CN112842944A - Cosmetic based on anti-aging collagen - Google Patents
Cosmetic based on anti-aging collagen Download PDFInfo
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- CN112842944A CN112842944A CN202110294316.6A CN202110294316A CN112842944A CN 112842944 A CN112842944 A CN 112842944A CN 202110294316 A CN202110294316 A CN 202110294316A CN 112842944 A CN112842944 A CN 112842944A
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- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 30
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- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 claims abstract description 18
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- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 claims abstract description 18
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- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 claims abstract description 18
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
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- CGSVCNBINHWWFW-UHFFFAOYSA-M dimethyl-(2-phenoxyethyl)-(thiophen-2-ylmethyl)azanium;chloride Chemical compound [Cl-].C=1C=CSC=1C[N+](C)(C)CCOC1=CC=CC=C1 CGSVCNBINHWWFW-UHFFFAOYSA-M 0.000 description 1
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Images
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/31—Hydrocarbons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Dermatology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Oil, Petroleum & Natural Gas (AREA)
- Emergency Medicine (AREA)
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- Cosmetics (AREA)
Abstract
The invention discloses an anti-aging collagen-based cosmetic which comprises the following raw materials in parts by weight: 30-40 parts of fish skin collagen, 40-50 parts of refined tea oil, 5-10 parts of sweet almond oil, 5-7 parts of caprylic/capric triglyceride, 1-3 parts of vitamin E, 1-3 parts of squalene, 0.5-1 part of tea tree essential oil, 50-70 parts of deionized water and 3-5 parts of an emulsifier; the method comprises the following steps: step S1, mixing refined tea oil, sweet almond oil, caprylic/capric triglyceride, vitamin E, squalene and tea tree essential oil to prepare mixed oil; step S2, dissolving fish skin collagen and an emulsifier in deionized water to prepare a collagen aqueous solution; s3, shearing and emulsifying the mixed oil and the collagen aqueous solution by using an oil-water mixing and emulsifying device to obtain the cosmetics in an emulsion state; the invention solves the problem that the existing anti-aging collagen cosmetics have poor absorption effect and anti-aging effect.
Description
Technical Field
The invention belongs to the technical field of anti-aging cosmetics, and particularly relates to a cosmetic based on anti-aging collagen.
Background
Although the aging is a natural phenomenon of life process, people can slow down the aging pace through some means, the life rhythm of people is accelerated, the working pressure is increased, the work and rest time is irregular, the skin color of the face is dark and dry, fine lines are easy to generate, the aging of the skin of the face is accelerated, and the anti-aging cosmetic is the cosmetic aiming at delaying the aging of the skin, so the anti-aging cosmetic has more urgent use requirements.
Collagen is a structural protein existing in extracellular matrix, and is widely distributed in animal connective tissue, and as a functional biological macromolecule, collagen has various characteristics, such as no cytotoxicity, controllable biomass degradation speed, capability of promoting or inhibiting interaction of cellular materials, excellent biochemical compatibility, and the like. At present, collagen in industrial production is mainly from cow leather, pigskin or bones, but the content of collagen in aquatic animals is higher than that of the aquatic animals, the content of collagen in fish skin can be up to 80% of the total protein content, and after the aquatic animals collagen is hydrolyzed, collagen hydrolysate with similar amino acid composition can be generated and is easily absorbed by the skin of human body.
However, the anti-aging collagen cosmetics sold on the market at present cannot be effectively absorbed by facial skin after being used, so that the anti-aging effect of the cosmetics cannot achieve the expected effect, and the use requirements of people cannot be met.
Disclosure of Invention
The invention aims to provide an anti-aging collagen-based cosmetic, and solves the problems that the anti-aging collagen cosmetic in the prior art cannot be effectively absorbed by facial skin and cannot achieve the expected anti-aging effect.
