CN112596250A - Illumination system of flow cytometry sorter and flow cytometry sorter - Google Patents

Abstract

The invention discloses an illumination system of a flow cytometer and a flow cytometer, wherein the illumination system of the flow cytometer includes a light source unit and a beam shaping unit, and the beam shaping unit is located in the on the propagation path of the outgoing light of the light source unit; the beam shaping unit includes a cylindrical aspheric lens group, and the cylindrical aspheric lens group includes a first conical surface and a second conical surface; the first conical surface Has the first optical power

The second conical surface has a second optical power

in

By adopting the above technical solution, by setting the cylindrical aspheric lens group to include two conical surfaces, and by reasonably setting the optical power of the two conical surfaces, it can be ensured that the light beam emitted by the light source unit can obtain a flat-top elliptical beam after passing through the beam shaping unit, and The flat-top elliptical beam has a large spot area, which ensures high detection accuracy of cells or particles; at the same time, it can ensure that the energy utilization rate of the outgoing laser is high.

Description

Illumination system of a flow cytometer and flow cytometer

technical field

Embodiments of the present invention relate to the technical field of cell sorting, and in particular, to an illumination system of a flow cytometer and a flow cytometer.

Background technique

Flow cytometry-based instruments, including flow cytometers, blood analyzers, particle analyzers, etc., are technical platforms that perform rapid quantitative analysis and sorting of cells or other particles arranged in a single column in the target stream one by one. The basic principle is to use a focused laser beam to irradiate a single cell or particle, and at the same time use a photodetector to analyze the generated scattered light or fluorescent signal to obtain various parameters of the object to be detected. Among them, the laser light source and its optical system are one of the core components of the flow cytometer. The quality and stability of the focused beam directly determine the performance index of the flow cytometer. Since the cell sample to be analyzed flows at a high speed (5000 cells/second) in the target stream, the time to pass through the laser irradiation area is only in the order of microseconds, and the intensity of the scattered light or fluorescence signal generated by the laser irradiation area is closely related to the optical power density distribution of the laser irradiation area. related.

In order to ensure that each cell or particle passing through the focused spot receives the same intensity of laser irradiation, and to ensure the analysis accuracy of the flow cytometer, it is necessary to ensure that the size of the focused spot is larger. However, in the prior art, either by increasing the spot size of the incident beam, or by adjusting the structure of the laser shaping device to increase the size of the focused spot. However, increasing the spot size of the incident beam has higher requirements on the laser and increases the cost of the laser; adjusting the structure of the laser shaping device will make it difficult to process the laser shaping device, and the flat-top portion of the shaped flat-top beam accounts for a small proportion of the laser energy utilization. rate is low.

SUMMARY OF THE INVENTION

In view of this, embodiments of the present invention provide an illumination system for a flow cytometer and a flow cytometer. The flow cytometer has a simple structure and a large flat-top beam size.

In a first aspect, an embodiment of the present invention provides an illumination system for a flow cytometer, including a light source unit and a beam shaping unit, wherein the beam shaping unit is located on a propagation path of outgoing light from the light source unit;

The beam shaping unit includes a cylindrical aspheric lens group, and the cylindrical aspheric lens group includes a first conical surface and a second conical surface;

所述第二圆锥面具备第二光焦度

其中

The first conical surface has a first optical power

The second conical surface has a second optical power

in

Optionally, the radius of curvature of the first conical surface is R _y1 , the conic coefficient of the first conical surface is k _y1 , and |R _y1 |<100mm, -1000<k _y1 <-1;

The radius of curvature of the second conical surface is R _y2 , the conic coefficient of the second conical surface is k _y2 , and |R _y2 |<100mm, -1000<k _y2 <-1.

Optionally, the focal length f1 of the first conical surface satisfies 0mm<f1<250mm, and the focal length f2 of the second conical surface satisfies -250mm<f2<0mm.

Optionally, the cylindrical aspheric lens group includes a first cylindrical aspheric lens and a second cylindrical aspheric lens, the first cylindrical aspheric lens includes the first conical surface, and the second cylindrical aspheric lens. The cylindrical aspheric lens includes the second conical surface;

The first cylindrical aspheric lens is located on the propagation path of the outgoing light from the light source unit, and the second cylindrical aspheric lens is located on the propagation path of the transmitted light transmitted by the first cylindrical aspheric lens Or, the second cylindrical aspheric lens is located on the propagation path of the outgoing light of the light source unit, and the first cylindrical aspheric lens is located in the transmission obtained by the second cylindrical aspheric lens. on the propagation path of the light.

