CN112961012A - Preparation method of microbial drip irrigation fertilizer - Google Patents
Preparation method of microbial drip irrigation fertilizer Download PDFInfo
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- CN112961012A CN112961012A CN202110255753.7A CN202110255753A CN112961012A CN 112961012 A CN112961012 A CN 112961012A CN 202110255753 A CN202110255753 A CN 202110255753A CN 112961012 A CN112961012 A CN 112961012A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 60
- 238000003973 irrigation Methods 0.000 title claims abstract description 46
- 230000002262 irrigation Effects 0.000 title claims abstract description 46
- 230000000813 microbial effect Effects 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims abstract description 42
- 238000000855 fermentation Methods 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 34
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 34
- 239000010806 kitchen waste Substances 0.000 claims abstract description 34
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 67
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 239000011591 potassium Substances 0.000 claims description 12
- 229910052700 potassium Inorganic materials 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 9
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 9
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 9
- 235000005822 corn Nutrition 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 241001052560 Thallis Species 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 description 78
- 241000219146 Gossypium Species 0.000 description 78
- 238000012360 testing method Methods 0.000 description 48
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 229910017053 inorganic salt Inorganic materials 0.000 description 12
- 238000009331 sowing Methods 0.000 description 10
- 244000005700 microbiome Species 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 230000004720 fertilization Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000010902 straw Substances 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000002689 soil Substances 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/70—Controlling the treatment in response to process parameters
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
The invention discloses a preparation method of a microbial drip irrigation fertilizer, which comprises the steps of inoculating bacillus subtilis and kitchen waste bacteria into a bacillus subtilis culture medium and a kitchen waste bacteria culture medium for activated culture, adding the bacillus subtilis culture medium and the kitchen waste bacteria culture medium for enlarged culture, and finally adding the bacillus subtilis culture medium and the kitchen waste bacteria culture medium into a prepared fertilizer for secondary fermentation culture to obtain a fertilizer finished product.
Description
Technical Field
The invention relates to a preparation method of a microbial drip irrigation fertilizer.
Background
China is a big agricultural country and is also a main cotton production area all over the world, and the cotton production areas of China mainly comprise Jianghuai plain, Jianghan plain, Nanjiang cotton area, North China plain, North Shandong plain, North Henan plain, and Yangtze river downstream coastal plain. Xinjiang is one of the most important cotton producing areas I pass through.
With the development of the drip irrigation technology, the drip irrigation planting is basically realized in the Xinjiang cotton planting area, the drip irrigation is the most effective water-saving irrigation mode in the drought and water-deficient area, and the water utilization rate can be 95%. In addition, the mineral elements required by the crops can be dripped to the crops along with water in the irrigation process. Therefore, a drip irrigation fertilizer with high cost performance and good using effect is needed.
Therefore, the applicant develops a drip irrigation fertilizer which utilizes microbial fermentation and is suitable for cotton planting operation.
Disclosure of Invention
The invention discloses a drip irrigation fertilizer which is fermented by microorganisms and is suitable for cotton planting operation.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a preparation method of a microbial drip irrigation fertilizer is characterized by comprising the following steps: comprises the following steps:
