CN113122428A - Apoptosis induction detection device and application method thereof - Google Patents
Apoptosis induction detection device and application method thereof Download PDFInfo
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Abstract
本发明涉及细胞检测技术领域，具体涉及一种细胞凋亡诱导检测装置及其使用方法，包括支撑模块，所述支撑模块固定连接有用于输送试剂的送液模块与用于吸取废液的吸取模块，所述支撑模块包括储液盒一，所述储液盒一顶面固定连接有连接环，所述连接环侧面设置有与支撑模块固定连接的支撑机构。本发明中，通过储液盒一设置有六个隔板，可以将不同的试剂分别添加到储液盒一的六个空间内，可以启动气泵一，气泵一可以通过连接管与相应的进气槽向储液盒一内相应的空间注气，从而将相应的试剂通过送液管压出出液槽一，进行试剂的加注，这样不需要实验人员手动吸取加注试剂，操作简单，有利于实验的进行。
The invention relates to the technical field of cell detection, in particular to a cell apoptosis induction detection device and a method for using the same, comprising a support module, wherein the support module is fixedly connected with a liquid supply module for transporting reagents and a suction module for sucking waste liquid The support module includes a liquid storage box 1, the top surface of the liquid storage box 1 is fixedly connected with a connecting ring, and the side surface of the connecting ring is provided with a support mechanism fixedly connected with the support module. In the present invention, since the first liquid storage box is provided with six partitions, different reagents can be added to the six spaces of the first liquid storage box, and the first air pump can be activated, and the first air pump can be connected to the corresponding air intake through the connecting pipe. The tank injects gas into the corresponding space in the liquid storage box 1, so that the corresponding reagent is pressed out of the liquid outlet tank 1 through the liquid feeding pipe, and the reagent is filled, so that the experimenter does not need to manually suck and fill the reagent. conducive to the conduct of experiments.
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a cell apoptosis induction detection device and a using method thereof.
Background
Apoptosis refers to the autonomous and ordered death of cells under the control of genes in order to maintain homeostasis, and is not a passive process but an active process. In biomedical research, it is sometimes necessary to induce apoptosis and then detect apoptosis of cells to observe the apoptosis.
In the experiment of cell apoptosis induction detection, need the experimenter to add various reagents in proper order in the cell culture dish, carry out the experiment of cell apoptosis induction detection, because the reagent number of times that adds in the experiment is more, the experimenter needs frequent reagent that adds like this for the experiment is comparatively troublesome, is unfavorable for going on of experiment.
Disclosure of Invention
In order to overcome the technical problems, the invention aims to provide a cell apoptosis induction detection device and a use method thereof.
The purpose of the invention can be realized by the following technical scheme:
a detection device for apoptosis induction comprises a support module, wherein the support module is fixedly connected with a liquid feeding module for conveying a reagent and a suction module for sucking waste liquid, the support module comprises a first liquid storage box, the top surface of the first liquid storage box is fixedly connected with a connecting ring, the side surface of the connecting ring is provided with a support mechanism fixedly connected with the support module, the support mechanism can support the first liquid storage box through the connecting ring, the central position of the inner side surface of the first liquid storage box is fixedly connected with a fixed rod, the side surface of the fixed rod is fixedly connected with six partition plates fixedly connected with the inner side surface of the first liquid storage box in an annular equidistant manner, the partition plates can separate six spaces from the first liquid storage box, so that different reagents can be conveniently stored, the top surface of the first liquid storage box is positioned between two adjacent partition plates and is fixedly connected with a liquid feeding pipe, the utility model discloses a reagent injection test device, including a circular slot, a supporting mechanism, a gas inlet groove, a circular slot medial surface fixedly connected with plectane I, a plectane top surface corresponds and has all been seted up the gas inlet groove between two baffles, plectane top surface is provided with rotates the plectane two of being connected with the circular slot, the corresponding gas inlet groove intercommunication that is located absorption module one side of plectane two top surfaces has the connecting pipe, supporting mechanism fixedly connected with air pump I, air pump one is given vent to anger end and connecting pipe intercommunication, and air pump one can press into stock solution box one with the gas inlet groove through the connecting pipe in, can press corresponding reagent through the feed pipe out stock solution box one like this, carries out the filling of reagent, does not need frequent manual the absorption of.
