CN112568228B - Insecticidal composition - Google Patents

Abstract

The invention relates to an insecticidal composition which contains an active ingredient compound shown in a formula I and a compound II, wherein the compound II is at least one selected from trifluoro-benzene pyrimidine, thiocyclam, antifeedant hydrazine and bromantraniliprole. The invention also relates to the use of the insecticidal composition for preventing or controlling insects of the families of the borer moth, plutellaceae, noctuid, leaf beetle, plant hopper, aphid and leaf beetle.

Description

An insecticide composition

Technical Field

The present invention relates to an insecticidal composition which is highly effective in controlling animal pests.

Background Art

The most typical problem in animal pest control is the need to reduce the dosage rate of the active ingredient to reduce or avoid adverse environmental or toxicological effects while still providing effective animal pest control.

Another difficulty with pest control is that the repeated and exclusive use of a single insecticidal active ingredient in many cases leads to the rapid selection of pests that develop natural or adapted resistance to the active ingredient.

Due to the multiple generations and adaptability of pests, pest resistance is developed in a relatively short period of time. There is also a need for pest control agents that act on pests and their larvae and eggs, and that have excellent permeability.

In addition, in pest control, it is also necessary to combine knockdown activity and extended control time, that is, rapid action and long-lasting action.

Active ingredients of the formula I are known from WO 2007/144100. However, the insecticidal/acaricidal efficacy of the known compounds and/or their spectrum of activity and/or their compatibility with plants, in particular with crop plants, is not always sufficient.

Summary of the invention

The object of the present invention is to provide an insecticidal composition that solves at least one of the problems such as improved biological properties, providing synergistic properties, reducing dosage rates, expanding the spectrum of activity, combining knockdown activity and long-lasting action, resistance management to delay resistance.

The present invention unexpectedly found that the combination of the compound of formula I and compound II, where compound II is selected from one of trifluanid, thiocarb, hydrazine, and bromofenac, can effectively prevent or control animal pests and their larvae and eggs. Surprisingly, it has been found that the simultaneous (i.e., combined or separate) application of the active compound compound of formula I and compound II or the sequential application of the compound of formula I and compound II results in an enhanced pest control effect compared to the possible control rate of each compound, and this unexpected effect is a true synergistic effect, rather than just the addition of activity.

An insecticide composition of the present invention is implemented by the following technical scheme:

A pesticidal composition comprises active ingredients of a compound of formula I and a compound II, wherein the compound II is selected from one of the following compounds: trifluanid, thiocarb, hydrazine, bromofenac.

The compound II is selected from trifluorobenzopyrimidine.

The compound II is selected from parathion.

The compound II is selected from the group consisting of food-inhibiting hydrazines.

The compound II is selected from bromofenac.

The weight ratio of the compound of formula I to compound II is 50:1-1:300, preferably 25:1-1:200, preferably 10:1-1:150, more preferably 10:1-1:100, more preferably 10:1-1:50.

The weight ratio of the compound of formula I to compound II of the present invention can also be, for example, 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:13, 1:15, 1:18, 1:20, 1:25, 1: 28. 1:30, 1:35, 1:40, 1:45, 1:50, 1:55, 1:60, 1:65, 1:70, 1:75, 1:80, 1:85, 1:90, 1:95, 1:100, 1:120, 1:150, 1:160, 1:180, 1:200, 1:220, 1:250, 1:280, 1:300.

A pesticidal composition comprising active compounds of formula I and compound II, wherein the weight of the compound of formula I and compound II together accounts for 1%-90% by weight of the pesticidal composition, preferably 5%-80%, more preferably 5%-70%, further preferably 5%-60%, and more preferably 5%-50%.

An insecticidal composition comprising active compounds of formula I and compound II, wherein the weight of the compound of formula I and compound II in total accounts for 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% of the weight of the insecticidal composition.

A pesticidal composition comprising active ingredients of a compound of formula I and a compound II, and further comprising a surfactant and/or a filler.

The insecticide composition of the present invention has the following dosage forms: emulsifiable concentrate, water suspension, oil suspension, seed treatment dry powder, seed treatment suspension, water dispersible granules, wettable powder, suspoemulsion, aerosol, coated granules, extruded granules, water emulsion, microcapsule suspension, microcapsule suspension-suspension, dry suspension, ultra-low volume liquid, electrostatic oil, gel, granule and microgranule.

The insecticidal composition of the present invention can be applied undiluted or diluted with water.

The insecticidal composition of the present invention unexpectedly exhibits extremely high activity in preventing or controlling pests and their larvae and eggs in treating plants, plant propagation materials, and soil in which plants grow.

The insecticidal composition of the present invention has excellent controlling efficacy on pests and their larvae and eggs at a low dosage.

In another aspect, the present invention also provides a use of an insecticidal composition for preventing or controlling pests and their larvae and eggs.

In particular, the present invention provides an insecticidal composition for preventing or controlling pests of the Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, and Tetranychus idae.

More particularly, the present invention provides an insecticidal composition for preventing or controlling tobacco aphid, cotton aphid, peach aphid, diamondback moth, beet armyworm, fall armyworm, cotton bollworm, striped stem borer, rice leaf roller, yellow flea beetle, great monkey leafworm, small monkey leafworm, two-spotted spider mite pests and their larvae and eggs.

The insecticide composition of the present invention is used for treating seeds to prevent the seeds from being attacked by pests.

The insecticide composition of the present invention can be applied to seeds, plants or fruits of plants or soil where plants grow or soil suitable for plant growth.

In another aspect, the present invention also provides a method for preventing or controlling pests and their larvae and eggs, wherein the pesticidal composition of the present invention is applied to target useful plants, target pests or their environment, or propagation materials of target useful plants. The plant propagation materials are seedlings, rhizomes, nursery seedlings, cuttings or seeds.

A method for preventing or controlling pests and their larvae and eggs, comprising applying the pesticidal composition of the present invention to seeds, target useful plants or soil where plants grow or soil suitable for plant growth.

A method for preventing or controlling pests and their larvae and eggs, comprising applying the pesticidal composition of the present invention to soil before, after or before or after seed germination and/or directly applying it to soil in contact with plant roots or soil suitable for plant growth.

A method for protecting seeds comprises contacting the seeds with an insecticidal effective amount of the insecticidal composition of the present invention before sowing and/or after germination.

The plants include plant stems and leaves, plant flowers, plant fruits and the like.

The seeds are selected from the group consisting of potato, sunflower, coffee, tobacco, canola, rapeseed, sugar beet, tomato, cucumber, bean, Brassica, onion, soybean, wheat, barley, rye, oat, sorghum, peanut, sugarcane, rice, pakchoi, cabbage, cowpea, carrot, cotton and corn seeds.

