CN113142304A

CN113142304A - Probiotic composition for promoting human body metabolism to generate yellow feces and application thereof

Info

Publication number: CN113142304A
Application number: CN202110335206.XA
Authority: CN
Inventors: 杨玲; 贾洪利; 仵红岩; 赵林森; 严超; 郭润晴; 孙策; 齐世华; 张士成; 路江浩
Original assignee: Hebei Inatural Biotechnology Co ltd
Current assignee: Hebei Inatural Biotechnology Co ltd
Priority date: 2021-03-29
Filing date: 2021-03-29
Publication date: 2021-07-23

Abstract

The invention discloses a probiotic composition for promoting human body metabolism to generate yellow feces, which comprises streptococcus thermophilus S7094-7 g, lactobacillus bulgaricus 4-7g, lactobacillus paracasei L57812-17 g, lactobacillus rhamnosus LR 5198-13 g, lactobacillus acidophilus La 288-12 g, lactobacillus helveticus L5513-7 g and bifidobacterium 13-20 g. The probiotic composition for promoting human body metabolism to generate the excrement is adopted, and the application of the probiotic composition can improve the human body metabolism and maintain the balance of intestinal flora.

Description

Probiotic composition for promoting human body metabolism to generate yellow feces and application thereof

Technical Field

The invention relates to the technical field of probiotic compositions, in particular to a probiotic composition for promoting human body metabolism to generate excrement and urine and application thereof.

Background

Stool, which was previously considered to be an inelegant and disconcerting topic, has begun to be widely recognized and discussed as the understanding of the intestinal function increases. Feces is an index of physical health, and feces of healthy people should be banana type and the best type of golden feces.

The morphology of feces is not only related to the physical and mental status and daily life of an individual, but also more important to the health of the intestinal tract. The shape and the color of the excrement are reference indexes for visually reflecting whether the intestinal tract is healthy, and when the intestinal tract flora is in a balanced state, the color of the excrement is golden yellow. Once the intestinal bacteria balance the collapsing disc, the risk of large intestine cancer, colitis, and polyp will increase. The bacteria in the intestinal tract are divided into three groups: good bacteria, bad bacteria and neutral bacteria, and when the three are in a balanced healthy state, high-quality excrement can be discharged.

Disclosure of Invention

The invention aims to provide a probiotic composition for promoting human body metabolism to generate excrement and application thereof, which can improve human body metabolism and maintain intestinal flora balance.

In order to achieve the aim, the invention provides a probiotic composition for promoting human body metabolism to produce yellow feces, which comprises streptococcus thermophilus S7094-7 g, lactobacillus bulgaricus 4-7g, lactobacillus paracasei L57812-17 g, lactobacillus rhamnosus LR 5198-13 g, lactobacillus acidophilus La 288-12 g, lactobacillus helveticus L5513-7 g and bifidobacterium 13-20 g;

streptococcus thermophilus S709 is preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO.18028 and the preservation date of 2019.6.26.

Lactobacillus paracasei L578 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC NO.15603 and the preservation date of 2018.4.11.

Lactobacillus rhamnosus LR519, published in patent application No. 201911301091.1, with accession number CGMCC No. 15969.

The preservation number of lactobacillus acidophilus La28 is CGMCC No.11506, and the preservation unit is as follows: china general microbiological culture Collection center (CGMCC), preservation date 2015.10.15.

The Swiss lactobacillus L551 is preserved in the China general microbiological culture Collection center with the preservation number of CGMCC NO.15604 and the preservation date of 2018.4.11.

Bifidobacterium bifidum TMC3115 with preservation number CGMCC No. 8462.

Bifidobacterium longum L693 with preservation number CGMCC No.19469 and preservation date 2020.3.13.

The above strains are all disclosed in the related patent application documents.

