CN112715349B - A Breeding Method of Perennial Rice Glycosterile Line - Google Patents
A Breeding Method of Perennial Rice Glycosterile Line Download PDFInfo
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- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
本发明公开一种多年生型水稻雌性核不育系的选育方法，具体为：选用江西东乡普通野生稻作母本，用水稻雌性核不育突变体作父本进行杂交，后代通过自交、聚合杂交、回交等方法，结合江西东乡普通野生稻宿根再生特性及水稻雌性不育基因FST分子鉴定方法，通过保留fst基因杂合群体株系的方法加代选育，直至多年生雌性核不育分离株系世代高于F8以上时，即获得稳定的多年生型雌性核不育系及其可育近等基因系。所选育的水稻雌性核不育系具有多年生特性和fst基因，可进行无性繁殖，解决了雌性核不育水稻繁殖问题，实现了轻简化栽培，生产效率高；多年生型雌性核不育系100％败育，花粉可育，可作为杂交水稻理想的花粉供体，实现全机械化制种。
The invention discloses a breeding method for a perennial rice female sterility line. Specifically, the method comprises the following steps: selecting Jiangxi Dongxiang common wild rice as the female parent, using the rice female sterility mutant as the male parent for crossbreeding, and the offspring are subjected to self-crossing, Aggregate hybridization, backcrossing and other methods, combined with the perennial regeneration characteristics of common wild rice in Dongxiang, Jiangxi Province and the molecular identification method of FST gene of female sterility gene in rice, through the method of retaining the fst gene heterozygous population line and breeding until the separation of perennial female sterility When the line generation is higher than F 8 or more, a stable perennial female genital sterile line and its fertile near-isogenic line are obtained. The selected rice GMS line has perennial characteristics and fst gene, and can be reproduced asexually, which solves the problem of GMS rice reproduction, realizes light and simplified cultivation, and has high production efficiency; the perennial GMS line 100 % abortion, the pollen is fertile, and it can be used as an ideal pollen donor for hybrid rice to realize fully mechanized seed production.
Description
Technical Field
The invention relates to a method for breeding a female genic male sterile line of perennial rice, belonging to the technical field of rice genetic breeding.
Technical Field
Modern society, the ever-increasing conflict of population and food needs with problems of climate change, resource scarcity, low land utilization, etc., traditional annual crop planting systems subject the agricultural ecosystem relied on by humans to be survived to unsustainable, biodiversity faces serious crises, and it will become increasingly difficult to meet the production needs of human society in the future (hypericum, long-flesh, li-gathering, etc. multiannual crop research progress [ J ]. chinese agronomic report, 2013,29(18): 16-20.; Rasche F, bladodataya E, emmering C, et al. Compared with annual crops, perennial crops are under worldwide attention with advantages such as long growing season, developed root system, strong stress resistance, high soil utilization rate And simple cultivation management (Godfray H C J, Beddington J R, crack I R, et al Food Security: The change of Feeding 9Billion Peap [ J ], Science,2010(327): 812; Timman D, Socolow I L, Foley J A, et al Beneficial biological of The Food, energy, And vision triangle [ J ]. Science, (325): 270: 271; Jer D G, John P R. personal soil Security for The root [ R ] environmental research, In, Technology, 2009: 42). At present, the creation of perennial crops mainly adopts two ways of directly domesticating wild species or distant hybridization between annual cultivated species and near-source wild species.
The ordinary wild rice (Oryza. rufipogon Griff) distributed in the northwest of the Yangxi province is the ordinary wild rice distributed in the northwest of the world so far, the plants in the natural population of the ordinary wild rice completely depend on dormant buds for asexual propagation, the regeneration capability is strong, the ordinary wild rice is shown as the characteristics of perennial ordinary wild rice, and the ordinary wild rice has strong cold resistance, drought resistance, disease and insect resistance (break, survey of morphological character variation in the natural population of the east and rural wild rice [ J ]. China Rice science, 1996(4) ], Chengdai Dazhou, Zhang Bake, Xiuwenhua and the like [ J ]. Jiangxi agricultural science, 2008,20(12):12-16 ]. Under natural conditions, ordinary wild rice plants in east and rural areas of the west and the west can tolerate low temperature overwintering survival at-8.5 ℃ and germinate in the spring of the next year (how light is stored, Shu and Hui, Monday is strong, etc. the observation of overwintering performance of the wild rice in east and rural areas of Wuhan university (science edition), 1996(2): 252-.