selecting a culture medium, namely selecting a bacillus subtilis culture medium and a kitchen waste culture medium;
secondly, inoculation and culture: taking bacillus subtilis and kitchen waste bacteria, respectively inoculating the bacillus subtilis and the kitchen waste bacteria after sterilization to a bacillus subtilis culture medium and a kitchen waste bacteria culture medium, placing the inoculated bacillus subtilis and the kitchen waste bacteria culture medium on a shaking table in an aseptic culture room for shake culture, controlling the temperature of the culture room at 30-36 ℃, culturing for 36 hours, taking down the shaking table, and keeping the content of viable bacteria after culture to be more than 40 hundred million/ml;
③ enlarged culture: screening out high-quality strains with a large number of thalli from the strains obtained by the step two, then respectively putting the screened high-quality strains into fermentation tanks containing a high-temperature sterilized bacillus subtilis expanded culture medium and a kitchen waste bacteria expanded culture medium, and carrying out fermentation culture for 36 hours, wherein the fermentation temperature is controlled to be 30-36 ℃, and the pH is not less than 5.5 and not more than 6.8; in the fermentation process, oxygen is supplied in the whole process, and the stirring system is started and stirred for 0.5h at intervals of 2 h; after the culture is finished (the number of viable bacteria is more than or equal to 20 hundred million/ml), cooling the temperature of the bacterial liquid in the fermentation tank to 10 ℃, and storing for later use;
fourthly, preparing a fertilizer: dissolving 50KG edible brown sugar, 60KG edible corn flour, 30KG urea, 100KG potassium fulvate raw powder and 60KG water in 700KG liquid amino acid, stirring and dissolving uniformly, and then putting into a fermentation tank for sterilization treatment;
fifth, secondary fermentation: cooling the solution dissolved in the fourth step to 30 ℃, then inoculating the bacterial liquid obtained in the third step, performing secondary fermentation at the fermentation temperature of 30-36 ℃, the pH value of not less than 5.5 and not more than 6.8, wherein the fermentation time is 48 hours, and sampling and detecting in a laboratory to reach the technical requirements, thereby obtaining the finished fertilizer;
2L of the amount of the Bacillus subtilis solution and 1L of the amount of the kitchen waste solution. The technical requirement of finished fertilizer products after fermentation requires that the detection indexes are that the number of viable bacillus subtilis is more than or equal to 2 hundred million/ml and the number of viable kitchen waste bacteria is more than or equal to 1 hundred million/ml.
The bacillus subtilis culture medium in the step I is as follows: 150g of glucose (carbon source), 10g of corn steep liquor (nutrient), (NH)4)2SO43g (Nitrogen source), MgSO42g (inorganic salt), MnSO40.2g (inorganic salt), 1L water, pH 7.0-7.2.
The kitchen waste culture medium in the step I is as follows: potato powder (200 g of nutrient), glucose (20 g of carbon source), (NH)4)2SO41.5g (nitrogen source), K2HP040.1g (inorganic salt) water 1L, natural PH.
The bacillus subtilis expanded culture medium in the third step is as follows: brown sugar (100 KG) and corn flour (50 KG), (NH) as nutrients4)2SO43KG (nitrogen source), MgSO42KG (inorganic salt), MnSO40.2KG (inorganic salt), 1000L water, pH 7.0-7.2.
The kitchen waste bacteria expanding culture medium comprises the following steps: potato powder (100 KG), soybean powder (30 KG), brown sugar (30 KG), (NH) carbon source4)2SO41.5KG (nitrogen source), K2HP040.15KG (inorganic salt), 1000L water, natural pH.
The product can be used in the whole growth cycle, can quickly break seed dormancy, and promote seed rooting and germination; the effects of loosening soil, breaking hardened soil and promoting root growth and development can be achieved, and meanwhile, strong seedlings can be grown early; the disease of cotton withering and verticillium wilt can be prevented and controlled; can also improve the saline-alkali property and adjust the pH value of the soil.
Drawings
FIG. 1 is a schematic diagram of the preparation process of the present invention.
Detailed Description
The present invention is not limited by the following examples, and specific embodiments may be determined according to the technical solutions and practical situations of the present invention.
