CN112708592A - 3D structure of osteoblast protein - Google Patents
3D structure of osteoblast protein Download PDFInfo
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- CN112708592A CN112708592A CN202110037018.9A CN202110037018A CN112708592A CN 112708592 A CN112708592 A CN 112708592A CN 202110037018 A CN202110037018 A CN 202110037018A CN 112708592 A CN112708592 A CN 112708592A
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- osteoblast
- osteoblasts
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- protein
- somatic cells
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- 210000000963 osteoblast Anatomy 0.000 title claims abstract description 62
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 30
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 9
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 8
- 241000124008 Mammalia Species 0.000 claims abstract description 7
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 210000004409 osteocyte Anatomy 0.000 claims abstract description 4
- 230000008672 reprogramming Effects 0.000 claims abstract description 4
- 108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 claims description 6
- 102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 claims description 6
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 6
- 230000004072 osteoblast differentiation Effects 0.000 claims description 5
- 108010026552 Proteome Proteins 0.000 claims description 3
- 159000000007 calcium salts Chemical group 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 claims description 3
- 210000002950 fibroblast Anatomy 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000010186 staining Methods 0.000 claims description 3
- 238000000539 two dimensional gel electrophoresis Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000010276 construction Methods 0.000 abstract description 10
- 208000006735 Periostitis Diseases 0.000 description 1
- 101800001554 RNA-directed RNA polymerase Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000014461 bone development Effects 0.000 description 1
- 230000008468 bone growth Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 108010010201 core binding factor alpha Proteins 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 101150044508 key gene Proteins 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 210000003460 periosteum Anatomy 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Disclosed is a 3D structure of osteoblast protein, the 3D structure of which is formed by combining osteoblasts prepared by introducing reprogramming-related genes or expression products thereof into somatic cells, which are derived from somatic cells of mammals, with inorganic components to form bone, the osteoblasts being embedded in a matrix to be differentiated into osteocytes. According to the 3D structure of the osteoblast protein, the organic components and the inorganic components generated by osteoblasts are combined to form a bone, the construction process is simple, and the construction efficiency is higher.
Description
Technical Field
The invention relates to the technical field of osteoblasts, in particular to a 3D structure of osteoblast protein.
Background
Osteoblasts are mainly differentiated from mesenchymal progenitor cells in the stroma of the internal and external periosteum and bone marrow, and can specifically secrete a variety of bioactive substances, regulate and influence the formation and reconstruction processes of bones.
The existing osteoblast protein 3D structure has the problems of complex construction process and low construction efficiency.
Disclosure of Invention
The invention aims to provide a 3D structure of osteoblast protein, which aims to solve the problems of complex construction process and low construction efficiency of the existing 3D structure of osteoblast protein in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a 3D structure of osteoblast protein, the 3D structure of which is formed by combining osteoblasts prepared by introducing reprogramming-related genes or expression products thereof into somatic cells, the osteoblasts being derived from somatic cells of mammals, and inorganic components combined to form bone, the osteoblasts being embedded in a matrix to be differentiated into osteocytes.
Preferably, the osteoblast active state is spindle, cone or cube shaped.
Preferably, the osteoblast differentiation process is affected by a nuclear binding factor, which is specifically expressed by osteoblasts.
Preferably, the inorganic component comprises an inorganic salt, the inorganic salt being a calcium salt.
Preferably, the inorganic component further comprises water.
Preferably, the osteoblast protein is differentially expressed as follows: subculturing MG63 osteoblasts, randomly dividing into CGRP group and control group, extracting protein in each osteoblast, performing two-dimensional electrophoresis separation, staining with Coomassie brilliant blue, and scanning to analyze protein expression change.
Preferably, the result of the differential expression of the osteoblast protein is as follows: after CGRP acts on osteoblasts, the proteome of the osteoblasts is changed remarkably.
Preferably, the somatic cells of the mammal include fibroblasts or gingival cells.