The domestic scholars make more researches on the cold resistance of the common wild rice in the east and the rural areas of the west. For example, the cold-resistant regeneration japonica rice variety is bred by utilizing the cold-resistant overwintering characteristic, such as Dongyou I (Chen Dazhou, Shaoye green, Piyonghua, and the like. preliminary genetic improvement on cold resistance of Dongxiang wild rice [ J ] academic newspaper of Jiangxi agriculture university, 2003,25(1): 8-11.; breeding of Chen Dazhou, Shaoye green, Piyonghua, and the like. overwintering japonica rice variety Dongyou I [ J ] crop research, 2007,21 (3)). The researchers also performed QTL mapping on their overwintering (overwintering) trait (Liang Y, Zheng J, Yan C, et al. locating QTLs controlling overwintering training trail in Chinese permanent farming world [ J ]. MGG Molecular & General Genetics,2017,293(392): 1-13.).
At present, the research of directly utilizing the perennial root property of common wild rice in east and rural areas of the west is less, in the invention patent with the application number of CN201010283755.9 'a natural wintering rice stump of perennial rice and a method for identifying the activity of the wintering rice stump', the research institute of the agricultural academy of the west and the west proposes that the cultivated rice and the strong cold-resistant Dongxiang wild rice are used for sexual hybridization and backcross, the progeny thereof overwinter under the natural stump condition, the next spring and the dormant bud of the rice stump germinate into seedlings, and the viability of the rice stump is identified to further breed the perennial cold-resistant progeny. The method adopts stump-remained overwintering screening, requires identification after dormant buds germinate in spring of the next year, and has a long execution period.
Rice Female Sterility natural mutant (FST) Female organ is completely aborted, due to the frame shift mutation caused by the deletion of 8bp base In the MADS-box region of FST gene, ovule dysplasia and complete abortion of zygotic embryo and endosperm are caused, although male organ is normally developed, anther is normally cracked and pollen is scattered, pollen quantity is sufficient, pollen fertility rate is more than 90%, but sexual reproduction can not be carried out by obtaining seeds (Lee D., Chen L J., Ha W G.et al.Single Recessed Genetic Female Sterility In Rice.International Rice Research Notes,2002,27: 22-23.; Lee DS., Chen LJ., Li CY., et al.the BsisterMADS Gene FST definitions Ovule Patterning and Development of the Zygotic Embryo and Endosperm. PLoS ONE,2013,8(3): e 58748.). The breeding method of female sterile rice is a great obstacle to the breeding and utilization of female sterile rice, and the early stage of research proposes to solve the problem by using a genetic engineering method, such as that disclosed in application No. CN201410116096.8The invention discloses a method for mechanically producing seeds of hybrid rice by utilizing female sterility, which is used for producing female sterile rice seeds by utilizing a method of combining transgene complementation with fluorescence sorting.
In the existing methods and technologies, the perennial and perennial characteristics of the common wild rice in east and rural areas of Jiangxi are rarely utilized, and meanwhile, the existing female sterile rice propagation technology is complex, limited in purity and large in practical application limitation. If the strong perennial root and the perennial characteristic of the common wild rice in the east village can be effectively utilized, the creation of the perennial rice female genic male sterile line is necessary.