The invention is further described with reference to the following examples and figures:
example 1: the embodiment 1 of the invention discloses a preparation method of a microbial drip irrigation fertilizer, which is characterized by comprising the following steps: comprises the following steps:
selecting a culture medium, namely selecting a bacillus subtilis culture medium and a kitchen waste culture medium;
secondly, inoculation and culture: taking bacillus subtilis and kitchen waste bacteria, respectively inoculating the bacillus subtilis and the kitchen waste bacteria after sterilization to a bacillus subtilis culture medium and a kitchen waste bacteria culture medium, placing the inoculated bacillus subtilis and the kitchen waste bacteria culture medium on a shaking table in an aseptic culture room for shake culture, controlling the temperature of the culture room at 30-36 ℃, culturing for 36 hours, taking down the shaking table, and keeping the content of viable bacteria after culture to be more than 40 hundred million/ml;
③ enlarged culture: screening out high-quality strains with a large number of thalli from the strains obtained by the step two, then respectively putting the screened high-quality strains into fermentation tanks containing a high-temperature sterilized bacillus subtilis expanded culture medium and a kitchen waste bacteria expanded culture medium, and carrying out fermentation culture for 36 hours, wherein the fermentation temperature is controlled to be 30-36 ℃, and the pH is not less than 5.5 and not more than 6.8; in the fermentation process, oxygen is supplied in the whole process, and the stirring system is started and stirred for 0.5h at intervals of 2 h; after the culture is finished (the number of viable bacteria is more than or equal to 20 hundred million/ml), cooling the temperature of the bacterial liquid in the fermentation tank to 10 ℃, and storing for later use;
fourthly, preparing a fertilizer: dissolving 50KG edible brown sugar, 60KG edible corn flour, 30KG urea, 100KG potassium fulvate raw powder and 60KG water in 700KG liquid amino acid, stirring and dissolving uniformly, and then putting into a fermentation tank for sterilization treatment;
fifth, secondary fermentation: cooling the solution dissolved in the fourth step to 30 ℃, then inoculating the bacterial liquid obtained in the third step, performing secondary fermentation at the fermentation temperature of 30-36 ℃, the pH value of not less than 5.5 and not more than 6.8, wherein the fermentation time is 48 hours, and sampling and detecting in a laboratory to reach the technical requirements, thereby obtaining the finished fertilizer;
2L of the amount of the Bacillus subtilis solution and 1L of the amount of the kitchen waste solution. The technical requirement of finished fertilizer products after fermentation requires that the detection indexes are that the number of viable bacillus subtilis is more than or equal to 2 hundred million/ml and the number of viable kitchen waste bacteria is more than or equal to 1 hundred million/ml.
The bacillus subtilis culture medium in the step I is as follows: 150g of glucose (carbon source), 10g of corn steep liquor (nutrient), (NH)4)2SO43g (Nitrogen source), MgSO42g (inorganic salt), MnSO40.2g (inorganic salt), 1L water, pH 7.0-7.2.
The kitchen waste culture medium in the step I is as follows: potato powder (200 g of nutrient), glucose (20 g of carbon source), (NH)4)2SO41.5g (nitrogen source), K2HP040.1g (inorganic salt) water 1L, natural PH.
The bacillus subtilis expanded culture medium in the third step is as follows: brown sugar (100 KG) and corn flour (50 KG), (NH) as nutrients4)2SO43KG (nitrogen source), MgSO42KG (inorganic salt), MnSO40.2KG (inorganic salt), 1000L water, pH 7.0-7.2.
The kitchen waste bacteria expanding culture medium comprises the following steps: potato powder (100 KG), soybean powder (30 KG), brown sugar (30 KG), (NH) carbon source4)2SO41.5KG (nitrogen source), K2HP040.15KG (inorganic salt), 1000L water, natural pH.
Example 2: example 2 using the fertilizer prepared as in example 1, the field data is as follows:
firstly, test purpose, through tests, the cotton emergence time and the cotton emergence rate are measured under the same plot, the same sowing period, the same crop and the same water dripping time.
II, test site and area: the stone river 121 cliques 20 blossoms of cotton fields. The previous crop is cotton and loam. The straw is turned over after returning to the field, and deep fertilization is not applied.
Test area: 100 acres, wherein the drip irrigation fertilizer area of the special microorganisms for cotton drip seedling emergence prepared in the example 1 is 30 acres; the area of the applied potassium humate is 30 mu; contrast 40 mu
Thirdly, testing materials: microbial drip irrigation fertilizer special for cotton seedling emergence prepared in example 1
Fourthly, test design: when seedling water is dropped after cotton sowing, the water drop per mu is about 15-20m3. Test treatment I: 5kg of the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is applied to each mu of cotton seedlings along with water drops, and the fertilizer is dripped along with water drops 1 hour before water cutAnd (6) adding. And (2) test treatment II: 5kg of potassium humate is applied to each mu of land along with water drops, and the potassium humate is dropped along with the water 1 hour before the water is cut off. And (3) test treatment III: drip applying clear water (contrast)
Field survey form:
and (4) conclusion: 1. the data in the table show that the special microbial agent for cotton drip seedling emergence prepared in example 1 has seedling emergence 2-3 days earlier than that of the cotton drip seedling emergence treatment and 3-4 days earlier than that of the control.