Optionally, the cylindrical aspheric lens group includes a third cylindrical aspheric lens, and the third cylindrical aspheric lens includes the first conical surface and the second conical surface;

The first conical surface is located on the propagation path of the outgoing light from the light source unit, and the second conical surface is located on the propagation path of the transmitted light obtained through the first conical surface; or, the second cone The surface is located on the propagation path of the outgoing light from the light source unit, and the first conical surface is located on the propagation path of the transmitted light transmitted through the first conical surface.

Optionally, after the light emitted from the light source unit passes through the beam shaping unit, a flat-top elliptical beam is obtained;

along the long axis direction of the flat-top elliptical beam, the light intensity of the flat-top elliptical beam is uniformly distributed; along the short-axis direction of the flat-top elliptical beam, the light intensity of the flat-top elliptical beam is Gaussian distribution;

The spot size of the flat-top elliptical beam in the direction of the long axis is the first spot size, where the first spot size Wx is the beam diameter corresponding to the light intensity I _w1 , where I _w1 is the long axis 1/e ² times the maximum light intensity in the direction;

The second spot size Wt is the beam diameter corresponding to when the light intensity uniformity satisfies a preset value, wherein the preset value a satisfies 95%≤a≤100%, and Wt/Wx>75%.

Optionally, the light source unit includes a multi-wavelength light source unit.

Optionally, the multi-wavelength light source unit includes a plurality of single-mode fiber-coupled lasers;

The illumination system further includes a plurality of collimating lenses and a plurality of beam turning devices, the collimating lenses are in one-to-one correspondence with the single-mode fiber-coupled lasers, and the beam turning devices are in one-to-one correspondence with the collimating lenses;

The collimating lens is located on the propagation path of the outgoing laser light of the single-mode fiber-coupled laser, and is used for collimating the outgoing laser light to obtain a collimated laser beam;

The beam turning device is located on the propagation path of the collimated laser beam, and is used for reflecting the collimated laser beam to the geometric center of the cylindrical aspheric lens group.

Optionally, the illumination system further includes an achromatic doublet lens, and the achromatic doublet lens is located on the propagation path of the transmitted light obtained through the cylindrical aspheric lens group;

The focal length f3 of the achromatic doublet lens satisfies 20mm<f2<100mm.

In a second aspect, an embodiment of the present invention further provides a flow cytometer, including the illumination system of the flow cytometer according to the first aspect.

第二圆锥面具备第二光焦度

其中

如此不仅可以保证照明系统中柱面非球面透镜组结构简单，保证柱面非球面透镜组加工工艺简单；同时，本发明实施例提供的照明系统可以得到平顶椭圆光束，且平顶椭圆光束的光斑面积较大，保证流动室内的细胞或微粒在该较大的平顶椭圆光束的辐照下产生的散射光或荧光信号强度相同，保证细胞或微粒检测精确度高；同时可以保证出射激光的能量利用率较高。The illumination system of the flow cytometry sorter and the flow cytometry sorter provided by the embodiments of the present invention are provided by setting the illumination system of the flow cytometry sorter to include a light source unit and a beam shaping unit, and at the same time, the beam shaping unit is arranged to include a cylindrical surface Aspherical lens group, the cylindrical aspherical lens group includes a first conical surface and a second conical surface, and the first conical surface has a first optical power

The second conical surface has the second optical power

in

This can not only ensure that the cylindrical aspheric lens group in the lighting system has a simple structure, but also ensure that the processing technology of the cylindrical aspheric lens group is simple; at the same time, the lighting system provided by the embodiment of the present invention can obtain a flat-top elliptical light beam, and the flat-top elliptical light beam can be obtained. The large spot area ensures that cells or particles in the flow chamber generate the same intensity of scattered light or fluorescent signals under the irradiation of the larger flat-top elliptical beam, ensuring high detection accuracy of cells or particles; The energy utilization rate is high.

Description of drawings

Other features, objects and advantages of the present invention will become more apparent upon reading the detailed description of non-limiting embodiments made with reference to the following drawings:

1 is a schematic structural diagram of an illumination system of a flow cytometer according to an embodiment of the present invention;

2 is a schematic cross-sectional view of an outgoing light beam and a schematic view of light intensity distribution provided by an embodiment of the present invention;

3 is a schematic cross-sectional view of a flat-top elliptical beam provided by an embodiment of the present invention and a schematic view of light intensity divisions;

Fig. 4 is the enlarged schematic diagram of A area in Fig. 3;

5 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention;

6 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention;

7 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention;

8 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention;

FIG. 9 is a schematic diagram of a plurality of flat-top elliptical light beams provided by an embodiment of the present invention.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be completely described below with reference to the accompanying drawings in the embodiments of the present invention and through specific implementation manners. Obviously, the described embodiments are a part of the embodiments of the present invention, rather than all the embodiments, based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work, All fall within the protection scope of the present invention.

