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CN112237240A - Preparation technology of slow-release enteric microcapsule plant essential oil - Google Patents

Preparation technology of slow-release enteric microcapsule plant essential oil Download PDF

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Publication number
CN112237240A
CN112237240A CN201910640363.4A CN201910640363A CN112237240A CN 112237240 A CN112237240 A CN 112237240A CN 201910640363 A CN201910640363 A CN 201910640363A CN 112237240 A CN112237240 A CN 112237240A
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CN
China
Prior art keywords
essential oil
release
slow
plant essential
microcapsule
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Application number
CN201910640363.4A
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Chinese (zh)
Inventor
何蒙曦
陈朝文
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Chongqing Flavor Biotechnology Co ltd
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Chongqing Flavor Biotechnology Co ltd
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Priority to CN201910640363.4A priority Critical patent/CN112237240A/en
Publication of CN112237240A publication Critical patent/CN112237240A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/30Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention belongs to the technical field of plant essential oil preparation, and discloses a preparation technology of slow-release enteric microcapsule plant essential oil, wherein the plant essential oil adopts a structural form of an essential oil microcapsule, and the essential oil microcapsule is prepared in a double-coating mode and specifically comprises an inner coating and an outer coating; mixing acrylic resin II and acrylic resin III to form an inner coating material, and coating a plant essential oil core material to form an enteric slow-release pill; taking chitosan-Arabic gum as a composite capsule wall material as an outer capsule, and coating the composite capsule wall material outside the enteric sustained-release pill to form an essential oil microcapsule; in the double-coated essential oil microcapsule, the inner coating material is dissolved in intestinal solution but not in gastric juice, so that the microcapsule smoothly passes through the stomach to reach the intestinal tract, and stable slow release of the vegetable oil is carried out in the intestinal tract, thereby forming a good bacteriostatic effect on the intestinal tract; the outer capsule material has good thermal stability, thereby improving the controlled release and sustained release performance of the essential oil microcapsule.

Description

Preparation technology of slow-release enteric microcapsule plant essential oil
Technical Field
The invention belongs to the technical field of plant essential oil preparation, and particularly relates to a preparation technology of slow-release enteric microcapsule plant essential oil.
Background
The active ingredients in the natural plant essential oil are mostly phenol, aldehyde, terpenes and other substances, and the natural plant essential oil has the characteristics of poor stability (low melting point, easy volatilization, easy oxidation), strong irritation, peculiar smell and difficult storage; and as the concentration of the natural plant essential oil is higher, the pungent smell is stronger, so that the palatability of the feed is greatly influenced, and the feed intake is reduced; in addition, untreated essential oil is rapidly absorbed at the front ends of the stomach and the intestinal tract and cannot effectively reach the rear intestine to achieve the effects of bacteriostasis and the like, and particularly, carvacrol, thymol and other substances are easy to combine with albumin to generate an inactivation phenomenon;
the slow release technology is a technology for coating solid or liquid or gas by adopting natural, semisynthetic or synthetic polymer film-forming materials to form micro particles, and has the characteristics of slow release, taste masking, solidification of liquid components, enhancement of the stress resistance of the components, stomach passing (dissolution reduction), intestinal positioning release and good fluidity;
in conclusion, the animal edible essential oil with strong stability, good palatability and long positioning and bacteriostasis time can be prepared based on the slow release technology.
Disclosure of Invention
The invention aims to provide a preparation technology of slow-release enteric microcapsule plant essential oil, and aims to solve the problems that the existing natural plant essential oil is poor in stability and palatability and cannot be accurately positioned for bacteriostasis.
In order to achieve the purpose, the invention provides the following technical scheme:
1. the slow-release enteric microcapsule plant essential oil is in the structural form of an essential oil microcapsule, and the essential oil microcapsule is manufactured in a double-coating mode and specifically comprises an inner coating and an outer coating;
specifically, the method comprises the following steps: mixing acrylic resin II and acrylic resin III to form an inner coating material, and coating a plant essential oil core material to form an enteric slow-release pill; the chitosan-Arabic gum is taken as a composite capsule material to be an outer capsule, and the enteric sustained-release pill is coated with the chitosan-Arabic gum to form the essential oil microcapsule.
Preferably, the plant essential oil core material consists of an essential oil fixative, a synergistic raw material, a phagostimulant and plant base oil, wherein the synergistic raw material not only can generate a synergistic effect with the main components of the essential oil, but also can form an essential oil phagostimulant with the phagostimulant.
