CN112945836B - Automatic throughput device for clip-on cell counting chamber - Google Patents
Automatic throughput device for clip-on cell counting chamber Download PDFInfo
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- CN112945836B CN112945836B CN202110477757.XA CN202110477757A CN112945836B CN 112945836 B CN112945836 B CN 112945836B CN 202110477757 A CN202110477757 A CN 202110477757A CN 112945836 B CN112945836 B CN 112945836B
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- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000033001 locomotion Effects 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 22
- 230000005540 biological transmission Effects 0.000 description 2
- 210000005056 cell body Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003466 welding Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
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Abstract
本发明公开了卡扣夹持式细胞计数板自动吞吐装置，包括承片平台、弹簧合页、移动组件；承片平台的顶面中心沿长度方向开设有凹槽；凹槽的进口插入有厚度小于凹槽深度的计数板；移动组件与弹簧合页的一半合页上固定；弹簧合页另一半合页的底面固定有端部带有楔块的卡扣；卡扣端部楔块的斜下端朝向弹簧合页的中部设置；卡扣端部楔块的外端搭在挡块上；挡块设置在计数板的上方；计数板朝向移动组件的端部开设有与卡扣相互卡接的卡孔；移动组件通过驱动组件带动前进；本发明，通过设置弹簧合页及连接在弹簧合页底部的卡扣，由弹簧合页弹性作用抵消移动组件由于制造精度差导致的运动不稳定，减少影响焦距的因素。
The invention discloses a buckle clamping type cell counting plate automatic throughput device, comprising a plate holding platform, a spring hinge and a moving component; a groove is provided at the center of the top surface of the plate holding platform along the length direction; a counting plate with a thickness less than the depth of the groove is inserted into the inlet of the groove; the moving component is fixed on one half of the spring hinge; a buckle with a wedge block at the end is fixed on the bottom surface of the other half of the spring hinge; the oblique lower end of the wedge block at the end of the buckle is arranged toward the middle of the spring hinge; the outer end of the wedge block at the end of the buckle is placed on the stop block; the stop block is arranged above the counting plate; a clamping hole that is mutually clamped with the buckle is provided at the end of the counting plate facing the moving component; the moving component is driven forward by the driving component; the invention, by arranging the spring hinge and the buckle connected to the bottom of the spring hinge, the elastic action of the spring hinge offsets the unstable movement of the moving component caused by poor manufacturing accuracy, thereby reducing the factors affecting the focal length.
Description
Technical Field
The invention relates to the technical field of medical machinery cell counting, in particular to an automatic throughput device of a clamping type cell counting plate.
Background
The existing cell counting plate is manually pushed into the appointed position of the sliding table bearing surface, the driving motor rotates to drive the motor gear to rotate, so that the sliding table with the rack moves forwards to enter the observation area, after the observation area of the counting plate is photographed, the motor rotates reversely to drive the sliding table to retreat to the initial position, the counting plate is pulled out, and the whole work is completed.
The utility model discloses a cell counting plate, including the box body, the lower extreme welding of box body has the cushion, the upper end joint of cushion has the cell body, be provided with the sponge piece in the cell body, the standing groove has been seted up to the sponge piece upper end, place the cell counting plate in the standing groove, the upper end of cell counting plate is seted up flutedly, the upper end contact of cell counting plate is connected with the coverslip, the upper end of box body articulates there is the lid, the filtration pore has been seted up to the upper end of lid, the lower extreme bonding of lid has the mount pad, the lower extreme of mount pad is provided with the spring, the lower extreme fixedly connected with pressure strip of spring, at the during operation, open the lid, place the cell counting plate in the standing groove, place the coverslip on the cell counting plate, cover, the lid passes through spacing hole and spacer pin cooperation is fixed, the lid is fixed to compress tightly sealed the pad, make the inside of box body be sealed, then the cell counting plate of pulling advances, but this kind of mode cell counting plate, through manual mode pulling, not only convenient, and the stability is poor, the pulling, the lower extreme stability exists, the pulling is great, the section is adjusted in the complicated operation.
However, because the observation effect of the cell counting plate is influenced by the focal length between the lens and the counting plate, especially the throughput device with the electric sliding table, the main factors influencing the focal length of the lens are the manufacturing precision of the sliding table, the assembling precision of the sliding table, the manufacturing precision of the sliding table mounting plate, the assembling precision of the sliding table mounting plate and the assembling precision of the lens, the factors greatly increase the difficulty of processing parts, have high assembly requirements, and often have different observation definition between different hole sites of the same counting plate under the condition that the high requirements cannot be met, and frequent focusing is needed.
Disclosure of Invention
The invention aims to solve the technical problem of providing the automatic throughput device for the buckle clamping type cell counting plate, which is low in processing difficulty and low in assembly requirement.
