CN113049506A - Diagnosis system for quantitatively detecting fat content of excrement by using spectrophotometry - Google Patents
Diagnosis system for quantitatively detecting fat content of excrement by using spectrophotometry Download PDFInfo
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- CN113049506A CN113049506A CN202110153941.9A CN202110153941A CN113049506A CN 113049506 A CN113049506 A CN 113049506A CN 202110153941 A CN202110153941 A CN 202110153941A CN 113049506 A CN113049506 A CN 113049506A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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Abstract
The invention provides a diagnosis system for quantitatively detecting the fat content of excrement by utilizing a spectrophotometry, which comprises a rack, an objective table and a light splitting device, wherein the objective table is arranged on the rack, the light splitting device comprises a xenon lamp light source, a first lens group, a first diffraction grating, a second lens group, a sample cell, a second diffraction grating and a photomultiplier tube, the first lens group is arranged on the right side of the xenon lamp light source, the first diffraction grating is arranged on the right side of the first lens group, the sample cell is arranged below the xenon lamp light source, the second lens group is arranged between the sample cell and the first diffraction grating, the second diffraction grating is arranged on the right side of the sample cell, and the photomultiplier tube is arranged at the lower left corner of the second diffraction grating. The invention makes up the vacancy that the feces on the market cannot be quantitatively analyzed by an external analyzer, greatly reduces the workload of detection personnel and improves the accuracy of clinical analysis of the feces.
Description
Technical Field
The invention belongs to the technical field of spectral analysis, and particularly relates to a diagnostic system for quantitatively detecting the fat content of excrement by using a spectrophotometry method.
Background
Clinical in vitro diagnostic equipment has no quantitative detection method for the fat content of the fecal sample, the detection method for the fat content of the feces by medical personnel still stays on a microscopic visual inspection and chemical weighing method, the accuracy is low, the detection speed is slow, and the detection personnel need to contact the sample, so that the detection personnel can resist the method.
Therefore, a diagnostic system for quantitatively detecting the fat content of the excrement by using a spectrophotometry is needed, the analysis is performed by using the spectrophotometry, the vacancy that the excrement outside the bed analyzer cannot quantitatively analyze the fat of the excrement on the market is made up, the workload of detection personnel is greatly reduced, and the accuracy of clinical analysis of the excrement fat is improved.
Disclosure of Invention
In order to solve the technical problem, the invention provides a diagnosis system for quantitatively detecting the fat content of excrement by utilizing a spectrophotometry, which comprises a rack, an objective table and a light splitting device, wherein the objective table is arranged on the rack, the light splitting device comprises a xenon lamp light source, a first lens group, a first diffraction grating, a second lens group, a sample cell, a second diffraction grating and a photomultiplier tube, the first lens group is arranged on the right side of the xenon lamp light source, the first diffraction grating is arranged on the right side of the first lens group, the sample cell is arranged below the xenon lamp light source, the second lens group is arranged between the sample cell and the first diffraction grating, the second diffraction grating is arranged on the right side of the sample cell, and the photomultiplier tube is arranged at the lower left corner of the second diffraction grating.
Preferably, the xenon lamp light source, the first lens group and the first diffraction grating are arranged on the same horizontal straight line.
Preferably, the second lens group and the sample cell are both arranged at the lower left corner of the first diffraction grating.
Preferably, the first diffraction grating, the second lens group and the sample cell are arranged on the same inclined straight line.
Preferably, the sample cell and the diffraction grating are arranged on the same horizontal straight line.
Preferably, the first diffraction grating and the second diffraction grating are both concave diffraction gratings.
Preferably, the concave surface of the first diffraction grating faces the direction of the xenon lamp light source, and the concave surface of the second diffraction grating faces the direction of the sample cell.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, through arranging the rack, the objective table, the xenon lamp light source, the lens group I, the diffraction grating I, the lens group II, the sample cell, the diffraction grating II and the photomultiplier, the defect that feces on the market cannot be quantitatively analyzed by an external bed analyzer is overcome, the workload of detection personnel is greatly reduced, and the accuracy of clinical analysis of the feces fat is improved.
Drawings
Fig. 1 is a schematic structural view of the light-splitting apparatus of the present invention.
