CN112891624A - Preparation method of spinal cord regeneration and repair material - Google Patents
Preparation method of spinal cord regeneration and repair material Download PDFInfo
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- CN112891624A CN112891624A CN202110294508.7A CN202110294508A CN112891624A CN 112891624 A CN112891624 A CN 112891624A CN 202110294508 A CN202110294508 A CN 202110294508A CN 112891624 A CN112891624 A CN 112891624A
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- 210000000278 spinal cord Anatomy 0.000 title claims abstract description 34
- 230000008439 repair process Effects 0.000 title claims abstract description 23
- 239000000463 material Substances 0.000 title claims abstract description 20
- 230000008929 regeneration Effects 0.000 title claims abstract description 18
- 238000011069 regeneration method Methods 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000007787 solid Substances 0.000 claims abstract description 37
- 239000002002 slurry Substances 0.000 claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 230000010261 cell growth Effects 0.000 claims abstract description 8
- 239000003102 growth factor Substances 0.000 claims abstract description 7
- 210000001185 bone marrow Anatomy 0.000 claims abstract description 6
- 230000000149 penetrating effect Effects 0.000 claims abstract description 6
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 238000002145 thermally induced phase separation Methods 0.000 claims abstract description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 238000004132 cross linking Methods 0.000 claims description 11
- 239000003431 cross linking reagent Substances 0.000 claims description 10
- 229920001661 Chitosan Polymers 0.000 claims description 8
- 102000008186 Collagen Human genes 0.000 claims description 8
- 108010035532 Collagen Proteins 0.000 claims description 8
- AZKVWQKMDGGDSV-BCMRRPTOSA-N Genipin Chemical group COC(=O)C1=CO[C@@H](O)[C@@H]2C(CO)=CC[C@H]12 AZKVWQKMDGGDSV-BCMRRPTOSA-N 0.000 claims description 8
- 229920001436 collagen Polymers 0.000 claims description 8
- AZKVWQKMDGGDSV-UHFFFAOYSA-N genipin Natural products COC(=O)C1=COC(O)C2C(CO)=CCC12 AZKVWQKMDGGDSV-UHFFFAOYSA-N 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 102000004266 Collagen Type IV Human genes 0.000 claims description 2
- 108010042086 Collagen Type IV Proteins 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000001172 regenerating effect Effects 0.000 claims 5
- 208000020431 spinal cord injury Diseases 0.000 abstract description 12
- 210000004027 cell Anatomy 0.000 abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 230000035755 proliferation Effects 0.000 abstract description 4
- 230000012010 growth Effects 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 210000000225 synapse Anatomy 0.000 abstract description 3
- 230000004069 differentiation Effects 0.000 abstract description 2
- 230000007830 nerve conduction Effects 0.000 abstract description 2
- 210000002569 neuron Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000007849 functional defect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000000946 synaptic effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 102000001187 Collagen Type III Human genes 0.000 description 1
- 108010069502 Collagen Type III Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 210000003050 axon Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000005062 synaptic transmission Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dermatology (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a preparation method of a spinal cord regeneration and repair material, which comprises the following steps: step 1) manufacturing a mould according to the shape of the bone marrow, wherein the mould is a cylinder body which is communicated up and down; step 2), placing a plurality of filaments penetrating through two ends in the die; step 3), closing the lower end of the mold; step 4), injecting the biological slurry into the mold, putting the mold into a freeze dryer, and removing the solvent in the biological slurry through thermally induced phase separation to obtain a solid with a large number of micropores; and 5) taking the solid out of the cylinder and extracting the filaments to obtain the solid support with a large number of micropores and a plurality of axial channels. The spinal cord three-dimensional model manufactured by three-dimensional modeling can be better matched with the shape of the spinal cord injury part; the axial channel is consistent with the nerve conduction direction, which is beneficial to guiding the proliferation and differentiation of seed cells and establishing new synapse connection, and the micropores are used for retaining cell growth factors and promoting the proliferation and growth of the seed cells in the axial channel.
Description
Technical Field
The invention relates to a preparation method of a spinal cord regeneration and repair material.
Background
Spinal cord injury is a clinically common disease. Spinal cord injury is caused by a variety of causes, and can be caused by tumors, inflammation, trauma, etc. Spinal cord injury has a great impact on patients, most commonly, nerve function loss below the surface of spinal cord injury, and serious functional defects of a cardiovascular system, a respiratory system, a digestive system and the like can be caused. These functional defects all affect the normal life of the patient.