第二圆锥面201b具备第二光焦度

其中

FIG. 1 is a schematic structural diagram of an illumination system of a flow cytometry sorter provided by an embodiment of the present invention. As shown in FIG. 1 , the illumination system of the flow cytometry sorter provided by an embodiment of the present invention includes a light source unit 10 and a The beam shaping unit 20, the beam shaping unit 20 is located on the propagation path of the outgoing light of the light source unit 10; the beam shaping unit 20 includes a cylindrical aspheric lens group 201, and the cylindrical aspheric lens group 201 includes a first conical surface 201a and a second Conical surface 201b; the first conical surface 201a has a first optical power

The second conical surface 201b has the second optical power

in

Exemplarily, as shown in FIG. 1 , the outgoing light beam 101 of the light source unit 10 is irradiated onto the flow chamber 40 after passing through the beam shaping unit 20 , and converges at the center of the flow chamber 40 . The outgoing light beam 101 may be a collimated Gaussian spot, and a schematic cross-sectional diagram and a schematic diagram of light intensity distribution are shown in FIG. 2 . Wherein I ₁ is the light intensity at the center of the light spot, that is, the maximum light intensity of the outgoing beam 101 , and I ₂ is 1/e ² times of I ₁ . W ₁ is the beam diameter corresponding to the light intensity of I ₂ . In the present invention, W ₁ may be any value between 0.5 mm and 5 mm, which is not limited in the embodiment of the present invention. Further, the light source unit 10 may be a single-wavelength light source unit or a multi-wavelength light source unit, which is not limited in this embodiment of the present invention.

满足

第二圆锥面201b的第二光焦度

满足

如此可以保证出射激光101经柱面非球面透镜组201后为平顶椭圆光束，且该平顶椭圆光束具备较大的光斑，保证流动室40内的细胞或微粒在该较大的平顶椭圆光束的辐照下产生的散射光或荧光信号强度相同，保证细胞或微粒检测精确度高。The cylindrical aspheric lens group 201 includes a first conical surface 201a and a second conical surface 201b, and the first conical surface 201a has a positive refractive power, and the second conical surface 201b has a negative refractive power, that is, the first conical surface 1st optical power of 201a

Satisfy

The second optical power of the second conical surface 201b

Satisfy

In this way, it can be ensured that the outgoing laser 101 is a flat-top elliptical beam after passing through the cylindrical aspheric lens group 201, and the flat-top elliptical beam has a larger light spot, ensuring that the cells or particles in the flow chamber 40 are in the larger flat-top ellipse. The intensity of scattered light or fluorescent signal generated under the irradiation of the beam is the same, which ensures high detection accuracy of cells or particles.

Optionally, the cylindrical aspheric lens group 201 may include one or more cylindrical aspheric lenses, which is not limited in this embodiment of the present invention, and only needs to ensure that the cylindrical aspheric lens group 201 includes two lenses with opposite refractive powers. A conical surface is sufficient. Further, the cylindrical aspheric lens may be a Powell prism, or may be a shaping lens in other forms, which is not limited in this embodiment of the present invention.

To sum up, in the illumination system for flow cytometric sorting provided by the embodiment of the present invention, the beam shaping unit is arranged to include two conical surfaces, and the optical powers of the two conical surfaces are opposite to ensure that the The light spot of the flat-top elliptical beam is larger, ensuring that the scattered light or fluorescence signal intensity generated by the cells or particles in the flow chamber under the irradiation of the larger flat-top elliptical beam is the same, ensuring high detection accuracy of cells or particles; It can ensure that the energy utilization rate of the outgoing laser is relatively high; in addition, the lighting system provided by the embodiment of the present invention has a simple structure, and only by setting the beam shaping unit to include two conical surfaces with opposite focal powers, it can be ensured that a flat-top ellipse with a larger light spot can be obtained. The manufacturing process of the beam shaping unit is simple, the manufacturing yield of the beam shaping unit is improved, and the manufacturing cost of the beam shaping unit is reduced.

