CN112501087A - EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products and preparation method and application thereof - Google Patents
EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products and preparation method and application thereof Download PDFInfo
- Publication number
- CN112501087A CN112501087A CN202011540005.5A CN202011540005A CN112501087A CN 112501087 A CN112501087 A CN 112501087A CN 202011540005 A CN202011540005 A CN 202011540005A CN 112501087 A CN112501087 A CN 112501087A
- Authority
- CN
- China
- Prior art keywords
- bacteria
- hundred million
- improving
- intestinal flora
- regulator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 40
- 244000005700 microbiome Species 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 68
- 241000894006 Bacteria Species 0.000 claims abstract description 41
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 229920002261 Corn starch Polymers 0.000 claims abstract description 15
- 229960000892 attapulgite Drugs 0.000 claims abstract description 15
- 239000008120 corn starch Substances 0.000 claims abstract description 15
- 229910052625 palygorskite Inorganic materials 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 239000002131 composite material Substances 0.000 claims abstract description 4
- 238000009360 aquaculture Methods 0.000 claims description 32
- 244000144974 aquaculture Species 0.000 claims description 32
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 14
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 14
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 14
- 241000193749 Bacillus coagulans Species 0.000 claims description 12
- 241000194108 Bacillus licheniformis Species 0.000 claims description 12
- 244000063299 Bacillus subtilis Species 0.000 claims description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 12
- 241000194032 Enterococcus faecalis Species 0.000 claims description 12
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 12
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 12
- 229940054340 bacillus coagulans Drugs 0.000 claims description 12
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 12
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000003124 biologic agent Substances 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 7
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 6
- 239000000047 product Substances 0.000 abstract 2
- 239000013589 supplement Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 description 42
- 230000001580 bacterial effect Effects 0.000 description 12
- 241000371997 Eriocheir sinensis Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Botany (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to an EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products, and a preparation method and application thereof. The preparation method of the EM bacteria regulator comprises the following steps: step 1: uniformly mixing all the bacteria in proportion to obtain an EM microbial inoculum; step 2: firstly, adding corn starch and attapulgite into the composite bacteria composition according to a certain proportion; and step 3: mixing the mixture with water in a mass ratio of 1: 100-1: 1000; and 4, step 4: uniformly stirring for 15 minutes by using a stirrer, and fermenting for 4-6 days at the temperature of 25-32 ℃ to obtain the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products. The invention has sufficient effective bacteria content, stabilizes the PH of the water body, supplements beneficial microorganisms of the water body, keeps the bacteria phase balance of the water body, reduces harmful indexes of ammonia nitrogen, nitrite and the like of the water body, decomposes organic matters and residual baits of the water body, and provides a high-quality water body environment for culture.
Description
Technical Field
The invention relates to an EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products, a preparation method and application thereof, belonging to the technical field of aquaculture.
Background
In intensive aquaculture, the aquaculture water body pollutes seriously. Meanwhile, the quality of the internal and external environments of the aquaculture water body is gradually reduced due to the influence of factors such as industrial and agricultural production, disordered discharge of domestic wastewater and the like. Good water quality is extremely important for the survival and normal growth of aquatic animals. Deterioration of water quality is a major obstacle affecting the survival rate and yield of aquaculture organisms and is also a major cause of outbreaks of aquatic diseases. The outbreak of disease causes the heavy use of chemical drugs such as antibiotics and the residue in animals, which also threatens the health of human beings. Therefore, the purification of aquaculture environment has become a key technology and research hotspot for the continuous development of the aquaculture industry.
