CN112813196B - Capture probe set, method, kit and application for detecting pathogenic microorganisms - Google Patents
Capture probe set, method, kit and application for detecting pathogenic microorganisms Download PDFInfo
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Abstract
The invention relates to a capture probe set, a method, a kit and application for detecting pathogenic microorganisms, and belongs to the technical field of gene detection. The capture probe group can specifically detect 62 bacteria and 41 drug-resistant genes, 16 fungi, 14 parasites, 15 DNA viruses and 60 RNA viruses, can cover more than 95% of pathogenic microorganisms with higher detection rate in clinical samples, combines the current situation that part of pathogenic microorganisms are difficult to detect, can greatly improve detection sensitivity and specificity, and particularly improves detection sensitivity of pathogenic microorganisms with clinical significance.
Description
Technical Field
The invention relates to the technical field of gene detection, in particular to a capture probe set, a method, a kit and application for detecting pathogenic microorganisms.
Background
Microbial pathogen detection has been an important issue in the diagnosis of infection. Microbial culture has long remained the gold standard for pathogen detection. Clinical applications also include antigen, antibody detection, PCR detection, staining, smear, microscopy, etc. However, the long time and low positive rate are drawbacks of the conventional detection method, and are also the main reasons for the difficulty of clinical diagnosis.
In recent years, the development of metagenomic next-generation sequencing (mNGS) provides a new idea for pathogen detection. Metagenome, also referred to as the environmental genome of a pathogenic microorganism or the genome of a pathogenic microorganism, refers to the sum of the genetic material of all microorganisms in a particular tissue or sample, and includes the genome of microorganisms that are currently culturable and incapable of cultivation in vitro. The metagenome can detect all sequences including bacteria, fungi, viruses and parasites in biological samples at one time by virtue of its no preference, rapidity (24 h) and high sensitivity. However, as a new detection method, there are many problems of metagenome, such as: because of the characteristics of a biological sample with extremely high human host ratio and low microorganism content of mNSS, sequencing data are mostly artificial, so that real pathogens can be covered by a large number of human sequences, the sensitivity is low, false negative is easy to cause, the specificity is not strong, a large number of background microorganisms are easy to bring, the clinical positive coincidence rate of the biological sample is required to be improved, and the like; in metagenome sequencing, the requirement on the detection data amount is high because the signal of the pathogenic microorganism sequence is extremely low, and the sequencing cost is increased.
Currently, for macro-transcriptome detection, the most effective method for removing human sequences is to digest human genomic DNA by using DNase and consume a large amount of human ribosomal RNA sequences by RNaseH method, so as to achieve the purpose of host removal. While for various human host removal protocols by DNA libraries, there are shortcomings: if the methylation sites of the human nucleic acid and the microorganism nucleic acid exist or not, the specific methylation antibody is used for removing the human nucleic acid containing the methylation sites, the enrichment efficiency is only 3-5 times, and the effect is not obvious. Another method for removing hosts is to utilize the difference of the cracking difficulty of human cells and microbial cells, release human genome nucleic acid after the difference of the cracking of human cells, and digest by using dnase, so that the enrichment efficiency of some microorganisms can reach more than 1000 times, but for microorganisms such as mycoplasma, parasites or microorganisms existing in the form of cfDNA without cell walls in a sample, the method can cause pathogen loss and risk of false negative results.
Therefore, the existing multiple host removal methods have defects, and a detection method with extremely high sensitivity is needed to improve the detection capability of the metagenome and the clinical positive coincidence rate.
Meanwhile, the plasma sample is commonly used for metagenome detection at present, and microorganisms with larger sedimentation coefficients such as bacteria and fungi are easy to leak detection, and if whole blood is detected, the human host is too high due to the fact that the white blood cell ratio in the whole blood sample is too high, the detection result of microorganisms with low abundance is not ideal, and false negative results are often caused. The two detection methods have errors with the results of clinical gold standard blood culture, so that a detection method capable of ensuring that all types of pathogenic microorganisms can be detected is needed, and the method has high sensitivity so as to reduce the influence of human hosts on the detection results.
In addition to the removal of the human host, multiplex PCR or liquid phase hybridization capture methods may be used to forward enrich the pathogenic microorganisms, thereby increasing sensitivity. Although the former has short time, the former has the defects of difficult design, small target range and the like; the latter, although having relevant applications in the tumor field, has far greater complexity in the variety and sequence of pathogenic microorganisms than human exons and tumor and cancer genes, making the design of probe libraries for detection of pathogenic microorganisms more difficult. Whereas the commercially available libraries of probes for liquid phase hybridization capture for detection of pathogenic microorganisms are often directed against one, or a few, pathogens. The highly preferential probe pool limits mNSS detection in clinical samples of numerous pathogenic microorganisms, which is detrimental to "unbiased" mNSS pathogen detection.
How to design a high-specificity targeting capture probe aiming at a wide range of pathogens and species of the same genus so as to accurately detect different pathogenic microorganisms is a great difficulty in the application of the liquid phase hybridization capture method in the pathogenic field. Therefore, there is no product for broad-spectrum pathogen detection by using the technology in the prior art, and often the detection of specific diseases or individual pathogens is performed, and no hybridization capture detection product which is applicable to mNGS and faces different sample types, disease types and pathogen types and has high sensitivity is established.
Disclosure of Invention
Based on the above, it is necessary to provide a capture probe set for detecting pathogenic microorganisms, which uses a liquid phase hybridization capture technology to perform targeted capture of pathogenic microorganisms on a conventional mNGS library, and is capable of making the detection sensitivity of pathogenic microorganisms higher, the specificity higher, and the advantage of having a strong detection signal, which is beneficial to the clinical detection of mNGS.
A set of capture probes for detecting a pathogenic microorganism, the set of capture probes specifically capturing the following pathogenic microorganism genomic coding region sequences: dengue virus 1, dengue virus 2, dengue virus 3, dengue virus 4, human bocavirus 1, human bocavirus 2, human bocavirus 3, human bocavirus 4, influenza a virus H10N1, influenza a virus H1N1, influenza a virus H2N2, influenza a virus H3N2, influenza a virus H5N1, influenza a virus H7N2, influenza a virus H7N7, influenza a virus H9N2, influenza B virus, human adenovirus, human coronavirus 229E, human coronavirus HKU1, human coronavirus NL63, human coronavirus OC43, human respiratory virus type 1 (human parainfluenza a virus type 1), human respiratory virus type 3 (human parainfluenza virus type 3), human mumps virus type 2 (human parainfluenza virus type 2), human mumps virus type 4 (human parainfluenza virus type 4), human respiratory syncytial virus type A, human respiratory syncytial virus type B, rhinovirus type A, rhinovirus type B, rhinovirus type C, enterovirus, human metapneumovirus, human herpesvirus type 1 (HSV 1), human herpesvirus type 2 (HSV 2), human herpesvirus type 3 (VZV), human herpesvirus type 5 (CMV), human herpesvirus type 6A, human herpesvirus type 6B, human herpesvirus type 7, human herpesvirus type 4 (EBV), human herpesvirus type 8, human parareovirus type 1, human paraenterovirus type 2, human parareovirus type 3, japanese encephalitis virus, swine herpesvirus, human parvovirus B19, influenza C virus, influenza virus type D, enterococcus faecium, enterococcus faecalis, acinetobacter baumannii, serratia marcescens, proteus vulgaris, proteus mirabilis, klebsiella pneumoniae, klebsiella aerogenes, enterobacter cloacae, escherichia coli, haemophilus influenzae, moraxella catarrhalis, pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella oxytoca, streptococcus pyogenes, legionella pneumophila, mycoplasma pneumoniae, chlamydia pneumoniae, listeria monocytogenes, neisseria meningitides, bordetella pertussis, mycobacterium abscessus, mycobacterium tuberculosis, mycobacterium intracellulare, mycobacterium kansasii, mycobacterium avium, mycobacterium marini, haemophilus parainfluenza, vibrio parahaemolyticus, vibrio vulnificus, leucomatous, M.maltophilii, chlamydia psittaci, lactobacillus delbrueckii, mycoplasma hominum, rickettsia prairii, eastern body of johnsonia, nocardia brasiliensis, nocardia guerbeta, nocardia meldonii, nocardia guinea, clostridium perfringens, streptococcus agalactiae, morganella morganii, corynebacterium diphtheriae, salmonella enterica, brucella canis, candida albicans, candida glabrata, pichia kudri, candida parapsilosis, candida tropicalis, cryptococcus neoformans, cryptococcus garter, aspergillus fumigatus, aspergillus flavus, aspergillus niger, aspergillus nidulans, aspergillus terreus, yersinia pneumospori, penicillium marneffei, round-wire-like manure, guano, C Yan Huan sporococci, trichomonas vaginalis, toxoplasma gondii, taenia tenacissima, baboon b amab, hepatitis GB type C, human polyomavirus type 2 (JC polyomavirus), human polyomavirus type 1 (BK polyomavirus), hantavirus, rubella virus, novel bunyavirus, clostridium tetani, cerdospora spinosa, histoplasmosis capsulata, chlamydia trachomatis, 2019 novel coronavirus, human immunodeficiency virus type 1, human immunodeficiency virus type 2, rabies virus, human poliovirus type 1, zipraecox virus, human hepatitis a virus, hepatitis B virus, measles virus, neisseria gonorrhoeae, treponema pallidum, yersinia pestis, bacillus anthracis, mycobacterium leprae, schistosoma japonicum, schistosoma mansoni, plasmodium malariae, amoebola, amoeba histolytica, leishmania, mycobacterium fortunai, echinococcus, human polyoma virus type 3 (kidoma virus), human polyoma virus type 4 (wu polyoma virus), astrovirus, severe acute distress syndrome-associated virus (SARS virus), eastern east syndrome virus, respiratory hepatitis virus, rotavirus, leptospira, rotavirus, rotamasa, human kania, candidum, manchurian candid, and manchurian candid's disease.
The invention relates to a capture probe group of pathogenic microorganisms, which is summarized based on detection of a large number of clinical samples of China, and is used for screening pathogenic microorganism types which are specifically captured by the capture probe group to obtain the probe group which is used for capturing 62 bacteria and 41 drug-resistant genes, 16 fungi, 14 parasites, 15 DNA viruses and 60 RNA viruses, can cover more than 95% of pathogenic microorganisms with higher detection rate in the clinical samples, and also is taken into account of the current situation that part of pathogenic microorganisms are easily covered by high-abundance human hosts in the samples and are difficult to detect, and also is taken into capture objects.
It will be appreciated that the probes may be in the form of ssDNA, dsDNA or RNA probes designed in a manner common in the art to perform a capture function.
It will be appreciated that each probe sequence in the capture probe set is specifically bound to a target region of a respective microorganism and cannot bind to any region of any other species.
In one embodiment, the length of each probe is 120+/-10 bp, and the more uniform probe length enables the combination temperature between the probes and the templates to be more uniform, so that the uniformity of the combination of each probe and the templates is improved, and the template utilization rate is increased under the condition of uniform hybridization capture temperature.
In one embodiment, the capture probe set comprises the sequences shown as SEQ ID NO. 1 through SEQ ID NO. 517. The inventor selects the capture probe group through repeated tests and screening, has better specificity and capture performance aiming at each pathogenic microorganism species, and can avoid the generation of polymers or other adverse interference influences among the probe groups.
In one embodiment, the capture probe set also specifically captures the following drug resistant genomic sequences: aac6-Ii, aac6, blaCTX-M-151, blaDHA-4, blaIMP-64, blaIMP-37, blaIMP-7, blaKPC-40, blaNDM-9, blaSHV-16, blaTEM-237, blaVIM-32, blaVIM-7, DIM, ermA, ermB, ermC, fosA3, mcr-1, mecA, mecB, mecC, mefA, msrA, nimA, nimB, OXA-23, OXA-24, OXA-48, OXA-51, qnrA9, qnrB12, qnrB85, qnrS11, SIM, sul1, sul2, tetA, vanA, vanB, vanC2, vanC.
Meanwhile, the invention also provides the drug resistance information while providing the pathogenic microorganism information by designing and adding the detection of the drug resistance genome, thereby being beneficial to the judgment of clinicians and giving reference and guidance to the medication.
In one embodiment, the capture probe set further comprises a sequence as set forth in SEQ ID NO. 518 through SEQ ID NO. 640. Similarly, the capture probe set aiming at the drug-resistant genome has the advantages of strong specificity and high capture efficiency.
In one embodiment, the detection probes are each modified with biotin. After biotin modification, the probe sequence enriched with microorganisms in the sample is captured by using streptavidin magnetic beads, then the captured sample is eluted at high temperature by using rinsing liquid, and after non-specific capture sequences are removed, PCR specific amplification is carried out, and then machine sequencing is carried out, so that the aim of rapidly and specifically enriching and detecting pathogenic microorganisms in the sample is fulfilled.
It will be appreciated that the probes described above may be biotin modified or otherwise modified, provided that capture enrichment is specifically captured by streptavidin or other corresponding components, and that the modification is performed in a conventional manner.
The invention also discloses application of the capture probe set in preparation of reagents and/or equipment for detecting pathogenic microorganisms.
The invention also discloses a kit for detecting pathogenic microorganisms, which comprises the capture probe set.
The kit comprises the capture probe set, and the detection is carried out after the target capture of pathogenic microorganisms to the mNSS library by adopting the kit, so that the detection sensitivity and the specificity of the pathogenic microorganisms can be improved, and the false negative result can be reduced.
In one embodiment, the kit further comprises a positive quality control, a negative quality control, a library construction reagent.
It will be appreciated that the positive quality control, negative quality control, library construction reagents described above may be formulated as desired by conventional methods.
The invention also discloses a detection library of pathogenic microorganisms, which is constructed by adopting the kit.
The invention also discloses a method for detecting pathogenic microorganisms for non-diagnostic purposes, which comprises the following steps:
library preparation prior to capture: taking a sample to be tested, extracting nucleic acid in the sample, and constructing a library;
liquid phase hybridization capture: taking the library obtained by the construction, adding the capture probe set, and performing hybridization capture;
PCR amplification and purification: performing PCR amplification and purification on the hybridization capture product to obtain a capture library;
high throughput sequencing: the capture library was subjected to high throughput sequencing and data analysis.
In one embodiment, the library is constructed according to 300-600bp inserts in the pre-capture library preparation step.
It will be appreciated that the disruption method of library construction may be selected from transposase disruption, enzymatic disruption, and mechanical disruption. Library construction is not limited by the sequencing platform, including but not limited to Illumina, MGI, ion-torret, etc. The key point of the invention is to provide a method for capturing and enriching specific pathogenic microorganisms, which is applicable to different changes of the pre-steps such as sample pretreatment, library preparation and the like before liquid phase hybridization capture, and adjustment of the subsequent amplification, purification and sequencing steps according to specific applicable conditions.
In one embodiment, the liquid phase hybridization capturing step is performed according to the following steps:
1) Library mixture preparation: preparing library mixed solution, and concentrating to dryness by using a vacuum concentrator for later use;
2) Hybridization: mixing the library with a capture probe set to obtain a hybridization mixed solution, carrying out denaturation at 95 ℃ for 5min on a PCR instrument, and hybridizing the probe and the nucleic acid of the sample to be detected for 1-4h according to the hybridization temperature of 60-65 ℃;
3) Capturing: the hybridization product was captured with streptavidin magnetic beads.
In one embodiment, the library mixture is configured to contain 8-16 libraries, with a total library input ranging from 1-4 μg. The library mixture also contains a library sealing solution corresponding to each library and a joint sealing solution.
In the scheme, 8-16 libraries can be hybridized and captured at one time, each library is input according to equal mass, the input initial quantity is higher than 100ng, and the situations of capture failure and low output caused by sample input quantity in hybridization and capturing are reduced as much as possible.
It will be appreciated that in practice, hybridization capture may be performed on a single library or may be performed by adding a library in an amount corresponding to the desired amount, and hybridization capture may be performed.
Preferably, the library mixture preparation method comprises the following steps: each library was dosed in an amount of 125-500ng, with 5. Mu.l of library block solution Human cot-1DNA and 200. Mu.M 2. Mu.l of linker block solution. Mixing uniformly, concentrating to dry powder in a vacuum concentrator for later use.
Preferably, the hybridization mixture includes: RNase enzyme inhibitor 5. Mu.l, hybridization buffer and capture probe set. Each probe in the capture probe set had a working concentration of 0.5pM to 5.0pM.
In one embodiment, the hybridization reaction procedure is as follows:
TABLE 1 hybridization reaction procedure
It will be appreciated that the temperature control of the hybridization cycle described above, if necessary to increase the coverage of the AT rich region of the library, may be suitably reduced, e.g., from 65℃to 60℃and adjusted to the specific hybridization requirements.
In one embodiment, when capturing the hybridization product with streptavidin magnetic beads, the capture is performed at a final concentration of 2mg/ml using streptavidin magnetic beads.
In one embodiment, the PCR amplification and purification steps are performed as follows: the captured product was enriched by PCR amplification and the amplified product was purified with DNA purification magnetic beads.
Specifically, the capture product PCR enrichment was performed using high fidelity DNA polymerase. Preferably, amplification can be performed in a 50. Mu.l system using KAPA HiFi HotStart ReadyMix. The amplification cycle number is 7-14, and the reaction system and the procedure are as follows:
TABLE 2 amplification reaction System
| Reaction system | Single dose (mu L) |
| Capturing the magnetic bead capturing mixed solution | 22.5 |
| PCR primer mixture | 2.5 |
| PCR polymerase | 25 |
TABLE 3 amplification reaction procedure
Preferably, the amplified product is purified using Beckmann Ampure XP magnetic beads. The specific method comprises the following steps: adding 25 μl ddH to the original product 2 O, 1.2× (60 μl) magnetic beads were added and purified.
In one embodiment, the sample types to be tested are: blood, alveolar lavage, sputum nasopharyngeal swab, tissue, cerebrospinal fluid or chest fluid.
It will be appreciated that the detection method of the present invention is applicable to mNGS detection of a general clinical sample. Including, but not limited to, blood, alveolar lavage, sputum nasopharyngeal swab, tissue, cerebrospinal fluid, chest water, and the like. However, the kit is particularly excellent in effect when applied to a blood sample for detecting whole blood.
Compared with the prior art, the invention has the following beneficial effects:
the invention relates to a capture probe group of pathogenic microorganisms, which is designed for 167 pathogenic microorganisms commonly detected clinically, and establishes a specific probe library, wherein the pathogen library comprises 62 bacteria, 41 drug resistance genes, 16 fungi, 14 parasites, 15 DNA viruses and 60 RNA viruses, and covers the pathogenic microorganisms detected by clinical samples to be more than 95 percent at most.
The capture probe group is used for capturing and enriching pathogenic microorganisms, so that the defects of high human host occupation ratio and low microorganism content in the conventional technology of mNGS detection, which cause the sequencing data to be mostly human, are overcome, the real pathogens are captured and enriched from a large number of human sequences, the detection sensitivity is improved, the number and the proportion of the detected sequences of the pathogenic microorganisms are greatly improved compared with a macrogenomics detection method, the median of the enrichment ratio is 300, and the detection signals of the pathogenic microorganisms are efficiently amplified.
The detection limit of fungi, bacteria and viruses is obviously reduced and the clinical positive coincidence rate of mNSS detection is improved while the sequence signals of pathogenic microorganisms are amplified.
And on the premise of not influencing the experimental detection efficiency, the experimental sequencing cost is reduced from 20M/sample of the metagenome to 1M/sample.
Drawings
FIG. 1 is a schematic diagram showing the comparison of detection limits of pathogenic microorganisms in a simulated mixed sample according to example 3;
FIG. 2 is a median plot of the enrichment efficiency of each pathogenic microorganism in a simulated mixed sample of example 3.
Detailed Description
In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The reagents used in the examples below, unless otherwise specified, are all commercially available.
Example 1
A kit for detecting pathogenic microorganisms for rapid hybridization capture of mNSS is designed by the following method.
1. Design and synthesis of capture probe sets.
Reviewing the past clinical samples, screening pathogenic microorganisms which occur at high frequency clinically, covering bacteria, fungi, DNA viruses, RNA viruses, parasites and common drug resistance genes, selecting a coding region of the pathogenic microorganisms as a target region, analyzing and screening a specific region of each species according to a known genome sequence, designing and generating a probe group in a tile array mode, wherein the probe length is about 120bp, and synthesizing a probe with biotin-labeled nucleotide.
The design of the probe requires high specificity and is not affected by homologous sequences of other closely related species. And the sequences are not affected by each other. These sequences are located in the coding region of the microorganism and are highly conserved.
The invention provides the most efficient and specific capture probe group related pathogenic microorganisms and drug resistance genes as shown below.
TABLE 4 pathogenic microorganisms and corresponding probe sequences
TABLE 5 drug resistance genes and corresponding probe sequences
2. The capture kit is used for detecting pathogenic microorganisms.
Taking the capture probe set as a core, constructing a kit, and specifically comprising the following steps:
TABLE 6 kit composition of pathogenic microorganisms
| Component (A) | Quantity of | Sources/compositions | Storage temperature |
| Capture probes | 1 pipe | Tables 4-5 design of synthetic Capture Probe set | -20℃ |
| Cationic property control product | 1 pipe | Enterprise reference | -20℃ |
| Negative quality control product | 1 pipe | HELA cell line DNA | -20℃ |
| Streptavidin magnetic bead | 1 pipe | Streptomycin magnetic bead 5mg/ml | 4-10℃ |
| Magnetic bead combination bufferFlushing liquid | 1 pipe | Tris-HCl、EDTA、NaOH、Tween-20 | 4-10℃ |
| Washing buffer 1 | 1 pipe | SSC,SDS | 4-10℃ |
| Washing buffer 2 | 1 pipe | SSC,SDS | 4-10℃ |
| Hybridization buffer | 1 pipe | SDS, SSPE, SSC, dehart solution | -20℃ |
| DNA purification magnetic bead | 1 pipe | Hydroxyl magnetic bead | 4-10℃ |
| Library blocking solution | 1 pipe | Tris-EDTA，Human cot-1 | -20℃ |
| Joint sealing liquid | 1 pipe | Oligonucleotides, TE | -20℃ |
| Amplification mix primer | 1 pipe | Oligonucleotides, TE | -20℃ |
| Amplification polymerase | 1 pipe | DNA polymerase, mgCl2, dNTPs | -20℃ |
| RNase enzyme inhibitor | 1 pipe | Rnase enzyme inhibitor, tris-EDTA | -20℃ |
Note that: the above reagent components are all conventional reagents except for the capture probe.
Example 2
A method for detecting pathogenic microorganisms using the kit of example 1, comprising the steps of:
1. library preparation prior to capture.
Extracting nucleic acid of a sample to be detected, constructing a library, and quantitatively detecting the concentration of the library after purification, wherein the final obtained library fragment is 300-600 bp.
2. And (5) liquid phase hybridization and capturing.
2.1 preparation of library mixtures:
taking 8-16 linker libraries, weighing 125ng of each library, mixing, adding concentrated reagent according to the following table, wherein corresponding linker sealing liquid is added according to Illumina or Huada library building flow, and selecting according to a subsequent sequencing platform.
