CN112656887B

CN112656887B - Traditional Chinese medicine composition with anti-inflammatory and antioxidant functions, preparation and preparation method thereof

Info

Publication number: CN112656887B
Application number: CN202110115194.XA
Authority: CN
Inventors: 肖丹; 尹超; 程远芳; 陶劲坤
Original assignee: Chengdu Grass And Wood Jinhua Science And Technology Co ltd
Current assignee: Chengdu Grass And Wood Jinhua Science And Technology Co ltd
Priority date: 2021-01-27
Filing date: 2021-01-27
Publication date: 2022-05-06
Anticipated expiration: 2041-01-27
Also published as: CN112656887A

Abstract

The invention discloses a traditional Chinese medicine composition with anti-inflammatory and antioxidant functions, belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to the technical field of anti-inflammatory and antioxidant traditional Chinese medicine compositions, aiming at solving the defects that the anti-inflammatory and antioxidant capabilities of cultured animals are deteriorated and the immunity is reduced because the existing antibiotics cannot be added into feed, and the traditional Chinese medicine composition comprises the following components in percentage by weight: 1-80% of Chinese yam, 1-80% of pomegranate peel, 1-20% of dried orange peel, 1-20% of valeriana jatamansi jones, 5-10% of hawthorn, 1-5% of perilla leaf, and 6 medicines of Chinese yam, pomegranate peel, dried orange peel, valeriana jatamansi jones, hawthorn and perilla leaf are used together, so that the effects of treating both principal and secondary aspects of disease, eliminating pathogenic factors, not forgetting and strengthening body resistance, combining sour harvest and pungent movement, and playing a role in resisting diarrhea, resisting inflammation and oxidation, promoting growth and improving immunity are achieved.

Description

Traditional Chinese medicine composition with anti-inflammatory and antioxidant functions, preparation and preparation method thereof

Technical Field

The invention discloses a traditional Chinese medicine composition with anti-inflammatory and antioxidant functions, a preparation and a preparation method thereof, belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to the technical field of anti-inflammatory and antioxidant traditional Chinese medicine compositions.

Background

The hormone is a medicine with wide application and remarkable treatment effect. Hormonal drugs can be classified into: glucocorticoids, adrenocorticoids, norepinephrine, progestins, estrogens, androgens, and the like.

Glucocorticoid (GC) is an extremely important regulatory molecule in the body, plays an important role in regulating development, growth, metabolism, immune function and the like of the body, is the most important regulatory hormone for stress response of the body, and is also the most widely and effectively anti-inflammatory and immunosuppressant in clinic. The clinically common glucocorticoid medicaments comprise prednisone, methylprednisolone, betamethasone, beclomethasone propionate, prednisolone, hydrocortisone, dexamethasone and the like. Has antiinflammatory, antitoxic, antiallergic, antishock, nonspecific immunity inhibiting and antipyretic effects, and can prevent and stop immune inflammatory reaction and pathological immune reaction, and is almost effective for allergic diseases of any type.

The antibiotic is added into the feed, so that the disease of the livestock and poultry can be prevented, the metabolism of the livestock and poultry can be improved, the growth of the livestock and poultry can be promoted, and the survival rate and the feed conversion rate can be improved. In addition, the anti-inflammatory and antioxidant capacity of the cultured animals can be improved, and the immunity is improved. The department of agriculture issues a notification about national animal source bacteria resistance inhibition action plan (2017 + 2020), and after a few days, the department of agriculture publishes a notification about animal antibacterial use reduction action test point development work in offices in the department of agriculture countryside, and organizes and formulates an animal antibacterial use reduction action test point working scheme (2018 + 2021). Before 2020, the resistance is forbidden at the feed end, and the resistance reduction and resistance reduction at the breeding end are inevitable!