Specifically, FIG. 3 is a schematic cross-sectional view and a schematic view of light intensity divisions of a flat-top elliptical beam provided by an embodiment of the present invention, and FIG. 4 is an enlarged schematic view of the area A in FIG. 3 . As shown in FIGS. 3 and 4 , the light source unit 10 The outgoing ray 101 of the beam is obtained by the beam shaping unit 20 to obtain a flat-top elliptical beam; along the long-axis direction of the flat-top elliptical beam, as shown in the X direction in the figure, the light intensity of the flat-top elliptical beam is uniformly distributed; along the flat-top elliptical beam In the short-axis direction, as shown in the Y direction in the figure, the light intensity Gaussian distribution of the flat-top elliptical beam; the spot size of the flat-top elliptical beam in the long-axis direction is the first spot size, where the first spot size W _x is the beam diameter corresponding to the light intensity I _w1 , where I _w1 is 1/e ² times the maximum light intensity in the long axis direction; the second spot size Wt is the corresponding beam diameter when the light intensity uniformity meets the preset value, wherein , the preset value a satisfies 95%≤a≤100%, and Wt/Wx>75%; wherein, the calculation formula of the light intensity uniformity U is as follows:

I _max and I _min respectively represent the maximum light intensity value and the minimum light intensity value of the flat-top elliptical beam in the direction of the long axis.

Exemplarily, as shown in FIG. 3 and FIG. 4 , after the outgoing beam 101 passes through the beam shaping unit 20, a flat-top elliptical light beam is obtained, and the flat-top elliptical light beam is uniformly distributed in the long-axis direction, that is, the X direction shown in the figures, It is ensured that the scattered light or fluorescence signal intensity generated by the cells or particles in the flow chamber under the irradiation of the larger flat-top elliptical beam is the same, and the detection accuracy of the cells or particles is guaranteed to be high.

Further, in the embodiment of the present invention, the second spot size Wt is the beam diameter corresponding to when the light intensity uniformity satisfies a preset value, wherein the preset value a can satisfy 95%≤a≤100%, that is, the uniformity is greater than 95%. % The ratio of the corresponding beam diameter Wt to the beam diameter Wx of the flat-top elliptical beam in the long-axis direction is greater than 75%, as shown in Figure 3 and Figure 4, which fully ensures that the flat-top elliptical beam has good uniformity in the long-axis direction. This ensures that the cells or particles in the flow chamber generate the same intensity of scattered light or fluorescent signals under the irradiation of the larger flat-top elliptical beam, thereby ensuring high detection accuracy of cells or particles. Further, the preset value in the embodiment of the present invention can even reach 98%, which fully ensures that the flat-top elliptical beam has good uniformity in the long axis direction.

Exemplarily, in this embodiment of the present invention, the beam diameter Wx of the flat-top elliptical beam in the long axis direction may be 60 μm, and the Wt may be greater than 45 μm. Further, the beam diameter Wy of the flat-top elliptical beam in the short-axis direction may be 6-13 μm. Further, when the uniformity satisfies the preset value, the corresponding beam diameter Wt may be any value between 20 and 60 μm, which is not limited in the embodiment of the present invention, and is only exemplified that Wt may be greater than 45 μm.

Optionally, the surface shape function of the first conical surface 201a may satisfy:

R_y1为第一圆锥面201a的曲率半径，k_y1为第一圆锥面201a的圆锥系数，且|R_y1|<100mm，-1000<k_y1<-1；in,

R _y1 is the radius of curvature of the first conical surface 201a, k _y1 is the conic coefficient of the first conical surface 201a, and |R _y1 |<100mm, -1000<k _y1 <-1;

The surface shape function of the second conical surface 201b can satisfy:

R_y2为第二圆锥面201b的曲率半径，k_y2为第二圆锥面201b的圆锥系数，且|R_y2|<100mm，-1000<k_y2<-1。in,

R _y2 is the radius of curvature of the second conical surface 201b, k _y2 is the conic coefficient of the second conical surface 201b, and |R _y2 |<100 mm, −1000<k _y2 <−1.

It can be understood that, according to the surface shape function of the first conical surface 201a and the surface shape function of the second conical surface 201b, it can be known that the surface shape functions of the first conical surface 201a and the second conical surface 201b are only related to a single direction (y direction). ) is related to the variables on ), so the surface function of the first conical surface 201a and the second conical surface 201b is simple, the structure of the first conical surface 201a and the second conical surface 201b is simple, and the preparation process is simple, which can ensure the 201a and the second conical surface 201b. The preparation efficiency and preparation yield of 201b are high.

It should be noted that the above-mentioned embodiment only takes a feasible surface shape function of the first conical surface 201a and the second conical surface 201b as an example for description. It can be understood that the surface shape function of the first conical surface 201a is also Other function expressions can be satisfied, for example:

Wherein, at least one of a1, a2, a3, a4, a5, a6, a7 and a8 is not zero.