Disclosure of Invention
The invention provides a breeding method of a multiannual female genic male sterile line of rice, which aims to solve the technical problem of breeding of the female genic male sterile line of rice by fully utilizing the regeneration characteristic of perennial roots of common wild rice in east and rural areas of the west. The perennial character of wild rice in east and west of Jiangxi is introduced into female genic sterile rice through a hybridization method, and the excellent characteristics of the cultivated rice are polymerized, so that the female genic sterile line of the rice which has the perennial character and can be subjected to asexual reproduction is cultivated, and the simplified cultivation can be realized in field production. Meanwhile, the female genic male sterile line of the perennial rice is completely fruitless, can be used as an ideal pollen donor in the production of hybrid rice seeds, does not need to harvest the male parent singly, is favorable for mechanical seed production, improves the seed purity, and lays a good foundation for promoting the innovative development of a heterosis utilization technical system. In order to solve the technical problems and achieve the purposes, the invention is realized by the following technical scheme:
a method for breeding a female genic male sterile line of a multi-year rice comprises the following steps:
(1) selecting common wild rice in east countryside of Jiangxi as a female parent, and using a rice female nuclear sterile mutant as a male parent to perform hybridization;
(2) subjecting the single cross progeny F obtained in the step (1) to1Selfing to obtain F2A population;
(3) combining the phenotypic identification of perennial habit and the identification of FST molecule of female sterile gene of rice to assist selection, F is described in the step (2) above2Selecting female genic sterile single plants with perennial habit from the segregation population as male parents, combining breeding targets, and performing polymerization hybridization with other excellent rice varieties or lines to obtain a first polymerization hybridization F1A population;
(4) the first polymeric hybridization F described in the step (3)1In the population, the method of combining the phenotype identification of perennial habit and utilizing the FST molecular identification of rice female sterile gene is used for assisting in selecting the obtained polymeric hybrid filial generation F with female genic male sterile gene heterozygosis1Selfing to obtain the first polymerized cross F2Population, hybridizing F from the first aggregate2Selecting plants in the separated population, and planting according to the plant lines to obtain a first polymeric hybrid F2Separating each strain group of the groups;
or (5) the first convergent cross F described in step (3) in combination with the breeding objective1In the population, the method combining the phenotype identification of the perennial habit and the FST molecular identification of the rice female sterile gene is used for assisting in selecting the obtained polymeric hybrid filial generation F with female nuclear sterile gene heterozygosis1As male parent, and then carrying out polymerization hybridization with other excellent rice lines or combination, and so on, from the final F2Selecting plants from the filial generation and planting according to the plant line to obtain the final polymerized filial F2Separating each strain group of the groups;
(6) the first polymeric hybridization F from the step (4)2Segregating the individual line populations of the population or the final aggregate hybrid F described in step (5)2In each strain group of the segregating group, by combining breeding targets and adopting perennial habit and female nuclear sterile abortion phenotype identification, selecting female nuclear sterile fertility segregating strains with perennial habit, selecting more than 5 fertile single plants from each fertility segregating strain, and planting 50-100 plants according to strains for selfing and generation adding;
(7) planting 50-100 self-bred generation-added strains according to the strains in the step (6), combining breeding targets, adopting perennial habit and female nucleic sterility abortion phenotype identification, and continuously selecting female nucleic with perennial habitSterile fertility segregating line, selecting more than 5 fertile single plants from each fertility segregating group, planting 50-100 plants according to line for selfing and generation-adding until generation-adding to F8And screening to obtain the perennial female sterile line with stable perennial habit and female sterile abortion character and/or the near isogenic line thereof by combining the identification of the perennial habit and the female sterile abortion phenotype.
Further, the multi-year female sterile male sterile line comprises a fst gene mutation site.
Further, the rice female sterile mutant in the step (1) is G39.
The invention also provides the application of the breeding method of the perennial rice female genic male sterile line in further breeding rice varieties.
Compared with the prior art, the invention has the beneficial effects that:
1. the common wild rice in east and rural areas of Jiangxi is of a perennial type, has strong cold resistance, has the perennial characteristic that dormant buds overwinter and germinate again to form seedlings in the next year, is transferred into female nuclear sterile line rice by using crossbreeding and polymerization breeding methods to breed the female nuclear sterile line of the rice with perennial root, overyear regeneration and other perennial habits, and can solve the propagation problem of the female nuclear sterile line by a asexual propagation means.
