CN112608845A - Bionic stimulation device for mechanical pressure of stem cells - Google Patents
Bionic stimulation device for mechanical pressure of stem cells Download PDFInfo
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- CN112608845A CN112608845A CN202110030334.3A CN202110030334A CN112608845A CN 112608845 A CN112608845 A CN 112608845A CN 202110030334 A CN202110030334 A CN 202110030334A CN 112608845 A CN112608845 A CN 112608845A
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- 230000000638 stimulation Effects 0.000 title claims abstract description 43
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 20
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 17
- 210000004027 cell Anatomy 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000009434 installation Methods 0.000 claims abstract description 8
- 238000001125 extrusion Methods 0.000 claims description 24
- 238000000926 separation method Methods 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 230000009471 action Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
本发明涉及干细胞压力刺激方法领域，具体为一种干细胞机械压应力仿生刺激装置。解决了现有力学刺激方向单一性和刺激效率低的问题的问题。包括：整个装置以箱体为安装主体，所述箱体内部安装有一级离心器，一级离心器带动其上安装的若干二级离心器同步旋转，二级离心器带动其上安装的挤压组旋转，挤压组内液体袋通过离心力挤压细胞盘。装置采用双离心设备，通过离心力使得液体袋对细胞盘进行多方向挤压，实现力学刺激的万向性，提高研究的准确性，同时可一次性实现大批量细胞刺激。
The invention relates to the field of stem cell pressure stimulation methods, in particular to a stem cell mechanical pressure stress bionic stimulation device. The problems of the singleness of the existing mechanical stimulation direction and the low stimulation efficiency are solved. Including: the whole device takes the box body as the installation main body, the first stage centrifuge is installed inside the box body, the first stage centrifuge drives several second stage centrifuges installed on it to rotate synchronously, and the second stage centrifuge drives the squeezers installed on it to rotate. The group is rotated, and the liquid bag in the squeeze group squeezes the cell disk by centrifugal force. The device adopts double centrifugation equipment, and the liquid bag squeezes the cell disc in multiple directions through centrifugal force, which realizes the universality of mechanical stimulation, improves the accuracy of research, and can achieve large-scale cell stimulation at one time.
Description
Technical Field
The invention relates to the field of stem cell pressure stimulation methods, in particular to a mechanical pressure stress bionic stimulation device for stem cells.
Background
Cells in a living body are in an environment full of various mechanical stimuli, the cells are stretched, contracted, twisted and the like by skeletal motion during motion, the digestive tract wriggles during eating, the shearing force caused by body fluid flow and the like, and even a simple finger-operated motion can bring the mechanical stimuli to the cells in the living body. As is known to all, exercise is beneficial to health, in vivo, normal biomechanical stimulation is also necessary for normal physiological processes such as bone tissue balance, embryonic development and the like, and research shows that different mechanical stimulation can have great influence on life activities such as growth, proliferation, differentiation and the like of cells, and abnormal biomechanical stimulation can also generate diseases such as osteoporosis and the like.
Tension and compression are the most common mechanical stimuli in vivo, and numerous researchers have been added to in vitro simulation studies of mechanical stimuli in cells in vivo for many years. The current research on the action of cells under tensile and compressive mechanical stimulation mainly includes two aspects: one is mechanical stimulation under two-dimensional culture conditions, which comprises two-point mechanical stretching, four-point mechanical stretching, pneumatic mechanical action and the like on an elastic membrane, and the mechanical stimulation is generated on cells cultured on the elastic membrane through the elastic action of the membrane, and the greatest defect of the loading mode is that the deformation of each part on the elastic membrane is not uniform; one is mechanical stimulation under three-dimensional culture conditions, including mechanical stimulation under fixed deformation, namely, research on the influence of fixed stress or strain conditions on cell culture, and the research belongs to cell culture research under static mechanical stimulation. The cell culture research under dynamic mechanical stimulation is mechanical stimulation realized by using a piezoelectric ceramic piece, a lead screw, a connecting rod mechanism and the like, but each device cannot realize mechanical stimulation in all directions, so that the inaccuracy of research data is easily caused. Meanwhile, the existing mechanical stimulation device has small one-time stimulation cell amount and low stimulation efficiency.
