CN113142492B - Production process of fermented bean curd and fermented bean curd powder - Google Patents
Production process of fermented bean curd and fermented bean curd powder Download PDFInfo
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Abstract
Description
技术领域technical field
本申请涉及调味品加工的领域,尤其是涉及一种豆腐乳的生产工艺及腐乳粉。The application relates to the field of condiment processing, in particular to a production process of fermented bean curd and fermented bean curd powder.
背景技术Background technique
腐乳是豆腐加入食盐、酒,经长霉、腌胚、发酵制成的奶酪型块状制品,是一种富有营养的蛋白质发酵食品,富含多种氨基酸,具有独特鲜味与酯香风味的佐餐食品,深受人们喜爱。fermented bean curd is a cheese-shaped block product made of tofu by adding salt and wine, growing mold, pickling embryos, and fermenting it. It is loved by people as a side food.
豆腐乳以大豆为主要原料,经过浸泡、磨豆煮浆、制坯、前期发酵、拔毛、后期发酵等工艺,经过密封发酵制成。豆类的主要成分是蛋白质、脂肪和碳水化合物等,前期发酵主要是豆腐胚接种的雅致放射毛霉菌生长繁殖的过程;腌胚,盐能够使霉菌菌丝体中的酶释放出来;后期发酵主要是利用雅致放射毛霉菌分泌的多种酶结合盐酒发生复杂的生化反应,将豆腐胚中的蛋白质分解成肽及氨基酸等物质的过程。Fermented fermented bean curd is made from soybeans as the main raw material, and is made through the processes of soaking, grinding soybeans, boiling, making blanks, pre-fermenting, plucking, and post-fermenting, and then sealed and fermented. The main components of beans are protein, fat and carbohydrates, etc. The early fermentation is mainly the process of growth and reproduction of Mucor radiatae inoculated with tofu embryos; pickled embryos and salt can release the enzymes in the mold mycelium; the later fermentation is mainly It is a process in which the proteins in the tofu embryo are decomposed into peptides and amino acids through complex biochemical reactions using a variety of enzymes secreted by Mucor radiatae combined with salt and wine.
后期发酵的汤料中,酒精含量越高或盐度越高均对蛋白酶作用的抑制越明显,腐孔的成熟期也越长,但盐度降低,又会使成熟腐乳的白斑率增加;发酵温度离蛋白酶等主要酵系的最佳作用温度越近,水解的速度越快,后发酵时间越短;但加快发酵物质水解速度腐乳风味会有欠缺,所以如何提高腐乳的风味、香气和质量,并使其有较好的生产效益,值得进一步研究。In the late-fermented soup, the higher the alcohol content or the higher the salinity, the more obvious the inhibition of the protease action, and the longer the maturity period of the rot pores, but the decrease of the salinity will increase the white spot rate of the mature fermented bean curd; fermentation The closer the temperature is to the optimal temperature of the main fermentation systems such as protease, the faster the hydrolysis rate and the shorter the post-fermentation time; but accelerating the hydrolysis rate of the fermented substances, the flavor of fermented bean curd will be lacking, so how to improve the flavor, aroma and quality of fermented bean curd? And make it have better production efficiency, it is worthy of further study.
发明内容SUMMARY OF THE INVENTION
为了提高腐乳的风味、香气和质量,并使其有较好的生产效益,本申请提供一种豆腐乳的生产工艺及腐乳粉。In order to improve the flavor, aroma and quality of fermented bean curd and make it have better production benefits, the present application provides a production process of fermented bean curd and fermented bean curd powder.
第一方面,本申请提供一种豆腐乳的生产工艺,采用如下的技术方案:First aspect, the application provides a kind of production technology of fermented bean curd, adopts following technical scheme:
一种豆腐乳的生产工艺,包括以下步骤,A production process of fermented bean curd, comprising the following steps,
S1:在豆腐胚上接种含有雅致放射毛霉和少孢根霉的混合菌种,进行前期发酵;S1: Inoculate a mixed strain containing Mucor radiatae and Rhizopus oligospora on the tofu embryos for pre-fermentation;
S2:前期发酵结束后,在10-12℃、湿度45-60%条件下放置24-48h,然后在28-31℃,湿度80-95%放置6-24h,循环2-3次,对豆腐胚进行撮毛;S2: After the pre-fermentation is over, place it at 10-12°C and humidity 45-60% for 24-48h, then place it at 28-31°C and humidity 80-95% for 6-24h, and cycle 2-3 times. Embryos are tufted;
S3:将S2得到的豆腐胚采用分层加盐法腌制2-6天,盐的用量为豆腐胚重量的10-12wt%,然后用3-5wt%的盐水冲洗豆腐胚,放置豆腐胚20-30h;S3: the bean curd embryos obtained in S2 are cured for 2-6 days by layering and salting, and the amount of salt is 10-12wt% of the weight of the bean curd embryos, then rinse the tofu embryos with 3-5wt% salt water, and place the tofu embryos for 20 -30h;
S4:将S3得到的豆腐胚刚好没入汤料中,在22-30℃下后期发酵30-45天。S4: Just submerge the tofu embryo obtained in S3 into the soup, and ferment it for 30-45 days at 22-30°C.