Compared with the prior art, the invention has the following beneficial effects: according to the 3D structure of the osteoblast protein, the organic components and the inorganic components generated by osteoblasts are combined to form a bone, the construction process is simple, and the construction efficiency is higher.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely in conjunction with the implementation of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: A3D structure of osteoblast protein, its 3D structure is formed by osteoblast and inorganic component combination, osteoblast is prepared by introducing the relevant gene of reprogramming or its expression product into somatic cell, osteoblast it is derived from somatic cell of mammal, organic composition that produce by osteoblast combine with inorganic component to form the bone, osteoblast bury in the matrix and differentiate into osteocyte, osteoblast active state is shuttle, taper or cube, osteoblast differentiation process is influenced by the binding factor of the nucleus, the binding factor of the nucleus is expressed by osteoblast specificity, the inorganic component includes inorganic salt, the inorganic salt is calcium salt, the inorganic component also includes water, organic composition that produce by osteoblast combines with inorganic component to form the bone, the construction process is simpler, and the construction efficiency is higher, the binding factor-alpha 1 of the nucleus is expressed by osteoblast specificity, is a factor determining osteoblast differentiation, the osteoblast differentiation pathway regulated by the factor is irreplaceable, CBF-alpha 1 is a key gene for bone formation, determines the generation and differentiation of osteoblasts, and plays an important role in maintaining normal bone growth and development, and osteoblast proteins are differentially expressed as follows: subculturing MG63 osteoblasts, randomly dividing into CGRP group and control group, extracting proteins in each osteoblast, performing two-dimensional electrophoresis separation, performing Coomassie brilliant blue staining, and scanning to analyze protein expression change, wherein the difference expression result of osteoblast protein is as follows: after CGRP acts on osteoblasts, the proteome of the osteoblasts is obviously changed, and the somatic cells of mammals comprise fibroblasts or gingival cells.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A 3D structure of osteoblast protein, wherein the 3D structure is formed by combining osteoblasts prepared by introducing reprogramming-related genes or expression products thereof into somatic cells, which are derived from somatic cells of mammals, with inorganic components to form bone, and the osteoblasts are embedded in a matrix to be differentiated into osteocytes.
2. 3D structure of osteoblast protein according to claim 1, characterised in that: the osteoblast active state is spindle, cone or cube shaped.
3. 3D structure of osteoblast protein according to claim 1, characterised in that: the osteoblast differentiation process is influenced by nuclear binding factors, which are specifically expressed by osteoblasts.
4. 3D structure of osteoblast protein according to claim 1, characterised in that: the inorganic component comprises an inorganic salt, and the inorganic salt is a calcium salt.
5. 3D structure of osteoblast protein according to claim 1, characterised in that: the inorganic component also includes water.
6. 3D structure of osteoblast protein according to claim 1, characterised in that: the differential expression of the osteoblast proteins was as follows: subculturing MG63 osteoblasts, randomly dividing into CGRP group and control group, extracting protein in each osteoblast, performing two-dimensional electrophoresis separation, staining with Coomassie brilliant blue, and scanning to analyze protein expression change.
7. A3D structure of osteoblast protein according to claim 6, characterised in that: the results of differential expression of the osteoblast protein were as follows: after CGRP acts on osteoblasts, the proteome of the osteoblasts is changed remarkably.
8. 3D structure of osteoblast protein according to claim 1, characterised in that: the somatic cells of the mammal include fibroblasts or gingival cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110037018.9A CN112708592A (en) | 2021-01-12 | 2021-01-12 | 3D structure of osteoblast protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110037018.9A CN112708592A (en) | 2021-01-12 | 2021-01-12 | 3D structure of osteoblast protein |
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| Publication Number | Publication Date |
|---|---|
| CN112708592A true CN112708592A (en) | 2021-04-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110037018.9A Pending CN112708592A (en) | 2021-01-12 | 2021-01-12 | 3D structure of osteoblast protein |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101227915A (en) * | 2005-06-13 | 2008-07-23 | 世源世龙股份有限公司 | Bone formation composition composed of mixture of osteoblast and bio-matrix and its manufacturing method |
| CN105555955A (en) * | 2013-07-26 | 2016-05-04 | 京都府公立大学法人 | Osteoblast and method for preparing same |
| CN108350433A (en) * | 2015-08-21 | 2018-07-31 | 京都府公立大学法人 | Osteoblast and preparation method thereof |
-
2021
- 2021-01-12 CN CN202110037018.9A patent/CN112708592A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101227915A (en) * | 2005-06-13 | 2008-07-23 | 世源世龙股份有限公司 | Bone formation composition composed of mixture of osteoblast and bio-matrix and its manufacturing method |
| CN105555955A (en) * | 2013-07-26 | 2016-05-04 | 京都府公立大学法人 | Osteoblast and method for preparing same |
| CN108350433A (en) * | 2015-08-21 | 2018-07-31 | 京都府公立大学法人 | Osteoblast and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 吴凡等: "成骨细胞接种于珊瑚-羟基磷灰石构建骨组织的研究", 《解放军医学杂志》 * |
| 吴梦等: "CGRP作用下的成骨细胞蛋白质差异表达", 《实用口腔医学杂志》 * |
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Application publication date: 20210427 |