CN113088523B - A kind of transposon and its application - Google Patents

Abstract

The invention discloses a transposon and its application. The LINE/L1 transposon provided by the present invention is any of the following: 1) the nucleotide sequence is sequence 5 in the sequence table; 2) hybridizes with the DNA sequence defined in 1) under stringent conditions and has the same encoding DNA molecules with functional amino acid residues; 3) and 1) defined DNA sequences with at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96% %, at least 97%, at least 98%, or at least 99% homologous and encoding DNA molecules with the same functional amino acid residues. The present invention finds the LINE/L1 transposon in the ZmCCT gene for the first time, and finds that the allele ZmCCT-FO containing the transposon can be used to identify the flowering time of the maize to be tested, and can also be used to identify the maize to be tested. plant height and ear height.

Description

A kind of transposon and its application

technical field

The invention belongs to the field of biotechnology, and in particular relates to a transposon and its application.

Background technique

Corn is one of the most important food crops in my country. In 2019, corn production was close to 40% of my country's total grain output. Maize maturity is one of the key traits affecting maize yield. Prolonged maize maturity, that is, later flowering of maize, will lead to a higher proportion of energy consumption in the vegetative growth stage and increase the possibility of field encounters with pests and diseases. In North China, prolonged corn maturity will also affect the rotation of the next crop. In Northeast China, prolonged corn maturity will increase the risk of frost damage and reduce yields. In addition, maize is domesticated from the tropical plant ruminant grass, which is sensitive to photoperiod and cannot bloom normally under high-latitude long-day conditions. Many tropical maize inbred lines are still sensitive to photoperiod and bloom in high-latitude temperate regions. The period is very late, or even does not bloom, which directly restricts the excellent alleles in tropical maize germplasm resources from being excavated and utilized.

SUMMARY OF THE INVENTION

An object of the present invention is to provide a LINE/L1 transposon.

The LINE/L1 transposon provided by the invention is any of the following:

1) The nucleotide sequence is sequence 5 in the sequence listing;

2) DNA molecules that hybridize under stringent conditions to the DNA sequences defined in 1);

3) The DNA sequences defined in 1) have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least DNA molecules with 98% or at least 99% homology.

The ZmCCT allele containing the above-mentioned LINE/L1 transposon is also within the scope of the present invention.

The above-mentioned ZmCCT allele is ZmCCT-FO, which is any of the following:

1) The nucleotide sequence is sequence 14 in the sequence listing;

2) Hybridize with the DNA sequence defined in 1) under stringent conditions and encode a DNA molecule with the same functional amino acid residue;

3) The DNA sequences defined in 1) have at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least DNA molecules having 98% or at least 99% homology and encoding amino acid residues with the same function.

The application of the following substances of A or B in identifying at least one of the following 1)-3) is also the scope of protection of the present invention:

A. The material shown is a material for detecting whether the ZmCCT gene in the maize genome to be tested contains the LINE/L1 transposon;

B, the material shown is to detect that the ZmCCT gene in the corn genome to be tested is the material of allele ZmCCT-FO (sequence 15), allele ZmCCT-F7 or allele ZmCCT-OGD;

1) Identify the flowering time of the corn to be tested sooner or later;

2) Identify the plant height of the corn to be tested;

3) Identify the ear height of the corn to be tested.

In the above application, the material for detecting whether the ZmCCT gene in the maize genome to be tested contains LINE/L1 transposon is a primer for amplifying the LINE/L1 transposon; the primer is specifically composed of an IIA primer and an IIB primer;

The IIA primer is composed of the single-stranded DNA molecule shown in Sequence 6 in the Sequence Listing and the single-stranded DNA molecule described in Sequence 7 in the Sequence Listing;

Or, the IIA primer is composed of the single-stranded DNA molecule shown in Sequence 10 in the Sequence Listing and the single-stranded DNA molecule described in Sequence 11 in the Sequence Listing;

The IIB primer is composed of the single-stranded DNA molecule shown in Sequence 8 in the Sequence Listing and the single-stranded DNA molecule described in Sequence 9 in the Sequence Listing;

Or, the IIB primer consists of the single-stranded DNA molecule shown in SEQ ID NO: 12 in the Sequence Listing and the single-stranded DNA molecule described in SEQ ID NO: 13 in the Sequence Listing.

The substance A or substance B in the above application is also within the protection scope of the present invention.

Described to detect whether the ZmCCT gene in the maize genome to be tested is allele ZmCCT-FO (sequence 14), allele ZmCCT-F7 (nucleotide sequence is sequence 1) or allele ZmCCT-OGD (nucleotide sequence 1) The substances of sequence 2) are as follows:

The homozygous insertion of the 5.1-kb transposon was identified using primers M7-1 and M7-2 in Table 1. The primer M7-1 amplified no band, and the individual plant with a band amplified by M7-2 was 5.1 -Kb transposon homozygous insertion into a single plant; identify 4.2-kb LINE/L1 transposon homozygous insertion using IIA and IIB in Table 2, primer IIA amplified with bands, and IIB amplified without bands The individual plant is a LINE/L1 transposon homozygous insertion plant.

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and a 4.2-kb LINE/L1 transposon homozygous insertion in the intron region has the ZmCCT allele ZmCCT-FO.

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and no 4.2-kb LINE/L1 transposon insertion in the intron region has the ZmCCT allele ZmCCT-OGD.

A single plant without a 5.1-kb transposon insertion in the promoter region of the ZmCCT gene and a homozygous insertion of a 4.2-kb LINE/L1 transposon in the intron region has the ZmCCT allele ZmCCT-F7.

Still another object of the present invention is to provide the following method.

The present invention provides a method for identifying the flowering time of corn to be tested. In order to detect whether the ZmCCT gene in the genome of the corn to be tested contains the above-mentioned LINE/L1 transposon, the ZmCCT gene in the genome contains the LINE/L1 transposon. The flowering time of the tested maize is earlier than or the candidate is earlier than the tested maize that does not contain the LINE/L1 transposon in the ZmCCT gene in the genome;

Or, a method for identifying the flowering time of corn to be tested sooner or later provided by the present invention is to detect whether the ZmCCT gene in the genome of corn to be tested is allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD , the flowering time of the tested maize with allele ZmCCT-FO is earlier or candidate earlier than the tested maize with allele ZmCCT-F7 or with allele ZmCCT-OGD.

Or a method for identifying the plant height of maize to be tested provided by the present invention is to detect whether the ZmCCT gene in the maize genome to be tested contains the above-mentioned LINE/L1 transposon, and the ZmCCT gene in the genome contains the LINE/L1 transposon The tested maize plant height is lower than or the candidate is lower than the ZmCCT gene in the genome to be tested without the LINE/L1 transposon;

Or, a method for identifying the plant height of maize to be tested provided by the present invention is to detect whether the ZmCCT gene in the maize genome to be tested is allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD, The plant height of the tested maize with the allele ZmCCT-FO is lower or candidate lower than that of the tested maize with the allele ZmCCT-F7 or with the allele ZmCCT-OGD.

Or, a method for identifying the ear height of maize to be tested provided by the present invention is to detect whether the ZmCCT gene in the maize genome to be tested contains the above-mentioned LINE/L1 transposon, and the ZmCCT gene in the genome contains the LINE/L1 transposon. The corn ear height of the transposon to be tested is lower than or the candidate is lower than the ZmCCT gene in the genome of the corn to be tested that does not contain the LINE/L1 transposon;

Or, a method for identifying the ear height of maize to be tested provided by the present invention is to detect whether the ZmCCT gene in the maize genome to be tested is allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD , the ear height of the tested maize with the allele ZmCCT-FO is lower or the candidate is lower than the tested maize with the allele ZmCCT-F7 or with the allele ZmCCT-OGD.

Whether the above-mentioned ZmCCT gene in the maize genome to be tested contains the above-mentioned LINE/L1 transposon is as follows:

Use primers IIA and IIB to amplify the maize genome to be tested, respectively, and judge according to the following methods:

If primer IIA has a band in amplification, and IIB has no band in amplification, it means that the ZmCCT genes of the two chromosomes in the maize genome to be tested both contain LINE/L1 transposon and are homozygous for LINE/L1 transposon;

If the primer IIB has a band, and the IIA has no band, it means that the ZmCCT gene of the two chromosomes in the maize genome to be tested does not contain the LINE/L1 transposon, and it is homozygous for the deletion of the LINE/L1 transposon. type;

If both primer IIA and primer IIB have bands, it means that the ZmCCT gene of one chromosome in the tested maize genome has LINE/L1 transposon insertion, while the other chromosome does not have LINE/L1 transposon insertion, which is LINE/ L1 transposon heterozygous.

The aforementioned primer IIA is primer IIA1 or primer IIA2, and the aforementioned primer IIB is primer IIB1 or primer IIB2.

The size of the target amplified fragment of the IIA1 primer was 493 bp, and the size of the target amplified fragment of the IIB1 primer was 1007 bp.

The size of the target amplified fragment of the IIA2 primer is 591 bp, and the size of the target amplified fragment of the IIB2 primer is 1588 bp.

The above-mentioned method for detecting whether the ZmCCT gene in the maize genome to be tested is the allele ZmCCT-FO, the allele ZmCCT-F7 or the allele ZmCCT-OGD is as follows:

It can be detected by sequencing or by the following methods:

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and a 4.2-kb LINE/L1 transposon homozygous insertion in the intron region has the ZmCCT allele ZmCCT-FO.

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and no 4.2-kb LINE/L1 transposon insertion in the intron region has the ZmCCT allele ZmCCT-OGD.