The technical scheme is that the automatic handling device for the buckle clamping type cell counting plate comprises a piece bearing platform, a driving assembly, a counting plate, a spring hinge and a moving assembly, wherein a groove is formed in the center of the top surface of the piece bearing platform along the length direction, the counting plate with the thickness smaller than the depth of the groove is inserted into an inlet of the groove, the moving assembly is fixed on one half hinge of the spring hinge, a buckle with a wedge block at the end is fixed on the bottom surface of the other half hinge of the spring hinge, the inclined lower end of the wedge block at the end part of the buckle is arranged towards the middle part of the spring hinge, the outer end of the wedge block at the end part of the buckle is arranged on a stop block, the stop block is arranged above the counting plate, a clamping hole which is mutually clamped with the buckle is formed in the end part of the counting plate towards the moving assembly, and the moving assembly is driven to move forward by the driving assembly.
Through setting up spring hinge and connecting the buckle in spring hinge bottom, when step motor drives the counter plate and advances, offset the motion that removes the subassembly because the manufacturing accuracy is poor leads to by spring hinge's elasticity effect unstable, reduce the factor that influences the focus.
Preferably, side pressing plates are arranged on two sides of an inlet of the groove, the side pressing plates are fixed on the top surface of the wafer bearing platform, and the distance between the adjacent surfaces of the two side pressing plates is smaller than the width of an opening of the groove. Limiting the top of the counting plate and preventing the counting plate from separating from the top opening of the groove.
Preferably, the stop blocks cross the grooves, and the two end parts of the stop blocks are embedded into a gap between the side pressure plate and the carrying platform.
The driving assembly comprises a stepping motor, a motor gear and a rack, wherein the stepping motor is fixed at the bottom of the piece bearing platform, an output shaft is connected to the output end of the stepping motor in a transmission mode, the end portion of the output shaft vertically penetrates through the piece bearing platform and is fixedly sleeved with the motor gear, one side of the motor gear is meshed with the rack, the length direction of the rack is consistent with the length direction of the groove, one side of the rack with teeth faces away from the groove, and the back side of the rack is fixedly connected with the moving assembly.
The sliding guide comprises a sliding table and a sliding block, wherein the sliding table is fixed on the sheet bearing platform, the guiding direction of the sliding table is parallel to the length direction of the groove, the sliding table is connected with the sliding block in a sliding manner, and the sliding block is fixedly connected with the moving assembly.
The movable assembly comprises a movable plate, a semicircular column groove, a cylinder and a connecting boss, wherein the output end of the driving assembly is fixedly connected with the boss, the side part of the movable plate, which faces the boss, is fixed with the sliding block, the side wall of the movable plate, which faces the boss, is provided with a clamping groove with the length being arranged up and down, one side of the boss, which faces the movable plate, is fixed with the clamping column, and the clamping column is embedded in the clamping groove.
Preferably, the clamping column is a cylinder, and the clamping groove is a semicircular column groove with the inner diameter equal to the outer diameter of the cylinder.
Preferably, a camera lens is arranged above the mobile plate.
Preferably, the carrying platform and the sliding table are fixed through bolts.
Preferably, a plurality of observation areas are arranged on the counting plate.
Inserting a counting plate along a groove inlet, pushing the moving plate to move after the head end of the counting plate is propped against the moving plate, driving the spring hinge at the top to move forward by the moving plate, driving the moving plate to move forward by the connecting boss through the clamping action of a cylinder and a semi-cylinder, driving the counting plate to move forward by the moving plate after the buckle at the bottom of the other hinge is far away from the stop block, enabling the speed of the stepping motor to be constant, driving the counting plate to move stably after the slope at the bottom of the wedge block at the end of the buckle is completely separated from the top surface of the stop block, observing the cell condition on the counting plate, reversing the stepping motor under the elastic action of the spring hinge, driving the clamping hole at the front end of the counting plate by the buckle, driving the wedge block to move along the length direction of the groove, driving the connecting boss at the back side of the rack to move by the clamping action of the cylinder and the semi-cylinder, driving the counting plate to move forward by the connecting boss and the clamping action of the spring hinge and the clamping hole, driving the counting plate to move forward by the moving plate, driving the counting plate to move through the camera lens, observing the cell condition on the counting plate after the observation is completed, driving the stepping motor to rotate reversely, and driving the counting plate to move along the top surface of the stop block, after the counting plate is pushed by the cell condition on the observation plate, and the top surface of the plate, and the cell condition is separated from the top plate and the plate; by arranging the spring hinge and the buckle connected to the bottom of the spring hinge, when the stepping motor drives the counting plate to advance, the elastic action of the spring hinge counteracts the unstable movement of the sliding table, the sliding block, the rack and the moving plate caused by poor manufacturing precision, reduces factors influencing focal length, the counting plate moves in the groove, can improve the observing definition only by improving the precision of the groove, does not need to adjust the camera lens for many times, eliminates the influence of the assembly factors of the camera lens, and only needs to push the counting plate into the groove during operation, the stepping motor is started, and the operation is simple.