2. In the process of breeding the female genic male sterile line of the perennial rice, the mature period of the filial generation can observe new tillering at the base of the homozygote female genic male sterile plant, namely the perennial habit is shown, which is beneficial to directly screening the characters; meanwhile, the rice stump is kept in the field, and the dormant bud of the rice stump is regenerated over season through conventional fertilizer and water management.
3. The female genic male sterile line of the perennial rice bred by the invention has 100 percent abortion of female organs, 0 natural selfing and/or outcrossing maturing rate and sufficient and normal fertility of pollen, can be used as an ideal pollen donor in the production of hybrid rice seeds because the female genic male sterile line is completely untruncated, does not need a single-harvest male parent, is favorable for mechanical seed production, improves the purity of the seeds and lays a good foundation for promoting the innovative development of a heterosis utilization technology system.
4. The cultivated perennial rice female genic male sterile line can be popularized and applied in south-east subtropical and subtropical regions, and can be used for one-time seedling planting and multi-season use by keeping piles in a hybrid rice seed production field by utilizing the ratoon regeneration characteristic, thereby saving the production cost and improving the production efficiency.
Drawings
FIG. 1: perennial root regeneration characteristic of perennial female sterile line. Wherein A is the plant type of the multiannual female sterile genic line Yunling 301FS in the mature period, a: a local enlargement of new tillers in the mature stage of Yunling 301 FS; b, plant type of the multiannual female sterile nuclear line Yunling 314FS in mature period, B: an enlarged view of a part of new tillers in the mature stage of Yunling 301 FS.
FIG. 2: the perennial female genic male sterile line is shown by the overyear regeneration and growth. C, growing in the Yunling 301FS field for overyear regeneration; and D, the Yunling 314FS field grows more year by year.
FIG. 3: the cloud 301FS spectrogram.
FIG. 4: the cloud 314FS family.
Detailed Description
The terms:
perennial or perennial habits: the hybrid rice is characterized in that the hybrid breeding of the common wild rice in the east and rural areas of the west and the west or the hybrid progeny of the common wild rice and the cultivated rice has strong perennial property, and can still generate new tillering and rice stumps can be regenerated for years more than year in the mature period (figure 1).
FST gene: wild-type FST gene, full-name English, FEMALE-STERILE, GeneBank accession number: DQ 004266.
Female nuclear sterile gene fst or fst gene: mutants of the FST gene are indicated in lower case letters.
Female genic sterility or female genic sterility lines: refers to a plant or a plant line of which the filial generation has fst gene mutation sites and the genotype is homozygous (-/-); and the corresponding near-allelic line is a female fertile line, which is heterozygous or homozygous in genotype (-/+ or +/+).
Perennial female genic male sterile line: the female genic male sterile line is a female genic male sterile line which still generates new tillers in the mature period and has perennial regeneration habits and fst genes of perennial roots of common wild rice in east and rural areas of the west.
The following experimental methods are conventional in the art unless otherwise specified, and the reagents and rice materials used therefor are commercially available or according to national regulations.
The Kunming greenhouse is a greenhouse of the Yunnan agriculture university rice research institute in Kunming city, Yunnan province. The test was conducted in Kunming City, Yuanjiang county, Yuanyang county, and Chuxiong Yi nationality, autonomous, Lufeng county, of Yunnan province.
And (4) conjugation 41: the japonica rice variety (Liuji Xin, Zhao national treasure, Jianjiang province, etc.. the breeding of the Guangdong high-yield cold-resistant disease-resistant japonica rice new variety hybrid No. 41 [ J ]. the southwest agricultural science, 2002,15(004):5-9.) can be purchased through commercial channels (sold in the market).