A single plant without a 5.1-kb transposon insertion in the promoter region of the ZmCCT gene and a homozygous insertion of a 4.2-kb LINE/L1 transposon in the intron region has the ZmCCT allele ZmCCT-F7.

The above-identified 5.1-kb transposon insertion used primers M7-1 and M7-2 in Table 1, primer M7-1 amplified no band, and M7-2 amplified a single plant with a band of 5.1- The kb transposon was homozygous for insertion into the individual plant.

The above-identified LINE/L1 transposon insertion of 4.2-kb adopts IIA and IIB in Table 2, the primer IIA amplified with a band, and the individual plant with no band amplified by IIB was a LINE/L1 transposon homozygous insertion single plant.

The present invention finds the LINE/L1 transposon in the ZmCCT gene for the first time, and finds the allele ZmCCT-FO containing the transposon, and the transposon and the allele can be used to identify the flowering time of the maize to be tested sooner or later, It can also be used to identify the plant height and ear height of the corn to be tested.

Description of drawings

Figure 1 shows the results of QTL mapping at the flowering stage of maize chromosome 10.

Figure 2 shows the results of fine mapping of QTLs at the flowering stage of No. 10.

Figure 3 shows the relative expression levels of all 7 genes in the 520-kb interval.

Figure 4 is a PCR amplification of the ZmCCT gene introns of the parents.

Figure 5 is an analysis of the variation sites of ZmCCT gene between parents.

Fig. 6 shows the pollination stage, plant height, ear height and number of leaves on ear of NILs-F7 and NILs-OGD.

Figure 7 shows the rhythm and accumulation of ZmCCT gene expression in NILs-F7 and NILs-OGD.

Figure 8 is a PCR amplification of parental ZmCCT transcripts.

Figure 9 shows the ZmCCT expression levels and flowering phenotypes of ZmCCT-FO, ZmCCT-OGD and ZmCCT-F7.

Detailed ways

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

The very early maize inbred line F7 was provided by the Germplasm Resource Information Network (GRIN) with the family number PI 257507.

Corn landrace OGD is a commercial variety, full name Oaxacan Green Dent, purchased online (https://www.anniesheirloomseeds.com/).

The remaining heterozygous family (HIF) of the F ₇ generation is the F ₁ generation obtained from the cross between F7 and OGD, and the F ₁ generation is obtained by self-crossing for 6 generations. The ZmCCT gene remains heterozygous (M2 in Table 1 is used for identification, M2 is located on the ZmCCT gene, M2 amplification to obtain 2 heterozygous bands represents the F _{7 generation individual plant with the ZmCCT gene remaining heterozygous), and the family composed of all the progeny obtained from the self-crossing of the F 7 generation} individual plant.

Embodiment 1. Discovery of transposon and its application in ZmCCT near-isogenic line

1. Discovery of LINE/L1 transposon

1. By crossing, a very early-maturing maize inbred line F7 from Europe and an F ₂ population of OGD (OAXACAN GREEN DENT), a corn landrace of normal maturity from Mexico, were assembled. QTL mapping of the F ₂ population in the low-latitude Hainan, a major QTL affecting the flowering period of maize was located near the 94-Mb chromosome of chromosome 10, which could explain 15.3% of the phenotypic variation, with an additive effect of 1.6 days (Fig. 1).

2. Using 5800 individuals from 19 remaining heterozygous families (HIFs) for fine mapping, this QTL was narrowed down to a 520-kb interval by screening for new crossover individuals and molecular markers, located at markers M4 and M7 between (Figure 2, Table 1).

Table 1 is the primer sequence

This interval contains 7 genes. By detecting the expression levels of these 7 genes in leaves at V3 stage in two near-isogenic lines (NILs), it is found that ZmCCT is the only one with significant difference in expression (P<0.01). genes (Figure 3, Table 1).

ZmCCT has been reported to regulate the flowering stage of maize, and the difference in ZmCCT expression between parental NILs is consistent with the early and late flowering phenotypes, so ZmCCT was identified as a candidate gene (Figure 6).

3. According to the B73 reference genome, ZmCCT contains two exons and one intron, encoding a 240-aa protein, containing a CO CO-LIKE TIMING OF CAB1 (CCT) domain, the domain from 196-aa to 237-aa (XP_008662996, commit date January 10, 2020).

4. DNA sequencing was performed on the parental material, and it was found that the ZmCCT gene of the parent OGD (the nucleotide sequence was sequence 2, the allele was named ZmCCT-OGD, and the amino acid sequence of the encoded protein was sequence 4) was 2561-bp in length. There is a known 5.1-kb transposon insertion in the promoter region, and the length of the ZmCCT gene of parent F7 (nucleotide sequence is sequence 1, allele is named ZmCCT-F7, and the amino acid sequence of the encoded protein is sequence 3) is 6730-bp, at position 2292-6461, in the first intron only 26-bp away from the second exon, there is an unreported LINE/L1 transposon insertion, transposon length 4170 -bp (Figure 4).

In addition, within the coding region, there are 5 SNPs between ZmCCT-F7 and ZmCCT-OGD, 3 of which lead to amino acid substitutions (SEQ ID NO: 3, SEQ ID NO: 4). In the NAM (Nested Association Mapping) population, the ZmCCT sequence of the common parent B73 is consistent with that of F7, and the ZmCCT sequences of the parents B97, M37W and MS71 are consistent with those of OGD, but the subpopulations in which B73 and these three parents were assembled respectively are in ZmCCT No flowering QTLs above the threshold were detected nearby (Fig. 5), indicating that these five SNPs are not functional loci.

In summary, it is speculated that the LINE/L1 transposon insertion is the only functional site.

After sequencing, the nucleotide sequence of the LINE/L1 transposon (also known as the 4.2-kb LINE/L1 transposon) was sequence 5.

2. Relationship between LINE/L1 transposon and flowering time

1. By selfing in a heterozygous HIF line of F ₇ generation, two NILs of F ₈ generation were obtained. The dispersing stage of NILs-F7 in low-latitude Hainan was 6.0 days earlier than that of NILs-OGD (P<0.01) ( Image 6). Further study of the expression pattern of ZmCCT in these two NILs under short-day conditions found that ZmCCT-F7 had a clear rhythmic expression, while ZmCCT-OGD had no expression rhythm, and the expression level at each time point exceeded that of ZmCCT-F7. , so that the accumulation of ZmCCT expression in one day was also significantly higher than that of ZmCCT-F7 (P<0.01) (Fig. 7).

In addition, PCR detection and sequencing of the cDNAs of the two NILs revealed that this transposon insertion did not generate new transcripts (Fig. 8, Table 1). Based on this, it is inferred that the insertion of the LINE/L1 transposon in the intron region of the parental F7 inhibited the expression of ZmCCT, thereby advancing the flowering period, and making the NILs containing the ZmCCT-F7 allele show a precocious phenotype. In addition, NILs-F7 and NILs-OGD had significant differences in several plant morphological traits. Compared with NILs-OGD, the plant height and ear height of NILs-F7 decreased by 22 and 18 cm, respectively, and the number of leaves on the ear decreased by 1.1 slices (P<0.01) (Fig. 6).

Therefore, it is possible to judge whether the ZmCCT gene in the genome of the maize to be tested has LINE/L1 transposon to determine whether the flowering time of the maize to be tested is sooner or later, and the flowering time of the maize containing the LINE/L1 transposon is earlier than that without the LINE/L1 transposon. the corn of the seat;

Or, by detecting whether the ZmCCT gene in the genome of the maize to be tested has a LINE/L1 transposon, the plant height of the maize to be tested is judged, and the plant height of the maize containing the LINE/L1 transposon is lower than that of the maize that does not contain the LINE/L1 transposon. corn;

Or, by detecting whether the ZmCCT gene in the maize genome to be tested has a LINE/L1 transposon, the ear height of the maize to be tested is judged, and the ear height of the maize containing the LINE/L1 transposon is lower than that without the LINE/L1 transposon. of corn.

3. Acquisition and application of ZmCCT-FO gene

Perform 5.1-kb transposon insertion detection and 4.2-kb LINE/L1 transposon insertion detection on the F ₄ to F ₇ generation individual plants in the above-mentioned one;

5.1-kb transposon insertion detection using primers M7-1 and M7-2 in Table 1, primer M7-1 amplified no band, and M7-2 amplified a single plant with a band of 5.1-kb homozygous insertion of the transposon into a single plant.

4. The 2-kb LINE/L1 transposon uses IIA and IIB in Table 2, the primer IIA amplified with a band, and the IIB amplified the individual plant without the band is the LINE/L1 transposon homozygous insertion individual plant.

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and a 4.2-kb LINE/L1 transposon homozygous insertion in the intron region was selected. The ZmCCT allele was named as ZmCCT-FO, the nucleotide sequence of the ZmCCT-FO allele is sequence 14 (position 2292-6461 of sequence 14 is the LINE/L1 transposon).

A single plant with a 5.1-kb transposon homozygous insertion in the ZmCCT gene promoter region and no 4.2-kb LINE/L1 transposon insertion in the intron region was selected, and the ZmCCT allele it had was ZmCCT-OGD ( sequence 2).

A single plant with no 5.1-kb transposon insertion in the promoter region of the ZmCCT gene and a 4.2-kb LINE/L1 transposon homozygous insertion in the intron region was selected, and the ZmCCT allele it had was ZmCCT-F7 ( Sequence 1, wherein positions 2292-6461 are LINE/L1 transposon).

