CN113207885B - Application of bentyprazole in promoting rice tiller differentiation and regenerating rice planting - Google Patents
Application of bentyprazole in promoting rice tiller differentiation and regenerating rice planting Download PDFInfo
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- CN113207885B CN113207885B CN202110406071.1A CN202110406071A CN113207885B CN 113207885 B CN113207885 B CN 113207885B CN 202110406071 A CN202110406071 A CN 202110406071A CN 113207885 B CN113207885 B CN 113207885B
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- 241000209094 Oryza Species 0.000 title claims abstract description 91
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 90
- 235000009566 rice Nutrition 0.000 title claims abstract description 90
- 230000004069 differentiation Effects 0.000 title claims abstract description 17
- 230000001737 promoting effect Effects 0.000 title claims abstract description 17
- 230000001172 regenerating effect Effects 0.000 title description 3
- 238000000034 method Methods 0.000 claims description 13
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- SLLFVLKNXABYGI-UHFFFAOYSA-N 1,2,3-benzoxadiazole Chemical compound C1=CC=C2ON=NC2=C1 SLLFVLKNXABYGI-UHFFFAOYSA-N 0.000 claims description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000009340 sequential cropping Methods 0.000 claims description 3
- YJZAJOVGLGFSIF-UHFFFAOYSA-N 2-phenylmethoxy-1h-pyrrole Chemical compound C=1C=CC=CC=1COC1=CC=CN1 YJZAJOVGLGFSIF-UHFFFAOYSA-N 0.000 claims description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 2
- 230000000895 acaricidal effect Effects 0.000 claims description 2
- 239000000642 acaricide Substances 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 claims description 2
- 239000003899 bactericide agent Substances 0.000 claims description 2
- 239000003337 fertilizer Substances 0.000 claims description 2
- 230000002363 herbicidal effect Effects 0.000 claims description 2
- 239000004009 herbicide Substances 0.000 claims description 2
- 239000002917 insecticide Substances 0.000 claims description 2
- NDNKHWUXXOFHTD-UHFFFAOYSA-N metizoline Chemical compound CC=1SC2=CC=CC=C2C=1CC1=NCCN1 NDNKHWUXXOFHTD-UHFFFAOYSA-N 0.000 claims description 2
- 229960002939 metizoline Drugs 0.000 claims description 2
- 239000005645 nematicide Substances 0.000 claims description 2
- 239000005648 plant growth regulator Substances 0.000 claims description 2
- 239000003516 soil conditioner Substances 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000008096 xylene Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 22
- 230000001965 increasing effect Effects 0.000 abstract description 11
- 230000012010 growth Effects 0.000 abstract description 7
- IHHMUBRVTJMLQO-UHFFFAOYSA-N Pyraclonil Chemical compound C#CCN(C)C1=C(C#N)C=NN1C1=NN(CCCC2)C2=C1Cl IHHMUBRVTJMLQO-UHFFFAOYSA-N 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 210000005069 ears Anatomy 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000005507 spraying Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 4
- WKBPZYKAUNRMKP-UHFFFAOYSA-N 1-[2-(2,4-dichlorophenyl)pentyl]1,2,4-triazole Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(CCC)CN1C=NC=N1 WKBPZYKAUNRMKP-UHFFFAOYSA-N 0.000 description 3
- BAXOFTOLAUCFNW-UHFFFAOYSA-N 1H-indazole Chemical compound C1=CC=C2C=NNC2=C1 BAXOFTOLAUCFNW-UHFFFAOYSA-N 0.000 description 3
- 244000287680 Garcinia dulcis Species 0.000 description 3
- 239000005813 Penconazole Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- OCAPBUJLXMYKEJ-UHFFFAOYSA-N 1-[biphenyl-4-yl(phenyl)methyl]imidazole Chemical compound C1=NC=CN1C(C=1C=CC(=CC=1)C=1C=CC=CC=1)C1=CC=CC=C1 OCAPBUJLXMYKEJ-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- ZOMSMJKLGFBRBS-UHFFFAOYSA-N bentazone Chemical compound C1=CC=C2NS(=O)(=O)N(C(C)C)C(=O)C2=C1 ZOMSMJKLGFBRBS-UHFFFAOYSA-N 0.000 description 2
- 229960002206 bifonazole Drugs 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- BCMCBBGGLRIHSE-UHFFFAOYSA-N 1,3-benzoxazole Chemical compound C1=CC=C2OC=NC2=C1 BCMCBBGGLRIHSE-UHFFFAOYSA-N 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Wood Science & Technology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Ecology (AREA)
- Botany (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses application of pyraclonil in promoting rice shoot differentiation and ratoon rice planting. The pyraclonil can be used for promoting the growth of ratoon rice and the tillering bud differentiation of rice, has a regulation and control effect on the growth of ratoon rice, has small environmental pollution, can be widely applied to the planting of ratoon rice of different rice varieties in different areas, and has obvious effects of increasing the ear and increasing the yield of ratoon rice planting.
