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CN112812966A - Cell culture device - Google Patents

Cell culture device Download PDF

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Publication number
CN112812966A
CN112812966A CN202110142389.3A CN202110142389A CN112812966A CN 112812966 A CN112812966 A CN 112812966A CN 202110142389 A CN202110142389 A CN 202110142389A CN 112812966 A CN112812966 A CN 112812966A
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CN
China
Prior art keywords
opening
cell culture
side plate
plate
space
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Granted
Application number
CN202110142389.3A
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Chinese (zh)
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CN112812966B (en
Inventor
许峰
傅攀峰
何文明
许素玲
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Affiliated Hospital of Medical School of Ningbo University
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Affiliated Hospital of Medical School of Ningbo University
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Priority to CN202110142389.3A priority Critical patent/CN112812966B/en
Publication of CN112812966A publication Critical patent/CN112812966A/en
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Publication of CN112812966B publication Critical patent/CN112812966B/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25—Greenhouse technology, e.g. cooling systems therefor

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a cell culture device, which comprises a culture box, a cell culture space and a cover plate, wherein the cover plate can open the cell culture space on one side; at least one first opening which is arranged on one side of the culture box and communicated with the cell culture space; the adjusting component is movably arranged at the first opening so as to adjust the size of the first opening; a sealing assembly provided to the culture cassette to seal the cell culture space; the cover plate comprises a top plate and a second side plate arranged on the top plate in a surrounding mode, and the first opening is formed in the second side plate. The invention solves the problems that the cell culture plate has single use and can not effectively obstruct external microorganisms, and different demands of cell culture on external environment air in experiments can be met by additionally arranging the adjusting component and the sealing component.

Description

Cell culture device
Technical Field
The invention relates to the technical field of cell culture plates, in particular to a cell culture device.
Background
In the prior art, common cell culture plates or multi-hole plates are usually used for placing cell culture solution for cell culture, and although the cell culture plates on the market have simple structures, the cell culture plates have strong specificity, which means that the requirements of single experiments can only be met, for example, the cell culture plates on the market cannot adjust gas exchange with the external environment; in addition, current cell culture board has the upper cover and establishes with the box body structure, common cooperation mode between the two for the lid, causes the two cooperation from this to have the clearance for some external microorganisms cause the pollution to the cell culture liquid of placing in the box body in getting into the cell culture board through the fit clearance of the two, and then cause the interference to the cell of cultivateing, finally reduces the accuracy of the experimental data that the collection obtained.
Therefore, a sealing property is good, and microorganisms in the external environment can be effectively prevented from entering the sealing structure; and can regulate the air exchange with the external environment, namely, the cell culture device with increased versatility.
Disclosure of Invention
The invention solves the problems that the cell culture plate has single use and can not effectively obstruct external microorganisms, and different demands of different cells for external environment air in the experiment can be met by additionally arranging the adjusting component and the sealing component.
In order to solve the above problems, the present invention provides a cell culture apparatus, comprising a culture box, a cell culture space provided therein, and a cover plate having one side for opening the cell culture space; at least one first opening which is arranged on one side of the culture box and communicated with the cell culture space; the adjusting component is movably arranged at the first opening so as to adjust the size of the first opening; a sealing assembly provided to the culture cassette to seal the cell culture space; the cover plate comprises a top plate and a second side plate arranged on the top plate in a surrounding mode, and the first opening is formed in the second side plate.
In this embodiment, each of the first openings communicates the cell culture space with the external environment, and the adjustment assembly is movably matched with the corresponding first opening to control the adjustment assembly to change the size of the first opening, so as to finally realize the regulation and control of the air flux between the cell culture space and the external environment.
Furthermore, the culture box also comprises a box body, one side of the box body is provided with a second opening, the cover plate is covered on the second opening, and the first side plate of the box body is positioned between the cell culture space and the second side plate of the cover plate; wherein, at least one first opening is arranged on the second side plate.
In this embodiment, each of the first openings is disposed on the second side plate, so that when the air in the external environment and the cell culture space are communicated with each other, dust, impurities, microorganisms, and the like in the external environment are effectively prevented from directly entering the cell culture solution placed in the cell culture space through the first opening, and further, interference is caused on finally acquired experimental data. In this embodiment, the first opening is disposed at one side of the second opening, so that dust, impurities, microorganisms and the like are prevented from directly dropping into the cell culture space during the process of standing the cell culture device.
