CN112425681B - Application of bile acid composite microbial agent in preparation of pelteobagrus fulvidraco feed additive - Google Patents
Application of bile acid composite microbial agent in preparation of pelteobagrus fulvidraco feed additive Download PDFInfo
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- CN112425681B CN112425681B CN202011297926.3A CN202011297926A CN112425681B CN 112425681 B CN112425681 B CN 112425681B CN 202011297926 A CN202011297926 A CN 202011297926A CN 112425681 B CN112425681 B CN 112425681B
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- bile acid
- microbial inoculum
- pelteobagrus fulvidraco
- acid complex
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- 241000376029 Tachysurus fulvidraco Species 0.000 title claims abstract description 48
- 239000003613 bile acid Substances 0.000 title claims abstract description 48
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/575—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses an application of a bile acid complex microbial inoculum in preparation of a pelteobagrus fulvidraco feed additive. The bile acid complex microbial inoculum comprises a parabacteroides dieldii bacterial suspension, a bacteroides thetaiotaomicron bacterial suspension, bile acid and eucalyptus globulus oil, and the volume mass ratio of the bile acid complex microbial inoculum is 2-3. The Bacteroides gasseri strain suspension is prepared from Bacteroides gasseri LCG-06 with the preservation number of CGMCC No.20820, and the Bacteroides thetaiotaomicron strain suspension is prepared from Bacteroides thetaiotaomicron GL-02 with the preservation number of CGMCC No.20818. The compound microbial inoculum disclosed by the invention is natural in components and free from toxic and side effects, can remarkably improve and restore intestinal health of the pelteobagrus fulvidraco, further protects the liver and protects the gallbladder and repairs liver and gallbladder injury, has the effects of resisting bacteria, diminishing inflammation and expelling parasites, enhances the physique and anti-stress capability of the pelteobagrus fulvidraco, and improves the breeding income of the pelteobagrus fulvidraco, so that the compound microbial inoculum has a wide application prospect.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to an application of a bile acid complex microbial inoculum in preparation of a pelteobagrus fulvidraco feed additive.
Technical Field
The yellow catfish is a scaleless fish, belongs to a small-sized fresh water famous and excellent aquaculture variety, is rich in amino acid, tender in meat quality, delicious in taste and high in nutritional value, has nourishing effect and medicinal value, and is deeply loved by people. In the current pelteobagrus fulvidraco breeding mode, the pelteobagrus fulvidraco is quick, high and rich in pursuit, diseases such as gastroenteritis, hepatobiliary syndrome and the like are easily caused to fish groups due to excessive feeding and excessively dense living environment, and the current novel pelteobagrus fulvidraco's head splitting disease' can be caused. When the fish school is diseased, the treatment needs to consume time, medicines, manpower and money, the breeding consumption and cost are increased, the yield is reduced, the breeding period is prolonged, the breeding cost is increased, and the economic loss of the breeding is caused.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides an application of a bile acid complex microbial inoculum in preparation of a pelteobagrus fulvidraco feed additive. The bile acid complex microbial inoculum can protect the liver and the gallbladder and repair the damage of the liver and the gallbladder of the yellow catfish, and can effectively kill insects and inhibit bacteria.
In order to realize the purpose of the invention, the invention adopts the following technical scheme to realize:
the invention relates to an application of a bile acid complex microbial inoculum in preparation of a pelteobagrus fulvidraco feed additive.
Further, the bile acid complex microbial inoculum comprises a bacteroides deltoidea suspension, a bacteroides thetaiotaomicron suspension, bile acid and eucalyptus globulus essential oil.
Further, the volume mass ratio of the parabacteroides dycolchicum suspension to the bacteroides thetaiotaomicron suspension to the bile acid to the eucalyptus globulus essential oil is 2-3.
Further, the paradisella dirichiana bacterial suspension is prepared from paradisella dirichiana LCG-06 with the preservation number of CGMCC No.20820.
Further, the preparation method of the paradisella dieselii LCG-06 bacterial suspension comprises the following steps: activating the paradisella dieldii LCG-06 in a nutrient liquid culture medium for 48 hours at 37 ℃ in an anaerobic environment; adding a sucrose solution with the mass concentration of 20% into the activated bacterium liquid, uniformly mixing, centrifuging, adding a sucrose solution with the mass concentration of 10% into the precipitate, and re-suspending to obtain the paradise dychii LCG-06 bacterium suspension.
Further, the bacterial content of the paradisella dieselii LCG-06 bacterial suspension is not less than 2 multiplied by 10 9 CFU/mL。
Further, the bacteroides thetaiotaomicron bacterial suspension is prepared from bacteroides thetaiotaomicron GL-02 with the preservation number of CGMCC No.20818.
