CN112940909A - Sample precision control instrument and use method thereof - Google Patents

Abstract

The invention belongs to the field of egg cell manipulation instruments, and discloses a sample precision control instrument and a use method thereof. Wherein, the sample precision control instrument includes a host room, a hand piece and a connecting tube, the host room is installed with a display and a driving mechanism, the driving mechanism is provided with a connection port, the hand piece includes a gripping part, and the gripping part A through connection channel is provided on the upper part, one end of the connection pipe is communicated with the end of the connection channel, and the other end of the connection pipe is communicated with the connection port. The drive mechanism is separated from the hand piece, which improves the control precision during the egg stripping operation.

Description

Sample precision control instrument and use method thereof

Technical Field

The invention belongs to the field of egg cell and embryo operation instruments, and particularly relates to a sample precision control instrument and a use method thereof.

Background

The "tube baby" is a baby born by artificially fertilizing ova and sperms in vitro, developing an early embryo, and then transplanting the embryo to a mother uterus. In the whole in vitro fertilization and cultivation process, egg stripping and sample transfer are important contents in the daily work of the assisted reproductive laboratory. The egg stripping is to strip granular cells around the ovum and expose the ovum, and is mainly used for evaluating the maturity of the oocyte, evaluating the fertilization condition of the ovum, facilitating the freezing of the ovum and the micromanipulation, and the operation details in the egg stripping process directly influence the effect of the subsequent operation links; the sample transfer relates to the process of transferring various sperms, ova and fertilized ova from a culture medium to other culture media, and has higher requirements on the stability and the accuracy of the operation.

Among the present ovum instrument of shelling, like chinese patent, application number 201310034724.3 discloses a novel test tube baby shells ovum manipulator, uses the hand operation, forms the pressure differential through the extrusion softgel, and then absorbs liquid, but this mode all relies on artifical pressing method operation at every turn, can't control the dynamics of absorbing to and press the in-process hand hard and lead to the shake easily, so the condition that can't absorb the completion appears easily together during the operation, lead to control accuracy greatly reduced, influence operating efficiency.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide a sample precision control instrument and a using method thereof, which can improve the control precision during egg stripping operation and sample transfer.

The technical scheme is as follows:

sample essence accuse appearance includes host computer room, handheld piece and connecting pipe, display and actuating mechanism are installed to the host computer room, actuating mechanism is provided with the connector, the handheld piece is including holding the portion of holding, be provided with the interface channel who link up on holding the portion, connecting pipe one end with interface channel's end is linked together, the connecting pipe other end with the connector is linked together.

In one embodiment, the driving mechanism comprises a power piece and a plunger piece, the bottom of the plunger piece is provided with the connecting port, and the power piece drives the plunger piece to move.

In one embodiment, the driving mechanism further includes a transmission member, the power member is a linear motion motor, the plunger member includes a plunger rod and a plunger sleeve, the plunger rod is matched with the plunger sleeve, the bottom of the plunger sleeve is provided with the connection port, one end of the transmission member is connected with the output end of the linear motion motor, and the other end of the transmission member is connected with the plunger rod.

In one embodiment, the transmission member comprises a guide block and a transmission block, the guide block has a first end and a second end, the first end and the second end form a right angle, the first end is in sliding fit with a shell of the linear motion motor, the second end is connected with an output end of the linear motion motor, the guide block is fixedly connected with the second end of the guide block, and the guide block is simultaneously connected with one end of the plunger rod.

In one embodiment, the main machine chamber is further provided with an adapter, the adapter is connected with the lower end of the plunger sleeve, the connecting port is positioned in the adapter, and the connecting pipe is communicated with the adapter.

In one embodiment, the connecting channel of the grip is further connected with a fallopian tube.

In one embodiment, a bearing part is arranged outside the main unit chamber, two side parts of the bearing part are protruded, and the hand-held part can be placed between the protrusions of the bearing part.

In one embodiment, the drive mechanism, the connecting tube and the handpiece are filled with a liquid.

In one embodiment, the main machine room is further provided with a foot pedal interface.

The use method of the sample precision control instrument comprises the following steps:

the upper oviduct peeling pipe is arranged on the gripping part;

starting a power supply of the host computer room, displaying by the display and starting a program;

the holding hand piece is held and moved to a pre-use position, the driving mechanism is started to generate pressure difference, the connecting pipe is communicated with the connecting port, and the pressure difference is transmitted to the connecting channel of the holding part under the conduction of the connecting pipe and is further transmitted to the oviduct peeling part;

the oviduct can suck and spray liquid or liquid containing egg cells due to the pressure difference.