A method for preventing or controlling pests, comprising applying a compound of formula I and a compound II separately, sequentially or simultaneously.

The pesticidal composition of the present invention can be applied in any desired manner, such as seed coating, soil drench and/or direct furrow application and/or foliar spraying, and can be applied before germination, after germination or before and after germination.

The insecticide composition of the present invention contains active ingredients of formula I compound and compound II, and solves at least one problem such as improving biological properties, providing synergistic properties, reducing dosage rates, expanding activity spectrum, combining knockdown activity and long-term effect, and resistance management to delay resistance.

The combination of the compound of formula I and compound II can effectively prevent or control animal pests and their larvae and eggs. Compared with the possible control rate of each compound, the simultaneous (i.e., combined or separate) application of the active compound compound of formula I and compound II or the sequential application of the compound of formula I and compound II obtains an enhanced pest control effect, and this unexpected effect is a true synergistic effect, rather than just the addition of activity.

DETAILED DESCRIPTION

For the convenience of understanding the present invention, the present invention lists the following embodiments. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

The present invention has found that the combination of the compound of formula I and the compound II can effectively prevent or control pests and their larvae and eggs. Surprisingly, the activity of the pesticidal composition of the present invention in controlling harmful organisms is much higher than the sum of the performance of the individual active compounds. This unexpected effect is a true synergistic effect, not just the addition of activity.

As used herein, the term "synergistic effect" means that the insecticidal effect of the active compound combination or composition of the present invention is greater than the sum of the effects of the individual active compounds, or in other words, the effect is superadditive.

The invention provides an insecticidal composition, which contains an active compound of formula I and a compound II, wherein the compound II is selected from one of the following compounds: trifluanid, thiocarb, hydrazine, and bromofenac.

The insecticide composition of the present invention contains the compound of formula I and trifluanid.

The insecticidal composition of the present invention contains the compound of formula I and an insecticide ring.

The insecticide composition of the present invention contains the compound of formula I and hydrazine.

The insecticide composition of the present invention contains the compound of formula I and brofenac.

The weight ratio of the compound of formula I to compound II is 50:1-1:300, preferably 25:1-1:200, preferably 10:1-1:150, more preferably 10:1-1:100, more preferably 10:1-1:50.

The weight ratio of the compound of formula I to compound II of the present invention can also be, for example, 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:13, 1:15, 1:18, 1:20, 1:25, 1: 28. 1:30, 1:35, 1:40, 1:45, 1:50, 1:55, 1:60, 1:65, 1:70, 1:75, 1:80, 1:85, 1:90, 1:95, 1:100, 1:120, 1:150, 1:160, 1:180, 1:200, 1:220, 1:250, 1:280, 1:300.

The more preferred specific composition is as follows:

In the insecticidal composition, when the compound II is selected from trifluanidine, the ratio of the compound of formula I to compound II is preferably 15:1-1:15; more preferably 10:1-1:10; more preferably 5:1-1:5.

In the insecticidal composition, when the compound II is selected from insecticide ring, the ratio of the compound of formula I to compound II is preferably: 10:1-1:50; more preferably 5:1-1:40; more preferably 1:1-1:30, more preferably 1:1-1:25.

In the insecticidal composition, when the compound II is selected from hydrazine, the ratio of the compound of formula I to compound II is preferably 5:1-1:100; more preferably 1:1-1:50; more preferably 1:1-1:25, more preferably 1:1-1:10.

In the insecticidal composition, when the compound II is selected from bromofenac, the ratio of the compound of formula I to compound II is preferably 15:1-1:15; more preferably 10:1-1:10; more preferably 5:1-1:5.

The preparation of compound II and its action against pests are known and disclosed on the website http://www.alanwood.net/pesticides.

The total amount of active ingredients in the insecticide composition of the present invention can be selected according to specific factors to achieve the desired effect, such as the dosage form, the object to be applied, the application method, etc.

The present invention provides an insecticidal composition, which contains active compounds of formula I and compound II, wherein the weight of the compound of formula I and compound II together accounts for 1%-90% by weight of the composition, preferably 5%-80%, more preferably 5%-70%, further preferably 5%-60%, and more preferably 5%-50%.

A pesticidal composition comprising active ingredients of a compound of formula I and a compound II, wherein the weight of the compound of formula I and the compound II in total may be, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% by weight of the pesticidal composition.

The insecticide composition of the present invention may optionally contain auxiliary ingredients such as agronomically acceptable surfactants and/or fillers. Preferably, the auxiliary ingredient is one or a mixture of a dispersant, a wetting agent, an antifreeze agent, a thickener, a defoaming agent, a disintegrant, a binder, and an auxiliary carrier.

According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to an object (e.g., a plant, a crop, or a grass). Therefore, the filler is preferably inert and should at least be agronomically acceptable. The filler can be solid or liquid.

The inactive filler that can be used in the present invention can be either solid or liquid.

Solid fillers include, for example, plant powders (such as soybean powder, starch, grain powder, wood powder, bark powder, sawdust, walnut shell powder, bran, cellulose powder, coconut shells, corn cobs and tobacco stem particles, residues after extracting plant essences, etc.), clays (such as kaolin, bentonite, acidic porcelain clay, etc.), talc, silicas (such as diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (pumice, attapulgite and zeolite, etc.), and fired diatomaceous earth.

The liquid filler may be selected from the following materials, such as water, isopropanol, butanol, ethylene glycol, propylene glycol, ethyl acetate, methyl oleate, amides, lactams (such as N-methylpyrrolidone), dimethyl sulfoxide, mineral and vegetable oils, etc.

Surfactants can be used to emulsify, disperse, solubilize and/or wet the active ingredient compounds. For example, fatty alcohol polyoxyethylene ethers, polyoxyethylene alkyl aryl ethers, polyoxyethylene higher fatty acid esters, phosphate esters of polyoxyethylene alcohols or phenols, fatty acid esters of polyols, naphthalenesulfonic acid polymers, lignin sulfonates, high molecular comb-shaped branched copolymers, butylnaphthalenesulfonates, alkylarylsulfonates, sodium alkylsulfosuccinates, oils and fats, condensates of fatty alcohols and ethylene oxide, polyacrylates of alkyl taurates, protein hydrolysates, suitable oligosaccharides or polymers, for example based on vinyl monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly)alcohols or (poly)amines.

In order to disperse, stabilize and adhere the active ingredient compounds, auxiliary agents such as xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate and natural phospholipids (such as cephalin and lecithin) as well as synthetic phospholipids, bentonite, sodium lignin sulfonate, etc. can be used.