The individual plants with ZmCCT-FO alleles, the individual plants with ZmCCT-OGD alleles, and the individual plants with ZmCCT-F7 alleles in the high-latitude long-day Beijing area were subjected to flowering stage detection and real-time fluorescence quantification PCR detection (primers are ZmCCT in Table 1).

The results are shown in Figure 9. It can be seen that compared with the other two alleles, ZmCCT-F7 and ZmCCT-OGD, ZmCCT-FO has the lowest expression level of ZmCCT in the high-latitude and long-day Beijing area, the earliest flowering period, and mature the shortest period (Figure 9).

Therefore, it is also possible to detect whether the ZmCCT gene in the genome is the ZmCCT-FO allele, the ZmCCT-F7 allele or the ZmCCT-OGD allele to identify the flowering time of the maize to be tested: the waiting time containing the allele ZmCCT-FO The flowering time of the tested maize was earlier or candidate earlier than that of the tested maize containing alleles ZmCCT-F0 and ZmCCT-OGD.

The above meets the following judgment criteria: the flowering time of the tested maize whose ZmCCT gene in the genome contains the LINE/L1 transposon is earlier or the candidate is earlier than the tested maize whose ZmCCT gene in the genome does not contain the LINE/L1 transposon.

4. Establishment of a method for identifying the flowering time, plant height or ear height of LINE/L1 transposon or ZmCCT-FO allele, ZmCCT-F7 allele or ZmCCT-OGD allele

A. Application of LINE/L1 transposon

1. Extract the genomic DNA of the corn to be tested

Extract genomic DNA from the maize leaf tissue to be tested.

2. PCR identification of transposons

According to the upstream and downstream sequences of the LINE/L1 transposon, design primers IIA and IIB as shown in Table 2 below, where IIA is IIA1 or IIA2, and IIB is IIB1 or IIB2;

Table 2 shows the sequences of primers IIA and IIB used to identify transposons

Using the genomic DNA obtained in the above 1 as a template, PCR amplification was performed on IIA1/IIB1 with the primer pairs shown in Table 2, respectively, to obtain PCR amplification products.

The reaction system of above-mentioned PCR amplification is as shown in Table 3 below:

Table 3 is the reaction system of PCR amplification

Component Volume 10×PCR Buffer(with MgCl₂) 1μL dNTP (2.5mM) 0.2μL Upstream primer (10uM) 0.2μL Downstream primer (10μM) 0.2μL Taq polymerase (5U/μL) 0.2μL ddH₂O 7.2μL DNA 1μL total 10μL

The above-mentioned PCR amplification reaction program is shown in Table 4 below:

Table 4 is the reaction program of PCR amplification

*Note: Primer IIA uses 30s extension time, IIB uses 1min extension time

The size of the amplified product was detected by agarose gel electrophoresis.

If primer IIA has a band in amplification, and IIB has no band in amplification, it means that the ZmCCT genes of the two chromosomes in the maize genome to be tested both contain LINE/L1 transposon and are homozygous for LINE/L1 transposon;

If the primer IIB has a band, and the IIA has no band, it means that the ZmCCT gene of the two chromosomes in the maize genome to be tested does not contain the LINE/L1 transposon, and it is homozygous for the deletion of the LINE/L1 transposon. type;

If both primer IIA and primer IIB have bands, it means that the ZmCCT gene of one chromosome in the tested maize genome has LINE/L1 transposon insertion, while the other chromosome has no LINE/L1 transposon insertion, which is LINE/ L1 transposon heterozygous.

The aforementioned primer IIA is primer IIA1 or primer IIA2, and the aforementioned primer IIB is primer IIB1 or primer IIB2.

The size of the target amplified fragment of the IIA1 primer was 493 bp, and the size of the target amplified fragment of the IIB1 primer was 1007 bp.

The size of the target amplified fragment of the IIA2 primer is 591 bp, and the size of the target amplified fragment of the IIB2 primer is 1588 bp.

The flowering time of the tested maize whose ZmCCT gene in the genome contains LINE/L1 transposon is earlier or the candidate is earlier than the tested maize whose ZmCCT gene in the genome does not contain LINE/L1 transposon;

Or, the ZmCCT gene in the genome contains LINE/L1 transposon to be tested maize plant height is lower than or candidate is lower than the ZmCCT gene in the genome does not contain LINE/L1 transposon to be tested maize;

Or, the maize to be tested whose ZmCCT gene in the genome contains LINE/L1 transposon is lower than or candidate is lower than the maize to be tested whose ZmCCT gene in the genome does not contain LINE/L1 transposon.

B. to detect

1. Extract the genomic DNA of the corn to be tested

Extract genomic DNA from the maize leaf tissue to be tested.

2. Detect whether the ZmCCT gene in the corn to be tested is the ZmCCT-FO allele, the ZmCCT-F7 allele or the ZmCCT-OGD allele

It can be detected by sequencing or by the following methods:

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and a 4.2-kb LINE/L1 transposon homozygous insertion in the intron region has the ZmCCT allele ZmCCT-FO.

A single plant with a 5.1-kb transposon homozygous insertion in the promoter region of the ZmCCT gene and no 4.2-kb LINE/L1 transposon insertion in the intron region has the ZmCCT allele ZmCCT-OGD.

A single plant without a 5.1-kb transposon insertion in the promoter region of the ZmCCT gene and a homozygous insertion of a 4.2-kb LINE/L1 transposon in the intron region has the ZmCCT allele ZmCCT-F7.

The above-identified 5.1-kb transposon insertion used primers M7-1 and M7-2 in Table 1, primer M7-1 amplified no band, and M7-2 amplified a single plant with a band of 5.1- The kb transposon was homozygous for insertion into the individual plant.

The above-identified LINE/L1 transposon insertion of 4.2-kb adopts IIA and IIB in Table 2, the primer IIA amplified with a band, and the individual plant with no band amplified by IIB was a LINE/L1 transposon homozygous insertion single plant.

The flowering time of the tested maize containing the allele ZmCCT-FO was earlier or candidate earlier than that of the tested maize containing the allele ZmCCT-F0 or ZmCCT-OGD.

Or, the plant height of the maize to be tested containing the allele ZmCCT-FO is lower than or candidate is lower than the maize to be tested containing the allele ZmCCT-F0 or the maize to be tested containing the allele ZmCCT-OGD.

Or, the height of the corn to be tested containing the allele ZmCCT-FO is lower than or candidate is lower than the corn to be tested containing the allele ZmCCT-F0 or the corn to be tested containing the allele ZmCCT-OGD.

Embodiment 2, the application of LINE/L1 transposon in identifying the flowering time, plant height and ear position of corn to be tested sooner or later

1. Extract the genomic DNA of the corn to be tested

Extract genomic DNA from the maize leaf tissue to be tested.

The maize to be tested is each family in Table 5, and each family has 22, 32, 28, 36, 44, 32, 72, 63, 69 and 147 strains respectively:

1) ft1-1*, ft1-2*, ft2-1*, ft2-2*, ft3-1*, ft3-2* are prepared as follows:

The parents are F7 and OGD, the two parents are crossed to obtain F ₁ generation, F ₁ is self-crossed for 7 generations, and F ₇ is obtained; from F ₇ , select the F ₇ generation individual plant with the ZmCCT gene remaining heterozygous (using M2 in Table 1 to identify , M2 is located on the ZmCCT gene, and M2 amplifies and obtains 2 heterozygous bands on behalf of the ZmCCT gene to maintain heterozygosity), the family composed of all progeny obtained by selfing of these F ₇ generations of individual plants, ft1-1* and ft1-2*, ft2-1* and ft2-2*, ft3-1* and ft3-2* from 3 different F ₇ generation individual plants, respectively.

2) ft4-1** and ft 4-2** were prepared as follows:

The parents are F7 and OGD. The two parents were crossed to obtain the F ₁ generation, and the F ₁ self-crossed for 4 generations to obtain the F ₅ generation; from the F ₅ generation, the promoter region of the ZmCCT gene was selected to have a known pure 5.1-kb transposon. Co-insertion (primers are M7-1 and M7-2 in Table 1, primer M7-1 amplifies no band, and M7-2 amplifies a single strain with a known 5.1-kb transposon Homozygous inserted individual), and the LINE/L1 transposon insertion remained heterozygous individual (primers were IIA and IIB in Table 2, primer IIA amplified a band, and IIB amplified a band with a single clone). The 4.2-kb LINE/L1 transposon was inserted into the individual plant to maintain heterozygosity), the family composed of all the progeny obtained by selfing of these F ₅ generation individual plants, ft4-1** and ft4-2 **From the same F _5th generation single plant;

3) ft5-1*** and ft5-2*** are prepared as follows:

The parents are F7 and OGD, the two parents were crossed to obtain F ₁ generation, F ₁ was selfed for 6 generations, and F ₇ generation was obtained; the promoter region of ZmCCT gene was selected from the F ₇ generation with a known pure 5.1-kb transposon. The inserted strains (primers are M7-1 and M7-2 in Table 1, primer M7-1 amplifies no band, and M7-2 amplifies a single strain with a known 5.1-kb homozygous insertion of the transposon).

2. PCR identification of transposons

The method is the same as A of Example 1.

The flowering time of the tested maize whose ZmCCT gene in the genome contains the LINE/L1 transposon is earlier or the candidate is earlier than the tested maize whose ZmCCT gene in the genome does not contain the LINE/L1 transposon.

The ZmCCT gene in the genome contains the LINE/L1 transposon to be tested, the plant height is lower or the candidate is lower than the tested maize that the ZmCCT gene in the genome does not contain the LINE/L1 transposon.