Spinal cord injury is not only destructive, but also difficult to recover because it is difficult to repair the neurotransmission pathway by synapse formation at full transection by own neurons following spinal cord injury. Therefore, until now, spinal cord regeneration repair has remained an important issue in clinical medicine.
At present, the best method for spinal cord regeneration and repair is to construct a degradable tissue engineering scaffold at the spinal cord injury, take the tissue engineering scaffold as a transplanted cell or a carrier of an active factor with the effects of protecting neurons and promoting axon regeneration, and form new neurons at the spinal cord injury by transplanting adult stem cells, induced pluripotent stem cells, embryonic stem cells and the like, thereby establishing synaptic connections and forming effective synaptic connections.
The existing spinal cord regeneration repair material has the following defects: 1. cannot form an accurate three-dimensional structure and cannot adapt to the shape of the spinal cord injury. 2. Micropores formed by the engineering scaffold are small and are not beneficial to the propagation and growth of cells.
Disclosure of Invention
The invention aims to provide a preparation method of a spinal cord regeneration repair material which can adapt to the shape of a spinal cord injury part and is suitable for cell propagation and growth aiming at the problems.
In order to achieve the aim, the invention discloses a preparation method of a spinal cord regeneration and repair material, which comprises the following steps: step 1) manufacturing a mould according to the shape of the bone marrow, wherein the mould is a cylinder body which is communicated up and down; step 2), placing a plurality of filaments penetrating through two ends in the die; step 3), closing the lower end of the mold; step 4), injecting the biological slurry into the mold, putting the mold into a freeze dryer, and removing the solvent in the biological slurry through thermally induced phase separation to obtain a solid with a large number of micropores; and 5) taking the solid out of the cylinder and extracting the filaments to obtain the solid support with a large number of micropores and a plurality of axial channels.
The step 1) comprises the following specific steps: step 1.1) designing a spinal cord three-dimensional model by using computer aided design software; and step 1.2) manufacturing a mold according to the spinal cord three-dimensional model.
The filaments in the step 2) are stainless steel wires.
The biological slurry in the step 4) is a mixture of collagen and chitosan.
The collagen is I, III or type IV collagen.
The preparation method of the biological slurry comprises the following steps: and 4.1) dissolving collagen and chitosan in a mass ratio of 2:1-1:1 in 0.05 mol/L acetic acid solution, and stirring at 800 rpm for 60 minutes in a constant temperature environment at 4 ℃.
The preparation method of the spinal cord regeneration and repair material also comprises the following steps: and 6) putting the solid scaffold obtained in the step 5 into a biological cross-linking agent for cross-linking and then freeze-drying.
The biological cross-linking agent is genipin solution.
The preparation method of the spinal cord regeneration and repair material further comprises the following steps: step 7) immersing the micropores in the solid support in cell growth factors.
The preparation method of the spinal cord regeneration and repair material further comprises the following steps: step 8) injecting seed cells into the axial channel in the solid support.
The invention has the beneficial effects that: the spinal cord three-dimensional model manufactured by three-dimensional modeling can be better matched with the shape of the spinal cord injury part; after the solid bracket made of collagen and chitosan is crosslinked, the biocompatibility and the mechanical property can reach higher level; the axial channel is consistent with the nerve conduction direction, which is beneficial to guiding the proliferation and differentiation of seed cells and establishing new synapse connection, and the micropores are used for retaining cell growth factors and promoting the proliferation and growth of the seed cells in the axial channel.
Detailed Description
Example 1
Step 1) manufacturing a mould according to the shape of the bone marrow, wherein the mould is a cylinder body which is communicated up and down. The step 1) comprises the following specific steps: step 1.1) designing a spinal cord three-dimensional model by using computer aided design software; and step 1.2) manufacturing a mold according to the spinal cord three-dimensional model.
And 2) placing a plurality of filaments penetrating through two ends in the mould, wherein the filaments are preferably stainless steel wires.
And 3) closing the lower end of the mold.
Step 4), injecting the biological slurry into the mold, putting the mold into a freeze dryer, and removing the solvent in the biological slurry through thermally induced phase separation to obtain a solid with a large number of micropores; the preparation method of the biological slurry comprises the following steps: and 4.1) dissolving the type I collagen and the chitosan with the mass ratio of 2:1 in 0.05 mol/L acetic acid solution, and stirring for 60 minutes at 800 rpm in a constant temperature environment at 4 ℃.