The purpose of the invention can be realized by the following technical scheme:
an anti-aging collagen-based cosmetic comprises the following raw materials in parts by weight: 30-40 parts of fish skin collagen, 40-50 parts of refined tea oil, 5-10 parts of sweet almond oil, 5-7 parts of caprylic/capric triglyceride, 1-3 parts of vitamin E, 1-3 parts of squalene, 0.5-1 part of tea tree essential oil, 50-70 parts of deionized water and 3-5 parts of an emulsifier;
the preparation method of the cosmetic based on the anti-aging collagen comprises the following steps:
step S1: heating refined tea oil in the formula weight part to 50-55 ℃, adding sweet almond oil and caprylic/capric triglyceride, stirring at a constant speed under the condition that the rotation speed is 80-100r/min, adding vitamin E and squalene after mixing uniformly, continuing to heat to 55-60 ℃, keeping the rotation speed constant, continuing to stir, cooling to 8-10 ℃ after the vitamin E and the squalene are completely dissolved, then adding tea tree essential oil, and stirring at a constant speed of 80-100r/min for 5-10min to obtain mixed oil;
step S2: adding the fish skin collagen in the weight part of the formula into half of deionized water, stirring at a constant speed of 100-120r/min for dissolving, adding an emulsifier and the other half of the deionized water, and stirring and mixing for 10-20min while keeping the rotating speed unchanged to obtain a collagen aqueous solution;
step S3: the method comprises the steps of adding a collagen aqueous solution and mixed oil into a barrel body from a feeding pipe of an oil-water mixing and emulsifying device respectively, starting a telescopic cylinder after a liquid level reaches the upper half part of the barrel body, extending a telescopic rod of the telescopic cylinder, driving a material sealing plate to move downwards by the telescopic rod of the telescopic cylinder, stopping the telescopic cylinder after the lower surface of the material sealing plate is contacted with the liquid level, starting a motor, driving an input end of a speed reducer to rotate by an output end of the motor, driving a stirring shaft to rotate by an output end of the speed reducer, rotating the stirring shaft at the rotating speed of 10000-15000r/min, driving a shearing rod to rotate by the stirring shaft, shearing and emulsifying the collagen aqueous solution and the mixed oil in the barrel body, wherein the stirring time is 10.
Further, the preparation method of the fish skin collagen specifically comprises the following steps:
step A1: removing scales, flesh and clean the fish skin which is preserved by freezing, shearing the fish skin into blocks of 1cm multiplied by 1cm, adding the fish skin blocks into a sodium hydroxide solution with the concentration of 0.1mol/L at the temperature of 4 ℃, stirring for 24-26h at the rotating speed of 200 plus one year at 400r/min, replacing the sodium hydroxide solution every 12h, washing the block fish skin treated by alkali liquor to be neutral by deionized water with the temperature of 4 ℃, and draining for later use;
step A2: adding the drained blocky fish skin into an ethanol solution with the mass fraction of 20%, carrying out heat preservation treatment at the temperature of 4 ℃ for 24 hours, removing lipid in the blocky fish skin, and removing the ethanol solution remained in the blocky fish skin by using deionized water to obtain fat-free blocky fish skin;
step A3: adding the fat-free block fish skin into an acetic acid solution with the concentration of 0.5mol/L, leaching for 48h at the temperature of 4 ℃, centrifuging for 30min at the rotation speed of 12000r/min 10000-;
step A4: and D, adding the collagen precipitate obtained in the step A3 into deionized water with the same mass, adding protease with the mass of 1 per thousand of that of the collagen precipitate into the deionized water, carrying out ultrasonic oscillation treatment for 2-4h under the condition that the pH value is 7-8 and the room temperature, centrifuging at the rotating speed of 10000-12000r/min to collect supernatant, dialyzing the supernatant in the deionized water by using a dialysis membrane until the pH value of the dialysis water is neutral, and concentrating, freezing and drying to obtain the fish skin collagen.
Furthermore, the fish skin is one or two of fresh water fish skin or ocean fish skin in any proportion.
Further, the dosage ratio of the blocky fish skin to the sodium hydroxide solution in the step A1 is 1g:10 mL; the dosage ratio of the drained blocky fish skin to the ethanol solution in the step A2 is 1g to 10 mL; the dosage ratio of the fat-free blocky fish skin to the acetic acid solution in the step A3 is 1g:10 mL.
Further, the refined tea oil is prepared by the following method:
placing tea oil in a conical flask, heating in water bath for 30-40min while stirring at 50-52 ℃ and at the rotation speed of 200-300r/min, transferring the hot tea oil into an ice-water bath for cooling, stirring at a constant speed of 80-100r/min, cooling to 10 ℃, stopping stirring to obtain cooled tea oil, transferring the cooled tea oil into a refrigerator at the temperature of 4 ℃, cooling for 24h for crystallization, and filtering to obtain the refined tea oil.
Further, the emulsifier is one or more of glycerol stearate, sucrose polystearate, polyglycerol-3-diisostearate, polyglycerol-6-distearate and polyglycerol-6-dioleate which are mixed in any proportion.
The oil-water mixing and emulsifying device comprises an emulsion barrel component, a sealing cover component and an emulsifying mechanism, wherein the sealing cover component is arranged above the emulsion barrel component, and the emulsifying mechanism is arranged in the center above the sealing cover component;
emulsion bucket subassembly includes staving, supporting legs, end socket, discharging pipe, bleeder valve and inlet pipe, the diameter of staving the latter half is less than the diameter of the first half, and the bottom installation of staving is fixed with three supporting legs, and is three the supporting legs uses staving bottom center to be circular array distribution as the centre of a circle, and the bottom center of staving runs through and installs the end socket, and one side bottom of staving is equipped with the discharging pipe, the one end and the staving outer wall fixed connection of discharging pipe, and discharging pipe and the inside intercommunication of staving install the bleeder valve on the discharging pipe, and one side top of staving is equipped with the inlet pipe, the one end and the staving outer wall fixed connection of inlet.
