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CN112505909B - A Confocal Microscope with Easy Scanning Head Replacement - Google Patents

A Confocal Microscope with Easy Scanning Head Replacement Download PDF

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CN112505909B
CN112505909B CN202011375793.7A CN202011375793A CN112505909B CN 112505909 B CN112505909 B CN 112505909B CN 202011375793 A CN202011375793 A CN 202011375793A CN 112505909 B CN112505909 B CN 112505909B
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block
wall
sliding cavity
scanning head
limiting
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CN112505909A (en
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冯宇
马骁萧
付玲
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Huazhong University of Science and Technology
Ezhou Industrial Technology Research Institute of Huazhong University of Science and Technology
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Huazhong University of Science and Technology
Ezhou Industrial Technology Research Institute of Huazhong University of Science and Technology
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    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036Scanning details, e.g. scanning stages

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Abstract

The invention relates to the technical field of microscopic equipment, in particular to a confocal microscope convenient for replacing a scanning head, which comprises a base and the scanning head, wherein the upper end of the base is fixedly connected with an observation table, an electric platform and a supporting rod, the upper end of the supporting rod is fixedly connected with Z-axis focusing equipment, the side wall of the base is fixedly connected with a mounting block, the inner wall of the mounting block is provided with a sliding cavity, one end of the scanning head is provided with a connector, a connecting component fixedly connected with the connector is arranged in the sliding cavity, and the connecting component can be detachably clamped with the mounting block through an unlocking component. The problems that the scanning head and the connecting seat of the existing confocal microscope are inconvenient to install and detach, the scanning head is troublesome to replace or maintain, and the working efficiency is affected when the scanning head is used for a long time are solved.

Description

一种便于更换扫描头的共聚焦显微镜A confocal microscope with easy replacement of scanning head

技术领域technical field

本发明涉及显微设备技术领域,尤其涉及一种便于更换扫描头的共聚焦显微镜。The invention relates to the technical field of microscopic equipment, in particular to a confocal microscope which is convenient for replacing a scanning head.

背景技术Background technique

共聚焦激光扫描显微镜,是近十多年研制成的高光敏度、高分辨率的新型仪器。它以激光为光源,由共聚焦成像扫描系统、电子光学系统和微机图像分析系统组成。Confocal laser scanning microscope is a new instrument with high light sensitivity and high resolution developed in the past ten years. It uses laser light as the light source and consists of confocal imaging scanning system, electron optical system and microcomputer image analysis system.

光束经聚焦后落在样品(组织厚片或细胞)不同深度的微小一点,并作移动扫描,通过电信号彩色显像,可使样品内任何一点的反射光形成的图像,都被准确地接收下来并产生信号,传递到彩色显示器上,再连接微机图像分析系统进行分析处理。After the beam is focused, it lands on a tiny point at different depths of the sample (thick tissue or cell), and moves and scans, and through the electrical signal color imaging, the image formed by the reflected light at any point in the sample can be accurately received down and generate a signal, which is transmitted to a color display, and then connected to a microcomputer image analysis system for analysis and processing.

在现有技术中,共聚焦显微镜的扫描头与连接座之间不便于安装和拆卸,在更换或维修扫描头时较为麻烦,操作人员需要消耗一定的时间和精力将扫描头从连接座上拆下和将扫描头准确的固定在连接座上,导致在在长期使用时影响工作效率,同时还有可能对设备造成损坏。In the prior art, it is inconvenient to install and disassemble between the scanning head and the connecting seat of the confocal microscope. It is troublesome to replace or maintain the scanning head, and the operator needs to spend a certain amount of time and energy to disassemble the scanning head from the connecting seat. Lowering and accurately fixing the scanning head on the connecting seat will affect the work efficiency during long-term use and may cause damage to the equipment.

发明内容Contents of the invention

有鉴于此,有必要提供一种便于更换扫描头的共聚焦显微镜,用以解决现有地共聚焦显微镜的扫描头与连接座之间不便于安装和拆卸,在更换或维修扫描头时较为麻烦,在长期使用时影响工作效率的问题。In view of this, it is necessary to provide a confocal microscope that is easy to replace the scanning head, in order to solve the inconvenient installation and disassembly between the scanning head and the connecting seat of the existing confocal microscope, and it is more troublesome when replacing or maintaining the scanning head , A problem that affects work efficiency during long-term use.