Bifidobacterium lactis BAL531, deposit name: the general microbiological center of China Committee for culture Collection of microorganisms, the address of the collection unit is No. 3 Siro No.1 of Beijing, Chaoyang district, the strain state is survival, and the classification is named: bifidobacterium lactis, deposit number: CGMCC N o.17329, and the preservation date is 3 months and 13 days in 2019.

Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus rhamnosus and Streptococcus thermophilus are fermented simultaneously, Lactobacillus bulgaricus and Lactobacillus helveticus are fermented simultaneously, and then the fermentation products of the Lactobacillus paracasei, the Lactobacillus acidophilus, the Lactobacillus rhamnosus and the Lactobacillus helveticus are mixed and independently added with bifidobacteria to form the composition.

Preferably, the composition comprises Streptococcus thermophilus S7094 g, Lactobacillus bulgaricus 5g, Lactobacillus paracasei L57813 g, Lactobacillus rhamnosus LR 51910 g, Lactobacillus acidophilus La 288 g, Lactobacillus helveticus L5514 g and Bifidobacterium 13 g.

Preferably, the composition comprises Streptococcus thermophilus S7095 g, Lactobacillus bulgaricus 5g, Lactobacillus paracasei L57815 g, Lactobacillus rhamnosus LR 51913 g, Lactobacillus acidophilus La 2810 g, Lactobacillus helveticus L5515 g and Bifidobacterium 15 g.

Preferably, the Bifidobacterium includes Bifidobacterium bifidum TMC 31158-11 g, Bifidobacterium lactis L5314-6g, and Bifidobacterium longum L6931-3 g.

Preferably, the Bifidobacterium includes Bifidobacterium bifidum TMC 311510 g, Bifidobacterium lactis L5315g, and Bifidobacterium longum L6931.25g.

Preferably, the Bifidobacterium includes Bifidobacterium bifidum TMC 31158 g, Bifidobacterium lactis L5316 g, Bifidobacterium longum L6931 g.

Preferably, the Bifidobacterium includes Bifidobacterium bifidum TMC 311511 g, Bifidobacterium lactis L5314 g, Bifidobacterium longum L6933 g.

Each yoghurt comprises 850-960kg of milk, 50-90kg of white sugar, 3-6kg of inulin, 1-3kg of stachyose, 9-12kg of cream, 8-13kg of concentrated whey protein WPC-80 and 700-900g of the probiotic composition.

Preferably, each yoghurt comprises 904kg of milk, 70kg of white sugar, 4kg of inulin, 2kg of stachyose, 10kg of cream, 10kg of concentrated whey protein WPC-80 and 750g of the probiotic composition.

A preparation method of yoghourt for promoting human body metabolism to generate golden stool comprises the following steps:

sequentially adding milk, white sugar, concentrated whey protein WPC-80, cream, inulin, and stachyose into a mixing tank, stirring, and standing at 60 deg.C for 15 min; homogenizing under 18-20 MPa; sterilizing at 95 deg.C for 5min, cooling, adding probiotic composition, stirring, and fermenting at 37 deg.C; stopping fermentation when the acidity reaches 90-100T to obtain fermentation liquor;

the application of a probiotic composition for promoting human body metabolism to generate golden stool in food, wherein the mixed probiotic dosage form comprises powder and granules, and the preparation method comprises the following steps:

a. sequentially adding milk powder, concentrated whey protein, inulin and stachyose into a mixing tank, stirring, and standing at 60 deg.C for 15 min; homogenizing under 18-20 MPa; sterilizing at 95 deg.C for 5min, cooling, adding probiotic composition, stirring, and fermenting at 37 deg.C; stopping fermentation when the acidity reaches 90-100T to obtain fermentation liquor;

b. deep cooling granulation: dripping liquid bacteria liquid into liquid nitrogen, and instantly cooling to-193 ℃;

freeze-drying: vacuumizing and freeze-drying at the temperature of-20 to-70 ℃ to obtain the probiotic powder or granular preparation.

The probiotic composition dosage form comprises any one of powder, granules, tablets or capsules.