通过采用上述技术方案,在10-12℃、湿度45-60%条件下放置24-48h,为低温环境,在28-31℃,湿度80-95%放置6-24h,为高温环境,一次低温环境和一次高温环境为一个循环;前期发酵后,在交替的低温和高温环境下,混合菌种产生的菌丝能够一定量的渗入豆腐胚中一定的深度,使得豆腐胚的内部和外部均分布一定含量的蛋白酶,在后期发酵过程中,豆腐胚中内外蛋白质的水解速度较为一致,豆腐胚能较快发酵为内外柔软度一致性较高的腐乳,有较好的生产效益,同时提高了腐乳的风味、香气和质量。By adopting the above technical solution, it is placed under the conditions of 10-12°C and humidity of 45-60% for 24-48h, which is a low-temperature environment, and placed at 28-31°C and humidity of 80-95% for 6-24h, which is a high-temperature environment and a low-temperature environment. The environment and a high temperature environment are a cycle; after the early fermentation, under the alternating low temperature and high temperature environment, the mycelium produced by the mixed strains can penetrate a certain amount into the tofu embryo to a certain depth, so that the inside and outside of the tofu embryo are evenly distributed. With a certain content of protease, in the later fermentation process, the hydrolysis rate of internal and external proteins in the tofu embryo is relatively consistent, and the tofu embryo can be fermented quickly into fermented bean curd with high consistency of internal and external softness, which has better production efficiency and improves the bean curd. flavor, aroma and quality.
可选的,所述前期发酵的条件为发酵温度21-28℃,发酵时间26-30h,空气湿度控制60-90%。Optionally, the conditions of the pre-fermentation are a fermentation temperature of 21-28° C., a fermentation time of 26-30 hours, and an air humidity control of 60-90%.
通过采用上述技术方案,该条件下,混合菌种能够使雅致放射毛霉和少孢根霉更好的生长,在豆腐胚上长满菌丝,形成柔软、细密而坚韧的皮膜并积累大量的蛋白酶,有利于提高腐乳的风味和香气。By adopting the above technical solution, under this condition, the mixed strains can make Mucor radiatae and Rhizopus oligospora grow better, cover the tofu embryo with mycelium, form a soft, dense and tough film and accumulate a large amount of Protease helps to improve the flavor and aroma of fermented bean curd.
可选的,所述混合菌种包括按照孢子数比为1:(0.2-0.4)的雅致放射毛霉和少孢根霉。Optionally, the mixed strains include Mucor radioides and Rhizopus oligosporum with a spore number ratio of 1:(0.2-0.4).
通过采用上述技术方案,在该菌种的比例和选择下,其产生的菌丝能够一定量的渗入豆腐胚中一定的深度,豆腐胚中内外蛋白质的水解速度较为一致,豆腐胚能较快发酵为内外柔软度一致性较高的腐乳。By adopting the above technical scheme, under the ratio and selection of the strain, the mycelium produced by the bacteria can penetrate into the tofu embryo to a certain depth, the hydrolysis speed of the internal and external proteins in the tofu embryo is relatively consistent, and the tofu embryo can be fermented faster It is fermented bean curd with high consistency of internal and external softness.
可选的,混合菌种包括以下制备步骤:将混合菌种用蒸馏水稀释至孢子数1×107-1×108个/ml,且混合菌种的接种量为豆腐胚重量的4-8wt%。Optionally, the mixed bacterial species comprises the following preparation steps: the mixed bacterial species is diluted with distilled water to a spore count of 1 × 10 7 -1 × 10 8 /ml, and the inoculation amount of the mixed bacterial species is 4-8wt of the weight of the tofu embryo. %.
通过采用上述技术方案,该浓度和接种量下,接种的菌种量更有利于豆腐胚的发酵。By adopting the above technical scheme, under the concentration and inoculation amount, the amount of inoculated bacteria is more conducive to the fermentation of tofu embryos.
可选的,所述汤料含有以下成分:盐4-7wt%,酒精12.5-13.5wt%,抗氧化剂0.3-0.8wt%,且汤料中包括冰醋酸5-8ml/L。Optionally, the soup contains the following components: 4-7wt% of salt, 12.5-13.5wt% of alcohol, 0.3-0.8wt% of antioxidant, and 5-8ml/L of glacial acetic acid is included in the soup.
通过采用上述技术方案,通过一定含量的抗氧化剂、冰醋酸、酒精和盐的配合,使其能有效抑制氨基酸含量的过度增加,能有效抑制白斑的出现,同时能够在更低的盐度下进行后发酵,并有较快的发酵速度。By adopting the above technical solution, through the combination of a certain content of antioxidants, glacial acetic acid, alcohol and salt, it can effectively inhibit the excessive increase of amino acid content, effectively inhibit the appearance of vitiligo, and at the same time, it can be carried out at a lower salinity. Post-fermentation, and has a faster fermentation speed.
