CN112694516A - Polypeptide capable of reducing sensitization of beta lactoglobulin in cow milk - Google Patents
Polypeptide capable of reducing sensitization of beta lactoglobulin in cow milk Download PDFInfo
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- CN112694516A CN112694516A CN202011434624.6A CN202011434624A CN112694516A CN 112694516 A CN112694516 A CN 112694516A CN 202011434624 A CN202011434624 A CN 202011434624A CN 112694516 A CN112694516 A CN 112694516A
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- Prior art keywords
- lactoglobulin
- sensitization
- beta lactoglobulin
- polypeptide capable
- terminal
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- 108010060630 Lactoglobulins Proteins 0.000 title claims abstract description 16
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 16
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 10
- 206010070834 Sensitisation Diseases 0.000 title claims abstract description 9
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 9
- 230000008313 sensitization Effects 0.000 title claims abstract description 9
- 235000020247 cow milk Nutrition 0.000 title abstract description 4
- 102000008192 Lactoglobulins Human genes 0.000 title description 11
- 102000000119 Beta-lactoglobulin Human genes 0.000 claims abstract 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims description 2
- 210000004080 milk Anatomy 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000043 antiallergic agent Substances 0.000 abstract description 2
- 239000002778 food additive Substances 0.000 abstract description 2
- 235000013376 functional food Nutrition 0.000 abstract description 2
- 238000003149 assay kit Methods 0.000 abstract 1
- 239000003547 immunosorbent Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000003266 anti-allergic effect Effects 0.000 description 10
- 208000009793 Milk Hypersensitivity Diseases 0.000 description 4
- 201000010859 Milk allergy Diseases 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 101001008231 Bos taurus Beta-lactoglobulin Proteins 0.000 description 3
- 208000004262 Food Hypersensitivity Diseases 0.000 description 3
- 206010016946 Food allergy Diseases 0.000 description 3
- 235000020932 food allergy Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 241000219000 Populus Species 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 206010012438 Dermatitis atopic Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 201000009961 allergic asthma Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 201000005356 cow milk allergy Diseases 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Pulmonology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Genetics & Genomics (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
本发明公开了一种可降低牛乳β乳球蛋白致敏的多肽，其特征在于它的序列：氨基端‑VAGTWYSL‑羧基端。本肽的抗致敏性效果经β乳球蛋白酶联免疫吸附测定试剂盒测定。结果表明，该肽对β乳球蛋白的致敏性有较好的抑制作用，可以进一步开发成β乳球蛋白的抗过敏药物或者功能食品添加剂。
The invention discloses a polypeptide that can reduce the sensitization of β-lactoglobulin in cow's milk, which is characterized in that its sequence: amino-terminal-VAGTWYSL-carboxy-terminal. The anti-allergenic effect of this peptide was determined by β-lactoglobulin-linked immunosorbent assay kit. The results show that the peptide has a good inhibitory effect on the sensitization of β-lactoglobulin, and can be further developed into an anti-allergic drug or functional food additive of β-lactoglobulin.
Description
The technical field is as follows:
the invention relates to a polypeptide capable of reducing protein sensitization, in particular to a polypeptide capable of reducing cow milk beta lactoglobulin sensitization.
Background art:
food allergy is a disease most common in infants and young children in the world today, affecting about 8% of infants and 4% of adults, while among children food allergy is most common Cow's Milk Allergy (CMA), which accounts for almost 2.5% of food allergy, and cow's milk allergy is closely linked to atopic eczema and allergic asthma. Research shows that 20% of cow milk allergy is caused by beta lactoglobulin and can cause other concomitant diseases of children, and research shows that some natural small molecules such as flavonoids can change covalent or non-covalent actions of lactoglobulin, so that the secondary structure or the tertiary structure of the beta lactoglobulin is changed, the allergic sites of the lactoglobulin are changed, and the anti-allergic effect is generated. Many small molecules even have certain toxicity or side effects, so the development of polypeptide antiallergic polypeptides which are nontoxic and harmless and can increase the nutrient absorption of human bodies has important practical significance.
The invention content is as follows:
in view of the above, in order to solve the above problems, we provide a polypeptide capable of reducing bovine milk beta lactoglobulin sensitization, the amino acid sequence of which is: the amino terminal-VAGTWYSL-carboxyl terminal can be tried to be developed into an antiallergic drug and a functional food additive of the bovine beta lactoglobulin.
Description of the drawings:
in order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described in detail with reference to the accompanying drawings, in which:
FIG. 1 shows the results of the measurement of the antiallergic effect of the newly designed peptides on bovine beta lactoglobulin at a concentration of 1X 10-5mol/L, the mol ratio of the newly designed peptide to the beta lactoglobulin is 1:1,2:1,3:1,4:1,5:1 respectively, the reaction time is 1.5h, and the pH is 7.0; the pH set experiment is set to be respectively 5.0, 6.0, 7.0, 8.0 and 9.0, the reaction time is 1.5h, and the molar ratio is 3: 1; the reaction time groups are respectively set to be 0.5h, 1.0h, 1.5h, 2.0h and 2.5h, the molar ratio is 3:1, and the reaction pH is 7.0.