TABLE 7 composition of library mixtures
| Reaction system | Amount (. Mu.L)/library |
| Library to be hybridized | x |
| Joint sealing liquid | 2 |
| Library blocking solution | 5 |
The above mixture was concentrated to a dry powder state in a cryoconcentrator at 200rpm and 60 ℃.
2.2 hybridization:
a. hybridization mixtures were prepared as shown in the following table.
TABLE 8 preparation of hybridization mixture
| Reagent(s) | Amount (. Mu.L)/library |
| Hybridization buffer | 6 |
| Capture probe set | 4 |
| RNA-inhibiting enzymes | 5 |
| Nuclease-free water | 17 |
b. Adding the mixture into the concentrated dried library, incubating for 5min at room temperature, transferring the dried powder into a PCR tube after the dried powder is fully dissolved, and hybridizing according to the following procedure:
TABLE 9 hybridization reaction procedure
And (3) a thermal cover: hybridization was performed on a PCR apparatus at 105 ℃.
2.3 capturing:
1) The preparation of the magnetic beads was performed immediately after the hybridization was started.
a. The streptavidin beads were vortexed vigorously to allow uniform resuspension of the beads.
b. 50 μl of resuspended magnetic beads were added to the new EP tube
c. 200. Mu.l of magnetic bead binding buffer was added.
b. After 20 blows, the nozzle is covered, swirled at high speed for 5-10 seconds, and then instantaneously detached.
c. The tube is placed on a magnetic rack.
d. Wait 5 minutes or until the solution becomes clear, then remove the supernatant.
Steps a to d were repeated twice for a total of 3 washes.
e. The beads were resuspended in 200. Mu.l of magnetic bead binding buffer.
2) The hybridization library was captured using streptavidin magnetic beads.
a. After the hybridization step is completed, the sample is transferred to room temperature;
b. the hybridization system (about 50. Mu.l) was immediately transferred to 200. Mu.l (wash streptavidin) magnetic bead tubes. Blowing up and down for 5-8 times.
c. The tube was placed on a mixer and reacted at room temperature for 30 minutes.
3) During the capture incubation period of 30 minutes, wash buffer 2 was pre-warmed at 70 ℃.
a. 930 μl of wash buffer 2 was added to the new EP tube for each reaction and incubated in a 70℃metal bath until use.
4) After the mixing by the mixing instrument is finished for 30min, the liquid drops on the pipe wall are removed by instantaneous separation.
5) The tube is placed on a magnetic rack. After the solution was clear, the supernatant was discarded.
6) The beads were washed using wash buffer 1 as follows.
a. 200 μl wash buffer 1 was added at room temperature for resuspension. The beads were blown up and down 15-20 times until the beads were completely resuspended.
b. The resuspended beads were incubated for 5min at room temperature.
c. The tube was transferred to a magnet rack at room temperature. After the solution was clear, the supernatant was discarded.
d. Repeating a, through b. The incubated bead mix was transferred to a new EP tube and the tube was transferred to a magnetic rack at room temperature. After the solution was clear, the supernatant was discarded.
7) The beads were washed using wash buffer 2 as follows.
a. 200 μl wash buffer 2 was added at 70deg.C for resuspension. The beads were blown up and down 15-20 times until the beads were completely resuspended.
b. The resuspended beads were incubated on a metal bath at 70℃for 5min.
c. The tube was transferred to a magnet rack at room temperature. After the solution was clear, the supernatant was discarded.
d. Repeat a, through c, twice for a total of 3 washes.
8) After confirming Buffer air-drying in the tube, 25. Mu.l of nuclease water was added to each sample well. The beads were resuspended by blowing up and down 20 times. The sample was placed on ice for further use.
3. PCR amplification and purification
3.1PCR amplification
The PCR reaction system was prepared according to the amplification system shown in Table 2 of the present invention, followed by 9 amplification cycles according to the amplification reaction program of Table 3.
3.2 purification
1) Purification step
The DNA purification magnetic beads are equilibrated for 30min at room temperature in advance, and are uniformly mixed by vortex before use.
b. 90. Mu.L (1.8X) of the resuspended magnetic beads were added to the reaction system obtained in the previous step.
c. Vortex mixing, instantaneous separation, and incubating for 5min at room temperature.
d. The tube was transferred to a magnet rack at room temperature. After the solution was clear, the supernatant was discarded.
e. The tube was charged with 300 μl of freshly prepared 80% ethanol on a magnet rack.
f. The tube was rotated for two weeks and the supernatant discarded.
g. Repeating e.to f. Residual ethanol was removed by suction with a small gun head.
h. Drying the metal bath at 37 ℃ until the surface of the magnetic beads is matt.
i. 30. Mu.L of de-ribotide water was added.
j. Vortex mixing, instantaneous separation, and incubating for 5min at room temperature.
k. The tube was transferred to a magnetic rack and the supernatant was collected, i.e. the library was captured, after the solution was clarified.
2) Qubit determines library concentration.
3) Library fragments were detected with Aglient 2100.
4. High throughput sequencing.
1) The on-press library was prepared according to the instructions of Illumina sequencing kit and high throughput sequencing was run on the Miniseq platform. The machine was started up at 1M/sample.
2) And analyzing sequencing data of the captured library by using a biological analysis flow to obtain a pathogenic microorganism detection result.
Example 3
And (3) comparing the conventional metagenome detection with the detection limit of the probe hybridization capture method.
1. The method.
1.1A simulated mixed sample was prepared.
The simulated mixed samples contained human HELA cells, acinetobacter baumannii (Acinetobacter baumannii, baumann), klebsiella pneumoniae (Klebsiella pneumoniae, pneumogram), streptococcus agalactiae (Streptococcus agalactiae, GBS) DNA, streptococcus pyogenes (Streptococcus pyogenes, suppuration), cryptococcus neoformans (Cryptococcus neoformans), candida albicans (Candida albicans), human herpesvirus type i (HSV 1), human herpesvirus type 4 (EBV).
Each mock mix sample contained HELA cells 2X 10 5 The prepared pathogenic microorganism mixed solution was subjected to 3X gradient dilution with sterile PBS per ml, and the same amount of the diluted solution was added to HELA cells, and the concentration of pathogenic microorganisms was finally obtained as shown in the following table.
TABLE 10 simulation of pathogenic microorganisms and concentrations in mixed samples
1.2 library preparation:
1) Taking 500 mu l of each pathogen gradient simulation sample, carrying out wall breaking treatment by using a homogenizer, sucking 300 mu l of each pathogen gradient simulation sample, and extracting and purifying DNA by using a nucleic acid extraction or purification reagent.
2) The library construction was performed using a genomic DNA fragmentation kit (transposase method), taking care to avoid linker duplication, to obtain a conventional library.
1.3 hybridization Capture and purification:
hybridization capture and purification were performed as in example 2 to obtain a capture library.
1.4 on-machine sequencing:
NGS sequencing was run on Illumina Nextseq 550 platform separately for the conventional library and the corresponding capture library, with mNGS library sequenced per 20M requirement and capture library sequenced per 1M requirement.
2. As a result.
2.1 the sequencing data amount described above was analyzed and counted.
TABLE 11 sequencing data volume
| Numbering device | mNGS | Capturing |
| Y1 | 22,381,480 | 993,462 |
| Y2 | 15,595,546 | 900,154 |
| Y3 | 16,583,424 | 940,658 |
| Y4 | 16,228,006 | 854,561 |
| Y5 | 14,773,402 | 832,387 |
| Y6 | 19,241,448 | 735,396 |
| Y7 | 17077426 | 501053 |
2.2 simulation of the detection results of pathogenic microorganisms in the mixed sample.
And carrying out a letter flow analysis on the captured library, and counting the sequencing depth, the number of detected sequences and the enrichment efficiency of the sample. The results obtained are shown in the following table.
TABLE 12 simulation of the detection results of pathogenic microorganisms in the mixed samples
Note that: "-" indicates undetected, "+" indicates yin-to-yang.
FIG. 1 is a schematic diagram of analysis comparison of detection limits, and FIG. 2 is a median diagram of enrichment efficiency of a simulation sample. As can be seen from the above table and FIGS. 1-2, the capture probe set for pathogenic microorganisms of the present invention improves the sensitivity of mNSS detection and significantly amplifies the detection signal of pathogenic microorganisms. Under the same conditions as conventional mNGS, the hybridization capture scheme of the invention does not have the phenomena of undetected and undetected.
Meanwhile, the amplification efficiency of the detection signal is remarkable when the required data volume is reduced to 20-40 times, which proves that the sequencing cost can be saved.
Example 4
The whole blood/plasma simulated sample routine metagenome detection and the probe hybridization capture method of the invention detect the contrast experiment.
1. The method.
1.1A simulated mixed sample was prepared.
The simulated mixed samples contained healthy human whole blood, acinetobacter baumannii (Acinetobacter baumannii), streptococcus pneumoniae (Streptococcus pneumoniae), staphylococcus aureus (Staphylococcus aureus), enterococcus faecium (Enterococcus faecium), candida albicans (Candida albicans) and human herpesvirus type 5 (CMV). Diluting the pathogenic mixed solution with whole blood to corresponding concentration to obtain the final concentration of pathogenic microorganism as shown in the following table,
TABLE 13 simulation of pathogenic microorganisms and concentration (cfu/ml) in mixed samples
| Input amount | Candida albicans | Streptococcus pneumoniae | Staphylococcus aureus | CMV | Acinetobacter baumannii | Enterococcus faecium |
| Y1 | 1000 | 500 | 1000 | 50000 | 1000 | 500 |
| Y2 | 333 | 167 | 333 | 16666 | 333 | 167 |
| Y3 | 111 | 55 | 111 | 5555 | 111 | 55 |
| Y4 | 33 | 18 | 33 | 1851 | 37 | 18 |
| Y5 | 11 | 6 | 11 | 617 | 12 | 6 |
| Y6 | 4 | 2 | 4 | 205 | 4 | 2 |
| Y7 | 1 | 0 | 1 | 68 | 1 | 1 |
1.2 nucleic acid extraction
1ml of whole blood is taken for 1600g centrifugation for 10min, 500 mu L of whole blood is taken for extraction after plasma separation, and 500 mu L of whole blood is taken for extraction for each sample, namely, the whole blood sample and the plasma sample are respectively represented.
2. As a result.
The conventional library (hereinafter "mNGS") and the corresponding hybrid capture library (hereinafter "capture") were each run NGS sequencing on Illumina Nextseq 550 platform, followed by bioinformatic flow analysis, counting the sequencing depth, number of sequences detected, and enrichment efficiency of the samples. The results obtained are shown in the following table.
TABLE 14 simulation of the detection results of pathogenic microorganisms in the mixed samples
Note that: "-" indicates undetected, "+" indicates yin-to-yang.
From the above table, it can be seen that in the detection of the simulated sample, the whole blood capture procedure can reach a lower pathogen detection limit than plasma mNGS, whole blood mNGS and plasma capture, indicating that it reaches a better detection sensitivity.
The capture probe group of the invention is used for detection, which not only improves the phenomenon of weak pathogenic signals caused by high host rate of whole blood mNGS, but also avoids the phenomenon of losing and missing detection of pathogenic microorganisms which are mainly bacteria and fungi and possibly caused by taking blood plasma as a nucleic acid extraction object.
Example 5
Clinical whole blood sample capture performance detection experiments.
8 clinical blood samples were taken and pooled and hybridized captured by centrifugation and plasma extraction, respectively, with reference to whole blood from example 4. Specific operational procedure was as in example 2.
Sequencing data were analyzed according to the signaling procedure to obtain the following results.
TABLE 15 detection results of pathogenic microorganisms in clinical blood samples
From the above table, both plasma and whole blood mNGS can cause loss of part of the pathogen. At the same time, the number of positive results captured for the plasma samples is also lower than the whole blood sample. Thus, the detection sensitivity of the whole blood sample after hybridization capture by the capture probe set of the invention is obviously higher than that of the whole blood mNGS, the whole blood mNGS and the plasma capture method.
Example 6
And (3) performing hybridization capture detection experiments on cerebrospinal fluid samples.
5 samples of cerebrospinal fluid with a high clinical suspicion of infection were randomly taken, extracted, pooled and captured by hybridization following the same procedure as in example 2. And carrying out a letter flow analysis on the captured library, and counting the sequencing depth, the number of detected sequences and the enrichment efficiency of the sample. The results obtained are shown in the following table.
TABLE 16 detection results of pathogenic microorganisms in clinical cerebrospinal fluid samples
The invention has better enrichment effect on the detection of the cerebrospinal fluid sample, and obviously improves the detection sensitivity of the cerebrospinal fluid sample.
Example 7
And (3) testing the capturing performance of the respiratory tract sample and analyzing the drug resistance gene.
5 cases of alveolar lavage fluid with clinical high suspicion of infection were randomly taken, extracted, pooled and hybridization captured according to the same procedure as in example 2.
According to the raw message flow analysis, the following results are obtained:
TABLE 17 detection results of pathogenic microorganisms in clinical respiratory tract samples
The drug resistance genes were also analyzed, and the results are shown in the following table.
TABLE 18 detection results of pathogenic microorganisms in clinical respiratory tract samples
From the above results, the hybridization capture in the respiratory tract sample is better in capture performance and higher in sensitivity. And the detection sensitivity of pathogenic microorganisms is improved, so that the detection of drug-resistant genes is more sensitive, and the coverage is improved.
Example 8
Alveolar lavage samples were taken at random for 6 cases of clinical highly suspected infection, but with negative results from conventional mNGS testing. Extraction, pooling and hybridization capture were performed according to the same procedure as in example 2.
Analysis was performed according to the letter flow, and the following results were obtained.
Table 19. Results of rechecking of mNGS negative samples
From the above results, it is clear that clinical sample detection has higher sensitivity than conventional macrogenomics by liquid phase capture technique.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
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<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 20
aatcatttaa acaaagggtt tgtctttaat gattgcagac aacgcctagt tgtttggtgg 60
gaggagtgct taatgcacca ggattgggtg gaaccagcaa agtgtatctt gggtggaact 120
<210> 21
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 21
atcatattct gaattaataa aaagtggaaa aaatccttac ttatatttca ataaagctga 60
tgaaaaattc attgacgatt tgaaaaacga ctggtctctt ggtggcatta ttggctcaag 120
<210> 22
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 22
ttccactaac catgacatct actctgtagt tggtggcaat actgtttctc atgttcatgc 60
agcaccatta aaagaacgag ttcttcagct aaattttatg aaacaactac cacaaacatt 120
<210> 23
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 23
actaggcttg ctagaaatgg cactaataat atttttaatg aaatgaaaca aaaatttcaa 60
gaactacaaa tagatgggaa aatcagttgg gatactacta gagaactatt gtttactcag 120
<210> 24
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 24
gatacttggc aatcacaacc tagagacgac gatgagctca gaatctacga agaacaggca 60
tcgcagttcc aaaagaacac caagtccact tcagaaagag aggaagcgca actgggagaa 120
<210> 25
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 25
gaagaattca cgatggttgg aagaagagca acagccatcc taaggaaagc aactagaagg 60
ctgattcagt tgatagtaag tggaagagat gaacaatcaa ttgctgaagc aattattgta 120
<210> 26
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 26
ggttcattga aattggagtg acacgaaggg aagttcatat atactatcta gagaaagcca 60
acaagataaa atccgagaag acacacatcc acatattctc attcaccgga gaagaaatgg 120
<210> 27
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 27
atgtggtaaa ctttgtgagt atggaattct ctcttactga tccgagactg gagccacaca 60
agtgggaaaa gtactgtgtc ctcgaaatag gagacatgct cctacggact gcaataggcc 120
<210> 28
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 28
tgatggtatc acctctggca gtaacatggt ggaataggaa tggtccaaca acaagtacag 60
ttcattaccc taaggtatat aaaacttatt tcgaaaaggt cgaaaggttg aaacatggta 120
<210> 29
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 29
ccagaattgt aaaattgctc ccttgatggt ggcgtacatg ctagaaagag aattggtccg 60
taaaacaagg tttctcccgg tagccggtgg gacaagcagt gtttatattg aggtgttgca 120
<210> 30
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 30
catctctctt ggaaatgtgc cacagcacac agattggagg tgtgaagatg gtggacatcc 60
ttaaacagaa tccaactgag gagcaagccg tagacatatg caaggcagca atagggttga 120
<210> 31
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 31
agagcaatga tggaccaagt gcgggagagc cgcaaccctg ggaatgctga gattgaggat 60
ctcatcttcc tggcaagatc agcactcatc ctgagggggt cagtagccca caagtcatgc 120
<210> 32
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 32
tgctaaaagg tcatacaaca acacaagtgg ggagcaaatg ctgataatct ggggggtaca 60
tcatcccaat gacgatgcag aacaaaggac actgtaccag aatgtgggaa catatgtttc 120
<210> 33
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 33
acaaccaagt ggtgccatgt gagccgatca taatagaaaa gaacgtaaca gaaatagtgt 60
acctaaataa taccatcatc gaaagggaga tttgtcccaa atcagcagaa tacagaaatt 120
<210> 34
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 34
cctggaaatg acaatagcac ggcaacgctg tgccttgggc accatgcagt accaaacgga 60
acgatagtga aaacaatcac aaatgaccga attgaagtta ctaatgctac tgagttggtt 120
<210> 35
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 35
ctattagggg accctcagtg tgacggcttt caaaataaga aatgggacct ttttgttgaa 60
cgaagcagag cctacagcaa ctgttaccct tatgatgtgc cggattatgc ctcccttagg 120
<210> 36
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 36
gcttgcataa ggggatctag tagtagtttc tttagtagat taaattggtt gacccactta 60
aactacacat atccagcact gaacgtgact atgccaaaca aggaacaatt tgacaaattg 120
<210> 37
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 37
tgatggatgc ccttaaatta agcatcgagg acccgagcca tgaaggggaa ggcataccgc 60
tatatgatgc aataaaatgc atgaagacat ttttcggctg gaaagagccc aacattgtaa 120
<210> 38
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 38
gggggctata tgaagcaatt gaggagtgcc tgattaacga tccctgggtt ttgcttaatg 60
cgtcctggtt caactccttc ctcacacatg cactaaaata gttgtggcaa tgctactatt 120
<210> 39
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 39
atctcaatat gggccagtca gtcatttcaa acaaggttcc aacaccaaac tgaaccaatc 60
agaaatacta actttcttac tgaaaacgct gtggcttcag taacattggc gggcaactcg 120
<210> 40
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 40
gaagtcctac ataaacagga caggaacatt tgaattcaca agttttttct accgctatgg 60
atttgtagcc aacttcagca tggagttgcc cagctttgga gtgtctggga tcaatgaatc 120
<210> 41
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 41
aacagaccaa actctatggg agtggaaaca aattgataac agtaggaagc tcgaaatacc 60
agcagtcatt caccccaagt ccgggggcac gaccacaggt gaatgggcaa tcgggaagga 120
<210> 42
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 42
aaataacaaa ggagaagaaa gaagaactcc aggattgcaa gatcgcccct ctgatggttg 60
catacatgct agaaagagag ttggtccgca aaacaaggtt tctcccagtg gctggtggaa 120
<210> 43
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 43
ggggagttca tcactctgaa tcggttagcg agcagaccaa actctatgga agtgaaaaca 60
agttgataaa agtaagaagc tcaaaatacc aacaatcatt taccccaaat cctggagcac 120
<210> 44
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 44
tgataataac aattggtctg gttattctgg tattttctct gttgaacaca aaagctgtat 60
caataggtgt ttttatgtgg agttaataag aggaaggccg aaagaaacta gagtatggtg 120
<210> 45
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 45
agcagcacac ttgggctcga cctgcgagtg gccacaatgg aaggcaaaaa ggttgttgaa 60
gaaatcctaa agaatgaaac tgatgaaaat ctcagaattg caattgcatc cagccctgca 120
<210> 46
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 46
catgcacgac cattaatcaa aacaacacta ctgttgtgga aaacacatac gtaaataata 60
caacaataat taccaaggga gctgaatcga aaacatcaag ctatctgctg ctgaacaaga 120
<210> 47
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 47
gggttccagc attcaaatga ccaaggggtt ggtatggcag cagatcgtga ctcaacccaa 60
aaggcaattg ataaaataac atccaaagtg aataacatag tcgacaaaat gaacaagcag 120
<210> 48
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 48
gtcttaagga tgatcttatt gaaaatttgc aggcctacca gaaccggatg ggagtgcaac 60
