CN112400630A - A method for reducing the content of H2O2 and MDA in non-heading Chinese cabbage after low temperature stress - Google Patents
A method for reducing the content of H2O2 and MDA in non-heading Chinese cabbage after low temperature stress Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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Abstract
本发明公开了一种降低低温胁迫后不结球白菜内H2O2和MDA含量的方法，涉及遗传育种技术领域，喷施0.1μmol·L‑1油菜素内酯(BRs)和0.1μmol·L‑1独角金内酯(SL)的混合液(含0.1％吐温80)。对照为清水喷施，和BRs、SL喷施。BRs、SL浓度同上。所有处理在低温前两天的8：00和20：00分别喷施，喷施部位为叶片背面，喷施至叶片表面滴水为止。共喷施4次；本发明提供的混合液可有效提高不结球白菜幼苗的抗低温胁迫性能，为提高不结球白菜低温胁迫后的品质和产量提供了有效的指导思路。
The invention discloses a method for reducing the content of H 2 O 2 and MDA in non - heading Chinese cabbage after low temperature stress, and relates to the technical field of genetics and breeding. L -1 Strigolactone (SL) mixture (containing 0.1% Tween 80). The control was sprayed with water, and sprayed with BRs and SL. The concentrations of BRs and SL are the same as above. All treatments were sprayed respectively at 8:00 and 20:00 two days before the low temperature, and the spraying site was the back of the leaves until the surface of the leaves dripped water. A total of 4 times of spraying; the mixed solution provided by the invention can effectively improve the low temperature stress resistance performance of non-heading cabbage seedlings, and provides an effective guiding idea for improving the quality and yield of non-heading cabbage after low temperature stress.
Description
Technical Field
The invention relates to the technical field of vegetable seedling culture, in particular to a method for relieving low-temperature stress injury of non-heading Chinese cabbage seedlings.
Background
Non-heading Chinese cabbage is a variety of Brassica species of Brassicaceae, Brassica, and Brassica subspecies, is an important vegetable in autumn and winter, and is cultivated in most areas of China, especially in Yanghuai river basin. The non-heading Chinese cabbage has beautiful appearance, is rich in various vitamins and has high nutritional value, so the non-heading Chinese cabbage is deeply popular with people and increasingly becomes popular vegetables.
At present, low-temperature stress is a main environmental factor which seriously affects the growth and development of plants and the crop yield, after the non-heading Chinese cabbage plants are stressed by low temperature, the growth of the non-heading Chinese cabbage plants is obviously inhibited, the fresh weight and the dry weight of overground parts of the plants are quickly reduced, the normal growth and development of the non-heading Chinese cabbage are seriously affected, the yield is greatly reduced, the quality is reduced, and therefore great economic loss is brought to farmers and the market supply is affected.
Hydrogen peroxide (H)2O2) Is active oxygen, can cause oxidative damage to plant cells, and the increase of the content of the active oxygen can increase membrane lipid peroxidation; malondialdehyde (MDA) is a lipid peroxidation product, and the level of its content is used to assess the degree of lipid peroxidation; the low content indicates that the degree of oxidative damage of plant cell membranes and organelle membranes is low, and the tolerance to low temperature is higher.
Therefore, the method for improving the cold resistance of the non-heading Chinese cabbage by reducing the content of hydrogen peroxide and malondialdehyde in the non-heading Chinese cabbage can provide a research and development direction for reducing the low-temperature stress on the yield of the non-heading Chinese cabbage and the like.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a method for reducing H in non-heading Chinese cabbage after low-temperature stress2O2And the MDA content, so as to solve the technical problem that the non-heading Chinese cabbage has weak low-temperature stress resistance in the prior art.
The invention is realized by the following technical scheme:
the invention provides a method for reducing H in non-heading Chinese cabbage after low-temperature stress2O2And MDA content, which comprises spraying a mixed solution of brassinolide and strigolactone during seedling stage of non-heading Chinese cabbage.
Furthermore, Tween 80 is also included in the mixed solution.
Further, the period of spraying the mixed liquid is that the non-heading Chinese cabbage grows to 5-6 leaves.
Further, it is characterized in that the concentration of the brassinolide in the mixed solution is 0.1. mu. mol. L-1The concentration of strigolactone is 0.1. mu. mol. L-1The dosage of the Tween 80 is 0.1 percent of the mixed solution.
Further, the spraying part of the mixed solution is the back of the non-heading Chinese cabbage leaves until the surface of the leaves drips.
Compared with the prior art, the invention has the following advantages:
1. the method is simple to operate, convenient to implement and free from region limitation;
2. during seedling period, spraying the mixed solution of brassinolide and strigolactone, which can effectively reduce the content of hydrogen peroxide and malondialdehyde in the non-heading Chinese cabbage seedlings after low-temperature stress, thereby realizing the protection of the seedlings, further increasing the low-temperature stress resistance of the non-heading Chinese cabbage and being beneficial to increasing the yield of the non-heading Chinese cabbage;
3. the brassinolide and the strigolactone belong to environment-friendly reagents, have no pollution to the environment and are convenient to use.