可选的,所述抗氧化剂为大豆异黄酮和茶多酚中的一种或多种。Optionally, the antioxidant is one or more of soybean isoflavones and tea polyphenols.
通过采用上述技术方案,大豆异黄酮和茶多酚为天然抗氧化剂,能有效抑制白斑的出现。By adopting the above technical solution, soybean isoflavones and tea polyphenols are natural antioxidants, which can effectively inhibit the appearance of vitiligo.
可选的,所述汤料包括以下制备步骤:Optionally, the soup material includes the following preparation steps:
A1:将面粉蒸熟晾凉,然后每100g面粉加入1-1.3L常温的水和1-3g酵母,20-28℃下发酵30-60min,水为红曲水,红曲水包括以下制备步骤:红曲米碾碎得到红曲米粉,然后每50g红曲米粉放入1000-1200g清水,煮沸,小火续煮5分钟,过滤去渣,即得红曲水;A1: Steam the flour and let it cool, then add 1-1.3L of room temperature water and 1-3g of yeast per 100g of flour, ferment at 20-28°C for 30-60min, the water is red yeast rice water, and the red yeast rice water includes the following preparation steps : Crush red yeast rice to get red yeast rice flour, then put 1000-1200g of water per 50g of red yeast rice flour, boil, continue to cook on low heat for 5 minutes, filter to remove slag, and get red yeast water;
A2:在步骤A1得到的发酵液体中加入盐、酒、抗氧化剂、冰醋酸,并搅拌均匀,酒可以为黄酒、白酒中的一种或多种。A2: Add salt, wine, antioxidants, and glacial acetic acid to the fermentation liquid obtained in step A1, and stir evenly. The wine can be one or more of yellow rice wine and white wine.
第二方面,本申请提供一种腐乳粉,采用如下的技术方案:Second aspect, the application provides a kind of fermented bean curd powder, adopts following technical scheme:
一种腐乳粉,其由权利要求1-7任意一项一种豆腐乳的生产工艺制得,包括有以下重量份数的原料:腐乳块90-110份,调味料5-10份,油料2-15份,香辛料10-30份。A kind of fermented bean curd powder, which is obtained by the production process of any one of claims 1-7, comprising the following raw materials by weight: 90-110 parts of fermented bean curd blocks, 5-10 parts of seasonings, 2 parts of oil -15 servings, 10-30 servings of spices.
通过采用上述技术方案,腐乳块与调味料、油料和香辛料共同复配,得到的腐乳粉的风味更佳。By adopting the above technical scheme, the fermented bean curd block is compounded with seasoning, oil and spices, and the obtained fermented bean curd powder has better flavor.
可选的,所述调味料包括白糖、味精中的一种或多种,油料包括芝麻油、花生油中的一种或多种,香辛料包括五香粉、花椒粉、辣椒粉、胡椒粉、孜然粉中的一种或多种。Optionally, the seasoning includes one or more of sugar and monosodium glutamate, the oil includes one or more of sesame oil and peanut oil, and the spice includes five-spice powder, pepper powder, chili powder, pepper powder, cumin powder one or more of.
通过采用上述技术方案,多种组合下,能够得到以豆腐乳鲜味与酯香风味为主味的多种风味腐乳调味粉。By adopting the above technical solutions, under various combinations, various flavored fermented bean curd seasoning powders with umami flavor and ester flavor as the main flavors can be obtained.
可选的,腐乳粉包括以下制备步骤:Optionally, the fermented bean curd powder includes the following preparation steps:
B1:将腐乳块搅拌均匀并过滤制得腐乳酱;B1: Stir the fermented bean curd blocks evenly and filter to obtain fermented bean curd sauce;
B2:在腐乳酱中加入调味料、油料、香辛料,在胶体磨进行胶磨得到调味酱;B2: adding seasonings, oils, and spices to the fermented bean curd sauce, and performing rubber grinding in a colloid mill to obtain a seasoning sauce;
B3:将B2得到的胶磨后的调味酱经喷雾干燥得到腐乳粉,喷雾进口温度150-350℃,喷雾出口温度60-120℃。B3: spray-drying the rubber-milled sauce obtained in B2 to obtain fermented bean curd powder, the spray inlet temperature is 150-350°C, and the spray outlet temperature is 60-120°C.
通过采用上述技术方案,在腐乳酱中混合调味料、油料、香辛料并经过胶磨,然后在进行喷雾干燥,能够得到以豆腐乳鲜味与酯香风味为主味的多种风味腐乳调味粉,可广泛应用于豆制食品、酱卤食品、烘焙食品、膨化食品、罐头食品、休闲类食品等,且得到的腐乳粉的腐乳与调味料和香辛料之间混合更加均匀,风味更好。By adopting the above-mentioned technical scheme, seasoning, oil, and spices are mixed in the fermented bean curd sauce and subjected to rubber grinding, and then spray-dried to obtain a variety of flavored fermented bean curd seasoning powder with the main flavors of fermented bean curd umami and ester flavor. It can be widely used in soy food, stewed food, baked food, puffed food, canned food, leisure food, etc., and the obtained fermented bean curd powder is more evenly mixed with seasonings and spices, and has a better flavor.