tgcagcggtt caagtgaccc actcggtgtt gcagctaacg ttattgggat attgcacctg 120
<210> 49
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 49
gatagctcca gcagcagcaa ctgcagagac cctaacaacg aaagaggggc cccaggagtg 60
aaagggtggg cttttgacga cgggaatgat gtttggatgg gacggacaat caaaaatggt 120
<210> 50
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 50
aaatccatat tttcttttca tagatgtacc tatacaggca gcaatttcaa caacattccc 60
atacaccggt gttccccctt attctcatgg aacgggaaca ggctacacaa tagacaccgt 120
<210> 51
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 51
tagaacacac gagtactcaa acaagggaaa acaatacatt tctgatgtta caggatgtgt 60
aatggtagat ccaacaaatg ggccattacc cgaagacaat gaaccgagtg cctatgcaca 120
<210> 52
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 52
cctgctgcaa cggcactgaa cacaacaata acctctttta ggttgaatga tttaaatgga 60
gccgacaagg gtggattagt gcccttttgc caagatatca ttgattcatt agacaaacct 120
<210> 53
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 53
ttattttgga ttgaagccaa tatgataatg agggggtgga gtttgtgacg tggcgcgggg 60
cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt gtgatgttgt aagtgtggcg 120
<210> 54
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 54
ccggatgtgg taaaagtgac gtttttggtg tgcgccggtg tacacgggaa gtgacaattt 60
tcgcgcggtt ttaggcggat gttgtagtaa atttgggcgt aaccaagtaa aatttggcca 120
<210> 55
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 55
atgagacata ttatctgcca cggaggtgtt attaccgaag aaatggccgc cagtcttttg 60
gaccagctga tcgaagaggt actgactgat aatcttccac ctcctagcca ttttgaacca 120
<210> 56
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 56
agtctaaggg tttcgtgttc cgtcacgaga ttccattcta caaacgcctt acgcgaggtt 60
ctgtctcgtg tttgtgtgga agcaaagttt tgtctttgtg gaaaccagta actgttccta 120
<210> 57
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 57
cctgcaaccg tgtgacactt gccgtagcaa gtgattctga aatttctgca tatggctgtt 60
ctactattgc gcaagccgtc cgccgttata gcgaggccgc tagcaatggt tttagggcat 120
<210> 58
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 58
atttgcagga ttgcatcgtt ggcattgcag atgatacata tgttatgggt ctgcatggca 60
atcagacgtt gttttgcaac ataatgaaat tttctgaccg tccttttatg cttcatgggt 120
<210> 59
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 59
gtgacgtgtt ggttgtcctc agcgtccctc ccataggtcg caatgattaa aaccagcaaa 60
tacggtctcg gcttcaagtg ggcgccagaa tttcgttggc tgcttccgga tgcagcggag 120
<210> 60
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 60
gagttggcta gtcctatgaa gtcagatgag ggtgggttat gcccctctac tggtcaagcg 60
atggaaagtg ttggattcgt ttatgataat catgtgaaga tagattgtcg ctgcattctt 120
<210> 61
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 61
tttaattaaa tcacctttgc atagcttggg tggttttcct aaagggtatg ttatgggctt 60
gttccgttca tacaagacta aacgttatgt tgtacatcat ctttctatga ctacatctac 120
<210> 62
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 62
ttttctgtgt ctaagcactg gtgattctgt tcactagtgc atacactgat atttaagtgg 60
tgttccgtca ctgcttattg tggaagcaac gttctgtcgt tgtggaaacc aataactgct 120
<210> 63
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 63
aaaacatgtg ggaatttagg gattactttg ataataatac tgatagtatt gttattgatg 60
gtgtcactta tcaactagca tgggatgtta tacgtaaaga cctttcttat gaacagcaaa 120
<210> 64
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 64
atgttttagc cattgagagc attcattacc ttggtactac aggtcatact ttgaagtccg 60
gttgcaaact tattaatgcc aaaccgccta aatattcttc taaggttgtt ttgagtggtg 120
<210> 65
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 65
ctttcagcca gggacgtgtt gtatcctagg cagtggccct cccataggtc acaatgtcga 60
agatcaacaa atacggtctc gaactacact gggctccaga atttccatgg atgtttgagg 120
<210> 66
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 66
aggagaagtt ggacaaccct agtagttcag aggtggatat gatttgctcc accactgcgc 60
aaaagctgga aacagacgga atttgtcctg aaaatcatgt gatggtggat tgtcgccgac 120
<210> 67
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 67
agtcccgtga agcagttttg gttacaaccc ccttaggtat gtccttagag gcatgctatg 60
tgagaggttg taatcctaaa ggatggacca tgggtttgtt tcggcgtaga agtgtttgta 120
<210> 68
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 68
aggaaaaact tgtttggaat atataataat attaaatagt attttagggt taaagtaata 60
ctttaaaggg acaagtcaca gacatttgat cttagtataa atttttataa tggccgggct 120
<210> 69
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 69
tccaggagaa gtgagagcat caataagtct ggcggaggag caattatacc tggtcaaaga 60
agtaccgttt ctgtcttcac attaggcccg agtgtgacag atgatgcaga taaattatta 120
<210> 70
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 70
accactttct tagcccactc attggacaca gataaacaac actctcaaag aggaggattt 60
ttagtatcac tccttgcaat ggcctacagt agtccggagt tatatctcac tacaaacggt 120
<210> 71
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 71
gtttggaaat ataaatttaa attaaaatta acttaggatt aaagacattg actagaaggt 60
caagaaaagg gaactctaca atttcaaaaa tgttgagcct atttgataca tttaatgcac 120
<210> 72
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 72
aaaacataac aaaatcagct ggtggagcta tcatccctgg acagaaaaat actgtctcta 60
tattcgctct tggaccgaca ataaccgatg ataatgagaa aatgacatta gctcttctat 120
<210> 73
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 73
tggtgtctct attgtcaatg gcttatgcca atccagaact ctacttaaca acaaatggaa 60
gtaatgcaga tgtcaagtat gtcatataca tgattgagaa agatccaaaa cggcaaaagt 120
<210> 74
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 74
ggaaccacta gattcggtgc cggtaacgat tccagtttta tactatctga tcattctcta 60
tctctattaa ggatatttct agtctaaagt tcaaaatgtc aagtgtttta aagacatttg 120
<210> 75
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 75
agagggacat agagctggtg ctctcctcag tcttttatca ctaccttctg cagctatgag 60
taatcacatc aaattagcca tgcattcacc agaagccagc atagatagag tagagataac 120
<210> 76
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 76
ataattcatt ccgagtcatt ccagatgctc gatcaactat gtccagagga gaggtgctgg 60
cttttgaagc attagctgag gacattcctg atacccttaa tcaccaaact ccatttgtaa 120
<210> 77
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 77
ttaaataaag tagctcatca ataaatcatc atgtcttcag ttttagctgc ttacgaacag 60
tttcttcaaa caactgaaga tcggggtttt ggtgatcaac aatttgtgca atctgatact 120
<210> 78
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 78
ccggcaagct gtatcatcat ctgccaaatc ggcaattaaa caaggggcat tattatctct 60
gctttcctta caggccacat caatgcagaa tcatcttatg attgctgcca gagccccaga 120
<210> 79
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 79
cattgaagtt gatgcaatag acccgccaga ttatacatta actattaatc caagatctgg 60
ctgggatgac atcaagatca gagcttatag agcattatcc agagatttgc ctattagctt 120
<210> 80
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 80
tgggcagcaa ctcattgagt atgataaaag ttagattgca aaatctgttt gacaatgatg 60
aagtagcatt gttaaaaata acatgctata ctgacaaatt aatacagtta actaatgctt 120
<210> 81
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 81
gtacatgtta ttacaagtag tgatatttgc cctaataata atattgtagt gaaatccaat 60
ttcacaacaa tgccagtatt acaaaatgga ggttatatat gggaaatgat ggaattaaca 120
<210> 82
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 82
ttctccaaaa aactaagtga ttcaacaatg accaattata tgaatcaatt atctgaatta 60
cttggatttg acctcaatcc ataaatcata ataaatatca actagcaaat caatgtcact 120
<210> 83
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 83
gggcaaataa gaatttgata agtgctattt aagtctaacc tttttaatca gaaatggggt 60
gcaattcact gagcatgata aaggttagat tacaaaattt gtttgataat gacgaagtag 120
<210> 84
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 84
ttgttaaaaa taacatgtta tactgacaaa ttaattcttc tgactaatgc attagccaag 60
gcaacaatac atacaattaa attaaacggc atagttttta tacatgttat aacaagcagt 120
<210> 85
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 85
gaagtgtgcc ctgataacaa tattgtagtg aaatctaact ttacaacaat gccaatatta 60
caaaatggag gatacatatg ggaattgatt gaattgacac actgctctca attaaatggt 120
<210> 86
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 86
ctgggagtgg gttgttccca ctcactccac ccatgcggtg ttgtactctg ttattacggt 60
aactttgtac gccagttttt cccacccttc cccataatgt aacttagaag tttgtacaat 120
<210> 87
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 87
tgacaatcat ccagactgtc aaaggtcaag cacttctgtt tccccggtca atgaggatat 60
gctttaccca aggcaaaaac cttagagatc gttatcccca cactgcctac acagagccca 120
<210> 88
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 88
ttgatataat tgggttggtc gctccctgca aacccagcag tagacctggc agatgaggct 60
ggacattccc cactggcgac agtggtccag cctgcgtggc tgcctgctca cccttcttgg 120
<210> 89
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 89
cattaaagta caataggtgg cgccatatcc aatggtgtct atgtacaagc acttctgttt 60
cccaggagcg aggtataggc tgtacccact gccaaaagcc tttaaccgtt atccgccaac 120
<210> 90
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 90
aacagttagt accatcttgt tcttgactgg acgttcgatc aggtggattt tccctccact 60
agtttggtcg atgaggctag gaattcccca cgggtgaccg tgtcctagcc tgcgtggcgg 120
<210> 91
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 91
caacccagct tatgctggga cgccctttta aggacatggt gtgaagactc gcatgtgctt 60
ggttgtgagt cctccggccc ctgaatgcgg ctaaccttaa ccctagagcc ttatgccacg 120
<210> 92
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 92
taaaactggg ctcgggttgt acccacccga gctgcccatg tggcgtggtg ctcttgtatc 60
ccggtacact tgcacgccag tttgcctctc cttaccccgt aacatatcta gaagaatgtg 120
<210> 93
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 93
ggaccaatag gtggtggcaa cccatgccac tgacggtcaa gtacttctgt ttccccggtg 60
tgatgtggaa tagactctct cagggttgaa gccataagca tcgttatccg ctacgtgcct 120
<210> 94
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 94
acctagtagc attttgaagc ttacttggtt ggccgctcag ccactaaccc gtggtaggcc 60
tggcagatga ggctgggcgc accccactgg cgacagtggc ccagcctgcg tggctgcctg 120
<210> 95
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 95
tacacaaggt gaaatagtta gtaatgagaa aagtggggtg tgtattaacg caccggcaaa 60
gactaaactt caacccagtg tcttccatca ggtttttgaa ggttcaaagg aaccagcagt 120
<210> 96
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 96
gttcccactc caagggccca tgtggcggct agtacactgg tacttcggta cctttgtacg 60
cctgttttat ctccctcccc aatgtaactt agaagctttc aaatcaaagc tcaataggtg 120
<210> 97
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 97
cactcctcca ggcgggtcat gcccgacaac cagagaaact gctatgttag gcacacatgt 60
tgtttgggat tttggattac aatccagtgt caccctagta ataccttgga ttagtggttc 120
<210> 98
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 98
gaaaatgtct cttcaaggga ttcacctgag tgatttatca tacaagcatg ctatattaaa 60
agagtctcag tacacaataa aaagagatgt gggtacaaca actgcagtga caccctcatc 120
<210> 99
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 99
acaagaaata acactgttgt gtggagaaat tctgtatgct aaacatgctg actacaaata 60
tgctgcagaa ataggaatac aatatattag cacagcttta ggatcagaga gagtgcagca 120
<210> 100
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 100
taaccagaac gtactctctg gggaaaatta aaaacaataa aggagaagat ttacagatgt 60
tagacataca cggggtagag aagagctggg tagaagagat agacaaagaa gcaaggaaaa 120
<210> 101
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 101
attgggggaa tcgtcactgc cgcccctttg gggaggggaa aggcgtgggg tataagttag 60
ccctggcccg acggtctggt cgcatttgca cctcggcact cggagcgaga cgcagcagcc 120
<210> 102
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 102
gcgccatgtt ggggcgccat gttagggggc gtggaacccc gtgacactat atatacaggg 60
accgggggcg ccatgttagg gggcgcggaa ccccctgacc ctatatatac agggaccggg 120
<210> 103
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 103
actgactcat acgcatggcc cccttactca cacgcatcta ggggggtggg gaggagccgc 60
ccgccatatt tgggggacgc cgtgggaccc ccgactccgg tgcgtctgga gggcgggaga 120
<210> 104
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 104
ggcgggagga ggagacccgg agagacagag actaaaaccc ggcaagagag agaccgcggg 60
ccgccgtctc gagtctaccc taccccggct catggaaccc cggcccggca cgagctcccg 120
<210> 105
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 105
gcgctacctg cccatcgcgg gggtctcgag cgtcgtggcc ctggcgcctt acgtgaacaa 60
gacggtcacg ggggactgcc tgcccgtcct ggacatggag acgggccaca taggggccta 120
<210> 106
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 106
cgcgcaactg cgtgaggccc cccgactacc cgacgccccc cgcgtcggag tggaacagcc 60
tctggatgac cccggtgggc aacatgctct ttgaccaggg caccctggtg ggcgcgctgg 120
<210> 107
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 107
atttttagct gcacgcacca aaaataagcg tgacaattac gtgttcccag aacaattcga 60
attttgtcat gcaaaggtgt agaaatagcg gtttttacca tagtatctcc tgataataga 120
<210> 108
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 108
gacaagtgaa cccttttgtt ccgcatatga gctgaaataa cacccagtcc cttttgatgg 60
cgatacactt tgatgatgtt aaggtatatt cgcgatcacg cccggggaaa tgaacagcaa 120
<210> 109
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 109
taagtcatat aagggggttt ccaacgtatt gtaacttgtg tccgtttgta acaagtaata 60
gcgtgtagcc aacactagcg ttttttcaga gggtccaaat cgaacaatat accaaaacgg 120
<210> 110
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 110
atcttgttta gcaattcgta aactgtcagc ctagcgacgt ccgtttagat caaaagtcac 60
gtatatagcg acgctgtttc cacccgtttc cccgtcccgc cgtttccgaa caacccaccc 120
<210> 111
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 111
cagttctacg gaggcctgat tttccacacc acctgggtca ccggcttcgt cctgttagga 60
ctcttgacgc ttttcgccag cctgtttcgc gtaccgcaat ccatctgtcg tttctgcata 120
<210> 112
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 112
gtttccaaac cgtaacgtgg taaataaatc atggcttctg acgtgggttc tcatcctctg 60
acggttacac gattccgctg cagagtgcat tatgtgtaca ataaactgtt gattttaact 120
<210> 113
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 113
cgttttgtct actacaccgg gtacattttg gtttccctca gggttcgacc ctaaacccta 60
ccatccttcg gccgacagca agttgctacc gctgggccta attacgctgt ccgcctgttc 120
<210> 114
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 114
aacagaataa aggctaaaca ccggccaaac gctcatcctt ctccgtacgt cccctttccg 60
taagtgccaa atgccacata tccatgaacg gacacacgcc ggcagaatcc ggccttcctg 120
<210> 115
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 115
ggcgggactt cgagacgagc cacccttcgt atacgtccgg cccctcccac gcgcgcctat 60
aggacgcacc ccctcgttgc gaaaccgcaa cagggcacag ttgccgtcac accccctgtc 120
<210> 116
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 116
cggcggctgt cgccgtcacg gtagtcgtgg cggagcaccc ggggaggcga cgacggggac 60
taccgatgct ctcagatcat cgccatagtg aaagagtacc gaataaaaca agttaaattt 120
<210> 117
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 117
ccgaccctgc cgcggcaacg aaggcgaaac gagaccccga cgcatgcggg cataaagagc 60
gagaagtaaa cgcggggtcc cgaattatcg cgagactatt tgcccgggcc cgccctcgtc 120
<210> 118
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 118
acggcaagat ctgccgtctg gcgaactcgt tcgccgccct gtggaggatg gggccgcggg 60
ccatgagagg acattggacg tattcggccc cgggtagaca tctccccggg gacgcttggc 120
<210> 119
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 119
aacaacgcca catgtaatgg acgcagtctg cggtggccgc gggtcatggc tgtccatcgg 60
ctacctagta aagatgccgc acattcacct ggcggtgacc cgaacatgtc tggtcaccgc 120
<210> 120
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 120
caccggtagt cttttactac tgtcgcggcc gaccgcagac ttgacgtctt ggtcatgttt 60
acagcaagaa cctgtgtgga ggaactgtct agatacgcgc ggagaacagg atgagacaga 120
<210> 121
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 121
aatacgggtt ccgctataaa aagtgcacct ctattcccgt tcttatcccc gttctaactc 60
ttccttgtat cataccttgc atgttaaccg gatcccgtgg atcttacaca catacacaca 120
<210> 122
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 122
atcgtgtccg cgctgcgaga cacttactcg ggccacttgg tccttcagcc cacggagacc 60
cttgacacat ggaaggtgtt gagcagggac acacgaaccg ctcatagttt ggagcacgga 120
<210> 123
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 123
tctcccttct tcaccgtgga gtttcccccg attcaagagg agggcgcaag ttctccggta 60
cccttagatg tggacgagag catggacatc tctccgtctt acgagttgcc ctggctctcg 120
<210> 124
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 124
cggtggggct cctcccttgc cctatcgtac ctgggatcga atgacagaga agctgcttgt 60
ctccgcaaaa cccggcggag agaacgttaa ggtttcaggt accgtgatta cattgggaga 120
<210> 125
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 125
cacgggtttt ttgttgtctt ggccaatcgt gtctccatgg cgctaaaggg accacaaacc 60
ctcgaggaaa atattgggtc tgcggccccc actggtccct gcgggtacct ctatgcctat 120
<210> 126
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 126
agcaacaaca caaagggacg ctctggagcc cagaggcatg ccctcagcta ccctgcgcga 60
atgagatttt tatggcggtc atagttacag agggattcaa ggagagactg tacggcggca 120
<210> 127
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 127
atgaaggaca ggttcccacc tgttctgtca aacgttaaga gagacccata tgtgatcacg 60
ggcacagcgg gaacgtacaa tgacctagag attctcggaa actttgccac cttcagggag 120
<210> 128
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 128
gcttgcctag ggccttataa cccgacttgc tgagcttcta taggaaaaaa ccctttccca 60
gccttggggt ggctggtcaa taaaaacccc catagtaacc aacacctaag acaatttgat 120
<210> 129
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 129
tgtaaatgat cttttctaac ctgtattatg tacagggtgg cagatggcgt gccataaatc 60
tattagtggg ataccacgct tgtggacctt atgcccacac agccatcctc tagtaagttt 120
<210> 130
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 130
gtaaaatgtc tggtgagatg tgggaactta ttggaaacaa caatttgctt aatagcatcc 60
tagtgccagc ggaacaacat ctggtaacag atgcctctgg ggccaaaagc caaggtttga 120
<210> 131
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 131
ataagccaaa cattttaaca actgcctgtc tatccactct attagttatg gatgttacat 60
catcttctgt gttatccccg tcatgtaagg ccttgatgca atgcctgatg gatggggatg 120
<210> 132
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 132
cagctatatt aggaacttcc catgttaact caatggatct cagtacttct gcaggatatt 60
catttgtcaa atctgggtac aaaaagaaag atctaatttg tttagaacca ttctcggtga 120
<210> 133
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 133
ttcattttca agtacaggtc aatgtgaatc agggaactgc tgggagtgct ttggtagtgt 60
atgaaccaaa gccagtagtt gattatgata aggatttgga atttggagca tttaccaatt 120
<210> 134
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 134
tatcacagtt ttttgcttat tttacaggag aaataaatat tcatatccta tatatggcaa 60
agcaggggtt ccttagagtg gctcatacat atgacactga agataatagg aaaactttct 120
<210> 135
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 135
aatgtactac cttcttctct tcagcagcca cagaaatttt ggataatgat ctggttaaat 60
ttatagtgaa aatccttgtc agaattctct gttacatggt gttatattgc cacaaaccaa 120
<210> 136
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 136
tttctgatga gagcccctat gtattgactg acaatagaaa aatgcagcta gcttatttag 60
ataggggttt ctacaagcac tatggcatta tagtaggaga gagtgtatat cagttggatt 120
<210> 137
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 137
ctaatgggtg ccctactact ctggatgggc gtcaacgcac gagaccgatc aattgctttg 60
gccttcttag ccacaggagg tgtgctcgtg ttcttagcga ccaatgtgca tgctgacact 120
<210> 138
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 138
gttggtcgct ccggcttaca gttttaattg tctgggaatg ggcaatcgtg acttcataga 60
aggagccagt ggagccactt gggtggactt ggtgctagaa ggagatagct gcttgacaat 120
<210> 139
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 139
cctggagagt ttggaaaagc taaaggaagc agggccattt ggttcatgtg gcttggagca 60
cggtatctag agtttgaagc tttggggttc ctgaatgaag accattggct gagccgagag 120
<210> 140
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 140
gcagcacgct ctcgctcgtg cgcttcctgg agctcgggct ctcggtggcc tgcgtgtgca 60
ccaagttccc ggagctcagc tacgtggccg agggcaccat ccagtttgag gtgcagcagc 120
<210> 141
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 141
gacctggtga tccagatctc gtgtctgctc tactcgctcg ccacccagcg gctggagcac 60
acgctgctct tctcgctggg ctcgtgcgac tcggacgacc ccgcggtgac ggtcctcgag 120
<210> 142
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 142
tgactttgtt tttctcaaac aacatcaatt atggatgcac atcgtgtata taatccccgg 60
tccgcgctcc gcccacccat cacagcagcc gcggacgctg cgcgccggag cggtccatct 120
<210> 143
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 143
cggtacaaga tggcggacaa ttacgtcatt tcctgtgacg tcatttcctg tgacgtcact 60
tccggtgggc gggacttccg gaattagggt tggctctggg ccagcttgct tggggttgcc 120
<210> 144
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 144
cggaagtgac gtcacaggaa atgacgtcac aggaaatgac gtaattgtcc gccatcttgt 60
accggaagtc ccgcctaccg gcggcgaccg gcggcatctg atttggtgtc ttcttttaaa 120
<210> 145
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 145
ttttcccgcc ttatgcaaat gggcagccat tttaagtgtt ttactataat tttattggtc 60
agttttgtaa cggttaaaat gggcggagcg taggcgggga ctacagtata tatagcacag 120
<210> 146
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 146
agcagaggat tgaaaatgtc tcttctattg acaatagcaa aggaatacaa aagactatgc 60
caagatgcta aggcagctca aatgatgaca gtaggaactg tatcgaacta cactacgttc 120
<210> 147
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 147
aagaaatgga ctacatcaag gaaggaaaag aatccttcat taagaatgag atgggcaatg 60
agcagtaaat tcccaataat agctaacaag agaatgctag aagaagcaca aattcctaaa 120
<210> 148
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 148
gaacacaaca atgtagccct ttgggaagac acggaagatg tttcaaaaag ggatcatgtt 60
cttgcaagcg cctcttgtat aaattattgg aatttttgtg gaccttgtgt taacaattca 120
<210> 149
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 149
tggtggtgcc tcggtgagca gtacgcaggc tggatttgac aaaatcggaa aagatataca 60
gcagcttcgg aatgacacaa atgcagcaat tgaaggcttc aacgggagaa ttgcccatga 120
<210> 150
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 150
gggggaggaa tgctgatggg aatgtttaac atgttgtcaa ctgtttttgg agtcatgaca 60
ttaaattaca gggaggaagc attggccaga aggaactgtt actggactgg gctacaaagt 120
<210> 151
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 151
cagacaaaaa gagcaataaa ggacaagaga aatgagttgg agccttcaga acagagagac 60
ttcctttgtg gaattggaag agcctccaag aaaatacagg aggacaaatg gcaagaatcc 120
<210> 152
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 152