Further, the method comprises the following steps: the support module includes the bottom plate, two equal fixedly connected with side lever in long limit of bottom plate top surface, two the relative medial surface sliding connection of side lever has the slide bar, and the slide bar can be followed the side lever and slided, T type groove has been seted up to the slide bar top surface, T type groove medial surface sliding connection has T type piece, T type piece top surface fixedly connected with and the box of placing of slide bar top surface contact, place box inside face sliding connection and have the experiment box, place the box and can follow the side lever and slide with the slide bar, the convenient square that will experiment the box removes to corresponding liquid feed pipe bottom like this, is convenient for add reagent in the square of different experiment boxes.
Further, the method comprises the following steps: the air cylinders are fixedly connected to four corners of the inner bottom surface of the placing box, the output ends of the air cylinders are fixedly connected with the lifting plate which is in contact with the bottom surface of the experiment box, and the air cylinders can lift the experiment box, so that the experiment box is separated from the placing box, and the experiment box can be taken out conveniently.
Further, the method comprises the following steps: the supporting mechanism comprises a baffle frame, two sides of the outer side surface of the baffle frame are fixedly connected with a first supporting frame fixedly connected with the top surfaces of adjacent side rods, the first supporting frame can support the baffle frame, the top surface of the baffle frame is fixedly connected with a top plate, the inner side surface of the top plate is rotatably connected with the outer side surface of a connecting ring, the connecting ring can support a first liquid storage box, the top surface of the top plate is fixedly connected with a fixing frame, the top surface of the fixing frame is fixedly connected with a base of an air pump, the top surface of the fixing frame is fixedly connected with a connecting rod fixedly connected with the inner bottom surface of the fixing frame, the connecting rod can limit the position of a second circular plate, so that the second circular plate can not rotate along with the first liquid storage box, the first liquid storage box can be rotated, different air inlet grooves correspond to, different reagents were filled.
Further, the method comprises the following steps: the utility model discloses a liquid storage box, including go-between top, roof, locating piece, stopper bottom surface, go-between top, roof top surface, fixing groove, stopper bottom surface, stopper card, locating piece, go-between top surface, bottom surface.
Further, the method comprises the following steps: the top surface of the circular plate II is provided with a liquid adding groove corresponding to one air inlet groove, the inner side surface of the liquid adding groove is provided with a first blocking block, the first blocking block can be opened, and reagents are added into the first liquid storage box through the liquid adding groove and the air inlet groove.
Further, the method comprises the following steps: an outer lateral wall annular equidistance fixedly connected with of stock solution box and adjacent liquid delivery pipe lateral wall fixed connection's fixed block, the fixed block can support the liquid delivery pipe.
Further, the method comprises the following steps: the absorption module comprises a second liquid storage box, two sides of the bottom of the two outer side surfaces of the second liquid storage box are fixedly connected with second support frames fixedly connected with the top surfaces of the adjacent side rods, the top surfaces of the second liquid storage box are communicated with an absorption pipe, the top surfaces of the second liquid storage box are fixedly connected with a second air pump, the air inlet end of the second air pump is communicated with the top surfaces of the second liquid storage box, the placing box can be moved to the bottom of the absorption pipe, so that corresponding squares of the experiment box correspond to the absorption pipe, then an air cylinder is started, the experiment box can be moved upwards through a lifting plate by the air cylinder, the bottom end of the absorption pipe is positioned at the bottom of the squares of the experiment box, then the second air pump can be started, the second air pump can suck reagents into the second liquid storage box through the absorption pipe, waste liquid in the squares of the experiment box can be conveniently extracted, the operation is simple, the second blocking block can be opened to discharge the waste liquid in the second liquid storage box.
A use method of a cell apoptosis induction detection device comprises the following specific use operation steps:
the method comprises the following steps: opening the first blocking block, filling reagents into the first liquid storage box through the liquid adding groove and the air inlet groove, and then rotating the connecting ring through the positioning block to rotate the first liquid storage box, so that other air inlet grooves correspond to the liquid adding groove, and different reagents are added into different spaces in the first liquid storage box;
step two: after reagent is added, the placing box can be moved, squares of different experiment boxes correspond to the bottom of the liquid feeding pipe positioned on one side of the connecting pipe, then the first air pump is started, the first air pump can press the reagent corresponding to the space in the first liquid storage box into the squares of the experiment boxes through the liquid feeding pipe, the reagent is filled, the first liquid storage box can be rotated, and the reagent in other spaces in the first liquid storage box is filled into the squares of the experiment boxes through the first air pump;
step three: the placing box can be moved to the bottom of the suction pipe, the air cylinder is started, the air cylinder can enable the experiment box to move upwards, so that the bottom end of the suction pipe is located at the bottom of a square of the experiment box, then the air pump II can be started, the air pump II can suck the reagent into the liquid storage box II through the suction pipe, and other squares of the experiment box can be aligned to the suction pipe to extract the reagent;
step four: after the whole suction discharge square of reagent, can continue to add other reagents through sending liquid module, after the experiment, can start the cylinder, release the experiment box and place the box.