Specifically, the method comprises the following steps: the essential oil fixative mainly comprises vanillin, benzyl alcohol and benzyl benzoate; the synergistic raw materials mainly comprise p-cymene, limonene and pinene; the phagostimulant mainly comprises cordyceps peptide, tangerine peel oil, acetoin and cinnamaldehyde; it is worth mentioning that the cinnamaldehyde has the bacteriostatic effect and the food calling effect.
Preferably, the plant essential oil core material and the inner coating or the outer coating are coated by adopting a saturated aqueous solution method or a complex coacervation method.
2. The preparation technology of the slow-release enteric microcapsule plant essential oil comprises the following preparation steps:
a. mixing oregano oil, thyme oil, eugenol, vanillin, limonene, lemon essential oil, cinnamaldehyde, cordyceps peptide and acetoin to form a compound essential oil core material;
b. respectively preparing acrylic resin II and acrylic resin III aqueous solutions, mixing, placing in a constant-temperature water bath at 50 ℃, and uniformly stirring;
c. mixing the compound essential oil core material prepared in the step a into the solution prepared in the step b, carrying out high-speed homogenizing and emulsifying, simultaneously adding 10% acetic acid solution into the mixed solution, and adjusting the pH value to 3.8-4.0;
d. taking the mixed solution prepared in the step c out of the water bath environment, continuously stirring, and naturally cooling to 32-35 ℃;
e. d, putting the cooled mixed solution in the step d into an ice water environment, carrying out water bath cooling to reduce the temperature to below 10 ℃, simultaneously adding a 20% sodium hydroxide solution into the mixed solution, adjusting the pH value to 8-9, and continuously stirring to separate out the enteric-coated sustained-release pills; specifically, the grain diameter of the precipitated enteric-coated sustained-release pill is less than 2.5 mm;
f. respectively preparing 0.5 percent aqueous solution and 4 percent aqueous solution of chitosan and Arabic gum, and uniformly stirring in a constant-temperature water bath at 50-55 ℃;
g. taking the enteric-coated sustained-release pill and the solution prepared in the step f as raw materials, and repeating the operations of the step c, the step d and the step e to separate out the essential oil microcapsules;
compared with the prior art, the invention has the following beneficial effects:
(1) the essential oil microcapsule is prepared by adopting a double coating mode, wherein the inner coating is formed by mixing acrylic resin II and acrylic resin III and is dissolved in an intestinal solution, so that the microcapsule smoothly passes through the stomach to reach the intestinal tract, and the vegetable oil is stably released in the intestinal tract, thereby forming a good antibacterial effect on the intestinal tract; in addition, the outer capsule adopts chitosan and Arabic gum as composite capsule walls, and the encapsulation is stable, so that the essential oil microcapsule has good controlled release and slow release effects, and meanwhile, the composite capsule walls are non-toxic and harmless and have good biodegradation performance.
(2) The inner coating and the composite capsule wall have good slow release performance, the plant essential oil is slowly released in intestinal tracts, and the positioning bacteriostasis time is prolonged, so that the plant essential oil prepared by the invention has a more lasting effect.
(3) In the invention, the plant essential oil is also added with the essential oil fixative, thereby effectively delaying the release of the aroma of the essential oil and the volatilization of the essential oil, further improving the stability of the essential oil and being beneficial to the storage of the essential oil; meanwhile, the volatilization speed of the essential oil is reduced, and a certain slow release purpose is achieved.
(4) In order to solve the problem that the plant essential oil is poor in using taste, a certain essential oil phagostimulant is added, and the essential oil phagostimulant mainly comprises a synergistic raw material formed by cymene, limonene and pinene and a phagostimulant formed by cordyceps peptide, acetoin and cinnamaldehyde, so that the odor of the plant essential oil can be effectively improved, and the feeding is promoted.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
1. The invention provides a slow-release enteric microcapsule plant essential oil which adopts the structural form of an essential oil microcapsule, and the corresponding essential oil microcapsule is manufactured in a double-coating mode and specifically comprises an inner coating and an outer coating;
specifically, the method comprises the following steps: mixing acrylic resin II and acrylic resin III to form an inner coating material, and coating a plant essential oil core material to form an enteric slow-release pill: the formed inner coating material is difficult to dissolve in gastric juice, but can form a dissolution rate of 75-84.5% in intestinal solution, so that the plant essential oil coated inside the inner coating can be effectively released in the intestinal tract, and a good bacteriostatic action is formed on the intestinal tract.