Disclosure of Invention

The invention aims to: provides a traditional Chinese medicine composition with anti-inflammatory and anti-oxidation functions, a preparation and a preparation method thereof, and aims to overcome the defects that the anti-inflammatory and anti-oxidation capabilities of cultured animals are deteriorated and the immunity is reduced because the existing antibiotics cannot be added into feed.

The technical scheme adopted by the invention is as follows:

the traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 1-80% of Chinese yam, 1-80% of pomegranate peel, 1-20% of dried orange peel, 1-20% of valeriana jatamansi, 5-10% of hawthorn and 1-5% of perilla leaf.

In the technical scheme of the application:

the yam is sweet and mild in nature and taste, is not greasy and dry, and has the effects of tonifying spleen and qi, tonifying spleen qi and tonifying spleen yin; the yam can not only strengthen the spleen and replenish qi, but also alleviate diarrhea with slight astringency, and is good at treating the symptoms of spleen deficiency loose stool or diarrhea; contains amylase, polyphenol oxidase, etc., and is beneficial to the digestion and absorption function of spleen and stomach; the copper ions, connective tissues and calcium in the Chinese yam are greatly helpful for livestock and poultry, have the function of enhancing immunity, also contain rich DHEA, are derivatives of cyclopentane polyhydrophenanthrene, and have the functions of resisting aging, enhancing immunity, enhancing the capability of resisting diseases and accelerating the repair of damaged tissues;

the dried pericarp of pomegranate of Punicaceae is sour and astringent, warm in nature, and has effects of large intestine meridian, relieving diarrhea with astringents, stopping bleeding, expelling parasites, resisting bacteria, fungi and virus, improving immunity, and resisting oxidation.

The dried orange peel is also called as orange peel, has the effects of regulating qi, strengthening spleen, eliminating dampness, harmonizing stomach, stimulating appetite, strengthening spleen, promoting qi circulation, stopping diarrhea and improving bile secretion capacity, and the volatile oil in the dried orange peel has the effects of eliminating phlegm, relieving asthma and expanding bronchus, can effectively stimulate the smooth muscle of gastrointestinal tract and is beneficial to promoting the secretion of digestive juice, so that the effects of eliminating intestinal tract pneumatosis and enhancing appetite can be achieved, and the effects of aromatic stomach strengthening and bitter taste discharge of the dried orange peel can be exerted; the dried orange peel contains flavonoid compounds, has strong effects of inhibiting the automatic oxidation of animal oil, removing hydroxyl free radicals (OH) and the like, can inhibit the lipid peroxidation of animal brain, heart and liver tissues, can also enhance the relative activity of SOD enzyme, and also has the effects of inhibiting oxidation and protecting liver;

the valeriana jatamansi jones have the effects of regulating qi to alleviate pain, helping digestion to stop diarrhea, dispelling wind and removing dampness, and tranquilizing and allaying excitement, and are used for treating abdominal distension and pain, indigestion, diarrhea and dysentery, rheumatism and arthralgia and the like.

The hawthorn is sour, sweet and slightly warm in nature and taste, has the effects of promoting digestion, invigorating stomach, promoting blood circulation and promoting qi circulation, can enter spleen and stomach to eliminate food retention and stagnation, disperse blood, can also treat water dysentery, not only helps pomegranate rind to stop diarrhea to treat the primary syndrome, but also helps to eliminate food retention and strengthen spleen and stomach to promote growth; the lipase is contained, so that the fat digestion can be promoted, the secretion of gastric digestive enzyme can be increased, and the digestion can be promoted; the hawthorn water extract has the function of removing O₂Free radical, lipid peroxidation inhibition, antioxidation, immunity enhancement, antibacterial and analgesic effects;

folium Perillae, pungent and fragrant, and has effects of activating qi-flowing, activating spleen, stimulating appetite, increasing aroma, inhibiting odor, treating affection of exogenous wind-cold, and stagnation of qi due to dampness of spleen and stomach, contains volatile oil, flavone, phenolic acid, anthocyanin, polysaccharide, triterpene, and steroid, and has multiple biological activities such as antiinflammatory, antioxidant, antibacterial, antidepressant, antitumor, and tranquilizing;