Further, sealed lid subassembly includes top cap, telescopic cylinder and a material sealing plate, the bottom of top cap and the top fixed connection of staving are equipped with two telescopic cylinders that opposition set up above the top cap, telescopic cylinder's cylinder body and top cap upper surface fixed connection, telescopic cylinder's telescopic link run through the top cap and with top cap sliding connection, telescopic cylinder's telescopic link bottom fixed connection has a material sealing plate, the material sealing plate is located the inside top of staving, and the week side and the staving inner wall of material sealing plate cooperate.
Furthermore, the center of the material sealing plate is provided with a shaft hole, a plurality of through holes are formed around the shaft hole, and the through holes are distributed in a circular array by taking the center of the material sealing plate as a circle center.
Further, emulsification mechanism includes motor, speed reducer, base, (mixing) shaft and shear bar, motor and speed reducer fixed connection, and the output of motor and the input fixed connection of speed reducer, the below installation of speed reducer is fixed with the base, fixed surface is connected on the bottom of base and the top cap, and the output installation of speed reducer is fixed with the (mixing) shaft, the bottom of (mixing) shaft runs through top cap and shaft hole and rotates with the end seat to be connected, (mixing) shaft and top cap and seal material board rotate to be connected, and the bottom installation of (mixing) shaft is fixed with a plurality of evenly distributed's shear bar.
The invention has the beneficial effects that:
the invention selects fresh water fish or deep sea fish skin with higher collagen content as the raw material of collagen, firstly, the impurities on the fish skin are removed through alkali washing treatment, then, the fish skin is soaked by ethanol, the lipid in the fish skin is removed, fat-free blocky fish skin is obtained, then, acetic acid and sodium chloride solution are used for extracting and separating out fish skin collagen precipitate, then, protease is used for decomposing the fish skin collagen precipitate with high molecular weight, and the fish skin collagen with lower molecular weight is obtained after dialysis concentration, so that the absorption and utilization of facial skin are more facilitated when in use;
the invention selects the tea oil as the base oil of the cosmetic, removes the lipid in the base oil through low-temperature preservation to obtain the refined tea oil, improves the acidity and the antioxidant activity of unsaturated fat of the base oil, and the refined tea oil is also an excellent percutaneous penetration enhancer, so that functional components in the cosmetic can effectively penetrate into the skin; the sweet almond oil is added, so that the viscosity of the cosmetic can be effectively reduced, and the cosmetic is fresh and cool without greasiness; by adding two functional components, namely vitamin E and squalene, the antioxidant performance of the cosmetic can be effectively improved, and the oxidative aging process of skin can be effectively slowed down; the tea tree essential oil is added, so that the fragrance of the cosmetic can be improved, and the cosmetic can also astringe the skin, sterilize and diminish inflammation; by using the oil-water mixing emulsifying device, the oil phase prepared by mixing the water phase containing the fish skin collagen and the emulsifying agent and the raw materials such as the tea oil and the like is sheared and emulsified, and the sealing plate which can move up and down is arranged to press the sealing plate to the liquid surface, so that the liquid surface and the air are isolated, the air entering the emulsion in the high-speed shearing and emulsifying process is reduced, and finally the emulsion-shaped cosmetic which is easy to be absorbed by the skin and has good anti-aging effect is prepared.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an oil-water mixing and emulsifying device according to the present invention;
FIG. 2 is a schematic cross-sectional view of an emulsion tank assembly of the present invention;
FIG. 3 is a front view of the oil-water mixing and emulsifying device of the present invention;
FIG. 4 is a schematic structural diagram of a material sealing plate according to the present invention;
fig. 5 is a schematic structural diagram of the emulsifying mechanism of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. an emulsion barrel assembly; 101. a barrel body; 102. supporting legs; 103. an end seat; 104. a discharge pipe; 105. a discharge valve; 106. a feed pipe; 2. sealing the cap assembly; 201. a top cover; 202. a telescopic cylinder; 203. a material sealing plate; 2031. a shaft hole; 2032. a through hole; 3. an emulsification mechanism; 301. a motor; 302. a speed reducer; 303. a base; 304. a stirring shaft; 305. and (4) shearing the rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An anti-aging collagen-based cosmetic comprises the following raw materials in parts by weight: 30 parts of fish skin collagen, 40 parts of refined tea oil, 5 parts of sweet almond oil, 5 parts of caprylic/capric triglyceride, 1 parts of vitamin E, 1 part of squalene, 0.5 part of tea tree essential oil, 50 parts of deionized water and 3 parts of emulsifier;