综上所述,本申请包括以下至少一种有益技术效果:To sum up, the present application includes at least one of the following beneficial technical effects:
通过对豆腐乳的生产工艺的设置,并通过对汤料的设置,减少了白斑的产生,并能够大大降低盐度,豆腐胚的发酵速度较快,同时得到的腐乳内外柔软度一致性较高,提高了豆腐乳的风味和质量,并使其有较好的生产效益的效果。Through the setting of the production process of fermented bean curd and the setting of the soup material, the generation of white spots can be reduced, and the salinity can be greatly reduced. , improve the flavor and quality of fermented bean curd, and make it have the effect of better production efficiency.
具体实施方式Detailed ways
结合以下内容对本申请作进一步详细说明。The present application will be further described in detail in conjunction with the following contents.
冰醋酸,食品级,山东鑫赢舜新材料有限公司;Glacial acetic acid, food grade, Shandong Xinyingshun New Material Co., Ltd.;
大豆异黄酮,食品级,货号Yhskxz,厂家为山东萍聚生物科技有限公司;Soy isoflavones, food grade, product number Yhskxz, the manufacturer is Shandong Pingju Biotechnology Co., Ltd.;
茶多酚,食品级,厂家为徐州丰瑞生物科技有限公司;Tea polyphenols, food grade, the manufacturer is Xuzhou Fengrui Biotechnology Co., Ltd.;
雅致放射毛霉,货号:EY-987J,规格:冻干粉,品牌:上海一研;Yazhi Mucor radiata, item number: EY-987J, specification: freeze-dried powder, brand: Shanghai Yiyan;
少孢根霉,货号:XY-WSW-1270,规格:冻干管,品牌:xuanya,厂家为上海烜雅生物科技有限公司。Rhizopus oligospora, item number: XY-WSW-1270, specification: freeze-dried tube, brand: xuanya, the manufacturer is Shanghai Xuanya Biotechnology Co., Ltd.
实施例1Example 1
一种豆腐乳的生产工艺,包括以下步骤,A production process of fermented bean curd, comprising the following steps,
S1:在豆腐胚上接种含有雅致放射毛霉和少孢根霉的混合菌种,进行前期发酵,前期发酵的条件为发酵温度21℃,发酵时间30h,空气湿度控制60%;其中,混合菌种包括按照孢子数比为1:0.4的雅致放射毛霉和少孢根霉,混合菌种包括以下制备步骤:将混合菌种用蒸馏水稀释至孢子数1×107个/ml,且混合菌种的接种量为豆腐胚重量的8wt%;S1: Tofu embryos were inoculated with mixed strains of Mucor radiatae and Rhizopus oligosporum for pre-fermentation. The conditions of pre-fermentation were fermentation temperature of 21°C, fermentation time of 30h, and air humidity control of 60%; among them, the mixed bacteria The species include Mucor radiotae and Rhizopus oligospora with a spore count ratio of 1:0.4, and the mixed strains include the following preparation steps: dilute the mixed strains with distilled water to 1×10 7 spores/ml, and the mixed strains The inoculum size of the species is 8wt% of the weight of the tofu embryo;
S2:前期发酵结束后,在10℃、湿度60%条件下放置24h,然后在31℃,湿度80%放置24h,循环2次,对豆腐胚进行撮毛;S2: After the pre-fermentation is over, place it at 10°C and 60% humidity for 24 hours, then at 31°C and 80% humidity for 24 hours, cycle 2 times, and pinch the tofu embryos;
S3:将S2得到的豆腐胚采用分层加盐法腌制6天,盐的用量为豆腐胚重量的10wt%,然后用5wt%的盐水冲洗豆腐胚,放置豆腐胚20h;S3: the bean curd embryos obtained in S2 are cured for 6 days by the layered salting method, and the consumption of salt is 10wt% of the weight of the tofu embryos, then rinse the tofu embryos with 5wt% salt water, and place the tofu embryos for 20h;
S4:将S3得到的豆腐胚浸入汤料中,在30℃下后期发酵30天。S4: Immerse the tofu embryos obtained in S3 into the soup stock, and ferment at 30° C. for 30 days.