the yam and the pomegranate rind are used for relieving diarrhea with astringents, mutual reinforcement and synergy; the Chinese yam is used as monarch drug for strengthening body resistance and tonifying deficiency, and the pomegranate bark can eliminate pathogenic factors and supplement each other; the dried orange peel and the valeriana jatamansi jones are used for assisting in regulating qi, strengthening spleen, eliminating dampness, harmonizing stomach, tranquilizing and promoting growth, have anti-inflammatory and antioxidant effects and are ministerial drugs; the hawthorn and the perilla leaf can help digestion and stop diarrhea, or eliminate dampness and regulate the middle warmer, or deodorize and enhance flavor, and can be used as assistant and guide products together, thereby conforming to the compatibility principle of 'assistant, minister, assistant and guide'. The traditional Chinese medicine composition has the effects of treating both manifestation and root cause of disease, eliminating pathogenic factors, strengthening body resistance, combining sour harvest and pungent flavor, and playing the effects of resisting diarrhea, resisting inflammation and oxidation, promoting growth and improving immunity.

The traditional Chinese medicine composition disclosed by the application starts from controlling diarrhea, protects the gastrointestinal mucosa damaged by the diarrhea and promotes repair, removes intercellular oxygen radicals, controls the extravasation of interstitial fluid, and rebalances the intracellular and extracellular environments.

In the diarrhea state, the condition that only the environment in the digestive tract is balanced is not enough to eliminate the symptoms, the process that the body automatically restores to be in the normal state is a long process, and the disease condition is repeated possibly. From the long-term effect, the organism in the state of vigorous immunity is not easily influenced by the external environment, from the cultivation aspect, the cultivation environment, such as the change of temperature, humidity, infectious diseases and the like, is not easily influenced by the growth of cultivated animals, the valeriana jatamansi jones and the perilla leaves have various efficacies of inflammation resistance, oxidation resistance, virus resistance, bacteria resistance and the like, and are combined with the dried orange peel and the hawthorn which have the function of promoting digestion and absorption, so that the function of promoting growth is finally achieved.

Preferably, the traditional Chinese medicine composition comprises the following components in percentage by weight: 1-79% of Chinese yam, 1-79% of pomegranate peel, 5-10% of dried orange peel, 5-10% of valeriana jatamansi, 5-7% of hawthorn and 1-5% of perilla leaf.

Preferably, the traditional Chinese medicine composition comprises the following components in percentage by weight: 10-70% of Chinese yam, 10-70% of pomegranate bark, 5-12% of dried orange peel, 5-12% of valeriana jatamansi, 5-8% of hawthorn and 1-5% of perilla leaf.

Preferably, the traditional Chinese medicine composition comprises the following components in percentage by weight: 20-60% of Chinese yam, 20-60% of pomegranate peel, 5-15% of dried orange peel, 5-15% of valeriana jatamansi, 5-9% of hawthorn and 1-5% of perilla leaf.

Preferably, the traditional Chinese medicine composition comprises the following components in percentage by weight: 30-45% of Chinese yam, 30-45% of pomegranate peel, 5-17% of dried orange peel, 5-17% of valeriana jatamansi, 5-10% of hawthorn and 1-5% of perilla leaf.

Preferably, the traditional Chinese medicine composition comprises the following components in percentage by weight: 35% of Chinese yam, 35% of pomegranate peel, 10% of dried orange peel, 9.5% of valeriana jatamansi, 7.5% of hawthorn and 3% of perilla leaf.

A Chinese medicinal composition with antiinflammatory and antioxidant effects is provided.

The preparation can be any one of tablets, granules, powder and pills.

A preparation method of the preparation comprises the steps of crushing the Chinese yam, the pomegranate rind, the dried orange peel, the valeriana jatamansi jones, the hawthorn and the perilla leaf according to the weight percentage, and preparing the preparation according to a conventional method.