Further, a first mounting space is arranged between the first side plate and the second side plate; further comprising: the filter screen is arranged in the first installation space and covers each first opening; each adjusting assembly is clamped between the filter screen and the first side plate; wherein each of the first openings communicates with the cell culture space through the first installation space.
In this embodiment, the filter screen covers each first opening, and can effectively filter partial microorganisms and dust in the external environment to enter the cell culture space, and the filter screen is arranged in the first installation space and located between the second side plate and the adjusting component, so as to prevent the adjusting component from controlling the process of the first opening and the filtering action of the filter screen to form interference.
Furthermore, the second side plate is also provided with at least one third opening communicated with the first mounting space; the adjustment assembly further comprises: the adjusting plate is clamped between the filter screen and the first side plate and is arranged opposite to the first opening; the poking block is arranged on one side, exposed to the third opening, of the adjusting plate; wherein the third opening is exposed to air; the dial block is exposed to the third opening; and moving the toggle block to enable the adjusting plate to move in the first mounting space, so that the size of the first opening is changed.
In this embodiment, the poking block can be movably arranged in the third opening, and the poking block is controlled to move in the third opening, so that the adjusting plate connected with the poking block moves in the same direction, and then the size of each first opening is regulated and controlled, so that the requirements for different air in the external environment in an experiment are met. In particular, the adjustment of each of the first openings by the adjustment assembly may be synchronized, or may be adjusted independently of each other.
Further, a first limiting slide rail is arranged on one side, close to the adjusting plate, of the first side plate; the adjusting plate is provided with a first sliding block which is connected with the first limiting sliding rail in a matched manner; wherein, the first slider can slide on the first spacing slide rail.
In this embodiment, the first slider is located the regulating plate is close to one side of first curb plate, first slider through with first spacing slide rail cooperation is connected, and then makes the regulating plate can follow the orbit motion of first spacing slide rail, makes the regulation first open-ended process more reliable and more stable.
Furthermore, the adjusting assembly further comprises a limiting assembly, the limiting assembly is connected with the poking block in a matched mode, and the limiting assembly is arranged on one side, far away from the first side plate, of the second side plate; the spacing subassembly includes: the first limiting parts are arranged on two sides of the third opening; the second limiting piece is connected with the first limiting piece in a matched mode; the second limiting piece limits the position of the toggle block in the third opening; the third opening may extend in a vertical or horizontal direction; the plurality of first limiting parts are arranged on two corresponding sides of the third opening, and the plurality of first limiting parts are arranged in a corresponding direction.
In this embodiment, the second locating part is successively connected with the plurality of first locating parts in a matching manner, so that the toggle block exposed to the third opening can be connected with the second locating part in a matching manner, and according to different experimental requirements, the second locating part can be correspondingly connected with one of the plurality of first locating parts, so that the adjusting plate is prevented from being unable to stabilize the position in the first installation space, and sliding and other conditions occur, thereby affecting the opening degree of the first opening and being not beneficial to later-stage acquisition of experimental data.
Further, the first limiting part is a combined groove; the combination tank includes: the second limiting slide rail is arranged along the same direction of the extension of the third opening; the plurality of third limiting slide rails are communicated with the second limiting slide rails; the arrangement direction of the third limiting slide rails is the same as the extension direction of the second limiting slide rails.
In this embodiment, the plurality of third slide rails are communicated with the second limiting slide rail, the second limiting member can slide on the second limiting slide rail to any one of the third slide rails, and the first limiting member is connected to the dial block in a matching manner. In addition, the second limiting piece is detachably arranged on the combination groove.
Further, the sealing assembly is arranged on the bottom plate of the box body; when the cover plate is covered on the box body, the sealing assembly enables the first side plate to be in sealing fit with the corresponding second side plate.
In this embodiment, the sealing assembly is disposed at a joint between the box body and the cover plate, i.e., seals a fit gap between the first installation space and the bottom plate, and effectively limits air in an external environment from entering the cell culture space from the joint.
Further, the sealing assembly is made of rubber and is provided with a notch, and the second side plate is inserted into the notch.
In this embodiment, the sealing effect of the sealing assembly is further optimized.
