CN112494577A - Traditional Chinese medicine microecological preparation for treating piglet diarrhea and preparation method thereof - Google Patents
Traditional Chinese medicine microecological preparation for treating piglet diarrhea and preparation method thereof Download PDFInfo
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- CN112494577A CN112494577A CN202011556868.1A CN202011556868A CN112494577A CN 112494577 A CN112494577 A CN 112494577A CN 202011556868 A CN202011556868 A CN 202011556868A CN 112494577 A CN112494577 A CN 112494577A
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Abstract
The invention discloses a traditional Chinese medicine microecological preparation for treating piglet diarrhea and a preparation method thereof. The traditional Chinese medicine microecological preparation is prepared by crushing traditional Chinese medicine decoction piece raw materials, inoculating for the first time, performing solid state fermentation, extracting with water, sterilizing, inoculating for the second time and performing liquid state fermentation, wherein the traditional Chinese medicine decoction piece raw materials comprise the following components in parts by weight: 55-65 parts of Chinese pulsatilla root, 25-35 parts of golden cypress, 40-50 parts of coptis chinensis, 55-65 parts of ash bark, 10-20 parts of rhizoma atractylodis, 40-50 parts of rhizoma alismatis, 40-50 parts of akebia stem, 15-25 parts of dried orange peel, 55-65 parts of astragalus mongholicus, 25-35 parts of codonopsis pilosula, 25-35 parts of bighead atractylodes rhizome, 25-35 parts of poria cocos and 10-20 parts of liquorice, wherein the first inoculation is a mixture of inoculated lactobacillus plantarum strains and saccharomycete strains, and the second inoculation is inoculated lactobacillus plantarum strains. The traditional Chinese medicine microecological preparation can effectively treat the diarrhea of piglets, so that the survival rate and the production performance of the piglets are improved.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly relates to a traditional Chinese medicine microecological preparation for treating piglet diarrhea and a preparation method thereof.
Background
The newborn piglets are easy to have diarrhea due to incomplete development of an autoimmune system and limited body temperature regulation capacity, so that the high death rate is caused, and the great loss is caused to the pig raising industry. The main factors causing the diarrhea of the piglets are maternal-derived factors such as poor quality of breast milk, pathogenic factors such as escherichia coli and epidemic diarrhea virus, poor feeding management and the like. In recent years, the method for treating the diarrhea problem of piglets mainly comprises the steps of injecting antibiotics into piglets and orally taking a traditional Chinese medicine preparation.
Although antibiotics have excellent effects in the aspects of treating piglet diarrhea, improving the production performance, reducing the breeding cost and the like, due to the fact that bacterial drug resistance, environmental pollution and drug residue are increasingly problematic, the call for banning resistance is increasingly rising at present, and the development of antibiotic substitute products is urgent. And the injection treatment easily causes piglet stress reaction to cause piglet death.
Patent application CN201610193208.9 discloses a traditional Chinese medicine soluble powder for treating piglet diarrhea and a preparation method thereof. The traditional Chinese medicine soluble powder for treating piglet diarrhea is mainly prepared by mixing the following components in parts by weight: 20-40 parts of coptis chinensis, 20-40 parts of rhubarb, 20-40 parts of folium isatidis, 10-15 parts of honeysuckle leaves, 5-10 parts of liquorice and 0.1-0.3 part of sweetening agent. The preparation method comprises the steps of cleaning, drying and crushing the components to 40-80 meshes, adding purified water, carrying out ultrasonic extraction, clarifying, filtering, concentrating and spray drying to obtain the tea. However, the treatment effect of the traditional Chinese medicine soluble powder for treating piglet diarrhea is not good enough, and the preparation method has the problem that the traditional Chinese medicine is wasted due to low extraction rate of the effective components of the traditional Chinese medicine decoction pieces.
Disclosure of Invention
The invention aims to: the traditional Chinese medicine microecological preparation for treating piglet diarrhea and the preparation method thereof are provided, aiming at treating piglet diarrhea so as to improve the survival rate and the production performance of piglets, improve the effect of traditional Chinese medicines, expand the function, improve the palatability and solve the problem of traditional Chinese medicine waste caused by low extraction rate of effective components of traditional Chinese medicine decoction pieces.