Among them, ethylene glycol, propylene glycol, glycerol, and sorbitol can be selected as antifreeze agents. As deflocculants for suspended products, auxiliary agents such as naphthalenesulfonic acid polymers and polyphosphates can be used. As defoamers, silicone defoamers can be used. To improve the fluidity of solid products, auxiliary agents such as paraffin, stearate, and alkyl phosphate can be used.

Colorants that can be used include, for example, inorganic pigments such as iron oxide, titanium oxide and Prussian blue; and organic pigments/dyes such as alizarin dyes, azo dyes and metal phthalocyanine dyes; and trace elements such as iron salts, manganese salts, boron salts, copper salts, cobalt salts, molybdenum salts and zinc salts.

Optionally, other additional components may also be included, such as protective colloids, adhesives, thickeners, thixotropic agents, penetrants, stabilizers, and masking agents.

The insecticidal composition of the present invention can be used by itself, or can be used in the form of its preparation or the use form prepared therefrom according to its respective physical and/or chemical properties, such as aerosol, microcapsule suspension, cold mist, hot mist, microcapsule granules, fine granules, suspension for seed treatment, ready-to-use solution, powder, emulsifiable concentrate, oil-in-water emulsion, water-in-oil emulsion, large granules, microgranules, oil-dispersible powder, oil suspension, oil, foam, paste, seed coating, suspension, suspension emulsion, aqueous solvent, suspension, wettable powder, soluble powder, dust and granules, water-soluble granules or tablets, water-soluble powder for seed treatment, wettable powder, natural products and synthetic substances impregnated with active ingredients, microcapsules in polymeric materials and seed coating materials, and ultra-low volume cold mist and hot mist preparations.

The preferred dosage forms of the insecticide composition of the present invention are emulsifiable concentrates, aqueous suspensions, oil suspensions, seed treatment dry powders, seed treatment suspensions, water dispersible granules, wettable powders, suspoemulsions, aerosols, coated granules, extruded granules, aqueous emulsions, microcapsule suspensions, microcapsule suspension-suspension concentrates, dry suspensions, ultra-low volume liquids, electrostatic oils, gels, granules, and microparticles.

The pesticidal composition of the present invention can also be used in combination with other active ingredients, such as fungicides, bactericides, attractants, insecticides, miticides, nematicides, growth regulators, herbicides, safeners, fertilizers or chemical semioms.

The preparations of the present invention can be prepared by mixing the active ingredients with conventional additives in a known manner, such as conventional extenders and solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, waterproofing agents, and, if necessary, driers and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

The insecticidal compositions of the present invention include not only those that can be immediately applied to the object to be treated by means of suitable equipment such as spraying or dusting equipment, but also concentrated commercial compositions that need to be diluted before application to the object.

The insecticidal composition of the present invention may be formulated into a preparation suitable for application in the form of a combination of its active ingredient compounds, or may be applied in the form of a combination of its commercial preparations at appropriate dosages.

The insecticidal composition of the present invention has good plant compatibility and favorable homeothermic animal toxicity, and is suitable for controlling harmful organisms encountered in agriculture, forestry, protection of stored products and materials, and health fields, especially Insecta, Arachnida, Nematoda. They are active against usually sensitive and resistant species, and are active against all or individual development stages, and also achieve a surprising "synergistic" effect of preventing or controlling harmful organisms.

The present invention also provides a use of the insecticidal composition of the present invention for preventing or controlling harmful organisms. The term "harmful organism" used herein means any organism that can cause damage to the normal state of a plant, including harmful insects, arachnids, and nematodes.

The class Insecta includes the orders Lepidoptera, Coleoptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Thysanoptera, Isopoda, Diplopoda, Chilopoda, Synplasia, Thysanura, Collembola, Collembola, Orthoptera, Blattodea, Dermoptera, Isoptera, and Phycoptera.

Lepidoptera pests: Pyralidae, such as Chilo suppressalis (walker), Tryporyza incertulas (walker), Cnaphalocrocismedinalis Guenee, Hellullaundalis, Conogethes punctiferlis; Pieridae, such as Pieris rapae rapae), papilioxuthus, pierisrapaecrucivora, parnaraguttata; Arctiidae such as the white moth (hyphantriacunea); Noctuidae such as the cabbage looper (trichoplusiani), cabbage looper (mamestrabrassicae), beet armyworm (spodopteraexigua), cutworm (spodopteralitura), cotton bollworm (helicoverpaarmigera), oriental armyworm (Pseudaletia separate), cutworm (Agrotis ipsilon); Tortricidae such as the tea leaf roller (adoxophyesoranafasciata), apricot leaf roller (archipsfuscocureanus), tea leaf roller (homonamagnanima); Plutellidae Such as the diamondback moth (Plutella xylotella); Gelechiidae such as the pink bollworm (Pectinophoragossypiella) and the pink bollworm (Pink bollworm);

Coleoptera pests, such as: rice weevil (sitophilus oryzaeline), citrus leaf miner (PodagricomelanigricollisChe), corn weevil (S.zeamails), grain weevil (S.granarius), cabbage leaf beetle (Cabbageleafbeetle), small monkey leaf beetle (Daikon leaf beele), flea beetle (fleabeetle), grape flea beetle (Alticachalybea), curved flea beetle (phyllotretastriolata), cucumber flea beetle (Epitrixcucumeris), tobacco flea beetle (Ehirtipennis), eggplant flea beetle (E.fuscula), yellow melon beetle (Aulacophoraindica (Gemlin), mustard leaf beetle (Phaedoncochleariae), rice water weevil (Lissorhoptrus oryzophilus), Callosobruchuyschienensis, yellow mealworm (Tenebrio molitor), corn rootworm (Diabrotica virgiferavirgifera), cucumber beetle (Diabrotica undecimpunctata howardi), copper beetle (Anomalacuprea), red copper beetle (Anomala rufocuprea), yellow flea beetle (Phyllotreta striolata), yellow melon beetle (Aulacophora femoralis), potato beetle (Leptinotarsa decemlineata), rice mudworm (Oulema oryzae), bostrychidae and Cerambycidae;

From the order of the Diptera, for example, Aedes spp., Anopheles spp., Bibio hortulanus, Calliphora erythrocephala, Ceratitis capitata, Chrysomyia spp., Cochliomyia spp., Cordylobia anthropophaga, Culex spp., Cuterebra spp., Dacus oleae, Dermatobia hominis, Drosophila spp., Fannia spp., Gastrophilus spp., Hylemyia spp., Hypoderma spp., Liriomyza spp.), Lucilia spp., Musca spp., Nezara spp., Oestrus spp., Oscinella frit, Pegomyia hyoscyami, Phorbia spp., Stomoxys spp., Tabanus spp., Tannia spp., Tipula paludosa, Wohlfahrtiaspp.

