CN112986232A - Methods and reagents for assessing gestational diabetes - Google Patents
Methods and reagents for assessing gestational diabetes Download PDFInfo
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- CN112986232A CN112986232A CN202110360021.4A CN202110360021A CN112986232A CN 112986232 A CN112986232 A CN 112986232A CN 202110360021 A CN202110360021 A CN 202110360021A CN 112986232 A CN112986232 A CN 112986232A
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 90
- 208000004104 gestational diabetes Diseases 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000001514 detection method Methods 0.000 claims abstract description 36
- 210000001124 body fluid Anatomy 0.000 claims abstract description 34
- 239000010839 body fluid Substances 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000002347 injection Methods 0.000 claims abstract description 26
- 239000007924 injection Substances 0.000 claims abstract description 26
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims abstract description 9
- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 9
- 239000004571 lime Substances 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
- 239000008103 glucose Substances 0.000 claims description 19
- 239000008213 purified water Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 4
- 210000002700 urine Anatomy 0.000 claims description 4
- 239000011449 brick Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims 6
- 230000001681 protective effect Effects 0.000 claims 2
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 14
- 238000011156 evaluation Methods 0.000 abstract description 4
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 210000000941 bile Anatomy 0.000 abstract 2
- 230000035935 pregnancy Effects 0.000 description 11
- 210000000013 bile duct Anatomy 0.000 description 5
- 208000002705 Glucose Intolerance Diseases 0.000 description 3
- 201000009104 prediabetes syndrome Diseases 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 201000001421 hyperglycemia Diseases 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 206010018429 Glucose tolerance impaired Diseases 0.000 description 1
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 1
- 206010056997 Impaired fasting glucose Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
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Abstract
本发明提供用于评估妊娠糖尿病的方法和试剂，涉及妊娠糖尿病评估检测技术领域。该用于评估妊娠糖尿病的方法和试剂，包括试剂管管体，所述试剂管管体的顶部旋合有密封螺帽，所述试剂管管体内壁的顶部固定连接有连接部，所述连接部底部的中心固定连接有内胆管体；所述试剂管管体的中心设置有石灰粉，所述内胆管体的中心设置有检测试剂；所述连接部顶部的中心开设有与内胆管体相对应的体液注射孔，所述连接部顶部的一侧开设有与试剂管管体相对应的水体注射孔。通过设计简单的检测试剂和快速检测方法，可以在妊娠期女性不确定自身是否患有糖尿病时，快速进行初步的诊断和评估，并根据检测结果判断是否有必要进行进一步检查，值得大力推广。
The present invention provides methods and reagents for assessing gestational diabetes, and relates to the technical field of assessment and detection of gestational diabetes. The method and reagent for evaluating gestational diabetes include a reagent tube body, the top of the reagent tube body is screwed with a sealing screw cap, and the top of the inner wall of the reagent tube is fixedly connected with a connecting portion, and the connection is The center of the bottom of the part is fixedly connected with an inner bile tube body; the center of the reagent tube body is provided with lime powder, and the center of the inner bile tube body is provided with a detection reagent; For the corresponding body fluid injection holes, a water injection hole corresponding to the body of the reagent tube is opened on one side of the top of the connecting portion. By designing simple detection reagents and rapid detection methods, when pregnant women are not sure whether they have diabetes, they can quickly carry out preliminary diagnosis and evaluation, and determine whether further examination is necessary based on the test results, which is worthy of vigorous promotion.
Description
Technical Field
The invention relates to the technical field of gestational diabetes evaluation and detection, in particular to a method and a reagent for evaluating gestational diabetes.
Background
Gestational diabetes mellitus includes gestational diabetes (i.e., diabetes mellitus with pregnancy), and gestational diabetes; gestational diabetes is hyperglycemia of varying degrees due to varying degrees of impaired glucose tolerance and diabetes that is found or developed during pregnancy. By definition, this group of diabetes includes both pre-pregnancy, i.e. existing but not diagnosed during pregnancy, and the development of hyperglycemia with pregnancy, and it includes both diabetes, as well as impaired glucose tolerance and impaired fasting glucose; some patients have been diagnosed with diabetes or impaired glucose tolerance prior to pregnancy, and continue to present or progress after pregnancy. In order to conveniently research the relationship between pregnancy and diabetes and improve the clinical diagnosis and prevention level, the gestational diabetes refers to the general term of abnormal glucose tolerance, abnormal fasting blood glucose and diabetes found or suffered during pregnancy, the poor control of the gestational diabetes can cause serious maternal and fetal recent and distant complications and complications, and the current research shows that age, obesity, race, poor fertility history and diabetes family history are main factors influencing the gestational diabetes.