The technical scheme adopted by the invention is as follows:
a Chinese medicinal microecological preparation for treating diarrhea of piglets. The traditional Chinese medicine microecological preparation for treating piglet diarrhea is prepared by crushing traditional Chinese medicine decoction piece raw materials, primary inoculation, solid fermentation, water extraction, sterilization, secondary inoculation and liquid fermentation, wherein the traditional Chinese medicine decoction piece raw materials comprise the following components in parts by weight: 55-65 parts of Chinese pulsatilla root, 25-35 parts of golden cypress, 40-50 parts of coptis chinensis, 55-65 parts of ash bark, 10-20 parts of rhizoma atractylodis, 40-50 parts of rhizoma alismatis, 40-50 parts of akebia stem, 15-25 parts of dried orange peel, 55-65 parts of astragalus mongholicus, 25-35 parts of codonopsis pilosula, 25-35 parts of bighead atractylodes rhizome, 25-35 parts of poria cocos and 10-20 parts of liquorice, wherein the first inoculation is a mixture of inoculated lactobacillus plantarum strains and saccharomycete strains, and the second inoculation is inoculated lactobacillus plantarum strains.
The traditional Chinese medicine microecological preparation for treating piglet diarrhea has the following characteristics of the raw materials:
the Chinese pulsatilla root: cold in nature, bitter in taste; entering large intestine and stomach meridians; has effects of clearing away heat and toxic materials, cooling blood, and relieving dysentery; it is mainly used for treating diarrhea due to damp-heat, and dysentery with bloody stool due to toxic heat.
Phellodendron bark: cold in nature, bitter in taste; entering kidney and bladder meridians; has effects in clearing away damp-heat, purging pathogenic fire, and relieving deficiency heat; it can be used for treating dysentery due to damp-heat, jaundice, night sweat due to yin deficiency, pyocutaneous disease, and toxic swelling.
Coptis chinensis: cold in nature, bitter in taste; the channels of heart, liver, stomach and large intestine; has effects in clearing away heat, eliminating dampness, purging pathogenic fire, and removing toxic materials; it is indicated for dysentery due to damp-heat, hyperactivity of heart-fire, sores and abscess due to fire-toxin, and conjunctival congestion with swelling and pain.
Cortex fraxini: cold in nature, bitter in taste; it enters liver, gallbladder and large intestine meridians; has the effects of clearing heat, eliminating dampness, clearing liver and improving eyesight, and is mainly used for treating damp-heat diarrhea and dysentery, conjunctival congestion and swelling and pain.
Rhizoma atractylodis: warm in nature, pungent and bitter in flavor; spleen and stomach meridians entered; has the effects of eliminating dampness, strengthening spleen, dispelling pathogenic wind and eliminating dampness. It can be used for treating damp obstruction of spleen and stomach, arthralgia due to wind-cold-dampness, and nyctalopia.
Rhizoma alismatis: cold in nature, sweet and bland in flavor; entering kidney and bladder meridians; has effects in promoting diuresis, eliminating dampness, and clearing away kidney fire; it can be used for treating edema, dysuria, diarrhea, and stranguria with turbid urine.
B, wood through: cold in nature, bitter in taste; heart, small intestine and bladder meridians entered; has effects of clearing heat, promoting diuresis, dredging channels and promoting lactation; it can be used for treating aphtha, tongue sore, bladder damp-heat, and galactostasis.
Dried orange peel: warm in nature, pungent and bitter in flavor; spleen and lung meridian entered; has effects of regulating qi-flowing, invigorating spleen, eliminating dampness and phlegm; it can be used for treating anorexia, abdominal distention, diarrhea, and cough due to damp phlegm.
Astragalus root: mild in nature and sweet in taste; spleen and lung meridian entered; has effects in invigorating qi, invigorating yang, consolidating superficial resistance, arresting sweating, removing toxic materials, promoting granulation, inducing diuresis, and relieving swelling; it is mainly used for treating spleen-lung qi deficiency, qi deficiency sinking, exterior deficiency sweating, sore and carbuncle, ulcer, qi deficiency edema.
Codonopsis pilosula: mild in nature and sweet in taste; spleen and lung meridian entered; has effects in invigorating middle warmer, invigorating qi, invigorating spleen, and nourishing lung; can be used for treating weakness of spleen and stomach, anorexia, diarrhea, cough with asthma due to lung deficiency, asthenia, and qi deficiency with prolapse.
White atractylodes rhizome: warm in nature, sweet and bitter in taste; spleen and stomach meridians entered; has effects of invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, consolidating superficial resistance, arresting sweating, invigorating qi, and preventing miscarriage; it can be used for treating diarrhea due to spleen deficiency, edema, spontaneous perspiration, and threatened abortion.
Tuckahoe, poria cocos: neutral in nature, sweet and bland in flavor, and entering spleen, stomach, heart, lung and kidney meridians; has effects in eliminating dampness, promoting diuresis, invigorating spleen, strengthening middle warmer, calming heart, and tranquilizing mind; it can be used for treating spleen and stomach weakness, phlegm dampness edema, and restlessness.