The ZmCCT gene in the genome contains the LINE/L1 transposon to be tested, and the ear height of the tested maize is lower or the candidate is lower than the ZmCCT gene in the genome does not contain the LINE/L1 transposon to be tested.

The results are shown in Table 5. Among them, the -1 lines are all the individual plants that lack the LINE/L1 transposon insertion, and the -2 lines are all the individual plants that have the LINE/L1 transposon homozygous insertion; It can be seen that the flowering time of all the individual plants with homozygous insertion of the LINE/L1 transposon is earlier than that of all the individual plants with the deletion of the LINE/L1 transposon insertion, and the plant height of all the individual plants with the homozygous insertion of the LINE/L1 transposon The height of ear position and ear position were lower than all the individual plants with the deletion of LINE/L1 transposon insertion, and the -1 line and the corresponding -2 line were significantly different.

Table 5 shows the identification results

SEQUENCE LISTING

<110> China Agricultural University

<120> A kind of transposon and its application

<160> 14

<170> PatentIn version 3.5

<210> 1

<211> 6730

<212> DNA

<213> Artificial sequence

<400> 1

atgtcgtcgg ggccagcagc atgcggtgtg tgcggcgcgg ccgcctgctg cccgcacctc 60

ttgcacaccg gtgacggcaa cgacgacgac ctcatcagcc gggccttctt ctccgtcttc 120

cctgtcgtcg gtcatcaccg tcgtcatgag tccaccagca gccccgccat gcagcagcca 180

tcggggtgcc tgcacgagtt ccagttcttt ggccatcagg acgaccacca ccaccaagaa 240

accatcgcct ggctcttgga ccacccaccg ccacctgcgc ccgagcttgg cggcgacgac 300

ggcccgtccc cagctggtga tgagaacgac gaccagcctg cgtttcaccc gtttgggaca 360

ccacagtacc accaccccgg aaaagggaac gggaacgggc tcacctttga gctggacgcc 420

acgctgggcc tcggcaccgc gcggcaaacc actgagacag cagaagcaag cgccaccatc 480

gtaagtattg ctcccgaatt atcttaagta agttcagata attcacatgc atggtttcta 540

attggaattt ggtcccaagc tggacaccct ttttttatct tccgttttct caactctctt 600

atcgatcacc tgcataaagg acctttgtat caagtaccaa gagatcttgc catgagttgc 660

actttacgca cattttttttt tctttttttt tttcaggaac gtactactct tcctatatat 720

caatatatgt aaacaagatt aacatgcatg tttctaacct ttctcaaaga caaaagacac 780

tctggtgcac gaaatggatg gaagaaacca gatcattaat atatgcctca caacctcttc 840

atgaatttaa tttgatgtgg aaagaataaa aacgacggtt ccggttgtta acccaatatt 900

caatgatatc ctgaacaaaa ctagctatag atctcaatca tagcatcagg catcagcgct 960

tccaaagttc tcacctgact ttttttttac tcaatctcca gattatattt ccttcctaca 1020

aagagtcgga gagaacatag ccatgagtta aatcactgat gttgtaaata cagaccagta 1080

gtcaaaagca ttgactatac actaaaacta ttgttcaagg tcactatttc acaaaaaaat 1140

tcattgccta tttgatagtt tgattgagag gtagcaatat tgccaaattt atttttcacg 1200

tacctagaca aaagtcggta gcaatattgc caattttatt gctccatcgt catatgcatc 1260

ccgaagtcta ttattgctgt aatgacaaga tacagatctt ttatattgtg atatacttac 1320

ttaagtttta tattgaagat aaaagggaga aagcagcttg cctccctttc tttttcttca 1380

ccactatata tattggattg tttcttcacc actatatata caagaaaata ttaatatctg 1440

cagtacatat ttagtgtcat taaatatgtc ttttgaaact attttcataa taaacatatt 1500

tgaagataca tgtattgcaa atatttttta cgaatctaat caaatatgag aaattttgac 1560

tgacatgtat gaccatacta tcaattattt taggacagag gcatggagtg cgcatttgta 1620

tggtcgaaat cgatcaattg taaccatata tgcatgtacg tttggtacgc ccactgatgt 1680

atctacctgg ttaattaatt agatgaccta gcttgtcgtc tgattgttat gattaaagaa 1740

ccaaaaagtc tactcagctc aaaacccaaa tatatatgtg tcaaacaact cccatgcaca 1800

tgtccagctg tgtctaaatc tatcccgaag gattgtccat gccaaagttt gatgaaatag 1860

ataataagtt gtctcatttt atgtggttcg tttttgcaga tttgctgctt actctttcgt 1920

atacttggat tttataggga actaatatat acatatgatt ataattaatg cactttattc 1980

cgtgccacat gtagatgaat aacgcaatca catggcttaa gatctaatat tctaccccaa 2040

aacaaatcga gctaccaagg cgatatctga tgttcatcag gcatgcatgt aggcccattc 2100

agcatatcaa gcaaagtaca gattcttatc caaaccatgc atatacatat gaccaaagta 2160

ctaattaatt agttgcctgc agttattagc tgtccaaaat ttgctttgat catcatgcaa 2220

taatatacac atgcagaaac taaaatgaat aacatatata aatccatgca tgcacatgca 2280

gcatactttt tttttgaaaa ctggcaagag aattgcctgt tatattaaaa agaaggtgag 2340

agccagcgag ggctaaaata tacaagtaca tcacactagg gaatgagaca ttaagtatgg 2400

tgacacaaat gttccaaaac caacccctct ccttccccaa aacctacacc agccttgcta 2460

agtgttttgc tcctcctaga atccacaggc gaatttgctc cttaatcatc gctatagtct 2520

gtgtcacggt cttctctttg tgctcaaaga ttcgcgagtt tctctcacac caaattgtcc 2580

aagacacgag gatgaggagg gttcgcaatc ccttcacgct tctcactgcg gatgttgcaa 2640

gattcgacca ccattggtgc accgaatcta caggttgcca agaagaatgg tggatctccg 2700

taattgcagc ccaaatactc aaatcagccc aaatatgtct ggtgaagcaa cattcagcaa 2760

aaagatgtag tccagattct gggcatgagg cgcataggac acagcgcgga ttatgaggcc 2820

atccccttct tgccaacctg tctgctgtcc aaactctatt ttgaacagca agccaactga 2880

agaatttgca ctttggtggc gcccaagctt tccaaattat actattaaaa ttcctgcatg 2940

tggagccata aaattgcgct tggtaggctg actgagttgt gtactggcca ctcatagtaa 3000

acttccactt gataaggtcg ggggtattgt gcattaagcg aagctgattt acctcgcacc 3060

ataattgaca atattcatgt atgtgcgcgg cagaaaaatc attgtgatta aaatgtaggt 3120

cctgaatcca tcggttgttt gagagagcct catggatgga tctatttttg ccgttagaaa 3180

tggagtaggt caaagggaag gtgtctctag gtgtcgtacc ccttagccag gcactttccc 3240

agaacaaaga agacttacca tcccccaccc tgactgaggt tgctgctgca aacagtctcc 3300

tgtccaacat attgcaaggt atatctttcg ttatcctctg agtatttaac agtttggcct 3360

cctgccacaa ccatctgagg cgtaaggccc gcgagaaaaa accaaggtgc agaatgccta 3420

aaccaccgta ttgttttggt cttgctgcac agacccagtt gactttgcat ttaccgccgg 3480

ttaatctctc tgaaccagcc caaagaaatt gcttacgttt tgagtcaatg aaatccaaca 3540

ccctttgggg cggttttagg gctaggagca gataggtgac ctgcgaggtt agcactgcat 3600

tgactagcgt gaggcggcca gccgccgaaa ggttttttccc gctccatgta ctcagctttg 3660

cagagacttt gtcaatccat ggctggaagt ccacccgttt gagtctgttc atggacaaag 3720

gtagtccaag gtatttaagg gggaaggaag tcctcgaggc cggtagacca gatagaacgt 3780

cagagaggtt gatgttgtta cactggattg gcactactgt ggacttgtgg aaatttgttt 3840

ttaggcctgt ggtttcacca aaaagttcca gaatccttgc aagcatagat acctcacctt 3900

ttgtgggcgt gacaaatatg accgcatcat ccgcaaacat cgatatgcgc aggtcgggcg 3960

agcgaccatg gagcttggtg agcattccga attcagttgc tacttcgagg agtctttgca 4020

ggggatctat tgcaatgaca aaaagaagag gagataggggg gtcgccttgc cttaggcctc 4080

gcccgtgcca gatagggggg tttggtacgc cgttaaggat gactcttgag gttgaggtgg 4140

agagaattgc cgcaatccat tcccgccacc ttatagggaa gcctaggtgc tcaaggaggg 4200

tgaggatata ctcccaacgt atcgaatcaa aggcttttgc tatgtccaat ttgaacaaga 4260

gtgttggggt cttgtttgta tgaaaacgac gtgccgcggt acgcactgcc aagaagttat 4320

catgtatgct cctgttcttg atgaaggcgc tttgacaagt ggagacgatc gcgttcatat 4380

ggggctgaag tcgtaatgcc agtatcttgg aaataagctt tatgaaggaa tgtattaagc 4440