2. The data show that the emergence rate of the special microbial drip irrigation fertilizer for cotton drip emergence, which is prepared in the application example 1, is improved by 3% compared with the secondary emergence rate of the treatment and is improved by 5% compared with the control emergence rate.
3. The test data show that the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is dripped with water during cotton seedling emergence, and the effect is obvious compared with that of cotton seedling emergence earlier by 2-4 days. Can achieve the aims of early seedling, complete seedling, uniform seedling, even seedling and strong seedling.
Example 3: example 3 Using the fertilizer prepared as in example 1, the field data is as follows:
firstly, test purpose, through tests, the cotton emergence time and the cotton emergence rate are measured under the same plot, the same sowing period, the same crop and the same water dripping time.
II, test site and area: the stone river 136 gathers cotton fields of Jiu Lian Zhou. The previous crop is cotton and loam. The straw is turned over after returning to the field, and deep fertilization is not applied.
Test area: 160 mu, wherein the drip irrigation fertilizer area of the special microorganisms for cotton drip seedling emergence prepared in the example 1 is 50 mu; the area of the applied potassium humate is 50 mu; control 60 mu.
Thirdly, testing materials: microbial drip irrigation fertilizer special for cotton seedling emergence prepared in example 1
Fourthly, test design: when seedling water is dropped after cotton sowing, the water drop per mu is about 15-20m3. Test treatment I: 5kg of the special microbial drip irrigation fertilizer for cotton drip seedling emergence prepared in the example 1 is applied to each mu of cotton along with water drops, and the fertilizer is 1 hour before water supply is cut offIt is dripped with water. And (2) test treatment II: 3kg of potassium humate is applied to each mu of land along with water drops, and the potassium humate is dropped along with the water 1 hour before the water is cut off. And (3) test treatment III: drip applying clear water (contrast)
Field survey form:
and (4) conclusion: 1. the data in the table show that the special microbial agent for cotton drip seedling emergence prepared in example 1 is applied for 3 days earlier than the second treatment and 4 days earlier than the control.
2. The data show that the emergence rate of the special microbial drip irrigation fertilizer for cotton drip emergence, which is prepared in the application example 1, is improved by 6% compared with the secondary emergence rate of the treatment, and is improved by 10% compared with the control emergence rate.
3. The test data show that the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is dripped with water during cotton seedling emergence, and the effect is obvious compared with that of cotton seedling emergence earlier by 3-4 days. Can achieve the aims of early seedling, complete seedling, uniform seedling, even seedling and strong seedling.
Example 4: example 4 using the fertilizer prepared as in example 1, the field data is as follows:
firstly, test purpose, through tests, the cotton emergence time and the cotton emergence rate are measured under the same plot, the same sowing period, the same crop and the same water dripping time.
II, test site and area: the stone river 149 Tu Shi Lian Cheng Ming Cotton field. The previous crops are cotton and sandy loam. The straw is turned over after returning to the field, and deep fertilization is not applied.
Test area: 150 acre, wherein the drip irrigation fertilizer area of the special microorganisms for cotton drip seedling emergence prepared in the example 1 is 50 acres; control 100 mu.
Thirdly, testing materials: the microbial drip irrigation fertilizer special for cotton seedling emergence prepared in the embodiment 1.
Fourthly, test design: when seedling water is dropped after cotton sowing, the water drop per mu is about 15-20m3. Test treatment I: 5kg of the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is applied to each mu of cotton seedlings along with water drops, and the fertilizer is dripped along with water drops 1 hour before water cutAnd (6) adding. And (2) test treatment II: drip applying clear water (contrast)
Field survey form:
and (4) conclusion: 1. the data in the table show that the special microbial agent for cotton drip seedling emergence prepared in example 1 is applied for 2-3 days earlier than the control.
2. The data show that the seedling emergence rate of the special microorganism drip irrigation fertilizer for cotton seedling emergence prepared in the application example 1 is improved by 5 percent compared with the average seedling emergence rate of a control.
