CN112760211A - Animal coccidian oocyst separation and culture device - Google Patents
Animal coccidian oocyst separation and culture device Download PDFInfo
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Abstract
本发明公开了一种动物球虫卵囊分离、培养装置，它涉及生物分离技术领域。将网筛放到一号筒上，盖好，将饱和食盐水倒入盛有粪便的器皿内，搅拌摇匀，倒入网筛上，筒体内倒入饱和食盐水，打开磁力搅拌器搅拌，静止后将上清液放到二号筒的筒体中，加入饱和食盐水，打开磁力搅拌器搅拌，静止后将上清液放到三号筒中，加入饱和食盐水，既为分离的球虫卵囊，后倒入离心管中离心，加重铬酸钾，倒入培养装置中，同时利用加氧装置进行加氧气，2‑3天即可得到分离培养的孢子化卵囊，用于试验研究。本发明操作简单，虫卵分离效果明显，能分离收集大量虫卵，利于对抗球虫药物的研发和筛选，同时消毒方便，可重复使用，应用前景广阔。
The invention discloses an animal coccidia oocyst separation and culture device, which relates to the technical field of biological separation. Put the mesh sieve on the No. 1 cylinder, cover it, pour the saturated brine into the container containing the feces, stir and shake well, pour it on the mesh sieve, pour the saturated brine into the cylinder, turn on the magnetic stirrer, stir, and stand still. Then put the supernatant into the cylinder of No. 2 cylinder, add saturated saline, turn on the magnetic stirrer and stir, put the supernatant into the No. 3 cylinder after static, add saturated saline, which is the separated coccidial oocyst. , and then poured into a centrifuge tube for centrifugation, added potassium chromate, poured into a culture device, and at the same time added oxygen with an oxygenation device, and isolated and cultured sporulated oocysts were obtained in 2-3 days for experimental research. The invention is simple to operate, has obvious egg separation effect, can separate and collect a large number of eggs, is beneficial to the research and development and screening of anticoccidial drugs, is convenient for disinfection, can be reused, and has broad application prospects.
Description
Technical Field
The invention relates to the technical field of bioseparation, in particular to a device for separating and culturing animal coccidian oocysts.
Background
Coccidiosis of chicken is caused by Eimeria, the disease is most susceptible to chicks of 15-45 days old, the morbidity is generally as high as 50% -70%, the mortality is 20% -30%, the severity can reach 80%, and most adult chickens carry insects. Eimeria infects intestinal epithelial cells mainly, so that the egg yield is reduced, the growth is slow, and the feed conversion rate is low. Global economic loss of 30 hundred million dollars is caused by chicken coccidiosis every year, direct and indirect economic loss caused by the coccidiosis in Poland is about 5100 ten thousand Euros every year, the cost of anti-coccidiosis drugs paid out every year in China is about 6-8 hundred million yuan RMB, which accounts for nearly one third of the total prevention and treatment cost of the chicken diseases, and the data also rises sharply along with the rapid development of intensive chicken raising industry in China.
The research of chicken coccidiosis in veterinary clinics is mainly the research of coccidian oocyst pathogens, but the research on the separation of oocysts in excrement is less at present, the separation of coccidian oocysts in laboratories is relatively coarse, the next research is usually carried out by filtering once by using a 60-mesh or 80-mesh screen, and only filtering can cause food residues or small-particle excrement in filtrate and oocyst inclusion, wherein certain difficulty and uncertainty are caused to the subsequent research work.
At present, chicken coccidiosis has serious harm to the chicken industry, so the research and development and the screening of anti-coccidiosis drugs are particularly important, and the screening and the development of the drugs are based on the separation of purified and cultured oocysts, so the design of an animal coccidiosis oocyst separation and culture device is particularly necessary.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the animal coccidian oocyst separation and culture device which is simple in structure, reasonable in design, simple and convenient to operate, obvious in egg separation effect, capable of separating and collecting a large number of eggs, beneficial to research, development and screening of anticoccidial drugs, strong in practicability and easy to popularize and use.
