Disclosure of Invention
The invention relates to an automatic coating machine, which comprises a flat plate loading frame (figure 2), a coating frame loading frame (figure 3), a host machine shell (reference numeral 31), a coating frame (figure 5), a flat plate conveying system (figure 4), a coating frame conveying system, a control system and the like. The device has the characteristics of simple and quick operation, simple structure and low cost.
The invention designs a plate loading frame (figure 2) which consists of a plate loading cabin (reference numeral 11) and a chassis (reference numeral 14), and can conveniently place a plate with an inner package and a culture medium added in the plate loading cabin (reference numeral 11). Inverting the culture medium plate completely packaged, cutting open the bottom seal of the plate inner packaging bag, inserting the plate loading cabin into the packaging bag, inverting the loading frame, pushing the loading frame into a host machine plate inlet (reference numeral 5), fixing the plate loading frame at a constant position through a chassis (reference numeral 14), and enabling the plate to freely slide up and down in the loading cabin (reference numeral 11). The plate loading frame has the following characteristics: the diameter of the inner diameter or the inscribed circle of the fixing rod (12) of the flat loading cabin (11) is more than 0.2mm larger than the outer diameter of the upper shell of the flat used, and the diameter of the outer diameter or the inscribed circle of the fixing rod (12) of the lower end of the flat loading cabin (11) or the inscribed circle of the fixing rod (12) is more than 0.2mm smaller than the diameter of the flat inlet (5) of the host (31); the lower end of the plate loading frame is provided with a chassis (reference numeral 14), the chassis (reference numeral 14) is annular, the outer diameter is larger than the outer diameter of a plate inlet (reference numeral 5) of a host (reference numeral 31), and the inner diameter is not smaller than the inner diameter of a plate loading cabin or the diameter of an inscribed circle of a fixing rod (reference numeral 12).
The invention relates to a coating rack loading rack and a coating rack loading groove device, wherein the coating rack loading rack has similar functions with a flat plate loading rack and comprises a coating rack loading cabin (reference numeral 15) and a chassis (reference numeral 18). Coating frame loading groove device, the design is on host computer top right side, loading groove both sides (reference numeral 13) inboard distance is 82mm, be greater than the coating frame total length, the height design is 12mm, it is equivalent with the width of coating frame locating arm, design two bosss (reference numeral 16) in loading groove both sides, make the coating frame of packing accurately confirm to be located the loading groove, can make coating arm and loading groove bottom form certain clearance simultaneously, cut coating frame wrapping bag bottom and seal, insert the wrapping bag to the coating frame loading frame, make dead lever (reference numeral 17) follow the clearance passes, make the coating frame fix in the coating frame loading frame, can freely slide from top to bottom, but can not overturn or around or move, make the coating frame wrapping bag cover outside the coating frame loading frame simultaneously, play sealed pollution prevention's effect. Then the loading frame loaded with the coating frame is placed at the inlet position of the coating frame of the host machine and fixed.
The invention designs a flat plate conveying system which comprises a flat plate conveying cabin (reference numeral 19), a flat plate fixing disc (reference numeral 21), a bracket (reference numeral 25) and an electric push rod.
The flat plate conveying cabin (reference numeral 19) comprises a cabin body and a cabin cover (reference numeral 23), the cabin body plays a role of transporting flat plates left and right, meanwhile, a supporting platform is provided for transporting the flat plates up and down, and the cabin cover (reference numeral 23) plays a role of fixing a flat plate (reference numeral 21) for conveying the flat plates up and down. The flat plate conveying cabin body and the movable cabin cover (reference numeral 23) are characterized in that the cabin body is provided with a groove or a convex edge (reference numeral 20) which is matched with the convex edge or the groove (reference numeral 24) on the cabin cover (reference numeral 23), and the cabin cover (reference numeral 23) can slide in the cabin body under the driving of a motor and a screw rod. In order to achieve the functions of claim 4 and claim 10, the invention provides a flat plate conveying cabin cover (reference numeral 23), wherein structures such as a flat plate fixing disc driving motor fixing groove (reference numeral 32), a flat plate support driving motor fixing groove (reference numeral 34), a flat plate support fixing groove (reference numeral 33), a wire hole (reference numeral 35) and the like are designed for wire transmission.
