CN112557323B

CN112557323B - Spectrophotometer and gas detection method

Info

Publication number: CN112557323B
Application number: CN202011412367.6A
Authority: CN
Inventors: 殷炜晟; 朱丹丹; 施文莉; 崔瑞华; 赵彬
Original assignee: Yin Guosong
Current assignee: Jiangsu Qiuhong Environmental Testing Co ltd
Priority date: 2020-12-04
Filing date: 2020-12-04
Publication date: 2023-03-10
Anticipated expiration: 2040-12-04
Also published as: CN112557323A

Abstract

The spectrophotometer comprises a shell, a darkroom bin is arranged on the shell, a darkroom cover is covered on the darkroom bin, a light source part is arranged on one side wall of the darkroom bin, light receivers are arranged on the opposite side wall of the darkroom bin, a containing frame is arranged in the darkroom bin, a plurality of cuvette placing pipes are circumferentially arranged on the containing frame, and an adjusting component for driving the containing frame to horizontally rotate is arranged on the shell; the detection method adopts a spectrophotometer and comprises the following steps: placing the cuvettes in the cuvette placing tube in sequence, and covering the dark chamber cover; the holding frame is rotated through the adjusting component, so that the cuvette passes through the light source part and the light receiver in sequence, and the absorbance detection is carried out on the sample in the cuvette. This application has the advantage that improves experiment operating efficiency when reducing reagent spill pollution instrument possibility.

Description

Spectrophotometer and gas detection method

Technical Field

The application relates to the technical field of gas detection, in particular to a spectrophotometer and a gas detection method.

Background

Along with the attention of people to the environment, the detection of harmful gas content in the air is more and more common, and a gas detector is generally used for detecting the components and the content of sampled gas. A commonly used gas detector is a spectrophotometer, which is a device for measuring an absorption spectrum. As the spectrophotometer, there are a visible ultraviolet spectrophotometer for measuring a spectrum from a near infrared region to a visible region and a ultraviolet region, an infrared spectrophotometer for measuring a spectrum in an infrared region, and the like.

Related art see chinese patent application with application publication No. CN104508457A, which discloses a spectrophotometer including an optical system part having a light source; a sample holding section which is provided adjacent to the optical system section and holds a sample; and a housing that houses the optical system unit and the sample holding unit.

With respect to the related art among the above, the inventors consider that the following drawbacks exist: when the absorbance value of the sample is detected, the sample may spill to pollute the instrument during the operation of taking and placing the cuvette.

Disclosure of Invention

In order to improve the problem that the instrument is polluted by scattering the sample of the cuvette when the sample is taken and put in the detection process, the application provides a spectrophotometer and a gas detection method.

In a first aspect, the present application provides a spectrophotometer, which adopts the following technical scheme:

the utility model provides a spectrophotometer, includes the casing, is equipped with the darkroom storehouse on the casing, and darkroom storehouse upper cover has closed the darkroom lid, and a lateral wall in darkroom storehouse is equipped with light source portion, and the relative lateral wall in darkroom storehouse is equipped with photoreceiver, be equipped with in the darkroom storehouse and hold the frame, hold the frame and be equipped with a plurality of cuvettes along circumference and place the pipe, the casing is installed the drive and is held a horizontal pivoted adjusting part.

By adopting the technical scheme, after basic preparation work is done, the cuvettes filled with distilled water and different concentrations can be placed into the corresponding cuvette placing tubes at one time, and the dark chamber cover is covered. The containing frame is driven to rotate through the adjusting assembly, so that the samples with different concentrations can be subjected to absorbance detection continuously, the times of taking and placing the cuvette are reduced, and the possibility of polluting an instrument by dropping the samples is greatly prevented.

Optionally, the adjusting assembly includes a chassis and a light-tight light shield disposed along a circumferential direction of the chassis, and a plurality of light-transmitting openings are disposed on the light shield along a diameter direction of the chassis.

Through adopting above-mentioned technical scheme, because the light shield is opaque, when the cell did not remove light source portion position, the light shield played the effect of disconnected light source, and what optical receiver received is the light of penetrating the cell directly, improves the experiment accuracy.

