CN112370404B - Moisturizing and whitening composition, and preparation method and application thereof - Google Patents

Abstract

The invention discloses a moisturizing and whitening composition, a preparation method and application thereof, and belongs to the technical field of cosmetics. The moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 15-25 parts of vitis amurensis extract, 6-10 parts of medlar extract, 5-8 parts of neroli essential oil, 6-10 parts of liquorice extract, 2-6 parts of ginseng extract, 8-12 parts of tremella extract and 8-15 parts of aloe extract. The invention also discloses a preparation method and application of the moisturizing and whitening composition. The moisturizing and whitening composition can effectively inhibit tyrosinase activity and remove hydroxyl radicals; secondly, the product has good effects of oxidation resistance, allergy resistance, inflammation diminishing and itching relieving, and can obviously reduce anaphylactic reaction; thirdly, the skin-tendering, whitening, wrinkle-removing and freckle-removing functions are achieved; fourthly, the medicine has the effects of dredging skin capillaries and promoting blood circulation.

Description

A kind of moisturizing and whitening composition, its preparation method and application

technical field

The invention relates to a moisturizing and whitening composition, a preparation method and application thereof, and belongs to the technical field of cosmetics.

Background technique

The skin acts as a barrier in the human body, protecting the body from external factors and preventing excess water loss from the body through the skin. With age, the skin becomes dry, wrinkled, and sagging, giving it the appearance of aging. The gradual reduction of skin water content is one of the pathological mechanisms of the appearance of aging. When the water content of the stratum corneum is less than 10%, the skin becomes dry, desquamated, and cracks appear in the imbricate stratum corneum. The skin is easily eroded by the physical factors of the external environment, and the water and electrolytes in the skin are also easily evaporated or lost. Skin moisturizing is the most important basic means to ensure the barrier function of the stratum corneum and skin care and beauty.

Sensitive skin is a common troubled skin condition, and the causes are complex. It can be a comprehensive phenomenon caused by the body's endogenous factors and exogenous factors alone or together. Although the exact definition of sensitive skin has not yet been agreed upon, there has been a growing consensus in the description of the clinical symptoms and signs of their skin, namely discomfort, which in most patients is manifested almost exclusively on the face, including subjective sensory symptoms such as burning, tingling, itching , tingling, these symptoms may or may not be accompanied by erythema. Although sensitive skin is not a skin disease, the skin discomfort it causes directly affects people's quality of life. Whenever the season changes or other allergenic factors cause skin allergies, many people choose soothing sprays to relieve the symptoms of skin allergies. Most of the soothing sprays currently on the market use the water in the spray to directly act on the surface of the skin to temporarily replenish the skin with moisture, which can relieve the allergic symptoms of the surface in a short-term, but does not fundamentally change the sensitive skin. Once again stimulated by the external environment, sensitive skin will still be allergic, the face is red, swollen and itchy, with red blood or red bumps, which does not achieve the effect of anti-sensitivity and soothing skin.

Nowadays, many beauty and skin care products use chemically synthesized substances (such as chemical preservatives, preservatives and antioxidants) as the main raw materials, and most of the sprays contain chemical ingredients such as alcohol, fragrance and mineral oil. Long-term use will irritate the skin. Causes skin pigmentation and triggers allergic contact dermatitis. Some may even add some heavy metal salts in order to allow women of the oriental yellow race to achieve rapid whitening and freckle removal. These chemical additives will more or less have certain toxic and side effects on human skin, and long-term use is easy for the human body. cause health hazards.

Therefore, the development of a pure natural moisturizing and moisturizing spray with the characteristics of pure nature, no side effects, and no additives is an urgent problem for those skilled in the art to solve.

SUMMARY OF THE INVENTION

One of the objects of the present invention is to provide a moisturizing and whitening composition. The moisturizing and whitening composition of the present invention can effectively inhibit the activity of tyrosinase and scavenge hydroxyl radicals; secondly, it has good antioxidative, antiallergic, anti-inflammatory and antipruritic effects, and can obviously reduce allergic reactions; It has the functions of skin rejuvenation, whitening, wrinkle removal and freckle removal; fourth, it has the effect of dredging skin capillaries and promoting blood circulation.