The probiotic powder and the preparation can be directly used, or added into food for eating, such as being mixed with conventional auxiliary materials of the food, or added into food raw materials for eating. Such as: mixing probiotic bacteria or preparation with prebiotics, dietary fiber, etc., and making into food or health food in the form of powder, tablet, granule, crystal ball, block, stick, capsule, etc.

Therefore, by adopting the probiotic composition for promoting human body metabolism to generate the golden feces and the application thereof, after a human body drinks the yoghourt, the number of bifidobacteria and lactobacilli is obviously increased, the number of harmful bacteria is reduced, and the effect of maintaining the balance of intestinal flora can be reflected by improving the color of the feces, and after the yoghourt is drunk, the feces is obviously golden yellow, and the shape of the feces is spiral; the number of living bifidobacteria is kept at a higher level in the shelf life by optimizing the ingredients.

When the daily consumption of the yoghourt is more than 660ml, the effect of the yoghourt can be achieved when the yoghourt is eaten for 2 days, and the optimal type golden stool can be metabolized by a human body. The fermentation process of probiotics in yogurt is more than 18h, and the total viable count of the bacteria powder in the probiotic composition is more than or equal to 5.0 x 10¹⁰CFU/g, wherein the number of bifidobacteria is not less than 2.0 × 10¹⁰CFU/g。

The bifidobacteria and the lactobacilli can improve the color of the excrement, and mainly lie in the following aspects:

(1) promoting repair and maintaining normal intestinal flora to perform normal physiological functions;

(2) reducing the generation source of beta-glucuronidase and the enterohepatic circulation of bilirubin, and directly discharging the bilirubin out of the body;

(3) the acetic acid and lactic acid generated by metabolism reduce the pH value of the intestinal environment, maintain the acidic environment of the intestinal tract, increase the osmotic pressure in the field, increase the water secretion, accelerate the intestinal peristalsis and effectively promote the excretion of bilirubin.

The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.

Drawings

FIG. 1 is a schematic view of a fecal color chart;

FIG. 2 is a statistical analysis of stool color scores before and after trial administration by a test population;

FIG. 3 is a graph showing the total abundance of Bifidobacterium in the feces of the trial population;

FIG. 4 shows the detailed changes of the abundance of Bifidobacterium in the feces of the trial population;

FIG. 5 shows the total abundance changes of Bifidobacterium adolescentis in feces of trial population before and after;

FIG. 6 shows the specific variation of the pre-and post-adolescent abundance of Bifidobacterium in human feces for a trial population;

FIG. 7 is the total change in Lactobacillus pre-post abundance in feces of trial populations;

FIG. 8 shows the specific changes in the pre-and post-lactobacillus abundance in the feces of the trial population;

FIG. 9 is a graph of the gross change in abundance before and after Prevotella in the feces of the trial population;

FIG. 10 is a graph showing the specific change in pre-and post-abundance of Prevotella in the feces of the trial population;

FIG. 11 is a graph showing the total abundance change of Bacteroides thetaiotaomicron in feces of a trial population;

FIG. 12 shows the detailed changes in the pre-and post-natal abundance of Bacteroides thetaiotaomicron in feces from the trial population;

FIG. 13 is a graph of the total change in pre-and post-natal abundance of Clostridium mollicutes in feces from a trial population;

FIG. 14 is a graph showing the specific change in the pre-and post-natal abundance of Clostridium mollicutes in feces of a trial population;

FIG. 15 shows the total abundance of E.coli in human feces before and after sampling;

FIG. 16 shows the concrete changes of the E.coli abundance in the feces of the trial population;

FIG. 17 is a graph of the total change in fecal abundance of enterococcus faecalis in trial populations;

FIG. 18 is a graph showing the specific change in the pre-and post-fecal abundance of enterococcus faecalis among the human trial populations;

FIG. 19 shows the strain changes in the case of cold storage of yogurt;

FIG. 20 is a graph showing fermentation acidity curves of different strains.

