CN112415187B - A homogeneous immunoassay analyzer - Google Patents
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- CN112415187B CN112415187B CN202011356209.3A CN202011356209A CN112415187B CN 112415187 B CN112415187 B CN 112415187B CN 202011356209 A CN202011356209 A CN 202011356209A CN 112415187 B CN112415187 B CN 112415187B
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- 238000003018 immunoassay Methods 0.000 title claims description 28
- 239000000523 sample Substances 0.000 claims abstract description 256
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 165
- 238000006243 chemical reaction Methods 0.000 claims abstract description 112
- 238000001514 detection method Methods 0.000 claims abstract description 98
- 238000011534 incubation Methods 0.000 claims abstract description 74
- 238000011084 recovery Methods 0.000 claims description 19
- 239000012723 sample buffer Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 238000004064 recycling Methods 0.000 claims description 6
- 210000001503 joint Anatomy 0.000 claims description 3
- 239000007790 solid phase Substances 0.000 abstract description 6
- 230000036039 immunity Effects 0.000 abstract description 2
- 239000012071 phase Substances 0.000 abstract description 2
- 230000003287 optical effect Effects 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 12
- 239000000463 material Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 239000002699 waste material Substances 0.000 description 4
- 238000007885 magnetic separation Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010339 medical test Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002910 solid waste Substances 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000012611 container material Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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Abstract
The invention discloses a homogeneous phase immunity analyzer, comprising: an operation table; the sample storage and conveying module, the reaction container storage and loading module, the reagent storage and conveying module, the incubation module and the detection module are arranged above the operation table; the sample storage and conveying module is located at one end of the operating platform, the reagent storage and conveying module is located at the other end of the operating platform, the incubation module is located at the middle of the operating platform, and the reaction container storage and loading module and the detection module are both arranged beside the incubation module. According to the invention, a solid-phase carrier and a solid-phase probe are not needed, the antigen-antibody complex and the free antigen-antibody are not needed to be separated, the sample to be detected can be directly measured, and meanwhile, the invention has the advantages of compact structure, simple and quick operation, high automation degree and wide market application value.
Description
Technical Field
The present invention relates to the field of medical testing. More particularly, the present invention relates to a homogeneous immunoassay analyzer.
Background
In the field of medical testing, it is known to use analysis devices of different configurations for carrying out detection analysis of a sample. In the process of researching and realizing detection analysis of samples, the inventor finds that the analysis device in the prior art has at least the following problems:
Most of the existing analysis devices adopt chemiluminescent immunoassay technology, and have complex structure, complex technology, complex operation and easy error, for example: the main flow technology adopted by the chemiluminescence immunoassay technology is magnetic particle chemiluminescence, and the core assumption is that the separation of a specific immune complex and a biological matrix can be realized by magnetic separation, but in clinical practical application, cases of magnetic separation failure, such as the phenomenon of 'jump value' caused by non-specific adsorption caused by fibrin adhesion and false positive caused by heterophilic antibodies, are unavoidable due to the diversity of clinical specimens, meanwhile, the magnetic separation failure can be caused due to the easy occurrence of magnetic cleaning failure in the cleaning process of an analysis device, so that the analysis device cannot accurately analyze, the analysis and detection cost is higher, the analysis and detection technology cannot be widely applied to the instant detection fields of emergency treatment and the like, and meanwhile, the conventional instant detection type immunoassay device has common problems of poor precision, such as a colloidal gold immunoassay device, a fluorescent chromatography immunoassay device, a colloidal turbidimetric immunoassay device and the like, and can only be used as an auxiliary primary screening detection means, and finally, the chemiluminescence immunoassay is required to be confirmed.
And after the antigen-antibody characteristic reaction is finished, the homogeneous fluorescent immunoassay can be directly used for measurement without separating an antigen-antibody complex from free antigen-antibody.
In view of the above, there is a need to develop a homogeneous immunoassay analyzer for solving the above problems.