Description
Technical Field
The invention belongs to the technical field of crop production, and particularly relates to application of benzoxadiazole in promotion of rice shoot bud differentiation and ratoon rice planting.
Background
The regenerated rice is a rice planting mode which utilizes rice stakes obtained after first-season rice is harvested, adopts certain management and cultivation measures, enables dormant tillers on the rice stakes to quickly germinate into seedlings, sprout ears and bear fruits, and harvests again. When the rice is mature, a plurality of tillering buds usually exist on a plurality of sections, after the first season is harvested, the tillering buds grow again and shoot, and the tillering buds grow and develop for about 2 months and are mature again to be harvested. The ratoon rice has the advantages of short growth period, seed saving, labor adjustment, cost saving, water saving, full utilization of light temperature resources, high benefit and the like. In order to improve the yield, the key point of the production of the regenerated rice is to promote the differentiation of tiller/axillary bud so as to improve the number of regenerated effective ears, however, the number of growth regulators which can be used for the production of the regenerated rice at the present stage is not large; some synthetic substances play an important role in regulating the growth and development, immunity and resistance of plants, but different substances regulate different plants at different levels. Therefore, finding new substances to regulate rice regeneration becomes a possible solution.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides the application of the benconazole in promoting the rice shoot bud differentiation, and the benconazole can be used for regulating and controlling the growth and development of plants.
The invention also provides application of the benconazole in promoting the planting of ratoon rice.
The invention also provides a planting method of the ratoon rice.
The invention also provides a method for promoting rice tillering bud differentiation.
According to a first aspect of the present invention, the use of bifonazole in ratoon rice planting is proposed.
According to a second aspect of the present invention, the use of benzoxadiazole for promoting the shoot differentiation of rice is provided.
According to a third aspect of the present invention, there is provided a method of promoting the planting of ratoon rice, comprising the steps of: the above-mentioned benazol is dissolved and sprayed on the rice.
In some embodiments of the present invention, the solvent for preparing the solution of benzoxyazole is at least one selected from the group consisting of absolute ethanol, distilled water, acetone, toluene, and xylene.
In some embodiments of the present invention, the concentration of the sprayed benzypazole solution is 10 to 50 μ M.
In some embodiments of the present invention, the concentration of the sprayed benzypazole solution is 15 to 50 μ M.
In some embodiments of the invention, the concentration of the sprayed benzypazole solution is 30 μ M.
In some embodiments of the invention, the amount of the sprayed benzpyrazole solution is 15-20 liters/acre.
In some embodiments of the invention, the method further comprises adding or not adding a bactericide, insecticide, acaricide, nematicide, herbicide, plant growth regulator, fertilizer or soil conditioner to the solution of benzoxap.
In some embodiments of the invention, the height of the rice stump is 10-50 cm.
According to a fourth aspect of the present invention, there is provided a method for promoting rice shoot differentiation, comprising the steps of: the solution of benzoprazole was sprayed on rice.
The application of the benconazole according to the embodiment of the invention in promoting the rice shoot bud differentiation and the ratoon rice planting has at least the following beneficial effects: the benconazole provided by the scheme of the invention can be used for promoting the differentiation and growth of tillers/axillary buds, so that the generation of effective spikes of the regenerated rice is improved, the benconazole has a regulation and control effect on the growth of the regenerated rice, has no pollution to the environment, can be widely applied to the planting of the regenerated rice of different rice varieties in different areas, and has obvious spike increasing and yield increasing effects on the planting of the regenerated rice.