3. The test data show that the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is dripped with water during cotton seedling emergence, and the effect is obvious compared with that of cotton seedling emergence earlier by 2-3 days. Can achieve the aims of early seedling, complete seedling, uniform seedling, even seedling and strong seedling.
Example 5: example 5 Using the fertilizer prepared as in example 1, the field data is as follows:
firstly, test purpose, through tests, the cotton emergence time and the cotton emergence rate are measured under the same plot, the same sowing period, the same crop and the same water dripping time.
II, test site and area: the rocky river 150 gathers cotton fields of the kind of Thailand. The previous crops are cotton and sandy loam. The straw is turned over after returning to the field, and deep fertilization is not applied.
Test area: 180 mu, wherein the drip irrigation fertilizer area of the special microorganisms for cotton drip seedling emergence prepared in the example 1 is 50 mu; the area of the applied potassium humate is 50 mu; control 80 mu.
Thirdly, testing materials: the microbial drip irrigation fertilizer special for cotton seedling emergence prepared in the embodiment 1.
Fourthly, test design: when seedling water is dropped after cotton sowing, the water drop per mu is about 15-20m3. Test treatment I: 5kg of the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is applied to each mu of cotton seedlings along with water drops, and the special microbial drip irrigation fertilizer is dropped along with water 1 hour before water is cut off. And (2) test treatment II: 5kg of potassium humate is applied to each mu of land along with water drops, and the potassium humate is dropped along with the water 1 hour before the water is cut off. And (3) test treatment III: dripping clear water (Control)
Field survey form:
and (4) conclusion: 1. the data in the table show that the special microbial agent for cotton drip seedling emergence prepared in example 1 is applied for 3 days earlier than the second treatment and 4 days earlier than the control.
2. The data show that the emergence rate of the special microbial drip irrigation fertilizer for cotton drip emergence, which is prepared in the application example 1, is improved by 3% compared with the secondary emergence rate of the treatment and improved by 6% compared with the control emergence rate.
3. The test data show that the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is dripped with water during cotton seedling emergence, and the effect is obvious compared with that of cotton seedling emergence earlier by 2-4 days. Can achieve the aims of early seedling, complete seedling, uniform seedling, even seedling and strong seedling.
Example 6: example 6 using the fertilizer prepared as in example 1, the field data is as follows:
firstly, test purpose, through tests, the cotton emergence time and the cotton emergence rate are measured under the same plot, the same sowing period, the same crop and the same water dripping time.
II, test site and area: cotton fields of six households in the county of manass, mana, township, maja, dynasty, wealth. The previous crop is cotton and loam. The straw is turned over after returning to the field, and deep fertilization is not applied.
Test area: 200 acres, wherein the drip irrigation fertilizer area of the special microorganisms for cotton drip seedling emergence prepared in the example 1 is 40 acres; and the contrast is 120 mu.
Thirdly, testing materials: microbial drip irrigation fertilizer special for cotton seedling emergence prepared in example 1
Fourthly, test design: when seedling water is dropped after cotton sowing, the water drop per mu is about 15-20m3. Test treatment I: 5kg of the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is applied to each mu of cotton seedlings along with water drops, and the special microbial drip irrigation fertilizer is dropped along with water 1 hour before water is cut off. And (2) test treatment II: drip applying clear water (contrast)
Field survey form:
and (4) conclusion: 1. the data in the table show that the emergence of the special microbial agent for cotton drip emergence prepared in example 1 can be found for 2-3 days compared with the control.
2. The data show that the seedling emergence rate of the special microorganism drip irrigation fertilizer for cotton seedling emergence prepared in the application example 1 is improved by 7 percent compared with the seedling emergence rate of a control.
3. The test data show that the special microbial drip irrigation fertilizer for cotton seedling emergence prepared in the example 1 is dripped with water during cotton seedling emergence, and the effect is obvious compared with that of cotton seedling emergence earlier by 2-3 days. Can achieve the aims of early seedling, complete seedling, uniform seedling, even seedling and strong seedling.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the inventive concept of the present invention, and these changes and modifications are all within the scope of the present invention.
