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CN113082114A - Extraction and preparation process of medlar nutrient - Google Patents

Extraction and preparation process of medlar nutrient Download PDF

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CN113082114A
CN113082114A CN202110271692.3A CN202110271692A CN113082114A CN 113082114 A CN113082114 A CN 113082114A CN 202110271692 A CN202110271692 A CN 202110271692A CN 113082114 A CN113082114 A CN 113082114A
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宫晶晶
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Ningxia Qimeiren Biotechnology Co ltd
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    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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    • A61K2236/50—Methods involving additional extraction steps
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Abstract

The invention discloses an extraction and preparation process of medlar nutrient, which comprises the following steps: a: washing fructus Lycii with water, air drying, pulverizing with pulverizer, and sieving with 80 mesh sieve; b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, and stirring and mixing by using a stirrer to obtain an ethanol mixed solution; c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder; d: c, adding the solid powder in the step c into the composite liquid, carrying out ultrasonic extraction under the ultrasonic power of 150W, and then carrying out enzyme deactivation in a boiling water bath, centrifugation and a trifluoro trichloroethane method deproteinization method to obtain a medlar nutrient solution; e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract. According to the invention, the medlar powder is dissolved by 60% ethanol, and then the nutrients in the medlar are extracted by ultrasonic, so that the efficiency is high, the time is short, the working procedures are few, and the extraction cost of the medlar extract is greatly reduced.