Detailed Description

The technical solution of the present invention is further illustrated by the accompanying drawings and examples.

A probiotic composition for promoting metabolism of human body to produce stool and urine comprises Streptococcus thermophilus S7094-7 g, Lactobacillus bulgaricus 4-7g, Lactobacillus paracasei L57812-17 g, Lactobacillus rhamnosus LR 5198-13 g, Lactobacillus acidophilus La 288-12 g, Lactobacillus helveticus L5513-7 g and Bifidobacterium 13-20 g.

The lactobacillus paracasei, the lactobacillus acidophilus, the lactobacillus rhamnosus and the streptococcus thermophilus have no great difference in fermentation curves and extreme post-acidification, can be fermented simultaneously, and the lactobacillus bulgaricus and the lactobacillus helveticus have stronger extreme post-acidification and are added in a small amount when being fermented together with the lactobacillus paracasei, the lactobacillus acidophilus, the lactobacillus rhamnosus and the streptococcus thermophilus; the fermentation temperature is 37 ℃, and the final acidity is 90-100 DEG T. The Bifidobacterium is fermented alone at 37 deg.C, and added to form the composition after terminating acidity of 70 ° T.

(1) In order to simplify the fermentation process, the mixed fermentation is performed on the bacteria with consistent fermentation acid production rate, and as can be seen from fig. 20, the fermentation rate of the bifidobacteria is slow and the acid is extremely weak after fermentation, while the bifidobacteria and the metabolites thereof are key strains for improving the color of the feces, so that the bifidobacteria need to be fermented independently in order to ensure the normal fermentation of the bifidobacteria and maximize the production of the metabolites.

(2) The lactobacillus paracasei, the lactobacillus acidophilus and the lactobacillus rhamnosus have no great difference in fermentation curves and extremely post-acid, can be fermented simultaneously, and the lactobacillus bulgaricus and the lactobacillus helveticus have strong extremely post-acid and are added in a small amount when being fermented together with the lactobacillus paracasei, the lactobacillus acidophilus and the lactobacillus rhamnosus.

s1, adding milk, white sugar, concentrated whey protein WPC-80, cream, inulin and stachyose into a mixing tank in sequence, stirring uniformly, and standing at 60 deg.C for 15 min;

s2, homogenizing the substance obtained in the step S1 under 18-20 MPa;

s3, after homogenizing, sterilizing at 95 ℃ for 5min, cooling, adding the probiotic composition, stirring uniformly, and fermenting at 37 ℃;

s4, stopping fermentation when the acidity of the fermentation material is 90-100T in the step S3, rotating at 1000rpm for 3min, and refrigerating at 4 ℃.

Example one

The composition comprises Streptococcus thermophilus S7094 g, Lactobacillus bulgaricus 5g, Lactobacillus paracasei L57813 g, Lactobacillus rhamnosus LR 51910 g, Lactobacillus acidophilus La 288 g, Lactobacillus helveticus L5514 g and Bifidobacterium 13 g.

Example two

The composition comprises Streptococcus thermophilus S7095 g, Lactobacillus bulgaricus 5g, Lactobacillus paracasei L57815 g, Lactobacillus rhamnosus LR 51913 g, Lactobacillus acidophilus La 2810 g, Lactobacillus helveticus L5515 g and Bifidobacterium 15 g.

Implementation III

The Bifidobacterium includes Bifidobacterium bifidum TMC 31158-11 g, Bifidobacterium lactis L5314-6g, and Bifidobacterium longum L6931-3 g.

Example four

The Bifidobacterium includes Bifidobacterium bifidum TMC 311510 g, Bifidobacterium lactis L5315g, and Bifidobacterium longum L6931.25g.

EXAMPLE five

The Bifidobacterium includes Bifidobacterium bifidum TMC 31158 g, Bifidobacterium lactis L5316 g, and Bifidobacterium longum L6931 g.