The flat fixed disc (reference numeral 21) is connected with a motor output shaft fixed on the hatch (reference numeral 23) and can rotate under the drive of the motor to finish coating. The flat plate fixing frame is characterized in that the inner diameter of the upper opening of the flat plate fixing disc (reference numeral 21) is larger than the outer diameter of the lower flat plate shell, so that the lower flat plate shell can be fixed on the flat plate fixing disc (reference numeral 21), the lower surface of the flat plate fixing disc (reference numeral 21) is connected with a motor output shaft and can drive the flat plate fixing disc (reference numeral 21) and a flat plate to rotate together, and holes (reference numeral 22) are formed in the lower surface of the flat plate fixing frame.
And a bracket (reference numeral 25) is further designed in the plate conveying bin, and when the plate is coated, the bracket (reference numeral 25) can move downwards to support the cabin cover (reference numeral 23) so that the plate contacts the coating rack. When the flat plate is taken out, the bracket (reference numeral 25) can also move upwards to penetrate through the hole (reference numeral 22) below the flat plate fixed disc (reference numeral 21), and after the lower shell and the upper shell of the flat plate are supported to be closed, the flat plate is taken out, so that the contact between the flat plate and the outside is reduced, and the pollution is reduced. The support (reference numeral 25) has the following features: the bracket (reference numeral 25) is connected with a motor, the bracket (reference numeral 25) can be lifted, the bracket (reference numeral 25) is lifted, the flat plate can be supported through the hole (reference numeral 22) on the flat plate fixing disc (reference numeral 21), and when the bracket (reference numeral 25) is lowered, the hatch cover (reference numeral 23) of claim 4 can be supported to drive the flat plate fixing frame and the flat plate to ascend.
The invention designs a coating frame which can uniformly coat a sample on a flat plate and has the following characteristics that the coating frame consists of three parts, namely a sample adding port (reference numeral 7), a positioning arm (reference numeral 27) and a coating arm (reference numeral 28); the positioning arm (reference numeral 27) is of a T-shaped structure and is positioned at the upper end of the sample adding port (reference numeral 7); with this structure, the coating rack is suspended on the conveyor belt, and the conveyance of the coating rack is realized. The upper end of the coating arm (reference numeral 28) is provided with a groove (reference numeral 29) which is communicated with the sample adding port (reference numeral 26) of the coating frame, the volume of the groove (reference numeral 29) is more than 1ml, the coating surface at the lowest part of the coating arm (reference numeral 28) is in a circular arc shape, the bottom of the groove (reference numeral 29) is provided with a slit (reference numeral 30), the intersection line of the slit (reference numeral 30) and the bottom of the coating arm (reference numeral 28) can be controlled by controlling the height of the intersection line and the thickness of the coating layer, a sample flows into the groove (reference numeral 29) of the coating arm (reference numeral 28) through the sample adding port (reference numeral 26) of the coating frame and is uniformly coated on a flat plate through the slit (reference numeral 30), and the thickness of the coating layer can be controlled by controlling the height difference of the.
The invention designs a coating rack conveying system which comprises two conveying belts, wherein a coating arm (reference numeral 28) can pass through a space between the two conveying belts, and a coating rack is hung on the conveying belts through a positioning arm (reference numeral 27) and conveyed to a coating position to stop. After coating is completed, the conveyor belt continues to run to transport the coating rack out of the facility. Then, a new coating frame is replaced to repeat the actions, so that the mutual cross contamination is avoided. The conveyor system has the feature that the conveyor belt pitch is greater than the width of the coating arm (reference numeral 28) and less than the width of the positioning arm (reference numeral 27). The conveyor belt is designed to be wider at the inlet of the coating stand (reference numeral 6) than at the outlet of the coating stand (reference numeral 9), gradually tightening up the coating stand. The coating rack can move along the direction of the conveyor belt, can also move up and down in the gap of the conveyor belt, but can not move along other directions. Therefore, the coating frame can avoid scratching the flat plate due to the uneven flat plate in the coating process. Meanwhile, in order to ensure that the coating rack is not blocked when falling into the coating rack conveying system and the coating rack reaches the coating position, the coating rack is tightly fixed through the conveying system and cannot be conveyed out of the equipment at the outlet position due to the blocking of the garbage bag.
The invention also designs a coating rack locking device which is positioned below the inlet (reference number 6) of the coating rack and above the conveyor belt, when the locking device is opened, the lowermost coating rack enters the coating rack conveying system, and the last coating rack can be locked to avoid mutual influence.