Licorice root: mild in nature and sweet in taste; entering twelve meridians; has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, moistening lung for arresting cough, and relieving drug property. Can be used for treating weakness of spleen and stomach, cough, sore, carbuncle, sore throat, and poisoning.
Lactobacillus plantarum: the microbial agent belongs to anaerobic bacteria, produces a large amount of organic acids such as acetic acid, propionic acid and the like and digestive enzymes in the metabolic process, can reduce the pH value of gastrointestinal tracts, is beneficial to activating and improving the activity of pepsin, and can effectively inhibit the growth and the propagation of pathogenic bacteria in an acidic environment; the lactein in the metabolite has the effect of inhibiting or killing escherichia coli, salmonella and the like; improving intestinal microenvironment, stimulating intestinal immune tissue development, and improving intestinal immune function.
Fermentation starter: belongs to facultative anaerobes, can produce a large amount of high-quality thalli, digestive enzyme, amino acid and vitamin in the metabolic process, and can supplement the nutrition of animals; rich enzymes can be generated in the fermentation process, the degradation of the cell walls of the traditional Chinese medicines of plants can be promoted, and the dissolution rate of the effective components is improved; regulating intestinal flora balance, stimulating the development of intestinal immune tissues, and improving intestinal immune function; improve the flavor of the fermentation product.
The Chinese pulsatilla root, the golden cypress, the golden thread and the ash bark in the formula of the invention clear away heat and toxic material, cool blood and stop dysentery; rhizoma atractylodis, rhizoma alismatis, akebia stem and poria cocos, astragalus mongholicus, codonopsis pilosula, rhizoma atractylodis macrocephalae and pericarpium citri reticulatae are used for eliminating dampness and promoting diuresis, tonifying qi, regulating qi and strengthening spleen, and liquorice is used for regulating the functions of the other drugs. The dissolution rate of the effective components in water extraction is improved by solid state fermentation of lactobacillus plantarum and yeast, and the effect of the traditional Chinese medicine extract is improved by the joint action of the fermented traditional Chinese medicine water extract and probiotics in clinical application through liquid state anaerobic fermentation of lactobacillus plantarum.
The usage of the traditional Chinese medicine microecological preparation for treating the diarrhea of the piglets comprises the following steps: the sick piglets within 7 days of age are orally taken for 2mL every day and are continuously taken for 2-3 days; the piglet from 7 days old to weaning attack is orally taken for 4mL every day and continuously taken for 2-3 days.
Preferably, the raw materials of the traditional Chinese medicine decoction pieces comprise the following components in parts by weight: 55-62 parts of Chinese pulsatilla root, 25-32 parts of golden cypress, 40-47 parts of coptis chinensis, 55-62 parts of ash bark, 10-17 parts of rhizoma atractylodis, 40-47 parts of rhizoma alismatis, 40-47 parts of akebia stem, 15-18 parts of dried orange peel, 55-58 parts of astragalus mongholicus, 25-28 parts of codonopsis pilosula, 25-28 parts of bighead atractylodes rhizome, 25-32 parts of poria cocos and 10-15 parts of liquorice.
More preferably, the raw materials of the traditional Chinese medicine decoction pieces comprise the following components in parts by weight: 60 parts of Chinese pulsatilla root, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 15 parts of rhizoma atractylodis, 45 parts of rhizoma alismatis, 45 parts of akebia stem, 20 parts of dried orange peel, 60 parts of astragalus mongholicus, 30 parts of codonopsis pilosula, 30 parts of bighead atractylodes rhizome, 30 parts of poria cocos and 15 parts of liquorice.
Preferably, in the first inoculation process, the inoculation amount is 1-1.2 times of the weight of the mixed traditional Chinese medicine micro powder, and more preferably 1.2 times.
Preferably, in the first inoculation process, the lactobacillus plantarum strain and the yeast strain have the same concentration and the volume ratio of 1: 2-2: 1, more preferably 1: 1.
preferably, in the first inoculation process, the viable count of the lactobacillus plantarum strain is (1.0-3.0) × 109CFU/mL, the viable count of the microzyme strain is (1.0-3.0) multiplied by 109CFU/mL. More preferably, the viable count of the Lactobacillus plantarum strain is 2.0X 109CFU/mL, viable count of the yeast strain is 2.0 × 109CFU/mL。
Preferably, in the secondary inoculation process, the inoculation amount is 5-10% of the weight of the traditional Chinese medicine extracting solution, and more preferably 10%.
