CN112932988B

CN112932988B - Anti-aging skin care product containing polydeoxyribonucleic acid

Info

Publication number: CN112932988B
Application number: CN202110342119.7A
Authority: CN
Inventors: 包磊
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-03-30
Filing date: 2021-03-30
Publication date: 2022-03-15
Anticipated expiration: 2041-03-30
Also published as: CN112932988A

Abstract

The invention relates to the technical field of cosmetics, and provides an anti-aging composition containing polydeoxyribonucleic acid, which comprises honey and polydeoxyribonucleic acid; the invention also discloses a skin care product, which comprises the anti-aging composition containing polydeoxyribonucleic acid and an auxiliary agent acceptable in cosmetics. The anti-aging composition and the skin care product comprising the anti-aging composition disclosed by the invention enhance the effects of skin cell regeneration and anti-aging through the synergistic effect of honey and polydeoxyribonucleic acid.

Description

Anti-aging skin care product containing polydeoxyribonucleic acid

Technical Field

The invention relates to the technical field of cosmetics, in particular to an anti-aging skin care product containing polydeoxyribonucleic acid.

Background

The skin is an organ which is wrapped on the surface of the body, is directly contacted with the external environment, has the functions of protecting, excreting, regulating body temperature, sensing external stimulation and the like, and is the largest organ of the human body. However, with the aging and the influence of the external environment, the skin will gradually age, so that the protection ability and the regulation ability of the skin to the body are reduced, so that the skin can not adapt to the change of the internal and external environment, and the change of the overall appearance conditions such as color, shape, texture and the like, such as wrinkles, elasticity reduction, skin relaxation, roughness, pigmentation spot formation and the like, brings troubles to many beauty lovers, and therefore, the international beauty industry also continuously puts forward anti-aging beauty products.

Because chemical cosmetic products can cause different degrees of damage to the skin, cosmetic manufacturers gradually move from chemical synthesis to pure natural ingredients. Although many of the prior art formulations have skin cell regenerating and anti-aging effects, the results are not ideal.

Therefore, it is highly desirable to provide an anti-aging composition capable of enhancing cell regeneration and having more excellent anti-aging effect, and a skin care product comprising the same.

Disclosure of Invention

The invention aims to provide an anti-aging composition capable of enhancing cell regeneration capacity and having more excellent anti-aging effect and a skin care product containing the anti-aging composition.

In view of the above, the first objective of the present invention is to provide an anti-aging composition containing polydeoxyribonucleic acid.

The second purpose of the invention is to provide a skin care product of the anti-aging composition containing polydeoxyribonucleic acid.

In order to achieve the purpose, the invention adopts the following technical scheme:

an anti-aging composition containing polydeoxyribonucleic acid, which comprises the following components: honey and polydeoxyribonucleic acid.

Preferably, the honey is white honey.

The beneficial effects of the above technical scheme are that: honey, here white honey from the Wendel garden, contains vitamins, minerals and trace elements that make it effective in healing wounds. Meanwhile, the honey contains saccharides containing hydroxyl groups such as fructose, so that the honey has a good moisturizing effect. If the skin is not moisturized, the ability of cells to regenerate is reduced, the elasticity is reduced, wrinkles occur, and skin aging is promoted.

In the human body, highly reactive compounds derived from oxygen are called free radicals and Reactive Oxygen Species (ROS), which are produced during metabolism. These free active compounds are capable of reacting with many lipids and proteins present on the cell membrane, and also with DNA and enzymes present in the cell. This destructive reaction can have effects such as aging. Antioxidants can intercept free radicals and thus prevent them from damaging the reaction. Protection mechanisms involved in antioxidants include enzymes (peroxidases) and non-enzymes (tocopherols, phenolic compounds, flavonoids, catechins, ascorbic acid and carotenoids). When the honey reaches a certain concentration, the honey can generate the effect of inhibiting oxidation of lipoprotein in vitro, namely has antioxidant capacity. At the same time, honey can stimulate non-specific reaction. The process of macrophage activation by stimulation with a specific substance is called non-specific immune response (NSIR), and the activation of macrophages can produce cytokines. Cytokines (CK) are low molecular weight soluble proteins that are produced by various cells induced by immunogens, mitogens or other stimulants and have a variety of functions such as modulating innate and adaptive immunity, hematopoiesis, cell growth and damaged tissue repair. Since activation of the immune system decreases with age, cell regeneration decreases. But the anti-aging effect can be achieved by using the anti-aging agent containing the substances capable of stimulating non-specific reactions. The honey can stimulate the immune system, so the honey has the function of cell regeneration and further has the effect of anti-aging.