The surface shape function of the second conical surface 201b can also satisfy other functional expressions, for example:

Wherein, at least one of a1', a2', a3', a4', a5', a6', a7' and a8' is not zero.

In this way, the surface shape functions of the first conical surface 201a and the second conical surface 201b are also only related to the variables in a single direction (y direction), so the surface shape functions of the first conical surface 201a and the second conical surface 201b are simple, and the same It can ensure that the first conical surface 201a and the second conical surface 201b have a simple structure and a simple manufacturing process, and can ensure that the manufacturing efficiency and the manufacturing yield of the 201a and the second conical surface 201b are high. Further, the radius of curvature and the conic coefficient of the first conical surface 201a and the radius of curvature and conic coefficient of the second conical surface 201b are reasonably set to ensure that the light spot of the flat-top elliptical beam obtained by the beam shaping unit 20 is larger, ensuring that cells Or the particle detection accuracy is high and the energy utilization rate of the outgoing laser is high.

Optionally, the focal length f1 of the first conical surface 201a may satisfy 0 mm<f1<250 mm, and the focal length f2 of the second conical surface 201b may satisfy -250 mm<f2<0 mm. Reasonable setting of the focal length of the first conical surface 201a and the focal length of the second conical surface 201b can ensure that the light spot of the flat-top elliptical beam obtained by the beam shaping unit 20 is larger, and the detection accuracy of cells or particles is high and the energy of the outgoing laser can be ensured Utilization is high.

Optionally, the cylindrical aspheric lens group 201 may include one or more cylindrical aspheric lenses, and the first conical surface 201a and the second conical surface 201b may be respectively disposed on the same cylindrical aspheric lens, or disposed on the same cylindrical aspheric lens. Different cylindrical aspheric lenses will be described in detail below for different situations.

First, the description will be given by taking the example that the cylindrical aspheric lens group 201 includes two cylindrical aspheric lenses.

Optionally, as shown in FIG. 1, the cylindrical aspheric lens group 201 includes a first cylindrical aspheric lens 2011 and a second cylindrical aspheric lens 2012, and the first cylindrical aspheric lens 2011 includes a first conical surface. 201a, the second cylindrical aspherical lens 2012 includes a second conical surface 201b; the first cylindrical aspherical lens 2011 is located on the propagation path of the outgoing light 101 of the light source unit 10, and the second cylindrical aspherical lens 2012 is located through the first On the propagation path of the transmitted light obtained by the cylindrical aspheric lens 2011. In this way, the outgoing beam 101 passes through the first cylindrical aspheric lens 2011 and the second cylindrical aspheric lens 2012 in turn to obtain a flat-top elliptical beam, and the light spot of the flat-top elliptical beam is large, ensuring that the cells or particles in the flow chamber are in the relatively large area. The intensity of scattered light or fluorescence signal generated by the irradiation of the large flat-top elliptical beam is the same, which ensures high detection accuracy of cells or particles; meanwhile, it can ensure that the energy utilization rate of the outgoing laser is high.

Optionally, FIG. 5 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention. As shown in FIG. 5 , the cylindrical aspheric lens group 201 includes a first cylindrical aspheric lens. 2011 and the second cylindrical aspheric lens 2012, the first cylindrical aspheric lens 2011 includes a first conical surface 201a, the second cylindrical aspheric lens 2012 includes a second conical surface 201b; the second cylindrical aspheric lens 2012 is located at On the propagation path of the outgoing light from the light source unit 10 , the first cylindrical aspheric lens 2011 is located on the propagation path of the transmitted light transmitted by the second cylindrical aspheric lens 2012 . In this way, the outgoing beam 101 passes through the second cylindrical aspheric lens 2012 and the first cylindrical aspheric lens 2011 in turn to obtain a flat-top elliptical beam, and the flat-top elliptical beam has a larger light spot to ensure that cells or particles in the flow chamber are in the relatively large area. The intensity of scattered light or fluorescence signal generated by the irradiation of the large flat-top elliptical beam is the same, which ensures high detection accuracy of cells or particles; meanwhile, it can ensure that the energy utilization rate of the outgoing laser is high.

The case where the cylindrical aspherical lens group 201 includes two cylindrical aspherical lenses has been described above. Next, the case where the cylindrical aspherical lens group 201 includes one cylindrical aspherical lens will be described.