Claims (5)
1. A preparation method of a microbial drip irrigation fertilizer is characterized by comprising the following steps: comprises the following steps:
selecting a culture medium, namely selecting a bacillus subtilis culture medium and a kitchen waste culture medium;
secondly, inoculation and culture: taking bacillus subtilis and kitchen waste bacteria, respectively inoculating the bacillus subtilis and the kitchen waste bacteria after sterilization to a bacillus subtilis culture medium and a kitchen waste bacteria culture medium, placing the inoculated bacillus subtilis and the kitchen waste bacteria culture medium on a shaking table in an aseptic culture room for shake culture, controlling the temperature of the culture room at 30-36 ℃, culturing for 36 hours, taking down the shaking table, and keeping the content of viable bacteria after culture to be more than 40 hundred million/ml;
③ enlarged culture: screening out high-quality strains with a large number of thalli from the strains obtained by the step two, then respectively putting the screened high-quality strains into fermentation tanks containing a high-temperature sterilized bacillus subtilis expanded culture medium and a kitchen waste bacteria expanded culture medium, and carrying out fermentation culture for 36 hours, wherein the fermentation temperature is controlled to be 30-36 ℃, and the pH is not less than 5.5 and not more than 6.8; in the fermentation process, oxygen is supplied in the whole process, and the stirring system is started and stirred for 0.5h at intervals of 2 h; after the culture is finished (the number of viable bacteria is more than or equal to 20 hundred million/ml), cooling the temperature of the bacterial liquid in the fermentation tank to 10 ℃, and storing for later use;
fourthly, preparing a fertilizer: dissolving 50KG edible brown sugar, 60KG edible corn flour, 30KG urea, 100KG potassium fulvate raw powder and 60KG water in 700KG liquid amino acid, stirring and dissolving uniformly, and then putting into a fermentation tank for sterilization treatment;
fifth, secondary fermentation: cooling the solution dissolved in the fourth step to 30 ℃, then inoculating the bacterial liquid obtained in the third step, performing secondary fermentation at the fermentation temperature of 30-36 ℃, the pH value of not less than 5.5 and not more than 6.8, wherein the fermentation time is 48 hours, and sampling and detecting in a laboratory to reach the technical requirements, thereby obtaining the finished fertilizer;
2L of the amount of the Bacillus subtilis solution and 1L of the amount of the kitchen waste solution. The technical requirement of finished fertilizer products after fermentation requires that the detection indexes are that the number of viable bacillus subtilis is more than or equal to 2 hundred million/ml and the number of viable kitchen waste bacteria is more than or equal to 1 hundred million/ml.
2. The method for preparing a microbial drip irrigation fertilizer according to claim 1, wherein the method comprises the following steps: the bacillus subtilis culture medium in the step I is as follows: 150g of glucose, 10g of corn steep liquor and (NH)4)2SO4＝3g，MgSO4＝2g，MnSO40.2g, 1L of water and 7.0-7.2 of pH.
3. The method for preparing a microbial drip irrigation fertilizer according to claim 1, wherein the method comprises the following steps: the kitchen waste culture medium in the step I is as follows: 200g of potato powder, 20g of glucose, (NH)4)2SO4＝1.5g，K2HP04Water 0.1 g-1L, natural PH.
4. The method for preparing a microbial drip irrigation fertilizer according to claim 1, wherein the method comprises the following steps: step three:
the bacillus subtilis expanded culture medium comprises the following components: 100KG brown sugar, 50KG corn flour, (NH)4)2SO4＝3KG，MgSO4＝2KG，MnSO40.2KG, 1000L water, 7.0-7.2 pH.