Compared with the prior art, the invention has the beneficial effects that:
A. by arranging the spring hinge and the buckle connected to the bottom of the spring hinge, when the stepping motor drives the counting plate to advance, the elastic action of the spring hinge counteracts the unstable movement of the moving assembly caused by poor manufacturing precision, and the factors influencing the focal length are reduced;
B. the counting plate is pushed into the groove, the clamping holes on the counting plate are buckled with the buckles into a whole, and the counting plate can be driven to move by starting the driving assembly, so that the operation is simple;
a side pressing plate is arranged to limit the top of the counting plate and prevent the counting plate from separating from the top opening of the groove;
the counting plate moves in the groove, so that the observation definition can be improved only by improving the precision of the groove, the camera lens is not required to be adjusted for many times, and the influence of the assembly factors of the camera lens is eliminated.
Drawings
FIG. 1 is a schematic perspective view of an automated throughput device for a buckle-clamped cell counting plate according to the present invention;
FIG. 2 is an enlarged view of area A of FIG. 1 in accordance with the present invention;
FIG. 3 is an enlarged view of area B of FIG. 1 in accordance with the present invention;
FIG. 4 is a schematic elevational view of the automatic throughput device for a snap-clamp cell counting plate according to the present invention;
FIG. 5 is a schematic view of section C-C of FIG. 4 in accordance with the present invention.
The drawing number is 1, the sheet bearing platform, 101, the groove, 2, the driving component, 201, the stepping motor, 202, the motor gear, 203, the rack, 3, the linear guide rail, 301, the sliding table, 302, the sliding block, 4, the side pressure plate, 5, the counting plate, 6, the spring hinge, 7, the moving component, 701, the moving plate, 702, the semicircular column groove, 703, the cylinder, 704, the connecting boss, 8, the buckle, 9 and the stop block.
Detailed Description
In order to facilitate the understanding of the technical scheme of the present invention by those skilled in the art, the technical scheme of the present invention will be further described with reference to the accompanying drawings.
The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1 and 2, the embodiment discloses an automatic throughput device for a buckle clamping type cell counting plate, which comprises a piece bearing platform 1, a driving assembly 2, a linear guide rail 3, a side pressing plate 4, a counting plate 5, a spring hinge 6 and a moving assembly 7.
The center of the top surface of the carrying platform 1 is provided with a groove 101 along the length direction. Side pressing plates 4 are arranged on two sides of the inlet of the groove 101. The side pressure plate 4 is fixed on the top surface of the wafer bearing platform 1 through bolts. And the distance between the adjacent surfaces of the two side pressure plates 4 is smaller than the opening width of the groove 101. The inlet of the groove 101 is inserted with a counting plate 5 with a thickness smaller than the depth of the groove 101.
A side pressure plate 4 is provided to restrict the top of the counter plate 5, preventing the counter plate 5 from coming off the top opening of the groove 101.
Referring to fig. 1 and 4, the driving assembly 2 includes a stepping motor 201, a motor gear 202, and a rack 203. The stepper motor 201 is fixed at the bottom of the wafer carrying platform 1 through bolts. The output end of the stepping motor 201 is in transmission connection with an output shaft. The end part of the output shaft vertically passes through the bearing piece platform 1, and is fixedly sleeved with a motor gear 202. One side of the motor gear 202 is engaged with a rack 203. The length direction of the rack 203 is consistent with the length direction of the groove 101. The side of the rack 203 with teeth faces away from the groove 101.
Referring to fig. 1 and 4, the linear guide 3 includes a sliding table 301 and a sliding block 302. A sliding table 301 is fixed on the carrying platform 1 through bolts. The guiding direction of the sliding table 301 is parallel to the length direction of the groove. And the sliding table 301 is connected with a sliding block 302 in a sliding way.
Referring to fig. 2, the moving assembly 7 includes a moving plate 701, a semi-cylindrical groove 702, a cylinder 703 and a connecting boss 704. An integrally formed boss 704 is fixed on the back side of the rack 203. The side portion of the movable plate 701 facing the boss 704 is fixed to the slider 302 by bolts. The movable plate 701 is provided with a semi-cylindrical groove 702 with a length arranged up and down towards the side wall of the boss 704. The boss 704 is welded with a cylinder 703 on the side facing the moving plate 701. The cylinder 703 is embedded in a semi-cylindrical groove 702. The bottom height of the movable plate 701 is lower than the bottom height of the counter plate 5.