The following rice materials are disclosed in the following non-patent documents, and the applicants have a preserved, available, contact address: the FengYuan route 452 in the area of the dragon, Yunnan province, Yunnan agricultural university (noted: Yunnan agricultural university institute of rice farming), zip code: 650201.
hwaseonchalbyo: high Quality japonica Rice Variety (Moon, H.P., Cho, S.Y., Kim, H.Y., et al.an other-derived Disease Resistance and High Quality Glutinous Rice Variety "Hwaseon charbye" [ J ]. RDA J ounal of agricultural science, 1993).
Ansanbyeo: high quality japonica Rice variety (Choi, H.C., Kim, Y.K., Ahn, S.N., et al.A. new Low-resistant and High-yielding Rice operable adaptive to Direct feeding "Ansanbyeo" [ J ]. rda journal of crop science, 1997.).
G39: female nuclear sterility natural mutants (Lee, D.S., Chen, L.J., Li, C.Y., et al.the Bsister MADS Gene FST determinines Ovule Patterning and Development of the Zygotic Embryo and Endosperm [ J ]. PLoS ONE,2013,8(3), 26-33).
AWG, intermediate strain of japonica rice breeding, also known as AWG-cynyn. (Chen, L.J., Lee, D.S., Tan, X.L., et al, Characterization of Female-parent Rice and its Great Point for Innovation of selected Production Technology of Hybrid Rice, International Symposium on "100 years of Rice Science and Looking Beyond", India, 2012).
CH8, also known as Sanghaehyangheolua: japonica rice variety (Nadir, S., Li, W., Zhu, Q., et al. A novel discovery of a long term repeat retrotransposon-induced hybrid rice in rice [ J ]. Journal of Experimental Botany,2019,70(4), 1197. sup. 1207.).
Gayabyeo: indica intermediate varieties (Jeon, Y., Ahn, S., Choi, H.et al.identification of a RAPD marker linked to a brown plant tolerance gene in rice [ J ]. Euphytoica, 1999,107, 23-28.).
The method for identifying FST molecules of rice female sterile genes described in the following examples assists selection (denoted MAS in the relevant figures, namely Marker-assisted selection, MAS): the invention is a method for identifying paddy female sterile gene FST molecules disclosed in patent document with patent number CN101654703B applied by the applicant, and wild type (+/+), mutant (-/-), and heterozygous (+/-) paddy plants of the female sterile gene FST can be obtained accurately, quickly and efficiently by screening specific primers of the FST gene. The method for identifying the rice female sterile gene FST Molecule Assisted Selection (MAS) specifically comprises the following steps: taking leaves of rice material to be identified, extracting total DNA of the rice, and using four specific primers of female nuclear sterile gene FST: performing PCR amplification on the total DNA of the rice by using a Gf1 primer, a Gr1 primer, a Jf1 primer and a Jr1 primer, adding 3 mu l of loading buffer after PCR reaction, uniformly mixing and loading 6 mu l of the mixture, and performing electrophoresis for 40min by using 2% agarose gel containing 0.1 mu g/ml of EtBr and 1 XTAE buffer and 125V; and (5) detecting on an ultraviolet transilluminator. The total volume of the amplification system was 15. mu.l, wherein ddH was added2O11.24. mu.l, containing Mg2+10 XBuffer 1.5. mu.l, 2.5mM dNTP 1.2. mu.l, 4 primer concentrations each 50 pmol/. mu.l each 0.12. mu.l, 5U/. mu.l Takara Taq HS 0.08. mu.l, 100 ng/. mu.l template DNA 0.5. mu.l. The amplification program is 94 ℃ for 5 min; 30 cycles of 94 ℃ for 30s, 58 ℃ for 30s and 72 ℃ for 1 min; 5min at 72 ℃. The PCR product size of FST homozygous wild type (+/+) of rice female sterile gene is 395bp and 663bp, the PCR product size of FST homozygous mutant (-/-) is 298bp and 655bp, the PCR product size of mutant type female nuclear sterile material heterozygous (+/-) is 298bp, 395bp and 663bp or 298bp and 3bp95bp and 655 bp.
The sequence of the Gf1 primer is shown as SEQ ID NO: 1, the sequence of the Gr1 primer is shown as SEQ ID NO: 2, the sequence of the Jf1 primer is shown as SEQ ID NO: 3, the sequence of the Jr1 primer is shown as SEQ ID NO: 4, respectively.