Description

Extraction and preparation process of medlar nutrient
Technical Field
The invention belongs to the technical field of extraction of medlar nutrients, and particularly relates to an extraction and preparation process of medlar nutrients.
Background
The medlar is perennial shrub and grows on loess ditch sides and hillsides with deep soil layers, and has the effects of regulating immunity, reducing blood sugar, reducing blood fat, delaying senility, resisting fatigue and the like. The medlar seed oil is rich in linoleic acid, linolenic acid, oleic acid, vitamin E, carotene and other bioactive substances, and has the effects of reducing blood vessel cholesterol, preventing atherosclerosis, enhancing eyesight, preventing glaucoma, whitening, moistening, caring skin, reducing pigment and the like;
the existing extraction method of the medlar nutrient mainly adopts a medlar oil extraction method, a beta-carotene extraction method and a supercritical CO2 extraction separation method, the extraction time of the medlar nutrient is long, the working procedures are multiple, the efficiency is low, and the extraction cost of the medlar extract is higher.
Disclosure of Invention
The invention aims to solve the problems in the background art and provides an extraction and preparation process of medlar nutrient.
The technical scheme adopted by the invention is as follows:
an extraction preparation process of medlar nutrient comprises the following steps:
a: washing fructus Lycii with water, air drying, pulverizing with pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, and stirring and mixing by using a stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into the composite liquid, carrying out ultrasonic extraction under the ultrasonic power of 150W, and then carrying out enzyme deactivation in a boiling water bath, centrifugation and a trifluoro trichloroethane method deproteinization method to obtain a medlar nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
Preferably, the water washing in step a comprises the following steps:
adding water: mixing the medlar and the cleaning water according to the weight ratio of 1:30, and simultaneously stirring by using a stirrer for 2-3 min;
air drying: taking out the cleaned medlar, and quickly drying by using hot air;
and repeating the steps of adding water and air-drying, and ensuring that the medlar is cleaned for three times.
Preferably, the mass ratio of the medlar powder to the 60% ethanol in the step b is 1: 50-1: 30, the stirring time of the stirrer is 1h, and the temperature of the stirring liquid is controlled at 40-60 ℃.
Preferably, the compound liquid in the step c is prepared from lipase, protease, pectinase and cellulase in an installation mass ratio of 1:3:5:1, and the ultrasonic assistance time is 30-60 min.
Preferably, the time of the boiling water bath is 10min, the speed of centrifugation is 5000r/min, and the time of centrifugation is 10 min.
Preferably, the trifluoro trichloroethane deproteinization method comprises the following steps:
mixing: d, mixing the trifluorotrichloroethane method and the filtrate obtained in the step d in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
Preferably, the trifluorotrichloroethane process and step d are stirred for 30 min.
Preferably, both steps d and e are performed in a sterile environment.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. according to the invention, the medlar powder is dissolved by 60% ethanol, and then the nutrients in the medlar are extracted by ultrasonic, so that the efficiency is high, the time is short, the working procedures are few, and the extraction cost of the medlar extract is greatly reduced.
2. In the invention, the components of plant cell walls and cell membranes can be decomposed by lipase, protease, pectinase and cellulase in the complex liquid, thereby greatly improving the yield of the medlar extract.
3. In the invention, the dissolution of the medlar extract is promoted by an ultrasonic-enhanced mass transfer process, so that the extraction time of the medlar extract can be effectively shortened.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
10kg of dried medlar is equally divided into 10 parts and marked as No. 1-No. 10.
Example 1:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 40 ℃, and the stirring and mixing are carried out by using the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under the ultrasonic power of 150W, wherein the ultrasonic-assisted time is 30min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation, wherein the centrifugation speed is 5000r/min, the centrifugation time is 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 2:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 50 ℃, and the stirring and mixing are carried out by using the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under the ultrasonic power of 150W, wherein the ultrasonic-assisted time is 30min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation, wherein the centrifugation speed is 5000r/min, the centrifugation time is 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 3:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 60 ℃, and the stirring solution is stirred and mixed by the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under the ultrasonic power of 150W, wherein the ultrasonic-assisted time is 30min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation, wherein the centrifugation speed is 5000r/min, the centrifugation time is 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
Measuring the content of fructus Lycii polysaccharide in the obtained fructus Lycii nutrient extract, and recording
Example 4:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 40 ℃, and the stirring and mixing are carried out by using the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under 150W ultrasonic power for 45min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation at the speed of 5000r/min for 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 5:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 50 ℃, and the stirring and mixing are carried out by using the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under 150W ultrasonic power for 45min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation at the speed of 5000r/min for 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 6:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 60 ℃, and the stirring solution is stirred and mixed by the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under 150W ultrasonic power for 45min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation at the speed of 5000r/min for 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 7:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 40 ℃, and the stirring and mixing are carried out by using the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under the ultrasonic power of 150W, wherein the ultrasonic-assisted time is 60min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation, wherein the centrifugation speed is 5000r/min, the centrifugation time is 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 8:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 50 ℃, and the stirring and mixing are carried out by using the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under the ultrasonic power of 150W, wherein the ultrasonic-assisted time is 60min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation, wherein the centrifugation speed is 5000r/min, the centrifugation time is 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 9:
referring to fig. 1, a process for extracting and preparing medlar nutrient, taking No. 1 dry medlar, and installing the No. 1 dry medlar in the following steps:
a: washing fructus Lycii with water, repeating the washing with water and air drying for three times, washing with water, air drying, pulverizing with a pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, wherein the mass ratio of the Chinese wolfberry powder to the 60% ethanol is 1:40, the stirring time of a stirrer is 1h, the temperature of a stirring solution is controlled at 60 ℃, and the stirring solution is stirred and mixed by the stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into a composite liquid, wherein the composite liquid is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, carrying out ultrasonic extraction under the ultrasonic power of 150W, wherein the ultrasonic-assisted time is 60min, then carrying out enzyme deactivation in a boiling water bath for 10min, then carrying out centrifugation, wherein the centrifugation speed is 5000r/min, the centrifugation time is 10min, and finally carrying out deproteinization by using a trichlorotrifluoroethane method to obtain a Chinese wolfberry nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
The deproteinization method by the trichlorotrifluoroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
And measuring the content of the lycium barbarum polysaccharide in the prepared lycium barbarum nutrient extract, and recording.
Example 10:
the wolfberry fruit nutrient extract is prepared by using a wolfberry fruit oil and beta-carotene extraction method, and the content of wolfberry fruit polysaccharide is measured and recorded.
The wolfberry nutrient extract prepared by the above example and the content of wolfberry polysaccharide (wolfberry polysaccharide mass/dry wolfberry mass x 100%) measured are shown in the following table:
Figure BDA0002974649450000121
from the above examples, it can be seen that example 5 is the most preferred embodiment of the present invention, the temperature of the stirring liquid is controlled at 50 ℃, and the time of ultrasonic assistance is 45 min.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (8)