Preferably, the polydeoxyribonucleic acid is derived from a salmon sperm DNA controlled purification and sterilization process.

The beneficial effects of the above technical scheme are that: the epidermis, which determines the properties of the skin, is directly and frequently damaged from the outside, and therefore recovery of wounds is very important. The basal layer cells, which are located innermost in the epidermis, are divided into the stratum corneum while undergoing proliferation and division to maintain an overall bilayer structure, thereby protecting the skin surface from the external harmful environment. However, the outer skin cells, which are the outermost part of the skin, age and are removed, and cell regeneration is a process of replacing their aged cells, which is repeated. When external skin damage occurs, cell regeneration occurs rapidly, and recovery should be promoted. With age, the proliferation of fibroblasts that repair skin lesions decreases, and in turn the synthesis of connective tissue collagen decreases, resulting in a decrease in the regenerative capacity of the cells.

Polydeoxyribonucleic acid (PDRN) is derived from salmon sperm DNA through controlled purification and sterilization processes (with 95% similarity to human DNA), and is a new favorite in the beauty industry due to the function of promoting cell regeneration. In vitro and in vivo experiments show that the action mechanism of PDRN is the participation of adenosine A2A receptor, and PDRN efficiently and specifically activates A2A adenosine receptor on the cell surface, thereby promoting the release of cytokines, generating anti-inflammatory action and repairing damaged cells. The activation of the receptor can proliferate osteoblasts, fibroblasts and lipo precursor cells, and activate aged damaged cells to make them younger. Besides, VEGF and angiogenin are released along with the activation of receptors, so that the blood circulation is improved, and the cell regeneration is accelerated. Therefore, the PDRN has good functions of recovering the skin elasticity and repairing scars.

Polydeoxyribonucleic acid and white honey are commercially available products.

Preferably, the mass ratio of the honey to the polydeoxyribonucleic acid is (10-100): 1.

preferably, the mass ratio of the honey to the polydeoxyribonucleic acid is (25-75): 1.

further preferably, the mass ratio of the honey to the polydeoxyribonucleic acid is (40-60): 1.

preferably, the mass ratio of honey to polydeoxyribonucleic acid may be 10:1, 15:1, 20:1, 25:1, 30:1, 35:1, 40:1, 45:1, 50:1, 55:1, 60:1, 65:1, 70:1, 75:1, 80:1, 85:1, 90:1, 95:1 or 100: 1.

A skin care product containing polydeoxyribonucleic acid as antiaging composition is provided.

Preferably, the anti-aging skin care product further comprises an auxiliary agent acceptable in cosmetics.

Preferably, the auxiliary agent comprises a humectant, a conditioning oil, a thickening agent, an acid-base regulator, a cosolvent and/or an emulsifier.

Further preferably, the humectant is one or more selected from hyaluronic acid, betaine, trehalose and sodium polyglutamate.

Further preferably, the conditioning oil is selected from one or more of squalane, avocado oil, meadowfoam seed oil, acetic acid capric acid triglyceride, dioctyl carbonate, cetostearyl alcohol and behenyl alcohol.

Further preferably, the thickening agent is one or more selected from polyacrylic acids, celluloses, xanthan gum and sclerotium rolfsii gum.

Further preferably, the acid-base regulator is one or more selected from triethanolamine, potassium hydroxide, sodium hydroxide, arginine, citric acid, glycolic acid, lactic acid and hydrochloric acid.

Further preferably, the cosolvent is one or more selected from polysorbate-80, hydrogenated castor oil, poloxamer, propylene glycol, butylene glycol, ethanol, and the like.

Further preferably, the emulsifier is selected from one or more of lecithin, glycerol stearate, PEG-100 stearate, hydrogenated lecithin, polyglycerol-3 polyricinoleate, polyglycerol-3 ricinoleate, inulin lauryl carbamate, acryloyl dimethyl taurate-based polymer, bis-PEG/PEG-20/5 PEG/PEG-20/5 polydimethylsiloxane and methoxy PEG/PEG-25/4 polydimethylsiloxane.