ccgtgatttt tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag 60
tctctatgca atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc 120
<210> 153
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 153
ggcgcgcata tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt 60
gaagctggat acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat 120
<210> 154
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 154
actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca gtgtggacga 60
tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag aaccccgtcg 120
<210> 155
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 155
agaacgtgcg caagaatatg acgctagtca gattcaagtt ttggagggct tagaagctgt 60
ccggaaacgt cctggtatgt acataggttc tactagtggc gaagggttac accacttagt 120
<210> 156
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 156
atctttagta gctattcagt cagatgccta tgaattgcct tacaatctat atgagattat 60
tggagataaa gcggctctct taaaagcaga cggcttggac caagaactaa ttaataattt 120
<210> 157
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 157
gatattacaa aaggtgcttc tattttaggg atgttcgtac tggccgcatt agttcaacgt 60
tgggtatcga ttaagttctt accaattgtc tcacaagtta agttagacaa aggtgcttat 120
<210> 158
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 158
aaaatattat tactcgcaca actcaagatg gtattcagat tgaactgtta aaagactcga 60
agtttgacag tgtgactact ggcaacacca cattaaatac gaatggtttg actattaaag 120
<210> 159
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 159
aaatcgccca ttattacctg ctgaaacgag tgtctggcct gtacaggcgg atggacgccc 60
acaacgcatg aaggcaatta ttcgcgaaga tgaatgcatc ggatgtacaa aatgtattaa 120
<210> 160
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 160
gtcacttagg tcgtaaagac ccaacccgtg gtaacgatct ctacttatca ttagattatg 60
gtttgcaagt tgtcgcgtcg caacaactcg ctggtcgacg tggtgcaatt gttgcgatag 120
<210> 161
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 161
cttcggcaaa gaaccgcgta aagacgtgaa cccggacgaa gcggttgccg taggcgctgc 60
ggtacagggc ggcgtgttgg ccggtgatgt gaaggacgtt ctgctgctgg acgtgacccc 120
<210> 162
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 162
gacgtcggcg tggcggacgc gctgtcgaag cgcctggtca gccaggtgtt ggaaacggcg 60
gtggtggtgg acaacgaaca gatccacctg cgttgttttg aatcgacgca cgagatgctg 120
<210> 163
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 163
cttcgccgcc gcctatccga agcgtaccga cttcctgaaa atcgccgccg gattcgggct 60
ggatacctgc gatctgaacg ccgcggaaga tccgcaggcg gcgctggcgg aggcgatcca 120
<210> 164
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 164
cctagaacag ggttacttat cttaggattt ggttgtgtta atgcgggata tgcttcggtg 60
gtgacatggc tagcaccttt ttatcaatcg ctaggaatgc caagtgccca aagtggctct 120
<210> 165
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 165
gtggatttag agccagcgta ttaaacgatg ctttacaagg ccttgtgatg ctacttggca 60
cctttatcct gctggttgcc attatctata aagcaggtgg cttggatgtt gcgatacaaa 120
<210> 166
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 166
cttgagccat tgcgatcacg agcagatctt atcattgata cgtcagaaat gtccgttcat 60
gagctcgcag aaatgttacg tactcgctta ttaggtaagc gcgagcgtga attaacgatg 120
<210> 167
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 167
gatcatgaaa aagcagtgta ttggtataca aaagccggtg agcaaggtga tagcgatgcg 60
cagtataatt tagcgatttc atatgatgaa ggtattggta tcgaacaaga tcatgaaaaa 120
<210> 168
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 168
tgatggtagc aaagcggtat tctggtatac caaagcggca aatcaaggta atcgagatgc 60
gcaaaataac cttggtgtga tgtatgacga aggtgatggt gtagcaaaag atcaacgtaa 120
<210> 169
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 169
tttatgcggt tgatgtttat ttagccgacc atcaatcgcc tttagcgggg gtggctaata 60
ttggcagtcg tcctaccgta aaaggtaagg gtgtacaatt agaagttcat ttaatcgacg 120
<210> 170
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 170
tgctttctcg ccgccgccaa cggcaccgcc gggcaaaaag cgcagctcaa tacattaatg 60
gaagagatga ttgctctgaa acgccatttt cagcgcgaac gctggataga agtcgatatg 120
<210> 171
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 171
gtgatcattg agcaggccga ccggttaaga aatctggtcg accgcctgct cggaccacag 60
catccgggca tgcacgttac cgaaagcatc cacaaagtcg ccgaacgggt agttaaactg 120
<210> 172
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 172
tttttcgtca gcggcaaggc gatctatggt atgtggaaga agagataagg aacccagcca 60
gcccggctcc gtggcagcta caggggaaaa cccatcatgg ctggtggcaa aattcatggc 120
<210> 173
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 173
ctacttcccg cggtattcct cgtctgcgcc gcgcgatttc acgttggtat caggaacgtt 60
atgacgtcga tatcgacccg gaatcagaag ccatcgtgac gattggttcg aaagaggggc 120
<210> 174
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 174
cgcgcaagat gaggctcgca ctgatggggc ccgcgttcat tgctgccatc ggctacatcg 60
atccgggtaa cttcgccacg aatatccagg caggcgccag ctttggctat caattattat 120
<210> 175
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 175
cgaatggcaa aagtttgcct ggtacctgcc gcgcgccatc gttctgttac tgctgtactt 60
tatccccggc attggccaaa ccgtcgcgcc ggtactgtgg ttcttgttta gcgcctggat 120
<210> 176
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 176
cggtttcttt aacgcctgga gcaacctgaa gcgtctgctt aagcagcgta acgccgcatt 60
gcgccaggtc acgcgctacg cccagctgcg cccgtgggac atggaattaa ttcctcttgc 120
<210> 177
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 177
gagcttatcg ccccgtttgg caacaagacc ctcaaatgcc atcagcacta ttacatctca 60
acgcttcccg gccgtcagtg gccgaaaatt gacgccttta ttggctggct aagagaactg 120
<210> 178
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 178
cagggcgaca gcgactatcg cgccgtgccg tccgatctca acaaatggta tgtacgcggt 60
agcgacagca ccatgacccc gttctccgcc tttgccacca cccgctggga gtatggcccg 120
<210> 179
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 179
gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc ttctcggttt 60
tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctga tgtttgaaca 120
<210> 180
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 180
gttcgcgtaa agcgtatgct tattaagcgt gtcgcgacga tggtgcgcga tatgcatgct 60
gcgggcatta accaccgtga ctgttatatc tgtcatttcc tgctgcactt gcctttttcc 120
<210> 181
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 181
tggcggggga aacacagctt gatcaagcgg ttatcctgat tgcagcctgt aaagccattg 60
tcactaacga ttctggcctg atgcacgttg cggcggcgct caatcgtccg ctggttgccc 120
<210> 182
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 182
aggcgttcaa acgcaagacg ttattaaaat ctccgcaaca tctggtttaa caccagcacc 60
acaagcgaga gatcataaag ttgaaatcgc aaaattaatt gatgtttcaa cttgtatagg 120
<210> 183
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 183
gtgttggggt ttatgacaat ccagttgatc ttgatgcaaa agcatggaca gtgatgcgtt 60
ttaacgaagt tgaagaaaac gatcgtttag aatggctgat tcgtaaagat ggctgtatgc 120
<210> 184
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 184
ggtattgtag atttccaatc cgataaatgt attggttgtg gttattgtat tgcaggctgt 60
ccattcaata ttccacgaat gaatcctgaa gacaatcgtg tatacaaatg tactctttgt 120
<210> 185
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 185
ggtgcctgtt ttgggtatct gttatggttt tcagaccatg tccgaacaat taggcggcaa 60
ggtagaagct ggattggtgc atgaatttgg ctatgccaag attgatatca cgcatcaaga 120
<210> 186
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 186
tgataaaagc gacagatatt gtggttggca caggcaaaac tgccgttaaa ggtgctttga 60
ttatcactga atacacaggc tatctgatgg atggcacggt gtttgatgct tctgtgcacc 120
<210> 187
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 187
taataagggt attgatttat acgcaactga ttctaaagct gatctaatta atatcgctaa 60
taatgcccct ggctcaaagg tatttgtccg tattttagtg catggtgctg acacggccga 120
<210> 188
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 188
cattgcgacg agtggggacg ggtgaaggtc cagttcccct gggatcgcga gggacgtcac 60
gatgagttca gtacctgctg gatacgggtc gcgcagaact gggcaggtgc cgactggggg 120
<210> 189
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 189
gccagctact ggagccagcc ggccggttgg ctggtggcgg cctgcgctct gctcggttcg 60
gcctgcgcac tgttgtctct gagcgggcgg atcggtcgca cgcgtcgcca tgcgggcgtg 120
<210> 190
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 190
atgtggacga tgacgccttc ctcgtgcaga gcgagaaggc cgaccgcggc atcttcctgc 60
agatcgtcct gaagggcctc ggcggcatcg taggcaacaa gacggaaatg ttcctcgaca 120
<210> 191
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 191
gcagcgcgta tgcgtgattg gttagaagga tcaacattaa cgacgcgaca atcagaaata 60
cgtgtacaag atgcatatac gttgcgttgt ataccacaaa tccatggcgc gagttttcaa 120
<210> 192
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 192
ttctaaaaat gatgtcatga cgttatttga agatatcgcg caagtctttt taaaggtaac 60
actatcattt atgcaatacg gcgcattacc agagttgcat ggtcaaaata tattgttgtc 120
<210> 193
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 193
tgattaatca gtctatatgg cgcagtaact ttcgcatttt atggctcagt cagtttatag 60
cgattgctgg actgacagta cttgtgccat tattgccaat ttatatggca tcactacaaa 120
<210> 194
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 194
actatcttta caacaaagtt gacacaattg ttcagaaggt ttcagaatta caggagttga 60
ttccagaggc ttcgattgga tatgttcatg gtcgaatgag tgaagtccag ttggaaaata 120
<210> 195
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 195
gactatgcct tggaaactgc tagggctgaa gaccttgaac aaggactaga gcgtgggaaa 60
gttgaaggaa gggcagaaag gaaacttttt accttcctag acatagtacg ccaaggtctt 120
<210> 196
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 196
aggtgagatt gtgatcattg atgacgaggg cattcagtat gacagctata cagatgatac 60
ccagttggcg gtttgttcta tggagtatat ctactttgct cgccctgatt ctaatatcca 120
<210> 197
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 197
cccatgcttt cgtcgtcggc agcagcttcc tgcaaagccg taatctgttg gcgatcgtcc 60
atccgatttt gcgtcagctg atggaagatt ccggtgaaac ggttaacctc gcggtgcttg 120
<210> 198
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 198
ccagcatgta tccggatcaa tcgctctatc cggcgaactc ggtaccggcc gtggtcgatc 60
ggatcaacaa cagcttccgc cgcgcggatc agatccagtg gtctgcgggt attgagccga 120
<210> 199
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 199
atatcctcta cggtatccct aaacagaccc gctctgagaa gatttccggg gtctacgaac 60
accatatcct gcaacctcat gccttgttaa cccgcggctt tgacgatagc tttctggccc 120
<210> 200
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 200
agcaagctac tcagatagca agggccatgg ttacggaata tggtatgagt gaaaagcttg 60
gaccagttca atatgaaggt aaccatgcga tgatgccagg acagatctct cctgaaaaag 120
<210> 201
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 201
ggcaattatc atttgtctat catttatgag ccaatataaa gcttttactt ggattgcttt 60
tgctattgcc acaattattg gaacattaat ctacttagct tatggttata ctcattcaat 120
<210> 202
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 202
ctcaagcagc tggttatcgt actggttcaa cgccgatggt aggtgcgatt gccgtttgga 60
acgatggtgg ttatggacat gtcgccgttg tagttgaggt tcaaagtgcc tcaagtattc 120
<210> 203
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 203
aaaaactcca taacccaaat ggtttataaa cacgccattt caacagttgt tccttctcga 60
atggtcaaaa ttcctgctga ggaaagctct ggtgaggaag aaggaactgt ggcagactaa 120
<210> 204
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 204
ggattagctg gtgcggcaat agccaaggga ggcctgtctt cactcaagat tacaggtttt 60
gccaaagtag cggcagggat atttatatct cccgttctgg gattattgat tggcttatta 120
<210> 205
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 205
ctttgtctac ttgcgcagca cggggctcgc cacaaactct ctgtcgagca ttctgcagta 60
catgacacgc atcattgtac ttacggaaag cgcgggcatt gccctgggca ttctgggagt 120
<210> 206
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 206
tattagtgaa gacattgatg atgaggactt aattacccag gaaaacgtgg taatcacgat 60
gtccactaac ggttatctca aacggattgg ggtagacgct tataacctgc aacaccgtgg 120
<210> 207
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 207
tttaagacca ctaactttaa accaggtgtt attctttatg atgtgggggt atcacgtaat 60
gtcttaaaca aactgtgtgg tgacatcgat cctaatggga tagagaaagt tgccaaatgg 120
<210> 208
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 208
gcttatgcaa aggtgcaaaa caacttaggg gacatgtcgg gttacattga agaagtgttg 60
tcgaaccata aggtagttca tgtcttgaaa ctgcaagagg tgatgattga taactttgac 120
<210> 209
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 209
agtatgtaca ggattgccag gatcattgcc tgtattgaat cagagtgccg tggagaaagc 60
tgtgcttttt ggctgcgctg tcgaaggtga aatctctcta ttgagtcgtt tcgataggaa 120
<210> 210
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 210
ttcaaaggga gtctatgttg cgcgctagca agcaaagcaa aaccctattt tgaagaggat 60
cccagattcc aagattctga tacgcaattg cgagctctga ctctaaggtt acaggaggct 120
<210> 211
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 211
tgaaggcaat acgataggag ctacaggaac cgtctctcat agtgctattg atttaggaac 60
tagcgctaag ataactgcgt tacgtgctgc gcaaggacat acgatatact tttatgatcc 120
<210> 212
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 212
attcaagctc tccaccccac tccttaaaga aatggtgcgc attgggctgc cttctggatt 60
acaaggatcc tttatttcta tcggaaatat ggctcttcaa agtttaataa atggattcgg 120
<210> 213
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 213
attcgactac tcctatttga ccaacggaat actagctcaa aagtcgcctg ggttgcggtt 60
atttttattt tgcctgtact cggaacgatt agttaccttg tgttcggtag aaatccagca 120
<210> 214
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 214
tgttggtaca tacggcggtg cgtcgattcc tgactttaca acgggagcaa tcgtcggaac 60
agtatttgcc gttttatcag gtcaggatgc ggaatttgcg attggccttg caattccagt 120
<210> 215
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 215
catccactac ctctcgctgc acgtcgaatc cgcgcgcacc gcgctcaagc agctcctgcg 60
ccaacccttc ggcacactgc ttaccctcat gatgctcgcc gtcgcgatga ccctgccgct 120
<210> 216
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 216
caagactaca cagcccgcgc acaagtttcc gaagccattc ttttggccga aggtcaaaaa 60
tcagccgtta ccgagtatta cctgaatcac ggcgaatggc ccggcaacaa cacttctgcc 120
<210> 217
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 217
acagttctgc cggcgtggca acctccgccg acatcaaagg caaatatgtt aaaagcgttg 60
aagtcaaaaa cggcgtcgtt accgcccaaa tggcttcaag caacgtaaac aacgaaatca 120
<210> 218
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 218
ggaaatgacg atggaacaag agccactgaa cggctggccg caaccctatc ggttgctcga 60
tgcccaaccc tattgggatg cgctgcaaga cgagaaactg acataccagc agtgtgtcgc 120
<210> 219
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 219
cgccaatccg tctggccggc ccattcgcgc tgcccgcatt gttcgtccga ccaactggtc 60
tggcaagcgt ccagcggacg cgcaacggtg tactccttca gtacggtgac gcgcgggccg 120
<210> 220
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 220
gtgtgggcct ctatcgttcc ctataccgtg ggtttcgtcg aaatggacga agggtacttt 60
ctgttcacgc agatcgaagg gccgccaggc tcgattggcg tcggagacaa agtgcaggtg 120
<210> 221
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 221
tcaccgccgc aatgatgctg gtcttcttct tcgtcgcggc gatcgccagc tacatcgccc 60
acggagcgct tcgtgacacc accaatcagt tcgaaaagcc caccatcggg ctgtatctgg 120
<210> 222
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 222
gaggagttcc tgctgaccca tccggacatc gaggatgtcc acgttgtggg ggtacccgat 60
gagaagtatg gcgaagagct gtgcgcctgg gttcgaatga ggccagaccg tgtggtcatc 120
<210> 223
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 223
gttagccgtc gtttgctcac ccgagacgaa ttcgtaccgg cgacaaccct caacgcactt 60
gcggcggcgt ggattcagtt cgaggtgcac gactggttca gccatgggac gaccgacagt 120
<210> 224
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 224
cctgcagcag gacgtgatca atatggtgaa cgaccccttc cagacgctca ccgggcgtcc 60
gctgatcggc aacggcgcca acggcactcc ggggaccggg gctgacggcg gagccggcgg 120
<210> 225
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 225
aacagacact gctatcggcg cagccctcgg catctccgcc ggcacctacc ggcggctcaa 60
acgaatcgac aacgcaaccc gcagcgagtt ggcgcgtggg cggcccggca cccctaagca 120
<210> 226
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 226
cgcacactgg attcttgtgt gcgagacggc ctgatgtcgt cgcgggcggc ggagaccgcg 60
gaaaccttgt gccgcaccgg gccgccgcag tccacctcgt gggcgcagcg ctggctggcg 120
<210> 227
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 227
acttgcagtc cgaagtcaat cgcatcgccg cgctgctgaa cgcgcggccg atccaggccg 60
gagtcgtgct gaaggatgat gaccgcaaca tctatatcga tgacgacggg cgataccact 120
<210> 228
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 228
gcaggcagaa ggtcgatcac gtcggcgaca tcgacgaggc cctctactgg ttcgcctgcg 60
atatagcttt cgacatcggc ggttcgtcct ccgcaagaca ttgtccgcga gatcaggacc 120
<210> 229
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 229
cgacggcaga ttcgaagccg cgcaggtatg tcgatggctg ctgacctcgc ggctcgaact 60
catcgaagac cgcaaccgat acccgatcgc cgaggaagtg atcgattcgc cgatgttcgt 120
<210> 230
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 230
tcgcaaggtg tctccgtcac cgtcattgac ggcacccccg atctcagcag ccaccggcgg 60
gtattgaagg gttccgacgc cgtggtgctc aactatgggg gagacgcctg ggtgatccgt 120
<210> 231
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 231
agggttcggt tcgatctcac cgtagacctg ctggtgccac tgccgggatt cgtcgttcga 60
cgaggcgcca aaggagtgat ggacactgct acccgaggcc ttcgtgaccg ggtgttagcg 120
<210> 232
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 232
agcgtgggtg gtgttgttgg tgatccggtg aatggcatca cgctgttccc gggtggttat 60
acgcttcctg ggattccgtt ggacatcaat gtgggtggtg ttgtcggtga tccggtgaat 120
<210> 233
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 233
gacacgaaat acaaaatctg ctcaggtaac gattgcgcca tcttgtctga ggtgaacgcc 60
accatcttca gccatctgat cgataaatcg gtggatcaga tcgagcggct gattacgggt 120
<210> 234
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 234
ttttcgcatg gctcgagcag caatgcccga gatcgcaatg gaatcggtca ctgcactcat 60
gcgaacaaaa aatcgacgcg gccgggagcc aatccggatc ggacaggtca cgccctcggt 120
<210> 235
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 235
aagccattgc ggcatcgtac gttccgctgt tcagaaagct actcagaaat acccgtctag 60
aggtggatgt cacccgggtc aggtttctca ctcccgacgt agcgctgatc catgcccatg 120
<210> 236
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 236
accgagcgtg gcgcgctagg gactgaacgc ccaagtaaag cagagggctt cgtcgtttcc 60
catgatgcga tgcacgattc gcggacggtc ggtgccgttc tgatcagctc gggcaaagtg 120
<210> 237
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 237
ctgccgttca ccggattgcg tgacccgcag ggtttgtcgg tggattatgg cggcacggtc 60
tacgtcggtg acactctgca caatcggata ctggcgttgt ccgccggctc aaccaccccg 120
<210> 238
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 238
gattccagtt gagtggcgaa acatggtcgg gccaattagg cgtcacagcg cgaccgacaa 60
actactcctg ctattgccgt cgacgccgat cgtttcgtct gacgacgtcg catcacttat 120
<210> 239
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 239
ttgtgtgata cctaccataa gccttttgta atcactggtt ttgagccttt agatatccta 60
caagcaattt taatgttggt gacacagatt gttgaaaaac gctgtgaagt ggaaaatcag 120
<210> 240
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 240
gttaatgaat atccataacg ttgaagtatt cttagggatc ttcatcggtg cggtaacatt 60
cacgggttct atcgtagcat tcggtaaatt aagcggcaaa atcaattcaa aagcattaat 120
<210> 241
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 241
cttagccggt ctttctaaac atgaaacggc aaaagccggt tgttggtatg gtattgtcgg 60
tatgacaatt gcccttgtcg caaccatttt cggcccgcaa tctgaaggca cattatggat 120
<210> 242
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 242
tgcagcgctt tattgacttc ggtgtactga ataaaagcac gacagacatc gataaccaga 60
accgtaagca tcaacaacac tggcaatgcg agaataaatt caataatcgc aagcccttct 120
<210> 243
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 243
gccaactcaa gcccgttgca aaagcgatgg ggaaaagtga agcgtccatc agtaaatacc 60
ttgcccgatt acgacttcag cttaacgatg agctgtttat ccgacatcct caccacatgg 120
<210> 244
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 244
acgaccctta cggcgcaggt aatgtggact tgtacgcaag ccgcaaacac tggccgacag 60
cagagcaaca tgagtacgcg tcaaactacg cgggcagcaa tgaatacctt gctatcccag 120
<210> 245
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 245
gtgtccaatt gacaagaaac actacatcca agacgtgacc cagcaatacc aagatatgtg 60
gtggttacgt aatgcctcgg gtcatgccaa actcgaacag atgaacgtaa tggtgcgctg 120
<210> 246
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 246
cgtgtgtgag ttggcgctgc tcgattgccc attggcgcag gttgctaact ttgtgcaatt 60
gctaggacga cgttttgtgt ctgcgcaggt atgcatggta ttggcaggag acttaaccga 120
<210> 247
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 247
gaaccgagtt atcccaggtt ggacggaagg cgttcaattg atgccagtcg gctctaagtt 60
caagttcgtt attccacctg agctagcgta cggcgcgcaa gacacgccaa gcattccagc 120
<210> 248
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 248
atggtgatgg gactacttcc agtagtgggc gtacctctac catttatttc ttacggagga 60
acaatgattg catcaatgct aataggattt ggacttgtaa tgaatgcgca aatacacgga 120
<210> 249
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 249
gagtatggga ctaatttatg aaggtacgtt acctgctcct aaaggtaaag tgcatgagga 60
ctggaagcac aaagtacaaa aattgttcaa atctaccaat tacggtgata gccaagatcg 120
<210> 250
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 250
catggagctc attataatcc gtattttcca ggtgcacaaa tcgtaatgcc gccaccgctt 60
acagacgggc aagtaactta tatggatggt actaacgcaa gtgtagagca gatgagccga 120
<210> 251
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 251
ttcctggaag tggctgcgaa gaccgagaag tgctcgcacc cgctgatccc ggactaccag 60
tgcctgcagg tgcgcgaagt gaagttcgac gacaagggcc tgaagcaggg cgagccgggc 120
<210> 252
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 252
gacggcgtgc gcaacgtggt gcgcgtgaag cgctacgagg tgaagaaccc gccggccgat 60
gcgccgtcgc aggcgtacgt gctggacatg gtggtcgagt cggccatcga gaagaaataa 120
<210> 253
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 253
tgtccgaccc cgcacccccg ccgtccctcg atgccctggg cacgccgctg gcctgggccg 60
gggccgatgg ctgcattgcc ggctgcaatc cggccttcgc ccgctggctg ggcgtcagcg 120
<210> 254
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 254
aggacatttt tacttactac gacgatctaa tatcccaatg gctgaacaat gttcgcttaa 60
atgcccttac agcaccatat actgtgcttt tttcttcatt attatttcat gatcatcttg 120
<210> 255
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 255
