CN112262878B - Method for rapidly cleaning large intestine of pig - Google Patents
Method for rapidly cleaning large intestine of pig Download PDFInfo
- Publication number
- CN112262878B CN112262878B CN202010986745.5A CN202010986745A CN112262878B CN 112262878 B CN112262878 B CN 112262878B CN 202010986745 A CN202010986745 A CN 202010986745A CN 112262878 B CN112262878 B CN 112262878B
- Authority
- CN
- China
- Prior art keywords
- large intestine
- cleaning composition
- glucose derivative
- pig
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
- A22C—PROCESSING MEAT, POULTRY, OR FISH
- A22C17/00—Other devices for processing meat or bones
- A22C17/16—Cleaning of intestines; Machines for removing fat or slime from intestines
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/662—Carbohydrates or derivatives
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2075—Carboxylic acids-salts thereof
- C11D3/2079—Monocarboxylic acids-salts thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/22—Carbohydrates or derivatives thereof
- C11D3/222—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/34—Organic compounds containing sulfur
- C11D3/349—Organic compounds containing sulfur additionally containing nitrogen atoms, e.g. nitro, nitroso, amino, imino, nitrilo, nitrile groups containing compounds or their derivatives or thio urea
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Emergency Medicine (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a method for quickly cleaning a large intestine of a pig, which relates to the technical field of food processing and comprises the following steps: a material preparation step of soaking the large intestine with salt and white vinegar, a primary washing step of decontaminating and degreasing, and a fine washing step of adopting a cleaning composition and an intermittent vacuum rolling and kneading process; the cleaning composition comprises a glucose derivative A. The glucose derivative A has a molecular formula of C16H18N2O6And the molecular weight is 322.31. The cleaning method provided by the invention adopts the mode that the cleaning composition is matched with a vacuum rolling and kneading process to clean the pig large intestine, so that the peculiar smell and fat oil on the surface of the pig large intestine can be effectively removed, the elasticity and chewiness of the large intestine are increased, the texture of the large intestine is improved, cholesterol can be decomposed, the cholesterol content of the large intestine and the greasy feeling during eating are reduced, the cleaned large intestine has glossy appearance, no peculiar smell or odor, no exudate and no stickiness, and a user can obtain better sensory experience.
Description
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a method for quickly cleaning a large intestine of a pig.
Background
The pig large intestine is an internal organ of a pig, is mainly used for conveying and digesting food, has strong toughness, is not as thick as a pig belly, also has a proper amount of fat and high-content cholesterol, is also called a fat intestine, is a common pig internal organ subsidiary food, and is one of foods loved by people in daily life. The large intestine of pig is cold in nature and sweet in taste, and has the functions of moistening intestine, removing lower energizer wind-heat and relieving frequent micturition.
The large intestine of the pig is delicious and can provide rich nutrition, but the large intestine of the pig can generate strong peculiar smell in a natural state or when the processing is not proper, and the large intestine of the pig has more folds and more stains, is not easy to clean and clean. And the pig large intestine contains more fat and cholesterol, is easy to become greasy due to improper processing, and also increases the risk of obesity. At present, people often use various chemical agents to clean the large intestine of the pig, and drug residues are easy to form. Secondly, the large intestine is easily polluted by microorganisms within the processing lag time, is easy to cause the propagation of the microorganisms and even cause food-borne diseases, and is a 'high-risk food' which is easy to cause food poisoning. Common contaminating microorganisms are food-borne pathogenic bacteria such as escherichia coli and staphylococcus aureus. Therefore, the present inventors have conducted new studies on the cleaning composition and cleaning conditions for porcine large intestine to provide a new cleaning method more thoroughly.