汤料包括以下制备步骤:The soup stock includes the following preparation steps:
A1:将面粉蒸熟晾凉,然后每100g面粉加入1L水和3g酵母,20℃下发酵60min,水为红曲水,红曲水包括以下制备步骤:红曲米碾碎得到红曲米粉,然后每50g红曲米粉放入1000g清水,煮沸,小火续煮5分钟,过滤去渣,即得红曲水;A1: Steam the flour and let it cool, then add 1L of water and 3g of yeast per 100g of flour, and ferment at 20°C for 60min. The water is red yeast water. The red yeast water includes the following preparation steps: red yeast rice is crushed to obtain red yeast rice flour, Then put 1000g of water per 50g of red yeast rice noodles, boil, continue to cook on low heat for 5 minutes, filter to remove slag, and get red yeast water;
A2:在步骤A1得到的发酵液体中加入盐、酒、抗氧化剂、冰醋酸,使得汤料含有盐4wt%,酒精13.5wt%,抗氧化剂0.3wt%,且每升汤料含有冰醋酸8ml,抗氧化剂为大豆异黄酮。A2: adding salt, wine, antioxidant and glacial acetic acid to the fermentation liquid obtained in step A1, so that the soup contains 4wt% of salt, 13.5wt% of alcohol, 0.3wt% of antioxidant, and each liter of soup contains 8ml of glacial acetic acid, Antioxidants are soy isoflavones.
实施例2Example 2
与实施例1的不同之处在于:The difference from Example 1 is:
S2:前期发酵结束后,在11℃、湿度50%条件下放置32h,然后在29℃,湿度90%放置12h,循环3次,对豆腐胚进行撮毛;S2: After the pre-fermentation is over, place it at 11°C and humidity of 50% for 32h, then at 29°C and humidity of 90% for 12h, cycle 3 times, and pinch the tofu embryos;
S3:将S2得到的豆腐胚采用分层加盐法腌制4天,盐的用量为豆腐胚重量的11wt%,然后用4wt%的盐水冲洗豆腐胚,放置豆腐胚24h。S3: The bean curd embryos obtained in S2 are preserved for 4 days by the method of layering and adding salt, and the amount of salt is 11wt% of the weight of the tofu embryos, and then the tofu embryos are rinsed with 4wt% brine, and the tofu embryos are placed for 24 hours.
S4:将S3得到的豆腐胚浸入汤料中,在25℃下后期发酵32天。S4: Immerse the tofu embryos obtained in S3 into the soup stock, and ferment at 25° C. for 32 days.
汤料包括以下制备步骤:The soup stock includes the following preparation steps:
A1:将面粉蒸熟晾凉,然后每100g面粉加入1.1L水和2g酵母,23℃下发酵40min,水为红曲水,红曲水包括以下制备步骤:红曲米碾碎得到红曲米粉,然后每50g红曲米粉放入1100g清水,煮沸,小火续煮5分钟,过滤去渣,即得红曲水。A1: Steam the flour and let it cool, then add 1.1L of water and 2g of yeast per 100g of flour, ferment at 23°C for 40min, the water is red yeast water, and the red yeast water includes the following preparation steps: red yeast rice is crushed to obtain red yeast rice flour , and then put 1100g of water per 50g of red yeast rice noodles, boil, continue to cook on low heat for 5 minutes, filter to remove slag, and get red yeast water.
步骤A2中,抗氧化剂为重量比为1:1的大豆异黄酮和茶多酚。In step A2, the antioxidant is soybean isoflavones and tea polyphenols in a weight ratio of 1:1.
实施例3Example 3
与实施例1的不同之处在于:The difference from Example 1 is:
S2:前期发酵结束后,在12℃、湿度45%条件下放置48h,然后在28℃,湿度95%放置6h,循环3次,对豆腐胚进行撮毛;S2: After the pre-fermentation is over, place it at 12°C and humidity 45% for 48h, then place it at 28°C and humidity 95% for 6h, cycle 3 times, and pinch tofu embryos;
S3:将S2得到的豆腐胚采用分层加盐法腌制2天,盐的用量为豆腐胚重量的12wt%,然后用3wt%的盐水冲洗豆腐胚,放置豆腐胚30h;S3: the bean curd embryos obtained in S2 are cured by layering and salting for 2 days, and the amount of salt is 12wt% of the weight of the tofu embryos, then rinse the tofu embryos with 3wt% salt water, and place the tofu embryos for 30h;
S4:将S3得到的豆腐胚浸入汤料中,在22℃下后期发酵45天。S4: Immerse the tofu embryos obtained in S3 into the soup stock, and ferment at 22° C. for 45 days.
汤料包括以下制备步骤:The soup stock includes the following preparation steps:
A1:将面粉蒸熟晾凉,然后每100g面粉加入1.3L水和1g酵母,28℃下发酵30min,水为红曲水,红曲水包括以下制备步骤:红曲米碾碎得到红曲米粉,然后每50g红曲米粉放入1200g清水,煮沸,小火续煮5分钟,过滤去渣,即得红曲水。A1: Steam the flour and let it cool, then add 1.3L of water and 1g of yeast per 100g of flour, ferment at 28°C for 30min, the water is red yeast water, and the red yeast water includes the following preparation steps: red yeast rice is crushed to obtain red yeast rice flour , and then put 1200g of water per 50g of red yeast rice noodles, boil, continue to cook on low heat for 5 minutes, filter to remove slag, and get red yeast water.
