CN112649610A - Detection kit and detection method for methamphetamine drugs in hair - Google Patents
Detection kit and detection method for methamphetamine drugs in hair Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 63
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- 239000003814 drug Substances 0.000 title claims abstract description 48
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 21
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- 241000283707 Capra Species 0.000 claims abstract description 15
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 15
- 238000003118 sandwich ELISA Methods 0.000 claims abstract description 4
- 239000004081 narcotic agent Substances 0.000 claims abstract 3
- 239000000243 solution Substances 0.000 claims description 77
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000002965 ELISA Methods 0.000 claims description 15
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 230000003472 neutralizing effect Effects 0.000 claims description 11
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 230000009089 cytolysis Effects 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 239000007974 sodium acetate buffer Substances 0.000 claims description 5
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims description 5
- 239000012086 standard solution Substances 0.000 claims description 5
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- 238000006243 chemical reaction Methods 0.000 claims description 3
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- KWTSXDURSIMDCE-QMMMGPOBSA-N (S)-amphetamine Chemical compound C[C@H](N)CC1=CC=CC=C1 KWTSXDURSIMDCE-QMMMGPOBSA-N 0.000 description 3
- 229940025084 amphetamine Drugs 0.000 description 3
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses a detection kit for methamphetamine drugs in hair, which comprises an extracting solution, an enzyme label plate coated with a mouse anti-methamphetamine monoclonal antibody, a detection antibody and an enzyme-labeled secondary antibody, wherein the detection antibody is a rabbit anti-methamphetamine polyclonal antibody, and the enzyme-labeled secondary antibody is goat anti-rabbit IgG-HRP. When the method for detecting the content of the methamphetamine narcotics in the hair by using the kit is used, the hair is cut into small sections and then placed in a container, and the narcotics are extracted by using the extracting solution to obtain the extracting solution to be detected; and detecting the content of the drugs in the extracting solution to be detected by using a double-antibody sandwich ELISA method. The method can be used for quantitatively detecting the methamphetamine drugs in the hair, can meet the requirement that the international detection threshold is 0.2ng/mg, and has good specificity and high sensitivity; simple operation, rapid extraction, and no need of special equipment.
Description
Technical Field
The invention belongs to the technical field of contraband monitoring and analysis, and particularly relates to a detection kit and a detection method for methamphetamine drugs in hair.
Background
The conventional test materials for testing whether drug addicts take drugs or not are body fluid test materials such as blood and urine. However, since many drug addicts carry infectious disease viruses, blood collection of the blood sample has certain danger to operators, and sometimes the blood collection of the person to be collected is not matched. Urine is examined the material and is easily polluted, and the on-the-spot sampling of urine is taken care of and is related to privacy, does not take care of and involves urine dilution or adulteration. Therefore, the method for extracting the test material is simple and rapid, the detection result is accurate, and the traceability is strong, which is very important. The national ban of drug addiction is revised from 'drug addiction identification method' in 2016, and hair samples are identified as one of the main bases of drug addiction. Compared with body fluid, the hair has the advantages of simple operation, stable storage, easy storage, difficult counterfeit, super long available detection time limit and the like, and can provide time and residual quantity for drug addicts to take amphetamine drugs.
At present, the detection technology of drugs mainly includes an ELISA (enzyme linked immunosorbent assay) method, a colloidal gold immunoassay method and the like. The colloidal gold detection method has the characteristics of simple operation, time saving, easy result interpretation and the like, and is widely used for detecting the amphetamine drugs in blood or urine, but cannot meet the requirement of the international threshold for hair drug detection due to low sensitivity, and cannot be applied to the detection of the methamphetamine in hair. And the sensitivity of the ELISA method can meet the detection requirement of the methamphetamine in the hair. The content of methamphetamine in the hair is low, and whether the methamphetamine drugs can be simply and quickly extracted from the hair detection material is avoided, so that the development cost of the detection kit and the detection time are related, and the accuracy of the detection result is also influenced; the international detection threshold value of the poison and the metabolite thereof in the hair is 0.2ng/mg, a technology with higher detection sensitivity is required to be used for hair detection, and the conventional detection method has insufficient specificity and is easy to cause false positive. Therefore, the research of the detection method which is simple and convenient to operate, rapid in extraction, independent of expensive instruments and high in detection specificity and sensitivity has important positive significance.
Disclosure of Invention
The invention aims to provide a detection kit and a detection method for detecting methamphetamine type drugs in hair, which are simple and convenient to operate, rapid to extract, independent of expensive instruments and high in detection specificity and sensitivity.