The technical scheme provided by the invention has the following advantages and effects:

1. after the assembly is completed, the host computer room is started, the display of the host computer room can display the started program, and then the hand piece is held, and the hand piece is provided with the oviduct. The host computer room starts, and actuating mechanism starts to produce the pressure differential, then conducts on the connecting channel of gripping part under the effect of connecting pipe, so peel the ovum tube and can absorb and spout liquid and the liquid that contains the ovum cell under the effect of pressure differential. The operation is to grab the hand-held piece in the process, and the hand-held piece is not provided with a driving mechanism, so that the structure is simpler, the hand-held piece is lighter, and the hand-held piece is not easy to fatigue after being grabbed for a long time. In addition, because the driving mechanism is arranged on the main machine chamber, when liquid absorption and liquid discharge are needed, power is provided through the main machine chamber, so that vibration is not transmitted to the hand piece, and the control precision of the hand piece is greatly improved during operation.

2. The pressure difference generated by the driving mechanism needs to be transmitted through the connecting pipe, if gaseous medium is directly adopted for transmission, the transmission hysteresis can be caused due to the obvious compression effect, the error amplification can be caused to each small-amount egg stripping operation for sucking, and the liquid suction precision is influenced. The connecting pipe and the driving mechanism are filled with liquid in the plunger sleeve and the connecting channel of the hand piece, the liquid has poor compression performance, so the transmission efficiency is high, and the differential pressure transmitted by the driving mechanism can be quickly transmitted to the oviduct dissecting pipe with low delay and low error.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles and effects of the invention.

Unless otherwise specified or defined, the same reference numerals in different figures refer to the same or similar features, and different reference numerals may be used for the same or similar features.

FIG. 1 is a schematic perspective view of an embodiment of the present invention;

FIG. 2 is a schematic structural view of the embodiment of the present invention with the protective plate removed;

FIG. 3 is a schematic back view of an embodiment of the present invention;

FIG. 4 is a schematic structural view of a handpiece in accordance with an embodiment of the invention;

FIG. 5 is an exploded view of an embodiment of the present invention;

FIG. 6 is a cross-sectional structural schematic view of the drive mechanism of an embodiment of the present invention prior to actuation;

FIG. 7 is a schematic sectional view of the drive mechanism of the embodiment of the present invention in operation;

FIG. 8 is a schematic perspective view of the actuator engaging the adapter according to an embodiment of the present invention;

fig. 9 is a schematic cross-sectional view of the driving mechanism and the adaptor according to the embodiment of the present invention.

Description of reference numerals:

10. a main machine room; 11. a display; 12. a drive mechanism; 121. a power member; 1211. a linear motion motor; 122. a plunger member; 1221. a plunger rod; 1222. a plunger sleeve; 123. a transmission member; 1231. a guide block; 1232. a transmission block; 13. an adapter; 131. a transfer port; 14. a holding member; 15. a pedal interface; 16. a position detector; 17. an operation button; 18. a support member; 20. a handpiece; 21. a grip portion; 30. and (4) connecting the pipes.

Detailed Description

In order to facilitate an understanding of the invention, specific embodiments thereof will be described in more detail below with reference to the accompanying drawings.

Unless specifically stated or otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the case of combining the technical solutions of the present invention in a realistic scenario, all technical and scientific terms used herein may also have meanings corresponding to the purpose of achieving the technical solutions of the present invention.

As used herein, unless otherwise specified or defined, "first" and "second" … are used merely for name differentiation and do not denote any particular quantity or order.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items, unless specified or otherwise defined.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present; when an element is referred to as being "mounted on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "on" another element, it can be directly on the other element or intervening elements may also be present.

In this embodiment, the present invention can be used for manipulation of egg cells and embryos, and in this embodiment, manipulation of egg cells is taken as an example.

As shown in fig. 1 to 6, the sample precision control instrument includes a main machine room 10, a hand-held piece 20 and a connection pipe 30, the main machine room 10 is provided with a display 11 and a driving mechanism 12, the driving mechanism 12 is provided with a connection port, the hand-held piece 20 includes a holding part 21, a through connection channel is provided on the holding part 21, one end of the connection pipe 30 is communicated with the tail end of the connection channel, and the other end of the connection pipe 30 is communicated with the connection port. The connecting channel of the gripping part 21 is also connected with a fallopian tube.