Disclosure of Invention
The invention provides a mechanical compressive stress bionic stimulation device for stem cells, which effectively solves the problems of single direction and low stimulation efficiency of the existing mechanical stimulation.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a bionic stimulation device for mechanical pressure stress of stem cells takes a box body 1 as an installation main body, and is characterized in that a primary centrifuge 2 is installed inside the box body 1, the primary centrifuge 2 drives a plurality of secondary centrifuges 3 installed on the primary centrifuge to rotate, the secondary centrifuges 3 drive an extrusion group 4 installed on the secondary centrifuges to rotate, and a liquid bag 22 in the extrusion group 4 extrudes a cell plate 23 through centrifugal force.
The primary centrifuge 2 drives the fluted disc 8 to rotate through the primary motor 5 and the rotating rod 6.
Fluted disc 8 is movably installed on box 1 through one-level main shaft 7, is provided with spliced pole 9 and carousel 10 simultaneously on fluted disc 8.
The second-stage centrifuge 3 is mounted on the turntable 10.
The secondary centrifuge 3 takes a secondary box body 11 as an installation main body, a matching rotating device of a worm wheel 13 and a worm 14 is installed in the secondary box body 11, and the worm 14 is driven by a secondary motor 15; the worm wheel 13 is arranged on the secondary main shaft 12, one end of the secondary main shaft 12 is movably arranged on the secondary box body 11, the other end is provided with a main gear 16, the main gear 16 is mutually meshed with a driven gear 19 arranged on a driven shaft 18,
the driven shaft 18 is provided with the extrusion group 4.
The secondary main shaft 12 is stabilized by the aid of a mounting frame 17.
A plurality of squeezing boxes 20 are arranged in the squeezing group 4, and a liquid bag 22 and a cell tray 23 are arranged in each squeezing box 20.
The squeeze box 20 is provided with a bulkhead 21 between a fluid bag 22 and a cell tray 23.
The primary motor 5 and the secondary motor 1 are controlled by a controller 24.
The invention has the beneficial effects that: 1) this device is through the cooperation that adopts one-level centrifuge and second grade centrifuge, on the rotatory basis of one-level centrifuge, drives the rotation of second grade centrifuge for extrusion group on the second grade centrifuge is linear extrusion cell dish.
2) This device is through the cooperation of two centrifuges for the extrusion cell dish that the liquid bag can be multidirectional realizes multidirectional mechanics stimulation.
3) The extrusion group of the device is provided with a plurality of extrusion groups, and each extrusion group is internally provided with a plurality of extrusion discs, so that mechanical stimulation can be performed on a large number of stem cells at one time.
Drawings
FIG. 1 is a schematic view of the outer structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic view of a one-stage centrifuge mounting arrangement;
FIG. 4 is a schematic view of a two-stage centrifuge mounting arrangement;
FIG. 5 is a schematic view of a worm gear mounting arrangement;
FIG. 6 is a schematic view of a press set configuration;
FIG. 7 is a schematic view of a crush box construction;
shown in the figure: the device comprises a box body 1, a primary centrifuge 2, a secondary centrifuge 3, an extrusion group 4, a primary motor 5, a rotating rod 6, a primary main shaft 7, a fluted disc 8, a connecting column 9, a rotary disc 10, a secondary box body 11, a secondary main shaft 12, a worm wheel 13, a worm 14, a secondary motor 15, a main gear 16, an installation frame 17, a driven shaft 18, a driven gear 19, an extrusion box 20, a partition frame 21, a liquid bag 22, a cell disc 23 and a controller 24.
Detailed Description
The technical scheme of the invention is further explained by specific embodiments in the following with the accompanying drawings:
example one
Referring to the attached drawings 1-7, the invention aims to provide a mechanical compressive stress bionic stimulation device for stem cells, which realizes the functions of multidirectional force and linear pressurization. The multidirectional force and the linear pressurization are realized by the cooperation of double centrifugal devices, and the specific structure is as follows.
Whole device uses box 1 as installation main part 1, has one-level centrifuge 2 and second grade centrifuge 3 at box 1 internally mounted, and through the double-rotation cooperation of one-level centrifuge 2 and second grade centrifuge 3, the extrusion group 4 work of installation on the drive second grade centrifuge 3 realizes the multidirectional linear mechanics mechanical extrusion to cell dish 23.