Disclosure of Invention
In view of the shortcomings in the prior art, the main purpose of the invention is to provide a homogeneous immunoassay analyzer, which loads a reaction container to an incubation module through a reaction container storage and loading module, loads a sample to be detected into the reaction container positioned on the incubation module through a sample storage and conveying module, conveys a detection reagent into the reaction container positioned on the incubation module through a reagent storage and conveying module, so that the detection reagent is mixed with the sample to be detected, and measures and detects the mixed liquid in the reaction container through the detection module; the homogeneous immunoassay analyzer disclosed by the invention does not need a solid-phase carrier and a solid-phase probe, does not need to separate an antigen-antibody complex from free antigen-antibody, can directly measure a sample to be detected, and has the advantages of compact structure, simplicity and rapidness in operation, high automation degree and wide market application value.
The invention further aims to provide a homogeneous immunoassay analyzer which is provided with an emergency loading unit, a user can place a sample to be detected which needs emergency treatment to the emergency loading unit, the sample to be detected is conveyed to an emergency loading station of the sample conveying line by the emergency loading unit, and the sample to be detected which needs emergency treatment is prevented from being placed in a first sample buffer area to be conveyed for detection in a queuing manner through the sample conveying line, so that the working efficiency is improved, the working time is saved, and the instant immunoassay analyzer is suitable for instant detection of the existing emergency treatment and the like.
To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a homogeneous immunoassay analyzer comprising: an operation table; and
The sample storage and conveying module, the reaction container storage and loading module, the reagent storage and conveying module, the incubation module and the detection module are arranged above the operation table;
The sample storage and conveying module is positioned at one end of the operating platform, the reagent storage and conveying module is positioned at the other end of the operating platform, the incubation module is positioned in the middle of the operating platform, and the reaction container storage and loading module and the detection module are both arranged beside the incubation module;
the reaction container storage loading module loads the reaction container to the incubation module, the sample storage conveying module conveys the sample to be detected to the reaction container located on the incubation module, the reagent storage conveying module conveys the detection reagent to the reaction container located on the incubation module, the detection reagent is mixed with the sample to be detected, and the detection module measures and detects the mixed liquid in the reaction container.
Preferably, the sample storage and delivery module comprises: the sample conveying line is sequentially provided with an emergency treatment feeding station, a sample identification station, a sample loading station and a sample recovery station along the conveying direction of the sample conveying line;
The sample conveying unit is arranged beside the sample conveying line and is positioned at a sample feeding station;
the sample identification module is arranged beside the sample conveying line and is positioned at a sample identification station; and
The sample recovery unit is arranged beside the sample conveying line and is positioned at a sample recovery station;
the sample conveying unit is used for conveying the sample to be detected to the sample feeding station, the sample identifying module is used for identifying the sample to be detected, and the sample recycling unit is used for recycling the sample.
Preferably, the sample storage and delivery module further comprises: the emergency loading unit is positioned at the emergency feeding station; and
And the sample adding unit is positioned at the sample adding station and used for conveying samples positioned at the sample adding station into the reaction container on the incubation module.
Preferably, the reaction vessel storage loading module includes: a reaction vessel storage tray on which at least two reaction vessels are stored; and
The movable grabbing module is arranged at the side of the reaction container storage disc;
the movable grabbing module grabs and places the reaction containers stored on the reaction container storage disc on the incubation module in sequence.
Preferably, the reagent storage and delivery module comprises: the reagent rotary table is provided with at least two reagent containers above, wherein detection reagents are stored in the reagent containers, and the reagent rotary table is sequentially provided with a reagent recognition station and a reagent sample adding station along the rotation direction of the reagent rotary table;
The reagent identification module is arranged beside the reagent turntable and is positioned at the reagent identification station; and
The reagent sample adding module is arranged beside the reagent turntable and is positioned at the reagent sample adding station;
The reagent identification module is used for identifying the reagent container positioned at the reagent identification station so as to identify the detection reagent in the reagent container, and the reagent sample adding module is used for loading the detection reagent in the reagent container positioned at the reagent sample adding station into the reaction container.
Preferably, the incubation module is an incubation turntable, at least two placement parts are arranged above the incubation turntable, each placement part is regularly arrayed along the periphery of the incubation turntable, and the placement parts are used for placing reaction containers;
The rotary table is provided with a reaction container feeding station, a sample feeding station, a reagent feeding station, an incubation station and a detection station along the rotation direction of the rotary table;
The reaction container storage loading module is located the reaction container material loading station, the sample application unit is located the sample material loading station, the reagent application module is located the reagent material loading station, the detection module is located the detection station.