Optionally, FIG. 6 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention. As shown in FIG. 6 , the cylindrical aspheric lens group 201 includes a third cylindrical aspheric lens. In 2013, the third cylindrical aspherical lens 2013 includes a first conical surface 201a and a second conical surface 201b; the first conical surface 201a is located on the propagation path of the outgoing light of the light source unit 10, and the second conical surface 201b is located through the first conical surface 201b. On the propagation path of the transmitted light obtained by transmitting the surface 201a. In this way, the outgoing light beam 101 passes through the first conical surface 201a and the second conical surface 201b in turn to obtain a flat-topped elliptical beam, and the flat-topped elliptical beam has a larger light spot, ensuring that cells or particles in the flow chamber are in the larger flat-topped elliptical beam. The intensity of scattered light or fluorescent signal generated under the irradiation of the beam is the same, which ensures high detection accuracy of cells or particles; meanwhile, it can ensure that the energy utilization rate of the outgoing laser is high.

Optionally, FIG. 7 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention. As shown in the figure, the cylindrical aspheric lens group 201 includes a third cylindrical aspheric lens 2013 , the third cylindrical aspheric lens 2013 includes a first conical surface 201a and a second conical surface 201b; the second conical surface 201b is located on the propagation path of the outgoing light from the light source unit 10, and the first conical surface 201a is located through the second conical surface 201b on the propagation path of the transmitted light obtained by the transmission. In this way, the outgoing beam 101 passes through the second conical surface 201b and the first conical surface 201a in turn to obtain a flat-topped elliptical beam, and the flat-topped elliptical beam has a larger light spot to ensure that cells or particles in the flow chamber are in the larger flat-topped elliptical beam. The intensity of scattered light or fluorescent signal generated under the irradiation of the beam is the same, which ensures high detection accuracy of cells or particles; meanwhile, it can ensure that the energy utilization rate of the outgoing laser is high.

The above embodiments have described in detail the different composition modes of the cylindrical aspheric lens group 201. It can be understood that the embodiment of the present invention does not limit the specific composition mode of the cylindrical aspheric lens group 201. The group 201 may include two cylindrical aspheric lenses, as shown in FIG. 1 and FIG. 5 , so as to ensure that each cylindrical aspheric lens has a simple structure and a simple manufacturing process; or, the cylindrical aspheric lens group 201 may also include A cylindrical aspheric lens, as shown in FIG. 6 and FIG. 7 , can ensure that the overall structure of the cylindrical aspheric lens group 201 is simple and compact, and has a high degree of integration. Further, in this embodiment of the present invention, the outgoing light beam 101 may first pass through the first conical surface 201a and then pass through the second conical surface 201b, as shown in FIG. 1 and FIG. 6 ; alternatively, the outgoing light beam 101 may first pass through the second conical surface 201b then passes through the first conical surface 201a, as shown in FIG. 5 and FIG. 7 , this is not limited in this embodiment of the present invention, it only needs to ensure that the outgoing light beam 101 can pass through the first conical surface 201a and the second conical surface 201b, After the first conical surface 201a and the second conical surface 201b are shaped, a flat-top elliptical beam with a larger spot can be obtained.

Further, the light source unit 10 may include a multi-wavelength light source unit 11, which ensures that the wavelength range of the light source unit 10 is relatively large, for example, covers the entire visible light range, and ensures that the entire lighting system can obtain flat top light with different wavelength ranges, which is suitable for different types of light sources. Cells are sorted.

Further, FIG. 8 is a schematic structural diagram of an illumination system of another flow cytometer according to an embodiment of the present invention. As shown in FIG. 8 , the multi-wavelength light source unit 11 includes a plurality of single-mode fiber-coupled lasers 102 . The illumination system of the flow cytometer provided in the embodiment of the invention may further include a plurality of collimating lenses 50 and a plurality of beam turning devices 60. The collimating lenses 50 are in one-to-one correspondence with the single-mode fiber-coupled lasers 102, and the beam turning devices 60 are in one-to-one correspondence. One-to-one correspondence with the collimating lens 50; the collimating lens 50 is located on the propagation path of the outgoing laser light of the single-mode fiber-coupled laser 102, and is used for collimating the outgoing laser light to obtain a collimated laser beam; the beam turning device 60 is located in the collimating On the propagation path of the laser beam 50 , it is used to reflect the collimated laser beam to the geometric center of the cylindrical aspheric lens group 201 .

It can be understood that, the illumination system of the flow cytometry sorter provided by the embodiment of the present invention can be applied to the situation of multiple different laser light sources. Exemplarily, as shown in FIG. 8 , the plurality of single-mode fiber-coupled lasers 102 may be respectively 102a, 102b and 102c, the plurality of collimating lenses 50 may be respectively 50a, 50b and 50c, and the plurality of beam turning devices 60 may be respectively 60a, 60b and 60c. Wherein, the collimating lens 50a corresponds to the single-mode fiber-coupled laser 102a, and the beam turning device 60a corresponds to the collimating lens 50a; the collimating lens 50b corresponds to the single-mode fiber-coupled laser 102b, and the beam turning device 60b corresponds to the collimating lens 50b; The collimating lens 50c corresponds to the single-mode fiber-coupled laser 102c, and the beam turning device 60c corresponds to the collimating lens 50c.