And 5) taking the solid out of the cylinder and extracting the filaments to obtain the solid support with a large number of micropores and a plurality of axial channels.
And 6) putting the solid scaffold obtained in the step 5 into a biological cross-linking agent for cross-linking and then freeze-drying. The biological cross-linking agent in this example is a genipin solution, and the cross-linking process includes: after immersing the solid scaffold in genipin solution with a concentration of 10 g/L for crosslinking for 48 hours, it was lyophilized at-40 ℃ for 24 hours.
Step 7) immersing the micropores in the solid support in cell growth factors.
Step 8) injecting seed cells into the axial channel in the solid support.
Example 2
Step 1) manufacturing a mould according to the shape of the bone marrow, wherein the mould is a cylinder body which is communicated up and down. The step 1) comprises the following specific steps: step 1.1) designing a spinal cord three-dimensional model by using computer aided design software; and step 1.2) manufacturing a mold according to the spinal cord three-dimensional model.
And 2) placing a plurality of filaments penetrating through two ends in the mould, wherein the filaments are preferably stainless steel wires.
And 3) closing the lower end of the mold.
Step 4), injecting the biological slurry into the mold, putting the mold into a freeze dryer, and removing the solvent in the biological slurry through thermally induced phase separation to obtain a solid with a large number of micropores; the preparation method of the biological slurry comprises the following steps: and 4.1) dissolving the type III collagen and the chitosan with the mass ratio of 3:2 into 0.05 mol/L acetic acid solution, and stirring for 60 minutes at 800 rpm in a constant temperature environment at 4 ℃.
And 5) taking the solid out of the cylinder and extracting the filaments to obtain the solid support with a large number of micropores and a plurality of axial channels.
And 6) putting the solid scaffold obtained in the step 5 into a biological cross-linking agent for cross-linking and then freeze-drying. The biological cross-linking agent in this example is a genipin solution, and the cross-linking process includes: after immersing the solid scaffold in genipin solution with a concentration of 10 g/L for crosslinking for 48 hours, it was lyophilized at-40 ℃ for 24 hours.
Step 7) immersing the micropores in the solid support in cell growth factors.
Step 8) injecting seed cells into the axial channel in the solid support.
Example 2
Step 1) manufacturing a mould according to the shape of the bone marrow, wherein the mould is a cylinder body which is communicated up and down. The step 1) comprises the following specific steps: step 1.1) designing a spinal cord three-dimensional model by using computer aided design software; and step 1.2) manufacturing a mold according to the spinal cord three-dimensional model.
And 2) placing a plurality of filaments penetrating through two ends in the mould, wherein the filaments are preferably stainless steel wires.
And 3) closing the lower end of the mold.
Step 4), injecting the biological slurry into the mold, putting the mold into a freeze dryer, and removing the solvent in the biological slurry through thermally induced phase separation to obtain a solid with a large number of micropores; the preparation method of the biological slurry comprises the following steps: and 4.1) dissolving the IV type collagen and the chitosan with the mass ratio of 1:1 in 0.05 mol/L acetic acid solution, and stirring for 60 minutes at 800 rpm in a constant temperature environment at 4 ℃.
And 5) taking the solid out of the cylinder and extracting the filaments to obtain the solid support with a large number of micropores and a plurality of axial channels.
And 6) putting the solid scaffold obtained in the step 5 into a biological cross-linking agent for cross-linking and then freeze-drying. The biological cross-linking agent in this example is a genipin solution, and the cross-linking process includes: after immersing the solid scaffold in genipin solution with a concentration of 10 g/L for crosslinking for 48 hours, it was lyophilized at-40 ℃ for 24 hours.
Step 7) immersing the micropores in the solid support in cell growth factors.
Step 8) injecting seed cells into the axial channel in the solid support.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (10)
1. A preparation method of a spinal cord regeneration and repair material is characterized by comprising the following steps:
step 1) manufacturing a mould according to the shape of the bone marrow, wherein the mould is a cylinder body which is communicated up and down;
step 2), placing a plurality of filaments penetrating through two ends in the die;
step 3), closing the lower end of the mold;
step 4), injecting the biological slurry into the mold, putting the mold into a freeze dryer, and removing the solvent in the biological slurry through thermally induced phase separation to obtain a solid with a large number of micropores;
and 5) taking the solid out of the cylinder and extracting the filaments to obtain the solid support with a large number of micropores and a plurality of axial channels.