atttcctatt gtagctttgg gcgttggagt gggttcctat attacaggat ggatttcagg 60
aaaagatatt aagttggggt atgtgcctgt catgacctta gggttaggct ttgcctttat 120
<210> 256
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 256
gctttacaac ttgctggata cactgcgata cctgttgttc tcaaacaagt ggttgcagac 60
gatatagctg cagaagctac tcttatcgaa aatatccaac gtgtcaatct cagccctatg 120
<210> 257
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 257
aaagctacaa tcagcaagta ataactcctt tacctgctgt tacctcagac ctaaatccca 60
aatatagttt tgaaaacttt attcagggtg atgaaaatcg ttgggctgta gctgcttcta 120
<210> 258
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 258
gctaatacac caggaacaac ctataaccct ttgtttatct ggggagggcc tggactagga 60
aaaacccatt tgttaaatgc cattggaaac tctgtgctat tagaaaatcc caatgcccgt 120
<210> 259
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 259
accagtgacc gcacaccaga tcaccttaat gatttagaag atcgattggt aacccgtttc 60
aaatggggct taaccgttaa tattacgcct ccagattttg aaactcgagt agctatctta 120
<210> 260
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 260
tcgagtgtgt tccttctatg cattggtccg gtagatatgt gattgattct aaccaatctt 60
tgtggtgctc gtatgtgatt aaacgtaacg gagaatcact agtgtatcat gcgggagata 120
<210> 261
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 261
agagagtgaa aggtcaagat caagcaattg aactaatctc tgatgcaatc aagcgttcta 60
aagcaaacat caatgaccca aatagaccaa ttggttcgtt cctattttta ggaccaactg 120
<210> 262
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 262
gtggcttgag caatgtagaa tatggcgaat tggtaaaatt cgaatccggc gttattggaa 60
tggccttgaa tcttgaagaa gatttagttg gtatagttgt catgggtgat gaccgtaaca 120
<210> 263
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 263
aaaatacttc aaacttagtt agtcaattag ttcctgaagc atatactgca tcaggaatta 60
gtattgctat ttctgttttt agtgttatag gtacaatcgt tattgcttta agtgtattac 120
<210> 264
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 264
ttttaaagta gatgaagccg cttttttagg tggtattgcc gctgcctata tgttaaattc 60
aaatcaatca gtttttggca aggacaacaa actaacttga ggtggatatg ttgctttaaa 120
<210> 265
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 265
attactaaag gcgttgattt aattttacca gtagctatta ctcaagttgg tgttgctgta 60
actgaagcaa tcgcaactac ttcacataac gtaggtgtta ttggtgttga cgtagaagtt 120
<210> 266
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 266
aggtagtagg tggttatgca ggttcagctt taatgagaga cgagcagtta gctgctaaga 60
tttttgaagc gaccgttaaa gcagttaaga ttccagtaac ggttaaaatg cgtattggtt 120
<210> 267
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 267
ataggagtag gttatagata tggacctgag catagttgtc ctactccgga tgataggtta 60
agttctaagc taattgatat gagcaataaa aacaccaaga atgttaaagg gctggaagaa 120
<210> 268
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 268
caattgatac gatgctatcg agtgagcgtg agcgtttgct tgtgattgag cagaagctgt 60
gtgaatcagt tataggacag gatgaagcaa ttaaaggtgt aagtgatgcg gttagaagat 120
<210> 269
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 269
tattattggc cctgaagctg gtactttaat tgctgaagct gctgtagcga tggaatttca 60
tgctgctgca gaagatatag ctcgtacttg ccatgctcat cctactctta gcgaagtatt 120
<210> 270
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 270
ccatgcctat tatgaagaaa agtcagtatc gactacaaat gacttatccg attccagaaa 60
ccagatcctg caagagcata ggtcaaacag aagctacatg gcaagctggc aaagaatttc 120
<210> 271
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 271
ctgcaggaat tcagtctacg cttggaaaga atattacttc gctacaatat ttaaagcctg 60
gaggtagttt tagcatcaag aaatggttta gtaattcgaa tgaaactggc tggctattta 120
<210> 272
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 272
ggccgacgag tggtcagccc gcgaacagcg ctgggcgacg ttgcgtgacg gctctgagta 60
cattcgcaac agcaccgaag gccatgccct cgacctgctc gaaagcggct tccccgactt 120
<210> 273
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 273
ttcaacgaca ccgggtacag cccgctggac gtcctcgacg acttcgccgg gttctacttc 60
gacaccgcgc tttcggcgag cgcggccacg ctgccgaccc tgctcgcgtt cgccgaaccc 120
<210> 274
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 274
cacctacctt ttcctgccgc ttcaattcat cggcctgctc atcgcgggct acctgtgggc 60
cagccacgag ttgagcctgg ccgacaaact cggcttggcc gtcaccctcg gcttcgtctc 120
<210> 275
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 275
cgccgcgaaa acgatcaaat acctgctgga cgaactctcg tccggattca aatcgcttga 60
taccgacgtg gtttcgctga ccaaaacctg gcagggctac cagggcgggc tgttcgcgca 120
<210> 276
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 276
tcgtctcaga agtaccgctg cactacaaac gaccggatgt catcgtttac cgctgtatcg 60
aagaaccccg tgggcgctgg aagttgaagc cgaccatcgc ggaaaccatg ctggtggtcg 120
<210> 277
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 277
tgtcactgcg gacatgatcg acaaacgggc cgagtacgcg cgattcggca tcgaaaacta 60
ctggatcgtg cgcatggccc gcgacgacgg tcccgcgatc tcggtcgaga tgctctactt 120
<210> 278
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 278
tcggatttcg actccaaccg cagcggaggc atcgtcgcgg tgaacctgcg cgacaacgac 60
gaactggtcg gtgctgtgct gtgctcggcg gacgacgacc tgctgctggt gtcggcgcag 120
<210> 279
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 279
tcatcgccgg catcttcgcc cagacctgtg tgttcggcgc gaccttcacc gggctgagcc 60
tggtggagga catgcagaag ggcatcatcg accgattccg gtcgctgccg atggagccgg 120
<210> 280
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 280
gttcgagacc tattactccg agcatccgaa cgaggcgttc gcggcgcagg tgtggaccaa 60
taacgcctgg gtgtcggcga tcgccctgtt caccggggtg ctgatcctgc ccgcgctgta 120
<210> 281
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 281
ccgaccggct cggcgtcgcg gtgagcatcc tgggccggat gcgcagcctg cccggcggag 60
acgccgagct ggcctcgcgc cccggccagg gaacgacggt gacgctgtcc tggcacgacc 120
<210> 282
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 282
cgcgccgggc tgggagccgc gcgaactgcc cgccgccgac gaggaccggc tgtggacgct 60
gctgcgccgg atggcccgga tgtcgtatcg caaggcgccg gtaccggtca cgctggcaac 120
<210> 283
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 283
ggtggacgag gagaacgtcg gtgaaccgac cgggttggct tgctacgacg gcgacggcgc 60
ggtccggttg tacgccttcg atcccggcac cggtgccatg ctgctcgaac gggcgcggcc 120
<210> 284
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 284
accgccatcg tgacctcggt caccaagggg ctgatcgaac ccctgctggc gattctgggc 60
accaatggcg aactgggcct gggctttcag atcatcggcg acaaacccgc caccttcgtg 120
<210> 285
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 285
ggggccgatc atcagcgcgg ccatcaattt cgtcatggtg gcggcggtgc tgtacttcgt 60
gctcatcctg ccgatgaacc acctgaagaa gcggttcgcg accccgaccg aggtcaaggc 120
<210> 286
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 286
tgtcctcgcg gtaccggagc gactgtccgc catggtggac gcgctcggtt cgccgctgaa 60
tattgttgta cccgaacaga tcacggccaa cgtggcggag tatcgcgcgg tgtacgatcg 120
<210> 287
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 287
ataatagaag agtttggtgg aaaggatagt agtgtaaatg ttcaaagata caaaggtctt 60
ggagaaatga acgcaggaca actttgggaa actactatgg atccagaatc aagaatatta 120
<210> 288
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 288
aacatcaaca cactcaaact tattattctt tacaactaag ggtaaggttt ataagataaa 60
agcttatgaa gtaccagacg caggaagaac tgctaaggga acaaatcttg taaatatatt 120
<210> 289
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 289
cagggccagt tagacctaaa aaaccaaaaa gatcaacagc atatacacta tctttatatt 60
taggatggag tagtattttt gcagcactat acttttttat tgtaggatta cctttttcaa 120
<210> 290
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 290
tgaaagattg gtttgctcag attgggctta acttgattga cttcaagttg gagttcggat 60
ttgacaagga tggcaaaatc atcttggctg atgagttctc acctgacaac tgccgtctct 120
<210> 291
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 291
caacaaattc gtttaacagg acaaggtgaa gctggcttca atggtggtcc ttatggtgat 60
ctctttgtta ttattaacgt tctcccaagc caacagtttg agcgtaatgg atcaacaatt 120
<210> 292
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 292
agtagaaaac aaaactttga catcgtagtt tctctttggg gtggggatta tccagaagga 60
tcaactttct acggcctctt taagtcagat tcacaaaata acgatggaaa atttgctaac 120
<210> 293
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 293
tcggcctgtt accaccaaag gccggggaga tccgcattga taacacaccg ctgcgtgact 60
ggagtgccgc cgcactcgcc cgccgcgttg cctatgtgcc gcaggcgcac aatatgccgt 120
<210> 294
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 294
agagcagcat tgatcagcac cagacattgc tggtctattt tgatgaattc ggtgattccg 60
ccctcaatat tatggtgtac tgctttacca aaaccacggt ctgggcagag tggctggcag 120
<210> 295
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 295
gttaagtagt gtgcggttat ttgcggcgtt ctcttttttg ccgtttctga atgataaatt 60
tatcctcagc cggaccacgc ggggcattat tatttttctc atggcgcagt gtctcagcgg 120
<210> 296
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 296
gaacccaacc accatccgtg gcgcagttgt actaacaaag gcggcataca acaagtacgc 60
ggactaccgc gaccgcaagg ccagtgaggc gtacgacgct ctcctgtccg cagcagatac 120
<210> 297
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 297
gatgtctacg ccctaggctg tgtgctttat gaaaccctca ctggtaaacc acctttcgag 60
ggtgaaaccc ccttcgctgt tgcgtaccaa cacgtacaag aagaccctgt aaagcccagc 120
<210> 298
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 298
gcgataaacg tcgatgctgt tgtgcttact gctatgtcta agcaccctgg tgatcgttac 60
cagaccgcac aagaaatgtg tgcggatctt gaacgccttg agcgcaatgc ggtcactgac 120
<210> 299
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 299
ctgctgggtc agtgcccgga tagcatcaac gccgcgctga tttgccgtgg cgaaaaaatg 60
tcgatcgcga ttatggcggg actcctggag gcgcgtggac atcgcgtcac ggtgatcgat 120
<210> 300
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 300
aaccgttaaa cgacacgcgt ctgttggcgc tgatgggcga gctggaaggc cgtatctccg 60
gcagcatcca ttacgataac gtcgcgccgt gctttcttgg cggtatgcag ttgatgattg 120
<210> 301
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 301
atggctgatt cccctcatcg cgttattgtg ggtcgctggc agcgtattca tttgcctctg 60
gcatattgag caaatgcctg gcgttatcgc ctcgatagtc aaaagcgcat ttggctggca 120
<210> 302
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 302
gtgatgctcg aaaatatcgt ccgtcatgtg gaggaggggc ttgcgccatt tcaggccgct 60
attgtcggca gccaggaagt gacaggcaca atcatttcca tgtcgctgtc actggtcgcc 120
<210> 303
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 303
tgatgcgctt tgtgaaacgt tccgcctttt atctgcttgt ggcgctcatc attctggttg 60
cggtgtttcc gttctattat gcgatcatca caagctttaa atcgggaacc gcgctgtttc 120
<210> 304
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 304
tccgcttttg cgcgtatgcc caagccggaa atgcggcata tggacctgcg cgaagcattg 60
cgtgaagcct cgttcctgat tgaagtcagc cgcagcgaca ttcatttcga caatgatttc 120
<210> 305
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 305
tcgagtgtgt tccttctatg cattggtccg gtagatatgt gattgattct aaccaatctt 60
tgtggtgctc gtatgtgatt aaacgtaacg gagaatcact agtgtatcat gcgggagata 120
<210> 306
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 306
tggcaaaggc agtcttcgtc acaccatgaa gacgatctag ccagtataga gatacccgag 60
ttacaccaaa ttcaagacag agctccagat tcattactgg ctcattcgga taagcgatct 120
<210> 307
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 307
tgggtattgt gttgacggac tgtctgacat gagctcttgt tacaatgaat atcaaaatac 60
tgtgttcaat tggcaggttg ttagtccgaa cgtatatacc aatcctcaat tagaattcaa 120
<210> 308
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 308
gaaggtttga ctaatgcgaa cagaccagta agaagggtgg attgtacttt ggccacagtt 60
gaccacaaca tcccaaccga gtccagaaag agtttcaaga acttggactc tttcattaca 120
<210> 309
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 309
ggaacagaac gtcaagcaat ttggcgtacc atactttggt ctaaccgaga agagacaagg 60
tatcgtccat actattggtc ctgagcaagg tttcacctta cctggtacca ctgtggtgtg 120
<210> 310
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 310
atgagtggat gagcaagtta tcactagttg gcgctcatgt tagtgacgta acaactgtca 60
agatctttcc aagtaatgct gtgctcctat cagggtctac tgacatgcag cttaaggtgt 120
<210> 311
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 311
ggtataggaa gtcgaccata atgggatcac tccaagacta ttattatgcg atcgtcgttg 60
acctaaagat gctgccgctt gacctgaaag gagtcgttag tgggatagca accaagttac 120
<210> 312
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 312
gcgccaatat aaggacaggc taaatgcacg gtattatgag cctgccatat tgactctgaa 60
tgtcagtgat aagcatgtgg ctccgattgt tgaggcgttg attcaattta cacacttgaa 120
<210> 313
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 313
tcaaaaccat ttccagatct gccgacgcat atgttcctgt ttccaacacg agagagtcgg 60
ctcttcctag aaacctcaat cccgagctgc atccttttga aagggcccgt gagtatacca 120
<210> 314
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 314
catgatcgag agtttagatg caccgagttt gtgccttccg gttccggtta cgacaattta 60
cccgatgtga ataagatatg ctcgacggtg ggatctaagc cgggaagcca tattgtcaat 120
<210> 315
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 315
atcattaacc tactgttcat cacaaaactt gggctcagat tcaacttcgg atcagtacat 60
gtctaatggc tattgtcaag attattgtcg taaacaaggg tatgccgtgg cgatattatc 120
<210> 316
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 316
actccaagag ctcacttagc attgaccaag tcgagccacg tagcagctca attcagtaca 60
acctgtcgtc cgatcaagtc caagacagtg ggagctccct tggattcaat gacaagtttt 120
<210> 317
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 317
attcactaaa ttgatggtta gggagccgta caacagatct ttgatccaag ctgtgctcaa 60
tggtacattg aatgcaagct taacgggata tttggtgagc ttgcaggatc aggaatatac 120
<210> 318
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 318
aaccacaatc agttcccgtg caccgcgaat ggattagtga ttatcgtgtt ccccatagtg 60
tattgaaata caatctatca agagaagggc ccggcaatag aatcatcaat accgatatag 120
<210> 319
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 319
cacatatgat cctattctga atttaatctc aagtggacca tttccaaatc tcatgagacg 60
ttataagttt gtacatcaag caagaatggc tggtacagta ggaattttgg tgaacacatt 120
<210> 320
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 320
gaaccgggag tgtctatcat gcgtgaaccg gcctcaacgg cgtcagagtt tgggtttatg 60
aatggtgttg gcgcgtctat gtgtggacct aaacggtggg ctaccgcagc taggtggtaa 120
<210> 321
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 321
cagtccgagt atggccgctg gtgcttttag cggctcgacg gggccggtga tgactcctac 60
taccggacaa agtcagttag agtcacagac cctcgttctt agtgaggaga ggcagcccct 120
<210> 322
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 322
atccagaatg cgcaacttcc cactcacaag ccgtccattg attacacgcc gctgcctcac 60
cactctcggt ctgcgaaggg aagacccctc caggatatgg gaaaggcgca ccccacttac 120
<210> 323
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 323
accgtttcca agcttgtcta cacctcatct ggtggcgtga tctactctgg aaaggaggat 60
atttgtaatg cagacgagag gttgtattat cccgctgtcg cgttggatgc gtacaacgag 120
<210> 324
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 324
cgatacaacc tctctggtac gagaggggag gaaggaagga tactggcccg tgggagtgtc 60
agctacgcac ctatcttata tcttgcttaa aggtttggaa acggaaccat ggttccctag 120
<210> 325
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 325
agccaccatc tactcgtccc gtcacacgac gtcaagtgct gacgcgctcg cgctcaactt 60
cgtataacat gtcctctctt ggcacgacta cccgtcgtaa ctccactttg tcagcttcat 120
<210> 326
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 326
aggtcacttt tacccgccgc ttggaatgtc tcaccagctt ggtcacaacc taccaaagca 60
gcaatcaaca tgacgaggct tacagcgttt cgagacagtt ctaccggcag acacttgaca 120
<210> 327
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 327
ctttagaatt ggataccgcc ctattgaagt tcggtcggga ccttgaggaa ctagggttgc 60
ccactgaatt taagaccgtg aatgacttgc tagctgtcat gaccgcacta caagagaagg 120
<210> 328
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 328
gttcttatgt tgacctcaag aggtgtgaca tatcggtatg ttgagggatt tcgcgaactc 60
ggacttactt ttcagacact gacgagcgat tctagccacc ggcacttgct ctccgatctc 120
<210> 329
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 329
attcgagttt cttgattggg cgagcgagga tttcctagta taccagaaga cgggcgagtc 60
tgataaagta gttgtgttta tcaatctatc cactagggct aggaagccgc ctgtggccac 120
<210> 330
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 330
aagtcactat gtcaaagcgg cggactttgc agaagacgta ttctcatgtg cccgcagtgt 60
gatagcagat ggggcgcagg ggcgcttggt caagcgatat gaggagatgt tgatgccagc 120
<210> 331
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 331
ttataacggc ccttcggtat ctgatgtacc ccgacatatt cccagttatg gtcacccatc 60
cagcacaatc aatgtttctg gggaccttcc caatgggcct tgccactatc ataaacatgt 120
<210> 332
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 332
ccaggtttct gtacgatgcc ttacgattca tcttgaagtg tcgacgcata gctgatatag 60
cgccacttca gctatattgt tcagggttga tattcgcgcc atcacattca attatcagac 120
<210> 333
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 333
cgaatgggtg actctacatg gcaagagaat actttggcta cccactgact atcgtgtaag 60
gcgcatgatt ataagagatg gagtacttgc tataggacat gggatgatgg gtgcgaagcg 120
<210> 334
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 334
tcctgcggca tattagcatg ttcgcaattc agtactcatg tggtatgtcg tggattgact 60
caggggtgaa gccccaggcg ctttgcggcc actcactcgg cgagtgggct gcactgacag 120
<210> 335
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 335
tcaataagtc cgtgccaatc cctgaaacgc ccgcaatcat gtcattcgat gcggccggca 60
tccacccaat cccgtccaac tcccaagacc gactcctcag cgcatcatgt ctagatttcc 120
<210> 336
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 336
actggaatag cctggttcta cacgggcact ctggttggtc ccactgttgg acccctgctt 60
gcgggtgcga tcgtcactta tacaacatgg agggtgattt tctggctcca gacgggactc 120
<210> 337
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 337
tatagtagag tcagtatacc gtacaagctc aaggattacc atcacagctt catatcctag 60
cgagggtcga gcgtctttca cgagtctcag tgagggtata gttgaagcgg ccgcgacacc 120
<210> 338
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 338
atcgattacc gactgtgtcc ggaagtgcca ctcctggaag ggcccatggt ggatgcttgt 60
gacgctctag catgggcacg gcacgaatta ccacaactgc aactaaagcg cccggatata 120
<210> 339
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 339
gagatccaag aagagtctca tcgtcaagag agcaactaca ttaccatata tcgcggtatg 60
aaagagaaat tatacgaatg ggtgcccatc gcacgcaacc tttgccggtc aatatctacc 120
<210> 340
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 340
cagaaatgct gtgttatcct tgcatcaact gacgatgcgt tgcaaatttg tcttgcgaaa 60
acatcccaag tcacaagggg accgccttct cggactgaga gcggatcgtt gcgtaaacga 120
<210> 341
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 341
tttgaatgat tatgtttctg gtcatcttgt tcctactatt aagtcagagc cggtaccaga 60
aacacaagag ggccctgtat atgtagtagt agcaaacagc tttaaagatg tggttcttga 120
<210> 342
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 342
gagtcgtatg gtcatcatag tgataacatg cgacatggag aagtaccagt atctcatatt 60
tcgtcaacag agttgcccgt ttcctcagca tcagtaccaa gctctaaaaa atgtaaatat 120
<210> 343
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 343
atacgtacac atcgatatgc acttgtcgtt caagacggga tcgttaccta tacaggactc 60
gagccagatc caggtgcagt gacagttagt ggagcacagg cagttcttgc ggcgctctaa 120
<210> 344
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 344
tgcgggagta tttccacggc accagatgtc gacgggcggt attggatgcg tatttggatt 60
catacgagag acccgacggt tgtacggacg gtgaggcacc atgtgatcaa tgcagcgtcc 120
<210> 345
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 345
ctgtttataa tcagtgccta tttcgggttt ttgtctggac tctaggctct agtgggagtc 60
aaaatgttct tcttgatcca tatactcaat atagggaaga tctgcgtaga tcttctaatg 120
<210> 346
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 346
acataaagtt tactctcgac ccagagggtg tattaacgtc ttcacctcaa ttgcgcttta 60
catataacaa gcttggagcc gacatccaat acactcttaa caatctctat ggagaaccct 120
<210> 347
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 347
aaacgagaaa tagaacagta atgactagag aggatggttc tttagaagtt gtttatattg 60
atgataattt tccatatgct atgccttcat caaatccaga atcaagtgtg ggagatgatg 120
<210> 348
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 348
gggtggacgg tgctatctac atctgttctg tgtcaaacgg ttttctacgg cagatttgcg 60
gcagacggac tatctacggc aaatctgcaa cagacggact atctatggca gatctgcaac 120
<210> 349
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 349
atcatttcca acaaaaagtg atgatggaac aataattggt ggtactattg aattggatgc 60
acaaaaagca agtgtagcat gtatgcatgg tgatatgaat tcatctgaat gggcattatt 120
<210> 350
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 350
tgagcgatgt cttctaagac ataaccggca ctctcaatat ctagccggaa tgcgaagctc 60
tgaccttcga aatctgtcac atcttcgaga tccaggagaa tatacatcga gagcgaaaca 120
<210> 351
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 351
taaacggatt ggtttcgttt gccccattca cgctcaacgg aacgaatatc tccgaatgct 60
ccagttatgt ctatctaggt cgggaaatca atatgatgaa cgacttagct ccagagctga 120
<210> 352
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 352
atgaaataaa agaggatgga tatgttgtac cacaggttct cccttccgaa atccgacatg 60
ccatttcgtc ggtgaaaaat cgaacagcac caggtccgga caggataaga tcagaacatc 120
<210> 353
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 353
ttgggctctt aacatagtag gcgcagaaaa aacacacgga aaccgccttg tgctcctttt 60
agcaacgcag cttcggactt taaaacccag gtggcataca cacagactag caaatgcggt 120
<210> 354
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 354
ccgctgtggc ttcttctggt aagccttatg ctccaaggaa ggctcgtgcg actcctgatc 60
gcattgatat aagtcgcctg atacatgccg atcgccgcaa acgctgctgc tctgccggaa 120
<210> 355
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 355
agcttctaga gagtgttcgt ggcaggccga aaatagtcgc tgcttttctg cagtccctca 60
catactccgc accattgact tgggtatttc actctcatga ggcaacggta ctgcagaaac 120
<210> 356
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 356
gttatgaagt tatataaagc actttatgac ggtcaagaga ttgcattcga cttatgtaaa 60
tcatgtcaac cttgctttga agagaagttt aacttctcaa taacgactaa aacaagcttt 120
<210> 357
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 357
aagagatata taccaaaaga atgcgaaact gttagaacta ttgacttact tcttgttgaa 60
gaccactaca tgctaaatga aagattacca atgacaacat acttcattcg aaactatata 120
<210> 358
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 358