ttatcggtct aaaatcaccc acttcttcgg ccccttcttt ttttgggatt aggattacat 4500

tagcggtgtt gatgagtgat aagctgccac aacgtagagc atggaaagcg ttggccgccc 4560

ttatgacatc cccttttatt atgctccagc acgtcttaaa aaataaccca gtgaaaccat 4620

caggtcccgg cgccttgtca atgggcaaca gatcaatggc tcttttgatc tcctcctctg 4680

agaatggagc agctaaagaa gagagatcat ggtgtcgtaa tccaagcgtc gcccagttga 4740

agtctattct cggagcgggt ggacgactca acatattttc aaagtgagat tgtattttcg 4800

cggccttgca ttcatgtgtt gttgtcgagc cattttggtc tttgaggcaa tgaatgaaat 4860

tttttcgacg cctagaagtg atccttcgat gaaaaaatct agtgttagca tccccaaatt 4920

ttatcaaatt tagacgtgca gcctgttttt tccgcgcacg ttcgatgacc gcaaggccca 4980

gaattctttt cttaagtctg catcttagga gctcctctcc aggagagaga gctctcagct 5040

cctgtgctat gtcaaatcta tggattatct ccaaagccat atgaagttgc agcttagcat 5100

ccgagatagt gttgaagctc cactgtctga gggcccttgc tgtagcctgc agtttgtgat 5160

agagtctgtg gaaaggctcc tggtgtgtgc agtgagcgca ccaggacctc gacaccactt 5220

ccatgaatcc tgggagcatg gcccaaaaat tctcaaattt aaaagagcgc gggcgtcggg 5280

ggccagtttg gttagagagc agcagtggac agtgatccga gagcgaagaa gataggccgt 5340

gcagcacgtg gctatgaaaa gcttggtccc attcagcatt tgcaaagacc ctgtcaagct 5400

taataagggt tgggtttgta cgctcgttgc tccaagtgaa tcgtctattt tgcaggttaa 5460

tttccttcag gtcacaacag tctagcatat cactgaaacg gctcattagg ctaaggttca 5520

gacgcctctt attcttgtca ctagctttgt aaattagatt aaaatctccc aaaagcagcc 5580

acttaattcc ggattgcggt ttcagatctt gaatttcttg gagaaaggct gtcttcatgc 5640

tattgcttgt aggcccataa accacagtta ataggaaggc agtatgggac gctgttagct 5700

tggcctttcc agaaatatgg aaatcaccaa ctgtaaaatc agtcagctcc acatggttgg 5760

tatcccatag caatgcaatc cctccccgtg tgccgctggg gcctccagcc ggtttgcaga 5820

agaatttgtc taggtggtgg cctccaaggt gccaggctgt tgtttggtcg aaggaagaca 5880

atttagtttc ttgcaaacaa gctaagtgac acctcgatga agtaattgtc tctctgaccg 5940

tgtccttccg agcctgggaa ttgagacccc tcacattcca gcaaaaaacc ttaaggtcta 6000

agtctgtcat tgggaaaaaa aggaggtacc ccttgaatca tagtttcctg ctctctcagc 6060

acagtgacaa gacagtgtgc atggaaacca catatctggt gaatgaggtg cgtcgagaca 6120

ttgatggtgc agggaatgca aaaggaccat acaagaggtg ttcacgccat acatcaaaca 6180

catgcagtct gaagcctccg cacacaagcg aggcaggaat tcaaagccta aagttaacat 6240

gatagaagca aaagaccctt aagcacagat cactggctca tgctgctgac tgcagctcct 6300

caacgcgcag actcctgatg ccatcttgca agtctgcaac tccgtccccc atcatcgcaa 6360

tcatagcatc ctcgaagctc ttctctgtcg acccttcgag gttgaaggcg gacgttaggg 6420

ctgcaagtgt ctcctgtggc agagtccccg tggtcgtgta ctaattattg ctattaatta 6480

attgcagatg tcattctctg ggagcacatt cacggacgct gcaagcaagg agccagcact 6540

gatcgacgac ggcaatgagc tgcaaatgcc ggtagatcag tcgtcgacgg agagggaggt 6600

taagttgatg aggtacaagg agaagaggat gaggaggtgc tttgagaagc agataagata 6660

tgcatccagg aaagcctatg cgcaggtgag acccagggtg aaaggccgct ttgccaaggt 6720

aaccgaatga 6730

<210> 2

<211> 2561

<212> DNA

<213> Artificial sequence

<400> 2

atgtcgtcgg ggccagcagc atgcggtgtg tgcggcgcgg ccgcctgctg cccgcacctc 60

ttgcacaccg gtgacggcaa cgacgacgac ctcatcagcc gggccttctt ctccgtcttc 120

cctgtcgtcg gtcatcaccg tcgtcatgag tccaccagca gccccgccat gcagcagcca 180

tcggggtgcc tgcacgagtt ccagttcttt ggccaccagg acgaccacca ccaccaagaa 240

accatcgcct ggctcttgga ccacccaccg ccacctgcgc ccgagcttgg cggcgacgac 300

ggcccgtccc tagctggtga tgagaacgac gaccagcctg cgtttcaccc gtttgggaca 360

ccacagtacc accaccccgg aaaagggaac gggaacgggc tcacctttga gctggacgcc 420

acgctgggcc tcggcaccgc gcggcaaacc actgagacag cagaagcaag cgccaccatc 480

gtaagtattg ctcccgaatt atcttaagta agttcagata attcacatgc atggtttcta 540

attggaattt ggtcccaagc tggacaccct ttttttatct tccgttttct caactctctt 600

atcgatcacc tgcataaagg acctttgtat caagtaccaa gagatcttgc catgagttgc 660

actttacgca cattttttttt ttcttttttt tttcaggaac gtactactct tcctatatat 720

caatatatgt aaacaagatt aacatgcatg tttctaacct ttctcaaaga caaaagacac 780

tctggtgcac gaaatggatg gaagaaacca gatcattaat atatgcctca caacctcttc 840

atgaatttaa tttgatgtgg aaagaataaa aacgacggtt ccggttatta acccaatatt 900

caatgatatc ctgaacaaaa ctagctatag atctcaatca tagcatcagg catcagcgct 960

tccaaagttc tcacctgact ttttttttac tcaatctcca gattatattt ccttcctaca 1020

aagagtcgga gagaacatag ccatgagtta aatcactgat gttgtaaata cagaccagta 1080

gtcaaaagca ttgactatac actaaaacta ttgttcaagg tcactatttc acaaaaaaat 1140

tcattgccta tttgatagtt tgattgagag gtagcaatat tgccaaattt atttttcacg 1200

tatctagaca aaatcggtag caatattgcc aattttattg ctccatcgtc atatccagcc 1260

cgaagtctat tgctgtaatg acaagataca tatatatctt ttatattgtg atatacttac 1320

ttaagttata tattgacgat aaaagggaga aagcagcttg cctccctttc tttcttcacc 1380

actatacata ttggattgtt tcttcaccac aatatacaag aaaatattaa tatctgcagt 1440

acatatttgg tgtcattaaa tatgtctttt gaaactattt tcataataaa catatttgaa 1500

gatacagata tttcaaatat tttttacgaa tctaatcaaa tatgagaaat tttgactgac 1560

atgtatgacc atactatcaa ttattttagg acagaggcag ggagtgcgca tttgtatggt 1620

cgaaatcgat caattgtaac catatgcgtg tacgttggta cgcccactga tgtatctacc 1680

tggttaatta attagatata tgacctagct tgtcgtctga ttgttatgat taaagaacca 1740

aaaagtctac tcagctcaaa acccaaatat atatgtgtca aacaactccc atgcacatgt 1800

ccagctgtga ctaaatctat cccgaaggat tgtccatgcc aaagtttgat gaaataggta 1860

ataagttgtc tcattttatg tggttcattt gcagatttgc tgcttactct ttggtatact 1920

tggattttat agggaactaa tatatacata tgattataat taatgcactt tattccgtgc 1980

cacatgtaga cgatgaacaa ctcaatcaca tggcttaaga tctaactaat attctaccag 2040

cccaaacaaa tggtgctacc aaggcaatat ctgatgttca tcaggcatgc atgtagctag 2100

gcccattcag catatcaagc aaagtacaga ttcttatcca acaaacaata tatatgatca 2160

aagtactaat tgattagttg cctgcagtta gctgtccaaa atttgctttg atcatcatgc 2220

aataatatac acatgcagaa actaaaatgc aataacatat ataaatccat gcatgcatgc 2280

agcatatata catactaatt gctattaatt aattgcagat gtcattctgt gggagcacat 2340

tcactgacgc tgcaagcaag gagccagcac tgatcgacga cggcaatgag ctgcaaatgc 2400

cggtagatca gtcgtcgtcg gagagggagg ttaagttgat gaggtacaag gagaagagga 2460

tgaggaggtg ctttgagaag cagataagat atgcatccag gaaagcctat gcgcaggtga 2520

gacccagggt gaaaggccgc tttgccaagg taaccgaatg a 2561

<210> 3

<211> 240

<212> PRT

<213> Artificial sequence

<400> 3

Met Ser Ser Gly Pro Ala Ala Cys Gly Val Cys Gly Ala Ala Ala Cys

1 5 10 15

Cys Pro His Leu Leu His Thr Gly Asp Gly Asn Asp Asp Asp Leu Ile

20 25 30

Ser Arg Ala Phe Phe Ser Val Phe Pro Val Val Gly His His Arg Arg

35 40 45

His Glu Ser Thr Ser Ser Pro Ala Met Gln Gln Pro Ser Gly Cys Leu

50 55 60

His Glu Phe Gln Phe Phe Gly His Gln Asp Asp His His His Gln Glu

65 70 75 80

Thr Ile Ala Trp Leu Leu Asp His Pro Pro Pro Pro Ala Pro Glu Leu

85 90 95

Gly Gly Asp Asp Gly Pro Ser Pro Ala Gly Asp Glu Asn Asp Asp Gln

100 105 110

Pro Ala Phe His Pro Phe Gly Thr Pro Gln Tyr His His Pro Gly Lys