实施例4Example 4
与实施例2的不同之处在于:前期发酵的条件为发酵温度23℃,发酵时间28h,空气湿度控制75%。The difference from Example 2 is that the conditions of the early fermentation are that the fermentation temperature is 23° C., the fermentation time is 28 hours, and the air humidity is controlled at 75%.
实施例5Example 5
与实施例2的不同之处在于:前期发酵的条件为发酵温度28℃,发酵时间26h,空气湿度控制90%。The difference from Example 2 is that the conditions of the previous fermentation are that the fermentation temperature is 28°C, the fermentation time is 26h, and the air humidity is controlled to 90%.
实施例6Example 6
与实施例4的不同之处在于:混合菌种包括按照孢子数比为1:0.3的雅致放射毛霉和少孢根霉,混合菌种包括以下制备步骤:将混合菌种用蒸馏水稀释至孢子数5×107个/ml,且混合菌种的接种量为豆腐胚重量的6wt%。The difference from Example 4 is that: the mixed strains include Mucor radiotae and Rhizopus oligospora with a spore number ratio of 1:0.3, and the mixed strains include the following preparation steps: dilute the mixed strains with distilled water to spores 5×10 7 cells/ml, and the inoculation amount of mixed strains was 6wt% of the weight of tofu embryos.
实施例7Example 7
与实施例4的不同之处在于:混合菌种包括按照孢子数比为1:0.2的雅致放射毛霉和少孢根霉,混合菌种包括以下制备步骤:将混合菌种用蒸馏水稀释至孢子数1×108个/ml,且混合菌种的接种量为豆腐胚重量的4wt%The difference from Example 4 is that the mixed strains include Mucor radioliquor and Rhizopus oligosporum with a spore number ratio of 1:0.2, and the mixed strains include the following preparation steps: dilute the mixed strains with distilled water to spores A few 1 × 108 /ml, and the inoculum of the mixed strain is 4wt% of the weight of the tofu embryo
实施例8Example 8
与实施例6的不同之处在于:在步骤A1得到的发酵液体中加入盐、酒、抗氧化剂、冰醋酸,使得汤料含有盐5.5wt%,酒精13wt%,抗氧化剂0.6wt%,且每升汤料含有冰醋酸6.5ml。The difference with Example 6 is: in the fermentation liquid obtained in step A1, add salt, wine, antioxidant, glacial acetic acid, so that the soup contains 5.5wt% salt, 13wt% alcohol, 0.6wt% antioxidant, and each One liter of soup stock contains 6.5ml of glacial acetic acid.
实施例9Example 9
与实施例6的不同之处在于:在步骤A1得到的发酵液体中加入盐、酒、抗氧化剂、冰醋酸,使得汤料含有盐7wt%,酒精12.5wt%,抗氧化剂0.8wt%,且每升汤料含有冰醋酸5ml。The difference with Example 6 is: in the fermentation liquid obtained in step A1, add salt, wine, antioxidant, glacial acetic acid, so that the soup contains 7wt% salt, 12.5wt% alcohol, 0.8wt% antioxidant, and each One liter of soup contains 5ml of glacial acetic acid.
实施例10Example 10
与实施例6的不同之处在于:在步骤A1得到的发酵液体中加入盐、酒,使得汤料含有盐5.5wt%,酒精13wt%。The difference from Example 6 is that salt and wine are added to the fermented liquid obtained in step A1, so that the soup contains 5.5 wt % of salt and 13 wt % of alcohol.
实施例11Example 11
一种腐乳粉,其由实施例8得到的腐乳块制得,腐乳粉包括以下制备步骤:A kind of fermented bean curd powder, it is made by the bean curd block obtained in Example 8, and the fermented bean curd powder comprises the following preparation steps:
B1:将腐乳块搅拌均匀并过滤制得腐乳酱;B1: Stir the fermented bean curd blocks evenly and filter to obtain fermented bean curd sauce;
B2:在腐乳酱中加入调味料、油料、香辛料,且腐乳块90份,调味料10份,调味料为重量比为1:0.3的白糖和味精,油料2份,油料为芝麻油,香辛料30份,香辛料为重量比为1:2的花椒粉和辣椒粉,在胶体磨进行胶磨得到调味酱;B2: Add seasonings, oils and spices to the fermented bean curd sauce, 90 parts of fermented bean curd blocks, 10 parts of seasonings, the seasonings are sugar and monosodium glutamate with a weight ratio of 1:0.3, 2 parts of oil, sesame oil and 30 parts of spices , the spices are Chinese prickly ash and chili powder with a weight ratio of 1:2, and the colloid mill is subjected to rubber grinding to obtain the sauce;
B3:将B2得到的胶磨后的调味酱经喷雾干燥得到腐乳粉,喷雾进口温度150℃,喷雾出口温度60℃。B3: The fermented bean curd powder is obtained by spray-drying the rubber-milled sauce obtained in B2, the spray inlet temperature is 150°C, and the spray outlet temperature is 60°C.