In order to realize the purpose of the invention, the invention provides a detection kit for methamphetamine drugs in hair, which comprises an extracting solution, an enzyme label plate coated with a mouse anti-methamphetamine monoclonal antibody, a detection antibody and an enzyme labeled secondary antibody, wherein the detection antibody is a rabbit anti-methamphetamine polyclonal antibody, and the enzyme labeled secondary antibody is goat anti-rabbit IgG-HRP (HRP-labeled goat anti-rabbit).
Preferably, the extracting solution comprises a lysis solution, and the lysis solution consists of a NaOH solution with the concentration of 1mol/L and a SDS solution with the mass concentration of 10%.
Preferably, the extracting solution further comprises a neutralizing solution, and the neutralizing solution is an acetic acid-sodium acetate buffer solution with the pH value of 9.6.
Preferably, the concentration of the mouse anti-methamphetamine monoclonal antibody in the elisa plate coated with the mouse anti-methamphetamine monoclonal antibody is 20 mug/mL.
Preferably, the detection antibody is a rabbit anti-methamphetamine polyclonal antibody diluted by 1:1000, and the enzyme-labeled secondary antibody is goat anti-rabbit IgG-HRP diluted by 1: 5000.
The invention also provides a detection method of the methamphetamine drugs in the hair, which uses the detection kit for the methamphetamine drugs in the hair to detect, and the detection method comprises the following steps:
s1, cutting the hair into small sections, placing the small sections in a container, and extracting the drugs by using the extracting solution to obtain the extracting solution to be detected;
s2, detecting the drug content in the extract to be detected by using a double-antibody sandwich ELISA method, which comprises the following steps:
s21, adding an extracting solution to be detected and a standard substance solution into an enzyme label plate coated with a mouse anti-methamphetamine monoclonal antibody according to 100 mu L/hole respectively, incubating at the constant temperature of 37 ℃ for 1.5h, and washing with a PBST solution for 3 times, wherein each time is 3 min; the standard solution is used for drawing a standard curve, and the concentrations of the standard solution are respectively as follows: 700ng/mL, 350ng/mL, 175ng/mL, 87.5ng/mL, 43.75ng/mL, 21.88ng/mL, 10.94 ng/mL;
s22, adding rabbit anti-methamphetamine polyclonal antibody diluted by 1:1000 into the holes of the ELISA plate treated in the step S21, adding 100 mu L of rabbit anti-methamphetamine polyclonal antibody into each hole, incubating at the constant temperature of 37 ℃ for 1h, and washing with PBST solution for 3 times, 3min each time;
s23, adding goat anti-rabbit IgG-HRP diluted by 1:5000 into the wells of the ELISA plate treated in the step S22, adding 100 mu L of the goat anti-rabbit IgG-HRP into each well, incubating at the constant temperature of 37 ℃ for 0.5h, and washing with PBST solution for 4 times, 3min each time;
s24, adding 100 mu L of substrate developing solution into each hole of the ELISA plate treated in the step 23, incubating for 15min-20min at constant temperature, adding 50 mu L of Elisa stop solution to stop the reaction, and measuring the light absorption value at 450nm within 30 min.
Preferably, in step S1, the method for obtaining the extract to be tested includes: taking 50mg of hair to be detected, scrubbing the hair for 1-3 times by using 70% ethanol, cutting the hair into the size of 0.5-1 cm by using scissors, transferring the hair into a centrifuge tube, adding 500 mu L of lysate, incubating the hair for 10min at 75 ℃, transferring 300 mu L of cracked product into a new centrifuge tube, adding 200 mu L of neutralizing solution, standing the hair for 1min at room temperature, centrifuging the hair for 5min at 12000rpm, and absorbing supernatant to obtain the extracting solution to be detected; the lysis solution is formed by mixing NaOH solution with the concentration of 1mol/L and SDS solution with the mass concentration of 10%; the neutralizing solution is acetic acid-sodium acetate buffer solution with the pH value of 9.6.
Preferably, in step S21, the process for preparing the elisa plate coated with the murine anti-methamphetamine monoclonal antibody comprises the following steps:
s211, dissolving a mouse anti-methamphetamine monoclonal antibody by using a carbonate coating buffer solution to enable the final concentration of the mouse anti-methamphetamine monoclonal antibody to be 20 mu g/mL, adding the mouse anti-methamphetamine monoclonal antibody into an enzyme label plate according to 100 mu L/hole, and standing overnight at 4 ℃;
s212, discarding the coating solution every other day, and washing with PBST solution for 3 times, each time for 3 min.