Preferably, a temperature control module is arranged below the incubation module, and the temperature control module accurately controls the incubation temperature of the incubation module.
Preferably, the detection module includes: the device comprises a photon counting unit, an optical filter assembly, a dichroic mirror, a light source and a light source optical filter;
the two-direction mirror is obliquely arranged, the two-direction mirror is positioned right above the detection station, the light source is arranged beside the two-direction mirror, the light source optical filter is arranged between the light source and the two-direction mirror, the photon counting unit and the optical filter component are both arranged above the two-direction mirror, and the optical filter component is arranged between the photon counting unit and the two-direction mirror;
The light source provides a detection light source, the filter component and the light source filter are used for selecting light with specific wavelength, and the photon counting unit collects final photon signals.
One of the above technical solutions has the following advantages or beneficial effects: loading the reaction container to the incubation module through the reaction container storage loading module, loading the sample to be detected into the reaction container positioned on the incubation module through the sample storage conveying module, conveying the detection reagent into the reaction container positioned on the incubation module through the reagent storage conveying module, so that the detection reagent is mixed with the sample to be detected, and measuring and detecting the mixed liquid in the reaction container through the detection module; the homogeneous immunoassay analyzer disclosed by the invention does not need a solid-phase carrier and a solid-phase probe, does not need to separate an antigen-antibody complex from free antigen-antibody, can directly measure a sample to be detected, and has the advantages of compact structure, simplicity and rapidness in operation, high automation degree and wide market application value;
The other technical scheme has the following advantages or beneficial effects: the emergency loading unit is arranged, a user can place a sample to be detected which needs emergency treatment into the emergency loading unit, the sample to be detected is conveyed to an emergency loading station of the sample conveying line by the emergency loading unit, and the sample to be detected which needs emergency treatment is prevented from being placed into a first sample buffer area to be conveyed for detection in a queuing manner through the sample conveying line, so that the working efficiency is improved, the working time is saved, and the emergency loading unit is suitable for instant detection of the existing emergency and the like.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
For a clearer description of an embodiment of the invention, reference will be made to the accompanying drawings of embodiments, which relate to some embodiments of the invention only, and not to limitations of the invention, wherein:
FIG. 1 is a schematic diagram of a homogeneous immunoassay analyzer according to an embodiment of the present invention;
fig. 2 is a schematic diagram of an optical path of a detection module according to an embodiment of the invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other examples, which a person of ordinary skill in the art would obtain without undue burden based on the embodiments of the invention, are within the scope of the invention.
In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components.
Unless defined otherwise, technical or scientific terms used herein should be given the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The terms "first," "second," and the like in the description and in the claims, are not used for any order, quantity, or importance, but are used for distinguishing between different elements. Likewise, the terms "a," "an," or "the" and similar terms do not denote a limitation of quantity, but rather denote the presence of at least one. The word "comprising" or "comprises", and the like, is intended to mean that elements or items that are present in front of "comprising" or "comprising" are included in the word "comprising" or "comprising", and equivalents thereof, without excluding other elements or items. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.
In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, etc. are defined with respect to the configuration shown in the drawings, and in particular, "height" corresponds to the top-to-bottom dimension, "width" corresponds to the left-to-right dimension, and "depth" corresponds to the front-to-back dimension, are relative concepts, and thus may vary accordingly depending on the location and use of the terms, and therefore these or other orientations should not be interpreted as limiting terms.
Terms (e.g., "connected" and "attached") relating to attachment, coupling, and the like refer to a relationship wherein these structures are directly or indirectly secured or attached to one another through intervening structures, as well as to a relationship wherein they are movably or rigidly attached, unless expressly stated otherwise.
As can be seen in conjunction with the illustrations of fig. 1 and 2, a homogeneous immunoassay analyzer according to an embodiment of the present invention includes: the sample storage and conveying module 1, the reaction container storage and loading module 2, the reagent storage and conveying module 3, the incubation module 4 and the detection module 5 are arranged above the operation table;
the sample storage and conveying module 1 is positioned at one end of the operation table, the reagent storage and conveying module 3 is positioned at the other end of the operation table, the incubation module 4 is positioned in the middle of the operation table, and the reaction container storage and loading module 2 and the detection module 5 are both arranged beside the incubation module 4;
The reaction container storage and loading module 2 loads the reaction container to the incubation module 4, the sample storage and conveying module 1 conveys the sample to be detected to the reaction container on the incubation module 4, the reagent storage and conveying module 3 conveys the detection reagent to the reaction container on the incubation module 4, so that the detection reagent is mixed with the sample to be detected, and the detection module 5 measures and detects the mixed liquid in the reaction container.