Preferably, in the secondary inoculation process, the viable count of the lactobacillus plantarum strain is (5.0-10.0) × 108CFU/mL. More preferably, the viable count of the Lactobacillus plantarum strain is 5.0X 108CFU/mL。
In addition, the invention also provides a preparation method of the traditional Chinese medicine microecological preparation for treating piglet diarrhea. The preparation method comprises the following steps:
(1) crushing: crushing the raw materials of the traditional Chinese medicine decoction pieces into micro powder, sieving the micro powder by a sieve with 10-30 meshes, and mixing;
(2) primary inoculation: inoculating a mixture of lactobacillus plantarum strain and yeast strain;
(3) solid state fermentation: aerobic fermentation is carried out for 10-15 days at the temperature of 28-37 ℃, and the fermentation is stopped;
(4) water extraction: adding water with the weight 4-6 times that of the fermentation product, soaking for 6-8 hours, boiling for 2-3 hours, extracting for 2-3 times, and concentrating to 1-1.2 times of the weight of the traditional Chinese medicine micro powder mixture before solid state fermentation;
(5) and (3) sterilization: autoclaving at 115-121 ℃ for 40-60 min;
(6) secondary inoculation: when the temperature is reduced to 35-40 ℃, inoculating lactobacillus plantarum in an inoculation amount of 5-10% of the weight of the traditional Chinese medicine extracting solution;
(7) liquid state fermentation: carrying out anaerobic fermentation for 60-72 h at the temperature of 32-37 ℃, and stopping fermentation.
Preferably, in step (1), the powder is sieved through a 20-mesh sieve after being crushed.
Preferably, in step (3), the fermentation is terminated after 12 days of aerobic fermentation at 30 ℃.
Preferably, in the step (4), water with 5 times of the weight of the fermented product is added, the mixture is soaked for 8 hours, boiled for 3 hours, extracted for 2 times, and concentrated to 1 time of the weight of the traditional Chinese medicine micro powder mixture before solid state fermentation.
Preferably, in step (5), autoclaving is carried out at 121 ℃ for 40 min.
Preferably, in the step (6), after the temperature is reduced to 40 ℃, lactobacillus plantarum is inoculated according to the inoculation amount of 10 percent of the weight of the traditional Chinese medicine extracting solution;
preferably, in the step (7), anaerobic fermentation is carried out for 60h at the temperature of 37 ℃, and then fermentation is stopped and split charging is carried out.
The invention has the following beneficial effects:
(1) the traditional Chinese medicine microecological preparation disclosed by the invention is prepared from a plurality of traditional Chinese medicines with the effects of clearing away heat and toxic materials, strengthening spleen, eliminating dampness, cooling blood, stopping dysentery and the like in a proper proportion, so that the traditional Chinese medicine microecological preparation can effectively treat piglet diarrhea and improve the survival rate and the production performance of piglets;
(2) the traditional Chinese medicine microecological preparation contains lactobacillus plantarum, a large number of beneficial bacteria not only occupy the sites of harmful bacteria on the surface of intestinal mucosa of piglets, but also the lactobacillin secreted by the beneficial bacteria has the effect of inhibiting or killing escherichia coli, salmonella and the like; in addition, the organic acid generated by the beneficial bacteria can regulate the internal environment of the intestinal tract of the piglet into an acidic environment, so that the growth environment of harmful bacteria is lost, and the flora in the intestinal tract is kept relatively balanced; the comprehensive effects enhance the intestinal immune function of the piglets and greatly reduce the diarrhea rate;
(3) the traditional Chinese medicine microecologics provided by the invention adopts a solid-state and liquid-state double fermentation process, so that the action effect of the traditional Chinese medicine can be improved, the action function is expanded, active macromolecular substances in the traditional Chinese medicine are converted into small molecular substances which can be directly absorbed by intestinal tracts of piglets after fermentation, the digestion burden of the gastrointestinal tracts of the piglets is reduced, and the absorption rate of the piglets on the effective components of the traditional Chinese medicine is higher;
(4) the traditional Chinese medicine microecological preparation disclosed by the invention adopts a solid-state and liquid-state double fermentation process, acid and alcohol generated by beneficial bacteria have strong yeast fragrance, natural aromatic alcohol fragrance and acid fragrance, and animals have a very pleasant direction to the natural fragrance, so that after the traditional Chinese medicine microecological preparation is added into feed, a good food calling effect is achieved, the palatability of the feed can be improved, the food consumption of piglets is increased, and the production performance is further improved;
(5) the traditional Chinese medicine microecological preparation is administrated in an oral administration mode, so that the stress reaction of piglets is avoided;
(6) the traditional Chinese medicine microecological preparation is subjected to solid state fermentation by lactobacillus plantarum and saccharomycetes, so that the dissolution rate of the effective components of the traditional Chinese medicine in water extraction is improved, and the waste of traditional Chinese medicine decoction pieces is avoided.
Detailed Description
The present invention will be further described with reference to the following embodiments. The present invention is not limited to the following examples.