Disclosure of Invention
The invention aims to provide a method for quickly cleaning the large intestine of a pig, which can effectively remove peculiar smell and fat oil on the surface of the large intestine of the pig, increase the elasticity and chewiness of the large intestine, improve the texture of the large intestine, decompose cholesterol, reduce the cholesterol content of the large intestine and reduce the greasy feeling when the pig is eaten.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a method of rapidly cleansing the large intestine of a pig comprising:
a material preparation step of soaking the large intestine with salt and white vinegar, a primary washing step of decontaminating and degreasing, and a fine washing step of adopting a cleaning composition and an intermittent vacuum rolling and kneading process;
the cleaning composition comprises a glucose derivative A shown in the following structural formula:
according to the technical scheme, the pig large intestine is cleaned by matching the cleaning composition with a vacuum rolling and kneading process, so that oil and stain can be removed easily, stains and fat oil on the surface of the pig large intestine can be removed effectively, the elasticity and chewiness of the large intestine are improved, the texture of the large intestine is improved, the cholesterol content of the large intestine and the greasy feeling during eating are reduced, the cleaned large intestine has glossy appearance, no peculiar smell or odor, no exudate and no stickiness, and a better sensory experience is obtained for eaters; the residue is harmless to human body.
For the invention, in the material preparation step, the addition amounts of the salt and the white vinegar are respectively 0.3-0.5 time and 0.5-1 time of the weight of the large intestine; the soaking time is 30-45 min. In the specific implementation method, the adding amount of the salt and the white vinegar is 0.3-0.5 time and 0.5-1 time of the weight of the large intestine respectively. Preferably, the large intestine is cleaned for 2-3 times by adopting clear water after soaking.
Preferably, the specific implementation method of the initial washing step is as follows: turning over the prepared large intestine outwards, removing dirt and lymph adhered on the surface, trimming redundant grease with scissors, cleaning for 2-3 times with clear water, and then turning over the large intestine outwards.
For the invention, in the fine washing step, the vacuum degree of the vacuum tumbling process is-0.1 to-0.08 Mpa, and the tumbling time is 30 to 60 min. Preferably, after the rolling is finished, the rolled large intestine is cleaned by clean water, drained and stored in vacuum for later use.
For the invention, in the fine washing step, the operation conditions of the intermittent vacuum tumbling process are as follows: the single forward rotation time is 15-20 min, the single reverse rotation time is 15-20 min, the idle time during steering exchange is 3-5 min, and the rotating speed is 50-60 r/min. Through the process of vacuumizing and rolling, the components in the cleaning composition can enter villus holes and folds of the large intestine more deeply, and viscous liquid on the surface of the large intestine can be fully adsorbed, so that the intestine body is cleaner, and the cleaning effect can be more thorough.
The cleaning composition comprises, by weight, 25-35% of white vinegar, 20-25% of starch, 0.01-3% of acesulfame potassium, 78-10% of glucose derivative A3 and 35-50% of water. Preferably, the above-mentioned cleansing composition is added in an amount of 0.5 to 1.5 times the weight of the large intestine. The acesulfame potassium as a flavoring agent can improve the taste and smell of large intestine, reduce the stimulation on sense organs, and make people sensitive to taste and smell more easily accept and eat.
The invention also provides a large intestine cleaning composition used in the method for quickly cleaning the large intestine of the pig, wherein the cleaning composition comprises a glucose derivative A shown in the following structural formula:
the glucose derivative A has a molecular formula of C16H18N2O6And the molecular weight is 322.31. The large intestine cleaning composition is added with starch with a sticky function, a flavoring agent, a glucose derivative A and the like, so that sticky greasy dirt in the large intestine of a pig can be effectively removed, the greasy dirt and grease on the surface of the large intestine of the pig can be removed, peculiar smell and blood stain can be removed, the elasticity and the chewiness of the large intestine can be increased, the texture of the large intestine can be improved, a certain cholesterol decomposition function is shown, the cholesterol content of the large intestine is reduced, the greasy feeling of the large intestine of the pig during eating can be reduced, and the large intestine food is healthier.
For the present invention, the content of the glucose derivative A in the composition for cleaning large intestine is 3-10 wt%.