In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

1. in the invention, the Chinese yam and the pomegranate rind are used for relieving diarrhea with astringents, and mutual reinforcement and synergy are realized; the Chinese yam is used as monarch drug for strengthening body resistance and tonifying deficiency, and the pomegranate bark can eliminate pathogenic factors and supplement each other; the dried orange peel and the valeriana jatamansi jones are used for assisting in regulating qi, strengthening spleen, eliminating dampness, harmonizing stomach, tranquilizing and promoting growth, have anti-inflammatory and antioxidant effects and are ministerial drugs; the hawthorn and the perilla leaf can help digestion and stop diarrhea, or eliminate dampness and regulate the middle warmer, or deodorize and enhance flavor, and can be used as assistant and guide products to meet the compatibility principle of 'assistant, minister, assistant and guide';

2. according to the invention, 6 medicines of Chinese yam, pomegranate rind, dried orange peel, valeriana jatamansi jones, hawthorn and perilla leaf are used together, so that both the principal and secondary aspects of the disease are considered, the pathogenic factors are eliminated, the body resistance is not forgotten, the sour harvest and the pungent flavor are combined, and the diarrhea resistance, the anti-inflammatory and the anti-oxidation effects are achieved together, the growth is promoted, and the immunity is improved;

3. according to the invention, the traditional Chinese medicine composition can effectively reduce the inflammation level of Kunming mice, has a certain antioxidation effect and has a certain dose-effect relationship.

Drawings

FIG. 1 is the effect of TK of the invention on body weight of Kunming mice;

FIG. 2 is a graph of the effect of TK of the invention on Kunming mouse spleen index;

FIG. 3 is the effect of TK of the invention on Kunming mouse thymus index;

FIG. 4 is the effect of TK of the invention on IL-1 β in serum of Kunming mice;

FIG. 5 is the effect of TK of the invention on IL-6 in serum of Kunming mice;

FIG. 6 is the effect of TK of the invention on IL-6 in the spleen of Kunming mice;

FIG. 7 is the effect of TK of the invention on TNF- α in serum of Kunming mice;

FIG. 8 is a graph of the effect of TK of the invention on TNF- α in the spleen of Kunming mice;

FIG. 9 is the effect of TK of the invention on SOD in serum of Kunming mice;

FIG. 10 is the effect of TK of the invention on MDA in Kunming mouse serum;

FIG. 11 is the effect of TK of the invention on MDA in the spleen of Kunming mice.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Example 1

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 79% of Chinese yam, 1% of pomegranate peel, 6.5% of dried orange peel, 6% of valeriana jatamansi, 5.5% of hawthorn and 2% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder. The yam and the pomegranate rind jointly form the main components of the scheme, the two components are matched to realize the main effects, and the proportion mainly considers the balance relationship between the effect and the material cost.

Example 2

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 1% of Chinese yam, 79% of pomegranate peel, 6.5% of dried orange peel, 6% of valeriana jatamansi, 5.5% of hawthorn and 2% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder. The yam and the pomegranate rind jointly form the main components of the scheme, the two components are matched to realize the main effects, and the proportion mainly considers the balance relationship between the effect and the material cost.

Example 3

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 70% of Chinese yam, 10% of pomegranate peel, 6.5% of dried orange peel, 6.5% of valeriana jatamansi, 5% of hawthorn and 2% of perilla leaf. The preparation method comprises pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 4

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 10% of Chinese yam, 70% of pomegranate peel, 6.5% of dried orange peel, 6.5% of valeriana jatamansi, 5% of hawthorn and 2% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 5

The traditional Chinese medicine composition with the functions of inflammation resistance and oxidation resistance comprises the following components in percentage by weight: 60% of Chinese yam, 20% of pomegranate peel, 6% of dried orange peel, 6% of valeriana jatamansi, 5.5% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 6

