a cell storage box 103, cleaning the inner cavity of the cell storage box 103, discharging cleaning water, closing the valve on the first connecting pipe 110, opening the valve on the second connecting pipe 111, conveying disinfectant to a sprayer 125 through the infusion pipe 109, atomizing the disinfectant by the sprayer 125, filling the cell storage box 103 with the atomized disinfectant for disinfection, and simultaneously opening an ultraviolet sterilizing lamp 112 for sterilization;
step two: after the inner cavity of the cell storage box 103 is dried, the sealing cover 104 is opened, the storage seat 113 is pulled by the handle 115, so that the storage seat 113 slides on the slide rail 119 through the slide block 118 until the storage seat 113 moves out of the inner cavity of the cell storage box 103;
step three: placing the cell product in a glass container, then placing the glass container in the placing groove 114 after sealing, injecting clear water into the refrigerating chamber 120, restoring the storage seat 113 to the original position after all the glass containers are placed, closing the sealing cover 104, starting the refrigerating machine 121 to operate to cool the clear water in the refrigerating chamber 120, and further cooling the glass containers;
step four: the push rod 105 is held by hand to push the cell storage device to move, and the cell storage device is moved to the storage area to be stored.
Referring to fig. 1-11, the cell storage device for optimizing cell preservation according to the present embodiment works as follows:
in the part disclosed by the invention, a first connecting pipe 110 is connected with a water tank, a second connecting pipe 111 is connected with a disinfectant tank, a liquid delivery pump 106 is started, a valve on the first connecting pipe 110 is opened to deliver clean water into an adapter pipe 107, the clean water is delivered to a branch pipe 140 through a liquid delivery pipe 109 and is injected into a cell storage tank 103 to clean the inner cavity of the cell storage tank 103, cleaning water is discharged, the valve on the first connecting pipe 110 is closed, the valve on the second connecting pipe 111 is opened to deliver disinfectant to a sprayer 125 through the liquid delivery pipe 109, the sprayer 125 atomizes the disinfectant and fills the cell storage tank 103 for disinfection, an ultraviolet sterilizing lamp 112 is opened for sterilization, after the inner cavity of the cell storage tank 103 is dried, a sealing cover 104 is opened, a storage seat 113 is pulled through a handle 115 to enable the storage seat 113 to slide on a sliding rail 119 through a sliding block 118 until the storage, placing the cell product in a glass container, then placing the glass container in a placing groove 114 after sealing, injecting clear water into the refrigerating chamber 120, restoring the storage seat 113 to the original position after all the glass containers are placed, closing the sealing cover 104, starting the refrigerating machine 121 to operate to cool the clear water in the refrigerating chamber 120, further cooling the glass containers, holding the push rod 105 by hand, pushing the cell storage device to move, and moving the cell storage device to a storage area for storage; the cell storage device is characterized in that the cell storage box 103 is cleaned by inputting clean water, the atomized disinfectant is sprayed out of the sprayer 125 to disinfect the cell storage box 103, the inner cavity of the cell storage box 103 is sterilized by the ultraviolet sterilizing lamp 112, a sterile and sanitary environment is provided for cell storage, the clean water is refrigerated by the refrigerator 121 to indirectly refrigerate the cells, a low-temperature environment is provided for the cells, a proper temperature environment is provided for cell survival, and the cell storage device is good in cell storage effect;
the cell storage device is subjected to vibration reduction through the first damping mechanism 126 and the second damping mechanism 127, and is subjected to vibration reduction through the plurality of first damping springs 130, so that the vibration at the position of the mounting base 101 is effectively eliminated, the vibration is prevented from being transmitted to the mounting cover 102, the vibration is reduced through the plurality of second damping springs 139, and meanwhile, the tooth-shaped column 132 is driven to move in the damping base 131, so that the connecting rod 135 is driven to rotate through the gear 134, the stretching and the contraction of the telescopic rod 136 are realized, and the vibration is further eliminated; the cell storage device is damped by the first damping mechanism 126 and the second damping mechanism 127, so that the stability of the mounting cover 102 is ensured, and then the cell product is transferred into the glass container, so that the stability of the glass container is ensured, and the damage of cells is avoided.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.