115 120 125

Gly Asn Gly Asn Gly Leu Thr Phe Glu Leu Asp Ala Thr Leu Gly Leu

130 135 140

Gly Thr Ala Arg Gln Thr Thr Glu Thr Ala Glu Ala Ser Ala Thr Ile

145 150 155 160

Met Ser Phe Ser Gly Ser Thr Phe Thr Asp Ala Ala Ser Lys Glu Pro

165 170 175

Ala Leu Ile Asp Asp Gly Asn Glu Leu Gln Met Pro Val Asp Gln Ser

180 185 190

Ser Thr Glu Arg Glu Val Lys Leu Met Arg Tyr Lys Glu Lys Arg Met

195 200 205

Arg Arg Cys Phe Glu Lys Gln Ile Arg Tyr Ala Ser Arg Lys Ala Tyr

210 215 220

Ala Gln Val Arg Pro Arg Val Lys Gly Arg Phe Ala Lys Val Thr Glu

225 230 235 240

<210> 4

<211> 240

<212> PRT

<213> Artificial sequence

<400> 4

Met Ser Ser Gly Pro Ala Ala Cys Gly Val Cys Gly Ala Ala Ala Cys

1 5 10 15

Cys Pro His Leu Leu His Thr Gly Asp Gly Asn Asp Asp Asp Leu Ile

20 25 30

Ser Arg Ala Phe Phe Ser Val Phe Pro Val Val Gly His His Arg Arg

35 40 45

His Glu Ser Thr Ser Ser Pro Ala Met Gln Gln Pro Ser Gly Cys Leu

50 55 60

His Glu Phe Gln Phe Phe Gly His Gln Asp Asp His His His Gln Glu

65 70 75 80

Thr Ile Ala Trp Leu Leu Asp His Pro Pro Pro Pro Ala Pro Glu Leu

85 90 95

Gly Gly Asp Asp Gly Pro Ser Leu Ala Gly Asp Glu Asn Asp Asp Gln

100 105 110

Pro Ala Phe His Pro Phe Gly Thr Pro Gln Tyr His His Pro Gly Lys

115 120 125

Gly Asn Gly Asn Gly Leu Thr Phe Glu Leu Asp Ala Thr Leu Gly Leu

130 135 140

Gly Thr Ala Arg Gln Thr Thr Glu Thr Ala Glu Ala Ser Ala Thr Ile

145 150 155 160

Met Ser Phe Cys Gly Ser Thr Phe Thr Asp Ala Ala Ser Lys Glu Pro

165 170 175

Ala Leu Ile Asp Asp Gly Asn Glu Leu Gln Met Pro Val Asp Gln Ser

180 185 190

Ser Ser Glu Arg Glu Val Lys Leu Met Arg Tyr Lys Glu Lys Arg Met

195 200 205

Arg Arg Cys Phe Glu Lys Gln Ile Arg Tyr Ala Ser Arg Lys Ala Tyr

210 215 220

Ala Gln Val Arg Pro Arg Val Lys Gly Arg Phe Ala Lys Val Thr Glu

225 230 235 240

<210> 5

<211> 4170

<212> DNA

<213> Artificial sequence

<400> 5

ttttgaaaac tggcaagaga attgcctgtt atattaaaaa gaaggtgaga gccagcgagg 60

gctaaaatat acaagtacat cacactaggg aatgagacat taagtatggt gacacaaatg 120

ttccaaaacc aacccctctc cttccccaaa acctacacca gccttgctaa gtgttttgct 180

cctcctagaa tccacaggcg aatttgctcc ttaatcatcg ctatagtctg tgtcacggtc 240

ttctctttgt gctcaaagat tcgcgagttt ctctcacacc aaattgtcca agacacgagg 300

atgaggaggg ttcgcaatcc cttcacgctt ctcactgcgg atgttgcaag attcgaccac 360

cattggtgca ccgaatctac aggttgccaa gaagaatggt ggatctccgt aattgcagcc 420

caaatactca aatcagccca aatatgtctg gtgaagcaac attcagcaaa aagatgtagt 480

ccagattctg ggcatgaggc gcataggaca cagcgcggat tatgaggcca tccccttctt 540

gccaacctgt ctgctgtcca aactctattt tgaacagcaa gccaactgaa gaatttgcac 600

tttggtggcg cccaagcttt ccaaattata ctattaaaat tcctgcatgt ggagccataa 660

aattgcgctt ggtaggctga ctgagttgtg tactggccac tcatagtaaa cttccacttg 720

ataaggtcgg gggtattgtg cattaagcga agctgattta cctcgcacca taattgacaa 780

tattcatgta tgtgcgcggc agaaaaatca ttgtgattaa aatgtaggtc ctgaatccat 840

cggttgtttg agagagcctc atggatggat ctatttttgc cgttagaaat ggagtaggtc 900

aaagggaagg tgtctctagg tgtcgtaccc cttagccagg cactttccca gaacaaagaa 960

gacttaccat cccccaccct gactgaggtt gctgctgcaa acagtctcct gtccaacata 1020

ttgcaaggta tatctttcgt tatcctctga gtatttaaca gtttggcctc ctgccacaac 1080

catctgaggc gtaaggcccg cgagaaaaaa ccaaggtgca gaatgcctaa accaccgtat 1140

tgttttggtc ttgctgcaca gacccagttg actttgcatt taccgccggt taatctctct 1200

gaaccagccc aaagaaattg cttacgtttt gagtcaatga aatccaacac cctttggggc 1260

ggttttaggg ctaggagcag ataggtgacc tgcgaggtta gcactgcatt gactagcgtg 1320

aggcggccag ccgccgaaag gtttttcccg ctccatgtac tcagctttgc agagactttg 1380

tcaatccatg gctggaagtc cacccgtttg agtctgttca tggacaaagg tagtccaagg 1440

tatttaaggg ggaaggaagt cctcgaggcc ggtagaccag atagaacgtc agagaggttg 1500

atgttgttac actggattgg cactactgtg gacttgtgga aatttgtttt taggcctgtg 1560

gtttcaccaa aaagttccag aatccttgca agcatagata cctcaccttt tgtgggcgtg 1620

acaaatatga ccgcatcatc cgcaaacatc gatatgcgca ggtcgggcga gcgaccatgg 1680

agcttggtga gcattccgaa ttcagttgct acttcgagga gtctttgcag gggatctatt 1740

gcaatgacaa aaagaagagg agataggggg tcgccttgcc ttaggcctcg cccgtgccag 1800

ataggggggt ttggtacgcc gttaaggatg actcttgagg ttgaggtgga gagaattgcc 1860

gcaatccatt cccgccacct tatagggaag cctaggtgct caaggagggt gaggatatac 1920

tcccaacgta tcgaatcaaa ggcttttgct atgtccaatt tgaacaagag tgttggggtc 1980

ttgtttgtat gaaaacgacg tgccgcggta cgcactgcca agaagttatc atgtatgctc 2040

ctgttcttga tgaaggcgct ttgacaagtg gagacgatcg cgttcatatg gggctgaagt 2100

cgtaatgcca gtatcttgga aataagcttt atgaaggaat gtattaagct tatcggtcta 2160

aaatcaccca cttcttcggc cccttctttt tttgggatta ggattacatt agcggtgttg 2220

atgagtgata agctgccaca acgtagagca tggaaagcgt tggccgccct tatgacatcc 2280

ccttttatta tgctccagca cgtcttaaaa aataacccag tgaaaccatc aggtcccggc 2340

gccttgtcaa tgggcaacag atcaatggct cttttgatct cctcctctga gaatggagca 2400

gctaaagaag agagatcatg gtgtcgtaat ccaagcgtcg cccagttgaa gtctattctc 2460

ggagcgggtg gacgactcaa catattttca aagtgagatt gtattttcgc ggccttgcat 2520

tcatgtgttg ttgtcgagcc attttggtct ttgaggcaat gaatgaaatt ttttcgacgc 2580

ctagaagtga tccttcgatg aaaaaatcta gtgttagcat ccccaaattt tatcaaattt 2640

agacgtgcag cctgttttttt ccgcgcacgt tcgatgaccg caaggcccag aattcttttc 2700

ttaagtctgc atcttaggag ctcctctcca ggagagagag ctctcagctc ctgtgctatg 2760

tcaaatctat ggattatctc caaagccata tgaagttgca gcttagcatc cgagatagtg 2820

ttgaagctcc actgtctgag ggcccttgct gtagcctgca gtttgtgata gagtctgtgg 2880

aaaggctcct ggtgtgtgca gtgagcgcac caggacctcg acaccacttc catgaatcct 2940

gggagcatgg cccaaaaatt ctcaaattta aaagagcgcg ggcgtcgggg gccagttttgg 3000

ttagagagca gcagtggaca gtgatccgag agcgaagaag ataggccgtg cagcacgtgg 3060

ctatgaaaag cttggtccca ttcagcattt gcaaagaccc tgtcaagctt aataagggtt 3120

gggtttgtac gctcgttgct ccaagtgaat cgtctatttt gcaggttaat ttccttcagg 3180

tcacaacagt ctagcatatc actgaaacgg ctcattaggc taaggttcag acgcctctta 3240

ttcttgtcac tagctttgta aattagatta aaatctccca aaagcagcca cttaattccg 3300

gattgcggtt tcagatcttg aatttcttgg agaaaggctg tcttcatgct attgcttgta 3360

ggcccataaa ccacagttaa taggaaggca gtatgggacg ctgttagctt ggcctttcca 3420

gaaatatgga aatcaccaac tgtaaaatca gtcagctcca catggttggt atcccatagc 3480

aatgcaatcc ctccccgtgt gccgctgggg cctccagccg gtttgcagaa gaatttgtct 3540

aggtggtggc ctccaaggtg ccaggctgtt gtttggtcga aggaagacaa tttagtttct 3600

tgcaaacaag ctaagtgaca cctcgatgaa gtaattgtct ctctgaccgt gtccttccga 3660

gcctgggaat tgagacccct cacattccag caaaaaacct taaggtctaa gtctgtcatt 3720

gggaaaaaaa ggaggtaccc cttgaatcat agtttcctgc tctctcagca cagtgacaag 3780

acagtgtgca tggaaaccac atatctggtg aatgaggtgc gtcgagacat tgatggtgca 3840

gggaatgcaa aaggaccata caagaggtgt tcacgccata catcaaacac atgcagtctg 3900