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 20% of Chinese yam, 60% of pomegranate peel, 6% of dried orange peel, 6% of valeriana jatamansi, 5.5% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 7

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 45% of Chinese yam, 30% of pomegranate peel, 8% of dried orange peel, 7.5% of valeriana jatamansi, 7% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 8

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 30% of Chinese yam, 45% of pomegranate peel, 8% of dried orange peel, 7.5% of valeriana jatamansi, 7% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 9

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 38% of Chinese yam, 38% of pomegranate peel, 8% of dried orange peel, 8% of valeriana jatamansi, 6% of hawthorn and 2% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 10

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 36% of Chinese yam, 36% of pomegranate rind, 9.5% of dried orange peel, 8.5% of valeriana jatamansi jones, 7.5% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 11

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 35% of Chinese yam, 35% of pomegranate peel, 10% of dried orange peel, 9.5% of valeriana jatamansi, 8% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 12

The traditional Chinese medicine composition with the functions of resisting inflammation and resisting oxidation comprises the following components in percentage by weight: 35% of Chinese yam, 34% of pomegranate peel, 10% of dried orange peel, 9.5% of valeriana jatamansi, 9% of hawthorn and 2.5% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

Example 13

The traditional Chinese medicine composition with the functions of inflammation resistance and oxidation resistance comprises the following components in percentage by weight: 34% of Chinese yam, 34% of pomegranate peel, 10% of dried orange peel, 10% of valeriana jatamansi, 9% of hawthorn and 3% of perilla leaf. The preparation method comprises the following steps: pulverizing the above Chinese medicinal materials in percentage by weight, mixing, and making into powder.

The tablets, granules or pills can also be prepared by adopting medically acceptable auxiliary materials according to a conventional method in the examples 1 to 13 of the application.

Test examples

The anti-inflammatory and antioxidant effects of the traditional Chinese medicine composition powder prepared in example 5, hereinafter referred to as TK drug, on Kunming mice were studied:

materials and reagents

Animals including SPF-level Kunming mouse, female mouse, 4-6 weeks old, body weight 16 + -2 g, 7 groups including 6 mice and 42 mice;

LPS, TK, 0.3% CMC, and dexamethasone;

the kit comprises: ELISA detection kits for IL-6, IL-1 beta, TNF-alpha and the like (American Saimei Fei); MDA, SOD biochemical detection kit (built by Nanjing); peroxidase antibody (U.S. CST); quantitative PCR kit and cytokine primers (Shanghai's worker) such as IL-6, IL-1 beta, TNF-alpha and the like.

Second, method

Mice were randomly divided by weight:

normal control group (NC): irrigating equal amount of CMC for 1 time/d;

inflammation model group (LPS): irrigating equal amount of CMC for 1 time/d;

dexamethasone control group (DXM): irrigating equal amount of CMC for 1 time/d;

TK low dose group (TK-L): irrigating 0.2g/kg TK for 1 time/d;

TK middle dose group (TK-M): irrigating 0.8g/kg TK for 1 time/d;

TK high dose group (TK-H): drench 3.2g/kg TK, 1 time/d.

The equivalent CMC is infused, namely the CMC is correspondingly given to other control groups with the same volume according to the dose of the injected TK drug, the weight average value of the experimental mouse does not exceed 25g, and the infusion dose setting standard is that the administration volume of each 10g weight mouse is 0.2ml, so that the administration volume of the experiment is 0.2ml/10 g/25 g-0.5 ml/mouse.

Dosing and modeling:

each group of experimental animals was administered 1 time per day at a dose and manner of 6d continuously. All animals were fasted without water deprivation after dosing on day 6. Except for the normal control group, LPS was intraperitoneally injected at a dose of 30mg/kg once after the last administration of 30min on day 7 (the positive control group was intraperitoneally injected with dexamethasone injection (10mg/kg) on day 7) in each of the other groups for establishing a mouse acute inflammation model. Immediately performing orbital bleeding after 6h, standing at 4 ℃ for at least 30min, centrifuging at 3000r/min for 10min, collecting supernatant, subpackaging, and storing in a refrigerator at-80 ℃ for later use. The collected animal is killed by dislocation of cervical vertebra, spleen, thymus, liver and pancreas of the mouse are dissected and taken, weighed and respectively preserved in a refrigerator with the temperature of 80 ℃ below zero and 4% paraformaldehyde solution for fixation for later use.