Furthermore, the culture box is also provided with at least one layer of heat-insulating layer; the cell culture space is wrapped by the heat preservation layer, and the heat preservation layer is provided with a heat insulation space; wherein the insulating space and the cell culture space are isolated from each other.
In this embodiment, the heat preservation has effectively realized getting in a plurality of placing environment and has put during the cell culture device, place in a transition environment of cell culture liquid in the cell culture space, when avoiding removing in a plurality of temperature environment, because the cell of cultivateing in the cell culture liquid can't adapt to the temperature variation fast and cause the influence to the culture result, is unfavorable for the accurate collection of final experimental data.
After the technical scheme of the invention is adopted, the following technical effects can be achieved:
(1) each first opening is communicated with the cell culture space and the external environment, and the adjusting assembly is movably matched with the corresponding first opening to control the adjusting assembly so as to change the size of the first opening, and finally the air exchange quantity between the cell culture space and the external environment is regulated and controlled;
(2) the filter screen covers each first opening, partial microorganisms, dust and the like in the external environment can be effectively filtered to enter the cell culture space, and meanwhile, the filter screen is arranged in the first installation space and is positioned between the second side plate and the adjusting component, so that interference between the process of controlling the first openings by the adjusting component and the filtering action of the filter screen is avoided;
(3) the heat preservation has effectively realized getting in a plurality of placing environment when putting the cell culture device, has given place in a transition environment of cell culture liquid in the cell culture space, when avoiding removing in a plurality of temperature environment, because cell culture liquid temperature takes place rapid change and causes the influence to the cell culture result, is unfavorable for the accurate collection of final experimental data.
Drawings
Fig. 1 is a schematic structural diagram of a cell culture apparatus 100 according to a first embodiment of the present invention.
Fig. 2 is an exploded view of fig. 1.
FIG. 3 is a schematic view of the cell culture apparatus 100 shown in FIG. 1 from another perspective.
Fig. 4 is a sectional view taken in the direction of a-a shown in fig. 3.
Fig. 5 is a partially enlarged view of fig. 3.
FIG. 6 is a schematic view of another structure of the cell culture apparatus 100 according to the first embodiment of the present invention.
Fig. 7 is a partial view of a portion at B shown in fig. 6.
Fig. 8 is a partial enlarged view of fig. 6 at a position B.
Fig. 9 is a partial enlarged view of a further portion B shown in fig. 6.
FIG. 10 is a schematic structural diagram of a cell culture apparatus 100 according to a second embodiment of the present invention.
Fig. 11 is a partial enlarged view of fig. 10 at C.
Description of reference numerals:
100-a cell culture device; 10-a culture box; 11-cell culture space; 12-a cover plate; 121-a second side plate; 122-a third opening; 13-a cartridge; 131-a second opening; 132-cell culture well slots; 133-a first side panel; 1331-a first stop slide; 14-a first installation space; 20-a first opening; 30-an adjustment assembly; 31-a toggle block; 32-adjusting plate; 321-a first slider; 33-a stop assembly; 331-a first limiting member; 3311-a second limit slide rail; 3312-third limit slide rail; 332-a second stop; 34-a fourth limiting slide rail; 35-a fourth slider; 36-a shield; 40-sealing the assembly.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
The first embodiment is as follows:
referring to fig. 1-9, fig. 1 is a schematic structural diagram of a cell culture apparatus 100 according to an embodiment of the present invention. The cell culture apparatus 100 includes, for example, a culture cassette 10, at least one first opening 20, at least one regulating member 30, and a sealing member 40. A cell culture space 11 and a cover plate 12 with one side capable of opening the cell culture space 11 are arranged in the culture box 10; each of the first openings 20 is opened at one side of the culture cassette 10 and communicates with the cell culture space 11; each adjusting component 30 is movably matched and connected with each first opening 20 so as to adjust the size of the first opening 20; the sealing member 40 is provided at one side of the culture cassette 10 to seal the cell culture space 11.
Specifically, the culture cassette 10 further includes a cassette body 13, for example, one side of the cassette body 13 is a second opening 131, and the cover plate 12 is placed over the second opening 131 to form the cell culture space 11 having the plurality of first openings 20. Wherein the case body 13 is provided with a plurality of cell culture well grooves 132 isolated from each other, each cell culture well groove 132 is provided in the cell culture space 11, and the second opening 131 faces the opening direction of each cell culture well groove 132.