Gestational diabetes is a special type of diabetes suffered by women during pregnancy, and the onset of diabetes is in pregnancy, so extra care and attention are needed for detecting the symptoms of the pregnant women, the most common detection modes of gestational diabetes at present are blood sugar determination, urine examination and determination, glycosylated hemoglobin determination and the like, but the existing detection reagent and detection method are complex, are inconvenient for quick detection and self-detection, can only be detected and judged by professional medical personnel, and cannot perform preliminary diagnosis and evaluation when the pregnant women are uncertain whether to suffer from the diabetes, so a new method and reagent for evaluating the gestational diabetes are developed.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method and a reagent for evaluating gestational diabetes, and solves the problems that the existing detection reagent and detection method are complex, are inconvenient for quick detection and self-detection, and can only be detected and judged by professional medical care personnel.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the reagent for evaluating the gestational diabetes comprises a reagent tube body, wherein a sealing nut is screwed on the top of the reagent tube body, the top of the inner wall of the reagent tube body is fixedly connected with a connecting part, and the center of the bottom of the connecting part is fixedly connected with an inner container tube body;
lime powder is arranged in the center of the reagent tube body, and a detection reagent is arranged in the center of the inner container tube body;
a body fluid injection hole corresponding to the inner container tube body is formed in the center of the top of the connecting part, a water body injection hole corresponding to the reagent tube body is formed in one side of the top of the connecting part, and a first sealing plug and a second sealing plug are respectively mounted at the tops of the body fluid injection hole and the water body injection hole;
the outer wall of the reagent tube body is sleeved with a tube body protecting sleeve, and a transparent observation window is arranged at the front end of the outer wall of the tube body protecting sleeve.
Preferably, the reagent tube body, the connecting part and the inner container tube body are all made of explosion-proof glass materials.
Preferably, the reagent tube body, the connecting part and the inner container tube body are of an integrated structure.
Preferably, the first sealing plug and the second sealing plug are both made of rubber.
Preferably, the tube protecting sleeve and the transparent observation window are both made of high-temperature-resistant rubber materials.
The use method of the reagent for evaluating the gestational diabetes comprises the following specific steps:
s1, when the reagent is used, firstly, a person to be tested drinks a cup of glucose solution with higher sugar content, and after the glucose solution is drunk for a period of time, a plastic container is used for connecting a cup of body fluid of the person to be tested for later use;
s2, opening a sealing nut of the reagent, sucking a tube of body fluid by using an injection tube, and injecting the body fluid into the inner container tube body through a first sealing plug and a body fluid injection hole;
s3, taking a new injector, sucking a tube of purified water, and injecting the sucked purified water into the tube body of the reagent tube through a second sealing plug and a water body injection hole;
s4, after the purified water is contacted with the trace lime powder in the reagent tube body, the purified water can quickly heat, so that the inner container tube body can be quickly heated;
s5, when the glucose content in the body fluid of the person to be detected is higher, the detection reagent in the inner bile duct gradually turns brick red after being heated, and a certain precipitate is generated; on the contrary, when the glucose content in the body fluid of the person to be detected is lower or is at a normal level, the detection reagent in the inner bile duct does not change color or changes color slightly after being heated.
Preferably, the preparation ratio of glucose to water in the glucose solution in S1 is 1 to 4.
Preferably, the body fluid of the person to be tested in S1 is urine.
Preferably, the body fluid in S2 is then gently shaken for 10-20 seconds after being injected into the inner bile duct body.
(III) advantageous effects
The present invention provides methods and reagents for assessing gestational diabetes. The method has the following beneficial effects:
1. the method and the reagent for evaluating the gestational diabetes mellitus can preliminarily evaluate whether the pregnant woman suffers from the disease or not according to the change condition of the pregnant woman body fluid after detection by designing the simple detection reagent, the whole detection process does not need to be operated by professional personnel, and the operation process is simple, convenient and fast.
2. The method and the reagent for evaluating the gestational diabetes can quickly perform preliminary diagnosis and evaluation when the pregnant women are uncertain whether the pregnant women suffer from the diabetes or not by designing a simple detection reagent and a quick detection method, and judge whether further examination is necessary or not according to a detection result, so that the integral working efficiency is greatly improved, and the method and the reagent are worthy of great popularization.
Drawings
FIG. 1 is a front view structural diagram of a reagent vessel for evaluating gestational diabetes according to the present invention;
FIG. 2 is a sectional view of a reagent vessel for evaluating gestational diabetes mellitus according to the present invention.