The specific implementation mode is as follows:
the antiallergic effect is measured by adopting a bovine beta lactoglobulin (beta-LG) enzyme-linked immunosorbent assay kit and adopting a single-factor experiment, taking out the required lath from an aluminum foil bag after the room temperature is balanced for 60min, and sealing the rest laths by using a sealing bag and putting back the laths at 4 ℃. Standard substance holes, blank holes and poplar holes are arranged, and 50 microliters of standard substances with different concentrations are added in the standard substance holes respectively. 50 microliters of sample to be tested is added into the poplar well, and 50 microliters of dilution is added into the blank well. Blank wells, standard wells and sample wells were loaded with 50 microliters of this diluent. 100 microliters of detection antibody labeled with horseradish peroxidase (HRP) was added to each of the blank wells, the standard wells and the sample wells, the reaction wells were sealed with a sealing plate film, and incubated in a 37 ℃ water bath or incubator for 60 min. Discarding the liquid, patting dry on absorbent paper, filling 350 microliters of washing liquid into each hole, standing for 1min, throwing off the washing liquid, patting dry on the absorbent paper, and repeating the plate washing for 5 times. 50. mu.l each of the A, B substrate was added to the cells and incubated at 37 ℃ for 15min in the absence of light. Stop solution was added to each well in an amount of 50. mu.l, and the OD value of each well was measured at a wavelength of 450nm within 15 min. According to the ELISA result, the best antiallergic effect is that when the molar ratio is 4:1, the antiallergic effect of the newly designed peptide is the best, and reaches 38.71%; when the pH value is 9.0, the antiallergic effect of the newly designed peptide is optimal and reaches 27.94%; when the reaction time is 1.0h, the antiallergic effect is best, and 37.72% is achieved. Table 1 shows the anti-allergic activity of each experimental group. The result shows that the newly designed peptide has good effect of reducing beta lactoglobulin sensitization, and the sequence is as follows: amino-terminal-VAGTWYSL-carboxyl-terminal.
TABLE 1 antiallergic Activity
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and it is apparent that those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011434624.6A CN112694516A (en) | 2020-12-10 | 2020-12-10 | Polypeptide capable of reducing sensitization of beta lactoglobulin in cow milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011434624.6A CN112694516A (en) | 2020-12-10 | 2020-12-10 | Polypeptide capable of reducing sensitization of beta lactoglobulin in cow milk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112694516A true CN112694516A (en) | 2021-04-23 |
Family
ID=75507025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011434624.6A Pending CN112694516A (en) | 2020-12-10 | 2020-12-10 | Polypeptide capable of reducing sensitization of beta lactoglobulin in cow milk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112694516A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114717285A (en) * | 2022-04-12 | 2022-07-08 | 重庆大学 | A method for extracting soybean antioxidant peptides by enzymatic hydrolysis |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008108649A2 (en) * | 2007-03-06 | 2008-09-12 | Friesland Brands B.V. | Ace-inhibitory peptides from whey and methods for providing the same. |
| CN107427553A (en) * | 2015-03-18 | 2017-12-01 | N·V·努特里奇亚 | Method for inducing oral tolerance by administration of β-lactoglobulin-derived peptides in combination with probiotics |
| CN107853697A (en) * | 2011-12-07 | 2018-03-30 | N.V.努特里奇亚 | β‑lactoglobulin peptides for the treatment of bovine milk protein allergy |
| CN112218547A (en) * | 2018-04-30 | 2021-01-12 | N·V·努特里奇亚 | Formulations containing specific beta-lactoglobulin peptides |
| CN114630584A (en) * | 2019-11-07 | 2022-06-14 | 纽崔西亚公司 | Deep whey protein hydrolysate with tolerogenic peptides |
-
2020
- 2020-12-10 CN CN202011434624.6A patent/CN112694516A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008108649A2 (en) * | 2007-03-06 | 2008-09-12 | Friesland Brands B.V. | Ace-inhibitory peptides from whey and methods for providing the same. |
| CN107853697A (en) * | 2011-12-07 | 2018-03-30 | N.V.努特里奇亚 | β‑lactoglobulin peptides for the treatment of bovine milk protein allergy |
| CN107427553A (en) * | 2015-03-18 | 2017-12-01 | N·V·努特里奇亚 | Method for inducing oral tolerance by administration of β-lactoglobulin-derived peptides in combination with probiotics |
| CN112218547A (en) * | 2018-04-30 | 2021-01-12 | N·V·努特里奇亚 | Formulations containing specific beta-lactoglobulin peptides |
| CN114630584A (en) * | 2019-11-07 | 2022-06-14 | 纽崔西亚公司 | Deep whey protein hydrolysate with tolerogenic peptides |
Non-Patent Citations (2)
| Title |
|---|
| LANG CHEN等: "Experimental and computational studies on the mechanism of the β-lactoglobulin-derived peptide inhibiting the antigenicity of β-lactoglobulin", 《FOOD CHEMISTRY》 * |
| 齐晓彦等: "水解配方乳粉的抗过敏性研究进展及其市场开发应用", 《食品工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114717285A (en) * | 2022-04-12 | 2022-07-08 | 重庆大学 | A method for extracting soybean antioxidant peptides by enzymatic hydrolysis |
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Application publication date: 20210423 |