Preferably, in step S211, the concentration of the carbonate coating buffer is 50mmol/L, and the pH value is 9.6.
Preferably, in step S24, the substrate color developing solution is a two-component TMB color developing solution.
Compared with the prior art, the invention has the following beneficial effects:
1. the detection kit for the methamphetamine drugs in the hair can be used for quantitatively and qualitatively detecting the methamphetamine drugs in the hair, can meet the requirement that the international detection threshold is 0.2ng/mg, and has good specificity and high sensitivity;
2. the method for extracting the methamphetamine-type drugs from the hair does not need expensive instruments, can be directly used for subsequent detection after extraction, and has the advantages of simple and convenient operation and rapid extraction, and particularly, the time for cracking the hair is only 10 min;
3. the invention uses a double-antibody sandwich method to coat the mouse anti-methamphetamine monoclonal antibody, the detection antibody is a rabbit anti-methamphetamine polyclonal antibody, the generation of false positive can be reduced, the specificity of the detection result is improved, and the invention has the advantage of high detection specificity.
Drawings
FIG. 1: example 1 standard graph of testing.
Detailed Description
The invention aims to provide a detection kit and a detection method for detecting methamphetamine type drugs in hair, which are simple and convenient to operate, rapid to extract, independent of expensive instruments and high in detection specificity and sensitivity. For a more clear presentation, the invention is described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. The raw materials and equipment used in the present invention are commercially available unless otherwise specified.
Example 1:
the embodiment discloses a detection reagent for methamphetamine drugs in hair, and the kit is used for detecting the content of the methamphetamine drugs in the hair by an ELISA method. The reagent comprises an extracting solution, an enzyme label plate coated with a mouse anti-methamphetamine monoclonal antibody, a detection antibody and an enzyme-labeled secondary antibody, wherein the detection antibody is a rabbit anti-methamphetamine polyclonal antibody, and the enzyme-labeled secondary antibody is goat anti-rabbit IgG-HRP. The extracting solution comprises a lysis solution, wherein the lysis solution consists of a NaOH solution with the concentration of 1mol/L and an SDS solution (Sodium dodecyl sulfate, Chinese name is Sodium dodecyl sulfate, and is also called Sodium dodecyl sulfate) with the mass concentration of 10%. The extracting solution also comprises a neutralizing solution, and the neutralizing solution is an acetic acid-sodium acetate buffer solution with the pH value of 9.6. In the ELISA plate coated with the mouse anti-methamphetamine monoclonal antibody, the concentration of the mouse anti-methamphetamine monoclonal antibody is 20 mug/mL. The detection antibody is a rabbit anti-methamphetamine polyclonal antibody diluted by 1:1000, and the enzyme-labeled secondary antibody is goat anti-rabbit IgG-HRP diluted by 1: 5000.
The embodiment also provides a method for detecting the content of methamphetamine drugs in hair by using the kit, and the detection method comprises the following steps:
1. the method for quickly extracting the methamphetamine drugs in the hair comprises the following steps:
taking 50mg of hair, scrubbing the hair for 1-3 times by using 70% ethanol, cutting the hair into the size of 0.5cm-1cm by using scissors, transferring the hair into a 1.5mL centrifuge tube, adding 500 mu L of lysate (final concentration: 1M NaOH, 0.5% SDS), incubating for 10min at 75 ℃, transferring 300 mu L of the cracked product into a new 1.5mL centrifuge tube, adding 200 mu L of neutralizing solution, standing for 1min at room temperature (preferably 26 ℃), centrifuging for 5min at 1200rpm, sucking supernatant into a clean new 1.5mL centrifuge tube, and obtaining an extracting solution to be detected for subsequent detection;
2. detecting the content of the drugs in the extract to be detected by double-antibody sandwich ELISA:
(1) coating: a murine anti-methamphetamine monoclonal antibody (commercially available product) was dissolved in 50mmol/L carbonate coating buffer (pH 9.6) to a final antibody concentration of 20. mu.g/mL, added to a 96-well plate at 100. mu.L/well, and allowed to stand overnight at 4 ℃.
(2) Discarding the coating buffer every other day, and using 1╳PBST solution washing 3 times, each time 3 min;
(3) the standards (700ng/mL, 350ng/mL, 175ng/mL, 87.5ng/mL, 43.75ng/mL, 21.88ng/mL, 10.94ng/mL) and the hair lysis supernatant were taken at different concentrations, added to a 96-well microplate at 100. mu.L/well, each set up a replicate, incubated at 37 ℃ for 1.5h, and incubated with 1×PBST solution, washing 3 times, each time 3 min.