Drawings
The invention is further described with reference to the following figures and examples, in which:
FIG. 1 is a graph showing the results of the effect of penconazole on the height of the Meixiang No. 2 ratoon rice stump in example 1 of the present invention, wherein the scale in the graph is 10 cm;
FIG. 2 is a graph showing the results of the effect of benzpyrazole on the height of the stake of Yuenongsimiao rata in example 1 of the present invention, wherein the scale in the graph is 10 cm;
FIG. 3 is a graph showing the effect of pyraclonil on the number of ears per plant and the number of seeds per ear at different stake heights of Yuenongsimiao rata in example 1 of the present invention;
FIG. 4 is a graph showing the effect of benzpyrazole on setting percentage and effective ear ratio of Yuenongsimiao rata of different height of the stake in the rice of Yuenongsimiao in example 1 of the present invention;
FIG. 5 is a graph showing the effect of penconazole on the number of ears per plant and the number of grains per ear per plant at different stake heights of Meixiang No. 2 ratoon rice in example 1 of the present invention;
FIG. 6 is a graph showing the effect of penconazole on the seed setting rate and the effective ear ratio of Meixiang No. 2 ratoon rice at different height according to the present invention in example 1.
Detailed Description
The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments to fully understand the objects, features and effects of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and those skilled in the art can obtain other embodiments without inventive effort based on the embodiments of the present invention, and all embodiments are within the protection scope of the present invention. The test methods used in the examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are commercially available reagents and materials unless otherwise specified.
Preparing a benazol solution: 0.0403g of the raw benazol is taken, dissolved in absolute ethyl alcohol and then fixed to 10ml by using distilled water to prepare 10mM benazol mother liquor. When in use, the mother liquor is respectively diluted into 10, 30 and 50 mu M of the benzoxap reagent.
Ratooning rice test: the regenerated rice spraying test was carried out by using 2 varieties of rice stakes of Meixiangzhan No. 2 and Yuenongsimiao obtained experimentally by the Hongshou agricultural science and technology cooperative society of Yiyang Datong lake.
Example 1 influence of Rice piling height on Rice seed setting percentage
2 rice ears of rice varieties of 'Meixiangzhan No. 2' and 'Yuenongsimiao' are selected, cut and planted in cement buckets filled with rice soil, and 2 rice ears in each bucket are planted in 100 buckets in total. After transplanting and seedling slowing, retaining piles (15cm, 30cm and 50cm) with different heights on the rice. The spray application was carried out with benazol at a concentration of 30 μ M. The control group was sprayed with equal amount of clear water. After the solution of the benazolin is sprayed, the rice with different treatment pile heights is photographed, and the photographing is carried out once every 7 days until the ratoon rice is mature.
The experimental results are shown in fig. 1-6, fig. 1 is a result graph of the effect of the benconazole on the heights of the meixiang No. 2 regenerated rice stumps, as can be seen from fig. 1, the benconazole solution has a significant effect of promoting the tillering buds of the regenerated rice stumps with different stump heights of the meixiang No. 2 compared with a control group, fig. 2 is a result graph of the effect of the benconazole on the heights of the regenerative rice stumps of the Guangdong silk seedlings, as can be seen from fig. 2, the benconazole has a significant effect of promoting the differentiation of the regenerative rice tillering buds of the Guangdong silk seedlings with different stump heights; fig. 3 is a graph showing the influence of the benconazole on the number of single ear and the number of single ear at different pile heights of the yuenong sprout regenerated rice, and it can be seen from fig. 3 that after the benconazole solution is treated, the number of single ear and the number of single ear at different pile heights are obviously increased compared with the control group, wherein the benconazole has the strongest effect on the height of 30cm pile; fig. 4 is a graph showing the effect of the benconazole on the setting percentage and the effective ear ratio of the yuenongsimiao regenerated rice at different post heights, and it can be seen from fig. 4 that the setting percentage and the effective ear ratio of the yuenongsimiao treated by the benconazole solution at different post heights are obviously increased compared with the control group; fig. 5 is a graph showing the effect of the bentazon on the number of ears per plant and the number of grains per ear at different pile heights of the Meixiang No. 2 ratoon rice, and it can be seen from fig. 5 that after the treatment of the bentazon solution, the number of ears per plant at different pile heights of the Meixiang No. 2 ratoon rice is obviously increased compared with the control group; fig. 6 is a graph showing the effect of the benconazole on the maturing rate and the effective ear ratio of meixiang No. 2 ratoon rice at different pile heights, and it can be seen from fig. 6 that the maturing rate and the effective ear ratio of meixiang No. 2 ratoon rice at different pile heights after the treatment of the benconazole solution are obviously increased compared with the control group.