Taking chitosan-Arabic gum as a composite capsule material as an outer capsule, and coating the composite capsule material outside the enteric sustained-release pill to form an essential oil microcapsule: the formed essential oil microcapsules have the encapsulation rate of 62-65%, the encapsulation effect is stable, and the composite capsule wall material formed by the chitosan-Arabic gum also has good biodegradation performance.
Preferably, the plant essential oil core material consists of an essential oil fixative, a synergistic raw material, a phagostimulant and plant base oil, wherein the synergistic raw material and the phagostimulant are matched to form an essential oil phagostimulant component;
further: (1) the fixative mainly comprises vanillin, benzyl benzoate and benzyl alcohol: the vanillin has the function of delaying the fragrance release of the essential oil, and the higher the vanillin amount is, the longer the fragrance retention time of the essential oil is; the benzyl benzoate has better stability, and achieves the purpose of slow release while reducing the volatilization of effective components and increasing the storage time of essential oil;
(2) the synergistic raw materials mainly comprise p-cymene, limonene and pinene: wherein, cymene, limonene, pinene and the like are auxiliary components of various essential oils and have synergistic effect on the main components of the essential oils;
(3) the phagostimulant mainly comprises cordyceps peptide, acetoin and cinnamaldehyde: wherein the Cordyceps peptide has effects of tranquilizing, relieving pain, inducing sleep, promoting ingestion, prolonging gastrointestinal peristalsis, and stimulating gastrointestinal hormone release; acetoin is one of the components in natural cream and has strong food calling effect on animals; the cinnamaldehyde has obvious antibacterial effect and can promote animal feeding.
Preferably, the plant essential oil core material and the inner coating or the outer coating are coated by adopting a saturated aqueous solution method or a complex coacervation method.
2. Aiming at the plant essential oil and the essential oil microcapsule, the preparation technology comprises the following preparation steps:
a. mixing oregano oil, thyme oil, eugenol, vanillin, lemon essential oil, cinnamaldehyde, cordyceps peptide and acetoin to form a compound essential oil core material;
b. respectively preparing acrylic resin II and acrylic resin III aqueous solutions, mixing, placing in a constant-temperature water bath at 50 ℃, and uniformly stirring;
c. mixing the compound essential oil core material prepared in the step a into the solution prepared in the step b, carrying out high-speed homogenizing and emulsifying, and simultaneously adjusting the pH value to 3.8-4.0;
specifically, the method comprises the following steps: in the process of adjusting the pH value, a 10% acetic acid solution is added into the mixed solution for adjustment, and meanwhile, the uniformity of mixing among the solutions is required to be ensured;
d. taking the mixed solution prepared in the step c out of the water bath environment, continuously stirring, and naturally cooling to 32-35 ℃;
e. d, putting the cooled mixed solution in the step d into an ice water environment, carrying out water bath cooling to reduce the temperature to below 10 ℃, simultaneously adjusting the pH value to 8-9, and continuously stirring to separate out the enteric-coated sustained-release pill;
specifically, the method comprises the following steps: in the process of adjusting the pH value, a 20% sodium hydroxide solution is added into the mixed solution for adjustment;
further: the grain diameter of the precipitated enteric-coated sustained-release pill is less than 2.5mm, so that the enteric-coated sustained-release pill can be stably coated in the outer capsule;
f. respectively preparing 0.5 percent aqueous solution and 4 percent aqueous solution of chitosan and Arabic gum, and uniformly stirring in a constant-temperature water bath at 50-55 ℃;
g. taking the enteric-coated sustained-release pill and the solution prepared in the step f as raw materials, and repeating the operations of the step c, the step d and the step e to separate out the essential oil microcapsules;
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. The slow-release enteric microcapsule plant essential oil is characterized in that: the plant essential oil adopts the structural form of essential oil microcapsules, and the essential oil microcapsules are manufactured in a double-coating mode and specifically comprise an inner coating and an outer coating;
mixing acrylic resin II and acrylic resin III to form an inner coating material, and coating a plant essential oil core material to form an enteric slow-release pill;
the chitosan-Arabic gum is taken as a composite capsule material to be an outer capsule, and the enteric sustained-release pill is coated with the chitosan-Arabic gum to form the essential oil microcapsule.
2. The slow-release enteric microcapsule plant essential oil according to claim 1, characterized in that: the plant essential oil core material consists of plant base oil, a fragrance fixative, a synergistic raw material and a phagostimulant; the synergistic raw materials not only can generate synergistic effect with the essential oil main components, but also can form the essential oil phagostimulant components together with the phagostimulant.