As shown in fig. 3, the mounting structure of the primary centrifuge 2 is such that the primary centrifuge 2 adopts the cooperative rotation of the rotating rod 6 and the toothed disc 8. The rotary rod 6 is driven to rotate by a primary motor 5, and the primary motor 5 is mounted on the box body 1. Meanwhile, a rotatable primary main shaft 7 is installed on the box body 1, and a fluted disc 8 is installed through the primary main shaft 7. The teeth of the fluted disc 8 are horizontal teeth, and a connecting column 9 is fixedly arranged on the fluted disc 8. A rotary disc 10 is fixedly arranged on the connecting column 9, and a secondary centrifuge 3 is arranged on the rotary disc 10.
During the rotation of the rotating disk 10, the secondary centrifuge 3 is synchronously driven to rotate.
The structure of the secondary centrifuge 3 is shown in fig. 4, on which a secondary box 11 is mounted, and a matching rotating structure of a worm wheel 13 and a worm 14 is mounted in the secondary box 11. The worm 14 is driven to rotate by a secondary motor 15, and the worm wheel 13 is mounted in the secondary housing 11 via a secondary spindle 12. While a main gear 16 is mounted on the upper end of the secondary main shaft 12. A plurality of movable driven shafts 18 are arranged on the rotary table 10 outside the secondary box body 11. In order to ensure the stability of the driven shaft 18, the device is provided with a mounting frame 17 on the turntable 10 to assist in stabilizing the driven shaft 18.
A driven gear 19 on the driven shaft 18 is meshed with the main gear 16 to realize the rotation of the driven shaft 18. The driven shaft 18 is provided with a pressing group 4.
The structure of the extrusion group 4 is shown in fig. 6, and four groups of extrusion boxes are arranged inside the extrusion group. The squeeze box 20 is constructed as shown in FIG. 7, and the squeeze box 20 is divided into two spaces by a partition 21, in one of which a fluid bag 22 is placed and in the other of which a cell dish 23 is placed. The fluid bag 22 is made of soft material, and its shape can be changed during the centrifugation process. The liquid bag 22 generates multi-directional uneven and linear mechanical stimulation to the cell plate 23 through the action of centrifugal force.
Meanwhile, a controller 24 is arranged on the box body 1, and the rotation rates of the primary motor 5 and the secondary motor 15 are controlled through the controller 24, so that the centrifugal force is controlled.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A bionic stimulation device for mechanical pressure stress of stem cells takes a box body as an installation main body, and is characterized in that a primary centrifuge is installed in the box body and drives a plurality of secondary centrifuges installed on the primary centrifuge to synchronously rotate, the secondary centrifuges drive an extrusion group installed on the secondary centrifuges to rotate, and a liquid bag in the extrusion group extrudes a cell tray through centrifugal force.
2. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: the first-stage centrifuge drives the fluted disc to rotate through the first-stage motor and the rotating rod.
3. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 2, characterized in that: the fluted disc is movably installed on the box body through the primary main shaft, and a connecting column and a turntable are arranged on the fluted disc.
4. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 3, characterized in that: and a secondary centrifuge is arranged on the rotary disc.
5. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: the secondary centrifuge takes a secondary box body as an installation main body, a worm wheel and worm matched rotating device is installed in the secondary box body, and the worm is driven by a secondary motor; the worm wheel is installed on the second grade main shaft, and second grade main shaft one end is mobilizable to be installed on the second grade box, and the master gear is installed to the other end, master gear and the driven gear intermeshing who installs on the driven shaft.
6. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 5, characterized in that: and the driven shaft is provided with an extrusion group.
7. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 5, characterized in that: the secondary main shaft is assisted and stabilized through a mounting frame.
8. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: a plurality of extrusion boxes are arranged in the extrusion group, and liquid bags and cell trays are arranged in the extrusion boxes.
9. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 8, characterized in that: the extrusion box is provided with a separation frame between the liquid bag and the cell tray.
10. The bionic stimulation device for mechanical pressure stress of stem cells according to claim 1, characterized in that: and the box body is provided with a controller for controlling the primary motor and the secondary motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110030334.3A CN112608845B (en) | 2021-01-11 | 2021-01-11 | Stem cell mechanical pressure bionic stimulation device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110030334.3A CN112608845B (en) | 2021-01-11 | 2021-01-11 | Stem cell mechanical pressure bionic stimulation device |
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| CN112608845A true CN112608845A (en) | 2021-04-06 |
| CN112608845B CN112608845B (en) | 2023-07-25 |
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