Example 2 Effect of spray concentration of Benzoxazole on Rice shoot number
2 rice varieties of 'Meixiangzhan No. 2' and 'Yuenongsimiao' were selected, rice ears were cut off, 30cm rice stakes were planted in cement buckets filled with rice soil, 2 plants per bucket were planted in 30 buckets in total, and a solution of bifonazole (concentration of 10. mu.M, 30. mu.M, 50. mu.M) was sprayed without any treatment on the control group. After the solution of the benconazole is sprayed, the rice with different processing pile heights is photographed, the photographing is carried out once every 7 days until the ratoon rice is mature, and the average number of new tillering buds of each rice plant is recorded.
TABLE 1
| Concentration of benazol | Average newly increased sprout number of Meixiang No. 2 | Mean number of newly increased sprout of Yuenongsimiao |
| Control group | 5 | 6 |
| 10μM | 6 | 6 |
| |
10 | 8 |
| 50μM | 9 | 7 |
The experimental results are shown in table 1, table 1 shows the influence of spraying different concentrations of benconazole reagent in henxiang account for No. 2 and the average number of new tillering buds of yuenong sprout, and it can be seen from the table that benconazole has the effect of increasing tillering bud branches of two different types of rice, wherein the effect on mexiang account for No. two is more obvious, and the average number of new tillering buds of rice reaches the maximum under the condition that the concentration of benconazole is 30 μ M.
Example 3 Rice field test
On the basis of the laboratory test, after first season rice was harvested using 2 varieties of rice, Meixiangzhan No. 2 and Yuenongsimiao, in a test field of the Miao agriculture science and technology cooperative in the Yiyang great lake region. The mode of district partition is adopted, and the height of the pile is 30 cm. The benconazole reagent is sprayed according to the concentration of 30 mu M, the amount of the benconazole solution sprayed is 15-20 liters/mu, and the control group is sprayed with clear water.
TABLE 2
| Control group | Yue nong Si Miao | |
| Effective ear ratio | 0.75 | 0.89 |
| Setting percentage | 76.4％ | 86.8％ |
TABLE 3
| Control group | Meixiangzhan No. 2 | |
| Effective ear ratio | 0.72 | 0.87 |
| Setting percentage | 54.5％ | 61.3％ |
The experimental results are shown in tables 2 and 3, table 2 shows the influence results of the spraying of the solution of benconazole on the effective panicle rate and the setting percentage of yuenongsimiao silkworms in the field experiment, and table 3 shows the influence results of the spraying of the solution of benconazole on the effective panicle rate and the setting percentage of meixiang account for No. 2 in the field experiment.
In conclusion, the spraying of the benconazole promotes the tillering bud differentiation of the rice, the tillering bud can tillere and grow a plurality of ears, and the yield of the ratoon rice is effectively improved.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.
Claims (10)
1. Application of benzoprazole in promoting rice shoot bud differentiation.
2. Application of benconazole in planting of ratooning rice, which is characterized in that the benconazole is used for promoting the differentiation of the shoot buds of the ratooning rice.
3. A planting method of ratoon rice is characterized by comprising the following steps: the solution of the benazolin is sprayed on the rice stumps.
4. The method according to claim 3, wherein the solvent for preparing the solution of benzoxyazole is at least one selected from the group consisting of absolute ethanol, distilled water, acetone, toluene, xylene.
5. The method as claimed in claim 3, wherein the concentration of the sprayed benzpyrole solution is 10-50 μmol/L.
6. The method as claimed in claim 3, wherein the concentration of the sprayed benzpyrole solution is 15-50 μmol/L.
7. The method as claimed in claim 3, wherein the amount of the solution of benzoxadiazole sprayed is from 15 to 20 liters/acre.
8. The method according to claim 3, further comprising adding or not adding a bactericide, insecticide, acaricide, nematicide, herbicide, plant growth regulator, fertilizer or soil conditioner to the solution of benzoxap.
9. The method according to claim 3, wherein the rice stump height is 10-50 cm.
10. A method for promoting rice shoot differentiation is characterized by comprising the following steps: the solution of benzoprazole was sprayed on rice.
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