实施例12Example 12
与实施例11的不同之处在于:The difference from Example 11 is:
B2:在腐乳酱中加入调味料、油料、香辛料,且腐乳块100份,调味料6份,调味料为重量比为1:0.3的白糖和味精,油料10份,油料为芝麻油,香辛料20份,香辛料为重量比为1:2的花椒粉和辣椒粉,在胶体磨进行胶磨得到调味酱;B2: Add seasonings, oils and spices to the fermented bean curd sauce, 100 parts of bean curd blocks, 6 parts of seasonings, the seasonings are sugar and monosodium glutamate with a weight ratio of 1:0.3, 10 parts of oil, sesame oil and 20 parts of spices , the spices are Chinese prickly ash and chili powder with a weight ratio of 1:2, and the colloid mill is subjected to rubber grinding to obtain the sauce;
B3:将B2得到的胶磨后的调味酱经喷雾干燥得到腐乳粉,喷雾进口温度300℃,喷雾出口温度90℃。B3: The fermented bean curd powder is obtained by spray-drying the rubber-milled sauce obtained in B2, the spray inlet temperature is 300°C, and the spray outlet temperature is 90°C.
实施例13Example 13
与实施例11的不同之处在于:The difference from Example 11 is:
B2:在腐乳酱中加入调味料、油料、香辛料,且腐乳块110份,调味料5份,调味料为重量比为1:0.3的白糖和味精,油料15份,油料为芝麻油,香辛料10份,香辛料为重量比为1:2的花椒粉和辣椒粉,在胶体磨进行胶磨得到调味酱;B2: Add seasonings, oils and spices to the fermented bean curd sauce, and add 110 parts of bean curd blocks, 5 parts of seasonings, and the seasonings are sugar and monosodium glutamate with a weight ratio of 1:0.3, 15 parts of oil, 10 parts of sesame oil and 10 parts of spices , the spices are Chinese prickly ash and chili powder with a weight ratio of 1:2, and the colloid mill is subjected to rubber grinding to obtain the sauce;
B3:将B2得到的胶磨后的调味酱经喷雾干燥得到腐乳粉,喷雾进口温度350℃,喷雾出口温度120℃。B3: The fermented bean curd powder is obtained by spray-drying the gum-milled sauce obtained in B2, the spray inlet temperature is 350°C, and the spray outlet temperature is 120°C.
对比例1Comparative Example 1
与实施例8的不同之处在于:将步骤S1得到的豆腐胚直接采用分层加盐法进行腌制。The difference from Example 8 is that: the bean curd embryo obtained in step S1 is directly salted by layering and salting.
对比例2Comparative Example 2
与实施例8的不同之处在于:步骤S1中,在豆腐胚上仅接种雅致放射毛霉。The difference from Example 8 is that: in step S1, only Mucor radioides is inoculated on the bean curd embryo.
对比例3Comparative Example 3
市售:大块腐乳,品牌:王致和,规格340g。Commercially available: large pieces of fermented bean curd, brand: Wangzhihe, specification 340g.
对比例4Comparative Example 4
市售:红腐乳粉,厂家河南天之宇食品配料有限公司。Commercially available: red bean curd powder, manufacturer Henan Tianzhiyu Food Ingredients Co., Ltd.
对比例5Comparative Example 5
与实施例11的不同之处在于:The difference from Example 11 is:
B2:B1步骤得到的腐乳酱进行胶磨得到调味酱;B2: The fermented bean curd sauce obtained in step B1 is subjected to rubber grinding to obtain seasoning sauce;
B3:将B2得到的胶磨后的调味酱经喷雾干燥得到腐乳粉,再将调味酱与调味料、油料和香辛料混匀。B3: spray-drying the gum-milled seasoning sauce obtained in B2 to obtain fermented bean curd powder, and then mixing the seasoning sauce with seasonings, oils and spices.
性能检测Performance testing
采用SB/T10170-93《腐乳质量标准和检验方法》进行感官品评,选取30个品评师,对实施例1-10和对比例1-3的豆腐乳均进行品评,评分标准如表A,30个品评结果去掉最高分和最低分然后取平均值,品评结果见表1;对实施例11-13、对比例4-5的腐乳粉进行品评,评分标准如表B,30个品评结果去掉最高分和最低分然后取平均值,品评结果见表2;后期发酵结束后,常温继续放置实施例1-10和对比例1-3的豆腐乳1年,观察其白斑在第几个月出现,并记录其出现时间至表1。Adopt SB/T10170-93 "fermented bean curd quality standard and inspection method" to conduct sensory evaluation, select 30 tasters to evaluate the fermented bean curd of Example 1-10 and Comparative Example 1-3, and the scoring standard is as shown in Table A, 30 Remove the highest score and the lowest score for each evaluation result and then take the average value. The evaluation results are shown in Table 1. The fermented bean curd powder of Example 11-13 and Comparative Example 4-5 are evaluated, and the scoring standard is shown in Table B. Then take the average value of points and the lowest score, and the evaluation results are shown in Table 2; After the fermentation in the later stage, the fermented bean curd of Example 1-10 and Comparative Example 1-3 was continued to be placed at room temperature for 1 year, and its vitiligo was observed to appear in the first few months, And record its appearance time to Table 1.