Research shows that the acute inflammation model in the body of the mouse can be successfully established by injecting 10-15mg/kg LPS into the abdominal cavity, but the previous experimental results of the inventor show that the inflammation model is not successfully established by injecting the LPS into the abdominal cavity, so that through continuous research and research of a subject group, the LPS dose is finally fixed at 30mg/kg, and meanwhile, the modeling success rate and the survival rate of the mouse are considered.

Third, detecting the index

Weighing the body weight 1 time every 3 days;

organ index: spleen, liver, pancreas and thymus, for example, spleen index determination, the body mass of a dissected mouse is weighed, the spleen of the mouse is taken, and the spleen index is determined. Spleen index formula spleen index equals spleen mass (mg)/body mass (g), and other indices were calculated in the same manner. (spleen and thymus are important immune organs, spleen index and thymus index are significant in prompting the strong and weak state of immunity; if the spleen index of an experimental group is higher than that of a control group, the immunity of the organism is prompted to be enhanced; on the contrary, some cytotoxic drugs such as cyclophosphamide have very strong immunosuppressive action and can comprehensively reduce the immune function of the organism, so that the index is reduced, and the index change of an immunomodulator is not obvious.)

Detecting the expression levels of inflammatory factors (kit) IL-6, IL-1 beta, TNF-alpha and the like in serum by an ELISA method;

detecting the expression of inflammatory factors IL-6, IL-1 beta and TNF-alpha mRNA in tissues by a PCR method;

detecting related indexes of antioxidation by a biochemical method (kit): MDA, SOD;

examples of pathological tissue structure changes of organs (spleen, liver, pancreas and thymus are selected according to actual anatomical conditions for HE staining): observing the change of the mouse liver tissue structure (HE staining), dissecting the mouse liver tissue, fixing by 4% paraformaldehyde solution, taking materials conventionally, dehydrating, dipping in wax, embedding, flaking (4 μm thick), dewaxing, hydrating, hematoxylin-eosin staining, and observing the structural change of the mouse liver tissue after scanning by a panoramic scanner;

immunohistochemical detection of peroxidase (determined according to 5 and 6 experimental results) is carried out by subjecting prepared paraffin sections of liver to deparaffinization and water bloom, thermoantigen repair and endogenous peroxidase inactivation by 3% H2O2, blocking nonspecific sites with goat serum, adding primary antibody, incubating overnight in a refrigerator at 4 ℃, incubating secondary antibody, staining with DAB and hematoxylin, dehydrating, permeabilizing, sealing with neutral gum, microscopic examination and Image analysis by Image-Proplus.6.0 software.

Fourth, data statistics

Statistical differences of the measurement indexes of each group are analyzed by adopting one-way analysis of variance (ANOVA), all statistical analysis is completed under SPSS Statistics 18.0 software, and p is less than 0.05 and less than 0.01, which indicates that the differences have statistical significance.

Fifth, test results

1. Body weight

The Body weight of each group of mice is recorded in Table 1, FIG. 1 (Body weight (g) in FIG. 1 is Body weight (g)). As can be seen from the data in the graph, the weight values of all groups have no statistical difference (p is more than 0.05), and the TK medicament has low toxicity and no obvious influence on the weight of the mice.

The body weight of each group of mice is recorded in table 1, fig. 1. As can be seen from the data in the graph, the weight values of all groups have no statistical difference (p is more than 0.05), and the TK medicament has low toxicity and no obvious influence on the weight of the mice.