With the development of aquaculture industry to high-density and intensive large-scale production, the water quality problem of aquaculture water becomes a key factor which restricts the sustainable development of aquaculture industry in China. The effect of the generally adopted physicochemical method for controlling the water quality is not satisfactory, so that the biological regulation and control by using the microecologics are highly regarded. The EM preparation is prepared by mixing and culturing screened beneficial microorganisms in a proper proportion by a unique fermentation process to form a composite microbial community and beneficial substances and secretions thereof, and a complex and stable microecological system is formed through symbiotic proliferation. Beneficial microorganisms in EM can form various nutrient elements for growth and reproduction of self and bait organisms through a series of decomposition and synthesis actions such as nitrogen fixation, photosynthesis and the like, and meanwhile, dissolved oxygen in water is increased, the content of toxic substances such as ammonia, hydrogen sulfide and the like is reduced, and the water changing times are reduced. Is a microecological preparation which has the widest effective range and is used most.
However, the use effects of the existing EM preparations in the same industry are different, most EM preparations have the problem of serious shortage of effective bacteria content, and the object on the package is seriously inconsistent with the actual filling material, so that the aim of reducing the contents of nitrite, ammonia nitrogen, hydrogen sulfide and the like in the aquaculture water body cannot be achieved. But also can not solve the problem that organic matters in the water body and residual baits artificially fed damage the water body.
Disclosure of Invention
In order to solve the technical problems, the invention provides an EM (effective microorganisms) regulator for improving aquaculture aquatic intestinal flora and a preparation method and application thereof, and the specific technical scheme is as follows:
an EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products comprises EM microbial inoculum consisting of lactobacillus acidophilus, lactobacillus plantarum, enterococcus faecalis, bacillus licheniformis, bacillus subtilis, bacillus coagulans and saccharomycetes, and the distribution density of each component in the EM microbial inoculum is as follows:
further, the biological feed also comprises corn starch and attapulgite, wherein the mass ratio of the EM microbial inoculum to the mixture of the EM microbial inoculum, the corn starch and the attapulgite is as follows: 2-6%.
Further, the mass ratio of the corn starch to the attapulgite is 10: 1-5: 1.
Further, the distribution density in the EM microbial inoculum is as follows:
the preparation method of the EM bacteria regulator for improving the aquaculture aquatic intestinal flora is characterized by comprising the following steps: the method comprises the following steps:
step 1: uniformly mixing lactobacillus acidophilus, lactobacillus plantarum, enterococcus faecalis, bacillus licheniformis, bacillus subtilis, bacillus coagulans and saccharomycetes according to the proportion in claim 1 to obtain an EM microbial inoculum;
step 2: firstly, adding corn starch into the composite bacteria composition in proportion, and then adding attapulgite in proportion to obtain a mixture a;
and step 3: putting the mixture a into a stirring tank, and mixing the mixture a with water according to the mass ratio of 1: 100-1: 1000 to obtain a mixture b;
and 4, step 4: and (3) uniformly stirring the mixture b for 15 minutes by using a V-shaped stirrer or a three-V stirrer, and fermenting for 4-6 days at the temperature of 25-32 ℃ to obtain the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products.
The application of the EM bacteria regulator for improving the aquaculture aquatic intestinal flora is characterized in that: the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products is sprayed to the aquatic product culture water body at the concentration of 3-9 g/mu, and the average depth of the water body is 0.5-1.5 m.
Furthermore, the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products is sprayed on the aquatic product culture water body at the concentration of 5 g/mu, and the depth of the water body is 1m on average.
The invention has the beneficial effects that: the effective bacteria content is sufficient, and the aim of supplementing probiotics can be achieved after fermentation. The PH value of the water body is stabilized, beneficial microorganisms in the water body are supplemented, the bacteria phase balance of the water body is kept, and the problem of insufficient content of the beneficial bacteria in the water body is solved. Reduce harmful indexes such as water ammonia nitrogen, nitrite and the like, decompose organic matters and residual baits in the water body, and provide a high-quality ecological environment for the cultured objects.
Detailed Description
The unit of hundred million/gram in the invention refers to how many hundred million certain bacteria are contained in the mixture a of the EM microbial inoculum for each gram of aquaculture water, for example, the lactobacillus acidophilus is 2-4.5 hundred million/gram, namely, the mixture a of the EM microbial inoculum for each gram of aquaculture water contains 2-4.5 hundred million lactobacillus acidophilus.