Drawings
FIG. 1 is a graph showing the results of detection in example 1;
FIG. 2 is a graph showing the results of detection in example 2;
FIG. 3 is a diagram showing the results of the detection in example 3.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Concentration, time and mode of plant planting and spraying mixed liquid
After germination in the plug, the seeds were transplanted into a plug containing matrix and vermiculite (3:1, v/v). Seedlings were grown at 25/18 deg.C (day/night) at 300. mu. mol. m-2s-1The growth was carried out in a growth chamber at light intensity and 70% relative humidity with a photoperiod of 12:12 h. When the seedlings grow to 5-6 leaves (45 days after planting), selecting uniform seedlings, and spraying 0.1 mu mol. L-1Brassinolide (BRs) and 0.1. mu. mol. L-1A mixture of Strigolactone (SL) (containing 0.1% tween 80). The control is spraying with clear water, and spraying with BRs and SL. BRs and SL concentrations are as above. Two days before cold for all treatments 8: 00 and 20: 00 spraying respectively, wherein the spraying part is the back of the leaves and is sprayed until the surface of the leaves drips. Sprayed for 4 times in total.
After 5 days of low temperature treatment, samples from the five treatment groups were collected, snap frozen with liquid nitrogen and stored in a-80 ℃ freezer for subsequent testing. Firstly, the content of hydrogen peroxide and malondialdehyde is detected for each group of non-heading Chinese cabbage seedlings, and the specific detection method is as in example 1.
Example 1
The mixed solution is used for the seedling H of the non-heading Chinese cabbage at low temperature2O2And Effect of MDA content
According to Solibao' hydrogen peroxide H2O2) Content detection kit' instruction book for determining H in sample2O2And (4) content. And (3) measuring the content of MDA in the sample by using a thiobarbituric acid method. As can be seen from FIG. 1, after a low temperature, the seedling leaves H of non-heading Chinese cabbage2O2The content and the MDA content are obviously increased, the content of EBR or SL sprayed independently can be reduced to different degrees, the content of EBR and SL sprayed in a mixed mode in plant leaves is obviously reduced, and the difference between the content and the normal temperature is not large.
Simultaneously, hydrogen peroxide (H)2O2) Is active oxygen, can cause oxidative damage to plant cells, and the increase of the content of the active oxygen can increase membrane lipid peroxidation; malondialdehyde (MDA) is a lipid peroxidation product, and the level of its content is used to assess the degree of lipid peroxidation; the low content indicates that the degree of oxidative damage of plant cell membranes and organelle membranes is low, and the tolerance to low temperature is higher.
The result shows that the spraying of the EBR and SL mixed solution before low-temperature stress can obviously reduce the H under the low-temperature stress2O2And the content of MDA, thereby reducing the oxidative damage of low temperature to cells, namely improving the low temperature stress resistance of the non-heading Chinese cabbage.
In order to further analyze the improvement of the low temperature stress resistance of the seedlings of the non-heading Chinese cabbage by spraying the mixed solution, the following examples are also carried out.
Example 2
Influence of mixed solution on T-AOC and RWC content of non-heading Chinese cabbage seedlings at low temperature
The measurement of the total antioxidant capacity refers to the total antioxidant level formed by various antioxidant substances, antioxidant enzymes and the like in a sample, and the measurement of plant leaves collected after the low-temperature treatment for 5 days is carried out by using a Solebao total antioxidant capacity (T-AOC) detection kit. The relative water content of the plant leaves is an important index of the water condition of plant tissues, and the selection of counter-stress breeding has special significance; the dry and fresh weight of the leaves is used for measuring the weight. The detection result is shown in figure 2, the total antioxidant capacity (T-AOC) and the Relative Water Content (RWC) of the leaves of the seedlings of the non-heading Chinese cabbage are obviously reduced, the content of EBR or SL sprayed alone is improved to different degrees, the content of EBR and SL sprayed in a mixed mode is improved to the maximum extent in the leaves of the plants, the T-AOC is obviously improved compared with that under the normal temperature condition, and the difference between RWC and the normal temperature is small. The result shows that the total antioxidant capacity and the relative water content of the seedlings of the non-heading Chinese cabbage can be obviously improved by spraying the EBR and SL mixed solution under the low-temperature stress.
Example 3
Influence of mixed liquid on photosynthesis mechanism of non-heading Chinese cabbage seedling at low temperature
After 5 days of low temperature treatment, fluorescence emitted from chlorophyll in plant leaves after dark adaptation for 30min was measured using a continuous excitation fluorometer plant efficiency instrument (PEA, Hansatech, UK), and the results are shown in fig. 3.
VjCan reflect the closing degree of the active reaction center when the reaction center is irradiated for 2 ms. PI (proportional integral)absThe photosynthetic performance index can reflect the state of the plant photosynthetic mechanism, and the parameters are sensitive, so that the influence of stress on the photosynthetic mechanism can be well reflected. As can be seen from the above figure, PI after low temperatureabsThe rapid reduction shows that the low temperature has influence on the structure of the photo-curing machine, and the EBR or SL is sprayed alone and then is improved to a small extent, but is much different from the room temperature state; after EBR and SL are sprayed in a mixed way, the temperature is restored to a level which is not much different from the normal temperature. VjThe sharp rise indicates that the active reaction center is quickly closed after low temperature, and the EBR or SL is sprayed alone and then is reduced to a small extent, but the difference is more than that in the normal temperature state; after EBR and SL are sprayed in a mixed way, the temperature is restored to a level which is not much different from the normal temperature. The result shows that the influence of low temperature on the photosynthetic mechanism can be obviously reduced by spraying the EBR and SL mixed solution under the low-temperature stress.
The results obtained in examples 1 to 3 are shown in Table 1
TABLE 1
Through the graphs 1-3 (in the graphs, Cont is normal temperature, LT is low temperature, both normal temperature and low temperature are spraying clear water and adding tween 80, tween 80 is a surfactant and can enable plant leaves to better absorb exogenous sprayed hormone.) and table 1, the invention can effectively reduce the content of hydrogen peroxide and malondialdehyde in the non-heading cabbage seedlings after low-temperature stress by spraying the mixed solution before the low-temperature stress comes, thereby reducing the damage of the low temperature to the seedlings, simultaneously reducing the influence of the low temperature to other performances of the seedlings by spraying the mixed solution, further integrally increasing the low-temperature stress resistance of the seedlings, and providing a thought for reducing the influence of the low-temperature stress to the non-heading cabbage seedlings and improving the quality and yield of the seedlings.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. Non-heading Chinese cabbage H capable of reducing low-temperature stress2O2And MDA content, characterized in that the method comprises spraying a mixed solution of brassinolide and monolinolide during the seedling stage of non-heading Chinese cabbage.
2. The method of claim 1 for reducing H in non-heading Chinese cabbage after low temperature stress2O2And MDA content, characterized in that the mixed solution also comprises Tween 80.
3. The method of claim 2 for reducing H in non-heading Chinese cabbage after low temperature stress2O2And MDA content, characterized in that the period of spraying the mixed solution is the period of the non-heading Chinese cabbage growing to 5-6 leaves.
4. The method of claim 3 for reducing H in non-heading Chinese cabbage after low temperature stress2O2And MDA content, characterized in that the concentration of brassinolide in the mixed solution is 0.1. mu. mol. L-1The concentration of strigolactone is 0.1. mu. mol. L-1Tween 80 was used in an amount of 0.1% of the total volume of the final mixture.
5. The method according to claim 4, wherein the mixed solution is sprayed on the back of the leaves of the non-heading Chinese cabbage until water drops on the surface of the leaves.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112430600A (en) * | 2020-11-23 | 2021-03-02 | 安徽农业大学 | High and low temperature stress resistance related gene of non-heading Chinese cabbage and application thereof |
| CN112430601A (en) * | 2020-11-23 | 2021-03-02 | 安徽农业大学 | Cold resistance related gene of non-heading Chinese cabbage and application thereof |
| CN114467730A (en) * | 2022-02-25 | 2022-05-13 | 安徽农业大学 | Method for increasing contents of nicotinamide and trehalose of non-heading Chinese cabbage |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112430600A (en) * | 2020-11-23 | 2021-03-02 | 安徽农业大学 | High and low temperature stress resistance related gene of non-heading Chinese cabbage and application thereof |
| CN112430601A (en) * | 2020-11-23 | 2021-03-02 | 安徽农业大学 | Cold resistance related gene of non-heading Chinese cabbage and application thereof |
| CN112430601B (en) * | 2020-11-23 | 2022-03-29 | 安徽农业大学 | A gene related to cold tolerance of non-heading Chinese cabbage and its application |
| CN112430600B (en) * | 2020-11-23 | 2022-05-31 | 安徽农业大学 | High and low temperature stress resistance related gene of non-heading Chinese cabbage and application thereof |
| CN114467730A (en) * | 2022-02-25 | 2022-05-13 | 安徽农业大学 | Method for increasing contents of nicotinamide and trehalose of non-heading Chinese cabbage |
| CN114467730B (en) * | 2022-02-25 | 2022-11-15 | 安徽农业大学 | A method for increasing the content of nicotinamide and trehalose in non-heading cabbage |
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Application publication date: 20210226 |