TABLE 1 Effect of TK on Kunming mouse weight (` x. + -. s, g)

Note: compared with the normal control group, the composition has the advantages that,^#p<0.05，^##p<0.01, p compared to model control group<0.05，**p<0.01

2. Index of visceral organs

2.1 spleen index

The Spleen index results are shown in Table 2, and FIG. 2(Spleen index (mg/g) is the Spleen index (mg/g)), in FIG. 2: compared with the normal control group, the composition has the advantages that,^##p<0.01, p compared to model control group<0.05，**p<0.01. Spleen index was significantly increased in the model group compared to the normal group (p)<0.01), the spleen swelling degree of the model group is far higher than that of the normal group. Compared with the model group, the spleen swelling degree of the dexamethasone group, the TK-M group and the TK-H group is obviously reduced compared with the model group (p)<0.05 or p<0.01)。

TABLE 2 influence of TK on Kunming mouse spleen index (` x. + -. s, g)

2.2. Index of thymus

The Thymus index results are shown in Table 3, FIG. 3(Thymus index (mg/g)) in FIG. 3: compared with the normal control group, the composition has the advantages that,^##p<0.01, p compared to model control group<0.05，**p<0.01. Compared with the model group, the thymus swelling degree of the dexamethasone group, the TK-M group and the TK-H group is obviously reduced compared with the model group (p)<0.05 or p<0.01)。

TABLE 3 influence of TK on Kunming mouse thymus index (` x. + -. s, g)

3. Inflammatory factors

3.1IL-1β

The results of ELISA detection of IL-1. beta. in mouse serum are shown in Table 4 and FIG. 4, and in FIG. 4, the following are noted: compared with the normal control group, the composition has the advantages that,^##p<0.01, p compared to model control group<0.05，**p<0.01, the IL-1 beta level in the model group is far higher than that in the normal group (p < 0.01), other administration groups can effectively reduce the IL-1 beta level (p < 0.01), and each TK group shows good dose dependence.

TABLE 4 ELISA detection of IL-1. beta. levels in mouse serum (` x. + -. s, pg/ml)

3.2IL-6

The results of ELISA detection of IL-6 in mouse serum are shown in Table 5 and FIG. 5,in fig. 5, note: compared with the normal control group, the composition has the advantages that,^##p<0.01, p compared to model control group<0.05，**p<0.01, the IL-6 level in the model group is far higher than that in the normal group (p is less than 0.01), and the TK-H group can effectively reduce the IL-6 level (p is less than 0.01).

TABLE 5 ELISA for serum IL-6 levels (` x. + -. s, pg/ml) in mice

Note: compared with the normal control group, the composition has the advantages that,^#p<0.05，^##p<0.01, p compared to model control group<0.05，**p<The results of 0.01ELISA assay for mouse spleen IL-6 are shown in Table 6 and FIG. 6, in FIG. 6, note: compared with the normal control group, the composition has the advantages that,^##p<0.01, p compared to model control group<0.05，**p<0.01, the IL-6 level in the model group is far higher than that in the normal group (p < 0.01), other administration groups can effectively reduce the IL-6 level (p < 0.01), and each TK group shows good dose dependence.

TABLE 6 detection of mouse spleen IL-6 levels by ELISA (` x. + -. s, pg/ml)

3.3TNF-α

The results of the ELISA assay for mouse serum TNF- α are shown in Table 7 and FIG. 7, in FIG. 7, note: compared with the normal control group, the composition has the advantages that,^##p<0.01, p compared to model control group<0.05，**p<0.01, TNF- α levels in the model group were much higher than in the normal group (p < 0.01). Compared with the model group, the dexamethasone group, the TK-M group and the TK-H group can effectively reduce the TNF-alpha level (p is less than 0.01), and each TK group has good dose dependence.