Preferably, the large intestine cleaning composition comprises, by percentage, 25-35 wt% of white vinegar, 20-25 wt% of starch, 0.01-3 wt% of acesulfame potassium, 3-10 wt% of glucose derivative A and 35-50 wt% of water. More preferably, the cleaning composition further comprises 0-0.5 wt% of aromatic syrup, and specific examples include: such as orange peel syrup, licorice syrup, and cinnamon syrup. The aromatic syrup can correct taste and odor.
The invention also provides a glucose derivative A, which has the following structural formula:
and the use of the glucose derivative A in the preparation of the large intestine cleaning composition. The glucose derivative A can effectively remove greasiness, grease and odor of pig large intestine surface, increase elasticity and chewiness of large intestine, and improve texture of large intestine, thereby reducing cholesterol content of pig large intestine and greasiness during eating, and making large intestine food have good taste and health.
For the purposes of the present invention, the glucose derivative A has the effect of reducing the cholesterol content in the large intestine. The glucose derivative A has no harm to human body and cytotoxicity, and can remarkably reduce cholesterol content in large intestine and make food healthier.
The invention adopts the cleaning mode of the cleaning composition and the vacuum rolling and kneading process, thereby having the following beneficial effects: 1) the cleaning method can effectively remove odor, stain and fat oil on the surface of the pig large intestine, increase elasticity and chewiness of the large intestine, improve the texture of the large intestine, reduce the cholesterol content of the large intestine and the greasy feeling when eating, and the residues are harmless to human body; 2) starch with a sticky effect, a flavoring agent, a glucose derivative A and the like are added into the large intestine cleaning composition provided by the invention, so that the large intestine cleaning composition has a certain cholesterol decomposition effect, can effectively remove sticky greasy stains in the large intestine of a pig, reduces the greasy feeling of the large intestine of the pig when the large intestine is eaten, enables the large intestine food to have better mouthfeel and be healthier, and residues of the large intestine cleaning composition are harmless to a human body and have no cytotoxicity; 3) the glucose derivative A can increase elasticity and chewiness of large intestine, improve texture of large intestine, and decompose cholesterol.
Drawings
FIG. 1 is a graph of the effect of different washing methods on the texture of the large intestine;
FIG. 2 is a graph of the effect of different washing methods on the cholesterol content of the large intestine;
FIG. 3 shows the effect of different cleaning methods on the pathogenic bacteria on the surface of the large intestine, A-Staphylococcus aureus and B-Escherichia coli;
FIG. 4 shows the inhibitory effect of different cleaning methods on pathogenic bacteria on the surface of the large intestine, A-Staphylococcus aureus and B-Escherichia coli.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
in a specific implementation scenario, the synthesis method of the glucose derivative a is as follows:
1) stirring and dissolving pentaacetylglucose, 4-hydroxy-6-aminoquinoline and Lewis acid in an organic solvent, reducing the pressure to 4-50 kPa, then heating to 80-120 ℃ for reaction for 3.5-5 h, and then preserving heat and aging for 1-2 h to obtain an intermediate product;
2) and (2) carrying out ester exchange reaction on the intermediate product and methanol at the temperature of 40-70 ℃ for 3-5 hours under the action of a catalyst, then adjusting the pH value of a system to be 8-10 by using a NaOH solution with the volume concentration of 20-40%, filtering while the solution is hot to remove unreacted pentaacetylglucose, then cooling to normal temperature, adding distilled water according to the volume ratio of 1:1, removing precipitates, and distilling to remove water to obtain the precipitate, namely the glucose derivative A.
Preferably, in the step 1), the molar ratio of the 4-hydroxy-6-aminoquinoline to the pentaacetylglucose is 1: 3-6, and the molar ratio of the Lewis acid to the pentaacetylglucose is 1: 0.03-0.08. More preferably, the pentaacetylglucose is beta-pentaacetylglucose,
preferably, in the step 1), the amount of the organic solvent is 3-6 times of the weight of the pentaacetylglucose; the organic solvent is chloroform, dichloromethane or dichloroethane. The Lewis acid is zinc chloride or magnesium chloride, preferably magnesium chloride.