Preferably, the anti-aging composition accounts for more than or equal to 0.5% of the skin care product by mass, and more preferably 0.5% -2%.

Specifically, the anti-aging composition may be present in the skin care product at 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, or 2.0% by mass.

Preferably, the mass fraction of polydeoxyribonucleic acid in the skin care product is not less than 0.004%, and more preferably 0.004-0.2%.

Preferably, the mass fraction of polydeoxyribonucleic acid in the skin care product may be 0.004%, 0.006%, 0.007%, 0.008%, 0.009%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, 0.21%, 0.22%.

Preferably, the skin care product comprises cream, essence, stock solution, essence water, mask or eye cream.

The beneficial effects of the above technical scheme are that:

1. the synergistic effect of honey and PDRN enhances the effects of skin cell regeneration and anti-aging. The PDRN has the functions of recovering skin elasticity and repairing scars, and the vitamins, minerals and trace elements contained in the honey can effectively nourish the skin and facilitate the repair of barrier function. Meanwhile, the honey contains saccharides containing hydroxyl groups such as fructose, so that the honey has a good moisturizing effect and the cell regeneration capacity is enhanced. The honey can also stimulate the immune system, stimulate the production of cell growth factors, regenerate cells and further produce the anti-aging effect.

2. The combination of honey and PDRN is natural in source, safe and effective.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The reagents and raw materials used in the following examples are all commercially available products, and PDRN is produced by ruddy biotechnology limited.

Examples 1 to 10

100g of the composition is prepared, the components of honey and PDRN are mixed according to the following weight, and the specific formula is shown in table 1.

TABLE 1

Example 11

The compositions prepared in examples 1 to 10 of the present invention can be used in cosmetics as an active ingredient of cosmetics. The cosmetic may be in the form of cream, essence, stock solution, essence solution, facial mask, eye cream, or skin lotion, and comprises the matrix necessary for preparing various dosage forms, in addition to the composition containing Mel and PDRN.

The essence base is prepared, and the specific formula is shown in table 2.

TABLE 2 essence base formula

Name of raw materials	Ratio (%)
		Lecithin	0.5
Purified water	96.679
		Phytic acid	0.001
Hexanediol	0.20
		P-hydroxyacetophenone	0.50
Glycerol	2.00
		Sclerotium rolfsii gum	0.10
Aminomethyl propanol	0.02

The preparation method comprises the following steps:

adding purified water with the amount of 80% of the formula amount into a preparation tank, heating to 60-80 ℃, then sequentially adding the components in the formula table, stirring to dissolve completely, adding the purified water to 100% of the specified amount, and standing at room temperature for later use.

Examples 12 to 18

100g of essence containing the composition is prepared, a proper amount of the substrate in the embodiment 11 is taken, a certain amount of the composition in the embodiment 5 is added, and the mixture is uniformly stirred, wherein the specific formula is shown in a table 3.

TABLE 3

	Substrate (g)	The composition is added in an amount (g)	PDRN content (g)
				Example 12	99.7	0.3	0.00732
Example 13	99.6	0.4	0.00976
				Example 14	99.5	0.5	0.0122
Example 15	99.0	1.0	0.0224
				Example 16	98.5	1.5	0.0366
Example 17	98	2.0	0.0488
				Example 18	97.5	2.5	0.061

Examples 19 to 24

100g of essence containing the composition is prepared, a proper amount of the substrate in the embodiment 11 is taken, a certain amount of the composition in the embodiment 1 is added, and the mixture is uniformly stirred, wherein the specific formula is shown in a table 4.

TABLE 4

	Substrate (g)	The composition is added in an amount (g)	PDRN content (g)
				Example 19	99.6	0.4	0.03636
Example 20	99.5	0.5	0.04545
				Example 21	99	1.0	0.0909
Example 22	98.5	1.5	0.13635
				Example 23	98	2.0	0.1818
Example 24	97.5	2.5	0.22725

Examples 25 to 30

100g of essence containing the composition is prepared, a proper amount of the substrate in the embodiment 11 is taken, a certain amount of the composition in the embodiment 10 is added, and the mixture is uniformly stirred, wherein the specific formula is shown in a table 5.