The invention has the beneficial effects that:
1. the side surface of the fixed rod is fixedly connected with six clapboards fixedly connected with the inner side surface of the liquid storage box at an annular equal distance, the top surface of the liquid storage box is fixedly connected with a liquid feeding pipe, the top surface of the circular plate I is provided with an air inlet groove, the top surface of the circular plate I is provided with a circular plate II, the top surface of the circular plate II is communicated with a connecting pipe, the air outlet end of the air pump I is communicated with the connecting pipe, different reagents can be respectively added into six spaces of the liquid storage box I through the liquid storage box I, the liquid storage box I can be rotated to ensure that the corresponding air inlet groove is communicated with the connecting pipe, at the moment, the air pump I can be started, the air pump I can inject air into the corresponding space in the liquid storage box I through the connecting pipe and the corresponding air inlet groove, so that the corresponding reagent is pressed out of the liquid, the method is favorable for the experiment;
2. there is the suction tube through two top surfaces of stock solution box intercommunication, two inlet ends of air pump and two top surfaces of stock solution box intercommunication, air pump two can aspirate the air in the stock solution box two, can aspirate stock solution box two in with the reagent in the experiment box through the suction tube like this, do not need the manual suction of experimenter, and the operation is simple, and convenient to use, through placing the equal fixedly connected with cylinder in four turnings of box inner bottom surface, cylinder output end fixedly connected with and the lifter plate of experiment box bottom surface contact, when aspirating reagent, the cylinder can make experiment box rebound through the lifter plate, thereby make the suction tube bottom be located the square bottom of experiment box, the suction reagent of the suction tube of being convenient for, after the experiment is ended simultaneously, can remove the experiment box and place the box, be convenient for take out the experiment box, be favorable to going on of experiment.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic diagram of the overall structure of an apoptosis induction detection device and a method for using the same according to the present invention;
FIG. 2 is a schematic view of the support module of the present invention;
FIG. 3 is a schematic view of the structure of the placing case of the present invention;
FIG. 4 is a schematic structural view of a liquid feeding module in the present invention;
FIG. 5 is a schematic view of the inner structure of the baffle frame of the present invention;
FIG. 6 is a schematic view of the attachment ring structure of the present invention;
FIG. 7 is a schematic view of a positioning block according to the present invention;
FIG. 8 is a schematic view of an internal structure of the reservoir of the present invention;
FIG. 9 is a top cross-sectional view of the reservoir of the present invention;
FIG. 10 is a schematic view of a structure of a circular plate according to the present invention;
FIG. 11 is a schematic structural view of a circular plate II according to the present invention;
fig. 12 is a front view of a suction module in the present invention.
In the figure: 100. a support module; 110. a base plate; 120. a side lever; 130. a slide bar; 131. a T-shaped groove; 132. a T-shaped block; 140. an experimental box; 150. placing the box; 151. a cylinder; 152. a lifting plate; 200. a liquid feeding module; 210. a support mechanism; 211. a blocking frame; 2111. a first support frame; 212. a top plate; 213. a fixed mount; 214. a limiting block; 215. a spring; 216. fixing grooves; 220. a first liquid storage box; 221. a connecting ring; 222. positioning blocks; 223. fixing the rod; 224. a partition plate; 225. a circular plate I; 230. a first air pump; 240. a liquid delivery pipe; 250. a second circular plate; 251. a connecting pipe; 252. a connecting rod; 300. a suction module; 310. a second liquid storage box; 311. a second support frame; 320. a second air pump; 330. and (4) sucking the pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-12, a detecting device for apoptosis induction includes a supporting module 100, the supporting module 100 is fixedly connected with a liquid feeding module 200 for delivering a reagent and a suction module 300 for sucking waste liquid, the supporting module 100 includes a first liquid storage box 220, a connecting ring 221 is fixedly connected to a top surface of the first liquid storage box 220, a supporting mechanism 210 fixedly connected to the supporting module 100 is disposed on a side surface of the connecting ring 221, the supporting mechanism 210 can support the first liquid storage box 220 through the connecting ring 221, a fixing rod 223 is fixedly connected to a central position of an inner side surface of the first liquid storage box 220, six partition plates 224 fixedly connected to the inner side surface of the first liquid storage box 220 are fixedly connected to side surfaces of the fixing rod 223 in an annular and equidistant manner, the partition plates 224 can separate six spaces from the first liquid storage box 220, so as to facilitate storing different reagents, liquid feeding tubes 240 are fixedly connected between two adjacent partition, the round groove has been seted up to a 220 top surface central point of stock solution box department, a round groove medial surface fixedly connected with plectane 225, the air inlet duct has all been seted up between the plectane 225 top surface corresponds two baffles 224, plectane 225 top surface is provided with rotates the plectane two 250 of being connected with the round groove, the plectane two 250 top surfaces correspond the air inlet duct intercommunication that is located absorption module 300 one side has connecting pipe 251, supporting mechanism 210 fixedly connected with air pump 230, air pump 230 gives vent to anger the end and communicates with connecting pipe 251, air pump 230 can press the air into stock solution box 220 through connecting pipe 251 and air inlet duct, can extrude stock solution box 220 through liquid delivery pipe 240 with corresponding reagent like this, carry out the filling of reagent, do not need frequent manual absorption of experimenter to annotate reagent like this, and is easy to operate, be favorable to accelerating.