The solution of the present invention to solve the above technical problems is as follows: a moisturizing and whitening composition is prepared from the following raw materials in parts by weight: 15-25 parts of sea grape extract, 6-10 parts of wolfberry extract, 5-8 parts of neroli essential oil parts, 6-10 parts of licorice extract, 2-6 parts of ginseng extract, 8-12 parts of white fungus extract and 8-15 parts of aloe vera extract.

The principle of the moisturizing and whitening composition of the present invention is:

1. Sea grape extract: Sea grape is a kind of nutritious edible green algae, 80% of which is composed of seaweed polysaccharide, collagen and dietary fiber, the amino acid composition is balanced and the variety is complete, the fat content is low and mostly unsaturated fatty acids , Rich in minerals and vitamins, it has the functions of anti-oxidation, anti-cancer, anti-tumor, blood purification, anti-inflammatory and sterilization, anti-ultraviolet and anti-aging. A total of 14 fatty acids were detected in the fatty acid composition of sea grapes. Unsaturated fatty acids accounted for 70.87% of the total fatty acids, and linoleic acid accounted for 13.14%. They are essential fatty acids for the human body. Infectious disease and other symptoms. The active ingredients in sea grape extract have antioxidant, anti-aging and anti-allergic properties.

The above sea grape extract can be purchased commercially, for example, it can be purchased from Xi'an Guohao Biotechnology Co., Ltd.

2. Lycium barbarum extract, taken from the medicinal parts of Lycium barbarum, such as Lycium barbarum. Lycium barbarum is rich in moisturizing factor, which is a new type of moisturizing agent with fast absorption and high activity. In the application of personal care products, it can quickly improve the moisture retention of skin and hair, stimulate cell vitality, and keep skin moist and smooth. , Prevents dry and dull skin.

The above-mentioned Lycium barbarum extract can be purchased commercially, for example, it can be purchased from Xi'an Zelong Biotechnology Co., Ltd.

3. Neroli essential oil: It is extracted from the white petals of bitter orange and has functions such as sterilization, cell vitality enhancement, deodorization, skin softening, whitening, moisturizing and blemish reduction. It is suitable for dry, sensitive and mature skin, especially for spiral static scars and stretch marks. At the same time, it also has the effect of calming the parasympathetic nerves, can treat insomnia, improve neuralgia, headache and dizziness.

The above-mentioned neroli essential oil can be purchased commercially, such as from Shanghai Zixin Biotechnology Co., Ltd.

4. Licorice Extract: It is extracted from licorice. The main active components are triterpenoid saponins and flavonoid glycosides, of which the content of triterpenoid saponins is 10-30%, and the content of flavonoid glycosides is 25%. -80%. Flavonoid glycosides have the functions of inhibiting tyrosinase activity, scavenging oxygen free radicals, anti-inflammatory, and anti-allergic, thereby removing yellowness and whitening.

The above-mentioned licorice extract can be purchased commercially, for example, it can be purchased from Xi'an Ruierli Bioengineering Co., Ltd.

5. Ginseng root extract: extracted from ginseng, it not only has the effect of nourishing and anti-aging, but also has the effect of soothing and whitening the skin. For the skin, ginseng contains ginsenosides, which can promote blood circulation, prevent skin aging and wrinkles, increase the nutritional supply of the skin, exert the effects of nourishing qi, nourishing the face and promoting blood circulation, and at the same time, it can also improve immune function and prevent the skin from being exposed to the outside world. Harmful stimuli of the environment. It can accelerate human metabolism, deeply moisturize the skin, prevent dryness and aging, and effectively treat itching and peeling, making facial skin delicate and smooth. Make the skin pure and tender.

The above-mentioned ginseng root extract can be purchased commercially, for example, it can be purchased from Shaanxi Huinengda Biotechnology Co., Ltd.