Optionally, a light-transmitting column is vertically arranged on the shell, the chassis and the light-transmitting column are coaxially arranged and rotate relatively, a light-transmitting hole is formed in the light-transmitting column, and the light source portion, the light receiver and the light-transmitting hole are located on the same straight line.

Through adopting above-mentioned technical scheme, the light trap has further reduced the influence of other light to the experimental result, improves the experiment accuracy.

Optionally, the adjusting assembly includes a gear fixed at the center of the chassis and a worm engaged with the gear, the worm is horizontally and rotatably connected to the housing, and one end of the worm penetrates through the housing.

By adopting the technical scheme, the worm is rotated by hands, the worm drives the gear to rotate, and the chassis which is coaxially fixed with the gear rotates. The adjusting assembly is simple in structure and stable in transmission, and the possibility that the sample is scattered along with the rotation of the base plate of the cuvette is reduced.

Optionally, the light-transmitting openings are a plurality of and divided into a group two by two, and the cuvette holding tube is located at one of the light-transmitting openings of each group.

Through adopting above-mentioned technical scheme, the light-admitting opening further plays the effect of sheltering from to the light that the non-straight line was jeted into.

Optionally, the cuvette holding tube is clamped with the light shielding cover.

Through adopting above-mentioned technical scheme, after the experiment, can place the pipe with the cell and take off together, the cell is located the cell and places intraductally, has in time that the sample spills to be placed the pipe by the cell and catches. In addition, when the cuvette placing tube is worn and abraded after being used for a period of time to influence the experiment precision, the cuvette placing tube can be replaced.

Optionally, the chassis is provided with a positioning groove, and the shell is provided with a positioning assembly which is matched with the positioning groove to limit the rotation of the chassis; when the positioning assembly is matched with the positioning groove, one cuvette placing tube is positioned on a straight line connecting line between the light source part and the light receiver.

Through adopting above-mentioned technical scheme, through rotating the worm, can confirm whether the cell moves required position according to application of force size.

Optionally, the shell is provided with a containing hole, the positioning assembly comprises a positioning column and an elastic piece, one end of the elastic piece is fixed with the containing hole, the other end of the elastic piece is fixed with the positioning column, and one end, away from the elastic piece, of the positioning column is a spherical end.

By adopting the technical scheme, when the chassis rotates, the positioning column is extruded and then the compression spring moves towards the accommodating hole, the positioning column is separated from the positioning groove, and the resistance of the rotating worm is small; when the chassis rotates to the position of the positioning groove to the positioning column, the positioning column is propped against the positioning groove again under the action of the spring, and the resistance of the rotating worm is increased suddenly.

Optionally, the darkroom cover is provided with at least one liquid injection pipe, and the liquid injection pipe is positioned above the circumferential motion track of the cuvette holding pipe.

Through adopting above-mentioned technical scheme, can place the cell in the cell is placed intraductally earlier, then utilize and annotate the liquid pipe and add reagent, avoided liquid to spill in the cell.

In a second aspect, the present application provides a gas detection method, which adopts the following technical scheme:

a gas detection method applying the spectrophotometer comprises the following steps:

s1: placing the cuvettes in the cuvette placing tube in sequence, and covering the dark chamber cover;

s2: the holding frame is rotated through the adjusting component, so that the cuvette passes through the light source part and the light receiver in sequence, and the absorbance detection is carried out on the sample in the cuvette.

In summary, the present application includes at least one of the following beneficial technical effects: after opening the darkroom lid, can once only put the cell in and hold the frame, the lid closes the darkroom lid back, can the continuity carry out absorbance detection to the sample in the cell of difference, when having reduced the unrestrained contaminated instrument possibility of reagent, improves experiment operating efficiency.

Drawings

FIG. 1 is a schematic overall structure diagram of an embodiment of the present application;

FIG. 2 isbase:Sub>A sectional view of the structure of the holding frame and the adjusting assembly taken along the line A-A in FIG. 1;

FIG. 3 is an enlarged view of the relative positions of the feed tube and the cuvette taken along the line B-B in FIG. 1;

FIG. 4 is a top view of the holding rack;

fig. 5 is an enlarged view of a portion C of fig. 2 showing the structure of the positioning member.