The present invention is further illustrated by the following detailed description, which is given by way of example only and not by way of limitation, and various modifications or improvements can be made by those skilled in the art based on the embodiments of the present invention without departing from the basic idea of the invention.
Example 1: breeding method of perennial rice female genic male sterile line Yunling 301FS
(1) In 2006 Kunming greenhouse, using Dongxiang wild rice (DXWR) as female parent and using female nuclear sterile natural mutant G39 as male parent to make hybridization to obtain single-cross progeny F1And (4) hybridizing.
(2) Single cross generation F is planted in Kunming greenhouse in 20071Obtaining F2And (4) separating the populations.
(3)2008, Kunming was planted with hybrid F2Segregating the population, screening out female genic male sterile lines F with perennial habit by phenotype identification of perennial habit and auxiliary selection (MAS) method of rice female sterile gene FST molecular identification216 as male parent, combining with breeding target, and then using other rice fine variety hybrid 41 (japonica rice variety) as female parent to make double crossing (polymerization hybridization).
(4) Double-cross progeny F1 is planted in Kunming in 2009 and is reused with breeding intermediate strain (Hwaseonchalbyo/Ansanbyo) F obtained in the early stage5Performing triple crossing (cross-breeding) as female parent to obtain triple-crossing filial generation F1And (4) hybridizing.
(5) Planting three-way filial generation F in Kunming in 20101The hybrid is characterized in that the phenotype identification of perennial habit is combined, the auxiliary selection (MAS) method of rice female sterile gene FST molecular identification is used for screening heterozygous plants as male parents, and the japonica rice variety Ansan is usedCarrying out polymerization hybridization by taking byeo as a female parent;
(6) from 2012, the method comprises the steps of planting polymerized filial generation in the early season/late season of Yuanjiang county and Yuanyang county, screening female nuclear sterile separate lines with perennial habit by combining perennial habit and female sterile abortion phenotype identification, selecting more than 5 fertile single plants from each fertility separate group in each generation, continuously planting 50-100 plants according to lines for selfing and generation-adding, and selfing the heterozygous lines to F in 201711In generations, the perennial female genic male sterile line and its near allelic line have completely stable properties, and are bred and named as Yunling 301 FS.
The Yunling 301FS sterile line has moderate plant height, the average plant height is 104.7cm, the average spike length is 26.6cm, no spike is covered, the average grain number of single spike is 266, short top awn exists, the average tillering number of single plant is about 30, the tillering force is strong, the continuous overyear regeneration is good, the average effective spike number of single plant is about 29, the sword leaf is straight, the leaf color is bright green, the stem is strong, and the field shows rice blast resistance and bacterial blight resistance. All plants in the flowering period have normal male organ development, large anther, bright yellow color, normal cracking and pollen scattering, sufficient pollen quantity and average pollen fertility rate of 93.8 percent; the rice ears in the mature period are completely sterile, the natural selfing/outcrossing seed setting rate is 0, and the sterile plant rate and the sterility degree reach 100 percent. The rice grain of the near isogenic line is short, round and transparent, and has the average direct starch content of 15.6 percent, the protein content of 9.5 percent and better rice quality.
The family map of Yunling 301FS is shown in FIG. 3.
Example 2: breeding method of perennial rice female genic male sterile line Yunling 314FS
(1) In 2006 Kunming greenhouse, using Dongxiang wild rice (DXWR) as female parent and using female nuclear sterile natural mutant G39 as male parent to make hybridization to obtain single-cross progeny F1And (4) hybridizing.
(2) Single cross generation F is planted in Kunming greenhouse in 20071Obtaining F2And (4) separating the populations.
(3)2008, Kunming was planted with hybrid F2Segregating the population, screening for perennial phenotype identification and FST molecular identification of rice female sterile geneHabit-producing female genic male sterile line F216 as male parent, combining with breeding target, and double crossing with other rice fine variety hybrid line 41 (japonica rice variety) as female parent to obtain double crossing progeny F1And (4) hybridizing.