In a preferred embodiment, the homogeneous immunoassay analyzer further comprises a waste storage module comprising a solid waste storage unit for storing the reaction vessel after the analytical test and a waste storage unit for storing the mixed liquor after the analytical test.
The solid waste storage unit and the waste liquid storage unit are used for respectively analyzing and detecting the reaction container and the mixed liquid, so that the reaction container and the mixed liquid after analysis and detection are prevented from being discharged in a mess, and environmental pollution is caused.
Further, the sample storage and delivery module 1 includes: the sample conveying line 12 is sequentially provided with an emergency treatment feeding station, a sample identification station, a sample loading station and a sample recovery station along the conveying direction of the sample conveying line 12;
the sample conveying unit 11 is arranged beside the sample conveying line 12, and the sample conveying unit 11 is positioned at a sample feeding station;
The sample identification module 14 is arranged beside the sample conveying line 12, and the sample identification module 14 is positioned at a sample identification station; and
The sample recovery unit 13 is arranged beside the sample conveying line 12, and the sample recovery unit 13 is positioned at a sample recovery station;
The sample conveying unit 11 is used for conveying a sample to be detected to a sample feeding station, the sample identifying module 14 is used for identifying the sample to be detected, and the sample recycling unit 13 is used for recycling the sample.
In a preferred embodiment, the sample delivery unit 11 comprises: the sample loading area is in butt joint with the sample conveying line 12, a sample rack is placed in the first sample buffer area, and a sample to be detected is placed in the sample rack; the sample holder is placed in the first sample buffer where it is transported to a sample loading zone and then to a sample loading station of the sample transport line 12.
The sample collection unit 13 includes: the sample unloading area is in butt joint with the sample conveying line 12, the sample rack after being taken is conveyed to a recovery station through the sample conveying line 12, and the sample rack is conveyed to the second sample buffer area through the sample unloading area for recovery and storage.
In a preferred embodiment of the present invention, the sample identification module 14 is a code scanner, and the surface of the sample holder is provided with an identification code, and the code scanner scans the identification code on the surface of the sample holder to identify the information of the sample to be detected.
Further, the sample storage and delivery module 1 further includes: the emergency loading unit 15 is positioned at the emergency loading station; and
A sample application unit 16 located at the sample application station, the sample application unit 16 delivering a sample located at the sample application station into a reaction vessel on the incubation module 4.
In one embodiment of the present invention, the sample loading unit 16 is a mechanical arm structure, and in another embodiment of the present invention, the sample loading unit 16 is a linear guide mechanism.
The sample loading unit 16 specifically selects a mechanical arm structure or a linear guide rail mechanism, and a user can select the mechanical arm structure or the linear guide rail mechanism according to actual needs.
The sample conveying line 12 is provided with the emergency treatment loading station, the emergency treatment loading unit 15 is arranged at the emergency treatment loading station, a user can place samples to be detected which need to be subjected to emergency treatment on the emergency treatment loading unit 15, the samples are conveyed to the emergency treatment loading station of the sample conveying line 12 by the emergency treatment loading unit 15, and the samples to be detected which need to be subjected to emergency treatment are prevented from being placed in a first sample buffer area to be subjected to queuing and conveying detection when conveyed by the sample conveying line 12, so that the working efficiency is improved, and the working time is saved.
It will be appreciated that the sample holder containing the sample to be detected is placed in the first sample buffer zone, transported to the sample loading station of the sample transport line 12 via the sample loading zone, transported to the sample recognition station via the sample transport line 12, the sample to be detected is recognized by the sample recognition module 14 at the sample recognition station, after recognition, the sample to be detected is transported to the sample loading station, the sample at the sample loading station is transported to the reaction vessel on the incubation module 4 by the sample loading unit 16, the sample holder is transported to the recovery station, and transported to the second sample buffer zone for recovery and storage via the sample unloading zone at the recovery station.