TABLE 5

	Substrate (g)	The composition is added in an amount (g)	PDRN content (g)
				Example 25	99.6	0.4	0.00396
Example 26	99.5	0.5	0.00495
				Example 27	99	1.0	0.0099
Example 28	98.5	1.5	0.01485
				Example 29	98	2.0	0.0198
Example 30	97.5	2.5	0.02475

Comparative example 1

Preparing 100g of essence containing the composition, taking 90g of the substrate in the example 11, adding 10g of white honey, and uniformly stirring.

Comparative example 2

100g of essence containing the composition was prepared by adding 1g of PDRN to 99g of the base material of example 11 and stirring the mixture uniformly.

Comparative example 3

100g of essence containing the composition was prepared by adding 0.2g of PDRN to 99.9g of the base material of example 11 and stirring the mixture uniformly.

And (3) experimental verification:

1. effect on fibroblast proliferation

Thawing frozen human fibroblasts (HSF), culturing in a 60 mm-diameter culture dish for 48h, subculturing to a 100 mm-diameter culture dish for 24h until 80% fusion, digesting the HSF cells which grow vigorously with pancreatin, adjusting the cell density to 5 × 104/mL, and inoculating to a 96-well cell culture plate.

Setting sample group, control group (cell + culture medium), blank control group (no cell + culture medium), adding 100 μ L cell suspension into each well of sample group and control group, placing in carbon dioxide incubator at 37 deg.C and 5% CO₂After the conventional culture for 24 hours, the culture solution was discarded. Then, the sample components were replaced with 100. mu.L of solutions containing 2.0% of the compositions of examples 1 to 10 (prepared by weighing 2.0g of the compositions of examples 1 to 10, adding 98g of the essence base of example 11, and stirring them uniformly) of examples 12 to 16, 18 to 22, 24 to 28, and 30 and comparative examples 1 to 3, respectively. The control group and the blank control group were added with the same amount of serum-free culture solution. Each group is provided with 6 parallel holes, and is placed in a carbon dioxide incubator at 37 ℃ and 5% CO₂Culturing for 48 hr, adding 10 μ L of WST-1 solution into each well, placing in carbon dioxide incubator at 37 deg.C and 5% CO₂Culturing in dark for 2h, measuring absorbance at 450nm with microplate reader, and calculating relative proliferation rate (RGR) according to the following formula.

RGR＝A/A₀×100％

In the formula: RGR-relative proliferation rate,%;

a is absorbance of sample group, and blank control group is deducted;

A₀-control absorbance, blank control subtracted.

TABLE 6 Effect on fibroblast proliferation

Human skin fibroblasts are the main structural components constituting the dermis of the skin, can synthesize and secrete extracellular matrices such as collagen fibers, elastic fibers, reticular fibers, hyaluronic acid and the like, and have important effects on maintaining the strength and elasticity of the skin, repairing injuries and the like. The activity of fibroblasts is directly related to the amount of collagen, elastic fibers and hyaluronic acid produced by the fibroblasts, so that the proliferation capacity of the fibroblasts can reflect the degree of influence of certain substances on the skin condition.

The above experimental results show that the compositions of examples 1-10 have better fibroblast proliferation promoting ability than comparative examples 2 and 3(PDRN), and are higher than the technical effects obtained by comparative examples 2 and 3, wherein the compositions of examples 5-7 have the best effect. Examples 4 to 1 each had an increasing PDRN content, and the proliferation ability of fibroblasts was not significantly different and tended to decrease, but the mass fraction ratio of honey to PDRN in the composition was set to 10:1 at the lowest and 100:1 at the highest, considering the cost and effect of PDRN, because of its high cost.

Examples 12 to 30, the effect was gradually increased as the amount of the composition was gradually increased, but the effect was gradually decreased after increasing to a certain extent because the surplus of nutrients did not contribute to cell growth, and the composition was added to the cosmetic in an amount of 0.5 to 2% based on the total amount of the cosmetic in consideration of cost and effect, wherein the PDRN was preferably contained in an amount of 0.004 to 0.2% based on the total amount of the cosmetic.

2. And (3) clinical verification:

2.1 selecting volunteers

45 volunteers are selected, the age is 25-45 years old, and the number of the volunteers is not limited by men and women. The complaints of each volunteer reflected the problem of skin dullness and laxity, 8 of the skin-sensitive individuals were enrolled in the experiment, and the 45 volunteers were randomly divided into three groups of 15 individuals each, and half-face test control experiments were performed in groups of examples 16, 20, and 27 and comparative examples 1, 2, and 3, respectively.