Specifically, the collimating lenses 50a, 50b and 50c collimate the divergent laser beam emitted by the single-mode fiber-coupled laser 102 into a beam with a specific aperture with a smaller divergence angle. The collimating lens 50 may be a fixed-focus lens or a zoom lens, which is not limited in the embodiment of the present invention, and the emitted beam after being collimated by the collimating lens 50 is still a Gaussian beam, and the beam quality factor is less than 1.2.

Further, the beam deflecting devices 60a, 60b and 60c are used for integrating the collimated laser beams of different wavelengths, incident into the beam shaping unit 20 at a specific angle and position, and finally incident into the center of the flow chamber 40 . As shown in FIG. 8 , the beam turning devices 60a, 60b and 60c are used to integrate the collimated laser beams of different wavelengths into the same position in the beam shaping unit 20. Therefore, it is necessary to reasonably set the beam turning devices 60. The position and deflection angle ensure that laser beams of different wavelengths can be integrated together after passing through the beam deflecting device 60 .

Further, the core diameter d of the single-mode fiber-coupled laser 102 can satisfy 0<d<5 μm, and the laser wavelength λ emitted by the single-mode fiber-coupled laser 102 can satisfy 370nm≤λ≤700nm.

Exemplarily, the core diameter d of the single-mode fiber-coupled laser 102 is set to satisfy 0<d<5 μm, which ensures that the core diameter of the single-mode fiber-coupled laser 102 is small and the quality of the outgoing beam is good enough. Further, the laser wavelength λ emitted by the single-mode fiber-coupled laser 102 satisfies 370nm≤λ≤700nm, which ensures that the illumination system provided by the embodiment of the invention is suitable for the entire visible light range.

Further, FIG. 9 is a schematic diagram of a plurality of flat-top elliptical beams provided by an embodiment of the present invention. As shown in FIG. 9 , laser beams of different wavelengths are incident on the beam shaping unit 20 at the same position of the beam shaping unit 20, The beam shaping unit 20 is then separately incident to different positions of the flow chamber 40 . The light spots corresponding to the three laser beams may be arranged at equal intervals, and the distance d between any two adjacent light spots satisfies 120 μm≤d≤250 μm.

Further, when the light source unit 10 may include a multi-wavelength light source unit 11 corresponding to a plurality of laser beams of different wavelengths, the illumination system of the flow cytometer provided in the embodiment of the present invention may further include an achromatic lens to eliminate the differences due to different wavelengths. Chromatic aberration problems caused by wavelength laser beams. 1 , 5 , 6 , 7 and 8 , the illumination system of the flow cytometer provided by the embodiment of the present invention may further include an achromatic doublet lens 30 , and the achromatic doublet lens 30 is located in the On the propagation path of the transmitted light transmitted by the cylindrical aspheric lens group 201; the focal length f3 of the achromatic doublet lens 30 can satisfy 20mm<f2<100mm.

Exemplarily, in order to eliminate the problem of chromatic aberration caused by laser beams of different wavelengths, the illumination system of the flow cytometer provided in the embodiment of the present invention may further include an achromatic lens, and in the embodiment of the present invention, only the achromatic doublet lens 30 is used. As an example to illustrate, in this way, the chromatic aberration caused by laser beams of different wavelengths can be eliminated, and the structure of the achromatic lens can be simple. Further, the focal length f3 of the achromatic doublet lens 30 can satisfy 20mm<f2<100mm, and the focal length of the achromatic doublet lens 30 can be reasonably set to ensure that the achromatic doublet lens 30 can match the size of the incident light beam, so as to ensure the final flatness obtained. The size of the top spot meets the requirements.

Based on the same inventive concept, an embodiment of the present invention also provides a flow cytometry sorter, including the illumination system of the flow cytometry sorter described in the embodiment of the present invention, the flow cytometer sorter is the same as the above flow cytometry sorter. The lighting system of the type cell sorter has the same beneficial effect, which will not be repeated here.

Note that the above are only preferred embodiments of the present invention and applied technical principles. Those skilled in the art will appreciate that the present invention is not limited to the specific embodiments described herein, and that features of various embodiments of the present invention may be coupled or combined with each other in part or in whole, and may cooperate with each other and technically in various ways driven. Various obvious changes, readjustments, mutual combinations and substitutions can be made by those skilled in the art without departing from the protection scope of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and can also include more other equivalent embodiments without departing from the concept of the present invention. The scope is determined by the scope of the appended claims.