本发明提供一种便于更换扫描头的共聚焦显微镜,包括底座和扫描头,所述底座的上端固定连接有观测台、电动平台以及支撑杆,所述支撑杆的上端固定连接有Z轴聚焦设备,所述底座的侧壁上固定连接有安装块,所述安装块的内壁设有一滑动空腔,所述扫描头一端设有连接头,所述连接头上开设有连接孔,还包括The invention provides a confocal microscope for easy replacement of the scanning head, including a base and a scanning head, the upper end of the base is fixedly connected with an observation platform, an electric platform and a support rod, and the upper end of the support rod is fixedly connected with a Z-axis focusing device , the side wall of the base is fixedly connected with a mounting block, the inner wall of the mounting block is provided with a sliding cavity, one end of the scanning head is provided with a connecting head, the connecting head is provided with a connecting hole, and also includes

连接组件,所述连接组件包括设于滑动空腔内的滑块、回复弹簧、限位块以及连接片,所述滑块31呈环状,所述滑动空腔151包括有一个直径较大的内壁和一个直径较小的内壁,所述滑块31内壁与所述滑动空腔151中直径较小的一侧内壁接触,所述滑块31的外壁则与所述滑动空腔151中直径较大的一侧内壁之间具有间隙,所述滑块的外壁开设有至少一安装槽,所述连接片一端的侧壁设有与连接孔相配合的凸起,所述回复弹簧一端与安装槽的内底壁固定连接,另一端与限位块固定连接,所述限位块与滑动空腔的内壁相接触,所述连接片的另一端与滑块的一侧固定连接,以及A connection assembly, the connection assembly includes a slider, a return spring, a limit block and a connecting piece arranged in the sliding cavity, the slider 31 is ring-shaped, and the sliding cavity 151 includes a larger diameter Inner wall and an inner wall with a smaller diameter, the inner wall of the slider 31 is in contact with the inner wall of the side with a smaller diameter in the sliding cavity 151, and the outer wall of the slider 31 is in contact with the inner wall of the sliding cavity 151 with a smaller diameter. There is a gap between the inner walls of the large side, the outer wall of the slider is provided with at least one installation groove, the side wall at one end of the connecting piece is provided with a protrusion matching the connection hole, and one end of the return spring is connected to the installation groove. The inner bottom wall of the connecting piece is fixedly connected, the other end is fixedly connected with the limit block, the limit block is in contact with the inner wall of the sliding cavity, the other end of the connecting piece is fixedly connected with one side of the slider, and

解锁组件,所述解锁组件包括推块,设于推块一侧的推杆,所述滑动空腔内壁设有环切槽,所述推块设置在环切槽内,所述推杆的一端延伸至安装块外;An unlocking assembly, the unlocking assembly includes a push block, a push rod arranged on one side of the push block, the inner wall of the sliding cavity is provided with a ring cut groove, the push block is arranged in the ring cut groove, and one end of the push rod extend beyond the mounting block;

所述连接片经由凸起卡设于连接孔与连接头固定,当所述限位块滑动卡固于所述环切槽内时,所述扫描头与所述安装块可拆卸式连接。The connecting piece is clamped in the connecting hole and fixed to the connecting head through the protrusion, and when the limiting block is slid and locked in the ring cut groove, the scanning head is detachably connected to the mounting block.

作为优选,所述滑块的外壁还开设有限位槽,所述滑动空腔中直径较大的内壁上设有与限位槽312相匹配的限位杆153,限位杆153平行于所述滑块31的中心轴线且与所述限位槽312卡接配合。具体地,所述推块与限位块的接触面具有相同的锥度。As a preference, the outer wall of the slider is also provided with a limiting groove, and the inner wall with a larger diameter in the sliding cavity is provided with a limiting rod 153 that matches the limiting groove 312, and the limiting rod 153 is parallel to the The central axis of the slider 31 is engaged with the limiting groove 312 . Specifically, the contact surfaces of the push block and the limit block have the same taper.