Further, the reaction vessel storage loading module 2 includes: a reaction vessel storage tray 21, on which at least two reaction vessels are stored; and
A movable gripping module 22 provided beside the reaction container storage tray 21;
the movable grabbing module 22 grabs and places the reaction containers stored on the reaction container storage tray 21 onto the incubation module 4 sequentially.
In a preferred embodiment, the movable gripping module 22 is provided with a material presence sensor for detecting whether the reaction container is stored in the reaction container storage tray 21,
The reaction vessel storage tray 21 is provided with an empty alarm which gives an empty alarm when the reaction vessel storage tray 21 is judged to be in an empty state.
The empty alarm may be light, sound, image, text, etc., and in a preferred embodiment of the present invention, the empty alarm is sound.
It is to be understood that the mobile grabbing module 22 detects the reaction container storage tray 21 through the material presence sensor, when the material presence sensor senses that the reaction container storage tray 21 does not have a reaction container, the reaction container storage tray 21 is judged to be in an empty state, the empty alarm sends out an empty alarm, and when the material presence sensor senses that the reaction container storage tray 21 has a reaction container, the mobile grabbing module 22 clamps and places the reaction container on the incubation module 4.
Further, the reagent storage and delivery module 3 includes: the reagent rotary table 31 is provided with at least two reagent containers above, wherein detection reagents are stored in the reagent containers, and the reagent rotary table 31 is sequentially provided with a reagent recognition station and a reagent sample adding station along the rotation direction;
The reagent identification module 33, the reagent identification module 33 is arranged beside the reagent turntable 31, and the reagent identification module 33 is positioned at the reagent identification station; and
The reagent adding module 32, the reagent adding module 32 is arranged beside the reagent turntable 31, and the reagent adding module 32 is positioned at the reagent adding station;
The reagent identifying module 33 identifies the reagent container located at the reagent identifying station, and thus identifies the detection reagent in the reagent container, and the reagent loading module 32 loads the detection reagent in the reagent container located at the reagent loading station into the reaction container.
In a preferred embodiment, the reagent identifying module 33 is a code scanner, and the surface of the reagent container is provided with an identifying code, and the code scanner scans the identifying code on the surface of the reagent container to identify the information of the detection reagent in the reagent container.
The reagent identification module 33 identifies the relevant information of the detection reagent, so that the reagent sample adding module 32 can select different detection reagents for detection according to different samples to be detected, and the reagent identification module 33 is prevented from loading wrong detection reagents to be mixed with the samples to be detected, thereby causing waste and increasing detection time.
The bottom of the reagent rotary disc 31 is provided with a rotary disc driver, and the rotary disc driver drives the reagent rotary disc 31 to rotate.
In one embodiment of the present invention, the reagent sample module 32 is a mechanical arm structure, and in another embodiment of the present invention, the reagent sample module 32 is a linear guide mechanism.
The reagent sample adding module 32 specifically selects a mechanical arm structure or a linear guide rail mechanism, and a user can select the reagent sample adding module according to actual needs.
It will be appreciated that different detection reagents are stored in different reagent containers, the reagent turntable 31 drives the reagent container containing the detection reagent to the reagent recognition station, the reagent recognition module 33 recognizes the relevant information of the detection reagent, the reagent container containing the detection reagent is driven to the reagent loading station by the reagent turntable 31, and the reagent loading module 32 loads the detection reagent in the reagent container at the reagent loading station into the reaction container on the incubation module 4, and mixes with the sample to be detected in the reaction container.
Further, the incubation module 4 is an incubation turntable, at least two placement parts are arranged above the incubation turntable, each placement part is regularly arrayed along the periphery of the incubation turntable, and the placement parts are used for placing reaction containers;
The rotary table is provided with a reaction container feeding station, a sample feeding station, a reagent feeding station, an incubation station and a detection station along the rotation direction of the rotary table;
the reaction container storage loading module 2 is located at the reaction container loading station, the sample loading unit 16 is located at the sample loading station, the reagent loading module 32 is located at the reagent loading station, and the detection module 5 is located at the detection station.
Further, the temperature control module is arranged below the incubation module 4, and the temperature control module is used for accurately controlling the incubation temperature of the incubation module 4, so that the mixed solution of the sample to be detected and the detection reagent has a good incubation effect, and the subsequent detection module is convenient to detect.