(4) Mixing rabbit anti-methamphetamine polyclonal antibody (commercially available product) with primary anti-diluent (product model C01-04001, produced by Beijing Boaosen biotechnology Co., Ltd.) at a ratio of 1:1000 to obtain diluted rabbit anti-methamphetamine polyclonal antibody, and adding 100 μ L of the diluted rabbit anti-methamphetamine polyclonal antibody into each well of the ELISA plate obtained in step (3)The amphetamine polyclonal antibody is incubated for 1h at the constant temperature of 37 ℃ and then 1 is used×PBST solution washing 3 times, each time 3 min.
(5) Mixing goat anti-rabbit secondary IgG-HRP (a commercial product) with a secondary antibody diluent (product model C05-07002, produced by Beijing Boaosen biotechnology Co., Ltd.) at a ratio of 1:5000 to obtain diluted goat anti-rabbit secondary IgG-HRP, adding 100. mu.L of the diluted goat anti-rabbit secondary IgG-HRP into each hole of the ELISA plate obtained in the step (4), incubating at a constant temperature of 37 ℃ for 0.5h, and adding 1×PBST solution washing 4 times, each time 3 min.
(6) Taking substrate developing liquid A and B according to the ratio of 1:1 (two-component TMB color developing solution, product model PR1210, manufactured by Solarbio), 100. mu.L of Elisa stop solution (product model C1058, manufactured by Solarbio) is added into each hole of the ELISA plate after the step (5), incubation is carried out for 15-20min at constant temperature of 37 ℃, 50. mu.L of Elisa stop solution is added to stop the reaction, and the light absorption value is measured at 450nm within 30 min.
Experiment:
the hair with positive methamphetamine drugs and the hair with negative methamphetamine drugs (the known hair drugs are positive or negative before the experiment) are simultaneously used as the hair to be detected according to the detection method, and the detection results are shown in table 1.
TABLE 1 detection results of methamphetamine-type drugs in the hair to be detected
| Serial number | Sample name | OD450nm |
| 1 | Standard substance 1(700ng/mL) | 1.43 |
| 2 | Standard substance 2(350ng/mL) | 1.28 |
| 3 | Standard substance 3(175ng/mL) | 0.89 |
| 4 | Standard 4(87.50ng/mL) | 0.47 |
| 5 | Standard substance 5(43.75ng/mL) | 0.09 |
| 6 | Standard 6(21.88ng/mL) | 0.07 |
| 7 | Standard substance 6(10.94ng/mL) | 0.03 |
| 8 | Positive hair | 0.04 |
| 9 | Negative hair | 0.02 |
Calc was applied from the test results in Table 1The regression/fitting calculation program V0.2 gave a standard curve (as shown in FIG. 1, the abscissa of the graph indicates the concentration of the standard solution and the ordinate indicates the absorbance OD at 450nm450nm) And a calculation equation of formula (1):
y＝(A-D)/[1+(x/C)B]+D (1)
in the formula (1), y represents an absorbance value OD of the solution to be measured at 450nm450nmX is the content of methamphetamine drugs in the solution to be detected, A is 1.48701, B is-1.96812, C is 140.10735, and D is 0.01137;
r of the standard curve20.99729, the detection method provided by the invention is proved to have good specificity and high accuracy. According to the equation, the content of the methamphetamine drugs in the positive hair in the experiment is 19.09ng/mL, namely 0.32ng/mg, the content of the negative hair is 0-0.2ng/mg, and the requirement that the international detection threshold is 0.2ng/mg is met.
Although the present invention has been described in detail in the foregoing embodiments, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the invention.
Claims (10)
1. A detection kit for methamphetamine drugs in hair is characterized in that: the kit comprises an extracting solution, an enzyme label plate coated with a mouse anti-methamphetamine monoclonal antibody, a detection antibody and an enzyme-labeled secondary antibody, wherein the detection antibody is a rabbit anti-methamphetamine polyclonal antibody, and the enzyme-labeled secondary antibody is goat anti-rabbit IgG-HRP.
2. The kit for detecting methamphetamine-based drugs in hair according to claim 1, wherein: the extracting solution comprises a lysis solution, wherein the lysis solution consists of a NaOH solution with the concentration of 1mol/L and a SDS solution with the mass concentration of 10%.
3. The kit for detecting methamphetamine-based drugs in hair according to claim 2, wherein: the extracting solution also comprises a neutralizing solution, and the neutralizing solution is an acetic acid-sodium acetate buffer solution with the pH value of 9.6.