EXAMPLE six

The Bifidobacterium includes Bifidobacterium bifidum TMC 311511 g, Bifidobacterium lactis L5314 g, and Bifidobacterium longum L6933 g.

EXAMPLE seven

Each yoghurt comprises 904kg of milk, 70kg of white sugar, 4kg of inulin, 2kg of stachyose, 10kg of cream, 10kg of concentrated whey protein WPC-80 and 750g of the probiotic composition.

Example eight

The probiotic powder and the preparation can be directly used or added into other food for eating.

Example nine

Mixing probiotic bacteria or preparation with food materials such as prebiotics and dietary fiber, and making into food or health food in the form of powder, tablet, granule, crystal ball, block, stick, capsule, etc.

Test 1

1. Changes in stool color of trial population

Selecting healthy people with regular work and rest diet, no special diet, no medicine or other probiotic products to perform trial test, and finally grouping 10 people; the test taker drinks 1 bottle (300 mL in total) of yoghourt in the morning and afternoon each day, drinks 600mL in total each day, continuously takes the test for two days, fills in a questionnaire before and after the test taking is finished, observes and records the stool character within two days, takes the stool character before the test taking as a reference, and compares the stool character improvement conditions of the test taker. The color and the character of the excrement are formulated according to the standard of excrement book, and the color of the excrement is as follows: 1-14 points, referring to the questionnaire color chart, the closer the color is to the golden color, the smaller the score, as shown in fig. 1. The statistics of the fecal color scores before and after trial are shown in table 1 and fig. 2.

TABLE 1 statistical analysis of stool color scores before and after fitting

According to the statistical analysis of the color scores of the feces, the change of the color of the feces before and after trial clothes is found to have extremely significant difference, which shows that the color of the feces after trial clothes is obviously improved.

2. Detection of intestinal flora in trial population

The intestinal flora detection is carried out on the people trying to take the yoghourt, and the fecal samples before and after 2 days of continuous trial taking are compared and analyzed, and the results are shown in the table.

Before and after the yogurt is tried, beneficial bacteria bifidobacterium generally shows an ascending trend, and bifidobacterium adolescentis show an ascending trend on individuals without significance;

the lactobacillus has ascending trend on the whole and the individual to reach a significant level, and has extremely significant difference;

the general trend of the Prevotella is increased, and the individual trend is increased;

the Bacteroides thetaiotaomicron generally has an ascending trend, and the Bacteroides thetaiotaomicron generally has an ascending trend on individuals;

the clostridium tender has an overall ascending trend without obvious difference, and the clostridium tender presents an individual ascending trend;

harmful bacteria escherichia coli and enterococcus faecalis present a downward trend; salmonella and Shigella were not detected before and after drinking.

Through the detection and conjecture of the flora, the fermented yoghourt can promote the proliferation of beneficial bacteria of a human body and reduce the generation of harmful bacteria. After taking yoghurt, lactobacillus rose significantly, probably a key strain causing stool color change;

through the detection and conjecture of the flora, the fermented yoghourt can promote the proliferation of beneficial bacteria of a human body and reduce the generation of harmful bacteria. After the fermented yoghourt is taken, lactobacillus is remarkably increased and possibly is a key strain causing the color change of excrement, so that the addition amount of the lactobacillus is ensured in the preparation process of the yoghourt. The results of questionnaires also show that the population with obviously improved stool color is the population with increased bifidobacteria in intestinal tract, and the bifidobacteria is verified to be the key strain causing golden stool in human bodies.

Therefore, the existence of beneficial bacteria in the intestinal tract can regulate the balance of intestinal flora; in combination with stool color survey statistics, bifidobacteria and lactobacilli are key strains causing stool color improvement.