In the figure:
1-xenon lamp light source; 2-lens group one; 3-diffraction grating one; 4-lens group two; 5-a sample cell; 6-diffraction grating II; 7-photomultiplier tube.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
as shown in the attached figure 1, the diagnosis system for quantitatively detecting the fat content of excrement by utilizing a spectrophotometry method comprises a rack, an objective table and a light splitting device, wherein the objective table is arranged on the rack, the light splitting device comprises a xenon lamp light source 1, a lens group I2, a diffraction grating I3, a lens group II 4, a sample cell 5, a diffraction grating II 6 and a photomultiplier tube 7, the lens group I2 is arranged on the right side of the xenon lamp light source 1, the diffraction grating I3 is arranged on the right side of the lens group I2, the xenon lamp light source 1, the lens group I2 and the diffraction grating I3 are arranged on the same straight line of water, the sample cell 5 is arranged below the xenon lamp light source 1, the lens group II 4 is arranged between the sample cell 5 and the diffraction grating I3, the lens group II 4 and the sample cell 5 are both arranged at the lower left corner of the diffraction grating I3, and the diffraction grating I3, Two 4, the sample cell 5 of lens group set up on the straight line of same slope, 5 right sides of sample cell are provided with two 6 diffraction gratings, 5, two 6 settings of diffraction grating are on same horizontal straight line, the lower left corner of two 6 diffraction gratings is provided with photomultiplier 7.
Specifically, the first diffraction grating 3 and the second diffraction grating 6 are both concave diffraction gratings, the concave surface of the first diffraction grating 3 faces the direction of the xenon lamp light source 1, and the concave surface of the second diffraction grating 4 faces the direction of the sample cell 5.
Example 1:
because the transmittance of fat molecules is greatly different from other components in the excrement, the fat content in the excrement sample can be effectively measured by using a spectrophotometry method, firstly the excrement sample is extracted and weighed to obtain 0.05g-0.25g of excrement sample, the excrement sample is placed in a 25ml sample volumetric flask, 5ml of 2,2, 4-trimethylpentane (isooctane) solvent is firstly used for dissolving a test sample, then the test sample is diluted to the scale mark of the volumetric flask by the same solvent, then the test sample is led into a quartz colorimetric sample tube with the capacity of 3ml, a spectroscope is turned on, a light source of a xenon lamp light source 1 is emitted onto a diffraction grating I (3) through a lens group I (2), then diffraction is carried out through the diffraction grating I (3), the diffracted light source enters a sample cell 5 through a lens group II 4, the light source is emitted into a diffraction grating II 6 through the sample cell 5, and finally diffracted into a photomultiplier 7 through a diffraction grating II 6, the photomultiplier 7 transmits the electric signal to a computer for calculation, absorbance x can be obtained at the computer end, and the lambert-beer formula A = Kbc is shown, wherein A is absorbance, k is a molar absorption coefficient and is related to the property of an absorbing substance and the wavelength of incident light, b is the thickness of a solution through which light passes, c is the concentration of the absorbing substance, and the absorbance x is known to be positively related to the solubility of a sample diluent, so that a linear regression function is established for content prediction.
Then, performing a control group experiment to establish a linear regression equation, taking 100 parts of human body excrement samples, dividing the human body excrement samples into two parts with the same quantity, and measuring one part of the human body excrement samples by using the device to obtain absorbance x; the other part was tested by chemical weighing-Soxhlet extraction to determine the fat content y in g/g. Taking two experimental data as two parameters of the same set of data, processing the other 100 samples in the same way to finally obtain 100 sets of data, randomly using 75 sets of data to establish a linear regression equation, writing the linear regression equation into computer calculation software so that a computer can directly calculate a fat content result, predicting the fat content of the remaining 25 samples by using absorbance x, and if the prediction error is within 10%, the testing method is feasible.
Calculating the formula:
，
，
wherein x is the absorbance of the sample and y is the fat content.
The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention.
Claims (7)
1. A diagnostic system for quantitatively detecting the fat content of excrement by spectrophotometry is characterized in that, the diagnosis system comprises a frame, an object stage and light splitting equipment, wherein the object stage is arranged on the frame, the light splitting equipment comprises a xenon lamp light source (1), a first lens group (2), a first diffraction grating (3), a second lens group (4), a sample cell (5), a second diffraction grating (6) and a photomultiplier (7), the right side of the xenon lamp light source (1) is provided with a first lens group (2), the right side of the first lens group (2) is provided with a first diffraction grating (3), a sample cell (5) is arranged below the xenon lamp light source (1), a lens group II (4) is arranged between the sample cell (5) and the diffraction grating I (3), and a second diffraction grating (6) is arranged on the right side of the sample cell (5), and a photomultiplier (7) is arranged at the lower left corner of the second diffraction grating (6).