In order to achieve the purpose, the invention is realized by the following technical scheme: an animal coccidian oocyst separation and culture device comprises a magnetic stirrer, a first number cylinder, a second number cylinder, a third number cylinder, a centrifugal machine, an insect egg culture device and an automatic oxygenation device, wherein the magnetic stirrer comprises an object stage, a time controller, a magnetic stirrer, a first base and magnetic beads, the object stage is arranged on the first base, and the time controller and the magnetic stirrer are installed on an operation panel of the first base; the first horn comprises a first horn cover, a first horn body and a first switch, the first horn cover is arranged at the top end of the first horn body, the first switch is arranged at the bottom of the first horn body, and a 60-mesh or 80-mesh screen can be clamped between the first horn cover and the first horn body; the second horn comprises a second barrel body and a second switch, and the bottom of the second barrel body is provided with the second switch; the third cylinder comprises a third cylinder body and a third switch, and the bottom of the third cylinder body is provided with the third switch; the centrifuge comprises a second base, a socket, a device switch, a rotor clamping seat, a control panel, a top cover, a rotor and a main body, wherein the main body is arranged on the second base; the worm egg culture device comprises a fourth cylinder body, a fourth cylinder cover and a rotary cover, wherein the fourth cylinder cover is arranged at the top end of the fourth cylinder body, and the rotary cover is arranged on the fourth cylinder cover in a rotatable manner; the automatic oxygen adding device comprises a micro gas reservoir, a pipeline and a gas quantity switch, wherein the micro gas reservoir is connected with the pipeline, and the gas quantity switch is arranged on the pipeline.
Preferably, the first barrel cover in the first barrel is a hollow cover, the diameter of the first barrel cover is 10cm, the first barrel cover is connected to the top wall of the first barrel in a clamped mode, the first barrel is a glass barrel, the height of the wall of the first barrel is 20cm, the diameter of the first barrel is 10cm, scales which are 1cm in unit and 20cm in total are arranged on the barrel wall of the first barrel, an opening is formed in the barrel wall which is 10cm away from the bottom of the first barrel, the opening is connected with the barrel wall, the width of the opening is 2cm, and the first switch is arranged at the position, 1cm away from the barrel wall, of the opening.
Preferably, the second cylinder in the second cylinder is a glass cylinder, the wall height is 20cm, the diameter is 10cm, the cylinder wall of the second cylinder is provided with scales which are 20cm in total and take 1cm as a unit, the cylinder wall which is 10cm away from the bottom of the second cylinder is provided with an opening, the opening is connected with the cylinder wall and has a width of 2cm, and the second switch is arranged at a position of the opening which is 1cm away from the cylinder wall.
Preferably, the third cylinder in the third cylinder is a glass cylinder, the wall height is 20cm, the diameter is 10cm, the cylinder wall of the third cylinder is provided with scales of 20cm in total by taking 1cm as a unit, the cylinder wall 10cm away from the bottom of the third cylinder is provided with an opening, the opening is connected with the cylinder wall and has a width of 2cm, and the third switch is arranged at a position 1cm away from the cylinder wall.
Preferably, the main body of the centrifuge is a stainless steel cuboid, and a control panel is provided with a time controller, a rotating speed controller, a start and pause key and a cover controller switch; the rotor is a conical rotor with a large upper part and a small lower part, 12 centrifugal grooves are arranged in the rotor, and clamping grooves which are in sawtooth-shaped fit with the rotor clamping seats are formed in the bottom end of the rotor.
Preferably, the fourth cylinder in the worm egg culture device is a glass cylinder, the wall height is 5cm, the diameter is 10cm, the cylinder wall of the fourth cylinder is provided with scales with 1cm as a unit, and the fourth cylinder cover is a stainless steel cover.