6. Tremella extract: It is extracted from Tremella. Tremella contains Tremella polysaccharide. Tremella polysaccharide can absorb a lot of water. It has the same high moisturizing property no matter in low humidity or high humidity conditions. Tremella is used in skin care products and has the function of repairing the epidermis and increasing the water content of the epidermis. Improve the water content of the stratum corneum, and give the skin a soft feeling, improve the skin conductivity integral value, improve the skin's water retention capacity, and reduce the water release constant; Tremella polysaccharide does not feel sticky, does not feel tight after drying, and gives the skin Moisturizing, smooth feeling, and can also fight against some free radicals, especially suitable for skin care products.

The above Tremella extract can be purchased commercially, such as from Xi'an Youshuo Biotechnology Co., Ltd.

7. Aloe vera extract: It is extracted from aloe vera. Aloe vera is the concentrated and dried sap of the leaves of the Liliaceae plant Aloe vera, Cape Aloe vera or other related plants. Modern research has proved that aloe vera has anti-cancer, anti-inflammatory, anti-viral, antibacterial, insecticidal, antipyretic and liver-protecting, and immune-enhancing effects. A medicinal plant with economic value. Anthraquinones and aloe polysaccharides are the main active ingredients in aloe vera, which have anti-inflammatory, anti-virus, diarrhea, anti-cancer, anti-aging, skin care and beauty effects.

The above-mentioned aloe vera extract can be purchased commercially, for example, it can be purchased from Shanxi Lake Biotechnology Co., Ltd.

In the prior art and in the research process of the inventors, it has been found that various traditional Chinese medicine extracts have the ability to scavenge superoxide anion free radicals, such as wolfberry, iris, liuhua, and Codonopsis. Then, when these traditional Chinese medicine extracts are used alone, the effect of scavenging superoxide anion free radicals is not obvious, and it is difficult to meet the needs of anti-aging. Therefore, in the prior art, it is also attempted to compound various traditional Chinese medicine extracts to improve the effect of scavenging superoxide anion free radicals. However, the composition of traditional Chinese medicine extracts is complex. The effect of the base is not enhanced, but decreased; or the effect is not enhanced. After screening a large number of compound formulas, the present invention surprisingly and unexpectedly found that even when the dosage is the same as that of a single component, when the sea grape extract, the wolfberry extract, the neroli essential oil, the licorice extract, the ginseng After the extract, tremella extract and aloe vera extract are compounded, a significantly enhanced effect of scavenging superoxide anion free radicals can be obtained, and the seven components have a synergistic effect. The traditional Chinese medicine extract of the present invention is actually applied to various types of cosmetics such as essence, and can also have a significant anti-aging effect. In addition, many traditional Chinese medicine extracts in the prior art are irritating, but in the present invention, it has been confirmed by experiments that sea grape extract, wolfberry extract, neroli essential oil, licorice extract, ginseng extract, tremella extract and aloe vera The composition of the extract is non-irritating.

The storage method of the moisturizing and whitening composition of the present invention: in an environment with a temperature of ≤25° C., and stored in the dark.

The beneficial effects of the moisturizing and whitening composition of the present invention are:

1. The moisturizing and whitening composition of the present invention can effectively inhibit tyrosinase activity and remove hydroxyl radicals; second, it has good antioxidative, antiallergic, anti-inflammatory and antipruritic effects, and can significantly reduce allergic reactions; Third, it has the functions of skin rejuvenation, whitening, wrinkle removal and freckle removal; fourth, it has the effect of dredging skin capillaries and promoting blood circulation.

2. The moisturizing and whitening composition of the present invention can be used to prepare various types of cosmetics, can emit natural fragrance, has no toxic and side effects, and is safe and reliable.

On the basis of the above technical solutions, the present invention can also be improved as follows.

Further, it is made from the following raw materials by weight: 15 parts of sea grape extract, 10 parts of wolfberry extract, 5 parts of neroli essential oil, 10 parts of licorice extract, 2 parts of ginseng extract, 12 parts of white fungus extract and aloe vera Extract 8 servings.