From the order of the Heteroptera, for example, Anasa tristis, Antestiopsis spp., Blissus spp., Calocoris spp., Campylomma livida, Cavelerius spp., Cimex spp., Creontiades dilutus, Dasynus piperis, Dichelops furcatus, Diconocorishewetti, Dysdercus spp., Euschistus spp., Eurygaster spp., Heliopeltis spp., Horcias nobilellus, Leptocorisa spp., Leptoglossus phyllopus, Lygus spp., Macropes excavatus, Miridae, Nezara Amyot et Serville, Piesma quadrata, Piezodorus spp., Psallus seriatus, Pseudacystapersea, Rhodnius spp., Sahlbergella singularis, Scotinophora spp., Stephanitis nashi, Tibraca spp., Triatoma spp.

From the order of the Homoptera, for example, Acyrthosipon spp., Aeneolamiaspp., Agonascena spp., Aleurolobus barodensis, Aleurothrixus spp., Amrasca spp., Anuraphis cardui, Aonidiella spp., Aphanostigma piri, Aphis spp., Arboridia apicalis, Aspidiella spp., Aspidiotus spp., Atanus spp., Aulacorthum solani, Bemisia spp., Brachycaudus helichrysii), Brachycolus spp., Brevicoryne brassicae, Calligypona marginata, Carneocephala fulgida, Ceratovacuna lanigera, Cercopidae, Ceroplastes spp., Chaetosiphon fragaefolii, Chionaspis tegalensis, Chlorita onukii, Chromaphis juglandicola, Chrysomphalus ficus, Cicadulina mbila, Coccomytilus halli, Coccus spp., Cryptomyzus ribis, Dalbulus spp., Dialeurodes spp., Diaphorina spp., Diaaspis spp., Doralis spp., Drosichas spp., Dysaphis spp., Dysmicoccus spp., Empoasca spp., Eriosoma spp., Erythroneura spp., Euscelis bilobatus, Geococcus coffeae, Homalodis cacoagulata, Hyalopterus arundinis, Icerya spp., Idiocerus spp., Idioscopus spp.), Laodelphax striatellus, Lecanium spp., Lepidosaphes spp., Lipaphis erysimi, Macrosiphum spp., Mahanarva fimbriolata, Melanaphis sacchari, Metcalfiella spp., Metopolophium dirhodum, Monellia costalis, Myzus spp., Nasonovia ribisnigri, Nephotettix spp., Parabemisia myricae, Parlatoria spp., Pemphigus spp., Peregrinus maidis), Phenacoccus spp., Phloeomyzus passerinii, Phorodon humuli, Phylloxera spp., Pinnaspis aspidistrae, Planococcus spp., Protopulvinaria pyriformis, Pseudaulacaspis pentagona, Pseudococcus spp., Psylla spp., Pteromalus spp., Pyrillaspp., Quadraspidiotus spp., Quesada gigas, Rastrococcus spp., Rhopalosiphum spp., Saissetia spp., Scaphoides titanus, Schizaphis graminum, Selenaspidusarticulatus, Sogata spp., Sogatella furcifera, Nilaparvata lugens, Tomaspis spp., Toxoptera spp., Trialeurodes vaporariorum, Trioza spp., Typhlocyba spp., Unaspis spp., and Viteus vitifolii.

From the order of the Hymenoptera, for example, Diprion spp., Hoplocampas spp., Lasius spp., Monomorium pharaonis, Vespa spp.

From the order of the Orthoptera, for example, Acheta domesticus, Blattaorientalis, Blattella germanica, Gryllotalpa spp., Leucophaea maderae, Locusta spp., Melanoplus spp., Periplaneta americana, Schistocerca gregaria, Acrididae (Acrididae); megacopta punctatissimum, eurydemarugosum, eysarcoris lewisi, eysarcoris parvus, nezara viridula, plautiastali, halymorphamista, cletus punctiger, leptocorisa chinensis, stephantis pyrioides, trigonotylus coelestialium.

From the order of the Thysanoptera, for example, Baliothrips biformis, Enneothrips flamens, Frankliniella spp., Heliothrips spp., Hercinothrips femoralis, Kakothrips spp., Rhipiphorothrips cruentatus, Scirtothrips spp., Taeniothrips cardamoni, Thrips spp.

From the order of the Isoptera, for example, Reticulitermes spp., Odontotermes spp.

From the order of the Thysanura, for example, Lepisma saccharina.

From the order of the Anoplura, Phthiraptera, for example, Damalinia spp., Haematopinus spp., Linognathus spp., Pediculus spp., Trichodectes spp.

Pests of the order Acarina, class Arachnida. Examples include Panonychus citri, Tetranychus cinnabarinus, Panonychus ulmi, Tetranychus surticae, Tetranychus viennensis, Oligonychus ununguis, Eotetranychus kankitus, Brevipalpus phoenicis, and Bryobi apraetiosa), wheat leaf curler (Aceriatulipae), grape gall mite (Colacarusvitis), tea leaf gall mite (Calacaruscarinatus), tea yellow mite (Polyphagotarsonemuslatus), long-haired root mite (Rhizoglyphusrostochiensis), rough-legged flour mite (Acarussiro), citrus gall mite (Aceriasheldoni), Schlechtendali needle gall mite (Aculusschlechtendali), Tetranychus spp., Panonychus spp., Phyllocoptrutaspp., Brevipalpus spp., etc.

Class Bivalva, for example, Dreissena spp.

From the order of the Chilopoda, for example, Geophilus spp., Scutigera spp.

From the order of the Collembola, for example, Onychiurus armatus.

From the order of the Dermaptera, for example, Forficula auricularia.

From the order of the Diplopoda, for example, Blaniulus guttulatus.

From the class of the Gastropoda, for example, Arion spp., Biomphalaria spp., Bulinus spp., Deroceras spp., Galba spp., Lymnaea spp., Oncomelania spp., Succinea spp.

From the order of the Isopoda, for example, Armadillidium vulgare, Oniscusasellus, Porcellio scaber.

From the order of the Siphonaptera, for example, Ceratophyllus spp., Xenopsylla cheopis.

From the order of the Symphyla, for example, Scutigerella immaculata.

Representative organisms of the class Nematoda are selected from the genera Meloidogyne spp., Heterodera spp., Globodera spp., Radopholus spp., Pratylenchus spp., Longidorus spp., etc. Preferred nematodes are the soybean cyst nematode Heterodera, golbodera spp., and meloidogyne incognita.

Particularly, the insecticidal composition of the present invention shows a surprising "synergistic" effect on preventing or controlling pests of Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus mites and their larvae and eggs.