Preferably, the miniature gas reservoir in the automatic oxygen adding device adopts a stainless steel cylinder gas reservoir with the diameter of 10cm and the height of 20cm, the pipeline adopts a plastic pipeline, and an adjustable gas quantity switch is arranged on the pipeline at a position 12cm away from the gas reservoir.
The invention has the beneficial effects that: this device easy operation, the worm ovum separation effect is obvious, can separate at every turn and collect a large amount of worm ova for experimental study does benefit to the research and development and the screening of anti coccidian medicine, and the device disinfection is convenient simultaneously, repeatedly usable, and is practical reliable, and application prospect is wide.
Drawings
The invention is described in detail below with reference to the drawings and the detailed description;
FIG. 1 is a schematic structural view of a magnetic stirrer according to the present invention;
FIG. 2 is a schematic view of a horn according to the present invention;
FIG. 3 is a schematic structural diagram of a second horn according to the present invention;
FIG. 4 is a schematic structural diagram of a third horn according to the present invention;
FIG. 5 is a schematic view of the centrifuge of the present invention;
FIG. 6 is a schematic structural diagram of a control panel in the centrifuge of the present invention;
FIG. 7 is a schematic plan view of a rotor in the centrifuge of the present invention;
FIG. 8 is a schematic perspective view of a rotor of the centrifuge of the present invention;
FIG. 9 is a schematic structural view of an egg culture apparatus according to the present invention;
FIG. 10 is a schematic structural view of a fourth cylinder cover in the insect egg culture apparatus according to the present invention;
FIG. 11 is a schematic view of the automatic oxygen supplier according to the present invention;
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Referring to fig. 1 to 11, the following technical solutions are adopted in the present embodiment: an animal coccidian oocyst separation and culture device comprises a magnetic stirrer 1, a first number cylinder 2, a second number cylinder 3, a third number cylinder 4, a centrifuge 5, an egg culture device 6 and an automatic oxygenation device 7, wherein the magnetic stirrer 1 comprises an object stage 101, a time controller 102, a magnetic stirrer 103, a first base 104 and magnetic beads 105, the object stage 101 is arranged on the first base 104, and the time controller 102 and the magnetic stirrer 103 are arranged on an operation panel of the first base 104; the first horn 2 comprises a first horn cover 201, a first barrel 202 and a first switch 203, the first horn cover 201 is arranged at the top end of the first barrel 202, the first switch 203 is arranged at the bottom of the first barrel 202, and a 60-mesh or 80-mesh screen can be clamped between the first horn cover 201 and the first barrel 202; the second horn 3 comprises a second cylinder 301 and a second switch 302, and the second switch 302 is installed at the bottom of the second cylinder 301; the third horn 4 comprises a third barrel 401 and a third switch 402, and the bottom of the third barrel 401 is provided with the third switch 402; the centrifuge 5 comprises a second base 501, a socket 502, a device switch 503, a rotor clamping seat 504, a control panel 505, a top cover 506, a rotor 507 and a main body 508, wherein the main body 508 is installed on the second base 501, the socket 502 and the device switch 503 are installed on the side edge of the main body 508, the control panel 505 is arranged on the main body 508, a rotor space is reserved in the front half part of the main body 508, the rotor 507 is installed in the main body 508 through the rotor clamping seat 504, and the top end of the main body 508 is provided with the top cover 506; the worm egg culture device 6 comprises a fourth cylinder 601, a fourth cylinder cover 602 and a rotary cover 603, wherein the fourth cylinder cover 602 is arranged at the top end of the fourth cylinder 601, and the rotary cover 603 is arranged on the fourth cylinder cover 602 in a rotatable manner; the automatic oxygen adding device 7 comprises a micro gas reservoir 701, a pipeline 702 and a gas quantity switch 703, wherein the pipeline 702 is connected to the micro gas reservoir 701, and the gas quantity switch 703 is installed on the pipeline 702.