Description of the reference numerals: 1. a housing; 11. operating a control screen; 12. a dark chamber cover; 121. a card slot; 122. a dust cover; 13. a darkroom cabin; 131. mounting grooves; 14. a light source unit; 15. an optical receiver; 16. A liquid injection pipe; 17. an accommodation hole; 2. a containing frame; 21. a chassis; 211. positioning a groove; 22. a light shield; 221. a light-transmitting opening; 3. an adjustment assembly; 31. a worm; 311. a handle; 32. a gear; 4. a cuvette holding tube; 5. a light-transmitting pillar; 51. a light-transmitting hole; 6. a positioning assembly; 61. a positioning column; 62. an elastic member; 7. and (4) a liquid transferring gun.

Detailed Description

The present application is described in further detail below with reference to figures 1-5.

The embodiment of the application discloses a spectrophotometer. Referring to fig. 1 and 2, the spectrophotometer includes a housing 1, the housing 1 is provided with an operation control screen 11 and a darkroom chamber 13, one side of the darkroom chamber 13 is provided with a light source part 14, the side wall of the darkroom chamber 13, which is opposite to the light source part 14, is provided with a light receiver 15, and a darkroom cover 12 is covered on the darkroom chamber 13; install in the darkroom storehouse 13 and hold frame 2, hold and be provided with a plurality of cuvettes along circumference on the frame 2 and place pipe 4, install the drive on the casing 1 and hold frame 2 pivoted adjusting part 3. The cell that will contain the sample is placed in the cell and is placed pipe 4, starts light source portion 14, rotates through adjusting part 3 and holds frame 2, holds the cell on the frame 2 and passes through between light source portion 14 and the light receiver 15 in proper order to continuity under the condition that can not open dark room cover 12 detects.

Referring to fig. 2 and 3, the shelf 2 includes a bottom plate 21 horizontally disposed and a light-shielding cover 22 fixedly disposed along the periphery of the bottom plate 21. The chassis 21 is a disc, and a through hole is formed in the center of the disc. The light-shielding cover 22 is an opaque ring, the light-shielding cover 22 can be made of glass, and two sides of the glass are rough surfaces. In order to improve the light-blocking effect, a metal foil is fixed on the surface of the light-blocking mask 22. The light-shielding cover 22 can also be made of PVC, and the surface of the light-shielding cover 22 is coated with black paint, so that the light-shielding effect is good. The light-blocking cover 22 has a plurality of light-transmitting openings 221 uniformly formed along the circumferential direction, and the light-transmitting openings 221 may be rectangular openings. The plurality of light-transmitting openings 221 are grouped into two groups, and the two light-transmitting openings 221 in the same group are located at two ends of the light-blocking cover 22 with the same diameter. The cuvette holding tube 4 is fixed to one of the light-transmitting openings 221 of the same group.

The cuvette holding tube 4 can be made of colorless transparent glass or a transparent polyurethane plate and has good transmittance. In order to improve the installation steadiness that the pipe 4 was placed to the cell, the cell was placed the relative both sides wall of pipe 4 and is equipped with the slot, and the slot cooperatees with light shield 22 and is located light-permeable opening 221 department lateral wall, improves the cell and places the fastness of pipe 4. A groove matched with the contour of the cuvette placing pipe 4 can be formed on the bottom plate 21.

Referring to fig. 2 and 4, a boss is integrally formed in the middle of the bottom plate 21, and a through hole in the center of the bottom plate 21 penetrates through the boss. The bottom wall of the casing 1 located in the darkroom chamber 13 is provided with an installation groove 131, and the convex column is located in the installation groove 131. Wear to be equipped with light transmission post 5 in the through-hole, the one end of light transmission post 5 passes through screw fixed connection with casing 1, and the other end of light transmission post 5 exceeds chassis 21, and light transmission post 5 is close to the uncovered one end level of darkroom storehouse 13 and wears to be equipped with light trap 51, and the diameter of light trap 51 is received by light receiver 15 after passing through light trap 51 for the light that light source portion 14 sent. The light-transmitting column 5 and the chassis 21 can be in bearing connection or in rotation connection through grease lubrication.