the preparation method of the cosmetic based on the anti-aging collagen comprises the following steps:
step S1: heating refined tea oil in parts by weight of the formula to 50 ℃, adding sweet almond oil and caprylic/capric triglyceride, stirring at a constant speed under the condition that the rotation speed is 80r/min, adding vitamin E and squalene after mixing uniformly, continuing to heat to 55 ℃, keeping the rotation speed unchanged, continuing to stir, cooling to 8 ℃ after the vitamin E and the squalene are completely dissolved, then adding tea tree essential oil, and stirring at a constant speed of 80r/min for 5min to obtain mixed oil;
step S2: adding fish skin collagen in the formula weight part into half of deionized water, stirring at a constant speed of 100r/min for dissolving, adding an emulsifier and the other half of deionized water, and stirring and mixing for 10min while keeping the rotating speed unchanged to obtain a collagen aqueous solution;
step S3: referring to fig. 1-5, a collagen aqueous solution and a mixed oil are respectively added into a barrel 101 from a feed pipe 106 of an oil-water mixing emulsification device, when a liquid level reaches the upper half part of the barrel 101, a telescopic cylinder 202 is started to extend a telescopic rod of the telescopic cylinder 202, the telescopic rod of the telescopic cylinder 202 drives a material sealing plate 203 to move downwards, when the lower surface of the material sealing plate 203 contacts with the liquid level, the telescopic cylinder 202 stops moving, a motor 301 is started, an output end of the motor 301 drives an input end of a speed reducer 302 to rotate, an output end of the speed reducer 302 drives a stirring shaft 304 to rotate, the stirring shaft 304 rotates at a rotation speed of 10000r/min, the stirring shaft 304 drives a shearing rod 305 to rotate, the collagen aqueous solution and the mixed oil inside the barrel 101 are sheared and emulsified, and the stirring time is 10min, so that a.
The preparation method of the fish skin collagen specifically comprises the following steps:
step A1: removing scales, flesh and water from the fish skin which is preserved by freezing, cutting the fish skin into blocks of 1cm multiplied by 1cm, adding the fish skin blocks into a sodium hydroxide solution with the concentration of 0.1mol/L at the temperature of 4 ℃, stirring for 24 hours at the rotating speed of 200r/min, replacing the sodium hydroxide solution every 12 hours, washing the fish skin blocks treated by alkali liquor to be neutral by deionized water with the temperature of 4 ℃, and draining for later use;
step A2: adding the drained blocky fish skin into an ethanol solution with the mass fraction of 20%, carrying out heat preservation treatment at the temperature of 4 ℃ for 24 hours, removing lipid in the blocky fish skin, and removing the ethanol solution remained in the blocky fish skin by using deionized water to obtain fat-free blocky fish skin;
step A3: adding the fat-free block fish skin into an acetic acid solution with the concentration of 0.5mol/L, leaching for 48h at the temperature of 4 ℃, centrifuging for 30min at the rotating speed of 10000r/min after leaching, taking supernatant, adding the supernatant into a sodium chloride solution with the final concentration of 0.9mol/L, standing overnight at the temperature of 4 ℃ to precipitate the collagen of the fish skin, and centrifuging for 30min at the rotating speed of 10000r/min to obtain collagen precipitate;
step A4: and D, adding the collagen precipitate obtained in the step A3 into deionized water with the same mass, then adding protease with the mass of 1 per mill of the collagen precipitate into the deionized water, carrying out ultrasonic oscillation treatment for 2h under the condition that the pH value is 7 and the room temperature, centrifuging at the rotating speed of 10000r/min, collecting supernatant, dialyzing the supernatant in the deionized water by using a dialysis membrane until the pH value of dialysis water is neutral, concentrating, freezing and drying to obtain the fish skin collagen.
The fish skin is one or two of fresh water fish skin or marine fish skin in any proportion.
The dosage ratio of the blocky fish skin to the sodium hydroxide solution in the step A1 is 1g to 10 mL; the dosage ratio of the drained blocky fish skin to the ethanol solution in the step A2 is 1g to 10 mL; the dosage ratio of the fat-free blocky fish skin to the acetic acid solution in the step A3 is 1g:10 mL.
The refined tea oil is prepared by the following method:
placing tea oil in a conical flask, heating in water bath under stirring at 50 deg.C and 200r/min for 30min, cooling hot tea oil in ice water bath, stirring at 80r/min, cooling to 10 deg.C, stopping stirring to obtain cooled tea oil, transferring the cooled tea oil into a refrigerator at 4 deg.C, cooling for 24 hr for crystallization, and filtering to obtain refined tea oil.