The box 13 has a first side plate 133, and the cover 12 has a second side plate 121. When the cover plate 12 is in cover fit with the box body 13, the first side plate 133 and the second side plate 121 are oppositely arranged, the first side plate 133 is located between the second side plate 121 and the cell culture space 11, each first opening 20 is opened on the second side plate 121, and preferably, each first opening 20 is circumferentially and uniformly distributed on the second side plate 121. For example, the size of the opening of each first opening 20 is different, and the sizes of the openings are gradually distributed, wherein the arrangement of each first opening 20 on the second side plate 121 may be along the horizontal direction or along the vertical direction. Each regulating component 30 is matched and connected with each first opening 20 with different calibers, so that the communication condition between the external environment and the cell culture space 11 is changed.
The at least one first opening 20 may be separate from or in communication with each other. For example, box body 13 is first cuboid casing, and apron 12 is the second cuboid casing, second cuboid casing lid is located the outside of first cuboid casing, the second cuboid casing is located by closing cap board and circumference the second curb plate 121 of closing cap encloses. The second side plate 121 is formed by splicing four end-to-end side plates, and each first opening 20 is communicated with each other or separately arranged on the side plates.
Of course, the shape of each first opening 20 may be circular or rectangular. Preferably, each of the first openings 20 has a rectangular shape with a rectangular edge. For example, a scale may be marked on a frame of each of the edge rectangles, and when the adjustment assembly 30 is controlled to adjust the opening/closing degree of each of the first openings 20, the scale on the frame of the edge rectangle may be compared with reference according to specific requirements of an experiment, so that the adjustment accuracy can be more accurately and clearly grasped, and the accuracy of acquiring experimental data can be improved.
Further, a first installation space 14 is provided between the first side plate 133 and the corresponding second side plate 121. The cell culture apparatus 100 further includes, for example, a filter screen (not shown). The filter screen is provided in the first installation space 14 and covers each of the first openings 20. Specifically, the filter screen may be disposed on the second side plate 121, and each of the first openings 20 is communicated with the cell culture space 11 through the first installation space 14. For example, the filter screen and the second side plate 121 are detachably connected, that is, the second side plate 121 is provided with an adhesive structure, and a matching member is disposed at a position opposite to the filter screen and is in matching connection with the adhesive structure, so that after long-term use, the filter screen can be detached from the second side plate 121, and the cell culture apparatus 100 can be put into use by replacing with a new filter screen.
Preferably, the filter screen may be a graphene filter screen. When the air of the external environment can penetrate through the graphene filter screen, the body of bacteria or viruses doped in the air is damaged, and then only the gas is allowed to enter the cell culture space 11.
Preferably, the second side plate 121 is further opened with at least one third opening 122 communicating with the first mounting space 14. The adjustment assembly 30 includes, for example, an adjustment plate 32 and a dial block 31. The adjusting plate 32 is clamped between the filter screen and the first side plate 133, and the adjusting plate 32 is arranged opposite to the first opening 20; the dial block 31 is disposed on a side of the adjustment plate 32 exposed to the third opening 122. Wherein the third opening 122 communicates with the cell culture space 11 via the first installation space 14. The dial block 31 is moved along the opening direction of the third opening 122, so as to drive the adjusting plate 32 to move in the first mounting space 14, and further change the size of the opening formed by the matching of the adjusting plate 32 and each first opening 20.
Preferably, a first limiting slide rail 1331 is disposed on one side of the first side plate 133 close to the adjusting plate 32, and a first sliding block 321 is disposed on one side of the adjusting plate 32 close to the first side plate 133. The first sliding block 321 is connected with the first limit sliding rail in a sliding manner. For example, the adjusting plate 32 is a deformable frame-shaped rubber strip, the frame-shaped rubber strip is sleeved outside the first side plate 133, the first limiting slide rail 1331 is a long strip-shaped slide groove, when the long strip-shaped slide groove is arranged in the horizontal direction, the third opening 122 may also be distributed in the same manner as the long strip-shaped slide groove, and the first slider 321 slides in the long strip-shaped slide groove along the length direction thereof, so as to drive the frame-shaped rubber strip to horizontally move along the circumferential direction of the first side plate 133. At this time, the first sliding block 321 is connected to the horizontally arranged long sliding groove in a matching manner, so that the frame-shaped rubber strip does not slide down due to gravity operation, thereby affecting the stability of the rubber strip in the first installation space 14.