Claims (10)

1. An illumination system of a flow cytometry sorter, characterized in that, comprising a light source unit and a beam shaping unit, and the beam shaping unit is located on the propagation path of the outgoing light of the light source unit; The beam shaping unit includes a cylindrical aspheric lens group, and the cylindrical aspheric lens group includes a first conical surface and a second conical surface; The first conical surface has a first optical power

The second conical surface has a second optical power

in

2 . The lighting system according to claim 1 , wherein the radius of curvature of the first conical surface is R _y1 , the conic coefficient of the first conical surface is k _y1 , and |R _y1 |<100mm, 3 . -1000<k _y1 <-1; The radius of curvature of the second conical surface is R _y2 , the conic coefficient of the second conical surface is k _y2 , and |R _y2 |<100mm, -1000<k _y2 <-1. 3 . The lighting system according to claim 1 , wherein the focal length f1 of the first conical surface satisfies 0mm<f1<250mm, and the focal length f2 of the second conical surface satisfies -250mm<f2<0mm. 4 . 4. The lighting system according to any one of claims 1-3, wherein the cylindrical aspheric lens group comprises a first cylindrical aspheric lens and a second cylindrical aspheric lens, and the first cylindrical aspheric lens The cylindrical aspheric lens includes the first conical surface, and the second cylindrical aspheric lens includes the second conical surface; The first cylindrical aspheric lens is located on the propagation path of the outgoing light from the light source unit, and the second cylindrical aspheric lens is located on the propagation path of the transmitted light transmitted by the first cylindrical aspheric lens Or, the second cylindrical aspheric lens is located on the propagation path of the outgoing light of the light source unit, and the first cylindrical aspheric lens is located in the transmission obtained by the second cylindrical aspheric lens. on the propagation path of the light. 5 . The lighting system according to claim 1 , wherein the cylindrical aspheric lens group comprises a third cylindrical aspheric lens, and the third cylindrical aspheric lens comprises the a first conical surface and the second conical surface; The first conical surface is located on the propagation path of the outgoing light from the light source unit, and the second conical surface is located on the propagation path of the transmitted light obtained through the first conical surface; or, the second cone The surface is located on the propagation path of the outgoing light from the light source unit, and the first conical surface is located on the propagation path of the transmitted light transmitted through the first conical surface. 6 . The lighting system according to claim 1 , wherein the light emitted from the light source unit is passed through the beam shaping unit to obtain a flat-top elliptical beam; 6 . along the long axis direction of the flat-top elliptical beam, the light intensity of the flat-top elliptical beam is uniformly distributed; along the short-axis direction of the flat-top elliptical beam, the light intensity of the flat-top elliptical beam is Gaussian distribution; The spot size of the flat-top elliptical beam in the direction of the long axis is the first spot size, where the first spot size Wx is the beam diameter corresponding to the light intensity I _w1 , where I _w1 is the long axis 1/e ² times the maximum light intensity in the direction; The second spot size Wt is the beam diameter corresponding to when the light intensity uniformity satisfies a preset value, wherein the preset value a satisfies 95%≤a≤100%, and Wt/Wx>75%. 7. The lighting system according to claim 1, wherein the light source unit comprises a multi-wavelength light source unit. 8. The lighting system according to claim 7, wherein the multi-wavelength light source unit comprises a plurality of single-mode fiber-coupled lasers; The illumination system further includes a plurality of collimating lenses and a plurality of beam turning devices, the collimating lenses are in one-to-one correspondence with the single-mode fiber-coupled lasers, and the beam turning devices are in one-to-one correspondence with the collimating lenses; The collimating lens is located on the propagation path of the outgoing laser light of the single-mode fiber-coupled laser, and is used for collimating the outgoing laser light to obtain a collimated laser beam; The beam turning device is located on the propagation path of the collimated laser beam, and is used for reflecting the collimated laser beam to the geometric center of the cylindrical aspheric lens group. 9 . The lighting system according to claim 8 , wherein the lighting system further comprises an achromatic doublet lens, and the achromatic doublet lens is located in the transmitted light obtained by the cylindrical aspheric lens group. 10 . on the propagation path; The focal length f3 of the achromatic doublet lens satisfies 20mm<f2<100mm. 10 . A flow cytometry sorter, characterized in that it comprises an illumination system of the flow cytometry sorter according to any one of claims 1 to 9 . 11 .

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