atgcaagaga atagaaagca gacttgccgt ttatatgttg gacccagata tttcaaatgc 60
tttagctgcc aaatatattg cagttccact tatgttccct atacaccaaa tatctgtcaa 120
<210> 359
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 359
tccccaagaa agccagcggc cagctgccgt cgtccatcac tgggcgtcct cgttttaaaa 60
acaccacact gcagcgggat ggagcgccta acgccggaac gccgaccgag cattcatatc 120
<210> 360
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 360
aaaaagtagc agtcgtccac aggaaaagta cgcaacggct ctttttccaa gcaaaactca 60
cactgcaccc tcatggctca catcttcttc ctctctttac gttcgtcatg atgatggcga 120
<210> 361
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 361
gaaccgcgta caatacctgg agagaagccg cgagtcaacg gagacagcga tttgagatgc 60
tggagaaaca aagacgcaca gccgaagaca gcaggagaaa ggcgaagaga gaagagaaaa 120
<210> 362
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 362
gaatcagaaa ttgtgcagta taattgacag tgtgcataag tcatacatcc agatccccct 60
aagtttgtta atccaaacca ctctctttgt cataatgctt atgttttctc ttctggctcc 120
<210> 363
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 363
ttgtacattg gttgatctca gccttatagc ggctgctagt acgcatgaat ttttttcagg 60
cagaacaagc tttaaagcgt ttgggattgc tcataaacta ggcctattaa atgagattcc 120
<210> 364
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 364
gtgttcttta ggagacatac gtgcaggagt cttaccaaca acgctgtctg gacttcttcc 60
tcatcggaaa ctgtttccgc aagcaatacg tagaaaaatt tcaagtgaag taggagcgag 120
<210> 365
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 365
cgccaagact gcgagccaac aaagcgtgtt cctttgtctg tccatcctca tcgaacccag 60
cctcaaactc accaccagcc gcgtttacca ccaacaccgc tacatcagct tgagcagcac 120
<210> 366
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 366
actatgggta ttcatgggct gtgagcgttg ttctcttcat aacggagggt tatcttctct 60
atgataccct cgtgtatctc ttctattgga agatattggt tgggcggacg gaaatagctt 120
<210> 367
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 367
ggaagagtcg cctgtttcat caaataacaa ctccgtgata ttggatacca gtcgtttcgt 60
gctcaaagtg caaggctttg ccgagtacat ctttggagag gaaccttgtc tgaactttgc 120
<210> 368
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 368
cgcaatgact cggcgccgac tcggcgaccg gccaaaaggt ggtggatggg tgatgacagg 60
gttggtaggt cgtaaatccc ggtcaccttg gtagccacta taggtgggtc ttaagagaag 120
<210> 369
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 369
gccctggggt gcacgatttg cactgaccaa tgctggccac tgtatcaggc gggtttggct 60
gtgcggcctg gcaagtccgc ggcccaactg gtgggggagc tgggtagcct atacgggccc 120
<210> 370
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 370
gtgcagtcgc gtgtgagcta aagtgggaaa gtgagttttg gagatggact gaacagctgg 60
cctccaacta ctggattctg gaatacctct ggaaggtccc atttgatttc tggagaggcg 120
<210> 371
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 371
atgcttattt ctaaatccag cctttctttc cactgcacaa tcctctcatg aatggcagct 60
gcaaagtcag caactggcct aaaccagatt aaaagcaaaa gcaaagtcat gccactttgc 120
<210> 372
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 372
tttgccatct ttaacatctc aggaaattca aagaagaacc caaaaactat ttgttgaaag 60
tttagcaagg tttttggaag aaactacttg ggcaatagtt aattcaccag ttaacttata 120
<210> 373
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 373
tgatgagcaa cttttacacc ttgttccatt tttttatata aaaaattcat tctcttcatt 60
ttgtcttcgt ccccaccttt atcagggtga agttctttgc attttttcag ataagctttt 120
<210> 374
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 374
cctcggcctc ttatatatta taaaaaaaaa ggccacaggg aggagctgct aacccatgga 60
atgtagccaa accatgacct caggaaggaa agtgcatgac tgggcagcca gccagtggca 120
<210> 375
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 375
tcaaataaac acaagaggaa gtggaaactg gccaaaggag tggaaagcag ccagacagac 60
atgttttgcg agcctaggaa tcttggcctt gtccccagtt aaactggaca aaggccatgg 120
<210> 376
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 376
aagagctcag aggattttta tttttatttt agagcttttg ctggaatttt gtagaggtga 60
agacagtgta gacgggaaaa acaaaagtac cactgcttta cctgctgtaa aagactctgt 120
<210> 377
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 377
ttgacaaggg aataagagag aagaagctta aatatgaggc aggcttaacc tatattgaac 60
aagagttgca taagttcttc ctaaagggtg agatcccaca accctataaa ataacattta 120
<210> 378
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 378
gaaaaacagt atgaaaagga tccagatgag ttgaacaaga gaacattaac tgaccgagag 60
ggcgttgcag tatctatcca ggcaaaaatt gatgagttaa aaaggcaact ggcagatagg 120
<210> 379
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 379
caacctgttt ctttttattt gaaaagggtc ttcatggaaa tatgaatgaa gaagcaaaaa 60
ttcatcttga gacagttgaa tgggcactta aattcagaga gaaggaagaa aagtatgggg 120
<210> 380
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 380
aatggaagct atcggacctc gcttaggact cccattccca tggagaaact cctagatgag 60
gttcttgccc ccggtgggcc ttataactta accgtcggca gttgggtaag agaccacgtc 120
<210> 381
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 381
aagcgggcca tcgtagccgt gatacccaga cctgtgttca cgcagatgca ggtcagtgat 60
cacccagcac tccacgcaat ttcgcggtat acccgccgcc attggatcga gtggggccct 120
<210> 382
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 382
cactgtgcct ccacaggacg gcacgcaaac tcgccaccgc cctggccgag acggccagcg 60
aggcgtggca cgctgactac gtgtgcgcgc tgcgtggcgc accgagcggc cccttctacg 120
<210> 383
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 383
ttgccagaga atactgcaga gctattaata ttgaatatcg agatgatgta cctgctgttg 60
atattgttat ggagatggta ccagatctcg atcttgggaa aatagaaata cctgatgtca 120
<210> 384
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 384
gtgcccacca agtcttgctt ggactagtat agcccttaat caatgggtca cttttagcac 60
atataacatg ttgccaggac agacgaatga ccctgccagg atttttgaat gcagaaggga 120
<210> 385
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 385
attacgaagt tatgggaact acaactgcta ccagatggtt tcaagggagc acaacttatt 60
cattagagca tacttgtgaa catattcaag ttacttgtgg taatagggtt ctcacattcc 120
<210> 386
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 386
ggtcctggat gtgtatcttg gggaccaaat agaattgatg tttttgctag aggaagaaac 60
aataccttaa tccataaatg gtgggacgga tcacgttgga gtgattggga agacctaggt 120
<210> 387
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 387
attcatgatc tgtctggctt tggcagggct tctcttgcaa ctgtaatacc tatactatct 60
accatgggtt ttcaggtatg tcctatgcct acagctatac tttcaactca cactggtgaa 120
<210> 388
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 388
atagaggaac attgtttggg atacaccacg cttatagtat gggctacaag aaagtaggag 60
cggtttcaac tggaaatatg gcggtatctg tagcagctta tggacaaaga gcaggatttg 120
<210> 389
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 389
tcgacggtta cgagcaaatt acccctgtag cccgcctgaa aaagggacgt gttactccgg 60
atcaagcgaa ggcctatagc ctctacaagg atagcgtaaa gaatctcata cttgagagtt 120
<210> 390
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 390
tctcgacatt ttcctcgcac gctaccttga cgttgggaat tcatcttccc tcctccaggg 60
catctgagac ggaatccgaa ggagcaggat caagttacac gcctcctgtc ctatcgacca 120
<210> 391
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 391
cccacgaaaa caacatcgac acgccagcac taggtacgct ggttgcttgt ctcaagcgct 60
tactggagaa gcgtgggcaa ccattccaaa gaaccgcatc cgcgaaggtg cttctgccca 120
<210> 392
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 392
ggccgttgat cctgggccac ggaagcctaa catagaggac cgtaatgtgc gatttatacg 60
gagaatcttg aatcacaaga cctatccaag taagggacga gacttggaga tttttaccaa 120
<210> 393
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 393
actggttggt tgtgctgatg ttcccaagcc ctcaaaatgc ggaatgcata tcgatgctaa 60
catcagtcgt gaagatatac atgttgatcc gaattacaaa ccaagttcca ataccgacgg 120
<210> 394
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 394
tcccatttgg cggcacatta accagagact cgatgaagaa tagccaccac gaaacacccg 60
tgtgcaagca ccttctcgcc tgtgtgttgg ggagcctttg cccggcattg tttggtggtg 120
<210> 395
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 395
gacaaggctt ccattactaa aacgacctcg cagaaaccga aaaagtgcag ccgttcgatc 60
tataattcaa gaaacccaac tctgttctag tgacttgatc tggcccatct ttcttaaaga 120
<210> 396
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 396
agagttagag agactttgta cgataggatt gaaagcagtt atcctctttc ctgtaattga 60
tgctaataaa aaagaacaat ttggatccta tgcgtcccat ccttacaaca ttgtttgtaa 120
<210> 397
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 397
tgaaagtgtc cgtgtatatg ggggtatcgc tgtcatgcat gcggaaatgg gagcagatat 60
tgttgctcct agcgatatga tggatgggag agtgaagcat attcgagagc agatggatca 120
<210> 398
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 398
taaaggttta taccttccca ggtaacaaac caaccaactt tcgatctctt gtagatctgt 60
tctctaaacg aactttaaaa tctgtgtggc tgtcactcgg ctgcatgctt agtgcactca 120
<210> 399
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 399
gcttacggtt tcgtccgtgt tgcagccgat catcagcaca tctaggtttc gtccgggtgt 60
gaccgaaagg taagatggag agccttgtcc ctggtttcaa cgagaaaaca cacgtccaac 120
<210> 400
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 400
ccctatgtgt tcatcaaacg ttcggatgct cgaactgcac ctcatggtca tgttatggtt 60
gagctggtag cagaactcga aggcattcag tacggtcgta gtggtgagac acttggtgtc 120
<210> 401
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 401
gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 60
tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 120
<210> 402
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 402
cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 60
attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 120
<210> 403
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 403
tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc 60
tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa 120
<210> 404
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 404
gtaggagatc ctataccagg cgcagagacc ttctacacag atggatcatg caatagacag 60
tcaaaagagg gaaaagcagg atatgtaaca gatagaggaa aagacaaagt aaaagtatta 120
<210> 405
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 405
agtagaagca atgaatcacc acctaaagaa tcagataagt agaattagag aacaggcaaa 60
tacaatagaa acaatagtac tgatggcagt tcattgcatg aattttaaaa gaaggggagg 120
<210> 406
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 406
tggatgggct gtgaactatg gccaactaaa tggaagctgc agaaactaca actgccccag 60
aaagacatat ggacagtcaa tgacatccaa aagctagtgg gagtcttaaa ttgggcggca 120
<210> 407
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 407
tttttcaatg gtataatatt aggacctgac ggcaatgtct taatcccaga gatgcaatca 60
tccctcctcc agcaacatat ggagttgttg gtatcctcgg ttatccccct tatgcacccc 120
<210> 408
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 408
ctgggaatca taggatggtc gggttagtca aagtagttat tggactggct ttgtcaggag 60
ctccagtccc tgagggcatg aactgggtat acaagttgag gagaaccctt atcttccagt 120
<210> 409
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 409
aaccacgatt acaccatttg gatgcccgag aatccgagac tagggatgtc ttgtgacatt 60
tttaccaata gtagagggaa gagagcatcc aaagggagtg agacttgcgg ctttgtagat 120
<210> 410
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 410
cgcctctata cgcagttgaa gcaaaaagga ttcagaaatt ggagcacact attaacaatt 60
acatacagtt caagagcaaa caccgtattg aaccagtatg tctattggtg cacggcagcc 120
<210> 411
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 411
gagcgtatgg tggttctacc attaattaca ccaccattaa ttattataga gattcagcta 60
gtaacgcggc ttcgaaacag gacttctctc aggacccctc caagttcact gagcccatca 120
<210> 412
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 412
agttgatgga taagtcgtct agagtcagat acagcatcga ccaaatcact accatgatca 60
tcaacgagag aaatagacga tccaacatag ggaactgtat ggaagctcta tttcaaggtc 120
<210> 413
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 413
tactgggggg atgtcaagca ggacttggtg tcatactgtg ggccttggaa gttggatgca 60
gcttgggatg gactcagcga ggtacagctt ttggccgtac ctcccggaga gagggccaga 120
<210> 414
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 414
ttgaagaaga gaatgaccac aaagatcatc atgagcacat caatggcagt gctggtagtc 60
atgatcttgg gaggattttc aatgagtgac ctggccaagc ttgtgatcct gatgggtgct 120
<210> 415
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 415
tccctgaaag ggaaaggtag tgtgaagaag aacctgccat ttgtcatggc cctgggattg 60
acagctgtga gggtagtaga ccctattaat gtggtaggac tactgttact cacaaggagt 120
<210> 416
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 416
atgtacctca gctgaagcca gtttcagaat tgacttttct taagagatct ttcaatttgg 60
tggaggatag aatcaggcct gcaatttcgg aaaaaacaat ttggtctttg atagcatggc 120
<210> 417
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 417
ttgatgcttg tcctctggat tacacaattt tgtgtcgaat gtattggggt ccagctatta 60
gttattttca tttgaatcca gggttccata caggtgttgc tattggcata gatcctgaca 120
<210> 418
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 418
tgaattcaaa tgccatgtta tccactgaat caatgatgag cagaattgca gctggagact 60
tggagtcatc agtggatgat cctagatcag aggaggacaa aagatttgag agtcacatag 120
<210> 419
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 419
ctgttctgac tactgcctct cccttatcgt caatcttctc gaggattggg gaccctgcgc 60
tgaacatgga gaacatcaca tcaggattcc taggacccct tctcgtgtta caggcggggt 120
<210> 420
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 420
tgtctttggg tatacattta aaccctaaca aaacaaagag atggggttac tctctaaatt 60
ttatgggtta tgtcattgga tgttatgggt ccttgccaca agaacacatc atacaaaaaa 120
<210> 421
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 421
acaaggcctt tctgtgtaaa caatacctga acctttaccc cgttgcccgg caacggccag 60
gtctgtgcca agtgtttgct gacgcaaccc ccactggctg gggcttggtc atgggccatc 120
<210> 422
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 422
aacatcggtg atccagtaac atcatcaatt gctgatctca agagaatgat tctcgcatca 60
ctaatgcctg aagagaccct ccatcaagta atgacacaac aaccggggga ctcttcattc 120
<210> 423
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 423
gttcacattt cttttgtgtg aaagcgatga ggatgtagta ccggacagat tcgacaacat 60
ccaggcaaaa cacttgtgtg ttctggcaga tttgtactgt caaccaggga cctgcccacc 120
<210> 424
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 424
atggaaccaa tcgcagatag ggctgccagt gaaccgatca catgatgtca cccagacatc 60
aggcataccc actagtgtga aatagacatc agaattaaga aaaacgtagg gtccaagtgg 120
<210> 425
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 425
ggcgttcacc gccatgctgg cgagggcgca gaatttcagg caggcgatga acggcttttt 60
caagagtatg gggcaaacct ttattcaaga aatggttacg aaacctttga cgggcttgat 120
<210> 426
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 426
tgaatagcct agacgttatt gccctactca aacaagacgg ttggtataaa gttgcacaat 60
ccgggagcca ttcgcaatat aagcacccaa caaaaaaagg ccgtgtaacc gtgccgcacc 120
<210> 427
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 427
tgccgagatt gtcagatgag gattggcgca aagtcgagct ggattaccgg cgcggggttt 60
tgagcattgc cgaaatcggg cgcaaataca atgtttcggc gcaacacgtc ggcagggttg 120
<210> 428
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 428
gctcaaaatt ggcattgttg caggctgcca gttgtacata gcagggggaa atggaaccaa 60
cggctcttcg agttccggca ccaacggtaa cggcaacggc aaactgctcg ggggcggggg 120
<210> 429
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 429
gcaggaagta aaaaaagacg ctgcgcgtgt acaggtcgca cccctgttgc gcgcagcaga 60
cgcagacgtg cgcacacatg ctgcacgcaa aaaaatggta caggcgttgc gcctacacat 120
<210> 430
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 430
tgtgctgcgt gtgctgctcc tgcagaacac cggtttgagc taaacgcgga ggcgtggcac 60
tttctcaaca cggtcaaaga atgcactcca aggcacgcgc gcgcgttggt gttgtcccaa 120
<210> 431
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 431
gcagaagatg aacaaggcat tgatgagcct agtatttata aggctgaaaa acaaaatcat 60
ccagcagcca gtgcgcaacg ctatctgaaa cgcgtggttt atggtaatcg ccaagcggcg 120
<210> 432
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 432
atattgctga tcgtttttcg aaaaagatct tactgcccgt agggttgatc ggtgtgggcc 60
tctgtggcgc atacttagcc acgcttccac cgtttaccgg ttatctcatc gcctttggtg 120
<210> 433
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 433
gttggctacc gcgcagaggg gactatcaac atcgacttgc ttggttatag ctgggacgaa 60
accaacggcg gcaagaatcc aagcctgaca gaaattggcg cgaccaatag ctggaaaaaa 120
<210> 434
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 434
catatgcata ttcaattatt gatagtacca atggcataaa atggggagac ccgatccctt 60
ggatgtctga cattcctatt agcgctgggg ataattctta tacaattaat agcccgcaaa 120
<210> 435
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 435
caatataatg ggcactccat tttggttcga atttgaatgt aattcagatt ccgctactcc 60
atattttaga tttgaattgc cggagttgat gaaaaaactt cacgaaatta gacaaagtgg 120
<210> 436
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 436
taaagattat tgcgataaat tgatgggtaa gcatttaacg ggacgtgatg aaataatgag 60
gtcgatatat tttgcggata aagtattata cgacaagtac catcgcaagt taccagaatg 120
<210> 437
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 437
cgccggcctt taccgcaacc catgagaacg agaacgatcc gtcggcagcc gacatggcgg 60
ccgccctcaa tctgatcaac caccgtcaag tctcggcgtt gctggtaaac ccgcagaaat 120
<210> 438
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 438
cgtcctcaca gttcgtctcc ggcctgttgt tgtgcgcagc gtcgttcacc caaggagtga 60
ccgtgcagca caccggttcg ccggtaccgt cagctccgca tatagcgatg acggttatga 120
<210> 439
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 439
tcaagatcaa cgacctgatg ctgccgttag tcaaaggggt gggctccgag caggcctacg 60
cgaaactcac tgagagcctg cagaccctcg gtggttcggg cttcctccaa gactacccga 120
<210> 440
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 440
gtaagcttca cactgaagct gagcaacgcc gacgaaacgc cattaaaaga ggtttcgaag 60
gcttactaga gcttgttcat ccaatgaagg gtgattcact ttcttcgtca gtacggatga 120
<210> 441
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 441
agtgttctgc tgcagcactt gatgtgctgg ctagtgtttt tcatacagat tttcttccta 60
ttctgcttcc aattacaaag gagttattat tcgctcctca atgggaacta aaagagtctg 120
<210> 442
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 442
tgctgctgct tcgtctcctc aatctcattt atgttatatt gttgctccga aatctcctga 60
ttcacgtgtt cgttggttaa gtgaattgac atcaatgatc ctagaagcta gacgtttatc 120
<210> 443
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 443
cgggtttcgt gtcactgttc caatcgacta ttcgctgctc cctgattggc ggtcttatcg 60
cgtcatacat caaccaggcg tccactcggc tacaagcata acaataagag acccctattt 120
<210> 444
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 444
aaacctggag tcactagacg accgtttcgt ccaattgtgg gactcctcag cagtgaatat 60
ccacaatccc tcctcgcgag attcgaacac aggacctatc agtctcacgc acgagcgctt 120
<210> 445
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 445
atttttcata gtagaaagca cgagttaatt gaagctagac caccatggaa aatctggaaa 60
cactggaggg ccgtttcgtc ctattatggg actcctcagc agtgcgcgaa cctgccctcg 120
<210> 446
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 446
acgatgcgaa gaacgatgag aagaacgatg agaagaacga tataaacaac gctagtagta 60
ataacaacaa cagagataac attcgtagta acattcgcgg caatgagaac tccaacgagc 120
<210> 447
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 447
attgggtgcc gacccaaagt tggctagtca attggaaaaa caaattaccg ctccatttta 60
tcaacagcat aatttacgac ttggcaattt tgatcgaaga ggagtgcgat gaaaaagaag 120
<210> 448
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 448
gggttaggcg aaatgatggc cgatcaactg tggaatacaa caatgaatcc acgagtgagg 60
aaactaatac gggtaacagt gaatgatgct ataaaagcga ataatatgat aaattccctc 120
<210> 449
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 449
ttatagtacc tccaagtatg aagaacttgt taacgaacat acgatacgaa aaaatacaaa 60
cagaagaaat tccttgtatg catatcctac gcaaaataga atatccgatc aaatggaaaa 120
<210> 450
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 450
atacggctca gaccaacatg atcaacgtaa taatagtatg gatgctgtga atagtgtaaa 60
tcatgtgaat cgtatggatg gcgtaaatca tgtgaatcgt atggatggcg taaatcatgt 120
<210> 451
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 451
gattgtgcac ttatcgtaca aaaactcaaa aataaattcc cacatattga aactatacct 60
agtggatcca tatgttatgc tactacaaat agacaaacgg ctcttaataa aatatgtaca 120
<210> 452
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 452
atcgatacag gtgttgagat aacaacagga tgttttggtg atagcgattt gagtgcttca 60
tgtattgatt ataattcaga aggaaaaggc tgtgttaacg gagctgcagc aatgtcagtc 120
<210> 453
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 453
aggcaatgag aaaatcacca gagcacgcta tgatggctaa taaccaaatc acttacggag 60
ctcctgttgc aattactatg acgtgcaaaa aggatatgat gagatgggct gaatttgact 120
<210> 454
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 454
ctttgcacta ttggacatga aaacgctgaa gaagctaaag tcaaggaaat ccttactgct 60
gctggtgctg aaatcgatga agctaaaatc aaacaagttt ttgatgccat gaacggtaag 120
<210> 455
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 455
aatgcggcta tcgcagagat gcaagaggag ctgcatctgt ggaaggagag gacaaggaca 60
acggcagagg agctagccgc ggcacgccac gcggcaagcc agctccagga agacgtgaaa 120
<210> 456
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 456
ctacgaggtg ctgtcggacg acaagaagaa ggccgcgtac gacgagtcgg gcataatccc 60
tggcgaggcc ccaaacttgg aggagatgac ggccaacgag ctcttcgagt actaccacca 120
<210> 457
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 457
gcatgtctca gcacgcgcct cagtcggtgc atactcggtc gcgcatcctt ggcagagcac 60
atgtcgccct cgtcactctc tccttcgcgt ggcagtatta catcgtgatg ttcgtggctc 120
<210> 458
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 458
tcgagctcgc ggggacctgg tcgggaacgt cggggtcggc tgtgcagtcc gcactgtcga 60
actacctcac ccaggcctcg gatgtccgtc gggaagagct cgatatgtcg cacctgctca 120
<210> 459
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 459
gtcgaaccga tcgaagtctc tcccggaatc accgtccacc cgctgtggca gggcgccaac 60
ggcgccaaag ccgttgtcac caccattgct gcaggggctg tctgggacgg tgaagacctc 120
<210> 460
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 460
cgttccgggg gcactatcgc cacgtcttgt ccgataccga actggcaggc gtctcattgc 60
ccacaaacag ccatctactg ctgctgtggg gcgcggccaa ccgggacgag gcggtgttcg 120
<210> 461
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 461
tctctctcct tcaccacact tcaccaccaa actccagatg tctcatgtgt cagccagtgt 60