(4) Double-cross progeny F planted in Kunming in 20091Then carrying out triple crossing by using Ansanbyeo as a female parent; three-way cross breeding is planted in Kunming in 2010, a hybrid plant is screened out by using a method of paddy female sterile gene FST molecular identification for assisting selection (MAS), another stable line (CH8/Gayabyeo// AWG) with excellent characters is used as a female parent for polymerization and hybridization, and hybrid progeny is obtained in 2011.
(5) Continuously planting polymerized filial generation in the positive season of Kunming city, Yuanjiang county and Yuanyang county in the early season/late season from 2011, screening female nuclear sterile fertile plant segregating strains with perennial habits by combining phenotype identification of perennial habits and female sterile abortion phenotype identification, selecting 7-10 fertile single plants from each fertility segregating population by each generation, continuously planting 60-100 selfing generations according to strains, and selfing until the heterozygous strains are F in 201711In generations, the perennial female genic male sterile line and its near allelic line have completely stable properties, and are bred and named as Yunling 314 FS.
The sterile line of Yunling 314FS has stable sterility, female sterile plants are transplanted in rice stakes in fields of Kunming city and Lufeng county in 2017, and natural propagation inspection results show that: all plants in the flowering period have normal male organ development, large anther, bright yellow color, normal cracking and pollen scattering, sufficient pollen quantity and average pollen fertility rate of 96.8 percent; the rice ears in the mature period are completely sterile, the natural selfing/outcrossing seed setting rate is 0, and the sterile plant rate and the sterility degree reach 100 percent.
The Yunling 314FS sterile line has moderate plant height, the average plant height of 92.7cm, the average spike length of 24.7cm, no covering ears, the average number of grains of a single spike of 174, the average number of effective spikes of a single plant of about 13, short top awns, the average number of tillers of a single plant of about 26, strong tillering force, good continuous overyear regeneration, straight sword leaves, bright green leaf color, strong stems, and rice blast and bacterial blight resistance in the field; the rice grain of the near isogenic line is short, round and transparent, and has the average direct starch content of 15.6 percent, the protein content of 9.5 percent and better rice quality.
The above embodiments are only for illustrating the technical solutions of the present invention, and are only preferred embodiments of the present invention, and are not intended to limit the present invention; modifications, equivalents, improvements and the like of the technical solutions described in the foregoing embodiments by those skilled in the art within the spirit and principle of the present invention should be included in the protection scope of the present invention.
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| CN1298450A (en) * | 1998-02-20 | 2001-06-06 | 曾尼卡有限公司 | Hybrid seed production |
| CN101658129A (en) * | 2009-09-18 | 2010-03-03 | 云南农业大学 | Method of female sterile gene FST for hybrid rice breeding |
| CN101697706A (en) * | 2009-11-02 | 2010-04-28 | 湖南杂交水稻研究中心 | Application and cultivation method of high-frequency recessive female sterile rice restorer lines |
| WO2011090752A1 (en) * | 2009-12-29 | 2011-07-28 | The Board Of Governors For Higher Education, State Of Rhode Island And Providence Plantations | Male and female sterility lines used to make hybrids in genetically modified plants |
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|---|---|---|---|---|
| CN1298450A (en) * | 1998-02-20 | 2001-06-06 | 曾尼卡有限公司 | Hybrid seed production |
| CN101658129A (en) * | 2009-09-18 | 2010-03-03 | 云南农业大学 | Method of female sterile gene FST for hybrid rice breeding |
| CN101697706A (en) * | 2009-11-02 | 2010-04-28 | 湖南杂交水稻研究中心 | Application and cultivation method of high-frequency recessive female sterile rice restorer lines |
| WO2011090752A1 (en) * | 2009-12-29 | 2011-07-28 | The Board Of Governors For Higher Education, State Of Rhode Island And Providence Plantations | Male and female sterility lines used to make hybrids in genetically modified plants |
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