Wherein, 1, a reagent tube body; 2. a sealing nut; 3. a connecting portion; 4. a liner tube body; 5. lime powder; 6. a detection reagent; 7. a body fluid injection port; 8. a water body injection hole; 9. a first sealing plug; 10. a second sealing plug; 11. a pipe body protecting jacket; 12. a transparent viewing window.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
as shown in fig. 1-2, the reagent for evaluating gestational diabetes according to the embodiments of the present invention includes a reagent tube body 1, a sealing nut 2 is screwed on the top of the reagent tube body 1, a connecting part 3 is fixedly connected to the top of the inner wall of the reagent tube body 1, and a liner tube body 4 is fixedly connected to the center of the bottom of the connecting part 3;
the center of the reagent tube body 1 is provided with lime powder 5, and a trace amount of lime powder 5 can quickly generate heat after meeting water so as to be used for quickly heating the detection reagent 6 in the inner container tube body 4, so that the change condition of the detection reagent 6 can be conveniently observed, the center of the inner container tube body 4 is provided with the detection reagent 6, and the detection reagent 6 can be composed of a Peltier reagent, but not only the Peltier reagent, but also the detection reagent 6 can be any reagent for quickly detecting diabetes related;
a body fluid injection hole 7 corresponding to the inner container tube body 4 is formed in the center of the top of the connecting part 3, a water body injection hole 8 corresponding to the reagent tube body 1 is formed in one side of the top of the connecting part 3, and a first sealing plug 9 and a second sealing plug 10 are respectively mounted at the tops of the body fluid injection hole 7 and the water body injection hole 8;
the outer wall of reagent pipe body 1 has cup jointed body lag 11, and the front end of body lag 11 outer wall is provided with transparent observation window 12.
The first sealing plug 9 and the second sealing plug 10 are both made of rubber, and the first sealing plug 9 and the second sealing plug 10 made of rubber not only can play a role in sealing, but also are convenient for the insertion of an injection tube.
The use method of the reagent for evaluating the gestational diabetes comprises the following specific steps:
s1, when the reagent is used, firstly, a person to be tested drinks a cup of glucose solution with higher sugar content, and after the glucose solution is drunk for a period of time, a plastic container is used for connecting a cup of body fluid of the person to be tested for later use;
s2, opening a sealing nut 2 of the reagent, sucking a tube of body fluid by using an injection tube, and injecting the body fluid into the liner tube body 4 through a first sealing plug 9 and a body fluid injection hole 7;
s3, taking a new injector, sucking a tube of purified water, and injecting the sucked purified water into the reagent tube body 1 through the second sealing plug 10 and the water body injection hole 8;
s4, after the purified water is contacted with the trace lime powder 5 in the reagent tube body 1, the purified water can quickly heat, so that the inner container tube body 4 can be quickly heated;
s5, when the glucose content in the body fluid of the person to be detected is higher, the detection reagent 6 in the liner tube body 4 gradually turns brick red after being heated, and a certain precipitate is generated; on the contrary, when the glucose content in the body fluid of the person to be detected is lower or is at a normal level, the detection reagent 6 in the liner tube body 4 does not change color or changes color slightly after being heated.
The preparation ratio of glucose to water in the glucose solution in S1 is 1: 4.
The body fluid of the person to be tested in S1 is specifically urine.
The body fluid in the S2 is slightly shaken for 10-20 seconds in the subsequent process of being injected into the inner bile duct body 4, and the detection reagent 6 in the inner bile duct body 4 can be quickly fused with the body fluid by slight shaking for 10-20 seconds, so that the detection quality is ensured.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. Reagent for assessing gestational diabetes, comprising a reagent tube body (1), characterized in that: a sealing nut (2) is screwed on the top of the reagent tube body (1), a connecting part (3) is fixedly connected to the top of the inner wall of the reagent tube body (1), and an inner container tube body (4) is fixedly connected to the center of the bottom of the connecting part (3);
lime powder (5) is arranged in the center of the reagent tube body (1), and a detection reagent (6) is arranged in the center of the inner container tube body (4);
a body fluid injection hole (7) corresponding to the inner container tube body (4) is formed in the center of the top of the connecting part (3), a water body injection hole (8) corresponding to the reagent tube body (1) is formed in one side of the top of the connecting part (3), and a first sealing plug (9) and a second sealing plug (10) are respectively installed at the tops of the body fluid injection hole (7) and the water body injection hole (8); the outer wall of the reagent tube body (1) is sleeved with a tube body protective sleeve (11), and a transparent observation window (12) is arranged at the front end of the outer wall of the tube body protective sleeve (11).