The supporting mechanism 210 comprises a blocking frame 211, two sides of the outer side of the blocking frame 211 are fixedly connected with a first supporting frame 2111 fixedly connected with the top surfaces of adjacent side rods 120, the first supporting frame 2111 can support the blocking frame 211, the top surface of the blocking frame 211 is fixedly connected with a top plate 212, the inner side surface of the top plate 212 is rotatably connected with the outer side surface of a connecting ring 221, the connecting ring 221 can support the first liquid storage box 220, the top surface of the top plate 212 is fixedly connected with a fixing frame 213, the top surface of the fixing frame 213 is fixedly connected with a base of a first air pump 230, the top surface of the fixing frame 213 is fixedly connected with a connecting pipe 251, the central position of the top surface of a second circular plate 250 is fixedly connected with the inner bottom surface of the fixing frame 213, the connecting rod 252 can limit the position of the second circular plate 250, so that the second circular plate 250 can not rotate along with the first liquid storage box 220, different reagents were filled.
Six locating pieces 222 that go up ring 221 top annular lateral surface annular equidistance fixed connection be connected with and roof 212 top surface contact, the spacing groove has been seted up to locating piece 222 bottom surface, fixed slot 216 has been seted up to six locating piece 222 annular equidistance that roof 212 top surface corresponds, fixed slot 216 has been seted up to fixed slot 216 medial surface sliding connection has with adjacent spacing groove sliding connection's stopper 214, stopper 214 bottom surface fixed connection has and fixed slot 216 fixed connection's spring 215, can rotate go up ring 221 through locating piece 222, thereby rotate liquid storage box 220, under spring 215's effect, can make stopper 214 block into the spacing inslot of next locating piece 222, carry out spacing fixed to locating piece 222, be convenient for correspond next air inlet duct and liquid feeding groove. The top surface of the circular plate II 250 is provided with a liquid adding groove corresponding to one air inlet groove, the inner side surface of the liquid adding groove is provided with a first blocking block, the first blocking block can be opened, and reagents are added into the first liquid storage box 220 through the liquid adding groove and the air inlet groove. The annular equidistance fixedly connected with of a 220 lateral wall of stock solution box and the fixed block of adjacent liquid sending pipe 240 lateral wall fixed connection, the fixed block can support liquid sending pipe 240.
The suction module 300 comprises a second liquid storage box 310, both sides of the bottom of the outer side of the second liquid storage box 310 are fixedly connected with a second support frame 311 fixedly connected with the top surface of the adjacent side rod 120, the top surface of the second liquid storage box 310 is communicated with a suction pipe 330, the top surface of the second liquid storage box 310 is fixedly connected with a second air pump 320, the air inlet end of the second air pump 320 is communicated with the top surface of the second liquid storage box 310, the placing box 150 can be moved to the bottom of the suction pipe 330, so that corresponding squares of the experiment box 140 correspond to the suction pipe 330, then the air cylinder 151 is started, the air cylinder 151 can move the experiment box 140 upwards through the lifting plate 152, so that the bottom end of the suction pipe 330 is positioned at the bottom of the squares of the experiment box 140, then the second air pump 320 can be started, the second air pump 320 can suck reagents into the second liquid storage box 310 through the suction pipe 330, waste, the inner side surface of the liquid discharge groove is provided with a second blocking block, and the second blocking block can be opened to discharge the waste liquid in the second liquid storage box 310.