2. The diagnostic system for quantitatively determining the fat content of excrement by using a spectrophotometric method as claimed in claim 1, wherein the xenon lamp light source (1), the lens group one (2) and the diffraction grating one (3) are arranged on the same horizontal straight line.
3. The diagnostic system for quantitatively determining the fat content of excrement by utilizing the spectrophotometry as claimed in claim 1, wherein the second lens group (4) and the sample cell (5) are both arranged at the lower left corner of the first diffraction grating (3).
4. The diagnostic system for quantitatively determining the fat content of stool through a spectrophotometric method as set forth in claim 1, wherein the diffraction grating one (3), the lens group two (4) and the sample cell (5) are disposed on the same inclined straight line.
5. The diagnostic system for quantitatively determining the fat content of feces by means of spectrophotometry according to claim 1, wherein the sample cell (5) and the diffraction grating II (6) are disposed on the same horizontal straight line.
6. The diagnostic system for quantitatively determining the fat content of excrement by using the spectrophotometry as claimed in claim 1, wherein said diffraction gratings I (3) and II (6) are concave diffraction gratings.
7. The diagnostic system for quantitatively determining the fat content of feces by means of spectrophotometry according to claim 5, wherein the concave surface of the diffraction grating I (3) faces the direction of the xenon lamp light source (1), and the concave surface of the diffraction grating II (4) faces the direction of the sample cell (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110153941.9A CN113049506A (en) | 2021-02-04 | 2021-02-04 | Diagnosis system for quantitatively detecting fat content of excrement by using spectrophotometry |
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| CN202110153941.9A CN113049506A (en) | 2021-02-04 | 2021-02-04 | Diagnosis system for quantitatively detecting fat content of excrement by using spectrophotometry |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101308092A (en) * | 2007-05-16 | 2008-11-19 | 株式会社岛津制作所 | Fluorescence spectrophotometer |
| WO2013046418A1 (en) * | 2011-09-30 | 2013-04-04 | 株式会社 島津製作所 | Spectrophotofluorometer |
| US20150138546A1 (en) * | 2013-11-19 | 2015-05-21 | Shimadzu Corporation | Spectrophotometer including photodiode array |
| CN106338341A (en) * | 2015-07-09 | 2017-01-18 | 株式会社岛津制作所 | Spectroscopic detector |
| CN108732146A (en) * | 2017-04-19 | 2018-11-02 | 株式会社岛津制作所 | Quantum yield computational methods, sepectrophotofluorometer and storage medium |
| CN110824080A (en) * | 2018-08-10 | 2020-02-21 | 株式会社岛津制作所 | Spectroscopic detector |
-
2021
- 2021-02-04 CN CN202110153941.9A patent/CN113049506A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101308092A (en) * | 2007-05-16 | 2008-11-19 | 株式会社岛津制作所 | Fluorescence spectrophotometer |
| WO2013046418A1 (en) * | 2011-09-30 | 2013-04-04 | 株式会社 島津製作所 | Spectrophotofluorometer |
| US20150138546A1 (en) * | 2013-11-19 | 2015-05-21 | Shimadzu Corporation | Spectrophotometer including photodiode array |
| CN106338341A (en) * | 2015-07-09 | 2017-01-18 | 株式会社岛津制作所 | Spectroscopic detector |
| CN108732146A (en) * | 2017-04-19 | 2018-11-02 | 株式会社岛津制作所 | Quantum yield computational methods, sepectrophotofluorometer and storage medium |
| CN110824080A (en) * | 2018-08-10 | 2020-02-21 | 株式会社岛津制作所 | Spectroscopic detector |
Non-Patent Citations (2)
| Title |
|---|
| 刘永乐等: "用分光光度法测定发酵液中液体脂肪的含量", 《食品工业科技》 * |
| 陈邵苹等: "紫外分光光度法测定鱼粉中的脂肪", 《福建分析测试》 * |
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