The following specific examples are provided to verify the technical effects achieved by the present invention:
each pond is a pond for growing over-mature shrimps and crabs, the water quality conditions are basically the same, and each pond is respectively treated by the following different treatments:
blank comparative example: 20 mu of pond water with the average water depth of 1m without adding any preparation.
Control group: adding common EM microbial inoculum regulator, wherein the EM microbial inoculum accounts for 2kg, the starch accounts for 198kg, then mixing with water according to the mass ratio of 1:100, and then throwing 20 mu of pond water with the average depth of 1m under the same condition.
Example 1: the EM bacteria regulator added into pond water for improving the intestinal flora of aquaculture aquatic products comprises the following components in percentage by weight:
800g of lactobacillus acidophilus with the raw material content of 500 hundred million/g,
800g of lactobacillus plantarum raw material with the content of 500 hundred million/g,
1500g of enterococcus faecalis with the raw material content of 500 hundred million/g,
600g of bacillus licheniformis with the raw material content of 1000 hundred million/g,
800g of raw material of bacillus subtilis with the content of 2000 hundred million/g,
700g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
350g of yeast with the raw material content of 200 hundred million/g,
173kg of corn starch and 20kg of attapulgite.
Example 2: the EM bacteria regulator added into pond water for improving the intestinal flora of aquaculture aquatic products comprises the following components in percentage by weight:
1000g of lactobacillus acidophilus with the raw material content of 500 hundred million/g,
1100g of raw material of lactobacillus plantarum has the content of 500 hundred million/g,
1800g of enterococcus faecalis with the raw material content of 500 hundred million/g,
800g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
1000g of bacillus subtilis with the raw material content of 2000 hundred million/g,
800g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
500g of yeast with the raw material content of 200 hundred million/g,
173kg of corn starch and 20kg of attapulgite.
Example 3: the EM bacteria regulator added into pond water for improving the intestinal flora of aquaculture aquatic products comprises the following components in percentage by weight:
1200g of lactobacillus acidophilus with the raw material content of 500 hundred million/g,
1200g of lactobacillus plantarum with the raw material content of 500 hundred million/g,
2000g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1000g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
1200g of the bacillus subtilis with the raw material content of 2000 hundred million/g,
1000g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
600g of yeast with the raw material content of 200 hundred million/g,
170kg of corn starch and 21.8kg of attapulgite.
Example 4: the EM bacteria regulator added into pond water for improving the intestinal flora of aquaculture aquatic products comprises the following components in percentage by weight:
1400g of lactobacillus acidophilus with the raw material content of 500 hundred million/g,
1300g of lactobacillus plantarum with the raw material content of 500 hundred million/g,
2200g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1200g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
1400g of the raw material of the bacillus subtilis with the content of 2000 hundred million/g,
1200g of bacillus coagulans with the raw material content of 1000 hundred million/g,
700g of yeast raw material with the content of 200 hundred million/g,
167.4kg of corn starch and 22.6kg of attapulgite.
Example 5: the EM bacteria regulator added into pond water for improving the intestinal flora of aquaculture aquatic products comprises the following components in percentage by weight:
1600g of lactobacillus acidophilus raw material with the content of 500 hundred million/g,
1400g of lactobacillus plantarum with the raw material content of 500 hundred million/g,
2400g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1400g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
1500g of bacillus subtilis with the raw material content of 2000 hundred million/g,
1400g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
800g of yeast with the raw material content of 200 hundred million/g,
167kg of corn starch and 22.5kg of attapulgite.