In contrast, when the elongated sliding groove and the third opening 122 both extend in the vertical direction, the same situation as the horizontal arrangement is used, and the details are not repeated here.
Preferably, the adjustment assembly 30 further comprises a limit assembly 33, for example. The limiting component 33 is arranged on one side of the second side plate 121 far away from the first side plate 133, and the limiting component 33 is matched and connected with the poking block 31. Specifically, the position limiting assembly 33 includes, for example, a second position limiting member 332 and a plurality of first position limiting members 331. The first limiting members 331 are disposed at two sides of the third opening 122; the second limiting members 332 are connected to one or more first limiting members 331 in a matching manner. The second limiting member 332 limits the position of the dial block 31 in the third opening 122; the third opening 122 may extend in a vertical direction or a horizontal direction, and the corresponding first limiting members 331 may be disposed at two sides of the third opening 122 along a length direction thereof, and the first limiting members are arranged in the direction.
Further, the first limiting members 331 are combined slots. The combination slots include, for example, a second limit slide 3311 and a plurality of third limit slides 3312. The second limit slide rails 3311 are distributed along the same direction in which the third openings 122 extend; the plurality of third limiting slide rails 3312 communicate with the second limiting slide rail 3311.
For example, the third opening 122 extends along the vertical direction, and the second position-limiting slide rail 3311 is disposed on one side of the third opening 122 in the vertical direction and extends in the same direction; the plurality of third limiting slide rails 3312 are disposed at two sides of the third opening 122 along the length direction thereof, and the plurality of third limiting slide rails 3312 are communicated with the second limiting slide rails 3311, and each third limiting slide rail 3312 is disposed in the horizontal direction or at a certain angle with the vertical direction; the second position-limiting members 332 are slider structures and can slide on the second position-limiting slide rails 3311 and then move to the corresponding third position-limiting slide rails 3312. At this time, the shifting block 31 is arranged on the sliding block to prevent the adjusting plate 32 from sliding downwards under the action of gravity. In addition, each third limiting slide rail 3312 may be arranged at equal intervals, so that the operator can adjust the shifting block 31 according to the degree of the adjusting plate 32 corresponding to the third limiting slide rail 3312 at different positions to shield the first opening 20, i.e., the degree of shielding of the adjusting plate 32 to the first opening 20 can be accurately determined by directly observing the matching position of the second limiting member 332 and the corresponding third limiting slide rail 3312 each time.
In contrast, the third opening 122 may also extend along the horizontal direction, and the specific structure thereof is similar to that of the above embodiments, and is not described herein again.
In a specific embodiment, when the experimental subject is applied to the aquatic or plant cell culture solution, since the aquatic or plant cell culture solution needs a carbon dioxide environment, so that the aquatic or plant cells are in the carbon dioxide environment, and the external environment does not need to be communicated with the cell culture space 11, the adjusting plate 32 can completely block each first opening 20 by the movable toggle block 31, so that the cell culture space 11 is in a closed state; in addition, when the subject is a cell culture solution mainly involving aerobic respiration, for example, when culturing animal cells, it is necessary to communicate the external environment with the cell culture space 11, and to adjust the dial block 31 so that the adjustment plate 32 partially blocks or completely opens each of the first openings 20.
Further, a sealing member 40 is provided on the bottom plate of the case 13. When the cover 12 is covered on the box 13, the sealing assembly 40 makes the first side plate 133 and the corresponding second side plate 121 in sealing engagement.
Preferably, the sealing assembly 40 is made of rubber, and is disposed on the bottom plate and extends toward the direction close to the second side plate 121, and is provided with a notch opening toward the cover plate 12, and the notch is inserted and matched with the end portion of the second side plate 121.