In a preferred embodiment of the present invention, the temperature control module employs peltier.
It can be understood that the reaction container is placed on the placing portion of the incubation module 4 at the reaction container loading station by the moving grabbing module 22, the incubation module 4 is conveyed to the sample loading station, the sample to be detected is conveyed into the reaction container located at the sample loading station by the sample loading unit 16, the reaction container containing the sample to be detected is conveyed to the reagent loading station, the corresponding detection reagent is loaded into the reaction container containing the sample to be detected located at the reagent loading station by the reagent loading module 32, mixed with the sample to be detected, the reaction container containing the mixed liquid to be detected is conveyed to the incubation station, after incubation at the incubation station is completed, the reaction container containing the mixed liquid to be detected is conveyed to the detection station, and detection is performed by the detection module 5.
Further, the detection module 5 includes: photon counting unit 51, filter assembly 52, dichroic mirror 53, light source 54, and light source filter 55;
The dichroic mirror 53 is obliquely arranged, the dichroic mirror 53 is positioned right above the detection station, the light source is arranged at the side of the dichroic mirror 53, the light source filter 55 is arranged between the light source 54 and the dichroic mirror 53, the photon counting unit 51 and the filter component 52 are both arranged above the dichroic mirror 53, and the filter component 52 is arranged between the photon counting unit 51 and the dichroic mirror 53;
the light source 54 provides a detection light source, the filter assembly 52 and the light source filter 55 are used to select light of a specific wavelength, and the photon counting unit 51 collects the final photon signal.
In a preferred embodiment of the present invention, the filter assembly 52 includes: the optical filter switcher is driven by the rotating motor to complete the switching of the first optical filter and the second optical filter so as to detect the luminous intensity of the sample to be detected with two different wavelengths.
In a preferred embodiment of the present invention, the center wavelength of the light source filter 55 is 320nm, the center wavelength of the first filter is 620nm, and the center wavelength of the second filter is 655nm.
It will be appreciated that at the detection station, the light source 54 emits a detection light source, the detection light source passes through the light source filter 55, the light source filter 55 selects a specific wavelength of light to the dichroic mirror 53, a part of the light is refracted by the dichroic mirror 53 into the reaction vessel of the detection station, another part of the light is refracted by the dichroic mirror 53 into the first filter, and the photon counting unit 51 collects a corresponding photon signal after passing through the first filter to the photon counting unit 51; after the collection is completed, the optical filter switcher switches the first optical filter to the second optical filter, the above operation is repeated, the photon counting unit 51 collects the photon signals corresponding to the second optical filter, and after the photon counting unit 51 collects two different photon signals, the photon signals are processed through the corresponding algorithm, so as to obtain the corresponding detection information of the sample to be detected.
In summary, the working principle of the homogeneous immunoassay analyzer of the present invention is as follows:
The mobile grabbing module 22 detects the reaction container storage tray 21 through a material presence sensor, when the material presence sensor senses that the reaction container storage tray 21 does not have a reaction container, the empty alarm gives out an empty alarm when the reaction container storage tray 21 is judged to be in an empty state, and when the material presence sensor senses that the reaction container storage tray 21 has a reaction container, the mobile grabbing module 22 clamps the reaction container to a reaction container feeding station on the incubation module 4 and conveys the reaction container to the sample feeding station through the incubation module 4;
The sample rack filled with the sample to be detected is placed in the first sample buffer zone, the sample is conveyed to a sample loading station of the sample conveying line 12 through the sample loading zone, the sample is conveyed to a sample recognition station through the sample conveying line 12, the sample to be detected is recognized by the sample recognition module 14 at the sample recognition station, the sample to be detected is continuously conveyed to the sample loading station after the recognition is finished, the sample at the sample loading station is loaded into a reaction container at the sample loading station of the incubation module 4 through the sample loading unit 16, the reaction container filled with the sample to be detected is conveyed to a reagent loading station through the incubation module 4, the sample rack is continuously conveyed to a recovery station, and the sample is conveyed to a second sample buffer zone through the sample unloading zone at the recovery station for recovery and storage;
the reagent turntable 31 drives a reagent container filled with detection reagent to a reagent recognition station, the reagent recognition module 33 recognizes relevant information of the detection reagent, the reagent container filled with the detection reagent is driven to a reagent sample adding station by the reagent turntable 31, and the reagent sample adding module 32 loads the detection reagent in the reagent container at the reagent sample adding station into a reaction container at a reagent sample adding station of the incubation module 4 and mixes the detection reagent with a sample to be detected in the reaction container;