Further, the total bacteria content of the bile acid complex microbial inoculum is not less than 6 multiplied by 10 8 CFU/mL。
Further, the use method of the bile acid complex microbial inoculum comprises the following steps: adding the bile acid complex microbial inoculum into the culture feed of the pelteobagrus fulvidraco, feeding for 7-15 days 3 times per day.
Further, the addition amount of the bile acid complex microbial inoculum is 1-3mL/kg of feed.
Further, the bile acid complex microbial inoculum can restore the intestinal health of the yellow catfish and repair the liver and gall injury.
Further, the bile acid compound microbial inoculum can enhance the physique and the anti-stress capability of the pelteobagrus fulvidraco.
Compared with the prior art, the invention has the following advantages and beneficial effects:
according to the invention, one strain of parabacteroides diesei LCG-06 and one strain of bacteroides thetaiotaomicron GL-02 are respectively screened from pig manure, and do not cause harm to animal human bodies, the two strains are fermented to prepare a bacterial suspension, and the bacterial suspension, bile acid and eucalyptus globulus oil are jointly prepared to obtain the composite microbial inoculum.
Drawings
FIG. 1 is a photograph of the colony of Parabacteroides diesei LCG-06.
FIG. 2 is a photograph of colony of Bacteroides thetaiotaomicron GL-02.
Fig. 3 is a pelteobagrus fulvidraco with problems in the breeding process in example 2; the upper figure is pelteobagrus fulvidraco with abdominal cavity hydrops, and the lower figure is the viscera of pelteobagrus fulvidraco with liver and gall syndrome and bacterial infection after dissection.
Fig. 4 shows that the normal pelteobagrus fulvidraco liver is recovered after the bile acid complex microbial inoculum is used.
Fig. 5 is the pelteobagrus fulvidraco diagnosed with head cracking disease in example 3.
Fig. 6 is pelteobagrus fulvidraco without obvious damage using the bile acid complex microbial inoculum.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific examples. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers.
Example 1:
1. screening and identification of Parabacteroides dieldii LCG-06
1. Screening of Parabacteroides dycolchica LCG-06
Taking intestinal feces from pig intestinal tract, mixing 10g feces with 100mL sterile water, filtering to remove impurities to obtain feces diluent, and adding sterile water to the diluent to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Diluting the solution; respectively take 10 -2 、10 -3 、10 -4 、10 -5 The diluted solution was applied to soybean casein agar medium (TSA) at a content of 80% by volume N 2 、10％CO 2 And 10% of H 2 Culturing at 37 deg.C for 4 days in anaerobic environment, and selecting obvious bacterial colonyAfter multiple purifications, a single colony is obtained and named as LCG-06, and then placed in a soybean casein agar slant culture medium for cold storage. The formula of the TSA culture medium is as follows:
tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, agar 15.0g, sterile water 1000mL, pH 7.3 + -0.2.
As shown in FIG. 1, the colony biological characteristics of the strain LCG-06 in the TSA medium are: round or round-like, milky white or off-white, flat, 0.2-1.8mm in diameter, smooth and lusterless surface, semitransparent and relatively neat edge.
2. Identification of Parabacteroides dieldii LCG-06
Extracting genome DNA of the strain LCG-06, taking the genome DNA as a template, performing PCR amplification by using a 16S rRNA universal primer to obtain a 16S rRNA amplification sequence, sequencing, and performing Blast comparison on the sequence to show that the homology of the strain LCG-06 and paradises disactinatis the highest in a GenBank gene library, so that the strain LCG-06 is judged to be paradise dibromica.
And (3) performing strain preservation on the screened paradisella dirichiana LCG-06, wherein the preservation unit comprises: china general microbiological culture Collection center (CGMCC); address: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: 09 month and 25 days 2020; the preservation number of the Parabacteroides dibacteroides distasonis is CGMCC No.20820.