aagcctccgc acacaagcga ggcaggaatt caaagcctaa agttaacatg atagaagcaa 3960

aagaccctta agcacagatc actggctcat gctgctgact gcagctcctc aacgcgcaga 4020

ctcctgatgc catcttgcaa gtctgcaact ccgtccccca tcatcgcaat catagcatcc 4080

tcgaagctct tctctgtcga cccttcgagg ttgaaggcgg acgttagggc tgcaagtgtc 4140

tcctgtggca gagtccccgt ggtcgtgtac 4170

<210> 6

<211> 24

<212> DNA

<213> Artificial sequence

<400> 6

cccttgaatc atagtttcct gctc 24

<210> 7

<211> 23

<212> DNA

<213> Artificial sequence

<400> 7

tgaatgtgct cccagagaat gac 23

<210> 8

<211> 25

<212> DNA

<213> Artificial sequence

<400> 8

attgaagata aaagggagaa agcag 25

<210> 9

<211> 22

<212> DNA

<213> Artificial sequence

<400> 9

gtgaatgtgc tcccagagaa tg 22

<210> 10

<211> 24

<212> DNA

<213> Artificial sequence

<400> 10

atacaagagg tgttcacgcc atac 24

<210> 11

<211> 21

<212> DNA

<213> Artificial sequence

<400> 11

caggcttcgt cattcggtta c 21

<210> 12

<211> 24

<212> DNA

<213> Artificial sequence

<400> 12

gatgtggaaa gaataaaaac gacg 24

<210> 13

<211> 25

<212> DNA

<213> Artificial sequence

<400> 13

gtacctcatc aacttaacct ccctc 25

<210> 14

<211> 6730

<212> DNA

<213> Artificial sequence

<400> 14

atgtcgtcgg ggccagcagc atgcggtgtg tgcggcgcgg ccgcctgctg cccgcacctc 60

ttgcacaccg gtgacggcaa cgacgacgac ctcatcagcc gggccttctt ctccgtcttc 120

cctgtcgtcg gtcatcaccg tcgtcatgag tccaccagca gccccgccat gcagcagcca 180

tcggggtgcc tgcacgagtt ccagttcttt ggccaccagg acgaccacca ccaccaagaa 240

accatcgcct ggctcttgga ccacccaccg ccacctgcgc ccgagcttgg cggcgacgac 300

ggcccgtccc tagctggtga tgagaacgac gaccagcctg cgtttcaccc gtttgggaca 360

ccacagtacc accaccccgg aaaagggaac gggaacgggc tcacctttga gctggacgcc 420

acgctgggcc tcggcaccgc gcggcaaacc actgagacag cagaagcaag cgccaccatc 480

gtaagtattg ctcccgaatt atcttaagta agttcagata attcacatgc atggtttcta 540

attggaattt ggtcccaagc tggacaccct ttttttatct tccgttttct caactctctt 600

atcgatcacc tgcataaagg acctttgtat caagtaccaa gagatcttgc catgagttgc 660

actttacgca cattttttttt ttcttttttt tttcaggaac gtactactct tcctatatat 720

caatatatgt aaacaagatt aacatgcatg tttctaacct ttctcaaaga caaaagacac 780

tctggtgcac gaaatggatg gaagaaacca gatcattaat atatgcctca caacctcttc 840

atgaatttaa tttgatgtgg aaagaataaa aacgacggtt ccggttatta acccaatatt 900

caatgatatc ctgaacaaaa ctagctatag atctcaatca tagcatcagg catcagcgct 960

tccaaagttc tcacctgact ttttttttac tcaatctcca gattatattt ccttcctaca 1020

aagagtcgga gagaacatag ccatgagtta aatcactgat gttgtaaata cagaccagta 1080

gtcaaaagca ttgactatac actaaaacta ttgttcaagg tcactatttc acaaaaaaat 1140

tcattgccta tttgatagtt tgattgagag gtagcaatat tgccaaattt atttttcacg 1200

tacctagaca aaagtcggta gcaatattgc caattttatt gctccatcgt catatgcatc 1260

ccgaagtcta ttattgctgt aatgacaaga tacagatctt ttatattgtg atatacttac 1320

ttaagtttta tattgaagat aaaagggaga aagcagcttg cctccctttc tttttcttca 1380

ccactatata tattggattg tttcttcacc actatatata caagaaaata ttaatatctg 1440

cagtacatat ttagtgtcat taaatatgtc ttttgaaact attttcataa taaacatatt 1500

tgaagataca tgtattgcaa atatttttta cgaatctaat caaatatgag aaattttgac 1560

tgacatgtat gaccatacta tcaattattt taggacagag gcatggagtg cgcatttgta 1620

tggtcgaaat cgatcaattg taaccatata tgcatgtacg tttggtacgc ccactgatgt 1680

atctacctgg ttaattaatt agatgaccta gcttgtcgtc tgattgttat gattaaagaa 1740

ccaaaaagtc tactcagctc aaaacccaaa tatatatgtg tcaaacaact cccatgcaca 1800

tgtccagctg tgtctaaatc tatcccgaag gattgtccat gccaaagttt gatgaaatag 1860

ataataagtt gtctcatttt atgtggttcg tttttgcaga tttgctgctt actctttcgt 1920

atacttggat tttataggga actaatatat acatatgatt ataattaatg cactttattc 1980

cgtgccacat gtagatgaat aacgcaatca catggcttaa gatctaatat tctaccccaa 2040

aacaaatcga gctaccaagg cgatatctga tgttcatcag gcatgcatgt aggcccattc 2100

agcatatcaa gcaaagtaca gattcttatc caaaccatgc atatacatat gaccaaagta 2160

ctaattaatt agttgcctgc agttattagc tgtccaaaat ttgctttgat catcatgcaa 2220

taatatacac atgcagaaac taaaatgaat aacatatata aatccatgca tgcacatgca 2280

gcatactttt tttttgaaaa ctggcaagag aattgcctgt tatattaaaa agaaggtgag 2340

agccagcgag ggctaaaata tacaagtaca tcacactagg gaatgagaca ttaagtatgg 2400

tgacacaaat gttccaaaac caacccctct ccttccccaa aacctacacc agccttgcta 2460

agtgttttgc tcctcctaga atccacaggc gaatttgctc cttaatcatc gctatagtct 2520

gtgtcacggt cttctctttg tgctcaaaga ttcgcgagtt tctctcacac caaattgtcc 2580

aagacacgag gatgaggagg gttcgcaatc ccttcacgct tctcactgcg gatgttgcaa 2640

gattcgacca ccattggtgc accgaatcta caggttgcca agaagaatgg tggatctccg 2700

taattgcagc ccaaatactc aaatcagccc aaatatgtct ggtgaagcaa cattcagcaa 2760

aaagatgtag tccagattct gggcatgagg cgcataggac acagcgcgga ttatgaggcc 2820

atccccttct tgccaacctg tctgctgtcc aaactctatt ttgaacagca agccaactga 2880

agaatttgca ctttggtggc gcccaagctt tccaaattat actattaaaa ttcctgcatg 2940

tggagccata aaattgcgct tggtaggctg actgagttgt gtactggcca ctcatagtaa 3000

acttccactt gataaggtcg ggggtattgt gcattaagcg aagctgattt acctcgcacc 3060

ataattgaca atattcatgt atgtgcgcgg cagaaaaatc attgtgatta aaatgtaggt 3120

cctgaatcca tcggttgttt gagagagcct catggatgga tctatttttg ccgttagaaa 3180

tggagtaggt caaagggaag gtgtctctag gtgtcgtacc ccttagccag gcactttccc 3240

agaacaaaga agacttacca tcccccaccc tgactgaggt tgctgctgca aacagtctcc 3300

tgtccaacat attgcaaggt atatctttcg ttatcctctg agtatttaac agtttggcct 3360

cctgccacaa ccatctgagg cgtaaggccc gcgagaaaaa accaaggtgc agaatgccta 3420

aaccaccgta ttgttttggt cttgctgcac agacccagtt gactttgcat ttaccgccgg 3480

ttaatctctc tgaaccagcc caaagaaatt gcttacgttt tgagtcaatg aaatccaaca 3540

ccctttgggg cggttttagg gctaggagca gataggtgac ctgcgaggtt agcactgcat 3600

tgactagcgt gaggcggcca gccgccgaaa ggttttttccc gctccatgta ctcagctttg 3660

cagagacttt gtcaatccat ggctggaagt ccacccgttt gagtctgttc atggacaaag 3720

gtagtccaag gtatttaagg gggaaggaag tcctcgaggc cggtagacca gatagaacgt 3780

cagagaggtt gatgttgtta cactggattg gcactactgt ggacttgtgg aaatttgttt 3840

ttaggcctgt ggtttcacca aaaagttcca gaatccttgc aagcatagat acctcacctt 3900

ttgtgggcgt gacaaatatg accgcatcat ccgcaaacat cgatatgcgc aggtcgggcg 3960

agcgaccatg gagcttggtg agcattccga attcagttgc tacttcgagg agtctttgca 4020

ggggatctat tgcaatgaca aaaagaagag gagataggggg gtcgccttgc cttaggcctc 4080

gcccgtgcca gatagggggg tttggtacgc cgttaaggat gactcttgag gttgaggtgg 4140

agagaattgc cgcaatccat tcccgccacc ttatagggaa gcctaggtgc tcaaggaggg 4200

tgaggatata ctcccaacgt atcgaatcaa aggcttttgc tatgtccaat ttgaacaaga 4260

gtgttggggt cttgtttgta tgaaaacgac gtgccgcggt acgcactgcc aagaagttat 4320

catgtatgct cctgttcttg atgaaggcgc tttgacaagt ggagacgatc gcgttcatat 4380

ggggctgaag tcgtaatgcc agtatcttgg aaataagctt tatgaaggaa tgtattaagc 4440

ttatcggtct aaaatcaccc acttcttcgg ccccttcttt ttttgggatt aggattacat 4500