A camera lens (not shown) is provided above the moving plate 701.
Referring to fig. 3 and 5, the other side of the movable plate 701 extends into the recess 101. And the edge of the movable plate 701 in the groove 101 is fixed with one half of the hinge of the spring hinge 6 through bolts. The bottom surface welding of the other half hinge of spring hinge 6 has buckle 8 that the tip has the voussoir. The inclined lower end of the wedge block at the end part of the buckle 8 faces the middle part of the spring hinge 6. The outer ends of the wedge blocks at the end parts of the buckles 8 are lapped on the stop blocks 9. The stop 9 spans the recess 101. And both end portions of the stopper 9 are fitted into the gap between the side pressure plate 4 and the carrier plate 1. The stop 9 is arranged above the counter plate 5.
The end of the counting plate 5 facing the moving plate 701 is provided with a clamping hole which is mutually clamped with the clamping buckle 8. The counting plate 5 is provided with a plurality of observation areas.
The working principle of the embodiment is that a counting plate 5 is inserted along an inlet of a groove 101, after the head end of the counting plate 5 is abutted to a moving plate 701, the moving plate 701 is pushed to move forward, then the moving plate 701 drives a spring hinge 6 at the top to move forward, a buckle 8 at the bottom of the other hinge of the spring hinge 6 is far away from a stop block 9, the inclined plane of a wedge block at the end of the buckle 8 moves in a relative dislocation manner with the contact surface of the stop block 9, after the inclined plane of the bottom of the wedge block at the end of the buckle 8 is completely separated from the top surface of the stop block 9, the buckle 8 rotates downwards under the elastic action of the spring hinge 6, then the buckle 8 clamps a clamping hole at the front end of the counting plate 5, at the moment, the external force is removed, a stepping motor 201 is started, a motor gear 202 is driven to rotate, a rack 203 moves along the length direction of the groove 101, a connecting boss 704 at the back side of the rack 203 is driven to move, the connecting boss 704 is driven to move through the clamping action of a cylinder 702 and a semi-cylinder 703, the moving plate 701 is driven to move forward, the moving plate 701 drives the spring hinge 6 and the buckle 8 to be mutually clamped with the clamping hole, the buckle 8 is driven to move forward, after the end of the wedge block 8 is clamped with the stop block 9, the counting plate 5 is driven to move forward, the stepping motor 201 is stable, and after the counting plate 5 moves stably and the observation plate 5 moves along the observation plane, and the observation plate is separated from the stop block 9 through the stop block 9, and the observation plate 9; by arranging the spring hinge 6 and the buckle 8 connected to the bottom of the spring hinge 6, when the stepping motor 201 drives the counting plate 5 to advance, the elastic action of the spring hinge 6 counteracts the unstable movement of the sliding table 301, the sliding block 302, the rack 203 and the moving plate 701 caused by poor manufacturing precision, reduces the factors influencing the focal length, the counting plate 5 moves in the groove 101, the observation definition can be improved only by improving the precision of the groove 101, the camera lens is not required to be adjusted for many times, the influence of the assembly factors of the camera lens is eliminated, and in operation, the counting plate 5 is only required to be pushed into the groove 101, and the stepping motor 201 is started, so that the operation is simple.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
The above-described embodiments merely represent embodiments of the invention, the scope of the invention is not limited to the above-described embodiments, and it is obvious to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.
Claims (10)
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| CN202110150000X | 2021-02-03 | ||
| CN202110150000 | 2021-02-03 |
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| CN112945836A CN112945836A (en) | 2021-06-11 |
| CN112945836B true CN112945836B (en) | 2025-01-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN214749655U (en) * | 2021-02-03 | 2021-11-16 | 上海萌薇生物医疗科技有限公司 | Snap-on cell counting plate automatic throughput device |
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| US9602777B2 (en) * | 2008-04-25 | 2017-03-21 | Roche Diagnostics Hematology, Inc. | Systems and methods for analyzing body fluids |
| JP5672342B2 (en) * | 2013-07-09 | 2015-02-18 | 東洋製罐グループホールディングス株式会社 | Counting device |
| CN104111344B (en) * | 2014-05-23 | 2016-04-13 | 苏州日和科技有限公司 | The full-automatic sample introduction mechanism of a kind of flow cytometer |
| CN208653996U (en) * | 2018-07-26 | 2019-03-26 | 黎华 | Flow cytometer is used in a kind of detection of systemic loupus erythematosus |
| CN210119428U (en) * | 2019-05-21 | 2020-02-28 | 江西中洪博元生物技术有限公司 | Cell counting instrument with strong stability |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN214749655U (en) * | 2021-02-03 | 2021-11-16 | 上海萌薇生物医疗科技有限公司 | Snap-on cell counting plate automatic throughput device |
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