3. Preparation of a suspension of Parabacteroides dycolchicus LCG-06 bacteria
(1) Selecting the preserved paradise dirichi LCG-06 into a nutrient liquid culture medium (the formula is tryptone 15.0g, soyapeptone 5.0g, sodium chloride 5.0g, sterile water 1000mL, pH 7.4), and performing activated culture for 48h at 37 ℃ in an anaerobic environment to obtain paradise dirichi LCG-06 bacterial liquid;
(2) Adding a sucrose solution with the mass concentration of 20% into the bacterial liquid according to the volume ratio of 1;
(3) Re-dissolving the precipitate of the above strain with 10% by mass of sucrose solution, the volume of which isAnd mixing with the bacterial strain precipitate according to the mass ratio of 2 9 CFU/mL。
2. Screening and identification of Bacteroides thetaiotaomicron GL-02
1. Screening of Bacteroides thetaiotaomicron GL-02
Mixing 10g of healthy pig feces with 100mL of sterile water, filtering to remove impurities to obtain feces diluent, and adding sterile water into the diluent to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Diluting the solution; respectively taking 10 -2 、10 -3 、10 -4 、10 -5 The diluted solution was applied to GAM agar medium and the content was 80% N 2 、10％CO 2 And 10% of H 2 Culturing at 35 ℃ for 48h in an anaerobic environment, selecting obvious bacterial colonies, purifying for multiple times to obtain single bacterial colonies, naming the obtained single bacterial colonies as GL-02, and freezing and preserving. Wherein the GAM agar culture medium comprises the following components: 10.0g peptone, 3.0g soybean peptone, 10.0g monthly peptone, 13.5g serum digest powder, 2.2g beef extract powder, 5.0g yeast extract powder, 1.2g beef liver extract powder, 3.0g glucose, 3.0g sodium chloride, 2.5g potassium dihydrogen phosphate, 5.0g soluble starch, 0.3g L-cysteine, 0.15g sodium thioglycolate, 15.0g agar, 1mL of 0.1% vitamin K1 solution, 3mL hemin chloride solution, 100mg kanamycin, 1000mL distilled water, pH 7.1. As shown in FIG. 2, the colony of the strain GL-02 on GAM agar medium is round or round-like, yellow, 0.5-2.5mm in diameter, smooth and lusterless in surface, slightly convex in middle, opaque, and relatively neat in edge.
2. Identification of Bacteroides thetaiotaomicron GL-02
Extracting genome DNA of the strain GL-02, using the genome DNA as a template, performing PCR amplification by using a 16S rRNA universal primer to obtain a 16S rRNA amplification sequence, sequencing, and performing Blast comparison on the sequence to show that the strain GL-02 has the highest homology with Bacteroides thetaiotaomicron in a GenBank gene library, thereby judging the strain GL-02 to be Bacteroides thetaiotaomicron.
And (3) performing strain preservation on the screened Bacteroides thetaiotaomicron GL-02, wherein the preservation unit comprises the following steps: china general microbiological culture Collection center (CGMCC); address: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: 09 month and 25 days 2020; the preservation number of the Bacteroides thetaiotaomicron is CGMCC No.20818.
3. Preparation of Bacteroides thetaiotaomicron GL-02 bacterial suspension
Selecting the preserved Bacteroides thetaiotaomicron GL-02 to GAM liquid culture medium (the agar can be reduced on the formula of the GAM agar culture medium), activating for 48h under 35 ℃ anaerobic environment, inoculating 1mL of the obtained activated bacterium liquid into 50mL of GAM liquid culture medium again for culturing for 24-76h, centrifuging the culture solution at 10000rpm/min for 5min, then re-suspending the strain precipitate with sterile physiological saline with the volume 10-12 times of the mass of the strain precipitate, centrifuging at 10000rpm/min for 5min again, re-suspending with sterile physiological saline with the volume 5 times of the mass of the strain precipitate, and ensuring that the bacterium content of the Bacteroides thetaiotaomicron GL-02 in the heavy suspension is not less than 1 x 10 9 CFU/mL。
3. Preparation of bile acids
The preparation process of the bile acid comprises the following steps:
(1) Saponification: adding 1kg of pig gall powder crushed and sieved by a 40-mesh sieve into a reaction kettle, adding 10L of sodium hydroxide solution with the mass concentration of 10%, heating and stirring until the mixture is boiled, keeping the boiling state and stirring for 14 hours, then cooling saponification liquid generated by the reaction until solid and liquid are layered, removing supernatant, and obtaining the residual solid which is bile acid saponification product;
(2) And (3) decoloring: adding 6L of water into the bile acid saponified product, heating to 80 ℃ to completely dissolve the bile acid saponified product, then pumping the bile acid saponified product into a decoloring tank, adding 300mL of hydrogen peroxide, uniformly stirring, reacting at normal temperature for 24 hours, and then filtering the reaction solution into an acidification tank to obtain a filtrate;
(3) Acidifying: cooling the filtrate to room temperature, slowly adding 10% hydrochloric acid solution while stirring, stopping adding acid when the pH value of the solution is 3, and centrifugally filtering to obtain a white solid;
(4) And (3) purification: continuously adding water to wash the white solid matter, removing water-soluble impurities attached to the surface of the white solid matter, and finishing the water washing after the filtered water is detected to be neutral; placing the white solid after centrifugal filtration into a drying oven, and drying at 100 ℃ until the moisture is less than 10% to obtain a bile acid crude product;
(5) And (3) recrystallization: putting the bile acid crude product into an extraction tank, adding ethyl acetate with the volume 10 times of the weight of the bile acid crude product, stirring until the bile acid crude product is completely dissolved, then adding 0.08g of anhydrous sodium sulfate for dehydration, filtering and collecting filtrate, carrying out reduced pressure concentration on the filtrate, and recovering ethyl acetate to obtain liquid bile acid;
(6) And (3) drying: and (3) putting the liquid bile acid into an oven, and drying at 100 ℃ until the water content is not more than 1% to obtain the finished product bile acid.