4. The kit for detecting methamphetamine-based drugs in hair according to claim 1, wherein: in the ELISA plate coated with the mouse anti-methamphetamine monoclonal antibody, the concentration of the mouse anti-methamphetamine monoclonal antibody is 20 mug/mL.
5. The kit for detecting methamphetamine-based drugs in hair according to claim 1, wherein: the detection antibody is a rabbit anti-methamphetamine polyclonal antibody diluted by 1:1000, and the enzyme-labeled secondary antibody is goat anti-rabbit IgG-HRP diluted by 1: 5000.
6. A method for detecting methamphetamine drugs in hair is characterized by comprising the following steps: the detection kit for methamphetamine-based narcotics in hair according to any one of claims 1-5, said detection method comprising the steps of:
s1, cutting the hair into small sections, placing the small sections in a container, and extracting the drugs by using the extracting solution to obtain the extracting solution to be detected;
s2, detecting the drug content in the extract to be detected by using a double-antibody sandwich ELISA method, which comprises the following steps:
s21, adding an extracting solution to be detected and a standard substance solution into an enzyme label plate coated with a mouse anti-methamphetamine monoclonal antibody according to 100 mu L/hole respectively, incubating at the constant temperature of 37 ℃ for 1.5h, and washing with a PBST solution for 3 times, wherein each time is 3 min; the standard solution is used for drawing a standard curve, and the concentrations of the standard solution are respectively as follows: 700ng/mL, 350ng/mL, 175ng/mL, 87.5ng/mL, 43.75ng/mL, 21.88ng/mL, 10.94 ng/mL;
s22, adding rabbit anti-methamphetamine polyclonal antibody diluted by 1:1000 into the holes of the ELISA plate treated in the step S21, adding 100 mu L of rabbit anti-methamphetamine polyclonal antibody into each hole, incubating at the constant temperature of 37 ℃ for 1h, and washing with PBST solution for 3 times, 3min each time;
s23, adding goat anti-rabbit IgG-HRP diluted by 1:5000 into the wells of the ELISA plate treated in the step S22, adding 100 mu L of the goat anti-rabbit IgG-HRP into each well, incubating at the constant temperature of 37 ℃ for 0.5h, and washing with PBST solution for 4 times, 3min each time;
s24, adding 100 mu L of substrate developing solution into each hole of the ELISA plate processed in the step 23, incubating for 15min-20min at constant temperature, adding 50 mu L of Elisa stop solution to stop reaction, and measuring the light absorption value at 450nm within 30 min.
7. The method for detecting methamphetamine-based drugs in hair according to claim 6, wherein: in step S1, the method for obtaining the extract to be tested includes: taking 50mg of hair to be detected, scrubbing the hair for 1-3 times by using 70% ethanol, cutting the hair into the size of 0.5-1 cm by using scissors, transferring the hair into a centrifuge tube, adding 500 mu L of lysate, incubating the hair for 10min at 75 ℃, transferring 300 mu L of cracked product into a new centrifuge tube, adding 200 mu L of neutralizing solution, standing the hair for 1min at room temperature, centrifuging the hair for 5min at 12000rpm, and absorbing supernatant to obtain the extracting solution to be detected; the lysis solution is formed by mixing NaOH solution with the concentration of 1mol/L and SDS solution with the mass concentration of 10%; the neutralizing solution is acetic acid-sodium acetate buffer solution with the pH value of 9.6.
8. The method for detecting methamphetamine-based drugs in hair according to claim 6, wherein: in step S21, the preparation process of the enzyme label plate coated with the mouse anti-methamphetamine monoclonal antibody comprises the following steps:
s211, dissolving a mouse anti-methamphetamine monoclonal antibody by using a carbonate coating buffer solution to enable the final concentration of the mouse anti-methamphetamine monoclonal antibody to be 20 mu g/mL, adding the mouse anti-methamphetamine monoclonal antibody into an enzyme label plate according to 100 mu L/hole, and standing overnight at 4 ℃;
s212, discarding the coating solution every other day, and washing with PBST solution for 3 times, each time for 3 min.
9. The method for detecting methamphetamine-based drugs in hair according to claim 8, wherein: in step S211, the concentration of the carbonate coating buffer is 50mmol/L, and the pH value is 9.6.
10. The method for detecting methamphetamine-based drugs in hair according to claim 6, wherein: in step S24, the substrate color developing solution is a two-component TMB color developing solution.
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| CN113848102A (en) * | 2021-09-27 | 2021-12-28 | 深圳市心月生物科技有限公司 | A kind of methamphetamine extract and preparation method thereof |
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