TABLE 7 ELISA detection of mouse serum TNF-. alpha.levels (` x. + -. s, pg/ml)

The results of ELISA assay of mouse spleen TNF-. alpha.are shown in Table 8 and FIG. 8, in FIG. 8, compared with the normal control group,^#p<0.05，^##p<0.01; p compared to model control group<0.05，**p<0.01, TNF- α levels in the model group were much higher than in the normal group (p < 0.01). Compared with the model group, the dexamethasone group and the TK-H group can effectively reduce the level of TNF-alpha (p is less than 0.01).

TABLE 8 ELISA detection of mouse spleen TNF-. alpha.levels (` x. + -. s, pg/ml)

4. Index of oxidation

4.1SOD

Superoxide dismutase (SOD) has the function of catalyzing superoxide anion free radicals to form hydrogen peroxide, and further decomposing the hydrogen peroxide into water and oxygen, so that the organism is protected from being damaged by oxidative stress. Therefore, the high and low levels of SOD activity directly reflect the body's ability to scavenge oxygen radicals.

The results of the microplate assay for detecting SOD in mouse serum are shown in Table 9 and FIG. 9, in FIG. 9, compared with the normal control group,^#p<0.05，^##p<0.01; p compared to model control group<0.05，**p<0.01, the SOD level in the model group is far lower than that in the normal group (p < 0.05). Compared with the model group, the TK-M and the TK-H groups can effectively increase SOD levels (p is less than 0.05), and each group of the TKExhibit good dose-dependence.

TABLE 9 microplate method for detecting mouse serum SOD activity level (` x. + -. s, U/ml)

Note: compared with the normal control group, the compound preparation,^#p<0.05，^##p<0.01, p compared to model control group<0.05，**p<0.01

4.2MDA

MDA (malondialdehyde) is one of lipid peroxide products, oxidation products such as lipofuscin and the like are further generated in a body through a series of reactions such as protein crosslinking and the like and are accumulated in body cells, the normal structure and function of the body cell membrane are damaged, the burden of the body tissues is increased, finally, the body tissue cells cannot maintain the normal metabolism of the cells and are aged and dead, and the content of the oxidation products can indirectly reflect the degree of lipid peroxidation in the body and the severity of the body cells under the attack of free radicals.

The results of MDA detection in mouse serum by the TBA method are shown in table 10 and fig. 10, and in fig. 10, compared with the normal control group,^#p<0.05，^##p<0.01; p compared to model control group<0.05，**p<0.01, the MDA level in the model group is much higher than that in the normal group (p < 0.05). Compared with the model group, dexamethasone, TK-M and TK-H can effectively reduce MDA level (p is less than 0.01), and each group of TK has good dose dependence.

TABLE 10 detection of mouse serum MDA Activity level by TBA method (` x. + -. s, U/ml)

The results of MDA detection in mouse spleen by TBA method are shown in Table 11 and FIG. 11, in FIG. 11, compared with the normal control group,^#p<0.05，^##p<0.01; p compared to model control group<0.05，**p<0.01, MDA level in the model group is much higher than that in the normal group (p < 0.01). Each treatment group was effective in reducing MDA levels (p < 0.05) compared to the model group.

TABLE 11 TBA assay for spleen MDA viability levels (` x. + -. s, U/ml) in mice

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. A traditional Chinese medicine composition with anti-inflammatory and antioxidant functions is characterized in that: the traditional Chinese medicine composition comprises the following components in percentage by weight: 60% of Chinese yam, 20% of pomegranate peel, 6% of dried orange peel, 6% of valeriana jatamansi, 5.5% of hawthorn and 2.5% of perilla leaf.

2. A preparation of the anti-inflammatory and antioxidant Chinese medicinal composition of claim 1.

3. The formulation of claim 2, wherein: the preparation can be any one of tablets, granules, powder and pills.

4. A method of preparing the formulation of claim 2, wherein: pulverizing rhizoma Dioscoreae, pericarpium Granati, pericarpium Citri Tangerinae, Valeriana jatamansi jones, fructus crataegi and folium Perillae by the above weight percentage, and making into preparation by conventional method.