Specifically, the structures of the components are as follows: first cover 201 in a number section of thick bamboo 2 is hollow lid, and the diameter is 10cm, and first cover 201 joint is on the roof of first barrel 202, and first barrel 202 is the glass barrel, and the wall height is 20cm, and the diameter is 10cm, is provided with on the section of thick bamboo wall of first barrel 202 and uses 1cm as the unit, totally 20 cm's scale, is provided with the opening on the section of thick bamboo wall apart from first barrel 202 bottom 10cm, and this opening links to each other with the section of thick bamboo wall, and the width is 2cm, and first switch 203 sets up in opening distance section of thick bamboo wall 1cm department.
The second cylinder 301 in the second cylinder 3 is a glass cylinder, the wall height is 20cm, the diameter is 10cm, the cylinder wall of the second cylinder 301 is provided with scales which are 20cm in total and take 1cm as a unit, the cylinder wall which is 10cm away from the bottom of the second cylinder 301 is provided with an opening, the opening is connected with the cylinder wall, the width is 2cm, and the second switch 302 is arranged at the position of the opening which is 1cm away from the cylinder wall.
The third cylinder 401 in the third cylinder 4 is a glass cylinder, the wall height is 20cm, the diameter is 10cm, the cylinder wall of the third cylinder 401 is provided with scales which are 20cm in total and take 1cm as a unit, the cylinder wall which is 10cm away from the bottom of the third cylinder 401 is provided with an opening, the opening is connected with the cylinder wall and has the width of 2cm, and the third switch 402 is arranged at the position of 1cm away from the cylinder wall.
A main body 508 in the centrifuge 5 is a stainless steel cuboid, and a time controller a, a rotating speed controller b, a start and pause key c and a cover controller switch d are arranged on a control panel 505; the rotor 507 is a conical rotor with a large upper part and a small lower part, 12 centrifugal grooves are arranged in the rotor 507, and the bottom end of the rotor 507 is provided with a clamping groove 509 which is in sawtooth-shaped fit with the rotor clamping seat 504.
The fourth cylinder 601 in the worm egg culture device 6 is a glass cylinder with a wall height of 5cm and a diameter of 10cm, the cylinder wall of the fourth cylinder 601 is provided with scales with 1cm as a unit, and the fourth cylinder cover 602 is a stainless steel cover.
The micro gas reservoir 701 in the automatic oxygen adding device 7 is a stainless steel cylinder gas reservoir with the diameter of 10cm and the height of 20cm, the pipeline 702 is a plastic pipeline, and an adjustable gas amount switch 703 is arranged on the pipeline 702 at a position 12cm away from the gas reservoir.
The work flow of the specific embodiment is as follows:
(1) spreading a mesh screen of 80 meshes on the top end of a first cylinder 202 of a first cylinder 2, covering a first cylinder cover 201 on the top wall of the first cylinder 202, pouring a proper amount of saturated salt solution into a container containing excrement, stirring and shaking uniformly, pouring the excrement uniformly mixed with the saturated salt solution onto a mesh screen, taking off the first cylinder cover 201 and the mesh screen after the screening is finished, and cleaning;
(2) placing the magnetic beads 105 into a first cylinder 2, pouring saturated salt solution into the cylinder to a position of 15cm, then placing the cylinder on a magnetic stirrer 103, turning on the stirrer for stirring, adjusting stirring time and magnetic force according to the screening condition of excrement, standing for 10 minutes, turning on a first switch 203, and placing supernate into a second cylinder 3;
(3) adding saturated saline water into the liquid flowing into the first horn 2 to 15cm from the second horn 3, placing on a magnetic stirrer 103, stirring for 5 minutes, standing for 10 minutes, turning on a second switch 302, and allowing the excessive liquid to flow into a third horn 4;
(4) adding saturated salt solution into the liquid flowing into the second cylinder 3 and the third cylinder 4 to 15cm, namely separated coccidian oocysts;