Example 6: the EM bacteria regulator added into pond water for improving the intestinal flora of aquaculture aquatic products comprises the following components in percentage by weight:
1800g of lactobacillus acidophilus raw material with the content of 500 hundred million/g,
1500g of lactobacillus plantarum with the raw material content of 500 hundred million/g,
2500g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1500g of Bacillus licheniformis with the raw material content of 1000 hundred million/g,
1500g of bacillus subtilis with the raw material content of 2000 hundred million/g,
1500g of bacillus coagulans with the raw material content of 1000 hundred million/g,
1000g of yeast with the raw material content of 200 hundred million/g,
160.7kg of corn starch and 28kg of attapulgite.
The following are the pond water quality conditions of the examples, as shown in tables 1-2 below:
TABLE 1
TABLE 2
As can be seen from tables 1 and 2, when the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products is not used, the transparency of the water body in the blank comparative example is 10-20cm, and the water quality is turbid. The pH value of the water body is up to 9.2, the dissolved oxygen content of the water body is lower and is 4.3mg/L, the ammonia nitrogen content of the water body is 0.04mg/L, the nitrite content of the water body is 0.18ppm, and the organic matter content is up to 40 g; when the common EM microbial inoculum is added, the transparency is relieved but the effect is not large, the pH value of the water body is not obviously changed, and the ammonia nitrogen content, the nitrite content and the organic matter content of the water body are relieved but not large; after the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products is treated, the transparency of the water body in the examples 1-6 is 22-35cm, the water body is clear obviously, the water quality is good, the PH of the water body is reduced to about 8.2, the PH is obviously lower than that of a blank comparative example, the dissolved oxygen content of the water body is increased, the ammonia nitrogen content of the water body is reduced to be below 0.02mg/L, the nitrite content of the water body is reduced to be below 0.1ppm, and the organic matter content is obviously reduced to be 24-5 g. In aquaculture, the PH of the water body which is most beneficial to the growth of aquatic organisms is about 8.2, the content of ammonia nitrogen is less than 0.02mg/L, the content of nitrite is less than 0.1ppm, and the water body basically meets the requirement by using the EM bacteria regulator for improving the intestinal flora of aquaculture aquatic organisms. Researches show that the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products has good effect of regulating water quality and has good application prospect in the aquaculture.
The eriocheir sinensis is bred in each pond, the eriocheir sinensis generally shells 18 times, the most critical period of the aquaculture is 4 months to 10 months, the eriocheir sinensis generally shells 5 times in the whole period, the preparations in different embodiments are added into one pond respectively, and the following conditions are different preparations:
blank comparative example: no regulator is added, and the feed is normally sprinkled.
Control group: adding common EM microbial agent regulator, and normally sprinkling feed.
Example 1: besides normal feed sprinkling, the EM bacterial regulator for improving the intestinal flora of the cultured aquatic products, which is disclosed by the invention and is in the amount of the embodiment 1, is sprinkled every 10-15 days, namely the EM bacterial regulator disclosed by the invention is sprinkled for 3-4 times in each shelling period.
Example 2: besides normal feed sprinkling, the EM bacterial regulator for improving the intestinal flora of the cultured aquatic products, which is disclosed by the invention and is in the amount of the embodiment 2, is sprinkled every 10-15 days, namely the EM bacterial regulator disclosed by the invention is sprinkled for 3-4 times in each shelling period.
Example 3: besides normal feed sprinkling, the EM bacterial regulator for improving the intestinal flora of the cultured aquatic products, which is disclosed by the invention, is sprayed every 10-15 days, namely the EM bacterial regulator disclosed by the invention is sprayed for 3-4 times in each shelling period.
Example 4: besides normal feed sprinkling, the EM bacterial regulator for improving the intestinal flora of the cultured aquatic products, which is disclosed by the invention, is sprayed in an amount of example 4 every 10-15 days, namely the EM bacterial regulator disclosed by the invention is sprayed for 3-4 times in each shelling period.
Example 5: besides normal feed sprinkling, the EM bacterial regulator for improving the intestinal flora of the cultured aquatic products, which is disclosed by the invention and is in the amount of the embodiment 5, is sprinkled every 10-15 days, namely the EM bacterial regulator disclosed by the invention is sprinkled for 3-4 times in each shelling period.