Further, the cell culture apparatus 100 may further include a snap structure (not shown). The snap structure is disposed between the second side plate 121 and the sealing assembly 40. Specifically, the fastening structure includes, for example, a first fastening member and a second fastening member, the first fastening member is disposed on one side of the sealing portion 40 close to the second side plate 121, the second fastening member is disposed at a position opposite to the second side plate 121, and when the first fastening member is coupled to the second fastening member, the cover plate 12 can be stably sleeved on the box body 13, so as to prevent the cover plate 12 from moving relative to the box body 13 and even the cover plate 12 is separated from the box body 13 when the cell culture apparatus 100 is in a dynamic environment. For example, the sealing assembly 40 is made of deformable rubber, the first fastener is a triangular protrusion, and the second fastener is a triangular groove. Poking towards the direction away from the first side plate 133 by controlling the sealing assembly 40, and then sleeving the cover plate 12 to the box body 13, loosening the control on the sealing assembly 40, so that the first buckle part is matched with the second buckle part to complete the limit matching connection between the first buckle part and the second buckle part.
Of course, the first fastener and the second fastener may also be structures of other forms, and details are not repeated here.
Preferably, the culture box 10 is further provided with at least one insulating layer, for example. The heat-insulating layer wraps the cell culture space 11. Each heat preservation is equipped with thermal-insulated space in the heat preservation, thermal-insulated space and cell culture space mutual isolation. For example, the heat preservation layer effectively realizes that a transition environment is provided for the cell culture solution placed in the cell culture space 11 when the cell culture device 100 is taken and placed in a plurality of placing environments, and avoids the influence on the culture result caused by the fact that cells in the cell culture solution cannot adapt to temperature change quickly when the cells move in a plurality of temperature environments, which is not beneficial to the collection of final experimental data.
Example two:
referring to fig. 10-11, the present embodiment differs from the first embodiment in that the adjustment assembly 30 of the present embodiment is disposed on a side of the first side plate 133 away from the second side plate 121. The adjustment assembly 30 enables a single adjustment to the at least one first opening 20.
Specifically, the adjusting assembly 30 includes, for example, a fourth limit slide 34 and at least one fourth slider 35. Each fourth slider 35 is slidably disposed on the fourth limiting slide rail 34, so as to shield each first opening 20, thereby adjusting the size of the opening of each first opening 20.
Preferably, the adjustment assembly 30 is also provided with a shutter 36, for example. The shielding piece 36 is disposed on a side of the fourth limiting slide rail 34 away from the fourth slide block 35, and the shielding piece 36 is used for preventing the fourth slide block 35 from separating from the fourth limiting slide rail 34 when sliding thereon. For example, the fourth slider 35 is an arc-shaped slider, and the arc-shaped slider is in line contact with the fourth limit slide rail 34, so that the moving efficiency of the arc-shaped slider on the fourth limit slide rail 34 is improved to a certain extent.
Of course, the fourth slider 35 may also be a frame-shaped slider, the frame-shaped slider is an elastic member, and is circumferentially disposed on the second side plate 121, and the position of the elastic member is adjusted, so that the plurality of first openings 20 can be simultaneously shielded, and the size of the opening between the plurality of first openings 20 and the cell culture space 11 can be adjusted.
Although the present invention is disclosed above, the present invention is not limited thereto. Various changes and modifications may be effected therein by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (10)

1. A cell culture apparatus, comprising:
the culture box is internally provided with a cell culture space and a cover plate with one side capable of opening the cell culture space;
at least one first opening which is arranged on one side of the culture box and communicated with the cell culture space;
the adjusting component is movably arranged at the first opening so as to adjust the size of the first opening;
a sealing assembly provided to the culture cassette to seal the cell culture space;
the cover plate comprises a top plate and a second side plate arranged on the top plate in a surrounding mode, and the first opening is formed in the second side plate.
2. The cell culture apparatus according to claim 1,
the culture box also comprises a box body, one side of the box body is provided with a second opening, the cover plate is covered on the second opening, and the first side plate of the box body is positioned between the cell culture space and the second side plate of the cover plate;
wherein, at least one first opening is arranged on the second side plate.
3. The cell culture apparatus of claim 2, wherein a first mounting space is provided between the first side plate and the second side plate; further comprising:
the filter screen is arranged in the first installation space and covers each first opening;
each adjusting assembly is clamped between the filter screen and the first side plate;
wherein each of the first openings communicates with the cell culture space through the first installation space.