Pyralidae, such as Chilo suppressalis (walker), Tryporyza incertulas (walker), Cnaphalocrocism medinalis Guenee, Hellullaundalis, Conogethes punctiferlis;

Noctuidae, such as the cabbage looper (trichoplusiani), cabbage armyworm (mamestrabrassicae), beet armyworm (spodopteraexigua), cutworm (spodopteralitura), cotton bollworm (helicoverpaarmigera), oriental armyworm (Pseudaletia separate), and cutworm (Agrotisipsilon);

Plutellidae, such as the diamondback moth (Plutellax ylotella);

Aphididae: such as Myzus persicae, Aphis gossypii, Brevicorynebrassicae, Cryptomyzus ribis, Aphis fabae, Aphispomi, Eriosoma lanigerum, Hyalopterus arundinis, Phylloxera vastatrix, Macrosiphum avenae, Metopolophium dirhodum, Rhopalosiphum padi, Schizaphis graminum;

Delphacidae: Gray planthopper (Laodelphax striatellus), brown planthopper (Nilaparvata lugensstal), white-backed planthopper (Sogatella furcifera), sugarcane flat-horned planthopper (Perkinsiella saccharicida Kirkaldy);

Chrysomelidae: Phyllotreta striolata, Aulacophorafemoralis, Cabbageleafbeetle, Daikon leaf beele;

Tetranychus citri, Tetranychus cinnabarinus, Panonychus ulmi, Tetranychus urticae, Tetranychus viennensis, Eotetranychus kankitus, Aceriatulipae.

More particularly, the insecticide combination of the present invention is particularly effective for preventing or controlling pests such as tobacco aphid, cotton aphid, peach aphid, diamondback moth, beet armyworm, fall armyworm, cotton bollworm, striped stem borer, rice leaf roller, yellow flea beetle, great monkey leafworm, small monkey leafworm, two-spotted spider mite, citrus mites, cinnabar spider mite and their larvae and eggs.

The insecticidal composition of the present invention unexpectedly exhibits extremely high activity in preventing or controlling pests of Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus dae and their larvae and eggs in treating plants, plant propagation materials and soil where plants grow.

The insecticidal composition of the present invention has excellent control efficacy on pests of Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus dae and larvae and eggs thereof at a low dosage.

On the other hand, the present invention also provides a use of an insecticidal composition for preventing or controlling pests of the Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus dae and their larvae and eggs.

The invention provides an insecticide composition for preventing or controlling tobacco aphid, cotton aphid, peach aphid, diamondback moth, beet armyworm, fall armyworm, cotton bollworm, striped stem borer, rice leaf roller, yellow striped flea beetle, great monkey leafworm, small monkey leafworm, two-spotted spider mite pests and their larvae and eggs.

The pesticidal compositions of the present invention can be applied to any and all developmental stages of pests, such as eggs, larvae, pupae and adults. Pests can be controlled by contacting the target pests, their food supply, habitat, breeding ground or their location with an insecticidally effective amount of the pesticidal compositions of the present invention.

“Location” means plants, plant propagation material, soil, area, material or environment in which a pest is growing or may grow.

"Insecticidal effective amount" refers to the amount of the insecticidal composition of the present invention required to obtain an observable effect on growth, including a mortality, prevention or removal effect, a destruction effect, or an effect of reducing the appearance and activity of animal pests. The insecticidal effective amount of the various insecticidal compositions used in the present invention can vary. The insecticidally effective amount of the composition will also vary depending on the prevailing conditions such as the desired insecticidal effect and duration, weather, target species, location, mode of application, etc.

According to the present invention, all plants and parts of plants can be processed. "Plant" should be understood as representing all plants and plant populations such as desired and undesirable wild plants or crops (comprising naturally grown crops) at this. Crop can be the plant obtained by conventional breeding and optimization method, or the plant obtained by biotechnology and genetic engineering method or the combination of these methods, including transgenic plants and including plant cultivars that can or cannot be protected by plant breeder's certificate. Plant parts should be understood as representing all above-ground and underground parts and plant organs such as seedlings, leaves, flowers and roots, and the example that can be mentioned is leaf, needle, stem, dry, flower, fruit body, fruit and seed and root, tuber and rhizome. The part of plant also includes plant and asexual propagation and sexual propagation material after harvest, for example seedling, tuber, rhizome, cutting and seed.

As mentioned above, all plants and their parts can be treated according to the invention. In a preferred embodiment, wild plant species and plant cultivars, or those obtained by conventional biological breeding methods such as hybridization or protoplast fusion, and their parts are treated. In a more preferred embodiment, transgenic plants and plant cultivars and their parts obtained by genetic engineering (if necessary in combination with conventional methods (Genetically Modified Organisms)) are treated.

The insecticidal composition of the present invention has good plant compatibility and is suitable for controlling pests, especially insects, mites, nematodes, as well as fungi, rodents, microorganisms, etc., especially Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus, etc. It is preferably suitable for foliar treatment, soil treatment and seed treatment.

On the other hand, the present invention also provides a method for preventing or controlling pests and their larvae and eggs, wherein the pesticidal composition of the present invention is applied to target useful plants, target pests or their environment, or propagation materials of target useful plants. The method can be performed before or after the plants, plant propagation materials or environment are infested by pests.

The term "plant propagation material" should be construed as referring to all plant parts with reproductive capacity, for example seed, which can be used to breed the latter, and vegetable materials such as cuttings or tubers (for example potato). Therefore, plant parts used herein include plant propagation materials. For example, seed, root, fruit, tuber, bulb, rhizome and plant parts can be mentioned. Sprouting plants and effective plants that are suppressed after germination or emergence from soil. Young plants can be protected by all or local treatments before transplanting by dipping.

The plant propagation material is seedlings, rhizomes, nursery seedlings, cuttings or seeds. Seeds are preferred.

The insecticide composition of the present invention can be applied to seeds, plants or fruits of plants or soil where plants grow or soil suitable for plant growth.

A method for protecting seeds comprises contacting the seeds with an insecticidal effective amount of the insecticidal composition of the present invention before sowing and/or after germination.

The seeds are selected from the group consisting of potatoes, sunflowers, coffee, tobacco, rapeseed, rape, sugar beets, tomatoes, cucumbers, beans, Brassica plants, onions, soybeans, wheat, barley, rye, oats, sorghum, peanuts, sugar cane, rice, pakchoi, cabbage, cowpea, carrots, cotton and corn seeds. It is particularly important to process the seeds of wheat, barley, rye, oats, corn, cotton, rapeseed, rape and rice.

Thus, the present invention provides a method for protecting seeds, seedling roots and shoots from soil and foliar insect infestation, comprising contacting the seeds with an effective amount of the insecticidal composition of the present invention before sowing and/or after germination.