Example 1
The traditional Chinese medicine microecological preparation for treating piglet diarrhea is prepared by the following traditional Chinese medicine decoction pieces in parts by weight through crushing, inoculation, solid state fermentation, water extraction, sterilization and liquid state fermentation: 55 parts of Chinese pulsatilla root, 25 parts of golden cypress, 40 parts of coptis chinensis, 55 parts of ash bark, 10 parts of rhizoma atractylodis, 40 parts of rhizoma alismatis, 40 parts of akebia stem, 15 parts of dried orange peel, 55 parts of astragalus mongholicus, 25 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 25 parts of poria cocos and 10 parts of liquorice, crushing, sieving with a 10-mesh sieve, mixing, inoculating lactobacillus plantarum and yeast strains, wherein the inoculation amount is 1 time of the weight of the mixed traditional Chinese medicine powder, fermenting for 15 days at 28 ℃, and stopping fermentation; adding 6 times of water, soaking for 6 hr, boiling for 2 hr, extracting for 3 times, concentrating to 1 time of the weight of the Chinese medicinal materials before solid state fermentation, autoclaving at 115 deg.C for 40min, cooling to 35 deg.C, inoculating Lactobacillus plantarum in an amount of 10% of the weight of the extractive solution, performing anaerobic fermentation at 32 deg.C for 72 hr, terminating fermentation, and packaging.
The lactobacillus plantarum and the saccharomycete strain in the solid state fermentation process are a mixture of lactobacillus plantarum and saccharomycete strains, and the volume ratio of the lactobacillus plantarum to the saccharomycete strains is 1: 2.
the viable count of the lactobacillus plantarum strain is 3.0 multiplied by 109CFU/mL, viable count of the yeast strain is 3.0 × 109CFU/mL。
The viable count of the lactobacillus plantarum strain in the liquid fermentation process is 5.0 multiplied by 108CFU/mL。
The usage of the traditional Chinese medicine microecological preparation for treating piglet diarrhea in the embodiment is as follows: the sick piglets within 7 days of age are orally taken for 2mL every day and are continuously taken for 2-3 days; the piglet from 7 days old to weaning attack is orally taken for 4mL every day and continuously taken for 2-3 days.
Example 2
The traditional Chinese medicine microecological preparation for treating piglet diarrhea is prepared by the following traditional Chinese medicine decoction pieces in parts by weight through crushing, inoculation, solid state fermentation, water extraction, sterilization and liquid state fermentation: 65 parts of Chinese pulsatilla root, 35 parts of golden cypress, 50 parts of coptis chinensis, 65 parts of ash bark, 20 parts of rhizoma atractylodis, 50 parts of rhizoma alismatis, 50 parts of akebia stem, 25 parts of dried orange peel, 65 parts of astragalus mongholicus, 35 parts of codonopsis pilosula, 35 parts of bighead atractylodes rhizome, 35 parts of poria cocos and 20 parts of liquorice, crushing, sieving with a 30-mesh sieve, mixing, inoculating lactobacillus plantarum and yeast strains, wherein the inoculation amount is 1.2 times of the weight of the mixed traditional Chinese medicine powder, fermenting for 10 days at 37 ℃, and stopping fermentation; adding 4 times of water, soaking for 8 hr, boiling for 3 hr, extracting for 2 times, concentrating to 1.2 times of the weight of the Chinese medicinal materials before solid state fermentation, autoclaving at 121 deg.C for 60min, cooling to 40 deg.C, inoculating Lactobacillus plantarum in an amount of 5% of the weight of the extractive solution, performing anaerobic fermentation at 37 deg.C for 72 hr, terminating fermentation, and packaging.
The lactobacillus plantarum and the saccharomycete strain in the solid state fermentation process are a mixture of lactobacillus plantarum and saccharomycete strains, and the volume ratio of the lactobacillus plantarum to the saccharomycete strains is 2: 1.
the viable count of the lactobacillus plantarum strain is 1.0 multiplied by 109CFU/mL, saidThe viable count of the yeast strain is 1.0 × 109CFU/mL。
The viable count of the lactobacillus plantarum strain in the liquid fermentation process is 1.0 multiplied by 109CFU/mL。
The usage of the traditional Chinese medicine microecological preparation for treating piglet diarrhea in the embodiment is as follows: the sick piglets within 7 days of age are orally taken for 2mL every day and are continuously taken for 2-3 days; the piglet from 7 days old to weaning attack is orally taken for 4mL every day and continuously taken for 2-3 days.
