CN112391285B - Continuous nucleic acid detection reactor and use method thereof - Google Patents
Continuous nucleic acid detection reactor and use method thereof Download PDFInfo
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- CN112391285B CN112391285B CN202011568042.7A CN202011568042A CN112391285B CN 112391285 B CN112391285 B CN 112391285B CN 202011568042 A CN202011568042 A CN 202011568042A CN 112391285 B CN112391285 B CN 112391285B
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 30
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 30
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000001514 detection method Methods 0.000 title abstract description 13
- 238000002347 injection Methods 0.000 claims abstract description 49
- 239000007924 injection Substances 0.000 claims abstract description 49
- 238000001179 sorption measurement Methods 0.000 claims abstract description 46
- 238000012360 testing method Methods 0.000 claims abstract description 42
- 239000006166 lysate Substances 0.000 claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims abstract description 27
- 238000001125 extrusion Methods 0.000 claims abstract description 25
- 238000004891 communication Methods 0.000 claims abstract description 7
- 239000011324 bead Substances 0.000 claims description 39
- 230000007246 mechanism Effects 0.000 claims description 30
- 238000003860 storage Methods 0.000 claims description 29
- 230000000694 effects Effects 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 10
- 230000003068 static effect Effects 0.000 claims description 10
- 230000001502 supplementing effect Effects 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 7
- 230000037431 insertion Effects 0.000 claims description 7
- 238000012423 maintenance Methods 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000004804 winding Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000009434 installation Methods 0.000 description 11
- 241000700605 Viruses Species 0.000 description 6
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
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- 241000711573 Coronaviridae Species 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
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- 238000001802 infusion Methods 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
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- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention discloses a continuous nucleic acid detection reactor and a using method thereof, wherein the device comprises a reaction table, a quantitative box, an adsorption box and a conveyor belt, the conveyor belt is horizontally and fixedly arranged at the upper end of the reaction table, a plurality of placing grooves are uniformly formed in the front end of the conveyor belt, a hole groove is formed in the center of the inside of each placing groove, a test tube box is arranged in the inside of each placing groove, and the quantitative box is fixedly arranged at the left side of the upper end of the reaction table. According to the invention, when the first electric push rod moves downwards, the extrusion plate is driven to move downwards, so that the lysate in the quantitative box is extruded, the lysate breaks through the rubber valve, then the lysate is injected into a test tube in the test tube box through the injection tube, the pressure at the lower end of the extrusion plate is reduced after injection, the rubber valve is reset to seal the hollow tube, and the communication time of the first contact and the second contact is the same each time, so that the injection amount is the same each time, the quantitative injection is convenient, the injection work does not need to be carried out manually, and the injection is rapid and convenient.
Description
Technical Field
The invention relates to the field of medical equipment, in particular to a continuous nucleic acid detection reactor and a using method thereof.
Background
The substance for nucleic acid detection is a nucleic acid of a virus. The nucleic acid detection is to find out whether the respiratory tract specimen, blood or feces of the patient have the nucleic acid of the virus invaded from the outside to determine whether the patient is infected by the new coronavirus. Thus, once a nucleic acid is detected as "positive", the presence of the virus in the patient is demonstrated.
After the new coronavirus infects human body, it will propagate in respiratory tract system, so it can judge whether human body is infected by virus by detecting virus nucleic acid in sputum and nasopharyngeal swab. Therefore, the positive detection of nucleic acid can be used as the standard for the accurate diagnosis of the novel coronavirus infection.
The existing nucleic acid inspection needs to extract nucleic acid in a reactor and then carry out detection work, and the existing reactor has the following defects when in use:
1. the existing reactor needs to manually inject the lysis solution into the test tube quantitatively during reaction, the manual injection control amount is accurate, but the injection is time-consuming, the detection reaction efficiency is reduced, and the use is inconvenient;
2. when the existing reactor is used, the system control is generally carried out through a control box to control the whole process, and once bug occurs in the system control during the use, the system needs to be adjusted and repaired, so that the time is extremely consumed for repairing the unusual bug, the maintenance period of the whole equipment is too long, and the use of the equipment is influenced;
3. the existing reactor needs to suck out magnetic beads inside a test tube through a magnetic rod after virus cracking so as to obtain pure nucleic acid, the magnetic rod needs to be sterilized by independent equipment after being used, the magnetic beads are also inconvenient to collect in a centralized manner, and the magnetic beads need to be collected independently every time, so that the problem is solved urgently by a device.
Disclosure of Invention
The present invention is directed to a continuous nucleic acid detecting reactor and a method for using the same, which solve the above problems of the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a continuous nucleic acid detection reactor comprises a reaction table, a quantitative box, an adsorption box and a conveyor belt, wherein the conveyor belt is horizontally and fixedly arranged at the upper end of the reaction table, a plurality of placing grooves are uniformly formed in the front end of the conveyor belt, a hole groove is formed in the central position inside the placing grooves, a test tube box is arranged in the placing grooves, test tubes are conveniently placed in the test tube box, the left side of the upper end of the reaction table is fixedly provided with the quantitative box, lysate is conveniently stored in the quantitative box, a first electric push rod is vertically and fixedly arranged at the left end of the quantitative box, a control switch is fixedly arranged at the left end of the first electric push rod at the upper end of the reaction table and is convenient for controlling the extension of the first electric push rod, a storage mechanism is arranged at the right end of the quantitative box, and an extension plate is horizontally and integrally formed at the front end of the left side of the conveyor belt, the device is characterized in that a gear is movably arranged at the left side position of the front end of the extension plate, a rotating shaft is movably and alternately arranged at the center position of the front end of the extension plate, a half gear is fixedly arranged at the lower end of the rotating shaft and meshed with the gear, a disc is fixedly welded at the lower end of the half gear, a conducting block is fixedly embedded at the left end of the disc, a baffle is fixedly welded at the front end of the extension plate, the right end of the disc is tightly attached to the left side of the baffle, a first contact is fixedly embedded at the left end of the baffle, a second contact is fixedly embedded at the lower end of the first contact at the left end of the baffle, a motor is fixedly arranged at the upper end of the extension plate through a mounting frame, the output end position of the lower end of the motor is fixedly arranged at the upper end of the rotating shaft through a shaft coupling, and the motor drives the rotating shaft to rotate, so that the half gear rotates, when the half gear rotates to a position meshed with the gear, the conveyor belt is driven to move for a certain distance until the half gear is separated from the gear, the conveyor belt is in a static state, then when the conductive block rotates to simultaneously contact the first contact and the second contact, the first contact is communicated with the second contact, the first electric push rod is driven to move downwards, when the conductive block moves to a position which is not simultaneously contacted with the first contact and the second contact, the first electric push rod is in a static state, an L-shaped rod is fixedly welded at the upper end of the first electric push rod, the lower end of the L-shaped rod movably penetrates into the inner position of the quantitative box, an extrusion plate is movably clamped at the inner position of the quantitative box, a through hole is formed at the right end of the extrusion plate, a rubber ring is fixedly bonded at the central position of the inner part of the through hole horizontally, and the lower end of the L-shaped rod is fixedly welded at the central position of the upper end of the extrusion plate, the reaction table is vertically and fixedly inserted with a hollow pipe, the hollow pipe is fixedly bonded and fixedly provided with a rubber valve, the protruding end of the rubber valve is downward, the lower end of the hollow pipe is fixedly welded with an injection pipe, when the conveyor belt stops, the test tube box is just positioned under the injection pipe, the extrusion plate is driven to move downward until the first electric push rod moves downward, so that lysate in the quantitative box is extruded, the lysate breaks through the rubber valve, then the lysate is injected into a test tube in the test tube box through the injection pipe, the pressure at the lower end of the extrusion plate is reduced after injection, the rubber valve is reset to seal the hollow pipe, the communication time of the first contact and the second contact is the same each time, the injection amount is the same each time, the quantitative injection is convenient, the injection work is not needed manually, the injection is rapid and convenient, the support plate is horizontally welded and fixedly arranged at the lower end of the reaction table, the upper end of the supporting plate is vertically and fixedly provided with a second electric push rod, the upper end of the second electric push rod is horizontally and fixedly welded with a lifting plate, the central position in the adsorption box is movably clamped with a magnetic plate, the magnetic plate slides up and down along the position in the adsorption box, the position in the adsorption box is provided with a disinfection mechanism, the position in the adsorption box is uniformly and vertically and fixedly provided with a plurality of magnetic rods, the magnetic rods movably penetrate through the magnetic plate, the right side position of the second electric push rod is fixedly welded with a connecting rod, the connecting rod movably penetrates into the position in the adsorption box and is fixedly arranged at the upper end of the magnetic plate, when the conveyor belt stops, the second electric push rod moves upwards to drive the lifting plate to move upwards, so that the lifting plate passes through the central position of the hole groove and then moves into the adsorption box, and the connecting rod drives the magnetic plate to move upwards, until the bar magnet penetrates the inside test tube central point of test tube box and puts, then make the magnetic bead adsorb in bar magnet outside position, later the second electric putter resets, drives the magnetic plate downstream through the connecting rod, scrapes the magnetic bead in the bar magnet outside to make the magnetic bead adsorb in magnetic plate lower extreme position, make things convenient for the concentrated collection of magnetic bead, adsorption box right side end position has seted up the draw-in groove, draw-in groove inside position bonds fixedly and is provided with sealed the pad, draw-in groove inside position activity joint is provided with the closing plate, the clearance groove has been seted up to adsorption box right side end position, adsorption box right side end position is provided with fixed establishment.
Preferably, the storage mechanism comprises a check valve, a connecting pipe, a liquid supplementing pipe, a cover plate and a storage box, the right side of the quantitative box is fixedly provided with the storage box at the upper end of the reaction table, the internal position of the storage box is convenient for storing the lysate, the central position at the upper end of the storage box is vertically and fixedly provided with the liquid supplementing pipe in an inserting way, the upper end of the liquid supplementing pipe is provided with the cover plate in a winding way through threads, the upper end of the cover plate is provided with a plurality of air inlets, the front end of the storage box is fixedly provided with a transparent observation plate in an embedding way, the transparent observation plate is convenient for observing the storage condition in the storage box, the internal position of the storage box is vertically and fixedly and alternately provided with the connecting pipe, the left end of the connecting pipe is fixedly inserted into the internal position of the quantitative box and movably penetrates through the central position of the rubber ring, the outer diameter of the connecting pipe is larger than the inner diameter of the rubber ring, when the lysate in the quantitative box needs to be supplemented, the user passes through control switch, and control first electric putter shifts up and resets, and the quantitative incasement portion forms the negative pressure this moment to inhale quantitative incasement portion position through the connecting pipe with the inside lysate of bin, thereby make things convenient for the fluid infusion.
Preferably, fixed establishment includes screw rod, loose ring, restriction piece and limiting plate, it is provided with the screw rod to adsorb case right side end position level welded fastening, the activity of screw rod outside end position cup joints and is provided with the limiting plate, screw rod right side end position welded fastening is provided with the restriction piece, screw rod outside end position is provided with the loose ring through the screw thread wraparound, and the loose ring is controlled along the screw rod through rotating, and the user makes the loose ring remove to the left side along the screw rod through rotating the loose ring, extrudees the limiting plate and removes to the left side to extrude fixed closing plate, when needing to handle the magnetic bead, user's antiport loose ring, then can dismantle the closing plate to the right side, later can collect the work to the magnetic bead through the clearance groove.
Preferably, disinfection mechanism includes lamp shade, ultraviolet lamp, protrusion piece, trigger switch and installation mechanism, the inside position of adsorption tank is provided with the lamp shade, the inside fixed position installation of lamp shade is provided with the ultraviolet lamp, the inside both sides position welded fastening of adsorption tank is provided with the protrusion piece, protrusion piece upper end position fixed mounting is provided with trigger switch, lamp shade both sides position is provided with installation mechanism.
Preferably, installation mechanism includes a movable section of thick bamboo, third contact, inserted block, spring and rectangular block, the ultraviolet lamp both sides are provided with the inserted block at the inside fixed position installation of adsorption box, the equal activity of lamp shade both sides position end position is cup jointed and is provided with a movable section of thick bamboo, movable section of thick bamboo outside end position welded fastening is provided with the rectangular block, rectangular block outside end position fixed mounting is provided with the third contact, the third contact agrees with the inserted block, fixed position is connected between the rectangle inboard and the lamp shade outside end of block and is provided with the spring, and when the ultraviolet lamp needs to be dismantled, the user removes a movable section of thick bamboo along the lamp shade to make the spring extruded, break away from the inserted block until the third contact, can take off the ultraviolet lamp and change.
Preferably, the gear is coaxial with the conveyor belt.
Preferably, the lifting plate is smaller than the hole groove.
Preferably, the first contact, the second contact and the first electric push rod are electrically connected, and an open circuit is formed between the first contact and the second contact.
A use method of a continuous nucleic acid detection reactor specifically comprises the following steps:
the first step is as follows: the test tube box is arranged at the inner position of the arranging groove, then the motor is started, the motor drives the rotating shaft to rotate, so that the half gear rotates, the conveying belt is driven to move for a certain distance when the half gear rotates to a position meshed with the gear until the half gear is separated from the gear, the conveying belt is in a static state at the moment, then when the conducting block rotates to simultaneously contact the first contact and the second contact, the first contact is communicated with the second contact, the first electric push rod is driven to move downwards, and when the conducting block moves to a position where the conducting block does not simultaneously contact the first contact and the second contact, the first electric push rod is in a static state;
the second step is that: when the first electric push rod moves downwards, the extrusion plate is driven to move downwards, so that the lysate in the quantitative box is extruded, the lysate breaks through the rubber valve, the lysate is injected into a test tube in the test tube box through the injection tube, the pressure at the lower end of the extrusion plate is reduced after the lysate is injected, the rubber valve is reset to seal the hollow tube, the communication time of the first contact and the second contact is the same each time, the injection amount is the same each time, the quantitative injection is convenient, the manual injection work is not needed, and the injection is quick and convenient;
the third step: when the conveyor belt stops, the second electric push rod moves upwards to drive the lifting plate to move upwards, so that the lifting plate penetrates through the center position of the hole groove and then moves to the inside of the adsorption box, the connecting rod drives the magnetic plate to move upwards until the magnetic rod penetrates into the center position of a test tube in the interior of the test tube box, then the magnetic beads are adsorbed at the outer side position of the magnetic rod, the second electric push rod resets, the magnetic plate is driven to move downwards through the connecting rod, the magnetic beads at the outer side of the magnetic rod are scraped, the magnetic beads are adsorbed at the lower end position of the magnetic plate, the concentrated collection of the magnetic beads is facilitated, the whole equipment does not need to be controlled by a control system such as a control box and the like, and the stability is high, and the maintenance work is facilitated;
the fourth step: when the connecting rod moves downwards, the magnetic plate is driven to move downwards, so that the magnetic plate extrudes the trigger switch, the ultraviolet lamp is started, and the magnetic bar is irradiated, sterilized and disinfected, so that the magnetic bar is kept in a clean state when being reused;
the fifth step: the user makes the activity ring remove to the left side along the screw rod through rotating the activity ring, extrudees the limiting plate and removes to the left side to extrude fixed seal board, when needing to handle the magnetic bead, the user reverse rotation activity ring, then can dismantle the closing plate to the right side, later can collect the work to the magnetic bead through the clearance groove.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, when the first electric push rod moves downwards, the extrusion plate is driven to move downwards, so that the lysate in the quantitative box is extruded, the lysate breaks through the rubber valve, then the lysate is injected into a test tube in the test tube box through the injection tube, the pressure at the lower end of the extrusion plate is reduced after injection, the rubber valve is reset to seal the hollow tube, and the communication time of the first contact and the second contact is the same each time, so that the injection amount is the same each time, the quantitative injection is convenient, the manual injection work is not needed, and the injection is rapid and convenient;
2. according to the invention, a user rotates the movable ring to enable the movable ring to move to the left side along the screw rod, the extrusion limiting plate moves to the left side, so that the fixed sealing plate is extruded, when magnetic beads need to be processed, the user rotates the movable ring reversely, then the sealing plate can be disassembled to the right side, and then the magnetic beads can be collected through the cleaning groove;
3. according to the invention, the second electric push rod moves upwards to drive the lifting plate to move upwards, so that the lifting plate passes through the center position of the hole groove and then moves to the inside of the adsorption box, the connecting rod drives the magnetic plate to move upwards until the magnetic rod penetrates through the center position of the test tube in the interior of the test tube box, then the magnetic beads are adsorbed at the outer side position of the magnetic rod, then the second electric push rod resets, the connecting rod drives the magnetic plate to move downwards to scrape the magnetic beads at the outer side of the magnetic rod and adsorb the magnetic beads at the lower end position of the magnetic plate, so that the concentrated collection of the magnetic beads is convenient, the whole equipment does not need a control system such as a control box to control the work, and the stability is high, and the maintenance work is convenient.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a continuous nucleic acid detecting reactor according to the present invention;
FIG. 2 is a sectional view of a quantitative tank in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 3 is a schematic diagram of the structure of A1 in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 4 is a schematic diagram of a fixing mechanism in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 5 is a top view of a conveyor belt in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 6 is a top view of a gear in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 7 is a schematic view of the first contact mounting in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 8 is a left sectional view of an adsorption tank in a continuous nucleic acid detecting reactor according to the present invention;
FIG. 9 is a schematic diagram of the structure of the installation mechanism in the continuous nucleic acid detecting reactor according to the present invention.
In the figure: 1. a reaction platform; 2. a conveyor belt; 3. a test tube box; 4. a placing groove; 5. a hole groove; 6. a gear; 7. a rotating shaft; 8. a half gear; 9. a disc; 10. a baffle plate; 11. an extension plate; 12. a conductive block; 13. a first contact; 14. a first electric push rod; 15. a control switch; 16. a support plate; 17. a second electric push rod; 18. lifting the plate; 19. a connecting rod; 20. a sealing plate; 21. an adsorption tank; 22. a quantitative box; 23. a storage tank; 24. a transparent viewing plate; 25. a liquid supplementing pipe; 26. a connecting pipe; 27. a one-way valve; 28. an ultraviolet lamp; 29. a lamp shade; 30. a protruding block; 31. a trigger switch; 32. an L-shaped rod; 33. a pressing plate; 34. a cover plate; 35. perforating; 36. a rubber ring; 37. a hollow tube; 38. an injection pipe; 39. a rubber valve; 40. a magnetic plate; 41. a magnetic bar; 42. a second contact; 43. a card slot; 44. a gasket; 45. cleaning the tank; 46. a screw; 47. a motor; 48. a limiting block; 49. a movable ring; 50. a limiting plate; 51. a movable barrel; 52. inserting a block; 53. a third contact; 54. a rectangular block; 55. a spring; 56. a sterilizing mechanism; 57. an installation mechanism; 58. a storage mechanism; 59. and a fixing mechanism.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-9, the present invention provides a technical solution: a continuous nucleic acid detection reactor comprises a reaction table 1, a quantitative box 22, an adsorption box 21 and a conveyor belt 2, wherein the conveyor belt 2 is horizontally and fixedly arranged at the upper end position of the reaction table 1, a plurality of placing grooves 4 are uniformly formed in the front end position of the conveyor belt 2, a hole groove 5 is formed in the central position inside the placing groove 4, a test tube box 3 is placed and arranged at the inner position of the placing groove 4, a test tube is conveniently placed and arranged at the inner position of the test tube box 3, the quantitative box 22 is fixedly arranged at the left end position of the upper end of the reaction table 1, lysate is conveniently stored inside the quantitative box 22, a first electric push rod 14 is vertically and fixedly arranged at the left end position of the quantitative box 22, a control switch 15 is fixedly arranged at the left end position of the first electric push rod 14 at the upper end of the reaction table 1, the control switch 15 is convenient for controlling the extension of the first electric push rod 14, a storage mechanism 58 is arranged at the right end position of the quantitative box 22, 2 left side front end position level integrated into one piece of conveyer belt is provided with extension board 11, 11 front end left side position movable mounting of extension board is provided with gear 6, gear 6 is coaxial with conveyer belt 2, 11 front end central point of extension board puts the activity and alternates and is provided with pivot 7, 7 lower extreme position fixed mounting of pivot is provided with half gear 8, half gear 8 and the meshing of gear 6, 8 lower extreme position welded fastening of half gear is provided with disc 9, 9 left side end position of disc inlays fixedly and is provided with conducting block 12, 11 front end position welded fastening of extension board is provided with baffle 10, 9 right side end position of disc hugs closely in the baffle 10 left side, baffle 10 left side end position inlays fixedly and is provided with first contact 13, 13 lower extreme is inlayed fixedly and is provided with second contact 42 in baffle 10 left side end position, first contact 13, The second contact 42 is electrically connected with the first electric push rod 14, and an open circuit is formed between the first contact 13 and the second contact 42, the upper end position of the extension plate 11 is fixedly provided with a motor 47 through a mounting frame, the lower end output end position of the motor 47 is fixedly arranged at the upper end position of the rotating shaft 7 through a coupling, the motor 47 drives the rotating shaft 7 to rotate, so that the half gear 8 rotates, when the half gear 8 rotates to a position meshed with the gear 6, the conveyor belt 2 is driven to move for a certain distance until the half gear 8 is separated from the gear 6, at the moment, the conveyor belt 2 is in a static state, then when the conductive block 12 rotates to simultaneously contact the first contact 13 and the second contact 42, the first contact 13 is communicated with the second contact 42, the first electric push rod 14 is driven to move downwards, until the conductive block 12 moves to a position which does not simultaneously contact the first contact 13 and the second contact 42, the first electric push rod 14 is in a static state, an L-shaped rod 32 is fixedly welded at the upper end position of the first electric push rod 14, the lower end position of the L-shaped rod 32 movably penetrates into the inner position of the quantitative box 22, an extrusion plate 33 is movably clamped at the inner position of the quantitative box 22, a through hole 35 is formed in the right end position of the extrusion plate 33, a rubber ring 36 is fixedly and horizontally adhered at the inner center position of the through hole 35, the lower end position of the L-shaped rod 32 is fixedly welded at the upper end center position of the extrusion plate 33, a hollow tube 37 is vertically and fixedly penetrated at the inner position of the reaction table 1, a rubber valve 39 is fixedly and fixedly adhered at the inner position of the hollow tube 37, the protruding end of the rubber valve 39 faces downwards, an injection tube 38 is fixedly welded at the lower end position of the hollow tube 37, and when the conveyor belt 2 stops, the test tube box 3 is just positioned under the injection tube 38, until the first electric push rod 14 moves downwards, the extrusion plate 33 is driven to move downwards, so that the lysate in the quantitative box 22 is extruded, the lysate breaks through the rubber valve 39, then the lysate is injected into a test tube in the test tube box 3 through the injection tube 38, the pressure at the lower end of the extrusion plate 33 is reduced after injection, the rubber valve 39 is reset to seal the hollow tube 37, the communication time of the first contact 13 and the second contact 42 is the same each time, the injection amount is the same each time, quantitative injection is convenient, manual injection work is not needed, the injection is rapid and convenient, the support plate 16 is horizontally and fixedly welded at the lower end of the reaction table 1, the second electric push rod 17 is vertically and fixedly installed at the upper end of the support plate 16, the lifting plate 18 is horizontally and fixedly welded at the upper end of the second electric push rod 17, the lifting plate 18 is smaller than the hole groove 5, the magnetic plate 40 is movably clamped at the central position in the adsorption box 21, the magnetic plate 40 slides up and down along the inner position of the adsorption box 21, the disinfection mechanism 56 is arranged at the inner position of the adsorption box 21, a plurality of magnetic rods 41 are uniformly and vertically fixedly arranged at the inner position of the adsorption box 21, the magnetic rods 41 movably penetrate through the magnetic plate 40, the connecting rod 19 is fixedly welded at the right side end position of the second electric push rod 17, the connecting rod 19 movably penetrates through the inner position of the adsorption box 21 and is fixedly arranged at the upper end of the magnetic plate 40, when the conveyor belt 2 stops, the second electric push rod 17 moves upwards to drive the lifting plate 18 to move upwards, so that the lifting plate 18 passes through the central position of the hole slot 5 and then moves to the inner part of the adsorption box 21, the connecting rod 19 drives the magnetic plate 40 to move upwards until the magnetic rods 41 penetrate through the central position of the test tubes in the test tube box 3, then the magnetic beads are adsorbed at the outer side position of the magnetic rods 41, and then the second electric push rod 17 resets, drive magnetic sheet 40 downstream through connecting rod 19, scrape the magnetic bead in the bar magnet 41 outside to make the magnetic bead adsorb in magnetic sheet 40 lower extreme position, make things convenient for the concentrated collection of magnetic bead, draw-in groove 43 has been seted up to adsorption tank 21 right side end position, draw-in groove 43 internal position bonds fixedly and is provided with sealed pad 44, draw-in groove 43 internal position activity joint is provided with closing plate 20, adsorption tank 21 right side end position has been seted up clearance groove 45, adsorption tank 21 right side end position is provided with fixed establishment 59.
The storage mechanism 58 comprises a check valve 27, a connecting pipe 26, a liquid supplementing pipe 25, a cover plate 34 and a storage box 23, the right side of the quantitative box 22 is fixedly provided with the storage box 23 at the upper end position of the reaction table 1, the internal position of the storage box 23 is convenient for storing lysate, the central position at the upper end of the storage box 23 is vertically penetrated and fixedly provided with the liquid supplementing pipe 25, the upper end position of the liquid supplementing pipe 25 is provided with the cover plate 34 by thread winding, the upper end position of the cover plate 34 is provided with a plurality of air inlets, the front end position of the storage box 23 is fixedly embedded and provided with a transparent observation plate 24, the transparent observation plate 24 is convenient for observing the internal storage condition of the storage box 23, the internal position of the storage box 23 is vertically fixedly penetrated and provided with the connecting pipe 26, the left end position of the connecting pipe 26 is fixedly penetrated into the internal position of the quantitative box 22 and movably penetrates through the central position of the rubber ring 36, the external diameter of the connecting pipe 26 is larger than the internal diameter of the rubber ring 36, when the inside lysate of quantitative case 22 needs to supply, the user passes through control switch 15, and the first electric putter 14 of control moves up and resets, and quantitative case 22 is inside to form the negative pressure this moment to inhale quantitative case 22 internal position with the inside lysate of bin 23 through connecting pipe 26, thereby make things convenient for the fluid infusion.
The fixing mechanism 59 comprises a screw rod 46, a movable ring 49, a limiting block 48 and a limiting plate 50, the screw rod 46 is horizontally and fixedly welded at the right end of the adsorption box 21, the limiting plate 50 is movably sleeved at the outer end of the screw rod 46, the limiting block 48 is fixedly welded at the right end of the screw rod 46, the movable ring 49 is arranged at the outer end of the screw rod 46 in a winding manner through threads, the movable ring 49 moves left and right along the screw rod 46 through rotation, a user enables the movable ring 49 to move left along the screw rod 46 through rotating the movable ring 49, the limiting plate 50 is extruded to move left, and the sealing plate 20 is extruded and fixed, when the magnetic beads need to be processed, the movable ring 49 is rotated reversely by the user, the sealing plate 20 can be disassembled right, and then the magnetic beads can be collected through the cleaning groove 45.
The mounting mechanism 57 includes a movable cylinder 51, a third contact 53, an insertion block 52, a spring 55 and a rectangular block 54, the insertion block 52 is fixedly mounted at the inner position of the adsorption box 21 on both sides of the ultraviolet lamp 28, the movable cylinder 51 is movably sleeved at both end positions of the lamp shade 29, the rectangular block 54 is fixedly welded at the outer end position of the movable cylinder 51, the third contact 53 is fixedly mounted at the outer end position of the rectangular block 54, the third contact 53 is engaged with the insertion block 52, the spring 55 is fixedly connected between the inner side of the rectangular block 54 and the outer end of the lamp shade 29, when the ultraviolet lamp 28 needs to be detached, a user moves the movable cylinder 51 along the lamp shade 29, and the spring 55 is squeezed until the third contact 53 is disengaged from the insertion block 52, so that the ultraviolet lamp 28 can be removed and replaced.
A use method of a continuous nucleic acid detection reactor specifically comprises the following steps:
the first step is as follows: placing the test tube box 3 at an inner position of the placing groove 4, then starting the motor 47, wherein the motor 47 drives the rotating shaft 7 to rotate, so that the half gear 8 rotates, when the half gear 8 rotates to a position meshed with the gear 6, the conveyor belt 2 is driven to move for a certain distance until the half gear 8 is separated from the gear 6, at the moment, the conveyor belt 2 is in a static state, and then when the conductive block 12 rotates to simultaneously contact the first contact 13 and the second contact 42, the first contact 13 is communicated with the second contact 42, the first electric push rod 14 is driven to move downwards, and until the conductive block 12 moves to a position which is not simultaneously contacted with the first contact 13 and the second contact 42, the first electric push rod 14 is in a static state;
the second step is that: the first electric push rod 14 drives the extrusion plate 33 to move downwards when moving downwards, so that the lysate in the quantitative box 22 is extruded, the lysate breaks through the rubber valve 39, the lysate is injected into a test tube in the test tube box 3 through the injection tube 38, the pressure at the lower end of the extrusion plate 33 is reduced after injection, the rubber valve 39 is reset to seal the hollow tube 37, the communication time of the first contact 13 and the second contact 42 is the same each time, the injection amount is the same each time, quantitative injection is convenient, manual injection work is not needed, and the injection is quick and convenient;
the third step: when the conveyor belt 2 stops, the second electric push rod 17 moves upwards to drive the lifting plate 18 to move upwards, so that the lifting plate 18 penetrates through the center position of the hole groove 5 and then moves into the adsorption box 21, the connecting rod 19 drives the magnetic plate 40 to move upwards until the magnetic rod 41 penetrates into the center position of a test tube in the test tube box 3, then the magnetic beads are adsorbed at the outer side position of the magnetic rod 41, then the second electric push rod 17 resets, the connecting rod 19 drives the magnetic plate 40 to move downwards to scrape the magnetic beads at the outer side of the magnetic rod 41 and adsorb the magnetic beads at the lower end position of the magnetic plate 40, the concentrated collection of the magnetic beads is facilitated, the whole equipment does not need to be controlled by a control system such as a control box and the like, the stability is high, and the maintenance work is facilitated;
the fourth step: when the connecting rod 19 moves downwards, the magnetic plate 40 is driven to move downwards, so that the magnetic plate 40 extrudes the trigger switch 31, the ultraviolet lamp 28 is started, the magnetic bar 41 is irradiated, sterilized and disinfected, and the magnetic bar 41 is kept in a clean state when being reused;
the fifth step: the user makes activity ring 49 remove to the left side along screw rod 46 through rotating activity ring 49, extrudees limiting plate 50 and removes to the left side to extrude fixed seal plate 20, when needing to handle the magnetic bead, user's reverse rotation activity ring 49, then can dismantle sealing plate 20 to the right side, later can collect the work to the magnetic bead through clearance groove 45.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (2)
1. A continuity nucleic acid detecting reactor, includes reaction platform (1), ration case (22), adsorption case (21) and conveyer belt (2), its characterized in that: the device is characterized in that a conveyor belt (2) is horizontally and fixedly arranged at the upper end of a reaction table (1), a plurality of placing grooves (4) are uniformly formed in the front end of the conveyor belt (2), a hole groove (5) is formed in the center position of the interior of each placing groove (4), a test tube box (3) is arranged in the interior of each placing groove (4), a quantitative box (22) is fixedly arranged at the left end of the upper end of the reaction table (1), a first electric push rod (14) is vertically and fixedly arranged at the left end of the quantitative box (22), a control switch (15) is fixedly arranged at the left end of the first electric push rod (14) at the upper end of the reaction table (1), a storage mechanism (58) is arranged at the right end of the quantitative box (22), an extension plate (11) is horizontally arranged at the left end of the conveyor belt (2), a gear (6) is movably arranged at the left end of the extension plate (11), the front end center position of the extension plate (11) is movably inserted with a rotating shaft (7), the lower end position of the rotating shaft (7) is fixedly provided with a half gear (8), the half gear (8) is meshed with a gear (6), the lower end position of the half gear (8) is fixedly provided with a disc (9), the left end position of the disc (9) is fixedly provided with a conducting block (12), the front end position of the extension plate (11) is fixedly provided with a baffle (10), the right end position of the disc (9) is tightly attached to the left side of the baffle (10), the left end position of the baffle (10) is fixedly provided with a first contact (13), the lower end of the first contact (13) is fixedly provided with a second contact (42) at the left end position of the baffle (10), the upper end position of the extension plate (11) is fixedly provided with a motor (47), the lower end output end position of the motor (47) is fixedly arranged at the upper end position of the rotating shaft (7), the device is characterized in that an L-shaped rod (32) is fixedly arranged at the upper end of the first electric push rod (14), the lower end of the L-shaped rod (32) is movably inserted into the inner position of the metering box (22), an extrusion plate (33) is movably clamped at the inner position of the metering box (22), a perforation (35) is formed in the right end of the extrusion plate (33), a rubber ring (36) is horizontally and fixedly arranged at the inner central position of the perforation (35), the lower end of the L-shaped rod (32) is fixedly arranged at the upper end central position of the extrusion plate (33), a hollow pipe (37) is vertically and fixedly inserted into the inner position of the reaction table (1), a rubber valve (39) is fixedly arranged at the inner position of the hollow pipe (37), the protruding end of the rubber valve (39) is downward, an injection pipe (38) is fixedly arranged at the lower end of the hollow pipe (37), a support plate (16) is horizontally and fixedly arranged at the lower end of the reaction table (1), the upper end of the supporting plate (16) is vertically and fixedly provided with a second electric push rod (17), the upper end of the second electric push rod (17) is horizontally and fixedly provided with a lifting plate (18), the central position inside the adsorption box (21) is movably clamped with a magnetic plate (40), the inside of the adsorption box (21) is provided with a disinfection mechanism (56), the inside of the adsorption box (21) is uniformly and vertically and fixedly provided with a plurality of magnetic bars (41), the magnetic bars (41) movably penetrate through the magnetic plate (40), the right end of the second electric push rod (17) is fixedly provided with a connecting rod (19), the connecting rod (19) movably penetrates through the inside of the adsorption box (21) and is fixedly arranged at the upper end of the magnetic plate (40), the right end of the adsorption box (21) is provided with a clamping groove (43), and the inside of the clamping groove (43) is fixedly provided with a sealing gasket (44), a sealing plate (20) is movably clamped and arranged at the inner position of the clamping groove (43), a cleaning groove (45) is formed in the right side position of the adsorption box (21), and a fixing mechanism (59) is arranged at the right side position of the adsorption box (21); the storage mechanism (58) comprises a one-way valve (27), a connecting pipe (26), a liquid supplementing pipe (25), a cover plate (34) and a storage box (23), a storage box (23) is fixedly arranged at the right side of the quantitative box (22) at the upper end of the reaction table (1), a liquid supplementing pipe (25) is vertically and fixedly inserted in the center of the upper end of the storage box (23), a cover plate (34) is connected at the upper end of the liquid supplementing pipe (25) in a winding way, a plurality of air inlets are arranged at the upper end of the cover plate (34), a transparent observation plate (24) is fixedly arranged at the front end of the storage box (23), a connecting pipe (26) is vertically and fixedly inserted in the inner position of the storage box (23), the left end position of the connecting pipe (26) is fixedly penetrated into the inner position of the quantitative box (22), the outer diameter of the connecting pipe (26) is larger than the inner diameter of the rubber ring (36); the fixing mechanism (59) comprises a screw (46), a movable ring (49), a limiting block (48) and a limiting plate (50), the screw (46) is horizontally and fixedly arranged at the right side end of the adsorption box (21), the limiting plate (50) is movably sleeved at the outer side end of the screw (46), the limiting block (48) is fixedly arranged at the right side end of the screw (46), and the movable ring (49) is arranged at the outer side end of the screw (46) in a winding connection mode; the sterilizing mechanism (56) comprises a lampshade (29), an ultraviolet lamp (28), a protruding block (30), a trigger switch (31) and an installing mechanism (57), the lampshade (29) is arranged at the inner position of the adsorption box (21), the ultraviolet lamp (28) is fixedly installed at the inner position of the lampshade (29), the protruding block (30) is fixedly arranged at the two side end positions in the adsorption box (21), the trigger switch (31) is fixedly installed at the upper end position of the protruding block (30), and the installing mechanism (57) is arranged at the two side end positions of the lampshade (29); the mounting mechanism (57) comprises a movable cylinder (51), a third contact (53), an insertion block (52), a spring (55) and a rectangular block (54), the insertion block (52) is fixedly mounted at the inner position of the adsorption box (21) on two sides of the ultraviolet lamp (28), the movable cylinder (51) is movably sleeved at the end positions of two sides of the lampshade (29), the rectangular block (54) is fixedly arranged at the end position of the outer side of the movable cylinder (51), the third contact (53) is fixedly mounted at the end position of the outer side of the rectangular block (54), the third contact (53) is matched with the insertion block (52), and the spring (55) is fixedly connected between the inner side of the rectangular block (54) and the outer end of the lampshade (29); the gear (6) is coaxial with the conveyor belt (2); the lifting plate (18) is smaller than the hole groove (5); the first contact (13) and the second contact (42) are electrically connected with the first electric push rod (14), and an open circuit is formed between the first contact (13) and the second contact (42).
2. The method of using a continuous nucleic acid detecting reactor according to claim 1, wherein: the method specifically comprises the following steps:
the first step is as follows: placing the test tube box (3) at the inner position of the placing groove (4), then starting a motor (47), wherein the motor (47) drives a rotating shaft (7) to rotate, so that a half gear (8) rotates, when the half gear (8) rotates to a position meshed with a gear (6), a conveyor belt (2) is driven to move for a certain distance until the half gear (8) is separated from the gear (6), at the moment, the conveyor belt (2) is in a static state, then when a conductive block (12) rotates to simultaneously contact a first contact (13) and a second contact (42), the first contact (13) is communicated with the second contact (42), and a first electric push rod (14) is driven to move downwards, and when the conductive block (12) moves to a position which is not simultaneously contacted with the first contact (13) and the second contact (42), the first electric push rod (14) is in a static state;
the second step is that: the first electric push rod (14) drives the extrusion plate (33) to move downwards when moving downwards, so that lysate in the quantitative box (22) is extruded, the lysate breaks through the rubber valve (39), then the lysate is injected into a test tube in the test tube box (3) through the injection tube (38), the pressure of the lower end of the extrusion plate (33) is reduced after injection, the rubber valve (39) is reset to seal the hollow tube (37), the communication time of the first contact (13) and the second contact (42) is the same every time, the injection amount is the same every time, quantitative injection is convenient, manual injection work is not needed, and the injection is rapid and convenient;
the third step: when the conveyor belt (2) stops, the second electric push rod (17) moves upwards to drive the lifting plate (18) to move upwards, so that the lifting plate (18) penetrates through the center position of the hole groove (5), then the lifting plate moves into the adsorption box (21), the connecting rod (19) drives the magnetic plate (40) to move upwards until the magnetic rod (41) penetrates through the center position of a test tube in the test tube box (3), then the magnetic bead is adsorbed at the outer side position of the magnetic rod (41), the second electric push rod (17) resets, the magnetic plate (40) is driven by the connecting rod (19) to move downwards, the magnetic bead at the outer side of the magnetic rod (41) is scraped, the magnetic bead is adsorbed at the lower end position of the magnetic plate (40), the concentrated collection of the magnetic bead is facilitated, the whole equipment does not need to control a control system such as a control box and the like to perform control work, and the stability is high, and the maintenance work is facilitated;
the fourth step: when the connecting rod (19) moves downwards, the magnetic plate (40) is driven to move downwards, the magnetic plate (40) extrudes the trigger switch (31), so that the ultraviolet lamp (28) is started, the magnetic bar (41) is irradiated, sterilized and disinfected, and the magnetic bar (41) is kept in a clean state when being reused;
the fifth step: the user makes activity ring (49) remove to the left side along screw rod (46) through rotating activity ring (49), extrudees limiting plate (50) and removes to the left side to extrusion fixed seal plate (20), when needing to handle the magnetic bead, user counter-rotation activity ring (49), then can dismantle sealing plate (20) to the right side, later can collect the work to the magnetic bead through clearance groove (45).
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| CN113020854B (en) * | 2021-04-28 | 2023-05-02 | 浙江德清精艺汽配有限公司 | Mechanical equipment for welding connecting rod |
| CN113355222A (en) * | 2021-05-11 | 2021-09-07 | 马永丽 | Novel gene detector and detection method |
| CN119410480A (en) * | 2025-01-03 | 2025-02-11 | 四川工商职业技术学院 | Microorganism genetic material nucleotide extraction device and application method thereof |
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