4. The cell culture device of claim 3, wherein the second side plate is further provided with at least one third opening communicated with the first installation space; the adjustment assembly further comprises:
the adjusting plate is clamped between the filter screen and the first side plate and is arranged opposite to the first opening;
the poking block is arranged on one side, exposed to the third opening, of the adjusting plate;
wherein the third opening is exposed to air; the dial block is exposed to the third opening; and moving the toggle block to enable the adjusting plate to move in the first mounting space, so that the size of the first opening is changed.
5. The cell culture device of claim 4, wherein a first limiting slide rail is arranged on one side of the first side plate close to the adjusting plate; the adjusting plate is provided with a first sliding block which is connected with the first limiting sliding rail in a matched manner;
wherein, the first slider can slide on the first spacing slide rail.
6. The cell culture device of claim 4 or 5, wherein the adjustment assembly further comprises a limiting assembly, the limiting assembly is connected with the dial block in a matching manner, and the limiting assembly is arranged on one side of the second side plate away from the first side plate; the spacing subassembly includes:
the first limiting parts are arranged on two sides of the third opening;
the second limiting piece is connected with the first limiting piece in a matched mode;
the second limiting piece limits the position of the toggle block in the third opening; the third opening may extend in a vertical or horizontal direction; the plurality of first limiting parts are arranged on two corresponding sides of the third opening, and the plurality of first limiting parts are arranged in a corresponding direction.
7. The cell culture apparatus according to claim 6, wherein the plurality of first stoppers are combined grooves; the combination tank includes:
the second limiting slide rail is arranged along the same direction of the extension of the third opening;
the plurality of third limiting slide rails are communicated with the second limiting slide rails;
the arrangement direction of the third limiting slide rails is the same as the extension direction of the second limiting slide rails.
8. The cell culture apparatus of claim 2, wherein the sealing assembly is disposed on a bottom plate of the cassette body;
when the cover plate is covered on the box body, the sealing assembly enables the first side plate to be in sealing fit with the corresponding second side plate.
9. The cell culture apparatus according to claim 8,
the sealing assembly is made of rubber and is provided with a notch, and the second side plate is inserted into the notch.
10. The cell culture device of claim 1, wherein the culture box is further provided with at least one layer of heat insulation; the cell culture space is wrapped by the heat preservation layer, and the heat preservation layer is provided with a heat insulation space;
wherein the insulating space and the cell culture space are isolated from each other.
CN202110142389.3A 2021-02-02 2021-02-02 Cell culture device Active CN112812966B (en)

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US5817509A (en) * 1997-03-19 1998-10-06 Becton Dickinson And Company Culture vessel assembly
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WO2003085080A1 (en) * 2002-04-04 2003-10-16 Corebiotech Co., Ltd. Cell culture plate and system using the same
US20090191620A1 (en) * 2008-01-25 2009-07-30 Martin Gregory R Limited access multi-layer cell culture system
CN201762333U (en) * 2010-09-07 2011-03-16 中国航天员科研训练中心 Open type cell culture box
CN207483768U (en) * 2017-09-21 2018-06-12 广州洁特生物过滤股份有限公司 A kind of cell culture case
CN111676135A (en) * 2020-06-23 2020-09-18 西安中美弘康生物科技有限公司 A multipurpose cell culture box

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4657867A (en) * 1984-11-01 1987-04-14 Becton, Dickinson And Company Multiwell tissue culture assembly with features for reduced media evaporation
US5817509A (en) * 1997-03-19 1998-10-06 Becton Dickinson And Company Culture vessel assembly
US5863792A (en) * 1997-03-19 1999-01-26 Becton Dickson And Company Culture vessel assembly
WO2003085080A1 (en) * 2002-04-04 2003-10-16 Corebiotech Co., Ltd. Cell culture plate and system using the same
US20090191620A1 (en) * 2008-01-25 2009-07-30 Martin Gregory R Limited access multi-layer cell culture system
CN101978041A (en) * 2008-01-25 2011-02-16 康宁股份有限公司 Limited access multi-layer cell culture system
CN201762333U (en) * 2010-09-07 2011-03-16 中国航天员科研训练中心 Open type cell culture box
CN207483768U (en) * 2017-09-21 2018-06-12 广州洁特生物过滤股份有限公司 A kind of cell culture case
CN111676135A (en) * 2020-06-23 2020-09-18 西安中美弘康生物科技有限公司 A multipurpose cell culture box

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