Example 6: besides normal feed sprinkling, the EM bacterial regulator for improving the intestinal flora of the cultured aquatic products, which is disclosed by the invention and is in the amount of 6, is sprinkled every 10-15 days, namely the EM bacterial regulator disclosed by the invention is sprinkled for 3-4 times in each shelling period.
The average weight of the eriocheir sinensis crabs is measured every 35 days from 4 months to 5 days under water in different ponds so as to measure the growth condition of the eriocheir sinensis crabs:
TABLE 3
The results of the above table 3 show that the blank comparative example is 60g on average in 4 months, hulling is performed every 35 days on average, and the weight of the Eriocheir sinensis is increased by about 25g in each hulling process until the maximum weight is about 160g in 10 months; in contrast, the common EM microbial inoculum is added into the pond water, the weight of the eriocheir sinensis is still about 25g every 35 days, and the weight of the eriocheir sinensis is not obviously increased by 8.29-10.4; in the embodiments 1-6, the EM bacteria regulator for improving the aquaculture aquatic product intestinal flora is added, the weight can be increased by about 40g every 35 days, and the maximum weight is about 290g in 10 months. Much heavier than the blank comparative example and the control example. The EM bacteria regulator for improving the aquaculture aquatic intestinal flora has a great promotion effect on the growth of the mitten crabs. Meanwhile, the resistance of the eriocheir sinensis can be enhanced. Compared with the common EM microbial inoculum, the regulator of the invention has more obvious effect and good application prospect.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (7)
1. An EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products is characterized in that: the microbial inoculum comprises an EM microbial inoculum consisting of lactobacillus acidophilus, lactobacillus plantarum, enterococcus faecalis, bacillus licheniformis, bacillus subtilis, bacillus coagulans and saccharomycetes, and the distribution density of each component in the EM microbial inoculum is as follows:
2-4.5 hundred million/g of lactobacillus acidophilus,
2-3.75 hundred million/g of lactobacillus plantarum,
3.75-6.25 hundred million/g enterococcus faecalis,
3-7.5 hundred million/g of Bacillus licheniformis,
8-15 hundred million/g of bacillus subtilis,
3.5 to 7.5 hundred million/g of Bacillus coagulans,
0.35-1 hundred million/g of yeast.
2. An EM bacteria modulator for improving the intestinal flora of a cultured aquaculture as claimed in claim 1 wherein: the biological agent also comprises corn starch and attapulgite, wherein the mass ratio of the EM microbial agent to the mixture of the EM microbial agent, the corn starch and the attapulgite is as follows: 2-6%.
3. An EM bacteria modulator for improving the intestinal flora of a cultured aquaculture as claimed in claim 2 wherein: the mass ratio of the corn starch to the attapulgite is 10: 1-5: 1.
4. An EM bacteria modulator for improving the intestinal flora of a cultured aquaculture as claimed in claim 1 wherein: wherein, the distribution density in the EM microbial inoculum is as follows:
3 hundred million/g of lactobacillus acidophilus,
2.75 hundred million/g of lactobacillus plantarum,
the enterococcus faecalis is 5 hundred million/gram,
the bacillus licheniformis is 4 hundred million/gram,
10 hundred million/g of bacillus subtilis,
the bacillus coagulans is 4 hundred million/gram,
0.5 hundred million/g yeast.
5. The method for preparing the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products as claimed in claim 2, wherein the EM bacteria regulator comprises: the method comprises the following steps:
step 1: uniformly mixing lactobacillus acidophilus, lactobacillus plantarum, enterococcus faecalis, bacillus licheniformis, bacillus subtilis, bacillus coagulans and saccharomycetes according to the proportion in claim 1 to obtain an EM microbial inoculum;
step 2: firstly, adding corn starch into the composite bacteria composition in proportion, and then adding attapulgite in proportion to obtain a mixture a;
and step 3: putting the mixture a into a stirring tank, and mixing the mixture a with water according to the mass ratio of 1: 100-1: 1000 to obtain a mixture b;
and 4, step 4: and (3) uniformly stirring the mixture b for 15 minutes by using a V-shaped stirrer or a three-V stirrer, and fermenting for 4-6 days at the temperature of 25-32 ℃ to obtain the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products.