The emulsifier is glyceryl stearate.
Example 2
An anti-aging collagen-based cosmetic comprises the following raw materials in parts by weight: 35 parts of fishskin collagen, 45 parts of refined tea oil, 8 parts of sweet almond oil, 6 parts of caprylic/capric triglyceride, 2 parts of vitamin E, 2 parts of squalene, 0.5 part of tea tree essential oil, 60 parts of deionized water and 4 parts of emulsifier;
the preparation method of the cosmetic based on the anti-aging collagen comprises the following steps:
step S1: heating refined tea oil in parts by weight of the formula to 52 ℃, adding sweet almond oil and caprylic/capric triglyceride, stirring at a constant speed under the condition that the rotation speed is 90r/min, adding vitamin E and squalene after mixing uniformly, continuing to heat to 58 ℃, keeping the rotation speed unchanged, continuing to stir, cooling to 9 ℃ after the vitamin E and the squalene are completely dissolved, then adding tea tree essential oil, and stirring at a constant speed of 90r/min for 7min to obtain mixed oil;
step S2: adding fish skin collagen in the formula weight portion into half of deionized water, stirring at a constant speed of 110r/min for dissolving, adding an emulsifier and the other half of deionized water, and stirring and mixing for 15min while keeping the rotating speed unchanged to obtain a collagen aqueous solution;
step S3: referring to fig. 1-5, a collagen aqueous solution and a mixed oil are respectively added into a barrel 101 from a feed pipe 106 of an oil-water mixing emulsification device, when a liquid level reaches the upper half part of the barrel 101, a telescopic cylinder 202 is started to extend a telescopic rod of the telescopic cylinder 202, the telescopic rod of the telescopic cylinder 202 drives a material sealing plate 203 to move downwards, when the lower surface of the material sealing plate 203 contacts with the liquid level, the telescopic cylinder 202 stops moving, a motor 301 is started, an output end of the motor 301 drives an input end of a speed reducer 302 to rotate, an output end of the speed reducer 302 drives a stirring shaft 304 to rotate, the stirring shaft 304 rotates at a rotating speed of 12000r/min, the stirring shaft 304 drives a shearing rod 305 to rotate, the collagen aqueous solution and the mixed oil inside the barrel 101 are emulsified and sheared, and the stirring time is 12min, so that a.
The preparation method of the fish skin collagen specifically comprises the following steps:
step A1: removing scales, flesh and water from the fish skin which is preserved by freezing, cutting the fish skin into blocks of 1cm multiplied by 1cm, adding the fish skin blocks into a sodium hydroxide solution with the concentration of 0.1mol/L at the temperature of 4 ℃, stirring for 25 hours at the rotating speed of 300r/min, replacing the sodium hydroxide solution every 12 hours, washing the fish skin blocks treated by alkali liquor to be neutral by deionized water with the temperature of 4 ℃, and draining for later use;
step A2: adding the drained blocky fish skin into an ethanol solution with the mass fraction of 20%, carrying out heat preservation treatment at the temperature of 4 ℃ for 24 hours, removing lipid in the blocky fish skin, and removing the ethanol solution remained in the blocky fish skin by using deionized water to obtain fat-free blocky fish skin;
step A3: adding the fat-free blocky fish skin into an acetic acid solution with the concentration of 0.5mol/L, leaching for 48h at the temperature of 4 ℃, centrifuging for 30min at the rotating speed of 11000r/min after leaching, taking supernatant, adding the supernatant into a sodium chloride solution with the final concentration of 0.9mol/L, standing overnight at the temperature of 4 ℃ to precipitate the collagen of the fish skin, and centrifuging for 30min at the rotating speed of 11000r/min to obtain collagen precipitate;
step A4: and D, adding the collagen precipitate obtained in the step A3 into deionized water with the same mass, then adding protease with the mass of 1 per mill of the collagen precipitate into the deionized water, carrying out ultrasonic oscillation treatment for 3h under the condition of pH value of 7 and room temperature, centrifuging at the rotating speed of 11000r/min, collecting supernatant, dialyzing the supernatant in the deionized water by using a dialysis membrane until the pH value of dialysis water is neutral, concentrating, and freeze-drying to obtain the fish skin collagen.
The fish skin is one or two of fresh water fish skin or marine fish skin in any proportion.
The dosage ratio of the blocky fish skin to the sodium hydroxide solution in the step A1 is 1g to 10 mL; the dosage ratio of the drained blocky fish skin to the ethanol solution in the step A2 is 1g to 10 mL; the dosage ratio of the fat-free blocky fish skin to the acetic acid solution in the step A3 is 1g:10 mL.