The adjusting component comprises a gear 32 coaxially sleeved and fixed on the convex column and a worm 31 meshed with the gear 32, the worm 31 is horizontally arranged and penetrates out of the shell 1, the worm 31 is rotatably connected with the shell 1, and a handle 311 is fixed at the end of the worm 31 positioned outside the shell 1. The worm 31 is rotated by rotating the handle 311, and the worm 31 drives the gear 32 to rotate, thereby rotating the chassis 21 around the light-transmitting column 5.

Referring to fig. 2 and 5, in order to rotate the handle 311 to accurately move the cuvette holding tube 4 to the position of the light spot of the light source 14, a hemispherical positioning groove 211 is provided right below the bottom plate 21 corresponding to the center of the groove, and a positioning assembly 6 engaged with the positioning groove 211 is provided on the housing 1.

A receiving hole 17 is provided in the housing 1, and the positioning member 6 is fitted in the receiving hole 17. The positioning assembly 6 includes a positioning post 61 and an elastic member 62, the elastic member 62 may be a spring, one end of the spring is fixed to the bottom wall of the accommodating hole 17, and the other end of the spring is fixed to the positioning post 61. One end of the positioning post 61 away from the elastic element 62 is a spherical end, and the spherical end of the positioning post 61 can extend into the positioning groove 211. When the chassis 21 rotates, the positioning column 61 is extruded and then the compression spring moves towards the accommodating hole 17, the positioning column 61 is separated from the positioning groove 211, and the resistance of the rotating handle 311 is small; when the chassis 21 rotates to the position of the positioning slot 211 to the positioning column 61, the positioning column 61 again abuts against the positioning slot 211 under the action of the spring, and the resistance of the rotating handle 311 is suddenly increased.

Referring to fig. 1 and 4, in order to reduce the possibility of spilling the cuvette holding the sample, a liquid injection tube 16 is fixed to the dark room cover 12. When one of the cuvette holding tubes 4 is moved to face the light-transmitting hole 51 of the light-transmitting column 5, the adjacent cuvette holding tube 4 is positioned directly below the liquid inlet tube 16. The bottom end of the liquid injection pipe 16 is 3-5mm away from the nozzle of the cuvette placing pipe 4, so that the possibility of splashing of the liquid dripped from the liquid injection pipe 16 is reduced. The inner diameter of the distal end of the pouring spout 16 gradually increases and becomes conical. The infusion tube 16 is used for connecting an external pipette gun 7. In order to prevent dust from entering the pouring spout 16 and causing pollution, a clamping groove 121 is formed on the darkroom lid 12, and the top end of the pouring spout 16 is positioned in the clamping groove 121. A dust cover 122 is disposed at the slot 121, and the dust cover 122 can be fixed to the slot 121 in a clamping manner or can be rotatably covered on the slot 121.

The method for detecting gas by applying the spectrophotometer comprises the following steps:

1. after preparing according to the operation requirements of the instrument, opening the dark room cover 12, sequentially filling the cuvettes filled with distilled water and samples with different concentrations into the corresponding cuvette placing tubes 4, then sequentially clamping and fixing the cuvette placing tubes 4 at the light transmission openings 221 of the light shield 22, and covering the dark room cover 12; or fill the cell in proper order with the cell and place pipe 4 on chassis 21, cover dark room lid 12, then turning handle 311 for a plurality of cells are placed pipe 4 and are annotated liquid pipe 16 in proper order, through pipetting gun 7 with distilled water and the sample of different concentrations add the cell that corresponds one by one, and sample concentration increases gradually, covers and closes and hides dirt lid 122.

2. The handle 311 is rotated to rotate the cuvette containing the distilled water to the light source unit 14, and calibration is performed.

3. Then, the handle 311 is rotated to bring the remaining cuvettes with different concentrations to the light source 14 in sequence, and the absorbance is detected.