The present invention also provides a method for controlling or preventing pests and their larvae and eggs in plant propagation materials and in plant organs, plant parts and/or plants grown thereafter, comprising applying the pesticidal composition of the present invention to the plant propagation materials or their environment. The plant propagation materials are preferably seeds.

Most of the damage to crop plants caused by pests occurs as early as when the seed is infested, during storage and after the seed is sown in the soil and during or after germination of the plant. This phase is particularly critical, since the roots and shoots of the growing plant are particularly sensitive and even minor damage can lead to the death of the entire plant. Therefore, it is of particular interest to protect the seed and the germinating plant by using suitable compositions.

The present invention also particularly relates to a method for protecting seeds and germinating plants from pests and their larvae and eggs by treating the seeds with the pesticidal composition of the present invention. The method of the present invention for protecting seeds and germinating plants from Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus, and their larvae and eggs comprises a method for treating the seeds with a compound of formula I and a compound II at the same time. It also includes a method for treating the seeds with a compound of formula I and a compound II at different times.

A method for preventing or controlling pests and their larvae and eggs, comprising treating seeds for expected plant growth with a synergistically effective amount of the pesticidal composition of the present invention before sowing and/or after germination.

The invention also relates to the use of the insecticidal composition according to the invention for treating seeds to protect the seeds and the plants grown therefrom from attack by pests and their larvae and eggs.

Furthermore, the present invention also relates to seeds treated with the pesticidal composition of the present invention to thereby obtain protection from pests and their larvae and eggs.

The present invention also relates to seeds treated with the compound of formula I and compound II simultaneously. The present invention also relates to seeds treated with the compound of formula I and compound II at different times. For seeds treated with the compound of formula I and compound II at different times, the individual active compounds of the pesticidal composition of the present invention may be present in different layers on the seeds. Among them, the layers containing the compound of formula I and compound II may optionally be separated by an intermediate layer. The present invention also relates to seeds in which the compound of formula I and compound II are applied as a coating component or as one or more additional layers outside the coating.

Insecticidal composition of the present invention can be applied to the seed of any physiological state.Preferably seed is in a sufficiently durable state, thereby is not damaged during the treatment process.Generally, seed can be harvested from the field, removed from the plant, separated from the seed of any cob, stem, husk and surrounding pulp or other non-seed plant material.Seed can also preferably be biologically stable, to reach the degree that treatment does not cause biological damage to seed.Can be applied to seed at any time between seed harvesting and seed sowing or during the sowing process.Seed can also be germinated before or after treatment.

The seeds treated with the pesticidal composition of the present invention can be stored, managed, sown and cultivated.

Seed treatment can be achieved by applying thereto a compound of the formula I and a compound II in any desired order or simultaneously.

Seed treatment occurs on unsown seeds, the term "unsown seeds" being intended to include seeds at any stage between seed harvest and seed sowing in the ground for plant germination and growth purposes. Preferably, treatment occurs before seed sowing, so that the sown seeds have been pretreated with the combination. In particular, seed coating or seed pelleting is preferred in the treatment of the inventive combination. After treatment, the ingredients in each combination adhere to the seeds and can therefore be used for pest control.

Seed treatment methods include all methods known to the person skilled in the art that are suitable for treating seeds, such as seed dressing, seed coating, seed soaking, seed coating, seed multilayer coating, seed shelling, seed soaking, seed dusting and seed pelleting.

The seeds treated with the insecticidal composition of the present invention provide protection from pests not only for the seeds themselves but also for the plants grown from the seeds after their emergence. Therefore, it is not necessary to directly treat the plants at the time of sowing or soon thereafter.

The composition of the present invention can be applied to seeds, plants or fruits of plants or soil where plants grow or soil suitable for plant growth.

The present invention also provides a method for preventing or controlling pests and their larvae and eggs, comprising applying the pesticidal composition of the present invention to seeds, target useful plants or soil where plants grow or soil suitable for plant growth.

On the other hand, the present invention also provides a method for preventing or controlling pests and their larvae and eggs, wherein the insecticidal composition of the present invention is applied to the soil before, after or before or after seed germination and/or directly applied to the soil in contact with plant roots or soil suitable for plant growth.

The present invention also provides a method for protecting plants from pests and their larvae and eggs, comprising applying the pesticidal composition of the present invention to an environment, habitat or storage area where useful plants grow. The environment and habitat where plants grow refer to a support that enables crops to take root and grow, such as soil, water, etc. Specific raw materials can be sand, pumice, vermiculite, diatomaceous earth, agar, gelatinous substances, polymer substances, asbestos, sawdust, bark, etc. Soil is preferred.

The present invention also provides a method for protecting plants from attack by Pyralidae, Plutella xylostellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus and their larvae and eggs, comprising applying the active components compound of formula I and compound II in combination, separately or sequentially. In the case of separate application, the order generally has no effect on the results of the control measures.

A method for preventing or controlling pests and their larvae and eggs, comprising applying a compound of formula I and a compound II separately, sequentially or simultaneously.

The invention also provides a method for protecting useful plants from attack by pests and their larvae and eggs, which comprises applying a combination comprising a compound of formula I and a compound II to the target useful plants or their environment, to the target pests or their environment, to the propagation material of the target useful plants in any desired order or simultaneously.

The insecticidal compositions according to the invention include both ready-to-use compositions which can be applied to plants or plant propagation materials using suitable devices and commercial concentrates which have to be diluted with water before application.

The insecticidal composition of the present invention can be applied undiluted or diluted with water.

The present invention provides a method for preventing or controlling pests and their larvae and eggs, wherein the pesticidal composition of the present invention is applied to target useful plants or their environment, or propagation materials of target useful plants, and other pesticides can be applied before applying the compound of formula I and compound II, simultaneously with the compound of formula I and compound II, or independently of each other after applying the compound of formula I and compound II. In the case where the compound of formula I and compound II are applied separately, each other pesticide can be applied before applying the compound of formula I and compound II, simultaneously with the compound of formula I and compound II, between the compound of formula I and compound II, or independently of each other after applying the compound of formula I and compound II.

Methods for treating target pests, target useful plants, or seeds, soil with the insecticidal composition of the present invention include, for example, spreading treatment, soil treatment, surface treatment and fumigation treatment. Spreading treatment includes, for example, spreading, spraying, atomizing, and granule application. Soil treatment includes, for example, soil irrigation and soil mixing. Surface treatment includes, for example, coating and covering. Fumigation treatment includes, for example, covering the soil with a polyethylene film after injection into the soil. The preferred direct treatment of plants is foliar application treatment.

Applicators usually use the insecticidal composition of the present invention in a small backpack sprayer, spray tank, spray plane or irrigation system. The insecticidal composition of the present invention is usually formulated with water, buffer and/or other adjuvants to the desired application concentration to obtain a ready-to-use spray solution.