After assembly is completed, the main housing 10 is started, the display 11 of the main housing 10 displays the started program, and then the hand piece 20 is held, wherein the hand piece 20 is provided with the oviduct. The main machine chamber 10 is started, the driving mechanism 12 is started to generate a pressure difference, and then the pressure difference is transmitted to the connecting channel of the gripping part 21 under the action of the connecting pipe 30, so that the oviduct can suck and spray liquid and liquid containing egg cells under the action of the pressure difference. The operation is to grasp the hand piece 20 during this process, and the absence of the actuating mechanism 12 on the hand piece 20 is relatively simple in construction, thus also making the hand piece 20 relatively light and less tiring to grasp for a long time. In addition, since the driving mechanism 12 is provided in the main housing 10, when liquid suction and discharge are required, power is supplied through the main housing 10, and thus a vibration feeling is not transmitted to the hand piece 20, so that the accuracy of control of the hand piece 20 is greatly improved during operation.

The sample transfer tube used in the egg peeling mode needs to be used when a sample is transferred, and the space of the sample transfer tube is larger than that of the egg peeling tube. The sample transfer tube has large space and can be suitable for embryo transfer.

As shown in fig. 5 to 7, the driving mechanism 12 includes a power element 121 and a plunger element 122, the bottom of the plunger element 122 is provided with the connecting port, and the power element 121 drives the plunger element 122 to move. Movement of plunger member 122 creates a pressure differential that is transmitted through connecting tube 30 to hand piece 20.

Specifically, the driving mechanism 12 further includes a transmission member 123, the power member 121 is a linear motion motor 1211, the plunger member 122 includes a plunger rod 1221 and a plunger sleeve 1222, the plunger rod 1221 is engaged with the plunger sleeve 1222, the bottom of the plunger sleeve 1222 is provided with the connection port, one end of the transmission member 123 is connected to the output end of the linear motion motor 1211, and the other end of the transmission member is connected to the plunger rod 1221. The linear motion motor 1211 moves linearly, and then drives the plunger rod 1221 to move up and down under the action of the transmission member 123, the plunger rod 1221 is in sliding fit with the plunger sleeve 1222, and a pressure difference generated by the up and down movement of the plunger rod 1221 is transmitted to the connecting pipe 30 through the connecting port.

As shown in fig. 1 and 2, the plunger member 122 is provided with a spare part, which is installed on the main machine room 10 and closed by a protection plate, and the protection plate is removed from the plunger member 122 when the plunger member 122 needs to be replaced.

As shown in fig. 6 and 7, the transmission member 123 includes a guide block 1231 and a transmission block 1232, the guide block 1231 has a first end and a second end, the first end and the second end are at right angles, the first end is slidably engaged with the housing of the linear motion motor 1211, the second end is connected with the output end of the linear motion motor 1211, the guide block 1231 is fixedly connected with the second end of the guide block 1231, and the guide block 1231 is connected with one end of the plunger rod 1221. Because the output end of the linear motion motor 1211 cannot provide transverse stress and the output end is protected from being damaged by the transverse force, the guide block 1231 is arranged, the guide block 1231 is in sliding fit with the outer shell of the linear motion motor 1211, the second end is used for stress, the plunger rod 1221 is connected through the transmission block 1232, the linear motion motor 1211 is used for driving the plunger rod 1221 to move, and the problem of sliding precision can be solved. In the case of egg peeling requiring high precision, the precision can be improved while protecting the linear motion motor 1211 and the plunger member 122 from the lateral force.

As shown in fig. 8 and 9, an adaptor 13 is further installed on the main housing 10, the adaptor 13 is connected to the lower end of the plunger sleeve 1222, the connection port is located in the adaptor 13, and the connection pipe 30 is communicated with the adaptor 13. The adapter 13 forms a medium for connecting the plunger sleeve 1222 to the connecting tube 30, and the adapter 13 serves to fix the plunger sleeve 1222. The adaptor 13 is provided with two adaptor ports 131, wherein one adaptor port 131 is used for communicating the connecting pipe 30, the other adaptor port 131 is blocked, and the first adaptor port 131 is communicated with the second adaptor port 131.