Preferably, in the step 2), the amount of the methanol is 5-10 times of the weight of the intermediate product. The catalyst is sodium methoxide or sodium ethoxide, preferably sodium methoxide. The dosage of the catalyst is 0.03-0.07 time of the weight of the intermediate product.
As an improvement to the technical scheme of the invention, the method is further provided with the following steps: before vacuum tumbling, dioctyl sodium sulfosuccinate accounting for 0.01-0.25% of the weight of the large intestine and quinic acid accounting for 0.03-0.15% of the weight of the large intestine are added into the system. Probably, the two ingredients can keep better effective acidity in the storage process in a synergistic manner, have a certain inhibition effect on pathogenic bacteria, can provide good decontamination and deoiling performance, and endow the large intestine with long-acting antibacterial effect after washing, so that the beneficial effects of reducing the residual quantity of the pathogenic bacteria on the surface of the large intestine, obtaining better cleaning effect, achieving better inhibition effect on the residual pathogenic bacteria in the large intestine during storage and having long antibacterial duration are achieved.
The present invention and the conventional techniques in the embodiments are known to those skilled in the art and will not be described in detail herein.
It is to be understood that the foregoing description is to be considered illustrative or exemplary and not restrictive, and that changes and modifications may be made by those skilled in the art within the scope and spirit of the appended claims. In particular, the present invention covers other embodiments having any combination of features from the different embodiments described above and below, without the scope of the invention being limited to the specific examples below.
Example 1:
a method for synthesizing a glucose derivative A comprises the following steps:
1) stirring and dissolving beta-pentaacetylglucose, 4-hydroxy-6-aminoquinoline and Lewis acid magnesium chloride in an organic solvent, reducing the pressure to 10kPa, then heating to 110 ℃ for reaction for 4h, and then preserving the heat and aging for 2h to obtain an intermediate product; the molar ratio of the 4-hydroxy-6-aminoquinoline to the beta-pentaacetylglucose is 1:4, the molar ratio of the Lewis acid magnesium chloride to the beta-pentaacetylglucose is 1:0.08, and the dosage of the organic solvent chloroform is 6 times of the weight of the beta-pentaacetylglucose;
2) and (2) carrying out ester exchange reaction on the intermediate product and methanol at the temperature of 60 ℃ for 4.5 hours under the action of catalyst sodium methoxide, then adjusting the pH value of a system to be 9.0 by using NaOH solution with the volume concentration of 30%, filtering to remove unreacted pentaacetylglucose while the system is hot, then cooling to normal temperature, adding distilled water according to the volume ratio of 1:1, removing precipitates, distilling to remove water, and obtaining the precipitate, namely the glucose derivative A. The amount of the methanol is 6 times of the weight of the intermediate product; the amount of sodium methoxide used as catalyst is 0.07 times of the weight of the intermediate product.
And (3) determination: the molecular formula of the glucose derivative A is C16H18N2O6The molecular weight of the copolymer is 322.31,1HNMR(CD3OD,TMS)δPPm:8.93(1H,s),8.89(1H,s),7.81(1H,s),7.43(1H,s),7.30(1H,s),6.27(2H,s),5.88(1H,s),3.58-3.65(4H,m),3.49-3.91(4H,m),3.54-3.79(2H,d)。
example 2:
a large intestine cleaning composition is used in the method for rapidly cleaning the large intestine of the pig, and comprises 25 wt% of white vinegar, 22.5 wt% of starch, 2.5 wt% of acesulfame potassium, 5 wt% of glucose derivative A prepared in example 1 and 45 wt% of water.
The preparation method of the large intestine cleaning composition comprises the following specific steps: mixing acesulfame potassium and the glucose derivative A prepared in example 1, adding water, heating to 60 ℃, stirring for 30min, cooling, adding white vinegar, stirring for 5min, adding starch, and stirring until no granules exist.