Compared with the prior art, the present invention has at least the following beneficial effects:

(1) The insecticidal composition of the present invention can show synergistic activity compared to the activity of a single compound;

(2) The plants and plant propagation materials treated with the insecticidal composition of the present invention reach a level showing significant improvement;

(3) The insecticidal composition of the present invention reduces the infestation and attack of any undesirable pests and their larvae and eggs; in particular, the Pyralidae, Plutellae, Noctuidae, Chrysomelidae, Delphacidae, Aphididae, Tetranychus, and their larvae and eggs;

(4) The present invention mixes the compound of formula I and compound II to solve the problem of sustained effect and rapid effect, thereby reducing the number of medications and reducing labor costs;

(5) The insecticide composition of the present invention exhibits high efficiency, thereby reducing the dosage rate and the load on the environment;

Biological test cases

The expected effect of a specific combination of two active ingredients can be calculated using the so-called "Colby formula" (see S.R.Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22):

X is the control effect when the active ingredient compound of formula I is used at a dosage of mg/ha or a concentration of m ppm, expressed as a percentage of the untreated control,

Y is the control effect when the active ingredient compound II is used at a dosage of ng/ha or a concentration of n ppm, expressed as a percentage of the untreated control,

E is the control effect when the active components of the compound of formula I and compound II are used in an amount of m and n g/ha or a concentration of m and n ppm, expressed as a percentage of the untreated control,

If the actual control effect exceeds the calculated value, the lethal effect of the combination is superadditive, that is, there is a synergistic effect.

Test Example 1: Cabbage aphid test

Preparation of drug solution: The original drug of the compound of formula I (synthesized by the method described in WO2007/144100) and the compound II in Table 1 were dissolved in acetone to prepare a single dose mother solution, and then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

The peach aphid (Myzus persicae Sulzer) was used as the test pest. It was collected from the cabbage vegetable field in Kunshan, Jiangsu Province. After one generation of reproduction on insect-free cabbage planted indoors under insect-proof nets, 30 adult peach aphids with similar body color and uniform size were selected as a group for indoor experiments.

Soak aphids and cabbage leaves in the prepared solution for 10 seconds, take them out, use absorbent paper to absorb excess solution, and put them in a plastic beaker with a diameter of 6cm and a height of 10cm. After the test, transfer them to a constant temperature incubator at 25±2 ^o C and a relative humidity of 60%-80% for breeding. The control treatment is a blank control with a treatment without the drug. Check the test results 72 hours after the drug treatment. If the control mortality rate is greater than 20%, repeat the test.

Calculation method:

Mortality rate (%) = (number of live worms before medication - number of live worms after medication) / number of live worms before medication × 100

Control effect % = ((mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)) × 100

Table 1 Test of the combination of compound I and compound II against peach aphid

Experiment 2 Diamondback moth experiment

Preparation of drug solution: The compound of formula I (synthesized by the method described in WO2007/144100) and the original drug of compound II in Table 2 were dissolved in acetone to prepare a single-dose mother solution, which was then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

Diamondback moth (Plutella xylostella Linnaeus). Indoor breeding population at Dongfangcun Farm, Kunshan, Jiangsu. Indoor experiment was conducted with 10 moths per group.

Immerse the cabbage leaves in the solution for 10 seconds, dry them, and place them in a glass culture dish (60 cm in diameter) lined with filter paper. Keep the petioles moist with wet cotton balls. Insert the third-instar larvae into the leaves, seal them with plastic wrap, cover them, and place them in a constant temperature incubator at 25±1 ^o C, relative humidity 60%-80%, and light cycle L:D=(16:8)h for observation.

The control treatment is a blank control without the drug. The results are checked after 72 hours in the incubator. The death judgment standard is: touch the insect body with a needle, and the insect body has no reaction or cannot react normally.

Calculation method:

Mortality rate (%) = (number of live worms before medication - number of live worms after medication) / number of live worms before medication × 100

Control effect % = ((mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)) × 100

Table 2 Control effect of the combination of compound I and compound II on Plutella xylostella

Test Example 3: Chilo suppressalis test

Preparation of drug solution: The compound of formula I (synthesized by the method described in WO2007/144100) and the original drug of compound II in Table 3 were dissolved in acetone to prepare a single-dose mother solution, and then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

Chilo suppressalis. Chilo suppressalis eggs were collected from Dongfangcun Farm in Kunshan, Jiangsu Province. After 2-3 generations of indoor rearing, the 2nd-mid-instar larvae were selected for testing.

Take a well-growing rice seedling and dry the surface water with absorbent paper. Soak in the prepared solution for about 10 seconds and dry naturally in the shade. Transfer to a test tube. Add 10 2nd instar larvae of Chilo suppressalis to each tube and plug it with cotton. Place it in a constant temperature incubator at 25±1 ^o C, relative humidity 60%-80%, and light cycle L:D=(16:8)h for observation. The control treatment is a blank control without the drug. Check the test results after 4 days. Insects that cannot crawl normally are considered dead.

If the control mortality rate is greater than 20%, the experiment should be repeated.

Calculation method:

Mortality rate (%) = (number of live worms before medication - number of live worms after medication) / number of live worms before medication × 100

Control effect % = ((mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)) × 100

Table 3 The control effect of the combination of the compound of formula I and compound II on Chilo suppressalis

Test Example 4: Rice leaf folder test

Preparation of drug solution: The original drug of the compound of formula I (synthesized by the method described in WO2007/144100) and compound II in Table 4 was dissolved in acetone to prepare a single dose mother solution, which was then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

The larvae of rice leaf folder (Cnaphalocrocis medinalis) were collected from Dongfangcun Farm in Kunshan, Jiangsu Province, and the third-instar test insects were raised indoors on rice seedlings for three generations.

Select fresh rice seedlings and immerse them in the test solution for 10 seconds. Take them out and put them in the prepared glass test tubes, dry them naturally, then put 10 3rd instar larvae of rice leaf folder into the test tubes, tie the tube mouth with gauze, and place them in the observation room for cultivation after the treatment.

The control treatment is a blank control without the drug. After 72 hours of treatment, the rice stems are peeled to check the death of the test insects and record them. The criterion for judging the death of the test insects is: no reaction when the test insects are gently touched with tweezers is considered dead. If the control mortality rate is greater than 20%, the test is repeated.

The culture conditions of the test targets and the targets after the test were a constant temperature incubator at 25±1 ^o C, relative humidity 60%-80%, and a light cycle of L:D=(16:8)h.