Example 3
The traditional Chinese medicine microecological preparation for treating piglet diarrhea is prepared by the following traditional Chinese medicine decoction pieces in parts by weight through crushing, inoculation, solid state fermentation, water extraction, sterilization and liquid state fermentation: 60 parts of Chinese pulsatilla root, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 15 parts of rhizoma atractylodis, 45 parts of rhizoma alismatis, 45 parts of akebia stem, 20 parts of dried orange peel, 60 parts of astragalus membranaceus, 30 parts of codonopsis pilosula, 30 parts of bighead atractylodes rhizome, 30 parts of poria cocos and 15 parts of liquorice, crushing, sieving with a 20-mesh sieve, mixing, inoculating lactobacillus plantarum and yeast strains, wherein the inoculation amount is 1.2 times of the weight of the mixed traditional Chinese medicine powder, fermenting for 12 days at 30 ℃, and stopping fermentation; adding 5 times of water, soaking for 8 hr, boiling for 3 hr, extracting for 2 times, concentrating to 1 time of the weight of the Chinese medicinal materials before solid state fermentation, autoclaving at 121 deg.C for 40min, cooling to 40 deg.C, inoculating Lactobacillus plantarum in an amount of 10% of the weight of the extractive solution, performing anaerobic fermentation at 37 deg.C for 60 hr, terminating fermentation, and packaging.
The lactobacillus plantarum and the saccharomycete strain in the solid state fermentation process are a mixture of lactobacillus plantarum and saccharomycete strains, and the volume ratio of the lactobacillus plantarum to the saccharomycete strains is 1: 1.
the viable count of the lactobacillus plantarum strain is 2.0 multiplied by 109CFU/mL, viable count of the yeast strain is 2.0 × 109CFU/mL。
The viable count of the lactobacillus plantarum strain in the liquid fermentation process is 5.0 multiplied by 108CFU/mL。
The usage of the traditional Chinese medicine microecological preparation for treating piglet diarrhea in the embodiment is as follows: the sick piglets within 7 days of age are orally taken for 2mL every day and are continuously taken for 2-3 days; the piglet from 7 days old to weaning attack is orally taken for 4mL every day and continuously taken for 2-3 days.
Example 4
The traditional Chinese medicine microecological preparation for treating piglet diarrhea is prepared by the following traditional Chinese medicine decoction pieces in parts by weight through crushing, inoculation, solid state fermentation, water extraction, sterilization and liquid state fermentation: 58 parts of Chinese pulsatilla root, 28 parts of phellodendron bark, 43 parts of coptis root, 58 parts of ash bark, 13 parts of rhizoma atractylodis, 43 parts of rhizoma alismatis, 43 parts of akebiaquinata, 22 parts of dried orange peel, 62 parts of astragalus root, 32 parts of codonopsis pilosula, 32 parts of bighead atractylodes rhizome, 28 parts of poria cocos and 15 parts of liquorice, crushing, sieving with a 20-mesh sieve, mixing, inoculating lactobacillus plantarum and yeast strains, wherein the inoculation amount is 1.1 times of the weight of the mixed traditional Chinese medicine powder, fermenting for 15 days at 30 ℃, and stopping fermentation; adding 5 times of water, soaking for 8 hr, boiling for 3 hr, extracting for 2 times, concentrating to 1 time of the weight of the Chinese medicinal materials before solid state fermentation, autoclaving at 115 deg.C for 40min, cooling to 37 deg.C, inoculating Lactobacillus plantarum in an amount of 8% of the weight of the extractive solution, performing anaerobic fermentation at 32 deg.C for 60 hr, terminating fermentation, and packaging.
The lactobacillus plantarum and the saccharomycete strain in the solid state fermentation process are a mixture of lactobacillus plantarum and saccharomycete strains, and the volume ratio of the lactobacillus plantarum to the saccharomycete strains is 1: 1.5.
the viable count of the lactobacillus plantarum strain is 2.0 multiplied by 109CFU/mL, viable count of the yeast strain is 2.0 × 109CFU/mL。
The viable count of the lactobacillus plantarum strain in the liquid fermentation process is 1.0 multiplied by 109CFU/mL。
The usage of the traditional Chinese medicine microecological preparation for treating piglet diarrhea in the embodiment is as follows: the sick piglets within 7 days of age are orally taken for 2mL every day and are continuously taken for 2-3 days; the piglet from 7 days old to weaning attack is orally taken for 4mL every day and continuously taken for 2-3 days.