The drive mechanism 12, the connecting tube 30 and the hand piece 20 are filled with liquid. The pressure difference generated by the driving mechanism 12 needs to be transmitted through the connecting pipe 30, if gaseous medium transmission is directly adopted, the transmission hysteresis can be caused because of obvious compression action, and the error amplification can be caused for each small-amount egg stripping operation of suction, so that the liquid suction precision is influenced. Here, since the connection tube 30 and the driving mechanism 12, specifically, the plunger sleeve 1222 and the connection passage of the hand piece 20 are filled with liquid, the liquid has poor compressibility and thus has high conduction efficiency, and the differential pressure conducted by the driving mechanism 12 can be quickly conducted to the tubal denudation with low delay and low error. In this example, liquid paraffin oil was used.

When liquid is injected, the liquid is injected through the second adapter 131, and the liquid is plugged after the liquid is filled, so that the liquid needing to be supplemented and the liquid needing to be discharged can be completed through the second adapter 131.

As shown in fig. 1 and 2, a receiving member 14 is disposed outside the main housing 10, two side portions of the receiving member 14 are protruded, and the hand piece 20 can be placed between the protrusions of the receiving member 14. The hand piece 20 may be placed on the support member 14 during the operating gap without being placed on a table, contaminating the hand piece 20, or even inadvertently damaging the hand piece 20.

As shown in fig. 3, the main cabinet 10 is further provided with a foot interface 15. The control of the drive mechanism 12 by hand while holding the hand piece 20 in hand is avoided by controlling the actuation of the drive mechanism 12 by foot pedal during the egg stripping and egg removal operations.

A power inlet is also provided at the back of the main cabinet 10, and a foot pedal interface 15 is also provided at the back of the main cabinet 10.

As shown in fig. 4, the bottom of the main cabinet 10 is provided with a support, which is foldable. When the main cabinet 10 is folded, the main cabinet 10 is laid flat, and when the main cabinet 10 is unfolded, the main cabinet 10 has an inclined angle, so that the display 11 can be conveniently viewed.

As shown in fig. 1 and 2, the main machine room 10 is provided with a control button 17, which is used to select program settings, but it is also possible to configure the display 11 as a touch screen for direct touch control at increased cost.

As shown in fig. 8, a position detector 16 is provided inside the main cabinet 10, and a barrier is provided on the guide block 1231, and the position detector 16 detects the position of the barrier and controls the movement limit of the driving linear motion motor 1211.

The use method of the sample precision control instrument comprises the following steps: the grasping part 21 is provided with an upper oviduct; starting the power supply of the main machine room 10, displaying by the display 11 and starting a program; holding the hand piece 20, moving to the pre-use position, starting the driving mechanism 12 to generate pressure difference, communicating the connecting pipe 30 with the connecting port, and conducting the pressure difference to the connecting channel of the holding part 21 under the conduction of the connecting pipe 30 to continue to conduct to the oviduct; the oviduct can suck and spray liquid or liquid containing egg cells due to the pressure difference.

The program of the sample precision control instrument is provided with a plurality of modes, such as an egg stripping mode and a sample transfer mode.

Egg stripping mode, the operation of removing peripheral granulosa cells for the cumulus oophorus complex. Under the ovum peeling mode, the liquid retention amount and the liquid absorption and spitting amount during operation are set, the liquid retention amount is the liquid amount sucked in advance, the balance liquid amount can be understood, the part of the liquid amount can be used for balancing the internal and external pressure of a new ovum peeling pipe after the new ovum peeling pipe is immersed in the liquid, the set amount is sucked, after the whole ovum peeling operation is finished, the part of the liquid retention amount can be reserved in the ovum peeling pipe and cannot be knocked out, and a sample can be effectively prevented from being adhered or being left in the ovum peeling pipe. The liquid suction amount and the liquid discharge amount are equivalent to each other.

And a sample transfer mode, which is a sample transfer operation. In the sample transfer mode, two parameters are commonly used, one is a lower liquid spitting limit, the other is an upper liquid absorption limit, the upper liquid absorption limit is an upper liquid absorption amount limit, and the upper limit is set to prevent liquid from being sucked without limit, so that liquid pollution and even sample loss are caused. The lower limit of the liquid discharge is a lower limit of the liquid reserved after the liquid discharge is limited, and a large amount of bubbles are blown out to prevent an unlimited liquid discharge operation. The lower limit of liquid discharge is set to 0 to 99.9. mu.L, and the upper limit of liquid suction is set to 0.1 to 99.9. mu.L, depending on the size of the sample transfer tube.