Calculation method:

Mortality rate (%) = (number of live worms before medication - number of live worms after medication) / number of live worms before medication × 100

Control effect % = ((mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)) × 100

Table 4 Control effect of the combination of compound I and compound II on rice leaf folder

Test Example 5: Rice brown planthopper test

Preparation of drug solution: The original drug of the compound of formula I (synthesized by the method described in WO2007/144100) and compound II in Table 5 was dissolved in acetone to prepare a single dose mother solution, which was then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

Brown planthopper (Nilaparvata lugens stal) nymphs were collected from the rice experimental field of Dongfang Village Farm in Kunshan, Jiangsu Province, and raised indoors with rice seedlings.

The temperature of the insect room is 28±2 ^o C, the relative humidity is above 70%, and the light is 14 hours per day. After the rice seeds are germinated, they are sown in a 20cm×30cm plastic box. When the rice seedlings grow to more than 10cm, the rice planthopper nymphs are raised to adults. The potted rice plants without rice planthopper eggs are placed in a 30cm×30cm×50cm insect cage. The female adults with eggs lay eggs for 2 to 3 days, and the rice seedlings are taken out and replaced with rice plants without eggs in the insect cage. After the brown planthopper eggs on the rice plants taken out of the insect cage are hatched, 3-day-old brown planthopper nymphs are taken for testing each time.

Select rice seedlings grown in the greenhouse, dig them up with roots, wash them, cut them into 10cm long rice stems with a few roots, and dry them in a cool place until there is no water mark on the surface. Soak the prepared rice stems in the liquid medicine for 30 seconds, take them out, dry them slightly, and place them in a 3cm×20cm test tube, 3 stems per tube. Seal the test tube with a black cloth and cover it with a wet towel to keep it moist.

Use an insect sucker to move the test insects into the test tube, 15 per test tube, and cover the tube mouth with gauze. The treated test insects are kept and observed under the conditions of 25±1 ^o C, relative humidity of 60%-80%, and light cycle of L:D=(16:8)h. Check the death of the test insects 48 hours after treatment and record it. The criterion for judging the death of the test insects is: no reaction when the test insects are gently touched with tweezers is considered dead. If the control mortality rate is greater than 20%, the test is repeated. The control treatment is a blank control with a treatment without the drug.

Calculation method:

Mortality rate (%) = (number of live worms before medication - number of live worms after medication) / number of live worms before medication × 100

Control effect % = ((mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)) × 100

Table 5 Control effect of the combination of compound I and compound II on rice brown planthopper

Test Example 6: Test on the yellow striped flea beetle

Preparation of drug solution: The original drug of the compound of formula I (synthesized by the method described in WO2007/144100) and the compound II in Table 6 were dissolved in acetone to prepare a single dose mother solution, and then diluted with an aqueous solution containing 0.1% Tween-80 to the required concentration.

The yellow striped flea beetle (Phyllotreta striolata) was used as the test pest and was collected from a vegetable field in Dongfang Village Farm, Kunshan, Jiangsu. Before the test, the yellow striped flea beetle was inoculated on the greenhouse potted cabbage planted indoors under the insect-proof net of the laboratory. After continuous feeding for 72 hours, the yellow striped flea beetle adults of the same size were selected for testing. The indoor test was carried out in groups of 20.

Dip the uncontaminated cabbage leaves in the test solution for 15 seconds, take them out, dry them naturally, put them in a glass bottle, and inoculate 20 yellow striped flea beetle adults of the same size, and seal them with gauze. After the test, transfer them to a constant temperature incubator at 25±2 ^o C and relative humidity of 70%-80% for breeding. The control treatment is treated with clean water without the drug as a blank control. Check the test results 48 hours after the drug treatment. If there is no reaction when touching it with a brush, it is considered dead. If the control mortality rate is greater than 20%, repeat the test.

Calculation method:

Mortality rate (%) = (number of live worms before medication - number of live worms after medication) / number of live worms before medication × 100

Control effect % = ((mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)) × 100

Table 6 Test of the combination of compound I and compound II against yellow striped flea beetle

The test results in Tables 1 to 6 show that the combination of the compound of formula I and compound II achieves a synergistic effect in controlling cabbage aphids, brown planthoppers, rice leaf folders, striped stem borers, diamondback moths, and yellow striped flea beetles.

Claims (15)

1. A pesticidal composition, which contains active ingredient formula I compound and compound II: Compound of formula I

, Compound II is selected from trifluoropyrimidine, and the weight ratio of the compound of formula I to compound II is 10:1-1:50. 2. The insecticidal composition according to claim 1, wherein the weight ratio of the compound of formula I to compound II is 10:1-1:15. 3. The insecticidal composition according to claim 1, wherein the weight ratio of the compound of formula I to compound II is 10:1-1:10. 4. The insecticidal composition according to claim 1, characterized in that, the weight ratio of the compound of formula I to compound II is 5:1-1:5. 5. The insecticidal composition according to claim 1, further comprising a surfactant and/or a filler. 6. The pesticidal composition according to claim 1, wherein the weight of the compound of formula I and compound II accounts for 5%-80% by weight of the pesticidal composition in total. 7. The pesticidal composition according to claim 1, wherein the weight of the compound of formula I and compound II accounts for 5%-70% by weight of the pesticidal composition in total. 8. The pesticidal composition according to claim 1, wherein the weight of the compound of formula I and compound II accounts for 5%-60% by weight of the pesticidal composition in total. 9. The pesticidal composition according to claim 1, wherein the weight of the compound of formula I and compound II accounts for 5%-50% by weight of the pesticidal composition in total. 10. insecticidal composition according to claim 1, is characterized in that, described insecticidal composition, its dosage form is emulsifiable concentrate, water suspension concentrate, oil suspension concentrate, water dispersible granule, wettable powder, suspoemulsion, Emulsion in water, microcapsule suspension, granule or microparticle. 11. The use of the insecticidal composition according to claim 1 for preventing or controlling red aphids, diamondback moths, rice stem borers, rice leaf rollers, brown planthoppers, flea beetle pests and their larvae and eggs. 12. The use according to claim 11, characterized in that the pesticidal composition according to claim 1 is used to act on target useful plants, target pests or their environment, and propagation materials of target useful plants. 13. The use according to claim 11, characterized in that the pesticidal composition according to claim 1 is applied to the seeds of the target useful plants, the target useful plants or the soil where the plants grow or the soil suitable for plant growth. 14. The use according to claim 11, characterized in that, the insecticidal composition according to claim 1 is applied to the soil and/or directly to the plant roots in contact with the plant roots before germination, after germination or before and after germination. Soil or soil suitable for plant growth. 15. A method for protecting seeds from pests and their larvae and eggs, wherein the seeds are contacted with the insecticidal composition of claim 1 in an effective amount before sowing and/or after germination, and The pests are green peach aphid, diamondback moth, rice stem borer, rice leaf roller, brown planthopper, and flea beetle.