Example 3:
a method of rapidly cleansing the large intestine of a pig comprising:
1) preparing materials: placing the pre-washed large intestine in a clean container, adding prepared salt and white vinegar, soaking for 30min, and cleaning with clear water for 2 times; the addition amount of the salt and the white vinegar is 0.3 times and 0.5 times of the weight of the large intestine respectively;
2) initial washing: turning over the large intestine, removing dirt and lymph on the surface, trimming off excessive oil with scissors, cleaning with clear water for 3 times, and turning over the large intestine;
3) fine washing: placing the primarily washed large intestine in a vacuum rolling and kneading pot with the vacuum degree of-0.08 Mpa, adding the large intestine cleaning composition prepared in the example 2, performing vacuum rolling and kneading for 30min, cleaning the rolled large intestine with clear water, draining off water, and storing in vacuum for later use. The addition amount of the cleaning composition is 0.5 time of the weight of the large intestine; the rolling and kneading are carried out in an intermittent manner, which comprises the following steps: the single forward rotation time is 15min, the single reverse rotation time is 15min, the down time during the steering change is 5min, and the rotating speed is 50 r/min.
Example 4:
a method for rapidly cleaning the large intestine of a pig, which is different from the method in the embodiment 3 in that:
in the step 3), before vacuum tumbling, dioctyl sodium sulfosuccinate accounting for 0.15% of the weight of the large intestine and quinic acid accounting for 0.1% of the weight of the large intestine are also added into the system.
Example 5:
a method for rapidly cleaning the large intestine of a pig, which is different from the method in the embodiment 3 in that:
in the step 3), the large intestine cleaning composition comprises 25 wt% of white vinegar, 22.5 wt% of starch, 2.5 wt% of acesulfame potassium and 50 wt% of water; i.e. the cleaning composition does not contain glucose derivative a.
Example 6:
a method for rapidly cleaning the large intestine of a pig, which is different from the method in the embodiment 4 in that:
in the step 3), before vacuum tumbling, dioctyl sodium sulfosuccinate accounting for 0.15% of the weight of the large intestine is added into the system, and quinic acid is not added.
Example 7:
a method for rapidly cleaning the large intestine of a pig, which is different from the method in the embodiment 4 in that:
in the step 3), before vacuum tumbling, quinic acid accounting for 0.1 percent of the weight of the large intestine is added into the system, and dioctyl sodium sulfosuccinate is not added.
Experimental example 1:
effect of different washing methods on the texture of the Large intestine
The experimental method comprises the following steps: the same weight of large intestine was taken and washed with clean water as a blank group according to the washing methods of examples 3, 4 and 5, respectively. Texture profile analysis was used to determine the texture of the large intestine before and after washing, and the measurement items included elasticity and chewiness. The measurement parameters are as follows: 1mm/s, speed in the middle of measurement: 5mm/s, speed after measurement: 5 mm/s; interval time between two presses: 5s, load type: auto (force); the probe type: TPA was P36R, data collection rate: 250 fps. Sample specification: large intestine having a thickness of 5.00cm, ambient temperature at the time of measurement: at 25 ℃. The results are shown in FIG. 1.
FIG. 1 is a graph showing the effect of different washing methods on the texture of the large intestine. The results show that the large intestine of each example shows a tendency to increase in elasticity and chewiness. Wherein the differences between example 3 and example 4 were not significant and showed significantly higher elasticity and chewiness data for example 3 than for example 5. The elasticity and chewiness of example 3 were increased to 0.86mm and 2852.47g, respectively, and the elasticity and chewiness of example 5 were 0.62mm and 1623.58g, respectively. The result shows that after the glucose derivative A is added into the cleaning composition, the peculiar smell and fat oil of the large intestine can be cleaned, the elasticity and chewiness of the large intestine can be increased, the texture of the large intestine is improved, and a user can obtain better sensory experience.
Experimental example 2:
effect of different washing methods on the Cholesterol content of the Large intestine
The experimental method comprises the following steps: the same weight of large intestine was washed according to the washing methods of examples 3, 4 and 5, and the cholesterol content in the large intestine was measured before and after washing, and measured according to the method for measuring the cholesterol content in meat and meat products of GB/T9695.24-2008, and the results are shown in FIG. 2.
FIG. 2 is a graph showing the effect of different washing methods on the cholesterol content of the large intestine. The results show that after different cleaning methods, the cholesterol content in the large intestine changes obviously, wherein the difference between the example 3 and the example 4 is not significant, the cholesterol content shows a remarkable descending trend, the example 3 reduces from 163.3mg/100g to 96.8mg/100g, the example 4 reduces from 165.2mg/100g to 97.1mg/100g, and the example 5 does not change obviously and reduces from 162.8mg/100g to 148.2mg/100g before cleaning. The result shows that after the glucose derivative A is added into the cleaning composition, peculiar smell and fat oil of the large intestine can be cleaned, the cholesterol content in the large intestine can be effectively reduced, although the specific mechanism is yet to be verified, the beneficial effect is obvious, and the large intestine food can be healthier.
Experimental example 3:
influence of different cleaning methods on cleaning effect of pathogenic bacteria on surface of large intestine
The experimental method comprises the following steps: activating glycerol-preserved Staphylococcus aureus and Escherichia coli, inoculating into broth culture medium, shake culturing at 37 deg.C for 24 hr, centrifuging 1mL to obtain cells, diluting with sterile physiological saline to 10%8cfu/mL for standby; then the inoculation liquid is fully contacted with the large intestine with the same weight before cleaning, so that the inoculation concentration of the two strains on the surface of the large intestine is not less than 106cfu/cm2. The large intestine was washed according to the washing methods of examples 3, 4, 6 and 7, respectively. After the fine wash step was completed, the large intestine was drained and placed in a sterile bag containing 50mL of sterile MRD buffer (0.1% peptone and 8.5% NaCl) and shaken 30 times to disperse the bacterial cells. Taking 1mL of each buffer solution, and respectively culturing the buffer solution with BairdPaker agar medium (staphylococcus aureus) and crystal violet neutral red bile salt agar VRBA medium (escherichia coli) at 37 ℃ for 48 DEG Ch, respectively counting the number of the residual pathogenic bacteria (cfu/cm)2) Converted into log cfu/cm2. Each group was 3 replicates. The results are shown in FIG. 3.
FIG. 3 shows the effect of different cleaning methods on pathogenic bacteria on the surface of the large intestine, A-Staphylococcus aureus and B-Escherichia coli. The results show that the residual amounts of both species decreased overall as the wash proceeded. However, the cleaning effect of example 4 is far superior to that of example 3, and also superior to that of examples 6 and 7 in terms of the final residual amount; the initial inoculation amount is 6log cfu/cm2Example 4 after washing, the residual amounts of Staphylococcus aureus and Escherichia coli were 3.8log cfu/cm, respectively2And 3.2log cfu/cm2(ii) a After the cleaning of example 3, the residual amounts of Staphylococcus aureus and Escherichia coli were 4.7log cfu/cm, respectively2And 4.3log cfu/cm2. The cleaning method of example 4 can reduce the residual quantity of pathogenic bacteria on the surface of the large intestine and obtain better cleaning and sterilizing effects while providing good decontamination and oil removal performance.
Experimental example 4:
inhibiting effect of different cleaning methods on pathogenic bacteria on surface of large intestine in storage period
The experimental method comprises the following steps: the same weight of large intestine was used and the large intestine was washed by the washing methods of examples 3, 4, 6 and 7. The inoculation concentration of Staphylococcus aureus and Escherichia coli on the surface of large intestine is 10% according to the method in example 34cfu/cm2. Placing into a sterile bag after inoculation, and storing at 4 ℃ for 24 h. Then, the cells were taken out and put into a sterile bag containing 50mL of a sterile MRD buffer (0.1% peptone and 8.5% NaCl) and shaken 30 times to disperse the bacterial cells. Thereafter, the above procedure was repeated by sampling every 1 week for 4 weeks. And counting the number of residual pathogenic bacteria (cfu/cm)2) Converted into log cfu/cm2. Each group was 3 replicates. And drawing a growth inhibition curve of the residual pathogenic bacteria under the storage condition of 4 ℃. The results are shown in FIG. 4.