The refined tea oil is prepared by the following method:
placing tea oil in a conical flask, heating in water bath under stirring at 250r/min and 51 deg.C for 35min, cooling hot tea oil in ice water bath, stirring at 90r/min, cooling to 10 deg.C, stopping stirring to obtain cooled tea oil, transferring the cooled tea oil into a refrigerator at 4 deg.C, cooling for 24 hr for crystallization, and filtering to obtain refined tea oil.
The emulsifier is prepared by mixing glyceryl stearate and sucrose polystearate according to the proportion of 1: 1.
Example 3
An anti-aging collagen-based cosmetic comprises the following raw materials in parts by weight: 40 parts of fish skin collagen, 50 parts of refined tea oil, 10 parts of sweet almond oil, 7 parts of caprylic/capric triglyceride, 3 parts of vitamin E, 3 parts of squalene, 1 part of tea tree essential oil, 70 parts of deionized water and 5 parts of an emulsifier;
the preparation method of the cosmetic based on the anti-aging collagen comprises the following steps:
step S1: heating refined tea oil in parts by weight of the formula to 55 ℃, adding sweet almond oil and caprylic/capric triglyceride, stirring at a constant speed under the condition that the rotation speed is 100r/min, adding vitamin E and squalene after mixing uniformly, continuing to heat to 60 ℃, keeping the rotation speed unchanged, continuing to stir, cooling to 10 ℃ after the vitamin E and the squalene are completely dissolved, then adding tea tree essential oil, and stirring at a constant speed of 100r/min for 10min to obtain mixed oil;
step S2: adding fish skin collagen in the formula weight portion into half of deionized water, stirring at a constant speed of 120r/min for dissolving, adding an emulsifier and the other half of deionized water, and stirring and mixing for 20min while keeping the rotating speed unchanged to obtain a collagen aqueous solution;
step S3: referring to fig. 1-5, a collagen aqueous solution and a mixed oil are respectively added into a barrel 101 from a feed pipe 106 of an oil-water mixing emulsification device, when a liquid level reaches the upper half of the barrel 101, a telescopic cylinder 202 is started to extend a telescopic rod of the telescopic cylinder 202, the telescopic rod of the telescopic cylinder 202 drives a material sealing plate 203 to move downwards, when the lower surface of the material sealing plate 203 contacts with the liquid level, the telescopic cylinder 202 stops moving, a motor 301 is started, an output end of the motor 301 drives an input end of a speed reducer 302 to rotate, an output end of the speed reducer 302 drives a stirring shaft 304 to rotate, the stirring shaft 304 rotates at a rotating speed of 15000r/min, the stirring shaft 304 drives a shearing rod 305 to rotate, the collagen aqueous solution and the mixed oil inside the barrel 101 are emulsified and are sheared, and the stirring time is 15min, so that a.
The preparation method of the fish skin collagen specifically comprises the following steps:
step A1: removing scales, flesh and water from the fish skin which is preserved by freezing, cutting the fish skin into blocks of 1cm multiplied by 1cm, adding the fish skin blocks into a sodium hydroxide solution with the concentration of 0.1mol/L at the temperature of 4 ℃, stirring for 26 hours at the rotating speed of 400r/min, replacing the sodium hydroxide solution every 12 hours, washing the fish skin blocks treated by alkali liquor to be neutral by deionized water with the temperature of 4 ℃, and draining for later use;
step A2: adding the drained blocky fish skin into an ethanol solution with the mass fraction of 20%, carrying out heat preservation treatment at the temperature of 4 ℃ for 24 hours, removing lipid in the blocky fish skin, and removing the ethanol solution remained in the blocky fish skin by using deionized water to obtain fat-free blocky fish skin;
step A3: adding the fat-free blocky fish skin into an acetic acid solution with the concentration of 0.5mol/L, leaching for 48h at the temperature of 4 ℃, centrifuging for 30min at the rotating speed of 12000r/min after leaching, taking supernatant, adding the supernatant into a sodium chloride solution with the final concentration of 0.9mol/L, standing overnight at the temperature of 4 ℃ to precipitate the collagen of the fish skin, and centrifuging for 30min at the rotating speed of 12000r/min to obtain collagen precipitate;
step A4: and D, adding the collagen precipitate obtained in the step A3 into deionized water with the same mass, then adding protease with the mass of 1 per mill of the collagen precipitate into the deionized water, carrying out ultrasonic oscillation treatment for 4h at the pH value of 8 and at the room temperature, centrifuging at the rotating speed of 12000r/min, collecting supernatant, dialyzing the supernatant in the deionized water by using a dialysis membrane until the pH value of dialysis water is neutral, concentrating, and freeze-drying to obtain the fish skin collagen.
The fish skin is one or two of fresh water fish skin or marine fish skin in any proportion.