1. An extraction and preparation process of medlar nutrient is characterized by comprising the following steps: the method comprises the following steps:
a: washing fructus Lycii with water, air drying, pulverizing with pulverizer, and sieving with 80 mesh sieve;
b: b, adding the crushed Chinese wolfberry in the step a into 60% ethanol, and stirring and mixing by using a stirrer to obtain an ethanol mixed solution;
c: filtering the ethanol mixed solution in the step b, filtering, and then carrying out ethanol precipitation, dehydration and vacuum drying to obtain solid powder;
d: c, adding the solid powder in the step c into the composite liquid, carrying out ultrasonic extraction under the ultrasonic power of 150W, and then carrying out enzyme deactivation in a boiling water bath, centrifugation and a trifluoro trichloroethane method deproteinization method to obtain a medlar nutrient solution;
e: and d, concentrating the medlar nutrient solution in the step d, and drying in vacuum to obtain a medlar nutrient extract.
2. The extraction and preparation process of medlar nutrient as claimed in claim 1, wherein: the water washing in the step a comprises the following steps:
adding water: mixing the medlar and the cleaning water according to the weight ratio of 1:30, and simultaneously stirring by using a stirrer for 2-3 min;
air drying: taking out the cleaned medlar, and quickly drying by using hot air;
and repeating the steps of adding water and air-drying, and ensuring that the medlar is cleaned for three times.
3. The extraction and preparation process of medlar nutrient as claimed in claim 1, wherein: in the step b, the mass ratio of the medlar powder to the 60% ethanol is 1: 50-1: 30, the stirring time of the stirrer is 1h, and the temperature of the stirring liquid is controlled at 40-60 ℃.
4. The extraction and preparation process of medlar nutrient as claimed in claim 1, wherein: the compound liquid in the step c is composed of lipase, protease, pectinase and cellulase according to the installation mass ratio of 1:3:5:1, and the ultrasonic-assisted time is 30-60 min.
5. The extraction and preparation process of medlar nutrient as claimed in claim 1, wherein: the time of the boiling water bath is 10min, the centrifugation speed is 5000r/min, and the centrifugation time is 10 min.
6. The extraction and preparation process of medlar nutrient as claimed in claim 1, wherein: the method for deproteinizing by the trifluorotrichloroethane method comprises the following steps:
mixing: mixing the trifluorotrichloroethane method and the filtrate obtained in the step 4 in a volume ratio of 1:20, and fully mixing by using a stirrer;
centrifuging: and separating the mixed solution by using a centrifugal machine to obtain the medlar nutrient solution.
7. The extraction and preparation process of the medlar nutrient as claimed in claim 6, wherein the extraction and preparation process comprises the following steps: the trifluorotrichloroethane process and the stirring time of step d are 30 min.
8. The extraction and preparation process of medlar nutrient as claimed in claim 1, wherein: and d and e are carried out in a sterile environment.
CN202110271692.3A 2021-03-12 2021-03-12 Extraction and preparation process of medlar nutrient Pending CN113082114A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101041698A (en) * 2007-01-30 2007-09-26 李军 Preparation method of matrimony vine polysaccharide
CN101348529A (en) * 2008-09-08 2009-01-21 北京联合大学生物化学工程学院 A kind of extraction and separation method of Lycium barbarum polysaccharide
CN104311690A (en) * 2014-11-11 2015-01-28 济南凯因生物科技有限公司 Extraction method of lycium barbarum polysaccharides
CN108329399A (en) * 2018-05-15 2018-07-27 重庆市洲仨科技发展有限公司 A kind of extraction process of polysaccharides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101041698A (en) * 2007-01-30 2007-09-26 李军 Preparation method of matrimony vine polysaccharide
CN101348529A (en) * 2008-09-08 2009-01-21 北京联合大学生物化学工程学院 A kind of extraction and separation method of Lycium barbarum polysaccharide
CN104311690A (en) * 2014-11-11 2015-01-28 济南凯因生物科技有限公司 Extraction method of lycium barbarum polysaccharides
CN108329399A (en) * 2018-05-15 2018-07-27 重庆市洲仨科技发展有限公司 A kind of extraction process of polysaccharides

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Application publication date: 20210709