4. After the detection is finished, the dark chamber cover 12 is opened, and the cuvette accommodating tube 4 is taken out together.

The above are preferred embodiments of the present application, and the scope of protection of the present application is not limited thereto, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. The utility model provides a spectrophotometer, includes casing (1), is equipped with darkroom storehouse (13) on casing (1), and darkroom storehouse (13) upper cover has closed darkroom lid (12), and a lateral wall in darkroom storehouse (13) is equipped with light source portion (14), and the relative lateral wall in darkroom storehouse (13) is equipped with light receiver (15), its characterized in that: a containing frame (2) is arranged in the darkroom bin (13), a plurality of cuvette placing tubes (4) are arranged on the containing frame (2) along the circumferential direction, and an adjusting assembly (3) for driving the containing frame (2) to horizontally rotate is arranged on the shell (1);

the adjusting assembly (3) comprises a chassis (21) and a lighttight light shield (22) arranged along the circumferential direction of the chassis (21), and a plurality of light transmission openings (221) are formed in the light shield (22) along the diameter direction of the chassis (21);

the light-transmitting column (5) is vertically arranged on the shell (1), the chassis (21) and the light-transmitting column (5) are coaxially arranged and relatively rotate, the light-transmitting column (5) is provided with a light-transmitting hole (51), and the light source part (14), the light receiver (15) and the light-transmitting hole (51) are on the same straight line;

the cross section of the cuvette placing tube (4) is rectangular, and two opposite light receiving side walls of the cuvette placing tube (4) are vertical to the axis of the corresponding light transmission hole (51) during detection;

the plurality of light-transmitting openings (221) are divided into a group, and the cuvette placing tube (4) is positioned at one light-transmitting opening (221) of each group;

the cuvette placing tube (4) is fixedly clamped with the light shielding cover (22), slots are formed in two opposite side walls of the cuvette placing tube (4), the slots are matched with the side walls, located at the light transmitting openings (221), of the light shielding cover (22), and grooves matched with the contour of the cuvette placing tube (4) are formed in the base plate (21);

the chassis (21) is provided with a positioning groove (211), and the shell (1) is provided with a positioning component (6) which is matched with the positioning groove (211) to limit the rotation of the chassis (21); when the positioning component (6) is matched with the positioning groove (211), one cuvette placing tube (4) is positioned on a straight line connecting the light source part (14) and the light receiver (15);

the shell (1) is provided with an accommodating hole (17), the positioning assembly (6) comprises a positioning column (61) and an elastic piece (62), one end of the elastic piece (62) is fixed with the accommodating hole (17), the other end of the elastic piece (62) is fixed with the positioning column (61), and one end, away from the elastic piece (62), of the positioning column (61) is a spherical end;

be equipped with at least one notes liquid pipe (16) on dark room cover (12), annotate liquid pipe (16) and be located the cell and place pipe (4) circumferential motion orbit top, draw-in groove (121) have been seted up on dark room cover (12), the top of annotating liquid pipe (16) is located draw-in groove (121), draw-in groove (121) department is equipped with hides dirt lid (122), hide dirt lid (122) card and close on draw-in groove (121) in draw-in groove (121) or rotation lid, the mouth of pipe 3-5mm that the cell (4) was placed to the cell is placed to the bottom distance cell of annotating liquid pipe (16).

2. The spectrophotometer of claim 1, wherein: the adjusting component (3) comprises a gear (32) fixed at the center of the chassis (21) and a worm (31) meshed with the gear (32), the worm (31) is horizontally and rotatably connected to the shell (1) and one end of the worm penetrates out of the shell (1).

3. A method for detecting a gas, using the spectrophotometer according to any one of claims 1 or 2, characterized by: the method comprises the following steps:

s1: the cuvettes are sequentially placed in a cuvette placing tube (4), and a dark chamber cover (12) is covered;

s2: the containing frame (2) is rotated through the adjusting component (3), so that the cuvette passes through the light source part (14) and the light receiver (15) in sequence, and the absorbance detection is carried out on the sample in the cuvette.