The dosage ratio of the blocky fish skin to the sodium hydroxide solution in the step A1 is 1g to 10 mL; the dosage ratio of the drained blocky fish skin to the ethanol solution in the step A2 is 1g to 10 mL; the dosage ratio of the fat-free blocky fish skin to the acetic acid solution in the step A3 is 1g:10 mL.
The refined tea oil is prepared by the following method:
placing tea oil in a conical flask, heating in water bath under stirring at 52 deg.C and 300r/min for 40min, transferring hot tea oil into ice water bath for cooling, stirring at 100r/min, cooling to 10 deg.C, stopping stirring to obtain cooled tea oil, transferring the cooled tea oil into a refrigerator at 4 deg.C, cooling for crystallization for 24 hr, and filtering to obtain refined tea oil.
The emulsifier is one or more of glyceryl stearate, sucrose polystearate, polyglycerol-3-diisostearate, polyglycerol-6-distearate and polyglycerol-6-dioleate which are mixed in any proportion.
Referring to fig. 1-5, the oil-water mixing and emulsifying device in the above embodiment includes an emulsion barrel assembly 1, a sealing cover assembly 2 and an emulsifying mechanism 3, wherein the sealing cover assembly 2 is installed above the emulsion barrel assembly 1, and the emulsifying mechanism 3 is installed at the center above the sealing cover assembly 2;
Sealed lid subassembly 2 includes top cap 201, telescopic cylinder 202 and seals flitch 203, the bottom of top cap 201 and the top fixed connection of staving 101 are equipped with two telescopic cylinder 202 that opposition set up above top cap 201, telescopic cylinder 202's cylinder body and top cap 201 upper surface fixed connection, telescopic cylinder 202's telescopic link run through top cap 201 and with top cap 201 sliding connection, telescopic cylinder 202's telescopic link bottom fixedly connected with seals flitch 203, seal flitch 203 is located the inside top of staving 101, and all sides of seals flitch 203 cooperate with staving 101 inner wall.
The center of the material sealing plate 203 is provided with a shaft hole 2031, a plurality of through holes 2032 are arranged around the shaft hole 2031, and the through holes 2032 are distributed in a circular array by taking the center of the material sealing plate 203 as a circle center.
The working principle of the invention is as follows:
when the oil-water mixing emulsifying device is used, oil phase and water phase to be mixed and emulsified are firstly respectively introduced into the barrel body 101 through the feeding pipe 106, when the liquid level reaches the upper half part of the barrel body 101, the telescopic cylinder 202 is started, the telescopic rod of the telescopic cylinder 202 extends, the telescopic rod of the telescopic cylinder 202 drives the material sealing plate 203 to move downwards, when the lower surface of the material sealing plate 203 is contacted with the liquid level, the telescopic cylinder 202 stops acting, the motor 301 is started, the output end of the motor 301 drives the input end of the speed reducer 302 to rotate, the output end of the speed reducer 302 drives the stirring shaft 304 to rotate, the stirring shaft 304 drives the shearing rod 305 to rotate at a high speed, the oil phase and the water phase in the barrel body 101 are sheared and emulsified.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (6)
1. An anti-aging collagen-based cosmetic characterized by: the composite material comprises the following raw materials in parts by weight: 30-40 parts of fish skin collagen, 40-50 parts of refined tea oil, 5-10 parts of sweet almond oil, 5-7 parts of caprylic/capric triglyceride, 1-3 parts of vitamin E, 1-3 parts of squalene, 0.5-1 part of tea tree essential oil, 50-70 parts of deionized water and 3-5 parts of an emulsifier;
the preparation method of the cosmetic based on the anti-aging collagen comprises the following steps:
step S1: heating refined tea oil in the formula weight part to 50-55 ℃, adding sweet almond oil and caprylic/capric triglyceride, stirring at a constant speed under the condition that the rotation speed is 80-100r/min, adding vitamin E and squalene after mixing uniformly, continuing to heat to 55-60 ℃, keeping the rotation speed constant, continuing to stir, cooling to 8-10 ℃ after the vitamin E and the squalene are completely dissolved, then adding tea tree essential oil, and stirring at a constant speed of 80-100r/min for 5-10min to obtain mixed oil;
step S2: adding the fish skin collagen in the weight part of the formula into half of deionized water, stirring at a constant speed of 100-120r/min for dissolving, adding an emulsifier and the other half of the deionized water, and stirring and mixing for 10-20min while keeping the rotating speed unchanged to obtain a collagen aqueous solution;
step S3: respectively adding the collagen aqueous solution and the mixed oil into an oil-water mixing and emulsifying device, and shearing and emulsifying the collagen aqueous solution and the mixed oil for 10-15min to obtain the cosmetic in an emulsion state.