tagcggtgtt gatgagtgat aagctgccac aacgtagagc atggaaagcg ttggccgccc 4560

ttatgacatc cccttttatt atgctccagc acgtcttaaa aaataaccca gtgaaaccat 4620

caggtcccgg cgccttgtca atgggcaaca gatcaatggc tcttttgatc tcctcctctg 4680

agaatggagc agctaaagaa gagagatcat ggtgtcgtaa tccaagcgtc gcccagttga 4740

agtctattct cggagcgggt ggacgactca acatattttc aaagtgagat tgtattttcg 4800

cggccttgca ttcatgtgtt gttgtcgagc cattttggtc tttgaggcaa tgaatgaaat 4860

tttttcgacg cctagaagtg atccttcgat gaaaaaatct agtgttagca tccccaaatt 4920

ttatcaaatt tagacgtgca gcctgttttt tccgcgcacg ttcgatgacc gcaaggccca 4980

gaattctttt cttaagtctg catcttagga gctcctctcc aggagagaga gctctcagct 5040

cctgtgctat gtcaaatcta tggattatct ccaaagccat atgaagttgc agcttagcat 5100

ccgagatagt gttgaagctc cactgtctga gggcccttgc tgtagcctgc agtttgtgat 5160

agagtctgtg gaaaggctcc tggtgtgtgc agtgagcgca ccaggacctc gacaccactt 5220

ccatgaatcc tgggagcatg gcccaaaaat tctcaaattt aaaagagcgc gggcgtcggg 5280

ggccagtttg gttagagagc agcagtggac agtgatccga gagcgaagaa gataggccgt 5340

gcagcacgtg gctatgaaaa gcttggtccc attcagcatt tgcaaagacc ctgtcaagct 5400

taataagggt tgggtttgta cgctcgttgc tccaagtgaa tcgtctattt tgcaggttaa 5460

tttccttcag gtcacaacag tctagcatat cactgaaacg gctcattagg ctaaggttca 5520

gacgcctctt attcttgtca ctagctttgt aaattagatt aaaatctccc aaaagcagcc 5580

acttaattcc ggattgcggt ttcagatctt gaatttcttg gagaaaggct gtcttcatgc 5640

tattgcttgt aggcccataa accacagtta ataggaaggc agtatgggac gctgttagct 5700

tggcctttcc agaaatatgg aaatcaccaa ctgtaaaatc agtcagctcc acatggttgg 5760

tatcccatag caatgcaatc cctccccgtg tgccgctggg gcctccagcc ggtttgcaga 5820

agaatttgtc taggtggtgg cctccaaggt gccaggctgt tgtttggtcg aaggaagaca 5880

atttagtttc ttgcaaacaa gctaagtgac acctcgatga agtaattgtc tctctgaccg 5940

tgtccttccg agcctgggaa ttgagacccc tcacattcca gcaaaaaacc ttaaggtcta 6000

agtctgtcat tgggaaaaaa aggaggtacc ccttgaatca tagtttcctg ctctctcagc 6060

acagtgacaa gacagtgtgc atggaaacca catatctggt gaatgaggtg cgtcgagaca 6120

ttgatggtgc agggaatgca aaaggaccat acaagaggtg ttcacgccat acatcaaaca 6180

catgcagtct gaagcctccg cacacaagcg aggcaggaat tcaaagccta aagttaacat 6240

gatagaagca aaagaccctt aagcacagat cactggctca tgctgctgac tgcagctcct 6300

caacgcgcag actcctgatg ccatcttgca agtctgcaac tccgtccccc atcatcgcaa 6360

tcatagcatc ctcgaagctc ttctctgtcg acccttcgag gttgaaggcg gacgttaggg 6420

ctgcaagtgt ctcctgtggc agagtccccg tggtcgtgta ctaattattg ctattaatta 6480

attgcagatg tcattctctg ggagcacatt cacggacgct gcaagcaagg agccagcact 6540

gatcgacgac ggcaatgagc tgcaaatgcc ggtagatcag tcgtcgacgg agagggaggt 6600

taagttgatg aggtacaagg agaagaggat gaggaggtgc tttgagaagc agataagata 6660

tgcatccagg aaagcctatg cgcaggtgag acccagggtg aaaggccgct ttgccaaggt 6720

aaccgaatga 6730

Claims (6)

1. Use of the following substances of A or B in identifying at least one of the following 1)-3): A. The material shown is a material for detecting whether the ZmCCT gene in the maize genome to be tested contains LINE/L1 transposon; B, the material shown is to detect that the ZmCCT gene in the maize genome to be tested is the material of allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD ; The nucleotide sequence of the LINE/L1 transposon is sequence 5 in the sequence listing; The nucleotide sequence of the ZmCCT allele ZmCCT-FO is sequence 14; The nucleotide sequence of the ZmCCT allele ZmCCT-OGD is sequence 2; The nucleotide sequence of the ZmCCT allele ZmCCT-F7 is sequence 1; 1) Identify the flowering time of the corn to be tested sooner or later; 2) Identify the plant height of the corn to be tested; 3) Identify the ear height of the corn to be tested. 2 . The application according to claim 1 , wherein the substance for detecting whether the ZmCCT gene in the maize genome to be tested contains a LINE/L1 transposon is a primer for amplifying the LINE/L1 transposon. 3 . 3. application according to claim 2, is characterized in that: described primer comprises IIA primer and IIB primer; The IIA primer is composed of the single-stranded DNA molecule shown in Sequence 6 in the Sequence Listing and the single-stranded DNA molecule described in Sequence 7 in the Sequence Listing; Or the IIA primer is composed of the single-stranded DNA molecule shown in Sequence 10 in the Sequence Listing and the single-stranded DNA molecule described in Sequence 11 in the Sequence Listing; The IIB primer is composed of the single-stranded DNA molecule shown in Sequence 8 in the Sequence Listing and the single-stranded DNA molecule described in Sequence 9 in the Sequence Listing; Or, the IIB primer is composed of the single-stranded DNA molecule shown in Sequence 12 in the Sequence Listing and the single-stranded DNA molecule shown in Sequence 13 in the Sequence Listing. 4. A method for identifying the flowering time of maize to be tested sooner or later, for detecting that the ZmCCT gene in the maize genome to be tested is allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD , and has an allelic The flowering time of the tested maize of the gene ZmCCT-FO is earlier than or the candidate is earlier than the tested maize with the allele ZmCCT-F7 or with the allele ZmCCT-OGD ; The nucleotide sequence of the ZmCCT allele ZmCCT-FO is sequence 14; The nucleotide sequence of the ZmCCT allele ZmCCT-OGD is sequence 2; The nucleotide sequence of the ZmCCT allele ZmCCT-F7 is sequence 1. 5. a method for identifying the plant height of maize to be tested, for detecting that the ZmCCT gene in the maize genome to be tested is allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD , with allele The tested maize plant height of ZmCCT-FO is lower than or candidate is lower than the tested maize with allele ZmCCT-F7 or with allele ZmCCT-OGD ; The nucleotide sequence of the ZmCCT allele ZmCCT-FO is sequence 14; The nucleotide sequence of the ZmCCT allele ZmCCT-OGD is sequence 2; The nucleotide sequence of the ZmCCT allele ZmCCT-F7 is sequence 1. 6. A method for identifying the flowering time of maize to be tested sooner or later, for detecting that the ZmCCT gene in the maize genome to be tested is allele ZmCCT-FO, allele ZmCCT-F7 or allele ZmCCT-OGD , and has an allelic The ear height of the gene ZmCCT-FO to be tested is lower than or the candidate is lower than the corn to be tested with the allele ZmCCT-F7 or the allele ZmCCT-OGD ; The nucleotide sequence of the ZmCCT allele ZmCCT-FO is sequence 14; The nucleotide sequence of the ZmCCT allele ZmCCT-OGD is sequence 2; The nucleotide sequence of the ZmCCT allele ZmCCT-F7 is sequence 1.

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