2.2 sample preparation

Test samples: examples 16, 20, and 27 the resulting serums were prepared;

control sample: the essences prepared in comparative examples 1, 2 and 3 were respectively taken.

2.3 test methods

The test adopts a half-face control mode, one side uses a test sample, the other side uses a control sample, and the test sample is used in the morning and the evening. High-resolution photographs were taken before, 2 weeks, and 4 weeks after the use of the samples, respectively, and the amount of facial transdermal water loss and skin elasticity were measured.

2.4 instruments used

Skin elasticity tester MPA 580(Courage + Khazaka, Germany), and skin moisture loss tester Tewameter TM300(Courage + Khazaka, Germany)

2.5 test results

2.5.1 Effect of the serum on skin elasticity

In the design of the skin elasticity tester Cutometer Q software, R2 ═ Ua/Uf, where Ua denotes the recovery value of the skin from the removal of negative pressure to the next successive test of the skin surface plus negative pressure; uf represents the maximum amount of skin stretching for which there is a negative pressure.

The results are expressed as the ratio of the R2 value after application to the R2 value before application, with the initial value before application set to 100%, 2 weeks after application, the skin using the essences of examples 16, 20, 27 had an increase in elasticity of 13.32%, 12.56%, 10.78% compared to before application, and 24.56%, 25.37%, 12.35% after 4 weeks after application, while the increase in elasticity of the skin using comparative examples 1, 2, 3 was significantly lower than the test sample. The results are shown in Table 7.

TABLE 7 Effect of the essences on skin elasticity

	Before use	After 2 weeks of use	After 4 weeks of use
				Example 16	100.00％	113.32％	124.56％
Example 20	100.00％	112.56％	125.37％
				Example 27	100.00％	110.78	112.35％
Comparative example 1	100.00％	90.85％	91.26％
				Comparative example 2	100.00％	102.85％	104.75％
Comparative example 3	100.00％	106.24	108.36

2.5.2 Effect of the serum on skin moisture loss

The results were expressed as the ratio of the TEWL value after application to the TEWL value before application, and the percentage of moisture loss through the skin was significantly reduced in the essences of examples 16, 20, and 27 compared to the essences of comparative examples 1, 2, and 3 at 4 weeks after application, with the initial value before application set to 100%, indicating that the barrier function of the skin was enhanced. The results are shown in Table 8.

TABLE 8 Effect of the serum on skin moisture loss

	Before use	After 2 weeks of use	After 4 weeks of use
				Example 16	100.00％	92.85％	85.19％
Example 20	100.00％	92.61％	86.39％
				Example 27	100.00％	94.26％	87.31％
Comparative example 1	100.00％	98.25％	96.98％
				Comparative example 2	100.00％	97.87％	96.21％
Comparative example 3	100.00％	96.31％	95.13％

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. For the scheme disclosed by the embodiment, the scheme corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles disclosed herein.

Claims

1. An anti-aging composition containing polydeoxyribonucleic acid, which is characterized by comprising the following components: honey and polydeoxyribonucleic acid;

wherein the polydeoxyribonucleic acid is derived from the controlled purification and sterilization process of salmon sperm DNA;

the mass ratio of the honey to the polydeoxyribonucleic acid is (10-100): 1.

2. the polydeoxyribonucleic acid-containing anti-aging composition according to claim 1, wherein the honey is white honey.

3. A skin care product comprising an anti-aging composition comprising polydeoxyribonucleic acid, wherein the skin care product comprises the anti-aging composition of claim 1 or 2.

4. The skin care product of claim 3, wherein the skin care product further comprises cosmetically acceptable adjuvants.

5. The skin care product of claim 4, wherein the adjuvant comprises a moisturizer, a conditioning oil, an emulsifier, an acid-base regulator, a cosolvent, a thickener and/or a preservative.

6. The skin care product of claim 3, wherein the anti-aging composition comprises at least 0.5% by weight of the anti-aging composition.

7. The skin care product of claim 6, wherein the anti-aging composition comprises 0.5-2% by weight of the anti-aging composition.

8. The skin care product of claim 6 or 7, wherein the skin care product comprises a cream, essence, a stock solution or a mask.