The liquid absorbing and spitting speed can be set, the liquid absorbing and spitting speed is graded, the low-grade speed, the medium-grade speed and the high-grade speed are common, and more speeds can be set in different use scenes. After the liquid absorbing and spitting speed is set, the speed is set in the egg peeling mode and the sample transfer mode, the speed is output by controlling the speed through a pedal externally connected with the host computer room 10, the right pedal button is set for selecting the egg peeling mode, and the left pedal button is defaulted to be the low-speed egg peeling mode; in the sample transfer mode, according to the set lower liquid discharging limit, upper liquid suction limit and speed, the right foot button is used for sucking operation, and the left foot button is used for discharging operation.

The specific setting is of course set according to the use requirements, and only one case is enumerated here.

When the drawing description is quoted, the new characteristics are explained; in order to avoid that repeated reference to the drawings results in an insufficiently concise description, the drawings are not referred to one by one in the case of clear description of the already described features.

The above embodiments are provided to illustrate, reproduce and deduce the technical solutions of the present invention, and to fully describe the technical solutions, the objects and the effects of the present invention, so as to make the public more thoroughly and comprehensively understand the disclosure of the present invention, and not to limit the protection scope of the present invention.

The above examples are not intended to be exhaustive of the invention and there may be many other embodiments not listed. Any alterations and modifications without departing from the spirit of the invention are within the scope of the invention.

Claims (10)

1. Sample essence accuse appearance, its characterized in that, including host computer room, handheld piece and connecting pipe, display, actuating mechanism and are installed to the host computer room, actuating mechanism is provided with the connector, handheld piece is including holding the portion, be provided with the connected channel who link up on holding the portion, connecting pipe one end with connected channel's end is linked together, the connecting pipe other end with the connector is linked together.

2. The sample fine control instrument according to claim 1, wherein the driving mechanism comprises a power piece and a plunger piece, the bottom of the plunger piece is provided with the connecting port, and the power piece drives the plunger piece to move.

3. The sample precision control instrument according to claim 2, wherein the driving mechanism further comprises a transmission member, the power member is a linear motion motor, the plunger member comprises a plunger rod and a plunger sleeve, the plunger rod is matched with the plunger sleeve, the bottom of the plunger sleeve is provided with the connecting port, one end of the transmission member is connected with the output end of the linear motion motor, and the other end of the transmission member is connected with the plunger rod.

4. The sample fine control instrument according to claim 3, wherein the transmission member comprises a guide block and a transmission block, the guide block has a first end and a second end, the first end and the second end are at right angles, the first end is in sliding fit with the housing of the linear motion motor, the second end is connected with the output end of the linear motion motor, the guide block is fixedly connected with the second end of the guide block, and the guide block is simultaneously connected with one end of the plunger rod.

5. The sample precision control instrument according to claim 3, wherein an adaptor is further installed on the main machine chamber, the adaptor is connected with the lower end of the plunger sleeve, the connecting port is located in the adaptor, and the connecting pipe is communicated with the adaptor.

6. The sample precision control instrument according to any one of claims 1 to 5, wherein a fallopian tube is further connected to the connecting channel of the grip portion.

7. The sample precision control instrument according to any one of claims 1 to 5, wherein a holding member is arranged outside the main machine chamber, two side parts of the holding member are protruded, and the hand-held member can be placed between the protrusions of the holding member.

8. The sample precision control instrument according to any one of claims 1 to 5, wherein the driving mechanism, the connecting tube and the hand piece are filled with liquid.

9. The precision sample control instrument according to any one of claims 1 to 5, wherein the main machine chamber is further provided with a foot interface.

10. The use method of the sample precision control instrument is characterized by comprising the following steps:

the upper oviduct peeling pipe is arranged on the gripping part;

starting a power supply of the host computer room, displaying by the display and starting a program;

the holding hand piece is held and moved to a pre-use position, the driving mechanism is started to generate pressure difference, the connecting pipe is communicated with the connecting port, and the pressure difference is transmitted to the connecting channel of the holding part under the conduction of the connecting pipe and is further transmitted to the oviduct peeling part;

the oviduct can suck and spray liquid or liquid containing egg cells due to the pressure difference.

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