The finished bile acid contains 78.6% of hyocholic acid and hyodeoxycholic acid and 20.0% of chenodeoxycholic acid.
4. Preparation method of eucalyptus globulus oil
Washing Australian multi-bud eucalyptus leaves in a washing tower, putting the Australian multi-bud eucalyptus leaves in a dryer for drying, crushing the Australian multi-bud eucalyptus leaves in a crusher, then respectively putting the crushed Australian multi-bud eucalyptus leaves into a steam distillation kettle, a condenser and an oil-water delayer for separating an oil phase and a water phase, putting the water phase into an ether extractor for separating wastewater, putting liquid containing ether into an ether recovery tower for recovering the ether, adding the recovered ether and oil phase liquid into a solid bed adsorption dryer for further removing water in the oil phase, and finally adding the oil phase liquid into a reduced pressure distillation tower for rectification so as to remove impurity oil, thereby obtaining the multi-bud eucalyptus essential oil. The prepared eucalyptus globulus labill essential oil is stored in a sealed and lightproof container and is placed away from light.
5. Preparation of bile acid composite bacterial agent
Uniformly mixing the prepared bile acid, eucalyptus globulus essential oil, bacteroides thetaiotaomicron GL-02 bacterial suspension and bacteroides delbrueckii LCG-06 bacterial suspension according to the mass volume ratio of 2; the total bacteria content in the composite microbial inoculum is not less than 6 multiplied by 10 8 CFU/mL。
Example 2
70 mu of pelteobagrus fulvidraco is cultured in a Zhejiang lake pond, as shown in figure 3, a lot of fishes with large and swollen bellies appear in the culture, a large amount of death occurs, and 70 jin of dead fishes need to be cleaned by farmers every day. But the anatomical diagnosis of veterinarians shows that besides the yellow catfish infects the spider beetles, the liver also has serious hepatobiliary syndrome to cause necrosis, and the two problems cause the death of the belly of the yellow catfish after the abdominal cavity of the yellow catfish is ponded due to the secondary infection of bacteria.
The compound microbial inoculum disclosed by the invention is mixed into a feed for feeding pelteobagrus fulvidraco according to the proportion of 2mL/kg of feed, the pelteobagrus fulvidraco is fed for 3 times every day, after the pelteobagrus fulvidraco is fed for 1 week, the death number of the pelteobagrus fulvidraco in a pond is reduced, after the pelteobagrus fulvidraco is fed for 2 weeks, no fish with big belly exists in the pond, the liver color of the fish after dissection is ruddy and elastic, and the intestinal canal is full and has good toughness (figure 4), so that the compound microbial inoculum disclosed by the invention can restore the intestinal canal health of the pelteobagrus fulvidraco, further protect the liver and gallbladder, repair the liver and gallbladder injury of the pelteobagrus fulvidraco, and simultaneously has the effects of resisting bacteria, diminishing inflammation and expelling parasites.
Example 3
30 mu of pelteobagrus fulvidraco is cultured in a certain fish pond in Jiangsu, 2 mu of the compound microbial inoculum is randomly selected to be added into the special feed for the pelteobagrus fulvidraco, the addition amount is 1.5L/t of the feed, and all the pelteobagrus fulvidraco are cultured according to daily culture. In the process of breeding, hyperemia at the top of the head of the yellow catfish without using the compound microbial inoculum is found, the skin of the head is festered, the top of the head of the yellow catfish is perforated seriously and cracked, even the skull is caved, a long and narrow cavity is formed, brain tissues are exposed (figure 5), and the yellow catfish is diagnosed as head cracking disease. However, in the same breeding time, the pelteobagrus fulvidraco using the compound microbial inoculum only has slight edema on the back and has no obvious damage (figure 6), which shows that the compound microbial inoculum of the invention can enhance the physique and the anti-stress capability of the pelteobagrus fulvidraco, also has good bacteriostatic effect, reduces the possibility of the pelteobagrus fulvidraco being ill, and has the advantages of simple preparation and use, good effect, short treatment course, no side effect on the pelteobagrus fulvidraco, safety and reliability.