2. The cosmetic based on anti-aging collagen according to claim 1, wherein: the preparation method of the fish skin collagen specifically comprises the following steps:
step A1: removing scales, flesh and clean the fish skin which is preserved by freezing, shearing the fish skin into blocks of 1cm multiplied by 1cm, adding the fish skin blocks into a sodium hydroxide solution with the concentration of 0.1mol/L at the temperature of 4 ℃, stirring for 24-26h at the rotating speed of 200 plus one year at 400r/min, replacing the sodium hydroxide solution every 12h, washing the block fish skin treated by alkali liquor to be neutral by deionized water with the temperature of 4 ℃, and draining for later use;
step A2: adding the drained blocky fish skin into an ethanol solution with the mass fraction of 20%, carrying out heat preservation treatment at the temperature of 4 ℃ for 24 hours, removing lipid in the blocky fish skin, and removing the ethanol solution remained in the blocky fish skin by using deionized water to obtain fat-free blocky fish skin;
step A3: adding the fat-free block fish skin into an acetic acid solution with the concentration of 0.5mol/L, leaching for 48h at the temperature of 4 ℃, centrifuging for 30min at the rotation speed of 12000r/min 10000-;
step A4: and D, adding the collagen precipitate obtained in the step A3 into deionized water with the same mass, adding protease with the mass of 1 per thousand of that of the collagen precipitate into the deionized water, carrying out ultrasonic oscillation treatment for 2-4h under the condition that the pH value is 7-8 and the room temperature, centrifuging at the rotating speed of 10000-12000r/min to collect supernatant, dialyzing the supernatant in the deionized water by using a dialysis membrane until the pH value of the dialysis water is neutral, and concentrating, freezing and drying to obtain the fish skin collagen.
3. The cosmetic based on anti-aging collagen according to claim 2, wherein: the fish skin is one or two of fresh water fish skin or marine fish skin in any proportion.
4. The cosmetic based on anti-aging collagen according to claim 2, wherein: the dosage ratio of the blocky fish skin to the sodium hydroxide solution in the step A1 is 1g to 10 mL; the dosage ratio of the drained blocky fish skin to the ethanol solution in the step A2 is 1g to 10 mL; the dosage ratio of the fat-free blocky fish skin to the acetic acid solution in the step A3 is 1g:10 mL.
5. The cosmetic based on anti-aging collagen according to claim 1, wherein: the refined tea oil is prepared by the following method:
placing tea oil in a conical flask, heating in water bath for 30-40min while stirring at 50-52 ℃ and at the rotation speed of 200-300r/min, transferring the hot tea oil into an ice-water bath for cooling, stirring at a constant speed of 80-100r/min, cooling to 10 ℃, stopping stirring to obtain cooled tea oil, transferring the cooled tea oil into a refrigerator at the temperature of 4 ℃, cooling for 24h for crystallization, and filtering to obtain the refined tea oil.
6. The cosmetic based on anti-aging collagen according to claim 1, wherein: the emulsifier is one or more of glyceryl stearate, sucrose polystearate, polyglycerol-3-diisostearate, polyglycerol-6-distearate and polyglycerol-6-dioleate which are mixed in any proportion.
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| CN202110294316.6A CN112842944A (en) | 2021-03-19 | 2021-03-19 | Cosmetic based on anti-aging collagen |
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| CN202110294316.6A CN112842944A (en) | 2021-03-19 | 2021-03-19 | Cosmetic based on anti-aging collagen |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113332217A (en) * | 2021-05-31 | 2021-09-03 | 美卓(杭州)医疗科技有限公司 | Composite collagen composition for repairing neck shrinkage and sagging |
| CN114010767A (en) * | 2021-11-04 | 2022-02-08 | 宋东雪 | Slimming and shaping product, preparation equipment and method thereof, and application of slimming and shaping product in fat reducing effect |
| CN116003577A (en) * | 2023-02-14 | 2023-04-25 | 广东丸美生物技术股份有限公司 | Recombinant collagen for skin soothing and repairing and application thereof |
-
2021
- 2021-03-19 CN CN202110294316.6A patent/CN112842944A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113332217A (en) * | 2021-05-31 | 2021-09-03 | 美卓(杭州)医疗科技有限公司 | Composite collagen composition for repairing neck shrinkage and sagging |
| CN114010767A (en) * | 2021-11-04 | 2022-02-08 | 宋东雪 | Slimming and shaping product, preparation equipment and method thereof, and application of slimming and shaping product in fat reducing effect |
| CN116003577A (en) * | 2023-02-14 | 2023-04-25 | 广东丸美生物技术股份有限公司 | Recombinant collagen for skin soothing and repairing and application thereof |
| CN116003577B (en) * | 2023-02-14 | 2023-10-31 | 广东丸美生物技术股份有限公司 | Recombinant collagen for skin soothing and repairing and application thereof |
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