Example 5
The traditional Chinese medicine microecological preparation for treating piglet diarrhea is prepared by the following traditional Chinese medicine decoction pieces in parts by weight through crushing, inoculation, solid state fermentation, water extraction, sterilization and liquid state fermentation: 62 parts of Chinese pulsatilla root, 32 parts of golden cypress, 47 parts of coptis chinensis, 62 parts of ash bark, 17 parts of rhizoma atractylodis, 47 parts of rhizoma alismatis, 47 parts of akebia stem, 18 parts of dried orange peel, 58 parts of astragalus membranaceus, 28 parts of codonopsis pilosula, 28 parts of bighead atractylodes rhizome, 32 parts of poria cocos and 15 parts of liquorice, crushing, sieving with a 10-mesh sieve, mixing, inoculating lactobacillus plantarum and yeast strains, wherein the inoculation amount is 1.1 times of the weight of the mixed traditional Chinese medicine powder, fermenting for 12 days at 35 ℃, and stopping fermentation; adding 6 times of water, soaking for 6 hr, boiling for 2 hr, extracting for 3 times, concentrating to 1.2 times of the weight of the Chinese medicinal materials before solid state fermentation, autoclaving at 121 deg.C for 50min, cooling to 35 deg.C, inoculating Lactobacillus plantarum in an amount of 6% of the weight of the extractive solution, performing anaerobic fermentation at 35 deg.C for 60 hr, stopping fermentation, and packaging.
The lactobacillus plantarum and the saccharomycete strain in the solid state fermentation process are a mixture of lactobacillus plantarum and saccharomycete strains, and the volume ratio of the lactobacillus plantarum to the saccharomycete strains is 1.5: 1.
the viable count of the lactobacillus plantarum strain is 1.0 multiplied by 109CFU/mL, viable count of the yeast strain is 1.0 × 109CFU/mL。
The viable count of the lactobacillus plantarum strain in the liquid fermentation process is 5.0 multiplied by 108CFU/mL。
The usage of the traditional Chinese medicine microecological preparation for treating piglet diarrhea in the embodiment is as follows: the sick piglets within 7 days of age are orally taken for 2mL every day and are continuously taken for 2-3 days; the piglet from 7 days old to weaning attack is orally taken for 4mL every day and continuously taken for 2-3 days.
Comparative example 1
The traditional Chinese medicine preparation for treating piglet diarrhea in the comparative example is prepared by crushing, water extracting and sterilizing the following traditional Chinese medicine decoction pieces in parts by weight: 60 parts of Chinese pulsatilla root, 30 parts of golden cypress, 45 parts of coptis chinensis, 60 parts of ash bark, 15 parts of rhizoma atractylodis, 45 parts of rhizoma alismatis, 45 parts of akebia stem, 20 parts of dried orange peel, 60 parts of astragalus membranaceus, 30 parts of codonopsis pilosula, 30 parts of bighead atractylodes rhizome, 30 parts of poria cocos and 15 parts of liquorice, crushing, sieving with a 20-mesh sieve, mixing, adding 10 times of water by weight of traditional Chinese medicines, soaking for 8 hours, boiling for 3 hours, extracting for 2 times, concentrating to 1 time of the weight of the traditional Chinese medicines before solid state fermentation, sterilizing at 121 ℃ for 40min under.
Experimental example 1
The experimental example examines the treatment effect of the traditional Chinese medicine microecologics of examples 1 to 5 on piglet diarrhea.
(1) Test materials
Test drugs: examples 1-5 preparation of Chinese medicinal micro-ecological preparation
Test animals: 350 three-element fattening piglets of 2 days old are provided by a certain pig farm in the Pingting mountain city. The symptoms are diarrhea and the feces are yellow.
(2) Test method
The experiment adopts single-factor completely random design, and 7 groups are arranged in total, and each group has 50 piglets. The test groups 1 to 5 were drenched with the traditional Chinese medicine microecological preparation of the invention according to the dosage usage of the examples 1 to 5, the group 6 was a control group, and the traditional Chinese medicine preparation of the comparative example 1 was drenched, and the dosage usage was the same as that of the test groups 1 to 5. Group 7 was a blank group, no drug was fed, 3 days in the trial period, and the feeding environment was consistent for each group during the trial period. The treatment of each group of piglets at the end of the trial was recorded as shown in table 1 below.
TABLE 1 treatment of diarrhea in piglets by traditional Chinese medicine micro-ecological preparation
The cure rate of 1-5 sick piglets in the test group is more than 94 percent and the highest cure rate is 98 percent, and the feces of the piglets which are not cured are pasty, which shows that the preparation is effective and the effective rate is 100 percent; the control group has 78% of cure rate and 4% of death rate, 5 piglets are not cured but the excrement is pasty, 4 piglets are ineffective, and the effective rate is 88%; the mortality rate of the blank group is 12 percent, the self-healing rate reaches 56 percent at the end of the test, and the piglets are lean.
Experimental example 2
The experimental example examines the treatment effect of the traditional Chinese medicine microecological preparation of example 3 on piglet diarrhea.
(1) Test materials
Test drugs: example 3 preparation of Chinese medicine Microecological preparation
Test animals: 132 triple fattening pigs within 2 days of age are provided by a certain pig farm in the Zhou city. The symptoms are diarrhea and the disease is developed for 1 day.