Test 2

The number of viable bacteria in the golden fecal yoghourt shelf life at 4 ℃ is tracked, and the results are as follows:

viable count CFU/mL	Initial	3 days	7 days	14 days	21 days
						Total number of viable bacteria	1.25×10⁹	1.05×10⁹	1.24×10⁹	9.1×10⁸	8.2×10⁸
Bifidobacterium	7.35×10⁷	4.05×10⁷	1.76×10⁷	1×10⁶	5.4×10⁵
						Rhamnose and paracasea	2.52×10⁸	2.5×10⁸	2×10⁸	1.9×10⁸	1.84×10⁸
Acidophilic, thermophilic, Switzerland	9.24×10⁸	7.59×10⁸	1.02×10⁹	7.1×10⁸	6.3×10⁸

Therefore, the probiotic composition for promoting human body metabolism to generate the yellow feces and the application thereof can improve the human body metabolism and maintain the balance of intestinal flora.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.

Claims

1. A probiotic composition for promoting human body metabolism to produce yellow feces, which is characterized in that: the composition comprises Streptococcus thermophilus S7094-7 g, Lactobacillus bulgaricus 4-7g, Lactobacillus paracasei L57812-17 g, Lactobacillus rhamnosus LR 5198-13 g, Lactobacillus acidophilus La 288-12 g, Lactobacillus helveticus L5513-7 g and Bifidobacterium 13-20 g;

2. The probiotic composition for promoting human body metabolism to produce yellow feces according to claim 1, wherein: the composition comprises Streptococcus thermophilus S7094 g, Lactobacillus bulgaricus 5g, Lactobacillus paracasei L57813 g, Lactobacillus rhamnosus LR 51910 g, Lactobacillus acidophilus La 288 g, Lactobacillus helveticus L5514 g and Bifidobacterium 13 g.

3. The probiotic composition for promoting human body metabolism to produce yellow feces according to claim 1, wherein: the composition comprises Streptococcus thermophilus S7095 g, Lactobacillus bulgaricus 5g, Lactobacillus paracasei L57815 g, Lactobacillus rhamnosus LR 51913 g, Lactobacillus acidophilus La 2810 g, Lactobacillus helveticus L5515 g and Bifidobacterium 15 g.

4. The probiotic composition for promoting human body metabolism to produce yellow feces according to claim 1, wherein: the Bifidobacterium includes Bifidobacterium bifidum TMC 31158-11 g, Bifidobacterium lactis L5314-6g, and Bifidobacterium longum L6931-3 g.

5. The probiotic composition for promoting human body metabolism to produce yellow feces according to claim 1, wherein: the Bifidobacterium includes Bifidobacterium bifidum TMC 311510 g, Bifidobacterium lactis L5315g, and Bifidobacterium longum L6931.25g.

6. The probiotic composition for promoting human body metabolism to produce yellow feces according to claim 1, wherein: the Bifidobacterium includes Bifidobacterium bifidum TMC 31158 g, Bifidobacterium lactis L5316 g, and Bifidobacterium longum L6931 g.

7. The probiotic composition for promoting human body metabolism to produce yellow feces according to claim 1, wherein: the Bifidobacterium includes Bifidobacterium bifidum TMC 311511 g, Bifidobacterium lactis L5314 g, and Bifidobacterium longum L6933 g.

8. A yoghourt for promoting human body metabolism to generate yellow feces is characterized in that: each yoghurt comprises 850-960kg of milk, 50-90kg of white sugar, 3-6kg of inulin, 1-3kg of stachyose, 9-12kg of cream, 8-13kg of concentrated whey protein WPC-80 and 700-900g of the probiotic composition.

9. The yogurt for promoting human body metabolism to produce golden stool according to claim 8, wherein: each yoghurt comprises 904kg of milk, 70kg of white sugar, 4kg of inulin, 2kg of stachyose, 10kg of cream, 10kg of concentrated whey protein WPC-80 and 750g of the probiotic composition.

10. The use of a probiotic composition for promoting the human body to metabolize to produce yellow feces according to any one of claims 1 to 9 in food, wherein the mixed probiotic dosage form comprises powder and granules, and the preparation method comprises the following steps: