CN112391439B - Method for detecting disposable sterilization rate of air disinfection equipment - Google Patents
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- CN112391439B CN112391439B CN202011177652.4A CN202011177652A CN112391439B CN 112391439 B CN112391439 B CN 112391439B CN 202011177652 A CN202011177652 A CN 202011177652A CN 112391439 B CN112391439 B CN 112391439B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/015—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
- A61L2209/211—Use of hydrogen peroxide, liquid and vaporous
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
- A61L2209/212—Use of ozone, e.g. generated by UV radiation or electrical discharge
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention discloses a method for detecting the one-time sterilization rate of air disinfection equipment, which comprises the following steps of firstly, respectively connecting an air inlet and an air outlet of equipment to be detected to an emergency sampling air bag of a bacteria-infected air bag; then, filling a preset amount of air and a preset amount of aerosol into the bacteria-infected air bag, and standing for a specified time; next, opening the equipment to be detected, and enabling all air in the bacteria-contaminated air bag to enter the sampling air bag after being disinfected by the equipment to be detected; then, sampling the air in the sampling air bag, and calculating the aerosol content of the sampled air so as to obtain the one-time sterilization rate of the equipment to be detected; and finally, sterilizing the equipment to be detected, the bacteria-contaminated air bag and the sampling air bag.
Description
Technical Field
The invention relates to an air sterilization technology, in particular to a method for detecting the one-time sterilization rate of air sterilization equipment.
Background
According to the requirements of the relevant policies of the State health administration, in order to guarantee the life health and safety of people, the air disinfection equipment needs to obtain third-party detection and authentication before being put into the market. One of the detection standards of the air disinfection equipment is the sterilization rate, namely the efficiency of killing microorganisms (pathogenic bacteria, viruses and the like) in the air, and is used for reflecting the sterilization performance of the air disinfection equipment. The sterilization rate detection requires a necessary detection method and detection apparatus. Currently, the sterilization rate detection method is generally to perform detection in a circulating sterilization mode in a closed aerosol chamber. The sterilization rate measured by this method is called the cyclic disinfection sterilization rate. In the implementation process of the detection method, the disinfected air and the unsterilized air are mixed in the aerosol chamber, so that the disposable sterilization rate of the air disinfection equipment cannot be detected, and the most direct judgment on the sterilization capacity of the air disinfection equipment is difficult to make.
Disclosure of Invention
To solve some or all of the problems in the prior art, the present invention provides a method for detecting a disposable sterilization rate of an air sterilizer, comprising:
a connection device, comprising: connecting an air inlet of the equipment to be detected to the bacteria-contaminated air bag, and connecting an air outlet of the equipment to be detected to the sampling air bag;
the bacterial contamination operation comprises the following steps: filling a preset amount of air into the bacteria-infected air bag, then spraying a preset amount of aerosol, and standing for a specified time;
a sterilization operation comprising: opening the equipment to be detected, and enabling air in the bacteria-infected air bag to enter the sampling air bag after being disinfected by the equipment to be detected; and
sampling and calculating the sterilization rate, including: and after all the gas in the bacteria-contaminated air bag enters the sampling air bag, sampling the air in the sampling air bag, and calculating the aerosol content of the sampled air so as to obtain the one-time sterilization rate of the equipment to be detected.
Further, the method further comprises terminal sterilization comprising: and sterilizing the device to be detected and/or the bacteria-infected air bag and/or the sampling air bag.
Further, the air bag for dying bacteria comprises a bacteria spraying opening, a sterilizing material spraying opening, a pressure gauge, an air pump and an air outlet air valve.
Further, the air inlet of the equipment to be detected is connected to the air outlet air valve of the bacteria-contaminated air bag, the air outlet air valve is in a closed state during bacteria-contaminated operation, the air outlet air valve is opened during disinfection operation, and the air outlet air valve is closed again after the disinfection operation is completed.
Further, the bacterial contamination air bag also comprises an observation window, an airtight zipper and an inflatable bottom frame.
Furthermore, the sampling air bag comprises a disinfectant spraying port, a sampling gas collecting port, a gas sampling port, an observation window, an airtight zipper, a pressure gauge, an air pump, an inflatable bottom frame and an air inlet air valve.
Further, the aerosol is prepared from white staphylococcus suspension.
Further, the specified length of time of rest for the contamination operation was 5 minutes.
Further, the sampling includes: connecting a sampling pipe of the air microorganism sampler to a gas sampling port of the sampling air bag by using a hose, connecting an air outlet of an air pump to a sampling gas collecting port of the sampling air bag, and starting a sampling device to sample for 5-10 min.
Further, the terminal sterilization comprises: and starting the air pump to inflate the bacteria-contaminated air bag, then opening an air outlet air valve of the bacteria-contaminated air bag and an air inlet air valve of the sampling air bag, and filling a disinfection substance into the air bag through a disinfection substance injection port to perform final disinfection.
Further, the sterilizing substance is ozone or hydrogen peroxide aerosol.
According to the method for detecting the one-time sterilization rate of the air sterilization equipment, the bacteria-infected air bag and the sampling air bag are respectively connected to the air inlet and the air outlet of the equipment to be detected, so that the sterilized air and the non-sterilized air are separated independently, the one-time sterilization rate of the equipment to be detected can be obtained, and the sterilization capability of the equipment to be detected can be visually reflected. The invention is based on the principle that by measuring the content of test bacteria in the air before and after sterilization of a sterilization device, the one-time sterilization rate can be accurately calculated, in particular suitable for measuring the sterilization rate of pathogenic microorganisms mediated by aerosols. This is because, compared with the circular detection method, the detection of the disposable sterilization rate can reflect the instant sterilization performance of the air sterilization product, and meanwhile, because the test process simulates the real propagation process of pathogenic microorganisms such as new coronavirus taking aerosol as the medium, the detection result is closer to the actual situation.
Drawings
To further clarify the above and other advantages and features of embodiments of the present invention, a more particular description of embodiments of the present invention will be rendered by reference to the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. In the drawings, the same or corresponding parts will be denoted by the same or similar reference numerals for clarity.
FIG. 1 shows a flow diagram of a method for detecting a one-time sterilization rate of an air sanitizer according to an embodiment of the present invention; and
fig. 2 is a schematic structural diagram illustrating an apparatus for detecting a disposable sterilization rate of an air sterilizer according to an embodiment of the present invention.
Detailed Description
In the following description, the present invention is described with reference to examples. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of the specific details, or with other alternative and/or additional methods, materials, or components. In other instances, well-known structures, materials, or operations are not shown or described in detail to avoid obscuring aspects of the invention. Similarly, for purposes of explanation, specific numbers, materials and configurations are set forth in order to provide a thorough understanding of the embodiments of the invention. However, the invention is not limited to these specific details. Further, it should be understood that the embodiments shown in the figures are illustrative representations and are not necessarily drawn to scale.
Reference in the specification to "one embodiment" or "the embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment.
It should be noted that the embodiment of the present invention describes the process steps in a specific order, however, this is only for the purpose of illustrating the specific embodiment, and does not limit the sequence of the steps. Rather, in various embodiments of the present invention, the order of the steps may be adjusted according to process adjustments.
In order to detect the disposable sterilization rate of the air sterilization equipment, the invention provides a method for detecting the disposable sterilization rate of the air sterilization equipment, and the scheme of the invention is further described below by combining the drawings of the embodiment.
Fig. 1 shows a schematic flow diagram of a method for detecting the one-time sterilization rate of an air sanitizer according to an embodiment of the present invention. As shown, a method for detecting a single-use sterilization rate of an air sterilizer apparatus, comprising:
first, in step 101, a device is connected. Connecting an air inlet of the equipment to be detected to the bacteria-contaminated air bag, and connecting an air outlet of the equipment to be detected to the sampling air bag; in one embodiment of the invention, the air inlet of the device to be detected is connected to the air outlet air valve of the contamination air bag through an air pipe, the air outlet air valve is closed, and the air outlet of the device to be detected is connected to the air inlet air valve of the sampling air bag through an air pipe, and the air inlet air valve is in a closed state;
next, in step 102, a contamination operation is performed. Firstly, filling a preset amount of air into the bacteria-contaminated air bag, and then spraying a preset amount of aerosol to enable the number of bacterial colonies in the air in the bacteria-contaminated air bag to reach a preset value, standing for a specified time, wherein the filling amount of the air can be obtained by indicating the number through a pressure meter; in one embodiment of the invention, the resting period is 5 minutes; in another embodiment of the invention, the aerosol is made of white staphylococcus suspension and is sprayed into the bacterial infection air bag through a bacterial spraying port of the bacterial infection air bag;
next, in step 103, a sterilization operation is performed. Opening an air outlet air valve of the bacteria-contaminated air bag and an air inlet air valve of the sampling air bag, and starting the equipment to be detected, so that air in the bacteria-contaminated air bag enters the sampling air bag after being disinfected by the equipment to be detected;
next, at step 104, a sterilization rate is sampled and calculated. After all the gas in the bacteria-contaminated air bag enters the sampling air bag, closing the equipment to be detected, an air outlet air valve of the bacteria-contaminated air bag and an air inlet air valve of the sampling air bag, then sampling the air in the sampling air bag, calculating the colony number of the sampled air, and further obtaining the one-time sterilization rate of the equipment to be detected; in one embodiment of the invention, the sampling comprises: connecting a sampling pipe of the air microorganism sampler to a gas sampling port of the sampling air bag by using a hose, connecting an air outlet of an air pump to a sampling gas collecting port of the sampling air bag, and starting a sampling device to sample for 5-10 min; and
finally, in step 105, terminal sterilization. And (3) sterilizing the device to be detected and/or the bacteria-infecting air bag and/or the sampling air bag. In one embodiment of the invention, the terminal sterilization comprises: and starting the air pump to inflate the bacteria-contaminated air bag, then opening an air outlet air valve of the bacteria-contaminated air bag and an air inlet air valve of the sampling air bag, and filling a disinfection substance into the air bag through a disinfection substance injection port to perform final disinfection. In yet another embodiment of the invention, the disinfecting substance is ozone or hydrogen peroxide aerosol.
The apparatus used in the embodiment of the present invention is shown in fig. 2, and includes a contamination balloon 201 and a sampling balloon 202.
The fungus-infecting air bag 201 comprises a fungus spraying port 2011, a disinfectant spraying port 2012, a viewing window 2013, an airtight zipper 2014, a pressure gauge 2015, an air pump 2016, an air outlet air valve 2017 and an air-inflating bottom frame 2018. The fungus staining gasbag 201 is made for the PVC material, spout fungus mouth 2011, the mouth 2012 of spouting of disinfection material, observation window 2013, airtight zip fastener 2014, manometer 2015, air pump 2016, air outlet blast gate 2017 and aerify and put down 2018 and arrange through the welded mode on the fungus staining gasbag 201, wherein, spout fungus mouth 2011 and be used for spouting into the aerosol, the mouth 2012 of spouting of disinfection material is used for spouting the disinfection material when terminal disinfection, observation window 2013 is used for observing the progress in real time and discover unusually in the testing process in time, airtight zip fastener 2014 is used for the staff to get into the inside equipment maintenance that carries out of fungus staining gasbag, manometer 2015 is used for monitoring fills into the air quantity in the fungus staining gasbag, air pump 2016 is used for aerifing the fungus staining gasbag, air outlet blast gate 2017 is connected to wait to examine equipment 203 for the control gas inflow wait to examine equipment, and the inflatable bottom frame 2018 is used for supporting the bacteria-infected air bag, and the inflatable bottom frame 2018 is inflated through the air pump 2016.
The sampling airbag 202 includes a sterilizing material spraying port 2021, a sampling gas collecting port 2022, a gas sampling port 2023, a viewing window 2024, a gas-tight zipper 2025, a pressure gauge 2026, an air pump 2027, an inflatable bottom frame 2028, and an air inlet/outlet port valve 2029. The sampling air bag 202 is made of PVC material, the sterilizing substance injection port 2021, the sampling gas collection port 2022, the gas sampling port 2023, the observation window 2024, the airtight zipper 2025, the pressure gauge 2026, the air pump 2027, the inflatable bottom frame 2028 and the air inlet/outlet air valve 2029 are arranged on the sampling air bag 202 by welding, wherein the sterilizing substance injection port 2021 is used for injecting sterilizing substance at the time of terminal sterilization, the sampling gas collection port 2022 is used for connecting with the air outlet of the air pump to match with a sampling device to complete sampling at the time of sampling, the gas sampling port 2023 is used for connecting with an air microorganism sampler to realize sampling at the time of sampling, the observation window 2024 is used for observing the progress and finding abnormality at the time in real time during detection, the airtight zipper 2025 is used for workers to enter the inside of the sampling air bag for equipment maintenance, the pressure gauge 2026 is used for monitoring the air quantity in the sampling air bag, the air pump 2027 is used for inflating the inflatable bottom frame 2028, the inflatable bottom frame 2028 is used for supporting the sampling air bag, and the air inlet air valve 2029 is used for controlling air to flow into the sampling air bag from the air outlet of the device to be detected.
According to the method for detecting the one-time sterilization rate of the air sterilization equipment, the bacteria-infecting air bag and the sampling air bag are respectively connected with the air inlet and the air outlet of the equipment to be detected, so that the sterilized air and the non-sterilized air are separated independently, the one-time sterilization rate of the equipment to be detected can be obtained, and the sterilization capability of the equipment to be detected can be visually reflected.
While various embodiments of the present invention have been described above, it should be understood that they have been presented by way of example only, and not limitation. It will be apparent to persons skilled in the relevant art that various combinations, modifications, and changes can be made thereto without departing from the spirit and scope of the invention. Thus, the breadth and scope of the present invention should not be limited by any of the above-described exemplary embodiments, but should be defined only in accordance with the following claims and their equivalents.
Claims (10)
1. A method for detecting the single-use sterilization rate of an air sterilizer, comprising the steps of:
connecting an air inlet of the equipment to be detected to an air outlet valve of the bacteria-staining air bag, connecting an air outlet of the equipment to be detected to an air inlet valve of the sampling air bag, and enabling the air outlet valve of the bacteria-staining air bag and the air inlet valve of the sampling air bag to be in a closed state;
filling a preset amount of air into the bacteria-infected air bag, then spraying a preset amount of aerosol, and standing for a specified time;
opening an air outlet valve of the bacteria-contaminated air bag and an air inlet valve of the sampling air bag, and starting the equipment to be detected, so that air in the bacteria-contaminated air bag enters the sampling air bag after being disinfected by the equipment to be detected; and
and after all the gas in the bacteria-contaminated air bag enters the sampling air bag, closing the equipment to be detected, an air outlet air valve of the bacteria-contaminated air bag and an air inlet air valve of the sampling air bag, sampling the air in the sampling air bag, calculating the aerosol content of the sampled air, and further obtaining the one-time sterilization rate of the equipment to be detected.
2. The method of claim 1, further comprising the step of:
and sterilizing the equipment to be detected, the bacteria-contaminated air bag and/or the sampling air bag.
3. The method of claim 1, wherein said infectious microbe bladder comprises a germ spray port, a sterilizing substance spray port, a pressure gauge, an air pump, and an air outlet damper.
4. The method as claimed in claim 3, wherein the air inlet of the device to be tested is connected to the air outlet air valve of the contamination airbag, the air outlet air valve is closed during the contamination operation, and is opened during the sterilization operation, and is closed again after the sterilization operation is completed.
5. The method of claim 3, wherein said infectious air bag further comprises a viewing window, an airtight zipper, and an inflatable bottom frame.
6. The method of claim 3, wherein the sampling bladder comprises a sanitizing substance spray inlet, a sampling gas collection port, a gas sampling port, a sight glass, a gas tight zipper, a pressure gauge, a gas pump, an inflatable base frame, an air inlet vent valve.
7. The method of claim 1, wherein said aerosol is made of a suspension of staphylococcus albus.
8. The method of claim 3, wherein the specified length of time of rest for the microbiological contamination is 5 minutes.
9. The method of claim 6, wherein the sampling comprises: connecting a sampling pipe of the air microorganism sampler to a gas sampling port of the sampling air bag by using a hose, connecting an air outlet of an air pump to a sampling gas collecting port of the sampling air bag, and starting a sampling device to sample for 5-10 min.
10. The method of claim 6, wherein the sterilizing comprises: and starting an air pump to inflate the bacteria-infected air bag, then opening an air outlet air valve of the bacteria-infected air bag and an air inlet air valve of the sampling air bag, filling a disinfection substance into the air bag through a disinfection substance injection port, and finally disinfecting.
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| CN202011177652.4A CN112391439B (en) | 2020-10-29 | 2020-10-29 | Method for detecting disposable sterilization rate of air disinfection equipment |
| CN202211010283.9A CN115323030A (en) | 2020-10-29 | 2020-10-29 | A device for detecting the one-time sterilization rate of air disinfection equipment |
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| CN202011177652.4A CN112391439B (en) | 2020-10-29 | 2020-10-29 | Method for detecting disposable sterilization rate of air disinfection equipment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CA1231295A (en) * | 1984-05-22 | 1988-01-12 | Thomas A. Augurt | Disposable prevacuum steam sterilizer test device |
| AU647041B2 (en) * | 1989-09-22 | 1994-03-17 | Minnesota Mining And Manufacturing Company | Disposable test packs for steam or gas sterilizers |
| ES2183813T3 (en) * | 1992-05-05 | 2003-04-01 | Colvin Jane E | APPARATUS AND TEST METHOD OF A STERILIZER. |
| CA2125810A1 (en) * | 1993-07-01 | 1995-01-02 | Jarrell D. Wenger | Apparatus and method for determining the indoor air quality within an enclosed space |
| AU2003220757A1 (en) * | 2002-04-05 | 2003-10-20 | Sharp Kabushiki Kaisha | Method of estimating elimination of microorganisms and apparatus for estimating elimination of microorganisms |
| CN101144749B (en) * | 2007-10-09 | 2010-08-18 | 中国建筑科学研究院建筑环境与节能研究院 | Multifunctional air purifying disinfection device performance detection system |
| CN103805499B (en) * | 2014-01-20 | 2016-05-18 | 北京工业大学 | Air sterilization effect pipeline evaluation system |
| CN205506783U (en) * | 2015-10-16 | 2016-08-24 | 天津城建大学 | Air contaminant experimental apparatus |
| WO2019145525A1 (en) * | 2018-01-29 | 2019-08-01 | Confarma France | System for exposure to a product in the form of an aerosol and method for evaluating the inegrity of a container by means of such a system |
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