(2) Test method
The inventive probiotic was drenched at the dose of example 3 for a test period of 3 days.
(3) Test results
After 48 hours of administration, more than 90% of the piglet feces are formed, 4 piglets do not have obvious improvement, and the rest is pasty; after 72h, 2 pigs die and 126 pigs are cured, and the rest of the piglet feces are pasty. The cure rate is 95.45 percent, the effective rate is 98.48 percent, and the treatment effect is obvious.
Experimental example 3
The experimental example examines the treatment effect of the traditional Chinese medicine microecological preparation of example 5 on piglet diarrhea.
(1) Test materials
Test drugs: example 5 preparation of Chinese medicine Microecological preparation
Test animals: 164 triple fattening pigs within 10 days of age were provided by a certain pig farm in Jingzhou city. The symptoms are diarrhea and the disease is developed for 1 day.
(2) Test method
The inventive probiotic was drenched at the dose of example 5 for a test period of 3 days.
(3) Test results
After the medicine is applied for 24 hours, more than 40 percent of piglet excrement is formed; after 48 hours, more than 90% of the piglet feces are formed, 5 piglets do not have obvious improvement, and the rest is pasty; after 72h, 157 piglets are cured, 3 piglets and 4 piglets are invalid, and the excrement is pasty and does not die. The cure rate is 95.73 percent, the effective rate is 98.17 percent, and the treatment effect is obvious.
Experimental example 4
The experimental example examines the treatment effect of the traditional Chinese medicine microecological preparation of example 2 on piglet diarrhea.
(1) Test materials
Test drugs: example 2 preparation of a Chinese medicinal Microecological preparation
Test animals: 152 triple fattening pigs within 15 days of age are provided by a certain pig farm in Kaifeng city. The symptoms are diarrhea and the disease is developed for 1 day.
(2) Test method
The inventive probiotic was drenched at the dose of example 2 for a test period of 3 days.
(3) Test results
After the medicine is applied for 24 hours, more than 70 percent of the piglet excrement is formed; after 48 hours, more than 90% of the piglet feces are molded, and the balance is pasty; after 72h, 148 piglets are cured, 3 piglets are pasty and die for 1 piglet. The cure rate is 97.37 percent, the effective rate is 99.34 percent, and the treatment effect is obvious.
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| CN113616715A (en) * | 2021-08-31 | 2021-11-09 | 昆明理工大学 | Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof |
| CN114271390A (en) * | 2021-12-23 | 2022-04-05 | 广东加大实业有限公司 | Plant feed for piglets and preparation method thereof |
| CN114271389A (en) * | 2021-12-23 | 2022-04-05 | 广东加大实业有限公司 | Weaned piglet feed and preparation method thereof |
| CN114366787A (en) * | 2021-12-09 | 2022-04-19 | 上海颐久生物科技有限公司 | Chinese herbal medicine microbial fermentation preparation and application methods thereof |
| CN114984114A (en) * | 2022-06-06 | 2022-09-02 | 湖北武当动物药业有限责任公司 | Liquid fermentation traditional Chinese medicine microecological preparation and application thereof in aspect of resisting porcine epidemic diarrhea virus |
| CN115462462A (en) * | 2022-09-23 | 2022-12-13 | 山东省黄泥湾农业科技有限公司 | Coarse cereal bran feed capable of treating piglet diarrhea and preparation method thereof |
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| CN113616715A (en) * | 2021-08-31 | 2021-11-09 | 昆明理工大学 | Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof |
| CN113616715B (en) * | 2021-08-31 | 2022-05-06 | 昆明理工大学 | Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof |
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| CN114271390A (en) * | 2021-12-23 | 2022-04-05 | 广东加大实业有限公司 | Plant feed for piglets and preparation method thereof |
| CN114271389A (en) * | 2021-12-23 | 2022-04-05 | 广东加大实业有限公司 | Weaned piglet feed and preparation method thereof |
| CN114984114A (en) * | 2022-06-06 | 2022-09-02 | 湖北武当动物药业有限责任公司 | Liquid fermentation traditional Chinese medicine microecological preparation and application thereof in aspect of resisting porcine epidemic diarrhea virus |
| CN114984114B (en) * | 2022-06-06 | 2023-12-29 | 湖北武当动物药业有限责任公司 | Liquid fermentation traditional Chinese medicine microecological preparation and application thereof in aspect of resisting porcine epidemic diarrhea virus |
| CN115462462A (en) * | 2022-09-23 | 2022-12-13 | 山东省黄泥湾农业科技有限公司 | Coarse cereal bran feed capable of treating piglet diarrhea and preparation method thereof |
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