5. The method for preparing a microbial drip irrigation fertilizer according to claim 1, wherein the method comprises the following steps: step three:
the kitchen waste bacteria expanding culture medium comprises the following components: potato powder (100 KG), soybean powder (30 KG), brown sugar (30 KG), (NH)4)2SO4＝1.5KG，K2HP040.15KG, 1000L of water, natural pH.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113956095A (en) * | 2021-11-15 | 2022-01-21 | 成都华宏生物科技有限公司 | Nutritional biological water-retaining agent composition, and preparation method and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| CN101698627A (en) * | 2009-11-25 | 2010-04-28 | 北京理工大学 | Multifunctional microbial fertilizer and preparation method thereof |
| CN102258059A (en) * | 2011-07-13 | 2011-11-30 | 山东玉成生化农药有限公司 | Method for preparing ralstonia solanacearum-resistant bacillus subtilis microbial inoculum |
| CN102432362A (en) * | 2011-09-05 | 2012-05-02 | 麻林涛 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
| CN102888356A (en) * | 2012-09-03 | 2013-01-23 | 新疆农业科学院土壤肥料与农业节水研究所 | Bacillus subtilis, microbial fertilizer prepared by using bacillus subtilis, method for preparing microbial fertilizer by using bacillus subtilis, and application of microbial fertilizer |
| CN103787697A (en) * | 2013-12-31 | 2014-05-14 | 成都恒润高新科技有限公司 | Processing method of kitchen wastes and application |
| CN105418191A (en) * | 2015-11-13 | 2016-03-23 | 山东郁茏生物科技有限公司 | Microbial fertilizer prepared from kitchen garbage |
| CN105585381A (en) * | 2015-12-23 | 2016-05-18 | 成都新柯力化工科技有限公司 | Microbial water-soluble fertilizer and preparation method thereof |
| CN105646104A (en) * | 2015-12-30 | 2016-06-08 | 湖南泰谷生物肥料有限公司 | Fully water-soluble composite biological bacterial fertilizer as well as preparation method and application thereof |
| CN105819943A (en) * | 2016-03-01 | 2016-08-03 | 深圳市芭田生态工程股份有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN108484278A (en) * | 2018-05-28 | 2018-09-04 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | A kind of high activity bacillus subtilis compound amino acid liquid fertilizer being exclusively used in trickle irrigation |
-
2021
- 2021-03-09 CN CN202110255753.7A patent/CN112961012A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| CN101698627A (en) * | 2009-11-25 | 2010-04-28 | 北京理工大学 | Multifunctional microbial fertilizer and preparation method thereof |
| CN102258059A (en) * | 2011-07-13 | 2011-11-30 | 山东玉成生化农药有限公司 | Method for preparing ralstonia solanacearum-resistant bacillus subtilis microbial inoculum |
| CN102432362A (en) * | 2011-09-05 | 2012-05-02 | 麻林涛 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
| CN102888356A (en) * | 2012-09-03 | 2013-01-23 | 新疆农业科学院土壤肥料与农业节水研究所 | Bacillus subtilis, microbial fertilizer prepared by using bacillus subtilis, method for preparing microbial fertilizer by using bacillus subtilis, and application of microbial fertilizer |
| CN103787697A (en) * | 2013-12-31 | 2014-05-14 | 成都恒润高新科技有限公司 | Processing method of kitchen wastes and application |
| CN105418191A (en) * | 2015-11-13 | 2016-03-23 | 山东郁茏生物科技有限公司 | Microbial fertilizer prepared from kitchen garbage |
| CN105585381A (en) * | 2015-12-23 | 2016-05-18 | 成都新柯力化工科技有限公司 | Microbial water-soluble fertilizer and preparation method thereof |
| CN105646104A (en) * | 2015-12-30 | 2016-06-08 | 湖南泰谷生物肥料有限公司 | Fully water-soluble composite biological bacterial fertilizer as well as preparation method and application thereof |
| CN105819943A (en) * | 2016-03-01 | 2016-08-03 | 深圳市芭田生态工程股份有限公司 | Microbial organic fertilizer and preparation method thereof |
| CN108484278A (en) * | 2018-05-28 | 2018-09-04 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | A kind of high activity bacillus subtilis compound amino acid liquid fertilizer being exclusively used in trickle irrigation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113956095A (en) * | 2021-11-15 | 2022-01-21 | 成都华宏生物科技有限公司 | Nutritional biological water-retaining agent composition, and preparation method and application thereof |
| CN113956095B (en) * | 2021-11-15 | 2023-09-01 | 成都华宏生物科技有限公司 | Nutritional biological water-retaining agent composition and preparation method and application thereof |
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Application publication date: 20210615 |