6. The use of an EM bacteria modulator for improving the intestinal flora of a cultured aquatic product, prepared according to claim 5, wherein the EM bacteria modulator is characterized in that: the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products is sprayed to the aquatic product culture water body at the concentration of 3-9 g/mu, and the average depth of the water body is 0.5-1.5 m.
7. Use of a modulator of EM bacteria for improving the intestinal flora of aquaculture as claimed in claim 6 wherein: the EM bacteria regulator for improving the intestinal flora of the cultured aquatic products is sprayed on the aquatic product culture water body at the concentration of 5 g/mu, and the average depth of the water body is 1 m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011540005.5A CN112501087A (en) | 2020-12-23 | 2020-12-23 | EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011540005.5A CN112501087A (en) | 2020-12-23 | 2020-12-23 | EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112501087A true CN112501087A (en) | 2021-03-16 |
Family
ID=74923197
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011540005.5A Pending CN112501087A (en) | 2020-12-23 | 2020-12-23 | EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112501087A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113402034A (en) * | 2021-05-27 | 2021-09-17 | 福州一诺维生物科技有限公司 | Intestinal flora regulator for aquaculture and preparation method thereof |
| CN113897311A (en) * | 2021-09-30 | 2022-01-07 | 武汉华扬天乐生物科技有限公司 | A kind of self-cultivation compound bacteria and its preparation method and application |
| CN115432826A (en) * | 2021-06-04 | 2022-12-06 | 亿盛(阳江)生物技术有限公司 | Microbial regulator and application method thereof |
| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101323843A (en) * | 2008-07-25 | 2008-12-17 | 浙江大学 | Composite bacillus preparation and preparation method for improving seawater aquaculture water environment |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN102757131A (en) * | 2012-07-30 | 2012-10-31 | 宜春强微生物科技有限公司 | Water quality ameliorant for aquaculture |
| CN103224886A (en) * | 2013-04-24 | 2013-07-31 | 武汉市天辰生物科技有限公司 | Composite microbe inocula for aquatic product culture and application thereof |
| CN108865935A (en) * | 2018-07-05 | 2018-11-23 | 济宁滨阳生物科技股份有限公司 | A kind of probiotics of water quality improvement and preparation method thereof |
| CN108996711A (en) * | 2018-09-11 | 2018-12-14 | 江苏远山生物技术有限公司 | A kind of compound micro-ecological preparation used for aquiculture and preparation method thereof containing clostridium butyricum |
| CN109321505A (en) * | 2018-11-16 | 2019-02-12 | 众合发(北京)生物科技发展有限公司 | A kind of complex microorganism preparations adjusting aquatic livestock enteron aisle |
| CN109607811A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of apostichopus japonicus culture |
| CN110257292A (en) * | 2019-06-27 | 2019-09-20 | 中国科学院烟台海岸带研究所 | A kind of microbial agent for aquaculture and preparation method thereof |
-
2020
- 2020-12-23 CN CN202011540005.5A patent/CN112501087A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101323843A (en) * | 2008-07-25 | 2008-12-17 | 浙江大学 | Composite bacillus preparation and preparation method for improving seawater aquaculture water environment |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN102757131A (en) * | 2012-07-30 | 2012-10-31 | 宜春强微生物科技有限公司 | Water quality ameliorant for aquaculture |
| CN103224886A (en) * | 2013-04-24 | 2013-07-31 | 武汉市天辰生物科技有限公司 | Composite microbe inocula for aquatic product culture and application thereof |
| CN108865935A (en) * | 2018-07-05 | 2018-11-23 | 济宁滨阳生物科技股份有限公司 | A kind of probiotics of water quality improvement and preparation method thereof |
| CN108996711A (en) * | 2018-09-11 | 2018-12-14 | 江苏远山生物技术有限公司 | A kind of compound micro-ecological preparation used for aquiculture and preparation method thereof containing clostridium butyricum |