作为优选,所述限位块上设有滚珠,所述滚珠与滑动空腔的内壁点接触。Preferably, the limiting block is provided with a ball, and the ball is in point contact with the inner wall of the sliding cavity.

作为优选,所述滑动空腔的内底壁与滑块之间设有压缩弹簧。Preferably, a compression spring is provided between the inner bottom wall of the sliding cavity and the slider.

作为优选,所述推杆的一端设有压块,所述压块与所述安装块之间的推杆上套设有限位弹簧。Preferably, a pressure block is provided at one end of the push rod, and a limit spring is sheathed on the push rod between the pressure block and the installation block.

作为优选,所述滑动空腔的内壁设有限位环块,所述限位环块的内径与所述滑块的直径相同。Preferably, the inner wall of the sliding cavity is provided with a limiting ring block, and the inner diameter of the limiting ring block is the same as that of the sliding block.

具体地,所述连接片为弹性金属或橡胶材料制成。Specifically, the connecting piece is made of elastic metal or rubber material.

本发明的有益效果是:The beneficial effects of the present invention are:

(1)本发明提供一种便于更换扫描头的共聚焦显微镜,通过连接片上的凸起卡设于连接孔内,将连接头与连接组件固定连接,再通过连接组件的上的限位块滑动到环切槽位置,回复弹簧将限位块顶起卡设在环切槽内,将连接组件固定在安装块内,需要更换扫描头时,只需要按压推杆,推块与限位块相互挤压,使限位块脱离环切槽的束缚,即可解锁连接组件,结构简单,安装或拆卸方便,可有效地提高工作效率。(1) The present invention provides a confocal microscope that is easy to replace the scanning head. The protrusion on the connecting piece is clamped in the connecting hole, the connecting head is fixedly connected to the connecting assembly, and then slides through the limit block on the connecting assembly. When the position of the ring groove is reached, the return spring will lift the limit block and set it in the ring groove, and fix the connecting component in the installation block. When the scanning head needs to be replaced, only need to press the push rod, and the push block and the limit block will interact with each other. Squeeze to make the limit block free from the shackles of the ring groove, and then the connecting component can be unlocked. The structure is simple, the installation or disassembly is convenient, and the working efficiency can be effectively improved.

(2)本发明提供一种便于更换扫描头的共聚焦显微镜,为了防止滑块在滑动空腔内发生转动,在滑块的外壁开设限位槽,滑动空腔的内壁设置与限位槽相匹配的限位杆,避免限位块不能准确的卡设到环切槽内,将连接头与安装块固定的情况。(2) The present invention provides a kind of confocal microscope that is convenient for replacing the scanning head. In order to prevent the slider from rotating in the sliding cavity, a limit groove is set on the outer wall of the slider, and the inner wall of the slide cavity is set to match the limit groove The matching limit rod avoids the situation that the limit block cannot be accurately snapped into the ring cut groove to fix the connecting head and the installation block.

附图说明Description of drawings

下面结合附图和实施例对本发明进一步说明。The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

图1是本发明一种便于更换扫描头的共聚焦显微镜的结构示意图;Fig. 1 is the structural representation of a kind of confocal microscope that is convenient to replace scanning head of the present invention;

图2是图1中A的放大示意图;Fig. 2 is the enlarged schematic view of A in Fig. 1;

图3是本发明滑块的机构示意图。Fig. 3 is a schematic diagram of the mechanism of the slider of the present invention.

具体实施方式Detailed ways

下面结合附图来具体描述本发明的优选实施例,其中,附图构成本申请一部分,并与本发明的实施例一起用于阐释本发明的原理,并非用于限定本发明的范围。Preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, wherein the accompanying drawings constitute a part of the application and together with the embodiments of the present invention are used to explain the principle of the present invention and are not intended to limit the scope of the present invention.