2. The method for preparing a spinal cord regeneration repair material according to claim 1, wherein the step 1) comprises the following specific steps:
step 1.1) designing a spinal cord three-dimensional model by using computer aided design software;
and step 1.2) manufacturing a mold according to the spinal cord three-dimensional model.
3. The method for preparing a spinal cord regenerative repair material according to claim 1, wherein the filaments in step 2) are stainless steel wires.
4. The method for preparing a spinal cord regeneration repair material according to claim 1, wherein the bio-slurry in step 4) is a mixture of collagen and chitosan.
5. The method for preparing a material for the regenerative repair of the spinal cord according to claim 4, wherein said collagen is I, III or type IV collagen.
6. The method for preparing a spinal cord regeneration repair material according to claim 4, wherein the method for preparing the biological slurry comprises the steps of: and 4.1) dissolving collagen and chitosan in a mass ratio of 2:1-1:1 in 0.05 mol/L acetic acid solution, and stirring at 800 rpm for 60 minutes in a constant temperature environment at 4 ℃.
7. The method for preparing a spinal cord regenerative repair material according to claim 1, further comprising: and 6) putting the solid scaffold obtained in the step 5 into a biological cross-linking agent for cross-linking and then freeze-drying.
8. The method for preparing a spinal cord regeneration repair material according to claim 7, wherein the biological cross-linking agent is genipin solution.
9. The method for preparing a spinal cord regenerative repair material according to claim 7, further comprising: step 7) immersing the micropores in the solid support in cell growth factors.
10. The method for preparing a spinal cord regenerative repair material according to claim 7, further comprising: step 8) injecting seed cells into the axial channel in the solid support.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110294508.7A CN112891624A (en) | 2021-03-19 | 2021-03-19 | Preparation method of spinal cord regeneration and repair material |
| PCT/CN2021/120015 WO2022193599A1 (en) | 2021-03-19 | 2021-09-23 | Method for preparing spinal cord regeneration and repair material |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110294508.7A CN112891624A (en) | 2021-03-19 | 2021-03-19 | Preparation method of spinal cord regeneration and repair material |
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| CN112891624A true CN112891624A (en) | 2021-06-04 |
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| CN202110294508.7A Withdrawn CN112891624A (en) | 2021-03-19 | 2021-03-19 | Preparation method of spinal cord regeneration and repair material |
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| WO (1) | WO2022193599A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022193599A1 (en) * | 2021-03-19 | 2022-09-22 | 潍坊奥精医学研究有限公司 | Method for preparing spinal cord regeneration and repair material |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1303946C (en) * | 2004-06-25 | 2007-03-14 | 清华大学 | Nerve tissue engineering tube type bracket and method for making same |
| CN102671237B (en) * | 2009-01-16 | 2014-04-16 | 中国人民解放军第四军医大学 | High-simulation tissue engineering nerve-repair material and preparation method |
| CN101766836B (en) * | 2009-01-21 | 2012-09-05 | 丁坦 | Preparation method of nano silver cordspinal cord and peripheral nerve repairing material |
| EP2380601B1 (en) * | 2010-04-15 | 2013-03-20 | National University of Ireland, Galway | Multichannel collagen nerve conduit for nerve repair |
| CN102512266B (en) * | 2012-01-16 | 2015-04-29 | 杭州电子科技大学 | Method for preparing spinal cord injury repair tissue engineering stent |
| CN102688110A (en) * | 2012-06-13 | 2012-09-26 | 北京天新福医疗器材有限公司 | Multi-aperture nerve repairing tube and preparation method and application thereof |
| CN103263308B (en) * | 2013-05-17 | 2015-07-29 | 中国人民解放军第四军医大学 | Many micropores degradable collagen-chitin nerve trachea and preparation method thereof |
| US20190290283A1 (en) * | 2018-03-26 | 2019-09-26 | Wichita State University | Composite neural conduit |
| CN112891624A (en) * | 2021-03-19 | 2021-06-04 | 潍坊奥精医学研究有限公司 | Preparation method of spinal cord regeneration and repair material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022193599A1 (en) * | 2021-03-19 | 2022-09-22 | 潍坊奥精医学研究有限公司 | Method for preparing spinal cord regeneration and repair material |
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