(5) the separated coccidian oocysts were poured into a centrifuge 5 for centrifugation: when in use, the device switch 503 is turned on for preheating for 5 minutes, separated eggs are subpackaged, weighed and balanced and then symmetrically placed into the rotor 507, a cover controller switch d is pressed on the control panel 505, the rotor 507 is placed on the rotor clamping seat 504 to be rotationally clamped after the top cover 506 is turned on, and the top cover 506 is turned off according to the cover controller switch d; then, operating the time controller a for setting time for 10 minutes, operating the speed controller b for 3500 minutes, pressing a start key c and a pause key c to start centrifugation, opening a top cover 506 after stopping, taking out the centrifuge tube, pouring out supernatant, adding proper water, symmetrically placing the centrifuge tube into a rotor 507 after weighing and balancing, pressing a cover controller switch d on a control panel 505, placing the rotor 507 on a rotor clamping seat 504 to be tightly rotated after opening the cover, closing the cover by pressing the cover controller switch d, operating the time controller a for setting time for 10 minutes, operating the speed controller b for 3500 minutes, pressing the start key c and the pause key c to start centrifugation, opening the top cover 506 after stopping, and taking out the centrifuge tube;
(6) opening a fourth cylinder cover 602 in the worm egg culture device 6, adding a proper amount of 2.5% potassium dichromate into the centrifuged worm eggs, uniformly mixing, pouring into a fourth cylinder 601, adding 2.5% potassium dichromate to a position of 2cm, and covering the fourth cylinder cover 602;
(7) meanwhile, the automatic oxygen adding device 7 is used for adding oxygen, the rotary cover 603 on the worm egg culture device 6 is opened, the pipeline 702 is inserted into the worm egg culture device 6 below the liquid level, the air volume switch 703 is opened, and the air volume is 1cm3Introducing into ovum culture device 6 at a flow rate of/min for 2-3 times per day for 5 min for 2-3 daysAfter the gas is finished, the gas quantity switch 703 of the oxygen adding device is closed, the rotary cover 603 of the culture device is covered, and the sporulated oocysts obtained by separation culture can be obtained for 2-3 days and used for experimental research.
This embodiment easy operation, the worm ovum separation effect is obvious, can separate at every turn and collect a large amount of worm ova for experimental study does benefit to the research and development and the screening of anti coccidian medicine, and the device material generally uses glass as leading simultaneously, and the disinfection is convenient, repeatedly usable has wide market perspective.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. An animal coccidian oocyst separation and culture device is characterized by comprising a magnetic stirrer (1), a first number cylinder (2), a second number cylinder (3), a third number cylinder (4), a centrifuge (5), an insect egg culture device (6) and an automatic oxygenation device (7), wherein the magnetic stirrer (1) comprises an object stage (101), a time controller (102), a magnetic stirrer (103), a first base (104) and magnetic beads (105), the object stage (101) is arranged on the first base (104), and the time controller (102) and the magnetic stirrer (103) are arranged on an operation panel of the first base (104); the horn (2) comprises a first horn cover (201), a first horn body (202) and a first switch (203), the first horn cover (201) is arranged at the top end of the first horn body (202), and the first switch (203) is installed at the bottom of the first horn body (202); the second horn (3) comprises a second barrel (301) and a second switch (302), and the second switch (302) is installed at the bottom of the second barrel (301); the third horn (4) comprises a third barrel (401) and a third switch (402), and the bottom of the third barrel (401) is provided with the third switch (402); the centrifuge (5) comprises a second base (501), a socket (502), a device switch (503), a rotor clamping seat (504), a control panel (505), a top cover (506), a rotor (507) and a main body (508), wherein the main body (508) is installed on the second base (501), the socket (502) and the device switch (503) are installed on the side edge of the main body (508), the control panel (505) is arranged on the main body (508), the rotor (507) is installed in the main body (508) through the rotor clamping seat (504), and the top cover (506) is arranged at the top end of the main body (508); the worm egg culture device (6) comprises a fourth cylinder body (601), a fourth cylinder cover (602) and a rotary cover (603), wherein the fourth cylinder cover (602) is arranged at the top end of the fourth cylinder body (601), and the rotary cover (603) is arranged on the fourth cylinder cover (602) in a rotatable manner; the automatic oxygen adding device (7) comprises a micro gas reservoir (701), a pipeline (702) and a gas quantity switch (703), wherein the pipeline (702) is connected to the micro gas reservoir (701), and the gas quantity switch (703) is installed on the pipeline (702).