一种便于更换扫描头的共聚焦显微镜,包括底座1和扫描头2,所述底座1的上端固定连接有观测台11、电动平台12以及支撑杆13,所述支撑杆13的上端固定连接有Z轴聚焦设备14,所述底座1的侧壁上固定连接有安装块15,所述安装块15的内部设有一滑动空腔151,所述扫描头2一端设有连接头21,所述连接头21上开设有连接孔211,还包括A kind of confocal microscope that is convenient to replace scanning head, comprises base 1 and scanning head 2, and the upper end of described base 1 is fixedly connected with observation platform 11, electric platform 12 and support bar 13, and the upper end of described support bar 13 is fixedly connected with Z-axis focusing device 14, a mounting block 15 is fixedly connected to the side wall of the base 1, a sliding cavity 151 is provided inside the mounting block 15, and a connecting head 21 is provided at one end of the scanning head 2, and the connection The head 21 is provided with a connecting hole 211, which also includes

连接组件,所述连接组件包括设于滑动空腔151内的滑块31、回复弹簧32、限位块33以及连接片34,所述滑块31呈环状,所述滑动空腔151包括有一个直径较大的内壁和一个直径较小的内壁,所述滑块31内壁与所述滑动空腔151中直径较小的一侧内壁接触,所述滑块31的外壁则与所述滑动空腔151中直径较大的一侧内壁之间具有间隙,所述滑块31的外壁开设有至少一安装槽311,所述连接片34一端的侧壁设有与连接孔211相配合的凸起341,所述回复弹簧32一端与安装槽311的内底壁固定连接,另一端与限位块33固定连接,所述限位块33与滑动空腔151的内壁相接触,所述连接片34的另一端与滑块31的一侧固定连接,以及A connection assembly, the connection assembly includes a slider 31 located in the sliding cavity 151, a return spring 32, a limit block 33 and a connecting piece 34, the slider 31 is annular, and the sliding cavity 151 includes An inner wall with a larger diameter and an inner wall with a smaller diameter, the inner wall of the slider 31 is in contact with the inner wall of the side with a smaller diameter in the sliding cavity 151, and the outer wall of the slider 31 is in contact with the sliding cavity. There is a gap between the inner walls of the larger diameter side of the cavity 151, the outer wall of the slider 31 is provided with at least one installation groove 311, and the side wall at one end of the connecting piece 34 is provided with a protrusion that matches the connecting hole 211 341, one end of the return spring 32 is fixedly connected to the inner bottom wall of the installation groove 311, and the other end is fixedly connected to the limit block 33, the limit block 33 is in contact with the inner wall of the sliding cavity 151, and the connecting piece 34 The other end of is fixedly connected with one side of slider 31, and

解锁组件,所述解锁组件包括推块41,设于推块41一侧的推杆42,所述滑动空腔151内壁设有环切槽152,所述推块41设置在环切槽152内,所述推杆42的一端延伸至安装块15外;The unlocking assembly, the unlocking assembly includes a push block 41, a push rod 42 arranged on one side of the push block 41, the inner wall of the sliding cavity 151 is provided with a ring cut groove 152, and the push block 41 is arranged in the ring cut groove 152 , one end of the push rod 42 extends outside the mounting block 15;

所述连接片34经由凸起341卡设于连接孔211与连接头21固定,当所述限位块33滑动卡固于所述环切槽152内时,所述扫描头2与所述安装块15可拆卸式连接。The connecting piece 34 is clamped in the connecting hole 211 via the protrusion 341 and fixed to the connecting head 21. When the limiting block 33 is slid and fixed in the ring cut groove 152, the scanning head 2 and the installation Block 15 detachable connection.

在一种具体实施例中,所述滑块31的外壁还开设有限位槽312,所述滑动空腔151中直径较大的内壁上设有与限位槽312相匹配的限位杆153,限位杆153平行于所述滑块31的中心轴线且与所述限位槽312卡接配合。通过设置限位杆153和限位槽312相配合,引导所述滑块31沿限位杆153的长度方向直线滑动,防止滑块31在滑动空腔151内发生转动,导致限位块33不能准确卡设到环切槽152内,无法将连接头21与安装块15固定。In a specific embodiment, the outer wall of the slider 31 is also provided with a limiting groove 312, and the inner wall of the sliding cavity 151 with a larger diameter is provided with a limiting rod 153 matching the limiting groove 312, The limiting rod 153 is parallel to the central axis of the sliding block 31 and engages with the limiting groove 312 . By arranging the stop rod 153 to cooperate with the stop slot 312, the slide block 31 is guided to slide linearly along the length direction of the stop rod 153, preventing the slide block 31 from rotating in the sliding cavity 151, causing the stop block 33 to fail Accurately clamped into the ring cut groove 152, the connector 21 and the mounting block 15 cannot be fixed.

在一种具体实施例中,所述推块41与限位块33的接触面具有相同的锥度5。在解锁时,只需要按压推杆42,使推块41与限位块33相互挤压,使限位块33脱离环切槽152的束缚,设置锥度5不仅可减少按压时的力度,同时可有效地将限位块33从环切槽152推入到滑动空腔151内实现解锁。In a specific embodiment, the contact surfaces of the pushing block 41 and the limiting block 33 have the same taper 5 . When unlocking, you only need to press the push rod 42 to make the push block 41 and the limit block 33 squeeze each other, so that the limit block 33 is free from the constraints of the ring groove 152. Setting the taper 5 can not only reduce the force when pressing, but also can Effectively push the limiting block 33 from the ring cut groove 152 into the sliding cavity 151 to realize unlocking.

在一种具体实施例中,所述限位块33上设有滚珠6,所述滚珠6与滑动空腔151的内壁点接触。通过设置滚珠6,可减少滑块31在滑动空腔151内滑动时,限位块33与滑动空腔151内壁的摩擦力。In a specific embodiment, the limiting block 33 is provided with a ball 6 , and the ball 6 is in point contact with the inner wall of the sliding cavity 151 . By arranging the balls 6, the friction force between the limiting block 33 and the inner wall of the sliding cavity 151 can be reduced when the sliding block 31 slides in the sliding cavity 151 .

在一种具体实施例中,所述滑动空腔151的内底壁与滑块31之间设有压缩弹簧7。设置压缩弹簧7,当限位块33卡设在环切槽152内时,压缩弹簧7挤压滑块31,使限位块33与环切槽152的内壁紧密贴合,可防止扫描头2与底座1连接松动的情况。In a specific embodiment, a compression spring 7 is provided between the inner bottom wall of the sliding cavity 151 and the slider 31 . Compression spring 7 is set, and when limit block 33 is stuck in ring cut groove 152, compression spring 7 squeezes slide block 31, makes stop block 33 and the inner wall of ring cut groove 152 fit closely, can prevent scanning head 2 In case of loose connection with base 1.

在一种具体实施例中,所述推杆42的一端设有压块43,所述压块43与安装块15外表面之间的推杆42上套设有限位弹簧44。通过设置限位弹簧44,在无外力的作用下,可限制推块41保持在环切槽152内,不会使其底部下落到滑动空腔151中从而阻挡在滑块31的滑动路径上。In a specific embodiment, one end of the push rod 42 is provided with a pressure block 43 , and a limit spring 44 is sleeved on the push rod 42 between the pressure block 43 and the outer surface of the mounting block 15 . By setting the limit spring 44 , under the action of no external force, the push block 41 can be limited to be kept in the circumscribed groove 152 , so that the bottom of the push block 41 will not fall into the sliding cavity 151 and block the sliding path of the slide block 31 .

在一种具体实施例中,所述滑动空腔151中直径较大的内壁上设有限位环块154,所述限位环块154位于远离压缩弹簧7的一端,所述限位环块154的内径与所述滑块31的外壁直径相同。通过设置限位环块154,可防止连接组件从滑动空腔151内滑脱。In a specific embodiment, a limit ring block 154 is provided on the inner wall with a larger diameter in the sliding cavity 151, and the limit ring block 154 is located at an end away from the compression spring 7, and the limit ring block 154 The inner diameter is the same as the outer wall diameter of the slider 31 . By setting the limit ring block 154 , the connecting assembly can be prevented from slipping out of the sliding cavity 151 .

在一种具体实施例中,所述连接片34为弹性金属或橡胶材料制成。In a specific embodiment, the connecting piece 34 is made of elastic metal or rubber material.