A use method of a cell apoptosis induction detection device comprises the following specific use operation steps:
the method comprises the following steps: opening the first blocking block, filling reagents into the first liquid storage box 220 through the liquid adding groove and the air inlet groove, then rotating the connecting ring 221 through the positioning block 222 to enable the first liquid storage box 220 to rotate, so that other air inlet grooves correspond to the liquid adding grooves, and adding different reagents into different spaces in the first liquid storage box 220;
step two: after reagent adding is finished, the placing box 150 can be moved, so that squares of different experiment boxes 140 correspond to the bottoms of the liquid sending pipes 240 on one sides of the connecting pipes 251, then the air pump I230 is started, the air pump I230 can press reagents corresponding to the spaces in the liquid storage boxes I220 into the squares of the experiment boxes 140 through the liquid sending pipes 240, reagent adding is carried out, the liquid storage boxes I220 can be rotated, and reagents in other spaces in the liquid storage boxes I220 are added into the squares of the experiment boxes 140 through the air pump I230;
step three: the placing box 150 can be moved to the bottom of the suction pipe 330, the air cylinder 151 is started, the air cylinder 151 can enable the experiment box 140 to move upwards, so that the bottom end of the suction pipe 330 is positioned at the bottom of the square of the experiment box 140, then the air pump II 320 can be started, the air pump II 320 can suck the reagent into the liquid storage box II 310 through the suction pipe 330, and other squares of the experiment box 140 can be aligned to the suction pipe 330 to suck the reagent;
step four: after all the reagents are sucked and discharged from the grids, other reagents can be continuously added through the liquid feeding module 200, and after the experiment is finished, the air cylinder 151 can be started to push the experiment box 140 out of the placing box 150.
Can set up gravity sensor in the position department that the top surface of roof 212 corresponds the bottom surface of go-between 221 top, like this when filling reagent, can measure the whole weight of a stock solution box 220, subtract the whole weight of a stock solution box 220 after the filling with the whole weight of a stock solution box 220 before the filling, can learn the volume of filling reagent, be convenient for control the volume of filling reagent, and convenient to use, can set up power unit simultaneously on bottom plate 110, drive slide bar 130 and remove along side lever 120, the box 150 is placed in the drive simultaneously and removes along slide bar 130, do not need the experimenter to manually move like this and place box 150, be favorable to increasing the speed of experiment.
The working principle is as follows: when the device is used, the first blocking block is opened, reagents are filled into the first liquid storage box 220 through the liquid adding groove and the air inlet groove, then the connecting ring 221 is rotated through the positioning block 222, the first liquid storage box 220 is rotated, other air inlet grooves correspond to the liquid adding grooves, different reagents are added into different spaces in the first liquid storage box 220, and when the positioning block 222 is rotated, the limiting block 214 can be clamped into the limiting groove of the next positioning block 222 under the action of the spring 215 to limit and fix the positioning block 222, so that the next air inlet groove corresponds to the liquid adding groove;
after reagent adding is finished, the placing box 150 can be moved, so that squares of different experiment boxes 140 correspond to the bottoms of the liquid feeding pipes 240 on one sides of the connecting pipes 251, then the air pump I230 is started, the air pump I230 can press air into corresponding spaces in the liquid storage boxes I220, then reagents corresponding to the spaces in the liquid storage boxes I220 are pressed into the squares of the experiment boxes 140 through the liquid feeding pipes 240, the liquid storage boxes I220 can be rotated through the positioning blocks 222, other air inlet grooves are communicated with the connecting pipes 251, and therefore reagents in other spaces in the liquid storage boxes I220 can be added into the squares of the experiment boxes 140 through the air pump I230;
when reagent needs to be sucked, the box 150 can be placed and the bottom of the sucking pipe 330 is moved, corresponding grids of the experiment box 140 correspond to the sucking pipe 330, then the cylinder 151 is started, the cylinder 151 can make the experiment box 140 move upwards through the lifting plate 152, thereby the bottom end of the sucking pipe 330 is positioned at the bottom of the grids of the experiment box 140, then the air pump 320 can be started, the air pump 320 can suck the reagent into the liquid storage box 310 through the sucking pipe 330, then the experiment box 140 can be moved downwards, the sucking pipe 330 is aligned to other grids of the experiment box 140, reagent extraction is continuously performed, after all reagent suction and discharge grids, other reagents can be continuously added through the liquid feeding module 200, after the experiment is finished, the cylinder 151 can be started, the box 150 is placed by pushing the experiment box 140.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.
Claims (9)
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| CN202110495530.8A CN113122428B (en) | 2021-05-07 | 2021-05-07 | A kind of cell apoptosis induction detection device and using method thereof |
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| CN202110495530.8A CN113122428B (en) | 2021-05-07 | 2021-05-07 | A kind of cell apoptosis induction detection device and using method thereof |
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