CN113087301B - Ectopic microorganism remediation method for river sewage - Google Patents

Abstract

The application belongs to the technical field of environmental microorganisms, and in particular relates to an ectopic microbial restoration method for river sewage; its technical points are as follows: S1. Take river sewage, test the ammonia nitrogen concentration C of the river sewage, and collect basic data; S2. Raoult bacteria for nitrification-aerobic denitrification are inoculated and expanded, and preliminarily enriched; S3, using the Raoul bacteria of efficient heterotrophic nitrification-aerobic denitrification in step S2 to perform preliminary treatment on the river sewage in step S1 , to obtain preliminarily treated river sewage; S4, filter the preliminarily treated river sewage obtained in step S3, and the filtrate is the repaired river sewage. The method uses parameters such as river sewage flow rate, river sewage ammonia nitrogen concentration and river sewage pH value to adjust the addition amount of ectopic microorganisms in the restoration of river sewage treatment, so that the standard quantification can be obtained, the precision of the input amount is improved, and excessive input is avoided. Causes insufficient aeration and affects the degradation rate.

Description

Ectopic microorganism remediation method for river sewage

Technical Field

The application belongs to the technical field of environmental microorganisms, and particularly relates to an ectopic microorganism remediation method for river sewage.

Background

At present, urban rivers in China are seriously polluted, and river sewage in most places is characterized by low organic matter concentration, relatively high nitrogen and phosphorus content and continuous change of water quality and water quantity along with seasons. In the prior art, although the traditional municipal sewage treatment process has higher treatment capacity on organic matters, the removal capacity on nitrogen and phosphorus is poorer. The microbial remediation technology is a new technology for remedying the water body pollution which is rapidly developed recently. The remediation technology mainly utilizes the absorption, transformation or degradation of the microorganisms to the pollutants in the water body, thereby achieving the purpose of slowing down or finally eliminating the pollution. The ectopic microorganism repair process treats the urban river sewage, converts organic nitrogen into ammonia nitrogen through aerobic autotrophic microorganisms, and finally releases the ammonia nitrogen to complete nitrogen circulation through the nitrification of nitrifying bacteria and the denitrification of denitrifying bacteria. The novel repairing process has low operation cost and small investment; the microorganism finally converts the pollutants into nitrogen, and the influence on the surrounding environment is small; the position of the processing object is not changed, and the operation management is simple and convenient; the treatment effect is good, and the pollutant removal efficiency is high. The heterotopic microorganism repairing technology has the defects that an electron acceptor is provided in an aeration mode, so that the operation cost is increased; when the amount of added nutrients is excessive, secondary pollution is caused. The heterotopic microorganism is very sensitive to temperature and ammonia nitrogen concentration, so the input amount of the heterotopic microorganism is a key factor for thoroughly treating the river sewage, and a standard method for judging the addition amount of the heterotopic microorganism in the river sewage treatment is lacked in the prior art.

In view of the defects of the prior art, the inventor develops a method for restoring ectopic microorganisms of river sewage by matching theoretical analysis and innovation based on years of abundant experience and professional knowledge of the materials and determining the input amount of the ectopic microorganisms in the river sewage treatment process under different environments according to the temperature, the pH value and the river sewage flow rate, so that the input amount of the ectopic microorganisms is standardized, and the phenomena of insufficient aeration by more throwing or low degradation rate by less throwing and the like are avoided.

Disclosure of Invention

The invention aims to provide a method for restoring ectopic microorganisms in river sewage, which can quantify the addition amount of the ectopic microorganisms in the river sewage restoration treatment through parameters such as river sewage flow rate, river sewage ammonia nitrogen concentration, river sewage pH value and the like, so that the standard quantification can be obtained, the precision of the input amount is improved, and the influence on the degradation rate due to insufficient aeration caused by excessive input is further avoided.

The technical purpose of the invention is realized by adopting the following technical scheme:

a method for restoring ectopic microorganisms of river sewage comprises the following operation steps:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage and collecting basic data;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, filtering the river sewage after the primary treatment obtained in the step S3, wherein the filtrate is the restored river sewage;

in which the step ofThe addition amount M of the Raoultella rufusi with efficient heterotrophic nitrification-aerobic denitrification in S3 is defined by the formula M ═ a × C²+ b × C + q, where a, b, q are regression parameters. According to the invention, the river sewage is treated by adopting Raoultella rapae of high-efficiency heterotrophic nitrification-aerobic denitrification, and the addition of microorganisms is determined according to the ammonia nitrogen concentration in the river sewage, so that the operation management is simple and convenient; the treatment effect is good, and the ammonia nitrogen content of the river sewage is effectively reduced.

Further, the basic data collection in step S1 includes collection of data of the pH S, the temperature T and the flow rate v of the river sewage. The microorganism adopted by the invention is very sensitive to the pH value and the temperature of the river sewage, and the slight change of the pH value and the temperature can have great influence on the degradation effect; and the flow rate of the river sewage can directly influence the input amount of microorganisms.

Further, a heterotrophic nitrifier culture medium is inoculated for primary screening, and then the bacterial strain with the heterotrophic nitrification-aerobic denitrification capability is screened out again by a bromothymol blue (BTB) plate screening method. And then the screened microorganisms are subjected to twice enrichment processes through a test tube and a conical flask respectively so as to improve the quantity and activity of the microorganisms.

Furthermore, the addition M of the Raoultella rapi for high-efficiency heterotrophic nitrification-aerobic denitrification is corrected and compensated by using an addition calculation model and a data compensation model. Since the pH value, the temperature and the flow rate of the river sewage affect the input amount of the microorganisms, the input amount of the microorganisms is corrected and compensated according to the pH value, the temperature and the flow rate of the river sewage in the treatment method provided by the invention.

Further, after the addition M of the Raoultella rapi for the high-efficiency heterotrophic nitrification-aerobic denitrification is corrected by using an addition calculation model, the addition of the Raoultella rapi for the high-efficiency heterotrophic nitrification-aerobic denitrification is M₁，M₁K · M; k is a total adjustment coefficient which is determined by the flow velocity v of the sewage in the river channel and the concentration C of ammonia nitrogen,

wherein b, m₁，m₂Is a regression parameter, the unit of the river sewage flow velocity v is m/s, and the unit of the ammonia nitrogen concentration C is mg/L. When the flow rate of the river sewage is high, more microorganisms need to be added, the flow rate is high, the aeration rate is low, the degradation result is not affected, more microorganisms can be added at the moment, and the degradation rate is improved.

Further, the addition amount of the Raoultella rapana of the high-efficiency heterotrophic nitrification-aerobic denitrification is M by utilizing a data compensation model₁After compensation, the addition amount of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M₂，M₂＝M₁+ N, N is a compensation value determined by the temperature of the river sewage, and N is-1.22T³+52.64T²-89T + 32; wherein T is the temperature of the river sewage and the unit is ℃. The nitrification-denitrification of the microorganisms is best at 25-30 ℃, and the decomposition rate is influenced when the temperature is lower or higher than the temperature, so that when the temperature is lower or higher than the temperature range, more microorganisms are added to ensure the decomposition rate.

Further, the pH value S of the river sewage is secondarily corrected for the amount of the laoultella effective heterotrophic nitrification-aerobic denitrification added in steps S3 and S5. When the pH value of the river sewage exceeds the optimal range of microorganisms, the decomposition rate of the river sewage is reduced, so that the sewage treatment rate is reduced, the sewage treatment efficiency is influenced, the input amount of the microorganisms is corrected through the change of the pH value, the sewage treatment rate can be ensured, and the influence on the turbidity of the river water is reduced.

Further, the addition amount of the high-efficiency heterotrophic nitrification-aerobic denitrification Raoultella subjected to secondary correction is M₃，M₃＝k·M₂K is a quadratic correction sum adjustment coefficient,

wherein v is the flow velocity of the river sewage, the unit is m/S, S is the pH value of the river sewage, and n is₁And n₂Are regression parameters.

Further, in step S4, a porous adsorbent is added and then filtered. The adsorbent is used for adsorbing residual microorganisms in the treated water and preventing the residual microorganisms from influencing the water quality of the river water.

Further, the porous adsorbent is porous ceramic or molecular sieve. By adopting porous ceramic or molecular sieve, on one hand, the residual microorganisms can be adsorbed, on the other hand, the water quality can be further purified, and the water can be recycled after being filtered.

In conclusion, the invention has the following beneficial effects:

according to the invention, the addition amount of the heterotopic microorganisms in the riverway sewage restoration treatment is determined by parameters such as the riverway sewage flow speed, the riverway sewage ammonia nitrogen concentration, the riverway sewage pH value and the like, so that the heterotopic microorganisms can be standardized, the precision of the input amount is improved, the riverway sewage treatment speed is improved, and a better treatment effect is obtained.

Detailed Description

To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the present invention provides an improved method for classifying soluble contaminant components based on the contaminant classification, and the specific implementation, characteristics and effects thereof are described in detail as follows.

Example 1: sewage treatment of bottom river in Wu-Cheng district of Changzhou city

The bottom river is located in a lake and ballast area of a Wuzhou city Wu-Ching district, and the surrounding residential areas are concentrated and accompanied by domestic sewage leakage and the condition that sewage interception is not in place, so that the water body environment is damaged, the black and odorous phenomenon is obvious, fishes die, the ammonia nitrogen in the water body is ultrahigh, and indexes such as oxidation-reduction potential, dissolved oxygen and the like exceed the water content standard of a river channel. The embodiment makes the following repairing method for the same:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage and collecting basic data;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, filtering the river sewage after the primary treatment obtained in the step S3, wherein the filtrate is the restored river sewage;

wherein the addition amount M of the Raoultella in step S3 is represented by the formula M ═ a × C²+ b × C + q, where a, b, q are regression parameters.

The detection data for treating the water sample by the method of the embodiment are as follows:

TABLE 1 Water sample data before and after treatment of the trench river

Example 2: double-channel river sewage treatment in Changzhou city

The double-ditch river is positioned on a new gate street in a clockwork area, the surrounding villages are gathered, the population is concentrated, and the domestic sewage and domestic garbage are poured into the river all the year round.

The embodiment makes the following repairing method for the same:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage, and acquiring the data of the pH value S, the temperature T and the river sewage flow velocity v of the river sewage;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, filtering the river sewage after the primary treatment obtained in the step S3, wherein the filtrate is the restored river sewage;

wherein the addition amount M of the Raoultella in step S3 is represented by the formula M ═ a × C²+ bXC + q, wherein a, b and q are regression parameters, and the addition M of the Raoultella for high-efficiency heterotrophic nitrification-aerobic denitrification is corrected by using an addition calculation model, and the corrected addition is M₁，M₁K is a sum adjustment coefficient;

namely, the addition amount of the Raoultella rapae of the efficient heterotrophic nitrification-aerobic denitrification in the step S3 is

Wherein b, m₁，m₂Is a regression parameter, the unit of the river sewage flow velocity v is m/s, and the unit of the ammonia nitrogen concentration C is mg/L.

The detection data for treating the water sample by the method of the embodiment are as follows:

TABLE 2 Water sample data before and after treatment of double rivers

	Oxidation reduction potential	Ammonia nitrogen/mg.L-1	Transparency	Dissolved oxygen/mg. L-1
					Before treatment	7	25	10.5	1.1
After treatment	68	3.5	58	8.5

Example 3: changzhou city Songjiang river sewage treatment

In a loose river, which is located in a new village in Zhouzhong villages, due to the fact that the rainfall amount is large in plum rain season, garbage wastewater in the garbage transfer station is discharged into a river channel, a large amount of fishes die, and strong pungent smell is emitted, and the following repairing method is implemented in the embodiment:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage, and acquiring the data of the pH value S, the temperature T and the river sewage flow velocity v of the river sewage;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, filtering the river sewage after the primary treatment obtained in the step S3, wherein the filtrate is the restored river sewage;

wherein the addition amount M of the Raoultella in step S3 is represented by the formula M ═ a × C²+ bXC + q, wherein a, b and q are regression parameters, and the addition M of the Raoultella for high-efficiency heterotrophic nitrification-aerobic denitrification is corrected by using an addition calculation model, and the corrected addition is M₁，M₁K is a sum adjustment coefficient;

and the addition of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M by utilizing a data compensation model₁After compensation, the addition amount of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M₂，M₂＝M₁+ N, N is a compensation value determined by the temperature of the river sewage, and N is-1.22T³+52.64T²-89T + 32; namely, the addition amount of the Raoultella rapae of the efficient heterotrophic nitrification-aerobic denitrification in the step S3 is

Wherein T is the temperature of the river sewage, and the unit is; b, m₁，m₂Is a regression parameter, the unit of the river sewage flow velocity v is m/s, the unit of the ammonia nitrogen concentration C is mg/L, n₁And n₂Are regression parameters.

The detection data for treating the water sample by the method of the embodiment are as follows:

TABLE 3 Water sample data before and after Songjiang river treatment

	Oxidation reduction potential	Ammonia nitrogen/mg.L-1	Transparency	Dissolved oxygen/mg. L-1
					Before treatment	-53	55.4	-1.9	0.6
After treatment	79	3.1	56	6.6

Example 4: changzhou Yanjia creek sewage treatment

The Yanjia creek is located in a lake pond Zhengming river industry centralized area, surrounding factory areas stand up, industrial wastewater steals into a river, and the method for repairing the Yanjia creek comprises the following steps:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage, and acquiring the data of the pH value S, the temperature T and the river sewage flow velocity v of the river sewage;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, filtering the river sewage after the primary treatment obtained in the step S3, wherein the filtrate is the restored river sewage;

wherein the addition amount M of the Raoultella in step S3 is represented by the formula M ═ a × C²+ bXC + q, wherein a, b and q are regression parameters, and the addition M of the Raoultella for high-efficiency heterotrophic nitrification-aerobic denitrification is corrected by using an addition calculation model, and the corrected addition is M₁，M₁K is a sum adjustment coefficient;

and the addition of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M by utilizing a data compensation model₁After compensation, the addition amount of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M₂，M₂＝M₁+ N, N is a compensation value determined by the temperature of the river sewage, and N is-1.22T³+52.64T²-89T + 32; simultaneously, the adding amount M of the efficient heterotrophic nitrification-aerobic denitrification Raoultella in the step S3 is determined according to the pH value S of the river sewage and the flow velocity v of the river sewage₂Performing secondary correction to obtain the addition M₃K is a quadratic correction sum adjustment coefficient,

namely, the addition amount of the Raoultella rapae of the efficient heterotrophic nitrification-aerobic denitrification in the step S3 is

Wherein v is the flow velocity of the river sewage, the unit is m/S, S is the pH value of the river sewage,

wherein T is the temperature of the river sewage, and the unit is; b, m₁，m₂Is a regression parameter, the unit of the ammonia nitrogen concentration C is mg/L, n₁And n₂Are regression parameters.

The detection data for treating the water sample by the method of the embodiment are as follows:

table 4 Water sample data before and after Yangjia creek treatment

Example 5: sewage treatment of Changzhou sightseeing park creek

The scenic spot garden creek is located in a district where the Wu-Chi enters and the town where the scenic spot garden is remotely observed, domestic sewage is discharged into the river, the life of residents in the district is seriously affected, and the embodiment makes the following repairing method for the scenic spot garden creek:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage, and acquiring the data of the pH value S, the temperature T and the river sewage flow velocity v of the river sewage;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, adding a porous ceramic adsorbent for adsorption after the primary treatment obtained in the step S3, and filtering the river sewage after adsorption to obtain filtrate, namely the restored river sewage;

wherein the addition amount M of the Raoultella in step S3 is represented by the formula M ═ a × C²+ bXC + q, wherein a, b and q are regression parameters, and the addition M of the Raoultella for high-efficiency heterotrophic nitrification-aerobic denitrification is corrected by using an addition calculation model, and the corrected addition is M₁，M₁K is a sum adjustment coefficient;

and the addition of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M by utilizing a data compensation model₁After compensation, the addition amount of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M₂，M₂＝M₁+ N, N is a compensation value determined by the temperature of the river sewage, and N is-1.22T³+52.64T²-89T + 32; simultaneously, the adding amount M of the efficient heterotrophic nitrification-aerobic denitrification Raoultella in the step S3 is determined according to the pH value S of the river sewage and the flow velocity v of the river sewage₂Performing secondary correction to obtain the addition M₃K is a quadratic correction sum adjustment coefficient,

namely, the addition amount of the Raoultella rapae of the efficient heterotrophic nitrification-aerobic denitrification in the step S3 is

Wherein v is the flow velocity of the river sewage, the unit is m/S, S is the pH value of the river sewage,

wherein T is the temperature of the river sewage, and the unit is;b，m₁，m₂is a regression parameter, the unit of the ammonia nitrogen concentration C is mg/L, n₁And n₂Are regression parameters.

The detection data for treating the water sample by the method of the embodiment are as follows:

TABLE 5 Water sample data before and after river and creek treatment in landscape garden

	Oxidation reduction potential	Ammonia nitrogen/mg.L-1	Transparency	Dissolved oxygen/mg. L-1
					Before treatment	6	12.6	14	0.7
After treatment	105	1.9	53	9.8

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (4)

1. The ectopic microorganism restoring method of the river sewage is characterized by comprising the following operation steps:

s1, taking river sewage, testing the ammonia nitrogen concentration C of the river sewage and collecting basic data;

s2, inoculating and expanding the efficient heterotrophic nitrification-aerobic denitrification Raoultella, and primarily enriching;

s3, carrying out primary treatment on the river sewage in the step S1 by adopting the Raoultella of the high-efficiency heterotrophic nitrification-aerobic denitrification in the step S2 to obtain primarily treated river sewage;

s4, filtering the river sewage after the primary treatment obtained in the step S3, wherein the filtrate is the restored river sewage;

wherein the addition amount M of the Raoultella in step S3 is represented by the formula M ═ a × C²+ bxC + q, wherein a, b, q are regression parameters; the basic data acquisition in the step S1 comprises the acquisition of data of the pH value S, the temperature T and the river sewage flow velocity v of the river sewage; correcting and compensating the addition M of the Raoultella rapae for the efficient heterotrophic nitrification-aerobic denitrification by using an addition calculation model and a data compensation model; after the addition M of the Raoultella for high-efficiency heterotrophic nitrification-aerobic denitrification is corrected by using an addition calculation model, the addition of the Raoultella for high-efficiency heterotrophic nitrification-aerobic denitrification is M₁，M₁K · M; k is a total adjustment coefficient which is determined by the flow velocity v of the sewage in the river channel and the concentration C of ammonia nitrogen,

wherein b, m₁，m₂Is a regression parameter, the unit of the river sewage flow velocity v is m/s, and the unit of the ammonia nitrogen concentration C is mg/L; the addition amount of the Raoultella rapae for the high-efficiency heterotrophic nitrification-aerobic denitrification by utilizing a data compensation model is M₁After compensation, the addition amount of the Raoultella rapae of the high-efficiency heterotrophic nitrification-aerobic denitrification is M₂，M₂＝M₁+ N, N is a compensation value determined by the temperature of the river sewage, and N is-1.22T³+52.64T²-89T + 32; wherein T is the temperature of the river sewage and the unit is ℃.

2. The method for remedying the ectopic microorganism in the river wastewater according to claim 1, wherein the addition amount M of the Raoultella eutropha nitrifying-aerobic denitrifying Raoultella in the step S3 is determined according to the pH value S of the river wastewater and the flow velocity v of the river wastewater₂Carrying out secondary correction; the addition amount of the high-efficiency heterotrophic nitrification-aerobic denitrification Raoultella subjected to secondary correction is M₃，M₃＝k·M₂K is a quadratic correction sum adjustment coefficient,

wherein v is the flow velocity of the river sewage, the unit is m/S, S is the pH value of the river sewage, and n is₁And n₂Are regression parameters.

3. The method for remediating ectopic microorganisms in river wastewater according to claim 1, wherein the step S4 comprises filtering the river wastewater after adding the porous adsorbent.

4. The ectopic microbial remediation method of river sewage according to claim 3, wherein the porous adsorbent is porous ceramic or molecular sieve.