The above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (7)
1. An application of a bile acid complex microbial inoculum in preparation of a pelteobagrus fulvidraco feed additive is characterized in that the bile acid complex microbial inoculum comprises a parabacteroides dieldii bacterial suspension, a bacteroides thetaiotaomicron bacterial suspension, bile acid and eucalyptus globulus oil; the bacteroides thetaiotaomicron suspension is prepared from bacteroides thetaiotaomicron GL-02 with the preservation number of CGMCC No. 20818;
the volume-mass ratio of the parabacteroides delbrueckii bacterial suspension to the bacteroides thetaiotaomicron bacterial suspension to the bile acid to the eucalyptus globulus essential oil is (2-3).
2. The use according to claim 1, wherein the paradisella dieldii bacterial suspension is prepared from paradisella dieldii LCG-06 with the collection number CGMCC No.20820.
3. The use of claim 1, wherein the total bacterial content of the bile acid complex microbial inoculum is not less than 6 x 10 8 CFU/mL。
4. The use of claim 1, wherein the bile acid complex microbial inoculum is used by the method comprising the following steps: adding the bile acid complex microbial inoculum into the culture feed of the pelteobagrus fulvidraco, feeding for 7-15 days 3 times per day.
5. The use of claim 4, wherein the bile acid complex microbial inoculum is added in an amount of 1-3mL/kg feed.
6. The use of claim 1, wherein the bile acid complex microbial inoculum can restore intestinal health and repair liver and gall injury of the yellow catfish.
7. The application of claim 1, wherein the bile acid complex microbial inoculum can enhance the physique and the anti-stress capability of the pelteobagrus fulvidraco.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157595A (en) * | 2017-12-27 | 2018-06-15 | 郑州神草生物技术有限公司 | A kind of probiotics and preparation method thereof |
| CN108522858A (en) * | 2018-07-09 | 2018-09-14 | 山东龙昌动物保健品有限公司 | Application of the bile acid in preparing the feed addictive for Micropterus salmoides |
| CN109527273A (en) * | 2019-01-15 | 2019-03-29 | 无锡华诺威动物保健品有限公司 | A kind of feed addictive and preparation method for protecting special aquatic products fish liver health |
| CN110172427A (en) * | 2019-06-11 | 2019-08-27 | 江南大学 | A kind of method and application improving Di Shi pair bacteroid freeze-drying survival rate |
| CN110537504A (en) * | 2019-09-20 | 2019-12-06 | 山东龙昌动物保健品有限公司 | Eucalyptus multicastus essential oil and application of compound microbial inoculum thereof in preparation of insect repellent for marine fishes |
-
2020
- 2020-11-19 CN CN202011297926.3A patent/CN112425681B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157595A (en) * | 2017-12-27 | 2018-06-15 | 郑州神草生物技术有限公司 | A kind of probiotics and preparation method thereof |
| CN108522858A (en) * | 2018-07-09 | 2018-09-14 | 山东龙昌动物保健品有限公司 | Application of the bile acid in preparing the feed addictive for Micropterus salmoides |
| CN109527273A (en) * | 2019-01-15 | 2019-03-29 | 无锡华诺威动物保健品有限公司 | A kind of feed addictive and preparation method for protecting special aquatic products fish liver health |
| CN110172427A (en) * | 2019-06-11 | 2019-08-27 | 江南大学 | A kind of method and application improving Di Shi pair bacteroid freeze-drying survival rate |
| CN110537504A (en) * | 2019-09-20 | 2019-12-06 | 山东龙昌动物保健品有限公司 | Eucalyptus multicastus essential oil and application of compound microbial inoculum thereof in preparation of insect repellent for marine fishes |
Non-Patent Citations (1)
| Title |
|---|
| 拟杆菌微生态制剂对仔猪免疫能力的影响;曾红根等;《中国微生态学杂志》;20111231;第23卷(第11期);第989-990页 * |
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Address after: No. 9 Yanying North Road, Yanbei Street Office, Qihe County, Dezhou City, Shandong Province, 253000 Patentee after: Shandong Longchang Animal Health Products Co.,Ltd. Address before: 251100 No.1, North Park Road, Qihe Economic Development Zone, Dezhou City, Shandong Province Patentee before: SHANDONG LONGCHANG ANIMAL HEALTH PRODUCT Co.,Ltd. |