The invention also designs a set of control system which controls the operation of each part through circuit design and internal programs. After a user respectively loads a flat plate and a coating rack (generally, 10 bags are arranged in the flat plate loading rack and the coating rack loading rack) into the corresponding flat plate loading rack and the coating rack loading rack, the flat plate loading rack and the coating rack are placed into a flat plate inlet (reference numeral 5) and a coating rack inlet (reference numeral 6) corresponding to a host (reference numeral 31), the user presses a start-stop (reference numeral 2) button, the equipment starts to operate, the sample is added after the user hears a prompt tone, the user adds a sample, presses a completion key (reference numeral 4), the equipment finishes coating, the corresponding coating rack and the flat plate are conveyed out of the equipment, then a new set is conveyed, the user hears the prompt tone to add the sample again, presses a completion key (reference numeral 4), the user presses the start-stop key (reference numeral 2) after all samples are added, the program automatically finishes coating, the flat plate and the coating rack are conveyed out of the equipment, and the program ends. In the operation process, the operation positions of the flat plate conveying cabin (reference numeral 19), the cabin cover (reference numeral 23), the support frame, the flat plate fixing disc (reference numeral 21) and the coating frame conveying belt need to be accurately controlled, corresponding parts are provided with position switches, and the position switches of all parts realize the position control of the corresponding parts.
The invention also designs a set of push rod control system which is positioned at one side of the flat plate and is opposite to the position of the flat plate outlet (reference numeral 10), when the flat plate returns to the initial position after coating, the lower flat plate shell is supported to the position of the flat plate outlet (reference numeral 10) by the supporting rod and is combined with the upper cover, at the moment, the push rod is started, so that the flat plate is ejected out from the plate outlet, and the upper and lower flat plate shells are well covered, thereby avoiding pollution.
The invention designs an intermediate platform, which is positioned about 25mm below a flat plate inlet (reference numeral 5) of a host machine, so that the height of the upper surface of the platform is not lower than the height of the lower edge of a flat plate outlet (reference numeral 10), the platform is provided with a round hole, the diameter of the round hole is larger than the outer diameter of a lower flat plate shell and smaller than the outer diameter of an upper flat plate shell, and the round hole is concentric with the flat plate inlet (reference numeral 5) of the host machine. After entering the apparatus through the inlet (reference numeral 5), the plate falls on the platform, as the plate moves further downward, the upper shell of the plate is stopped on the platform, so that the upper shell and the lower shell can be separated, and after coating is completed, the lower shell returns to the position to be combined with the upper shell, and then the plate is sent out of the apparatus through the outlet (reference numeral 10).
The invention also designs a retractable cover plate (reference numeral 8) above the sample adding port (reference numeral 7), when the operation is started, the cover plate (reference numeral 8) is pulled out, the special coating rack collecting bag is hung on the cover plate (reference numeral 8), and the coating rack automatically falls into the mobile phone credit to avoid polluting the environment.
The invention designs a foot switch (reference numeral 3) device which can replace a completion button and is convenient for a user to operate.
The automatic flat plate coating equipment designed by the invention has the advantages of simple operation, automatic disinfection function and automatic shutdown function. The user opens the power key, prepares to accomplish, presses and stops (reference numeral 2) button, and equipment begins to operate, after hearing the prompt tone, adds 1ml sample that awaits measuring to sample addition port (reference numeral 7), presses and accomplishes key (reference numeral 4), and all operations are accomplished automatically to equipment, hear the prompt tone again, and the sample is added again, circulates in proper order. After all the operations are finished, a start-stop key (reference numeral 2) is pressed, and after the equipment runs, a disinfection program is started, and the equipment is automatically shut down. The average time for coating one flat plate is only 3 to 4 seconds on average, so that the working efficiency is greatly improved. The equipment is also provided with an ozone disinfection system, all the operations are performed in a relatively sealed environment, the pollution of microorganisms can be avoided to the greatest extent, the operation is performed by one key, and after the disinfection is completed, the system automatically turns off the power supply without waiting.