ggcgcttcat tgcagcattg acagacgttt ctcagtgctg ttatcaatgc gccgcactca 120
<210> 462
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 462
aggactctgc ggaaacatct gatgcaaagc ttagcagatt ccagcgaata cttaatttct 60
tcgcgcatag aagtcgttca atgtggacgt tacggtcaca caccttagta ggagaggaaa 120
<210> 463
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 463
ttcgcattac tccgcaccat gagcatctga tgcacgtggt cgtggtccac gctctggcct 60
cgcactacca gccagaccat ccgcggacgc ggcagcacag tagctgcgcg cgaaggaaac 120
<210> 464
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 464
aaagactttt aagagacctt gaatatggat ttagagcaac tttagccagc ataggagaat 60
cagatcctgt aaatgctata gctactcaag ttagaagctt agctagcaca gcaagggaaa 120
<210> 465
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 465
atgtgaaaca aactctaaca actctttagg aaattctaaa gaagcatcaa atgcatgttt 60
tttcttagga ggggttgcct gagaacagct aggctcctct tctggtgcac ttctttttgg 120
<210> 466
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 466
gtgacctctt attaacatgc tttttttcta agttaacctc aacacaacca tcaaggtagt 60
ctcttaagtt atcaagatta tttaccccat taccagaagg tagttgcttt tcaataccta 120
<210> 467
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 467
ttccgggcac ggcgccaact tcctgggcct ggtgccaata ccaacacagc tgctgagctt 60
ccggaataca atactggtgc cctttgtaag tgttttacag gtaagtaagg cctacaacag 120
<210> 468
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 468
cttataaaac ataaaagtac ccctataata aaaacatgct tacctggtta agccaacccc 60
ttgcatggtt gtaagaaata taatcttttt ccagtaaaaa aatgtttctg cactaatttc 120
<210> 469
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 469
ttgtatacat gggtgaaaat cactaacagg cctgcaccat attaacataa gtaaaataca 60
cattccactt tgtaaaactc tttttaccat tagttctgga gttttatcca gactttcttt 120
<210> 470
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 470
tattccatgt ggaagctgaa gtacctgaac gttaagctaa cctctatggt tggtgcatct 60
gctgtcaatg gtactgtcct cagagtttca cttaacccca catctacacc atcgtccact 120
<210> 471
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 471
ttaggaaagg tgtgaaactt cttactgagg aagagtatcg agaactctta gaaaaaggtc 60
tagatcgtga aacattcctt gatctcatag accgcattat tggtgagagg tctggctatc 120
<210> 472
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 472
ccacgctcca gggcggctgg ctatggctat gatagtgaca acactgaata cttggatgcc 60
ccagactttg ctgaccagct cagagaagac atagagacag acacagacat cgagagtaca 120
<210> 473
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 473
tgttatgatg tctgcaccac ctgctgagta taaattacag caaggtacat tcttatgtgc 60
gaatgagtac actggtaact atcagtgtgg tcattacact catataactg ctaaggagac 120
<210> 474
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 474
tcacttactg tcttgtttga tggtagagtg gaaggacagg tagacctttt tagaaacgcc 60
cgtaatggtg ttttaataac agaaggttca gtcaaaggtc taacaccttc aaagggacca 120
<210> 475
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 475
aaatttggct tggcctctta ttgttacagc tctaagagcc aactcagctg ttaaactaca 60
gaataatgaa ctgagtccag tagcactacg acagatgtcc tgtgcggctg gtaccacaca 120
<210> 476
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 476
aaatttcctg aagtagtatt tgaaagtaca cttaggatta ctagaactct gtcaactcag 60
tactgccggt tcggtagttg tgagtatgca caagagggtg tttgtattac cacaaatggc 120
<210> 477
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 477
cgtcctccac caggtgaaca gtttaagcac ctcgtgcctc ttatgcataa gggggctgcg 60
tggcctattg ttagacgacg tatagtgcaa atgttgtcag acactttaga caaattgtct 120
<210> 478
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 478
tatgttgcac cacaggtgac ataccaaaac atttctacta acctccctcc tcctcttctc 60
ggcaattcca ccgggattga cttccaagat gagttggatg agtttttcaa aaatgttagc 120
<210> 479
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 479
taccaccatc cttggcatcg gaacagtcct tgaccaggct gagaccgcag gtgttaggct 60
ggtggttcta gccacagcca cgccccccgg tacggtgaca actccccacg ctaacataga 120
<210> 480
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 480
ctgggcccgt ccagactaca accctcctgt gatcgagaca tggaagaggc cggactatga 60
accacccact gtcctaggct gcgccctacc ccccacacct caggcaccag tgcccccacc 120
<210> 481
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 481
tgtaaaacac cgcggtgcac tcactcacaa cctgcgagca cacgtcgatg tgatcgtaat 60
ggcagctacg gtctgctcgg ctctgtacgt gggagatgtg tgtggggccg tgatgatcgt 120
<210> 482
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 482
gtatgatttc cttgtttgcg gttcgtattt tggacgtaaa acatcttcca cctcagatgt 60
tcgaaacttc gtacgtgtat tctgaattta tcgaagtgaa aaccggaatt cctttgagag 120
<210> 483
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 483
tctgaatata tcagaaggcg gaatcctact cgaagtaacc gatccagaat tgattcaatc 60
ttttttgcac aaaccagttt ttactgcgga cattactttt aagatgcaag cccctctgcg 120
<210> 484
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 484
tgttatggat agttgccgtt ttttccgtag gttatttttg ttattctacg tgggatgctt 60
ctgtaaaata tcacttcact gcacatcact gggactttaa cgcagaaaac gtaagtagaa 120
<210> 485
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 485
agtggttgaa gagaccaatg ccatgagcca acgggcgact caagttgacc atttagcgtc 60
gaatcaaaga gaagagaccg aacaagtggc tacagcgatg accgagatga caacgaccgc 120
<210> 486
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 486
agcctctact acttatctga tctcaacggg tctacccaac tgcagcgttt tgagctagaa 60
acgggtcaat caacgaggct tgcagagtgg cgttcggcag agtggctgtt ctcgctctat 120
<210> 487
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 487
cgctttccct tgcatggagc gcggctgatt gaagcctcgg caggcaccgg aaaaacctat 60
accatcgcag ggctgtatct gcgtctggtg cttggacatg gctgcgcgga aactcgccac 120
<210> 488
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 488
cttcaaattt aaatgcagtt atgttttggt tagaacgaca ctcaaatgac gttgctgatg 60
caaataaaat ttataagcgt agactagatt tatttaccat agtggcatct acaataaata 120
<210> 489
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 489
gcatggccag ttatgaatgg aggtgccgtt tcgttgcatt ttgctggtgt tacattatct 60
acgcaattca cagattttgt atcattaaat tcgttacggt ttagatttag tttgacagta 120
<210> 490
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 490
tgttaactac taattctaca ttcaaagtat ttgactcaga agatttcatt atagagtatg 60
gaacaactga cgatgaagta tatatacaga gagcatttat gtcactgtct agtcaaaagt 120
<210> 491
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 491
acggctgaaa gaccttacgg aaccgatgtg tcaatacaat ccatgtgcgg atcatccatt 60
tattcgtctg ccgttaatgc gtaccgtggg tttggcgatt catattttgc gttcatgcaa 120
<210> 492
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 492
agatataatc gaacaggtag ttcatagacc gaatataggt atgactggtg gaaatattag 60
agctactctt ggtaggtggt caggtaataa aggggttgta acctgtatgt caggtatgga 120
<210> 493
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 493
tcgctcgtaa agataaagaa acgctcgaga gcatttctaa atcaccactt gcagctcaat 60
gtggtagtaa atttctcgat atgctcgatg gaattagatc aaaacaaaaa attgaagatg 120
<210> 494
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 494
ggatatggtt agtgggaaag aagatatgaa aaatacagat aaagcaaatt acgcatctgg 60
tcatgtcttc aatgctatag cacattatag atttgattac acatatgata ttgtaggatg 120
<210> 495
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 495
ttcaattata aaacgtacat taagctattt cggtctaagg acacacgact atgatataaa 60
aggatcctca tcgactgtat caaaaataat caaacagtat tctgtatata ctcctgggat 120
<210> 496
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 496
ttgttcttca ttctcagttg atgtctcatg cagaaagatt agataggaaa attaagtcag 60
ataaaaaaca tggtagacaa ttctcttatg atgatggaga tatggctttc gtacatccag 120
<210> 497
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 497
gtgatgctcg aaaatatcgt ccgtcatgtg gaggaggggc ttgcgccatt tcaggccgct 60
attgtcggca gccaggaagt gacaggcaca atcatttcca tgtcgctgtc actggtcgcc 120
<210> 498
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 498
tgatgcgctt tgtgaaacgt tccgcctttt atctgcttgt ggcgctcatc attctggttg 60
cggtgtttcc gttctattat gcgatcatca caagctttaa atcgggaacc gcgctgtttc 120
<210> 499
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 499
tccgcttttg cgcgtatgcc caagccggaa atgcggcata tggacctgcg cgaagcattg 60
cgtgaagcct cgttcctgat tgaagtcagc cgcagcgaca ttcatttcga caatgatttc 120
<210> 500
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 500
ggccgacgag tggtcagccc gcgaacagcg ctgggcgacg ttgcgtgacg gctctgagta 60
cattcgcaac agcaccgaag gccatgccct cgacctgctc gaaagcggct tccccgactt 120
<210> 501
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 501
ttcaacgaca ccgggtacag cccgctggac gtcctcgacg acttcgccgg gttctacttc 60
gacaccgcgc tttcggcgag cgcggccacg ctgccgaccc tgctcgcgtt cgccgaaccc 120
<210> 502
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 502
cacctacctt ttcctgccgc ttcaattcat cggcctgctc atcgcgggct acctgtgggc 60
cagccacgag ttgagcctgg ccgacaaact cggcttggcc gtcaccctcg gcttcgtctc 120
<210> 503
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 503
gattcttcat cgctaactca cttatgtccg atgccggcgg aaatcgcgag atcgacaaac 60
tgcaacggtg cgcagatgcc gtcgcctcga ggggtttcgg aatcaaacgg cagatgctgc 120
<210> 504
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 504
tggaggcgtt gaaggggaat cgcaaagggc aatacagcct tcggatcaac gagcagtgga 60
gaatctgttt cgtctggacc gatgcgggac ccgaacaggt ggcgatcgtg gactaccact 120
<210> 505
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 505
ggtgacctcg atctgcccct caggggaaat ttttgatcgg gcgaccggtg acagcctcat 60
cgatggcatt tcggttgaac gggggttgta tcttcgcgta ggcgctgccc gatctcgagc 120
<210> 506
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 506
cgccgcgaaa acgatcaaat acctgctgga cgaactctcg tccggattca aatcgcttga 60
taccgacgtg gtttcgctga ccaaaacctg gcagggctac cagggcgggc tgttcgcgca 120
<210> 507
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 507
tcgtctcaga agtaccgctg cactacaaac gaccggatgt catcgtttac cgctgtatcg 60
aagaaccccg tgggcgctgg aagttgaagc cgaccatcgc ggaaaccatg ctggtggtcg 120
<210> 508
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 508
tgtcactgcg gacatgatcg acaaacgggc cgagtacgcg cgattcggca tcgaaaacta 60
ctggatcgtg cgcatggccc gcgacgacgg tcccgcgatc tcggtcgaga tgctctactt 120
<210> 509
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 509
tcggatttcg actccaaccg cagcggaggc atcgtcgcgg tgaacctgcg cgacaacgac 60
gaactggtcg gtgctgtgct gtgctcggcg gacgacgacc tgctgctggt gtcggcgcag 120
<210> 510
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 510
tcatcgccgg catcttcgcc cagacctgtg tgttcggcgc gaccttcacc gggctgagcc 60
tggtggagga catgcagaag ggcatcatcg accgattccg gtcgctgccg atggagccgg 120
<210> 511
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 511
gttcgagacc tattactccg agcatccgaa cgaggcgttc gcggcgcagg tgtggaccaa 60
taacgcctgg gtgtcggcga tcgccctgtt caccggggtg ctgatcctgc ccgcgctgta 120
<210> 512
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 512
ccgaccggct cggcgtcgcg gtgagcatcc tgggccggat gcgcagcctg cccggcggag 60
acgccgagct ggcctcgcgc cccggccagg gaacgacggt gacgctgtcc tggcacgacc 120
<210> 513
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 513
cgcgccgggc tgggagccgc gcgaactgcc cgccgccgac gaggaccggc tgtggacgct 60
gctgcgccgg atggcccgga tgtcgtatcg caaggcgccg gtaccggtca cgctggcaac 120
<210> 514
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 514
ggtggacgag gagaacgtcg gtgaaccgac cgggttggct tgctacgacg gcgacggcgc 60
ggtccggttg tacgccttcg atcccggcac cggtgccatg ctgctcgaac gggcgcggcc 120
<210> 515
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 515
accgccatcg tgacctcggt caccaagggg ctgatcgaac ccctgctggc gattctgggc 60
accaatggcg aactgggcct gggctttcag atcatcggcg acaaacccgc caccttcgtg 120
<210> 516
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 516
ggggccgatc atcagcgcgg ccatcaattt cgtcatggtg gcggcggtgc tgtacttcgt 60
gctcatcctg ccgatgaacc acctgaagaa gcggttcgcg accccgaccg aggtcaaggc 120
<210> 517
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 517
tgtcctcgcg gtaccggagc gactgtccgc catggtggac gcgctcggtt cgccgctgaa 60
tattgttgta cccgaacaga tcacggccaa cgtggcggag tatcgcgcgg tgtacgatcg 120
<210> 518
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 518
agtcaaacgg acctgtatgt gcatacattt gataaagtgg cttccatcca gaaccttcgt 60
gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat 120
<210> 519
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 519
atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt 60
ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc 120
<210> 520
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 520
tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct 60
tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta 120
<210> 521
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 521
ctggttgaac tgcttttcaa tgatcctgag gtaaccaaga ttcaaacgga cccgtcaccg 60
agcaacatgc gcgcgattcg atgctacgag aaggcggggt ttgaaaggca agaaaccata 120
<210> 522
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 522
ctcggaagcg gcgacgggtg gtgggaagac gaaaccgatc caggagtacg cggaatagac 60
cagtcattgg caaatccctc gcaactggac aagggcctgg gaaccaagct agttcgcgcg 120
<210> 523
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 523
ccaacactcg ctgacgtgca gaaacagtac ctgccaagcg ctctagcgca agagtccgtc 60
actccctaca ttgcaatgct gaatggagag ccgattgggt atgcccagtc gtacgtagcg 120
<210> 524
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 524
ctgcaaaagc tggcgctggg ggatgcttta ggccaggttc agcgcgagaa acttagccac 60
tggttgcgcg gcaataccac cggtgcggcc agcattcggg ccgggctgcc atcgggatgg 120
<210> 525
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 525
gcgtttgccc gcagtattgg cgatgataaa ttccggctcg accgcaatga accttcgcta 60
aataccgcca ttcccggcga tcttcgggat accagcactc cacgagctat ggccttaagc 120
<210> 526
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 526
atgatcaata aacggctgag tattgctctg gcgctggcgg ccatgatagg tacgcctgtg 60
gcgatggccc tcgagagcca gaagccgggg agcgattctg ctaatcatat tcagcaccag 120
<210> 527
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 527
cggaaaccgg gcgatatgcg tctgtatgca aacagcagta tcggcctgtt tggtgctctg 60
accgccaacg cggcggggat gccgtatgag cagttgctga ccgcgcggat cctggcaccg 120
<210> 528
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 528
aaggggatca cattgctgga tctggctacc tataccgcag gcggactgcc gttacaggtg 60
ccggatgcgg taaaaagccg tgcggatctg ctgaatttct atcagcagtg gcagccgtcc 120
<210> 529
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 529
atgaaaaaat cgttatctgc aacactgatt tccgccctgc tggcgttttc cgccccgggg 60
ttttctgccg ctgataatgt cgcggctgtg gtggacagca ccattaaacc gctgatggca 120
<210> 530
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 530
ttgatggaaa aatatggtaa agcaaagctg gttgtttcag gtcatagcga aaccggagac 60
gcgacacact tgaagcgtac ctgggagcag gctgttaaag gacttaaaga aagtaaaaag 120
<210> 531
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 531
atgaaaaaat tatttgtttt atgtgtcttt gtcttttgta gtattactgt cgcagctgag 60
actttgccta atttgagagt tgaaaagctt gaagaaggtg tttatgttca tacatcgtat 120
<210> 532
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 532
aatgaacttc tgaaaaaaga cggtaaggtt caagccaaaa actcatttga cggggttagt 60
tattggctgg cgaaagataa aatagaagtg ttttatcctg gccctggcca cactcaagac 120
<210> 533
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 533
tggagcgcgg ctatgaaatc aaaggcacta tttcatcaca tttccatagc gacagcacag 60
gaggaataga gtggcttaat tctcaatcta ttcccacgta tgcatctgaa ttaacaaatg 120
<210> 534
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 534
aacttttgaa aaaatccggt aaggtacaag ctaaatattc atttagcgaa gttagctatt 60
ggctagttaa aaataaaatt gaagttttct accctggccc aggtcacact caagataacc 120
<210> 535
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 535
tagtggtttg gttgcctgaa agtaaaattt tattcggtgg ttgctttatt aaacctcacg 60
gtcttggcaa tttaggtgac gcaaatttag aagcttggcc aaagtccgcc aaaatattaa 120
<210> 536
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 536
ttagtgtcca aatatggtaa ggcaaaactg gttgttccaa gtcacagtga agttggagat 60
gcatcactct tgaaacgtac attagaacag gctgttaaag gattaaacga aagtaaaaag 120
<210> 537
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 537
atgaaaaagt tatcagtatt ctttatgttt ttgttttgta gcattgctgc ctcaggagag 60
gctttgccag atttaaaaat tgagaagctt gacgaaggcg tttatgttca tacttcgttt 120
<210> 538
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 538
ggcggctcca tcggtgtgta cgcgatggat accggctcag gcgcaactgt aagttaccgc 60
gctgaggagc gcttcccact gtgcagctca ttcaagggct ttcttgctgc cgctgtgctg 120
<210> 539
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 539
atgtcactgt atcgccgtct agttctgctg tcttgtctct catggccgct ggctggcttt 60
tctgccaccg cgctgaccaa cctcgtcgcg gaaccattcg ctaaactcga acaggacttt 120
<210> 540
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 540
gtgtatggct cggcaaatga ctatgccgtc gtctggccca ctgggcgcgc acctattgtg 60
ttggccgtct acacccgggc gcctaacaag gatgacaagt acagcgaggc cgtcatcgcc 120
<210> 541
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 541
accgacatcg cttttggtgg ctgcctgatc aaggacagca aggccaagtc gctcggcaat 60
ctcggtgatg ccgacactga gcactacgcc gcgtcagcgc gcgcgtttgg tgcggcgttc 120
<210> 542
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 542
gcgcatcagg acaagatggg cggtatggac gcgctgcatg cggcggggat tgcgacttat 60
gccaatgcgt tgtcgaacca gcttgccccg caaaagggga tggttgcggc gcaacacagc 120
<210> 543
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 543
actggcgacc aacggtttgg cgatctggtt ttccgccagc tcgcaccgaa tgtctggcag 60
cacacttcct atctcgacat gccgggtttc ggggcagtcg cttccaacgg tttgatcgtc 120
<210> 544
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 544
cggggtgcgc gcgggattgt cgccctgctt ggcccgaata acaaagcaga gcgcattgtg 60
gtgatttatc tgcgggatac cccggcgagc atggccgagc gaaatcagca aatcgccggg 120
<210> 545
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 545
agcgcccgtt cgcaacggca gctgctgcag tggatggtgg acgatcgggt cgccggaccg 60
ttgatccgct ccgtgctgcc ggcgggctgg tttatcgccg ataagaccgg agctggcgag 120
<210> 546
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 546
atgcgttata ttcgcctgtg tattatctcc ctgttagcca ccctgccgct ggcggtacac 60
gccagcccgc agccgcttga gcaaattaaa ctaagcgaaa gccagctgtc gggccgcgta 120
<210> 547
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 547
atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 60
gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 120
<210> 548
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 548
gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 60
cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 120
<210> 549
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 549
tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 60
ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 120
<210> 550
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 550
atgttaaaag ttattagtag tttattggtc tacatgaccg cgtctgtcat ggctgtcgca 60
agtccgttag cccattccgg ggagccgagt ggtgagtatc cgacagtcaa cgaaattccg 120
<210> 551
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 551
cttttgattg atacagcgtg gggtgcgaaa aacacagcgg cacttctcgc ggagattgaa 60
aagcaaattg gacttcccgt aacgcgtgca gtctccacgc actttcatga cgaccgcgtc 120
<210> 552
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 552
ggcggcgttg atgtccttcg ggcggctggg gtggcaacgt acgcatcacc gtcgacacgc 60
cggctagccg aggcagcggg gaacgagatt cccacgcatt ctctagaagg actctcatcg 120
<210> 553
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 553
gaggcgtcac gcgaatccgc gggtaatgtt gccgatgcca atttggcaga atggcctgct 60
accattaaac gaattcaaca gcggtatccg gaagcagagg tcgtcatccc cggccacggt 120
<210> 554
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 554
ggagttgatg tcctccgggc ggctggagtg gcaacgtaca cctcaccctt gacacgccag 60
ctggccgaag cggcgggaaa cgaggtgcct gcgcactctc taaaagcgct ctcctctagt 120
<210> 555
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 555
ggagatgtgg tgcgcttcgg tcccgtagag gttttctatc ctggtgctgc gcattcgggc 60
gacaatcttg tggtatacgt gccggccgtg cgcgtactgt ttggtggctg tgcagttcat 120
<210> 556
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 556
cagaaacgct cgtacattgg attcgtaaaa atggttatga gctactgggg agtgtttcta 60
ctcattggca tgaggataga accgcaggaa ttaaatggct taatgaccaa tcaatttcta 120
<210> 557
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 557
gaggtggtca tacaatagac aacgtagtgg tgtggttgcc aaagtcgaaa atcttatttg 60
gcggctgttt tgtgcgtagc cttgattccg aggggttagg ctacactggt gaagcccata 120
<210> 558
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 558
cgtatgccac gacttcaacc aaccatctct tgaaagaaaa taaaaaagag ccagcgaaat 60
acaccttgaa aggaaatgag tccacattgg ttgacggcct tatcgaagta ttttatccag 120
<210> 559
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 559
atgaaccaga aaaaccctaa agacacgcaa aattttatta cttctaaaaa gcatgtaaaa 60
gaaatattga atcacacgaa tatcagtaaa caagacaacg taatagaaat cggatcagga 120
<210> 560
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 560
gtagactctg tattgattgt tcttgaacga catcaaccat tgatttcaaa gaaggactac 60
aaaaagtatc gatcttttgt ttataagtgg gtaaaccgtg aatatcgtgt tcttttcact 120
<210> 561
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 561
tccttcccaa aacatataaa ctataagata tatggtaata ttccttataa catcagtacg 60
gatattgtca aaagaattac ctttgaaagt caggctaaat atagctatct tatcgttgag 120
<210> 562
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 562
aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg 60
aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg 120
<210> 563
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 563
cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta 60
agcacacaaa ttattaaaaa agtggttttt gaaagccgtg cgtctgacat ctatctgatt 120
<210> 564
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 564
gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg 60
cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa 120
<210> 565
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 565
ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa aatatttggt 60