表A腐乳评分标准表Table A fermented bean curd scoring standard table
表1 腐乳品评结果表Table 1 Evaluation results of fermented bean curd
表B腐乳粉评分标准表Table B bean curd powder scoring standard table
表2 腐乳粉品评结果表Table 2 Evaluation results of fermented bean curd powder
根据表1可以看出,实施例1-12和对比例3中,实施例1-12的评分明显优于对比例3,所以本申请的豆腐乳的生产工艺更优。As can be seen from Table 1, in Examples 1-12 and Comparative Example 3, the scores of Examples 1-12 are significantly better than those of Comparative Example 3, so the production process of the fermented bean curd of the present application is better.
实施例1-3中,实施例2得到的豆腐乳的评分最高,且实施例2得到的豆腐乳的质地细腻且中心的边缘部分细腻程度一致,风味更佳,所以实施例2的工艺条件更优。In Examples 1-3, the fermented bean curd obtained in Example 2 had the highest rating, and the fermented bean curd obtained in Example 2 had a fine texture and the same degree of fineness at the edge of the center, and the flavor was better, so the process conditions of Example 2 were better. excellent.
实施例2、4-5中,实施例4得到的豆腐乳的评分最高,且风味更优,说明实施例4的前期发酵条件更优。In Examples 2 and 4-5, the fermented bean curd obtained in Example 4 had the highest score and better flavor, indicating that the early fermentation conditions of Example 4 were better.
实施例4、6-7中,实施例6得到的豆腐乳的评分最高,说明实施例6中菌种的比例选择和接种量更优。In Examples 4 and 6-7, the fermented bean curd obtained in Example 6 had the highest score, indicating that the selection of bacterial species and the amount of inoculation in Example 6 were better.
实施例6、8-9中,实施例8得到的豆腐乳的评分最高,该豆腐乳风味更佳,且1年未出现白斑,说明实施例8中的汤料中盐、酒精、抗氧化剂、冰醋酸的含量更优。实施例8、10中,实施例8得到的豆腐乳的评分明显优于实施例10的豆腐乳,且实施例10出现白斑的日期较早,说明实施例8中的汤料中盐、酒精、抗氧化剂、冰醋酸的含量更优。In Examples 6, 8-9, the fermented bean curd obtained in Example 8 had the highest score, and the fermented bean curd had better flavor, and no white spots appeared in 1 year, indicating that salt, alcohol, antioxidants, The content of glacial acetic acid is better. In Examples 8 and 10, the fermented bean curd obtained in Example 8 scored significantly better than the fermented bean curd of Example 10, and the date of occurrence of vitiligo in Example 10 was earlier, indicating that the salt, alcohol, The content of antioxidants and glacial acetic acid is better.
实施例8、对比例1中,对比例1得到的豆腐乳的评分明显降低,对比例1得到的豆腐乳的的风味交叉,且豆腐乳的质地细腻程度降低,所以本申请的生产工艺更优。实施例8、对比例2中,实施例8得到的豆腐乳的评分更好,说明本申请中混合菌种的选择更优。In Example 8 and Comparative Example 1, the score of the fermented bean curd obtained in Comparative Example 1 was significantly reduced, the flavors of the fermented bean curd obtained in Comparative Example 1 were crossed, and the fineness of the texture of the fermented bean curd was reduced, so the production process of the present application was better. . In Example 8 and Comparative Example 2, the fermented bean curd obtained in Example 8 had a better score, indicating that the selection of mixed strains in this application was better.
根据表2可以看出,实施例11-13和对比例4中,实施例11-13的评分明显优于对比例4,尤其在滋味气味项目评分中,实施例11-13的评分明显优于对比例4,本申请的腐乳粉的配方和工艺更优。As can be seen from Table 2, among Examples 11-13 and Comparative Example 4, the scores of Example 11-13 were significantly better than those of Comparative Example 4, especially in the taste and smell item scoring, the scores of Example 11-13 were significantly better than those of Comparative Example 4. In Comparative Example 4, the formula and process of the fermented bean curd powder of the present application are better.
实施例11-13和对比例5中,调味料、油料和香辛料的混入不步骤不同,实施例11-13的评分明显优于对比例5,在滋味气味方面,实施例11-13的评分明显优于对比例5,本申请的腐乳粉的工艺更优。In Examples 11-13 and Comparative Example 5, the mixing steps of seasonings, oils and spices were different, and the scores of Example 11-13 were significantly better than those of Comparative Example 5. In terms of taste and smell, the scores of Example 11-13 were obvious. Compared with Comparative Example 5, the process of the fermented bean curd powder of the present application is better.
以上均为本申请的较佳实施例,并非依此限制本申请的保护范围,故:凡依本申请的结构、形状、原理所做的等效变化,均应涵盖于本申请的保护范围之内。The above are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. Therefore: all equivalent changes made according to the structure, shape and principle of the present application should be covered within the scope of the present application. Inside.
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