the reaction container filled with the mixed liquid to be tested is conveyed to an incubation station, incubation is carried out at the incubation station, and after incubation is completed, the reaction container filled with the mixed liquid to be tested is conveyed to a detection station, and detection is carried out by a detection module 5;
At the detection station, the light source 54 emits a detection light source, the detection light source passes through the light source filter 55, the light source filter 55 selects light with a specific wavelength to the dichroic mirror 53, one part of the light is refracted to the reaction container of the detection station through the dichroic mirror 53, the other part of the light is refracted to the first filter through the dichroic mirror 53, and the photon counting unit 51 collects corresponding photon signals after passing through the first filter to the photon counting unit 51; after the collection is completed, the optical filter switcher switches the first optical filter to the second optical filter, the above operation is repeated, the photon counting unit 51 collects the photon signals corresponding to the second optical filter, and after the photon counting unit 51 collects two different photon signals, the photon signals are processed through the corresponding algorithm, so as to obtain the corresponding detection information of the sample to be detected.
The number of equipment and the scale of processing described herein are intended to simplify the description of the present invention. Applications, modifications and variations of the present invention will be readily apparent to those skilled in the art.
Although embodiments of the invention have been disclosed above, they are not limited to the use listed in the specification and embodiments. It can be applied to various fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. Therefore, the invention is not to be limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.
Claims (7)
1. A homogeneous immunoassay analyzer, comprising: an operation table; and
The sample storage and conveying module (1), the reaction container storage and loading module (2), the reagent storage and conveying module (3), the incubation module (4) and the detection module (5) are arranged above the operation table;
the sample storage and conveying module (1) is positioned at one end of the operating platform, the reagent storage and conveying module (3) is positioned at the other end of the operating platform, the incubation module (4) is positioned in the middle of the operating platform, and the reaction container storage and loading module (2) and the detection module (5) are arranged beside the incubation module (4);
The reaction container storage and loading module (2) loads a reaction container to the incubation module (4), the sample storage and conveying module (1) conveys a sample to be detected into the reaction container positioned on the incubation module (4), the reagent storage and conveying module (3) conveys a detection reagent into the reaction container positioned on the incubation module (4) so that the detection reagent is mixed with the sample to be detected, and the detection module (5) measures and detects the mixed liquid in the reaction container;
The sample storage and delivery module (1) comprises: the sample conveying line (12), the sample conveying line (12) is sequentially provided with an emergency treatment feeding station, a sample identification station, a sample loading station and a sample recovery station along the conveying direction;
The sample transport unit (11) comprises: the sample loading area is in butt joint with the sample conveying line (12), a sample frame is placed in the first sample buffer area, and a sample to be detected is placed in the sample frame; the sample rack is placed in the first sample buffer zone, is conveyed to a sample loading zone in the first sample buffer zone and is conveyed to a sample loading station of the sample conveying line (12);
The emergency loading unit (15) is positioned at the emergency loading station, a sample to be detected which needs emergency treatment is placed in the emergency loading unit (15), the sample is conveyed to the emergency loading station of the sample conveying line (12) by the emergency loading unit (15), and then conveyed to the sample identification station by the sample conveying line (12), and the sample to be detected is continuously conveyed to the sample loading station after identification is completed;
A sample application unit (16) located at the sample application station, the sample application unit (16) delivering a sample located at the sample application station into a reaction vessel on the incubation module (4).
2. Homogeneous immunoassay according to claim 1, wherein said sample storage and delivery module (1) further comprises:
the sample conveying unit (11) is arranged beside the sample conveying line (12), and the sample conveying unit (11) is positioned at a sample feeding station;
the sample identification module (14) is arranged beside the sample conveying line (12), and the sample identification module (14) is positioned at a sample identification station; and
The sample recovery unit (13) is arranged beside the sample conveying line (12), and the sample recovery unit (13) is positioned at a sample recovery station;
The sample conveying unit (11) is used for conveying a sample to be detected to the sample feeding station, the sample identifying module (14) is used for identifying the sample to be detected, and the sample recycling unit (13) is used for recycling the sample.