aatatacctt ataacataag tacggatata atacgcaaaa ttgtttttga tagtatagct 120
<210> 566
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 566
atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat 60
aaaataatga caaatataag attaaatgaa catgataata tctttgaaat cggctcagga 120
<210> 567
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 567
gatgagattt atttaatcgt ggaatacgag tttgctaaaa gattattaaa tacaaaacgc 60
tcattggcat tatttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga 120
<210> 568
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 568
gtggctctgc tggcgcaggc gggggctgag gtatggaaag ataaccgcag tgaaggggcg 60
tcttactatt ttctcgaccc tgacggccat aagctggagc tgcatgtggg gaatctggcg 120
<210> 569
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 569
tcctgtgggg cgctgtggct gtgcttgtcg ctggatgagc agcggcgtaa aacgccccct 60
caggaaagcg actataccca ctacgccttc agcgtggcgg aagaagagtt tgccggggtg 120
<210> 570
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 570
atgctgcagg gattgaatca tctgacgctg gcggtcagcg atctggcgtc aagcctggca 60
ttttatcagc agttacctgg aatgcgcctg cacgccagct gggatagcgg agcctatctc 120
<210> 571
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 571
tttaagcgat atgatgaaaa gtttgccaaa ttcacgccag tgtgtgaagg taatgagctt 60
gccaagtgcg aacatcagtc cttgatcaat gcttatgaca atgccttgct tgccaccgat 120
<210> 572
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 572
gatttcatcg ctcaaagtat ccagtggctg cagacgcaca gcaatgccta tgatgtctca 60
atgctgtatg tcagcgatca tggcgaaagt ctgggtgaga acggtgtcta tctacatggt 120
<210> 573
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 573
caaacctatc ccatcgcgga caatctcggc tttgtgctga cgatcgctgt cgtgctcttt 60
ggcgcgatgc tactgatcac cacgctgtta tcatcgtatc gctatgtgct aaagcctgtg 120
<210> 574
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 574
ataggcatcg ttccaaagaa tgtatctaaa aaagattata aagcaatcgc taaagaacta 60
agtatttctg aagactatat caaacaacaa atggatcaaa attgggtaca agatgatacc 120
<210> 575
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 575
ctccaacatg aagatggcta tcgtgtcaca atcgttgacg ataatagcaa tacaatcgca 60
catacattaa tagagaaaaa gaaaaaagat ggcaaagata ttcaactaac tattgatgct 120
<210> 576
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 576
catctattag gttatgttgg tcccattaac tctgaagaat taaaacaaaa agaatataaa 60
ggctataaag atgatgcagt tattggtaaa aagggactcg aaaaacttta cgataaaaag 120
<210> 577
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 577
tgaattatta gctttagtta gttatcctgc atatgacccc tatgagttta tgttcggcat 60
ttccgacgaa aactacaaaa agatagttaa tgataagaaa gagcccctgt taaataaatt 120
<210> 578
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 578
aatcaaacaa aaggtagggt ttatccatta agagaagcaa cagtacatct tttagggtat 60
gttggagaaa ttaatgctga agaattaaag aataaaaagt ttaaggatta tgatgaacac 120
<210> 579
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 579
aggtataagt gtgaagaatg tagagaatag aggtatgtcg agttataatg caagaaagtt 60
tgccgagata atggatgaat tatatgaaaa tggaaataaa aaatatgaaa tagataggtg 120
<210> 580
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 580
ttgagcaagg tatgcaagat ttgggaatcg gtgaaaatat cccgagtgat tatccctttt 60
ataaagcaca aatctcaaat agtaatttaa aaaatgaaat attattagca gattcaggat 120
<210> 581
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 581
tagaaacatt aaaatcagag cgaggcaaaa taaaagatag aaatggtata gaattagcta 60
aaactggaaa tacatatgaa atcggtattg tccctaacaa aacacccaaa gaaaaatatg 120
<210> 582
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 582
atctggtaca gcattacaac caaaaactgg agaaatttta gctttggtaa gtaccccatc 60
gtacgatgtt tatccattca tgaatggatt aagcaataat gactaccgta aattaactaa 120
<210> 583
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 583
ggatctgcga tggtcttgtc tatggcttca ctagtaggtt ttttacccta tgcggtcttt 60
ggaccagcca ttggtgtatt agtggatcgt catgatagga agaagataat gattggtgct 120
<210> 584
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 584
atggaaaaat acaacaattg gaaacttaag ttttatacaa tatgggcagg gcaggcagta 60
tcattaatca ctagtgccat cctgcaaatg gcgattattt tttaccttac agaaaaaaca 120
<210> 585
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 585
aaaccaaatt tcataagaga aatgaaagaa ggaatggctg tactacggca aaataaagga 60
ttatttgctt tattactcgt tggaacatta tatatgtttg tttatatgcc cataaatgca 120
<210> 586
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 586
gtactggcaa aaccacatta ctaaatatga ttgctcaaaa aacaaaacca gaatctggaa 60
cagttgaaac ggttggcgaa attcaatatt ttgaacagct taacatggat gtggaaaatg 120
<210> 587
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 587
cttgcttatg aagacatgcg tgacgtttca ttattgcaat atttaatgga tgaaacggat 60
tcatcagaat cattcagtag agctatttta aataacttgg gtttaaatga agcacttgat 120
<210> 588
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 588
cgttcttgta atgttttgag tggtggggaa agaacgaaat tatcgttagc agtattattt 60
tcaacgaaag cgaatatgtt aattttggat gaaccaacta attttttaga tattaaaaca 120
<210> 589
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 589
cggatagttc ctgccgagtt tacaacctat ttccggagtg tcattgtctt cggtaaagcc 60
cgtatcctga ccgatgaggt cgagaagcgt gccgctctgc ttcggctggc agagaagtat 120
<210> 590
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 590
tcgtccggcg agtcgggtat gcaagacgag atagacaagg gattcgacca tctggtaatg 60
gtggagataa ccgtcgagca catgacaggc aaggaggcta tacagctggt gcgcagaaag 120
<210> 591
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 591
gtccccgtga gctatgtgta tgccgacggg aagatttatt ttcacggtgc cgtgcaaggg 60
cataagatgg atgccatcag gcagcatccc gaagtctcgt tttgtgtggt ggagcaagac 120
<210> 592
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 592
atgtttagag aaatgcgacg taagcggcaa ttattgccaa cagaagaaag cgttgccatc 60
cttgaaagga tgacgaacgg aacattggct cttcatgggg acgatggtta cccgtatgcc 120
<210> 593
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 593
gttcccatca gttatgtata tgctgatggc aaaatatatt tccatagtgc catgaaaggt 60
cataaagtgg atgccatttt gcagaatgac aaggtatcat tctgcgtggt agaacaggat 120
<210> 594
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 594
gacatcagac cgtctgagtt taccacttac tttcgaagtg tgatagtctt tggcaaagcc 60
cacatattga cggatgaact cgaaaaacgt gttgctttgg gtttattggc agacaagtat 120
<210> 595
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 595
gaagccatga agctttccgc agtcccagtc tatcaggaac ttgcgcgacg tatcggtctt 60
gatctcatgc aaaaagaagt aaaacgtatt ggtttcggta atgctgaaat tggacagcag 120
<210> 596
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 596
tttaaaatgt tgaatgccct gatcggattg gagaaccaga aaacggatat taatgaaata 60
tttaaatgga agggcgagaa aaggtcattt accgcttggg aaaaagacat gacactagga 120
<210> 597
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 597
aatatgcttc ttttagaaga gagtaatggc tacaaaattt ttggaaagac tggttgggca 60
atggatataa aaccacaagt gggctggttg accggctggg ttgagcagcc agatggaaaa 120
<210> 598
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 598
atcaagagct tgcaagacgg actggcctag agctaatgca gaaagaagta aagcgggtta 60
attttggaaa tacaaatatt ggaacacagg tcgataattt ttggttagtt ggccccctta 120
<210> 599
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 599
ttctagtttc tctcagtgca tgttcatcta ttaaaactaa atctgaagat aattttcata 60
tttcttctca gcaacatgaa aaagctatta aaagctattt tgatgaagct caaacacagg 120
<210> 600
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 600
aattacacca gtacaagaag ttaattttgc cgatgacctt gcacataacc gattaccttt 60
taaattagaa actcaagaag aagttaaaaa aatgcttcta attaaagaag taaatggtag 120
<210> 601
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 601
atgcgtgtat tagccttatc ggctgtgttt ttggtggcat cgattatcgg aatgcctgcg 60
gtagcaaagg aatggcaaga aaacaaaagt tggaatgctc actttactga acataaatca 120
<210> 602
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 602
ggcgactata ttattcgggc taaaacggga tactcgacta gaatcgaacc taagattggc 60
tggtgggtcg gttgggttga acttgatgat aatgtgtggt tttttgcgat gaatatggat 120
<210> 603
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 603
cagggcgtag ttgcgctctg gaatgagaat aagcagcaag gatttaccaa taatcttaaa 60
cgggcgaacc aagcattttt acccgcatct acctttaaaa ttcccaatag cttgatcgcc 120
<210> 604
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 604
caagtcgata atttttggct ggtgggtcct ttaaaaatta ctcctcagca agaggcacag 60
tttgcttaca agctagctaa taaaacgctt ccatttagcc aaaaagtcca agatgaagtg 120
<210> 605
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 605
accttcaaaa tgcttaatgc tttgatcggc cttgagcacc ataaggcaac caccacagaa 60
gtatttaagt gggatggtaa aaaaaggcta ttcccagaat gggaaaagaa catgacccta 120
<210> 606
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 606
atgaacatta aagcactctt acttataaca agcgctattt ttatttcagc ctgttcacct 60
tatatagtga ctgctaatcc aaatcacagc gcttcaaaat ctgatgaaaa agcagagaaa 120
<210> 607
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 607
atggatatta ttgataaagt ttttcagcaa gaggatttct catgccagga tttgagtgac 60
agccgttttc gccgctgccg cttttatcag tgtgacttca gccactgtca gctgcaggat 120
<210> 608
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 608
ggtgccaact gctttggcat agagttcagg gagtgcgatc tcaagggcgc caacttttcc 60
cgggcccgct tctacaatca agtcagccat aagatgtact tctgctcggc ttatatctca 120
<210> 609
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 609
cgcggcacct tctcccgcga ctgttggcaa caggtcaatc tgcggggctg tgacctgacc 60
tttgccgatc tggatgggct cgaccccaga cgggtcaacc tcgaaggagt caagatctgt 120
<210> 610
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 610
gcgctgggaa tcgaaattcg ccactgccgc gcacaaggtt cagattttcg cggcgcaagc 60
tttatgaata tgattaccac acgcacctgg ttttgtagcg cctatatcac caataccaac 120
<210> 611
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 611
attggctgtc agttctatga tcgagagagc cagaaagggt gtaattttag tcgcgctatc 60
ctgaaagatg ccattttcaa aagttgtgat ctctccatgg cggatttcag gaatgtgagc 120
<210> 612
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 612
ttttcgtcgt tcgactggcg ggccgcaaac tttacgcact gtgatttgac caattcagaa 60
ctgggcgatc tcgatgtccg gggtgttgat ttgcaaggcg tcaaactgga cagctaccag 120
<210> 613
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 613
ttttcgactt tcgactggcg agcagcaaac ttcacacatt gcgatctgac caattcggag 60
ttgggtgact tagatattcg gcgcgttgat ttacaaggcg ttaagttgga caactaccag 120
<210> 614
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 614
cgaggtgttt tttccgaaga tgtctggggg caatttagcc tacagggtgc caatttatgc 60
cacgccgaac tcgacggttt agatccccgc aaagtcgata catcaggtat caaaattgca 120
<210> 615
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 615
atggaaacct acaatcatac atatcggcac cacaactttt cacataaaga cttaagtggt 60
ctcaccttca ccgcttgcac attcattcgc agcgactttc gacgtgctaa cttgcgtgat 120
<210> 616
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 616
aatgccaatt gctacggtat agagttccgt gcgtgtgatt taaaaggtgc caacttttcc 60
cgaacaaact ttgcccatca agtgagtaat cgtatgtact tttgctcagc atttatttct 120
<210> 617
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 617
caagagtaca agggattcgg catcgttaaa aaacaaggct tagtagttct tgacaatcac 60
aaggcatatc tcatcgacac tccagcttcc gcaggagata ctgaaaagct agtaaactgg 120
<210> 618
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 618
aacgaagttg tctggatacc taataaaaaa atcctattcg ggggctgttt tataaaaccg 60
aatggccttg gcaatctaag tgacgcaaat ttggaagctt ggccaggctc cgcaaaaaaa 120
<210> 619
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 619
aatgaattgc taaataaaaa tggcaaaact caagccaagc actcttttga taaagagagc 60
ttttggttgg tcaaaaataa aattgaaatt ttttatccag gcccaggaca cactcaagat 120
<210> 620
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 620
atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg 60
cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac 120
<210> 621
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 621
ggcgctgact acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg 60
gaaaccctcg cgaaatttcg cagtcgcgac gccagagaca cgcgaccggc ggattccacg 120
<210> 622
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 622
cgacggagcg gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg 60
agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg 120
<210> 623
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 623
atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat 60
ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag 120
<210> 624
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 624
tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag 60
gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg 120
<210> 625
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 625
cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca 60
gtccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca 120
<210> 626
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 626
tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca 60
ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc 120
<210> 627
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 627
caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg 60
gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg 120
<210> 628
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 628
gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg ggttctctat 60
atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt 120
<210> 629
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 629
atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg 60
ttaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac 120
<210> 630
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 630
attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa 60
aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt 120
<210> 631
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 631
gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg 60
ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc 120
<210> 632
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 632
gtgcttggat gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc 60
gttctaaatg aggtcaatac cctgcccggt tttacatcgt acagccgcta cccacgtatg 120
<210> 633
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 633
ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta 60
accaaagtaa acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat 120
<210> 634
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 634
ggaaaaatct taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg 60
aacgaggatg atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc 120
<210> 635
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 635
atcggttgcg gtattttggg caacgactct ttgactgtcg gtgcttgtga cgccatttca 60
ttagtagacg gctttttcga ttttgaagaa aagtaccagc tgatcagcgc caaaatcacc 120
<210> 636
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 636
gcagccattg gcgtacaaag tgctcctacg attctcttga caaatcaagc caaccagcaa 60
gaacaaatcg aagcttttat ccagacccat ggcttcccag ttttctttaa gcctaatgaa 120
<210> 637
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 637
tggttgttgg atacgaaaca taaacagaaa atccagccgc tattcgaagg aaacggcttt 60
tggctaagtg aagagcagca aacgttggta cctgatgttt tatttcccat tatgcatggc 120
<210> 638
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 638
tttgcttacg gttctactgt gttgatccaa aaggcgatag cgggtattga aattggctgc 60
ggcatcttag gaaatgagca attgacgatt ggtgcttgtg atgcgatttc tcttgtcgac 120
<210> 639
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 639
atcgcgccaa caatggattg gtatttgtat caaggaaacc tcgcgaatgt tcgcaatgat 60
acttggctag aagatcacaa aaactgtcac cagctgactt tttctagcca aggatttata 120
<210> 640
<211> 120
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 640
atgaaaaaaa ttgccgtttt atttggaggg aattctccag aatactcagt gtcactagcc 60
tcagcagcaa gtgtgatcca agctattgac ccgctgaaat atgaagtaat gaccattggc 120
Claims (7)
1. A set of capture probes for detecting a pathogenic microorganism, wherein the set of capture probes specifically captures the following pathogenic microorganism genomic coding region sequences: dengue virus 1, dengue virus 2, dengue virus 3, dengue virus 4, human bocavirus 1, human bocavirus 2, human bocavirus 3, human bocavirus 4, influenza a virus H10N1, influenza a virus H1N1, influenza a virus H2N2, influenza a virus H3N2, influenza a virus H5N1, influenza a virus H7N2, influenza a virus H7N7, influenza a virus H9N2, influenza B virus, human adenovirus, human coronavirus 229E, human coronavirus HKU1, human coronavirus NL63, human coronavirus OC43, human respiratory virus 1, human respiratory virus 3, human mumps virus 2, human mumps virus 4, human respiratory syncytial virus a, human respiratory syncytial virus B, rhinovirus a, rhinovirus B, rhinovirus C, enterovirus, human metapneumovirus, human herpesvirus 1, human herpesvirus 2, human herpesvirus 3, human herpesvirus 5, human herpesvirus 6A, human herpesvirus 6B, human herpesvirus 7, human herpesvirus 4, human herpesvirus 8, human paraenterovirus 1, human paraenterovirus 2, human paraenterovirus 3, japanese encephalitis virus, porcine herpesvirus, human parvovirus B19, influenza C, influenza virus, influenza D, enterococcus faecium, enterococcus faecalis, acinetobacter baumannii, serratia marcescens, proteus vulgaris, proteus mirabilis, klebsiella pneumoniae, klebsiella aerogenes, enterobacter cloacae, escherichia coli, haemophilus influenzae, moraxella catarrhalis, pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella oxytoca, streptococcus pyogenes, legionella pneumophila, mycoplasma pneumoniae, chlamydia pneumoniae, listeria monocytogenes, neisseria meningitidis, bordetella pertussis, mycobacterium abscessus, mycobacterium tuberculosis, mycobacterium intracellulare, mycobacterium kansasii, mycobacterium avium, mycobacterium fish, haemophilus parahaemophilus, vibrio parahaemolyticus, vibrio vulnificus, rickettsia typhosa, stenotrophomonas maltophilia, chlamydia psittaci, streptococcus dysgalactiae, mycoplasma hominis, mycoplasma urealyticum, rickettsia praecox, eastern body of notopterygium, clostridium perfringens, streptococcus agalactiae, morganella morganii, corynebacterium diphtheriae, salmonella enterica, candida albicans, candida glabrata, C. Aspergillus fumigatus, aspergillus flavus, aspergillus niger, aspergillus nidulans, aspergillus terreus, yersinia pneumospori, penicillium marneffei, trichomonas faecalis, guangzhou circular nematode, ka Yan Huan spore cocci, trichomonas vaginalis, toxoplasma gondii, taenia catenulatus, baboon Balanx amoeba, hepatitis GB virus type C, human polyomavirus type 2, human polyomavirus type 1, hantavirus, rubella virus, novel bunyavirus, clostridium tetani, cytospora capsulata, chlamydia trachomatis, 2019 novel coronavirus, human immunodeficiency virus type 1, human immunodeficiency virus type 2, rabies virus, human poliovirus type 1, zingiocarb virus, human hepatitis A virus, hepatitis B virus, measles virus, neisseria gonorrhoeae, meisseria, yersinia pestis, bacillus anthracis, mycobacterium leptospirae, schistosoma japonicum, schistosoma mansoni, plasmodium malariae, plasmodium falciparum, amoeba histolytica, leishmania donovani, mycobacterium fortuitum, echinococcus multinomus, human polyomavirus type 3, human polyomavirus type 4, astrovirus, severe acute respiratory distress syndrome-related virus, middle eastern respiratory syndrome coronavirus, hepatitis C virus, leptospira interrogans, vibrio cholerae, human rotavirus type a, human rotavirus type B, human rotavirus type C, brucella canis, nocardia suppurativa, nocardia astrotricha, gandrum glossier, nocardia melitensis and nocardia guinea pig ear inflammation;
The capture probe set comprises probes having sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 517.
2. The set of capture probes for detecting a pathogenic microorganism of claim 1, further comprising probes that specifically capture a drug resistant genomic sequence of: aac6-Ii, aac6, blaCTX-M-151, blaDHA-4, blaIMP-64, blaIMP-37, blaIMP-7, blaKPC-40, blaNDM-9, blaSHV-16, blaTEM-237, blaVIM-32, blaVIM-7, DIM, ermA, ermB, ermC, fosA3, mcr-1, mecA, mecB, mecC, mefA, msrA, nimA, nimB, OXA-23, OXA-24, OXA-48, OXA-51, qnrA9, qnrB12, qnrB85, qnrS11, SIM, sul1, sul2, tetA, vanA, vanB, vanC2 and vanC.
3. The set of capture probes for detecting pathogenic microorganisms according to claim 2, wherein the probes for specifically capturing the sequence of the drug-resistant genome comprise probes having the sequences shown in SEQ ID No. 518 to SEQ ID No. 640.
4. The set of capture probes for detecting a pathogenic microorganism of claim 1, wherein the capture probes are each modified with biotin.
5. A kit for detecting a pathogenic microorganism, comprising the capture probe set of any one of claims 1-4.
6. The kit for detecting a pathogenic microorganism of claim 5, further comprising a positive quality control, a negative quality control, and a library construction reagent.
7. A capture detection method for detecting pathogenic microorganisms for non-diagnostic purposes, characterized in that it comprises the steps of:
library preparation prior to capture: taking a sample to be tested, extracting nucleic acid in the sample, and constructing a library;
liquid phase hybridization capture: taking the library obtained by the construction, adding the capture probe set according to any one of claims 1-4, and performing hybridization capture;
PCR amplification and purification: performing PCR amplification and purification on the hybridization capture product to obtain a capture library;
high throughput sequencing: the capture library was subjected to high throughput sequencing and data analysis.
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