2. The agent for evaluating gestational diabetes according to claim 1, wherein: the reagent tube body (1), the connecting part (3) and the inner container body (4) are all made of explosion-proof glass materials.
3. The agent for evaluating gestational diabetes according to claim 1, wherein: the reagent tube body (1), the connecting part (3) and the inner container tube body (4) are of an integrated structure.
4. The agent for evaluating gestational diabetes according to claim 1, wherein: the first sealing plug (9) and the second sealing plug (10) are both made of rubber materials.
5. The agent for evaluating gestational diabetes according to claim 1, wherein: the tube protecting sleeve (11) and the transparent observation window (12) are both made of high-temperature-resistant rubber materials.
6. The use of an agent for evaluating gestational diabetes according to any one of claims 1 to 5, wherein: the method comprises the following specific steps:
s1, when the reagent is used, firstly, a person to be tested drinks a cup of glucose solution with higher sugar content, and after the glucose solution is drunk for a period of time, a plastic container is used for connecting a cup of body fluid of the person to be tested for later use;
s2, opening a sealing nut (2) of the reagent, sucking a tube of body fluid by using an injection tube, and injecting the body fluid into the inner container tube body (4) through a first sealing plug (9) and a body fluid injection hole (7);
s3, taking a new injector, sucking a tube of purified water, and injecting the sucked purified water into the reagent tube body (1) through a second sealing plug (10) and a water body injection hole (8);
s4, after the purified water is contacted with the trace lime powder (5) in the reagent tube body (1), the purified water can quickly heat, so that the inner container tube body (4) can be quickly heated;
s5, when the glucose content in the body fluid of the person to be detected is higher, the detection reagent (6) in the liner tube body (4) gradually turns brick red after being heated, and a certain precipitate is generated; on the contrary, when the glucose content in the body fluid of the person to be detected is lower or is at a normal level, the detection reagent (6) in the liner tube body (4) can not change color or change color slightly after being heated.
7. The use method of the reagent for evaluating gestational diabetes according to claim 6, wherein: the preparation ratio of the glucose to the water in the glucose solution in the S1 is 1 to 4.
8. The use of the agent for evaluating gestational diabetes according to claim 6, wherein: the body fluid of the person to be tested in the S1 is urine.
9. The use method of the reagent for evaluating gestational diabetes according to claim 6, wherein: the body fluid in the S2 is slightly shaken for 10 to 20 seconds after being injected into the liner tube body (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110360021.4A CN112986232A (en) | 2021-04-02 | 2021-04-02 | Methods and reagents for assessing gestational diabetes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110360021.4A CN112986232A (en) | 2021-04-02 | 2021-04-02 | Methods and reagents for assessing gestational diabetes |
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| CN112986232A true CN112986232A (en) | 2021-06-18 |
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| CN202110360021.4A Pending CN112986232A (en) | 2021-04-02 | 2021-04-02 | Methods and reagents for assessing gestational diabetes |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2054905U (en) * | 1989-10-12 | 1990-03-21 | 中国人民解放军第三十五医院 | Urine self-detecting case |
| CN1448716A (en) * | 2002-03-28 | 2003-10-15 | 富士胶片株式会社 | Humoral testing unit |
| CN205426793U (en) * | 2015-12-16 | 2016-08-03 | 无锡亿仁肿瘤医院有限公司 | Novel index detection kit for diabetes mellitus |
| CN207591899U (en) * | 2017-12-06 | 2018-07-10 | 郑晓东 | A kind of endocrine submitting test tube |
| CN211412107U (en) * | 2019-08-01 | 2020-09-04 | 黄淮学院 | Auxiliary device for tumor drug synthesis test |
-
2021
- 2021-04-02 CN CN202110360021.4A patent/CN112986232A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2054905U (en) * | 1989-10-12 | 1990-03-21 | 中国人民解放军第三十五医院 | Urine self-detecting case |
| CN1448716A (en) * | 2002-03-28 | 2003-10-15 | 富士胶片株式会社 | Humoral testing unit |
| CN205426793U (en) * | 2015-12-16 | 2016-08-03 | 无锡亿仁肿瘤医院有限公司 | Novel index detection kit for diabetes mellitus |
| CN207591899U (en) * | 2017-12-06 | 2018-07-10 | 郑晓东 | A kind of endocrine submitting test tube |
| CN211412107U (en) * | 2019-08-01 | 2020-09-04 | 黄淮学院 | Auxiliary device for tumor drug synthesis test |
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