3. Homogeneous immunoassay according to claim 1, wherein said reaction vessel storage loading module (2) comprises: a reaction vessel storage tray (21), at least two reaction vessels being stored on the reaction vessel storage tray (21); and
A movable gripping module (22) provided beside the reaction vessel storage tray (21);
the movable grabbing module (22) grabs and places the reaction containers stored on the reaction container storage disc (21) on the incubation module (4) in sequence.
4. Homogeneous immunoassay according to claim 1, wherein said reagent storage and delivery module (3) comprises: the reagent rotary table (31) is provided with at least two reagent containers above, detection reagents are stored in the reagent containers, and the reagent rotary table (31) is sequentially provided with a reagent recognition station and a reagent sample adding station along the rotation direction;
the reagent identification module (33), the reagent identification module (33) is arranged beside the reagent turntable (31), and the reagent identification module (33) is positioned at the reagent identification station; and
The reagent sample adding module (32), the reagent sample adding module (32) is arranged beside the reagent rotary table (31), and the reagent sample adding module (32) is positioned at the reagent sample adding station;
the reagent identification module (33) identifies the reagent container located at the reagent identification station so as to identify the detection reagent in the reagent container, and the reagent sample adding module (32) loads the detection reagent in the reagent container located at the reagent sample adding station into the reaction container.
5. Homogeneous immunoassay according to claim 1, wherein the incubation module (4) is an incubation carousel, over which at least two placement portions are provided, each of which is regularly arrayed along the periphery of the incubation carousel, the placement portions being for placement of reaction vessels;
The rotary table is provided with a reaction container feeding station, a sample feeding station, a reagent feeding station, an incubation station and a detection station along the rotation direction of the rotary table;
The reaction container storage loading module (2) is located at the reaction container loading station, the sample loading unit (16) is located at the sample loading station, the reagent loading module (32) is located at the reagent loading station, and the detection module (5) is located at the detection station.
6. Homogeneous immunoassay analyzer according to claim 1, characterized in that a temperature control module is arranged below the incubation module (4), and the temperature control module is used for precisely controlling the incubation temperature of the incubation module (4).
7. Homogeneous immunoassay according to claim 5, wherein said detection module (5) comprises: a photon counting unit (51), a filter assembly (52), a dichroic mirror (53), a light source (54) and a light source filter (55);
The light source is arranged beside the dichroic mirror (53), the light source filter (55) is arranged between the light source (54) and the dichroic mirror (53), the photon counting unit (51) and the filter component (52) are arranged above the dichroic mirror (53), and the filter component (52) is arranged between the photon counting unit (51) and the dichroic mirror (53);
The light source (54) provides a detection light source, the filter assembly (52) and the light source filter (55) are used for selecting light with specific wavelength, and the photon counting unit (51) collects final photon signals.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104350386A (en) * | 2012-06-11 | 2015-02-11 | 株式会社日立高新技术 | Automatic analysis apparatus |
| WO2019235158A1 (en) * | 2018-06-06 | 2019-12-12 | 株式会社日立ハイテクノロジーズ | Automated analysis device, and method for conveying sample |
| CN214473391U (en) * | 2020-11-26 | 2021-10-22 | 南京国科医工科技发展有限公司 | A homogeneous immunoassay analyzer |
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| JP2012173251A (en) * | 2011-02-24 | 2012-09-10 | Sysmex Corp | Sample analysis device and sample analysis method |
| KR20150119365A (en) * | 2013-02-18 | 2015-10-23 | 테라노스, 인코포레이티드 | Systems and methods for multi-analysis |
| CN111855985A (en) * | 2020-08-03 | 2020-10-30 | 宋世琦 | Analysis meter |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350386A (en) * | 2012-06-11 | 2015-02-11 | 株式会社日立高新技术 | Automatic analysis apparatus |
| WO2019235158A1 (en) * | 2018-06-06 | 2019-12-12 | 株式会社日立ハイテクノロジーズ | Automated analysis device, and method for conveying sample |
| CN214473391U (en) * | 2020-11-26 | 2021-10-22 | 南京国科医工科技发展有限公司 | A homogeneous immunoassay analyzer |
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