3. The slow-release enteric microcapsule plant essential oil according to claim 2, characterized in that: the fixative mainly comprises vanillin, benzyl benzoate and benzyl alcohol.
4. The slow-release enteric microcapsule plant essential oil according to claim 2, characterized in that: the synergistic raw materials mainly comprise p-cymene, limonene and pinene.
5. The slow-release enteric microcapsule plant essential oil according to claim 2, characterized in that: the phagostimulant mainly comprises cordyceps peptide, tangerine peel oil, acetoin and cinnamaldehyde.
6. The slow-release enteric microcapsule plant essential oil according to claim 1, characterized in that: the plant essential oil core material and the inner coating or the outer coating are coated by adopting a saturated aqueous solution method or a complex coacervation method.
7. The technology for preparing the slow-release enteric microcapsule plant essential oil according to any claim, characterized by comprising the following preparation steps:
a. mixing oregano oil, thyme oil, eugenol, vanillin, lemon essential oil, limonene, cinnamaldehyde, cordyceps peptide and acetoin to form a compound essential oil core material;
b. respectively preparing acrylic resin II and acrylic resin III aqueous solutions, mixing, placing in a constant-temperature water bath at 50 ℃, and uniformly stirring;
c. mixing the compound essential oil core material prepared in the step a into the solution prepared in the step b, carrying out high-speed homogenizing and emulsifying, and simultaneously adjusting the pH value to 3.8-4.0;
d. taking the mixed solution prepared in the step c out of the water bath environment, continuously stirring, and naturally cooling to 32-35 ℃;
e. d, putting the cooled mixed solution in the step d into an ice water environment, carrying out water bath cooling to reduce the temperature to below 10 ℃, simultaneously adjusting the pH value to 8-9, and continuously stirring to separate out the enteric-coated sustained-release pill;
f. respectively preparing 0.5 percent aqueous solution and 4 percent aqueous solution of chitosan and Arabic gum, and uniformly stirring in a constant-temperature water bath at 50-55 ℃;
g. taking the enteric-coated sustained-release pill and the solution prepared in the step f as raw materials, and repeating the operations of the step c, the step d and the step e to realize the precipitation of the essential oil microcapsules.
8. The technology for preparing the slow-release enteric microcapsule plant essential oil according to claim 7, characterized in that: the pH value adjustment in the step c adopts a mode of adding 10% acetic acid solution; and e, adjusting the pH value in the step e by adding 20% sodium hydroxide solution.
9. The technology for preparing the slow-release enteric microcapsule plant essential oil according to claim 7, characterized in that: and e, the grain diameter of the enteric-coated sustained-release pill precipitated in the step e is less than 2.5 mm.
CN201910640363.4A 2019-07-16 2019-07-16 Preparation technology of slow-release enteric microcapsule plant essential oil Pending CN112237240A (en)

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CN102511909A (en) * 2012-01-07 2012-06-27 中国海洋大学 Microcapsule
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CN104705533A (en) * 2015-04-14 2015-06-17 上海美农生物科技股份有限公司 High-stability pig plant essential oil additive as well as preparation method and application thereof
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004006896A1 (en) * 2002-07-16 2004-01-22 Warner-Lambert Company Llc Oral care capsules
CN101269051A (en) * 2008-05-08 2008-09-24 武汉工业学院 Rumen-passed choline chloride microcapsules and its preparation process
CN102511909A (en) * 2012-01-07 2012-06-27 中国海洋大学 Microcapsule
CN103752236A (en) * 2014-01-09 2014-04-30 陕西科技大学 Preparation method of double-layer wrapped essence micro-capsule
CN104353401A (en) * 2014-10-24 2015-02-18 苏州香满庭植物科技有限公司 Complex coacervation multicore plant essential oil slow-release microcapsule as well as preparation method and application thereof
CN104705533A (en) * 2015-04-14 2015-06-17 上海美农生物科技股份有限公司 High-stability pig plant essential oil additive as well as preparation method and application thereof
WO2017175225A1 (en) * 2016-04-06 2017-10-12 Botanocap Ltd. Spoilage retardant multilayer materials containing food safe adhesives
CN107773554A (en) * 2017-09-07 2018-03-09 华南农业大学 A kind of ivermectin slow-releasing microcapsule and its preparation method and application

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Application publication date: 20210119