2. The device for separating and culturing the oocysts of the animal coccidia as claimed in claim 1, wherein the first cover (201) in the first barrel (2) is a hollow cover with a diameter of 10cm, the first cover (201) is clamped on the top wall of the first barrel (202), the first barrel (202) is a glass barrel with a wall height of 20cm and a diameter of 10cm, the barrel wall of the first barrel (202) is provided with a scale with a unit of 1cm and a total of 20cm, the barrel wall 10cm away from the bottom of the first barrel (202) is provided with an opening, the opening is connected with the barrel wall and has a width of 2cm, and the first switch barrel wall (203) is arranged at a position with a distance of 1cm away from the opening.
3. The device for separating and culturing the oocysts of the animal coccidia as claimed in claim 1, wherein the second cylinder (301) in the second cylinder (3) is a glass cylinder with a wall height of 20cm and a diameter of 10cm, the cylinder wall of the second cylinder (301) is provided with a scale of 20cm in total by taking 1cm as a unit, the cylinder wall 10cm away from the bottom of the second cylinder (301) is provided with an opening, the opening is connected with the cylinder wall and has a width of 2cm, and the second switch (302) is arranged at a position of 1cm away from the cylinder wall.
4. The device for separating and culturing the oocysts of the animal coccidia as claimed in claim 1, wherein the third cylinder (401) in the third cylinder (4) is a glass cylinder with a wall height of 20cm and a diameter of 10cm, the cylinder wall of the third cylinder (401) is provided with a scale of 20cm in total by taking 1cm as a unit, the cylinder wall 10cm away from the bottom of the third cylinder (401) is provided with an opening, the opening is connected with the cylinder wall and has a width of 2cm, and the third switch (402) is arranged at a position with the opening 1cm away from the cylinder wall.
5. The device for separating and culturing the oocysts of the animal coccidia as claimed in claim 1, wherein the main body (508) of the centrifuge (5) is a stainless steel cuboid, and a time controller (a), a rotating speed controller (b), a start and pause key (c) and a cover controller switch (d) are arranged on a control panel (505); the rotor (507) is a conical rotor with a large upper part and a small lower part, 12 centrifugal grooves are formed in the rotor (507), and clamping grooves (509) which are in sawtooth-shaped fit with the rotor clamping seats (504) are formed in the bottom end of the rotor (507).
6. The device for separating and culturing the animal coccidian oocyst as claimed in claim 1, wherein a fourth cylinder (601) in the device (6) for culturing the animal coccidian oocyst is a glass cylinder with a wall height of 5cm and a diameter of 10cm, the cylinder wall of the fourth cylinder (601) is provided with a scale with a unit of 1cm, and the fourth cylinder cover (602) is made of stainless steel.
7. The device for separating and culturing the oocysts of the animal coccidia as claimed in claim 1, wherein the micro gas reservoir (701) in the automatic oxygenation device (7) is a stainless steel cylinder gas reservoir with the diameter of 10cm and the height of 20cm, the pipeline (702) is a plastic pipeline, and an adjustable gas amount switch (703) is arranged on the pipeline (702) at a position 12cm away from the gas reservoir.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011580595.4A CN112760211A (en) | 2020-12-28 | 2020-12-28 | Animal coccidian oocyst separation and culture device |
Applications Claiming Priority (1)
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