| CN109321505A (en) * | 2018-11-16 | 2019-02-12 | 众合发(北京)生物科技发展有限公司 | A kind of complex microorganism preparations adjusting aquatic livestock enteron aisle |
| CN109607811A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of apostichopus japonicus culture |
| CN110257292A (en) * | 2019-06-27 | 2019-09-20 | 中国科学院烟台海岸带研究所 | A kind of microbial agent for aquaculture and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113402034A (en) * | 2021-05-27 | 2021-09-17 | 福州一诺维生物科技有限公司 | Intestinal flora regulator for aquaculture and preparation method thereof |
| CN115432826A (en) * | 2021-06-04 | 2022-12-06 | 亿盛(阳江)生物技术有限公司 | Microbial regulator and application method thereof |
| CN115432826B (en) * | 2021-06-04 | 2024-08-20 | 亿盛(阳江)生物技术有限公司 | Microbial regulator and application method thereof |
| CN113897311A (en) * | 2021-09-30 | 2022-01-07 | 武汉华扬天乐生物科技有限公司 | A kind of self-cultivation compound bacteria and its preparation method and application |
| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112501087A (en) | EM (effective microorganisms) regulator for improving intestinal flora of cultured aquatic products and preparation method and application thereof | |
| CN108913632B (en) | Compound microorganism fermentation liquor for aquaculture and preparation method and application thereof | |
| CN101643303B (en) | Method for treating pigsty pollution by fermenting pig excrement with zymogencous microecological preparations | |
| CN104803485A (en) | Microorganism compound enzyme water purifying agent particle | |
| CN102910715B (en) | Water quality regulation method for high-position culture pond of prawns | |
| CN102550826B (en) | Active dry saccharomyces boulardii feed additive and its preparation method and use | |
| CN101269884A (en) | Microorganism renovation agent of water environment and preparation method thereof | |
| CN111187731A (en) | A kind of bio-improving bottom-cultivating algae water-quality improving bacterial agent and preparation method thereof | |
| CN108660090A (en) | A kind of complex microorganism preparations used for aquiculture and method | |
| CN110627564A (en) | Biological fish manure for aquaculture and preparation method thereof | |
| CN110982750A (en) | High-density fermentation method for rhodopseudomonas palustris and application of high-density fermentation method | |
| CN105085088A (en) | Water fertilizing paste for cultivating green algae in aquaculture pond | |
| CN110915735B (en) | Efficient healthy culture method for prawns | |
| CN103641229B (en) | Method for regulating and controlling of water quality of Litopenaeus Vannamei culture pond | |
| CN103693751B (en) | Attapulgite powder and rice husk make the holothruian cultures Water quality restoration agent of carrier and method for making thereof and purposes | |
| CN111100830B (en) | A kind of composite microecological preparation for aquaculture water purification and its application | |
| CN102919611A (en) | Manufacturing method of fermented bait for larval sea cucumber | |
| CN109133368A (en) | A kind of complex microorganism water purification agent and preparation method thereof for aquaculture | |
| CN113754218A (en) | Tablet of composite bottom modifying bacteria and preparation method thereof | |
| CN107467414A (en) | A kind of compound feed additive of probiotics fermention natural grain | |
| CN112707515A (en) | Water body repairing agent for reducing water body N, P and preparation method and application thereof | |
| CN113213642A (en) | Water-regulating microecological preparation for micropterus salmoides seedling culture and preparation method thereof | |
| CN1962489A (en) | Microecological regulation tablet for aquaculture | |
| CN112616998B (en) | Microbial fermentation feed suitable for penaeus vannamei boone | |
| CN114045228B (en) | Microbial water quality regulator and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210316 |