The further beneficial effects of using the above are: the above are the best parameters, and the obtained moisturizing and whitening composition has the best effect.

Further, it is made from the following raw materials by weight: 20 parts of sea grape extract, 8 parts of wolfberry extract, 6.5 parts of neroli essential oil, 8 parts of licorice extract, 4 parts of ginseng extract, 10 parts of tremella extract and aloe vera Extract 10.5 parts.

The further beneficial effects of using the above are: the above are the best parameters, and the obtained moisturizing and whitening composition has the best effect.

Further, it is made from the following raw materials by weight: 25 parts of sea grape extract, 6 parts of wolfberry extract, 8 parts of neroli essential oil, 6 parts of licorice extract, 6 parts of ginseng extract, 8 parts of tremella extract and aloe vera 15 extracts.

The further beneficial effects of using the above are: the above are the best parameters, and the obtained moisturizing and whitening composition has the best effect.

The second object of the present invention is to provide a preparation method of the above moisturizing and whitening composition. The moisturizing and whitening composition of the invention has the advantages of simple preparation method, easy operation and broad market prospect, and is suitable for large-scale popularization and application.

The solution of the present invention to solve the above-mentioned technical problems is as follows: a preparation method of a moisturizing and whitening composition, comprising the following steps:

Take the following raw materials by weight: 15-25 parts of sea grape extract, 6-10 parts of wolfberry extract, 5-8 parts of neroli essential oil, 6-10 parts of licorice extract, 2-6 parts of ginseng extract, white fungus 8-12 parts of the extract and 8-15 parts of the aloe vera extract are stirred and mixed evenly at room temperature to obtain the moisturizing and whitening composition.

On the basis of the above technical solutions, the present invention can also be improved as follows.

Further, the rotational speed of the stirring is 600r/min-1000r/min, and the time is 60min-100min.

The further beneficial effect of adopting the above is: using the above parameters, the mixing is more uniform.

The third object of the present invention is to provide the application of the above moisturizing and whitening composition. The moisturizing and whitening composition of the present invention can be used to prepare various types of cosmetics, consumers can make flexible choices according to their needs, and have broad market prospects.

The solution of the present invention to solve the above technical problems is as follows: the application of the above moisturizing and whitening composition in the preparation of cosmetics.

The beneficial effects of the application of the moisturizing and whitening composition of the present invention in the preparation of cosmetics are:

The moisturizing and whitening composition of the present invention can be used to prepare various types of cosmetics, consumers can make flexible choices according to their needs, and have broad market prospects.

On the basis of the above technical solutions, the present invention can also be improved as follows.

Further, the cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.

Further, the essence is made from the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:(0.5-5).

The further beneficial effects of adopting the above are: adopting the above ratio, the essence can be obtained.

Further, the preparation method of the essence is: by mass ratio of 1:(0.5-5), respectively weighing the above-mentioned moisturizing and whitening composition and mineral water, after mixing, at a temperature of 30 ℃-90 ℃, using a rotating speed At 600r/min-1000r/min, stirring for 60min-120min, and cooling to room temperature naturally, the essence is obtained.

The further beneficial effects of adopting the above are: adopting the above method, the essence can be prepared.

Further, the skin cream is made from the above-mentioned moisturizing and whitening composition and glycerin in a mass ratio of 1:(0.5-10).

The further beneficial effects of adopting the above are: the skin cream can be obtained by adopting the above ratio.

Further, the toner is made from the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:(11-500).

The further beneficial effects of using the above are: using the above toner, a skin cream can be obtained.

Further, the facial mask is made by coating the above-mentioned moisturizing and whitening composition on facial mask paper or facial mask cloth.

The further beneficial effect of adopting the above is: a facial mask can be obtained by adopting the above toner.

Detailed ways

The principles and features of the present invention will be described below with reference to specific embodiments. The examples are only used to explain the present invention, but not to limit the scope of the present invention.

Example 1

The moisturizing and whitening composition of this embodiment is made from the following raw materials in parts by weight: 15 parts of sea grape extract, 10 parts of wolfberry extract, 5 parts of neroli essential oil, 10 parts of licorice extract, 2 parts of ginseng extract, Tremella extract 12 parts and aloe vera extract 8 parts.

The preparation method of above-mentioned moisturizing and whitening composition, comprises the steps:

Take the following raw materials by weight: 15 parts of sea grape extract, 10 parts of wolfberry extract, 5 parts of neroli essential oil, 10 parts of licorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe vera extract , at room temperature, using a rotating speed of 600 r/min, stirring for 100 min, and after mixing evenly, the moisturizing and whitening composition is obtained.

Application of the above moisturizing and whitening composition in the preparation of cosmetics.

The cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.

The essence is composed of the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:0.5.

The preparation method of the essence is as follows: according to the mass ratio of 1:0.5, the above-mentioned moisturizing and whitening composition and mineral water are respectively weighed, and after mixing, the temperature is 30° C., the rotating speed is 600 r/min, the stirring is 120 min, and the natural cooling is carried out. After reaching room temperature, the essence is obtained.

The skin cream is made from the above-mentioned moisturizing and whitening composition and glycerin in a mass ratio of 1:0.5.

The toner is made from the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:11.

The facial mask is made by coating the above-mentioned moisturizing and whitening composition on facial mask paper or facial mask cloth.

Example 2

The moisturizing and whitening composition of this embodiment is made from the following raw materials in parts by weight: 20 parts of sea grape extract, 8 parts of wolfberry extract, 6.5 parts of neroli essential oil, 8 parts of licorice extract, 4 parts of ginseng extract, Tremella extract 10 parts and aloe vera extract 10.5 parts.

The preparation method of above-mentioned moisturizing and whitening composition, comprises the steps:

Take the following raw materials by weight: 15 parts of sea grape extract, 10 parts of wolfberry extract, 5 parts of neroli essential oil, 10 parts of licorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe vera extract , at room temperature, using a rotating speed of 800 r/min, stirring for 80 min, and after mixing evenly, the moisturizing and whitening composition is obtained.

Application of the above moisturizing and whitening composition in the preparation of cosmetics.

The cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.

The essence is composed of the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:2.

The preparation method of the essence is as follows: the above-mentioned moisturizing and whitening composition and mineral water are respectively weighed in a mass ratio of 1:2, and after mixing, at a temperature of 60° C., a rotating speed of 800 r/min, stirring for 90 min, and natural cooling. After reaching room temperature, the essence is obtained.

The skin cream is made from the above-mentioned moisturizing and whitening composition and glycerin in a mass ratio of 1:5.

The toner is made from the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:250.

The facial mask is made by coating the above-mentioned moisturizing and whitening composition on facial mask paper or facial mask cloth.

Example 3

The moisturizing and whitening composition of this embodiment is made from the following raw materials in parts by weight: 25 parts of sea grape extract, 6 parts of wolfberry extract, 8 parts of neroli essential oil, 6 parts of licorice extract, 6 parts of ginseng extract, Tremella extract 8 parts and aloe vera extract 15 parts.

The preparation method of above-mentioned moisturizing and whitening composition, comprises the steps:

Take the following raw materials by weight: 15 parts of sea grape extract, 10 parts of wolfberry extract, 5 parts of neroli essential oil, 10 parts of licorice extract, 2 parts of ginseng extract, 12 parts of tremella extract and 8 parts of aloe vera extract , at room temperature, using a rotating speed of 1000 r/min, stirring for 60 min, and after mixing evenly, the moisturizing and whitening composition is obtained.

Application of the above moisturizing and whitening composition in the preparation of cosmetics.

The cosmetic is any one of essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask.

The essence is composed of the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:5.

The preparation method of the essence is as follows: the above-mentioned moisturizing and whitening composition and mineral water are respectively weighed in a mass ratio of 1:5, and after mixing, at a temperature of 90° C., a rotating speed of 1000 r/min, stirring for 60 minutes, and natural cooling. After reaching room temperature, the essence is obtained.

The skin cream is made from the above-mentioned moisturizing and whitening composition and glycerin in a mass ratio of 1:10.

The toner is made from the above-mentioned moisturizing and whitening composition and mineral water in a mass ratio of 1:500.

The facial mask is made by coating the above-mentioned moisturizing and whitening composition on facial mask paper or facial mask cloth.

Comparative Example 1

The difference from Example 2 is that no sea grape extract is added to the raw materials of this comparative example, and the rest are the same. Specifically, the composition of this comparative example is made from the following raw materials by weight: 8 parts of wolfberry extract, 6.5 parts of neroli essential oil, 8 parts of licorice extract, 4 parts of ginseng extract, 30 parts of white fungus extract and Aloe Vera Extract 10.5 parts.

The preparation method of the above composition is the same as in Example 2.

The essence of this comparative example is composed of the above composition and mineral water in a mass ratio of 1:2.

The preparation method of the essence of this comparative example is described in Example 2.

Comparative Example 2

The difference from Example 2 is that the Tremella fungus extract is not added to the raw materials of this comparative example, and the rest are the same. Specifically, the composition of this comparative example is made from the following raw materials by weight: 30 parts of sea grape extract, 8 parts of wolfberry extract, 6.5 parts of neroli essential oil, 8 parts of licorice extract, 4 parts of ginseng extract parts and aloe vera extract 10.5 parts.

The preparation method of the above composition is the same as in Example 2.

The essence of this comparative example is composed of the above composition and mineral water in a mass ratio of 1:2.

The preparation method of the essence of this comparative example is the same as that in Example 2.

Comparative Example 3

The difference from Example 2 is that no sea grape extract and white fungus extract are added to the raw materials of this comparative example, and the rest are the same. Specifically, the composition of this comparative example is made from the following raw materials by weight: 14 parts of wolfberry extract, 12.5 parts of neroli essential oil, 14 parts of licorice extract, 10 parts of ginseng extract and 16.5 parts of aloe vera extract .

The preparation method of the above composition is the same as in Example 2.

The essence of this comparative example is composed of the above composition and mineral water in a mass ratio of 1:2.

The preparation method of the essence of this comparative example is the same as that in Example 2.

Experimental Example 1: Tyrosinase Inhibitory Activity Test

The experimental method can be found in reference ^[1] . Take 10ml of each of the essences prepared in Examples 1-3 and Comparative Examples 1-3. The samples were dissolved in phosphate buffer (pH=6.8), and each sample was prepared to be tested at a concentration of 1 mg/ml.

Take 2ml of each test group (the blank group was replaced by phosphate buffer), add tyrosinase (100U), heat in a constant temperature water tank at 37°C for 10min, then add 1ml of 0.003mol/L dopa reaction solution, stir well, After reacting at 37°C (constant temperature water bath) for 2 min, it was taken out immediately, and the absorbance at 475 nm was measured.

Inhibition rate={[(A-B)-(C-D)]/(A-B)}×100%.

A is the absorbance measured at 475nm of the mixture without sample and tyrosinase; B is the absorbance measured at 475nm of the mixture without sample and tyrosinase; C is the sample and tyrosinase added Absorbance measured at 475 nm for the mixture of aminidase; D is the absorbance measured at 475 nm for the mixture with sample but not tyrosinase added. The results are shown in Table 1.

Table 1 Inhibition rate of tyrosinase

group Inhibition rate(%) Example 1 76.8 Example 2 79.6 Example 3 77.2 Comparative Example 1 40 Comparative Example 2 48 Comparative Example 3 37

It can be seen from Table 1 that the inhibition rate of the essence prepared in Example 1-3 to tyrosinase is 76.8%-79.6%, and the inhibition rate of the essence prepared by Comparative Example 1-3 to tyrosinase is 40%- 48%, which shows that the whitening effect of the essence prepared in Example 1-3 is significantly higher than that of the essence prepared in Comparative Example 1-3. Therefore, the moisturizing and whitening composition of the present invention can effectively inhibit the activity of tyrosinase, scavenge hydroxyl radicals, and has a good antioxidant effect.

Experimental Example 2: Product Performance Test

1 Subjects and methods

1.1 Subjects

1.1.1 Inclusion criteria of subjects: (1) Normal male and female subjects aged 18-60 years old, women are preferred; (2) No serious systemic disease, no immune deficiency or autoimmune disease; (3) No Active allergic diseases; (4) No history of allergy to skin care cosmetics; (5) No systemic use of hormone drugs and immunosuppressants in the past month; (6) Those who have not participated in other clinical trials; (7) ) Volunteers who participated and were able to complete the prescribed contents according to the test requirements.

1.1.2 Exclusion conditions: (1) Women who are pregnant or breastfeeding; (2) Those who mainly work outdoors and cannot avoid sun exposure; (3) Those who increase sun exposure due to various reasons (such as travel, vacation) during the test period ; (4) Those who applied hormones and immune preparations systemically during the trial period; (5) Those who applied anti-inflammatory drugs on the face during the trial period; (6) Those who did not use the test substance as required or had incomplete data.

1.1.3 Number of subjects: 50 subjects, of which 35 subjects were in compliance with the guidelines for the evaluation of moisturizing effects of cosmetics and the guidelines for evaluation methods of efficacy of whitening cosmetics.

1.2 Methods

1.2.1 Preparation of test samples: In order to avoid microbial contamination of the samples during the preparation process, the scissors and packaging bags that are in direct contact with the essence should be sterilized before use, and the personnel preparing the samples should wear gloves to operate.

1.2.2 Facial test sites: the forehead, chin, left cheek and right cheek of the face are used as test areas.

1.2.3 Face test method: Whitening products (cosmetics, external medicines or oral health products) cannot be used 2-3 days before the face test. Before the test, the subjects need to uniformly clean the facial skin and wipe it with dry facial tissue. The subjects were asked to sit still for at least 30 minutes in a room that met the standard, with the facial skin exposed, not rubbing, and kept relaxed.

1.2.4 Test duration: Periodic efficacy evaluation, 3 times a week, continuous testing for 12 weeks.

1.2.5 Evaluation: The trial effect of the essence of Example 2 was evaluated by means of a questionnaire, wherein the moisturizing property was evaluated by a skin moisture tester, and the appearance and whitening and brightening effects were evaluated by the subjects' sensory evaluation; uniformly apply 2ml of the essence .

Table 2 Sensory evaluation of facial mask

1.3 Experimental results

A sensory test was conducted on 50 qualified subjects, and 2 people withdrew due to different reasons. Finally, 48 complete test results were obtained. The test results were divided into three grades: general, satisfactory and very satisfied. The number of people is counted, and the corresponding test results are obtained. The whole test results are shown in the following table:

Table 3 The sensory evaluation result table of the essence obtained in Example 2

2. Mask moisturizing test measured by skin moisture analyzer

2.1 Experimental instrument

Digital skin tester.

2.2 Principle

The moisture content of the skin is determined by both internal and external factors. The ability of the stratum corneum to retain moisture varies greatly. The moisture content can vary from 10% to 60%. The most important thing is the breathing process of the skin sweating and the skin. The composition of the water mixture. External factors, including ambient temperature, humidity, medicines and cosmetics, can determine and change the moisture content of the skin. The moisture content of the skin will affect the formation of a mixed film of water and oil on the skin surface, and this protective film is very important for skin aging. Therefore, it is useful to quantitatively test the moisture content of the skin and the amount of change relative to a certain treatment period.

2.3 Experimental method

Strong efficacy products (cosmetics, topical medicines or oral health supplements) cannot be used 2-3 days before the facial test. Before the test, the subjects need to uniformly clean the facial skin and wipe it with dry facial tissue. The subjects were asked to sit still for at least 30 minutes in a room that met the standard, with the facial skin exposed, not rubbing, and kept relaxed. The moisture content of the forehead and cheeks of the skin was measured with a skin moisture meter, and the moisture content of the skin before the test was read and recorded.

Apply 2ml of essence, remove after 15 minutes, tap until absorbed. The essences are the essences obtained in Example 2 and Comparative Examples 1-3, respectively. Do not use any facial products within 12 hours, test after 10min, 30min, 60min and 12h respectively, and record the skin moisture after the test.

The average increase value method (increase value = skin moisture after test - skin moisture before test) was used to obtain the moisture increase value of the facial skin after using the product.

2.4 Experimental results

As shown in Table 4-Table 7.

The essence that table 4 embodiment 2 obtains

Table 5 Essence obtained in Comparative Example 1

Table 6 Essence obtained in Comparative Example 2

Table 7 Essence obtained in Comparative Example 3

It can be seen from the comparison between Table 4 and Table 5 that in the essence of Comparative Example 1, since no sea grape extract was added, the effects of anti-oxidation, anti-aging and allergy inhibition were weak.

It can be seen from the comparison between Table 4 and Table 6 that in the essence of Comparative Example 2, since no Tremella extract was added, the moisturizing properties and antioxidant effects were weak.

From the comparison of Table 4 and Table 7, it can be seen that in the essence of Comparative Example 3, since no sea grape extract and white fungus extract are added, the effects of anti-oxidation, anti-aging, allergy inhibition and moisturizing are weak.

To sum up, the moisturizing and whitening composition of the present invention can effectively inhibit the activity of tyrosinase and scavenge hydroxyl radicals; ; Third, it has the functions of skin rejuvenation, whitening, wrinkle removal and freckle removal; Fourth, it has the effect of dredging skin capillaries and promoting blood circulation.

references:

1. Wei Zhencheng, Sun Ling, Zhang Yan, et al. Study on the inhibitory effect of soybean isoflavones on tyrosinase [J]. Guangzhou Food Industry Science and Technology, 2004, 20(3): 10-11.

The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection of the present invention. within the range.

Claims (7)

1. a moisturizing and whitening composition, is characterized in that, is made of the raw material of following parts by weight: 20 parts of sea grape extracts, 8 parts of wolfberry extracts, 6.5 parts of neroli essential oil, 8 parts of licorice extracts, ginseng extracts 4 parts of Tremella extract, 10 parts of Tremella extract and 10.5 parts of aloe vera extract. 2. a preparation method of moisturizing and whitening composition, is characterized in that, comprises the steps: Take the following raw materials by weight: 20 parts of sea grape extract, 8 parts of wolfberry extract, 6.5 parts of neroli essential oil, 8 parts of licorice extract, 4 parts of ginseng extract, 10 parts of tremella extract and 10.5 parts of aloe vera extract , at room temperature, after stirring and mixing evenly, the moisturizing and whitening composition is obtained. 3. The preparation method of moisturizing and whitening composition according to claim 2, is characterized in that, the rotating speed of described stirring is 600 r/min-1000 r/min, and the time is 60 min-100 min. 4. The application of the moisturizing and whitening composition of claim 1 in the preparation of cosmetics. 5. application according to claim 4 is characterized in that, described cosmetic is any one in essence, skin cream, lotion, gel, toner, spray water, eye patch and facial mask. 6. application according to claim 5, is characterized in that, described essence is made by the moisturizing and whitening composition described in claim 1 and mineral water in mass ratio of 1:(0.5-5); Cream is made by the moisturizing and whitening composition of claim 1 and glycerin by mass ratio of 1:(0.5-10); the toner is made by the moisturizing and whitening composition of claim 1 and mineral water by mass ratio of 1 : (11-500) is made; Described facial mask is made by coating on facial mask paper or facial mask cloth by the moisturizing and whitening composition described in claim 1. 7. application according to claim 6, is characterized in that, the preparation method of described essence is: be 1:(0.5-5) by mass ratio, take by weighing the moisturizing whitening composition described in claim 1 and Mineral water, after mixing, at a temperature of 30 ℃-90 ℃, using a rotating speed of 600 r/min-1000 r/min, stirring for 60 min-120 min, and cooling to room temperature naturally to obtain the essence.