FIG. 4 shows the inhibitory effect of different cleaning methods on pathogenic bacteria on the surface of the large intestine, A-Staphylococcus aureus and B-Escherichia coli. The results show the number of Staphylococcus aureus at 4 ℃ storageThe amount of the compound is increased, but the compound in example 4 has a remarkable inhibitory effect on Staphylococcus aureus compared with that in example 3, so that the increase of the amount of the compound in the storage period is low, and the amount of the compound in example 4 is 4.0log cfu/cm2Increased to 4.9log cfu/cm2The amount of example 3 was measured from 4.0log cfu/cm2Increased to 6.3log cfu/cm2. Similarly, example 4 showed a more significant inhibition of E.coli than example 3 under storage conditions of 4 ℃ so that the E.coli count of example 4 was from 4.0log cfu/cm in the first 2 weeks of storage2Increase to 4.8log cfu/cm2Then the number was reduced to 4.5log cfu/cm2Lower than the initial level, the inhibition effect on escherichia coli is very obvious; while example 3 has a relatively slow growth trend of 4.0log cfu/cm2Increased to 5.8log cfu/cm2。
In summary, the cleaning method of example 4 provides good degreasing and cleaning effects, and also provides long-lasting antibacterial effect to the large intestine after washing, and has excellent inhibitory effect on residual pathogenic bacteria in the large intestine during storage, and long antibacterial duration.
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (9)
1. A method of rapidly cleansing the large intestine of a pig comprising:
a material preparation step of soaking the large intestine with salt and white vinegar, a primary washing step of decontaminating and degreasing, and a fine washing step of adopting a cleaning composition and an intermittent vacuum rolling and kneading process;
the cleaning composition comprises a glucose derivative A shown in the following structural formula:
2. the method of claim 1, wherein the method comprises the following steps: in the material preparation step, the addition amounts of salt and white vinegar are respectively 0.3-0.5 time and 0.5-1 time of the weight of the large intestine; and soaking the large intestine for 30-45 min by using salt and white vinegar.
3. The method of claim 1, wherein the method comprises the following steps: in the fine washing step, the vacuum degree of the vacuum tumbling process is-0.1 to-0.08 Mpa, and the tumbling time is 30 to 60 min.
4. The method of claim 1, wherein the method comprises the following steps: in the fine washing step, the operation conditions of the intermittent vacuum tumbling process are as follows: the single forward rotation time is 15-20 min, the single reverse rotation time is 15-20 min, the idle time during steering exchange is 3-5 min, and the rotating speed is 50-60 r/min.
5. The method of claim 1, wherein the method comprises the following steps: the cleaning composition comprises, by weight, 25-35% of white vinegar, 20-25% of starch, 0.01-3% of acesulfame potassium, 3-10% of glucose derivative A and 35-50% of water.
6. A large intestine cleaning composition for use in the method for rapidly cleaning the large intestine of a pig according to claim 1, wherein: the cleaning composition comprises a glucose derivative A shown in the following structural formula:
the molecular formula of the glucose derivative A is C16H18N2O6And the molecular weight is 322.31.
7. The large intestine cleaning composition according to claim 6, wherein: the content of the glucose derivative A in the large intestine cleaning composition is 3-10 wt%.