Detailed Description
1 mounting of the plates
Placing the well-packed flat plates (10 bags) on an operation table, inverting, keeping the cover downward, keeping one surface of the culture medium upward, and placing the flat plates orderly to avoid toppling; the bag is cut open along the seal, the plate carrier chassis (reference numeral 14) is held in hand, and the plate carrier is inserted into the bag while the plate is loaded into the plate in the chamber. After the packaging bag is inserted to the bottom, a finger is placed in the middle of the bottom plate, the other hand holds the packaging bag and the lower end of the loading cabin or the fixed rod (reference numeral 12), the packaging bag and the lower end of the fixed rod are inclined at a certain angle, the finger is pressed onto the bottom flat plate, the flat plate loading frame is turned over, and the flat plate loading frame is placed on the host (reference numeral 31). The plate carrier is pushed into the plate entrance (reference numeral 5) of the host (reference numeral 31).
2. Coating rack mounting
The coating rack (10 bags in each bag) with intact inner package is laid flat and put into a loading groove of the coating rack, attention is paid to ensure that the lower end of the coating rack faces to the left towards a right positioning arm, the coating rack is cut off along a right sealing position by scissors, a base is loaded on the coating rack by holding, a rack fixing rod (reference numeral 17) is inserted into a packaging bag, the top end of the fixing rod (reference numeral 17) is inserted into a gap between the coating rack and the bottom of the fixing groove, the coating rack loading rack is pushed leftwards by the right hand, a packaging bag of the coating rack is held by the left hand, the coating rack positioning arm is fixed between the fixing rods (reference numeral 17), the coating rack is completely loaded in a cabin, and meanwhile, the packaging bag is sleeved outside the coating rack loading rack to play a. The lower opening of the bottom plate of the coating rack loading frame is blocked by hand, the base of the coating rack loading frame is downward and is placed on a main machine (reference numeral 31). The coating rack carrier is pushed into the host computer (reference numeral 31) into the coating rack entrance (reference numeral 6).
3. The system starts to operate
When a button of a start-stop (reference numeral 2) is pressed, a support (reference numeral 25) in the flat plate conveying cabin (reference numeral 19) descends by about 15mm, the upper shell of the flat plate is left on a platform at the position of a flat plate outlet (reference numeral 10), and the lower shell of the flat plate descends into the flat plate conveying cabin (reference numeral 19) along with the flat plate support frame.
The coating rack locking device is automatically switched on, the coating rack falls on the coating rack conveyor belt, the coating rack is conveyed forwards by 150mm, and the coating rack is stopped at the coating position.
The flat plate transport chamber (reference numeral 19) moves 150mm as a whole, reaches the coating position, and stops.
The plate holder continues to move 20mm downward, raising the hatch (23) by about 15mm, bringing the plate medium and the spreader into contact.
The device sends out a prompt tone to indicate that the preparation is finished and the sample can be added.
The user adds 1ml of sample from the sample addition port (reference numeral 7), and the sample flows into the coating arm slot along the sample addition port, and then presses the completion key (reference numeral 4) or depresses the foot switch (reference numeral 3).
The flat fixed disk (reference numeral 21) rotates for 2-3 circles. So that the sample in the coating arm groove is uniformly coated on the plate culture medium.
The plate support moves up 20mm and the hatch (reference numeral 23) is lowered.
The coating-stand conveyor belt continues to run forward for 2 seconds and the coating stand is conveyed out of the coating-stand outlet (reference numeral 9).
The plate conveying cabin (reference numeral 19) returns to the initial position, the plate supporting frame continues to move upwards for 20mm, the lower shell of the plate is supported to the position of the plate outlet (reference numeral 10), and the upper shell and the lower shell of the plate are combined together.
The electric push rod is activated to push the coated plate out to the plate outlet (reference numeral 10).
And taking out the flat plate, automatically entering the next hiking program by the equipment, and repeatedly executing the steps.
If the user finishes making one sample and does not make the next sample, after the user finishes adding the sample, the start-stop key (pop-up) is pressed again (reference numeral 2), the device finishes coating automatically, and the coating rack and the flat plate are conveyed out of the device.
The apparatus automatically performs a disinfection program. After the disinfection is finished, the power supply is automatically turned off.
The device is used for coating microorganisms, 10 samples are coated, the total time is less than 2 minutes, and the efficiency is improved by more than 5 times compared with the manual coating efficiency. The coating is uniform, and the coating area accounts for more than 90% of the whole plate area. The coating uniformity is higher than manual coating. It is also very convenient to install the plate and the coating stand, for a total time not exceeding 1 minute.