工作原理:通过连接片34上的凸起341卡设于连接孔211内来使连接头21与连接组件固定,水平推动扫描头2,使连接组件在滑动空腔151内滑动,当限位块33滑动至环切槽152的位置时,回复弹簧32将限位块33顶起卡设于环切槽152内,阻止连接组件在滑动空腔151内继续滑动,将扫描头2与底座1固定;需要更换扫描头2时,通过推杆42按压推块41,使得推块41挤压限位块33压缩回复弹簧32,使限位块33与环切槽152分离即可。Working principle: The connecting head 21 and the connecting component are fixed by the protrusion 341 on the connecting piece 34 being clamped in the connecting hole 211, and the scanning head 2 is pushed horizontally to make the connecting component slide in the sliding cavity 151, acting as a limit block 33 slides to the position of the ring cut groove 152, the return spring 32 pushes up the limit block 33 and locks it in the ring cut groove 152, preventing the connecting component from continuing to slide in the sliding cavity 151, and fixing the scanning head 2 and the base 1 When the scanning head 2 needs to be replaced, press the push block 41 through the push rod 42, so that the push block 41 squeezes the limit block 33 and compresses the return spring 32, so that the limit block 33 is separated from the ring cut groove 152.

以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本、技术领域的技术人员在本发明揭露的技术范围内,可轻易想到的变化或替换,都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, any changes that can be easily conceived by those skilled in the art and technical field within the technical scope disclosed in the present invention Or replacement, all should be covered within the protection scope of the present invention.

Claims (6)

1. The utility model provides a confocal microscope convenient to change scanning head, includes base and scanning head, the upper end fixedly connected with observation platform, electric platform and the bracing piece of base, the upper end fixedly connected with Z axle focusing facilities of bracing piece, fixedly connected with installation piece on the lateral wall of base, its characterized in that, the inner wall of installation piece is equipped with a slip cavity, scanning head one end is equipped with the connector, the connecting hole has been seted up on the connector, still includes
The connecting assembly comprises a sliding block, a restoring spring, a limiting block and a connecting sheet, wherein the sliding block is arranged in a sliding cavity, the sliding block is annular, the sliding cavity comprises an inner wall with a larger diameter and an inner wall with a smaller diameter, the inner wall of the sliding block is in contact with the inner wall of one side with a smaller diameter in the sliding cavity, a gap is reserved between the outer wall of the sliding block and the inner wall of one side with a larger diameter in the sliding cavity, at least one mounting groove is formed in the outer wall of the sliding block, a protrusion matched with the connecting hole is formed in the side wall of one end of the connecting sheet, one end of the restoring spring is fixedly connected with the inner bottom wall of the mounting groove, the other end of the restoring spring is fixedly connected with the limiting block, the limiting block is in contact with the inner wall of the sliding cavity, and the other end of the connecting sheet is fixedly connected with one side of the sliding block
The unlocking assembly comprises a push block, a push rod arranged on one side of the push block, an annular groove is formed in the inner wall of the sliding cavity, the push block is arranged in the annular groove, and one end of the push rod extends out of the mounting block;
the connecting piece is clamped in the connecting hole through the bulge and is fixed with the connector, and when the limiting block is clamped in the annular groove in a sliding manner, the scanning head is detachably connected with the mounting block;
the compression spring is arranged between the inner bottom wall of the sliding cavity and the sliding block, the outer wall of the sliding block is also provided with a limiting groove, the inner wall with the larger diameter in the sliding cavity is provided with a limiting rod matched with the limiting groove, and the limiting rod is parallel to the central axis of the sliding block and is in clamping fit with the limiting groove.
2. The confocal microscope of claim 1, wherein the contact surfaces of the push block and the stopper have the same taper.
3. The confocal microscope of claim 1, wherein the stopper is provided with balls, and the balls are in point contact with the inner wall of the sliding cavity.
4. The confocal microscope of claim 1, wherein a pressing block is arranged at one end of the pushing rod, and a limiting spring is sleeved on the pushing rod between the pressing block and the mounting block.
5. The confocal microscope of claim 1, wherein the inner wall of the sliding cavity is provided with a limiting ring block, and the inner diameter of the limiting ring block is the same as the diameter of the sliding block.
6. A confocal microscope according to claim 1 wherein said connecting tabs are made of a resilient metal or rubber material.
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