9. The glucose derivative A according to claim 8, wherein: the glucose derivative A has the effect of reducing the content of cholesterol in large intestine.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010986745.5A CN112262878B (en) | 2020-09-18 | 2020-09-18 | Method for rapidly cleaning large intestine of pig |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010986745.5A CN112262878B (en) | 2020-09-18 | 2020-09-18 | Method for rapidly cleaning large intestine of pig |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112262878A CN112262878A (en) | 2021-01-26 |
CN112262878B true CN112262878B (en) | 2022-05-17 |
Family
ID=74348980
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010986745.5A Active CN112262878B (en) | 2020-09-18 | 2020-09-18 | Method for rapidly cleaning large intestine of pig |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112262878B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116671602A (en) * | 2023-06-01 | 2023-09-01 | 中国肉类食品综合研究中心 | Method for reducing peculiar smell of fat sausage and viscera of fat sausage products |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1215310A (en) * | 1996-03-06 | 1999-04-28 | 诺沃挪第克公司 | Method of killing or inhibiting microbial cells |
CN104163806A (en) * | 2013-05-20 | 2014-11-26 | 重庆圣华曦药业股份有限公司 | Preparation method of triacetylglucal |
CN109020885A (en) * | 2018-09-11 | 2018-12-18 | 中国人民解放军成都军区总医院 | Aminoquinolines and its preparation method and application |
CN109666082A (en) * | 2017-10-13 | 2019-04-23 | 上海东升新材料有限公司 | A kind of 8- aminoquinoline modified starch chelating agent and preparation method thereof |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6573375B2 (en) * | 2000-12-20 | 2003-06-03 | Union Carbide Chemicals & Plastics Technology Corporation | Liquid thickener for surfactant systems |
-
2020
- 2020-09-18 CN CN202010986745.5A patent/CN112262878B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1215310A (en) * | 1996-03-06 | 1999-04-28 | 诺沃挪第克公司 | Method of killing or inhibiting microbial cells |
CN104163806A (en) * | 2013-05-20 | 2014-11-26 | 重庆圣华曦药业股份有限公司 | Preparation method of triacetylglucal |
CN109666082A (en) * | 2017-10-13 | 2019-04-23 | 上海东升新材料有限公司 | A kind of 8- aminoquinoline modified starch chelating agent and preparation method thereof |
CN109020885A (en) * | 2018-09-11 | 2018-12-18 | 中国人民解放军成都军区总医院 | Aminoquinolines and its preparation method and application |
Also Published As
Publication number | Publication date |
---|---|
CN112262878A (en) | 2021-01-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2893953B2 (en) | Bifidobacterium growth promoter | |
CN105341137A (en) | Fresh shrimp freezing and antistaling agent as well as fresh shrimp freezing and antistaling method | |
US4115591A (en) | Process for producing koji and utilization of the koji | |
Ashenafi | The microbiology of Ethiopian foods and beverages: A review | |
JP2003516738A (en) | Tofu containing lactic acid bacteria culture solution, method for producing the same, and beverage containing lactic acid bacteria and method for producing the same | |
CN112262878B (en) | Method for rapidly cleaning large intestine of pig | |
JP2001149041A (en) | Composition based on mannooligosaccharides | |
CN113151045A (en) | Novel lactobacillus plantarum isolated from tea tree leaves | |
JP2004154122A (en) | Lactic acid-fermented bean curd refuse and food containing the same, and method for producing the same | |
JP3091196B1 (en) | Method for producing pickles using lactic acid bacteria | |
BREMNER et al. | Effect of potassium sorbate on refrigerated storage of vacuum packed scallops | |
CN112471464A (en) | Preparation method of pickled hemp bamboo shoots | |
JPH0616696B2 (en) | How to improve the shelf life of pickles | |
JP2593781B2 (en) | Manufacturing method of salt-free fermented pickles | |
CN114668137A (en) | A kind of chili sauce with anti-acidity and taste and preparation method thereof | |
JP4216539B2 (en) | Ham production method | |
Yoo et al. | Antibacterial effect of glucose oxidase on growth of Pseudomonas fragi as related to pH | |
JP2009278964A (en) | Chitosan powder for salt-pickled food, salt-pickled food using the same and kimchi produced using the same | |
JPH01233204A (en) | Agent for improving growth of bean sprout | |
JPH0144296B2 (en) | ||
JP4623041B2 (en) | Beverage composition and method for producing the same | |
JP3066891B2 (en) | Food preservatives | |
JPH05308895A (en) | Processed fishery food and its production | |
KR100366855B1 (en) | Technical re-treatment of fermented Kimchi | |
CN116584605A (en) | A kind of food preservation enzyme and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |