CN112206905A

CN112206905A - Method and device for ultramicro treatment of bioactive substances

Info

Publication number: CN112206905A
Application number: CN202010928230.XA
Authority: CN
Inventors: 章琳; 袁士豪
Original assignee: Hangzhou Dianzi University
Current assignee: Hangzhou Dianzi University
Priority date: 2020-09-07
Filing date: 2020-09-07
Publication date: 2021-01-12

Abstract

The present invention relates to a method and an apparatus for ultramicro-treating a biologically active substance. Part of evaporated nitrogen from the first-stage mechanical pulverizer is filtered, converged with nitrogen from the top of the solid particle separation tank and nitrogen from the top of the second-stage centrifugal pulverizer, and then sent to an ambient air cooler to reduce the ambient temperature; the other part conveys the crushed solid particles into a solid particle separating tank. The nitrogen after cooling the ambient air is passed through a nitrogen solids filter to remove contaminant particles. Then, circulating nitrogen is sent into a coarse particle sieve through a blower and a nitrogen buffer tank, and qualified solid particles are sent into a second-stage centrifugal crusher, so that the cyclic utilization of the nitrogen is realized. The invention takes nitrogen after liquid nitrogen vaporization as a transport medium of the bioactive ultramicro powder; the circulation of air in the production space is accelerated, prevents that the dust from gathering, reduces the explosion danger, reduces the production environment and to operating personnel's health injury.

Description

Method and device for ultramicro treatment of bioactive substances

Technical Field

The invention belongs to the technical field of biological active substance ultramicro treatment, and relates to a biological active substance ultramicro treatment method and a biological active substance ultramicro treatment device.

Background

Currently, cryogenic comminution techniques are one of the alternatives to material comminution by efficiently and rapidly comminuting the material to fine particles without compromising the biological activity of the material. However, the existing low-temperature crushing equipment in a liquid nitrogen temperature area is usually small in scale and is suitable for small-batch production in a laboratory. The large-scale low-temperature crushing equipment generally has the working temperature not lower than-60 ℃ (is not suitable for crushing certain bioactive substances), and has dust pollution and explosion hidden danger in the production environment. The invention relates to a biological active substance ultramicro treatment technology and a device, which utilizes liquid nitrogen to quickly freeze the biological active substance, and the nitrogen evaporated by the liquid nitrogen can be used for (1) producing an environment cooling cold source; (2) a delivery medium for the bioactive superfine powder; (3) the air circulation in the production space is accelerated, and the dust accumulation is prevented.

Disclosure of Invention

The invention aims to provide a method and a device for the ultramicro treatment of a bioactive substance, which realize the production of ultramicro bioactive particles on the basis of not destroying the biological activity of the substance.

A method for ultramicro-treating a bioactive substance, comprising the steps of:

step one, storing biomass active raw materials loaded by a raw material truck in a material supply tank through a raw material conveyor belt so as to prepare for production requirements. Before the device is started, the nitrogen of the pipeline is required to be used for purging the pipeline of the system, so that impurity particles in the pipeline are removed.

And step two, crushing the biological active material which is subjected to liquid nitrogen quick freezing by a first-stage mechanical crusher, primarily crushing the frozen and embrittled biological active material into solid particles with the diameter of less than 0.6mm, and conveying the biological active solid particles into a solid particle separation tank by evaporating cold nitrogen.

And step three, primarily screening the tiny solid particles by a solid particle separation tank, recovering the solid particles with the particle diameter larger than 0.1mm, and inputting the solid particles into the first-stage mechanical pulverizer again for pulverizing.

At the moment, one part of cold nitrogen generated after liquid nitrogen evaporation of the first-stage mechanical pulverizer is used for conveying bioactive solid particles into the solid particle separation tank, and the other part of cold nitrogen is filtered and then converged with nitrogen at the top of the solid particle separation tank and nitrogen discharged by the second-stage centrifugal pulverizer to serve as a production environment cooling cold source and be used for reducing the temperature of a production workshop in a high-temperature season.

And step four, conveying the micro particles separated by the solid particle separating tank into a second-stage centrifugal crusher through nitrogen for secondary crushing. And screening the crushed micro solid particles through a product particle sieve, and directly packaging the solid particles with the diameter of less than 50 mu m as a product. And returning the solid particles with unqualified diameters to the first-stage mechanical pulverizer to be pulverized, and repeating the second step to the fourth step until all raw materials are pulverized into tiny solid particles.

At the moment, part of cold nitrogen after the liquid nitrogen of the first-stage mechanical pulverizer is evaporated, and nitrogen at the top of the solid particle separation tank and nitrogen discharged by the second-stage centrifugal pulverizer are converged to serve as a production environment cooling cold source.

The invention adopts two-stage crushing, and comprises the following biological active substance ultramicro treatment devices:

the device comprises a material supply tank, a raw material processing tank, a raw material preparation tank, a raw material quick freezing tank, a liquid nitrogen storage tank, a first-stage mechanical crusher, a solid particle separation tank, a coarse particle sieve, an ambient air cooler, a second-stage centrifugal crusher, a product particle sieve, an oversized particle collection barrel, a nitrogen buffer tank, a nitrogen solid particle filter and a dust collection barrel;

the material supply tank is connected with the raw material processing tank, and the raw material processing tank is connected with the raw material preparation tank; the raw material preparation tank is connected with the raw material quick freezing tank, and the raw material preparation tank quantitatively supplies biomass active raw materials for the raw material quick freezing tank. The liquid nitrogen storage tank is connected with the raw material quick-freezing tank through the liquid nitrogen pump, and after the raw material preparation tank adds the raw material for the raw material quick-freezing tank, the liquid nitrogen storage tank supplies liquid nitrogen for the raw material quick-freezing tank through the liquid nitrogen pump.

The discharge hole of the raw material quick freezing tank is connected with the feed inlet of the first-stage mechanical pulverizer. The powder outlet of the first-stage mechanical pulverizer is connected with the inlet of the solid particle separating tank, the outlet at the bottom of the solid particle separating tank is connected with the coarse particle sieve, and the gas outlet of the first-stage mechanical pulverizer and the gas outlet of the solid particle separating tank are both connected with the cold end inlet of the ambient air cooler. The feed inlet of the coarse particle sieve is connected with the discharge outlet of the first-stage mechanical crusher, the discharge outlet of the coarse particle sieve is connected with the feed inlet of the second-stage centrifugal crusher, and the nitrogen inlet of the coarse particle sieve is connected with the outlet of the nitrogen buffer tank.

And the discharge port of the second-stage centrifugal crusher is connected with the feed port of the product particle sieve. The discharge port of the product particle sieve is respectively connected with the feed port of the product packaging machine and the feed port of the oversize particle collecting barrel. The discharge hole of the oversize particle collecting barrel is connected with the feed inlet of the first-stage mechanical pulverizer. The air outlet of the second-stage centrifugal crusher is connected with the cold end inlet of the ambient air cooler.

The outlet of the cold end of the ambient air cooler is connected with the inlet of a nitrogen solid particle filter, the bottom of the nitrogen solid particle filter is provided with a dust collecting barrel, and the outlet of the dust collecting barrel is connected with a first-stage mechanical crusher; the outlet of the nitrogen solid particle filter is connected with the inlet of a nitrogen buffer tank through a nitrogen blower.

The material conveying of the material supply tank is realized through the material conveying belt and the material truck.

The material supply tank is connected with the raw material processing tank through a clean pipeline.

And a raw material quick-freezing tank safety valve is arranged at the top of the raw material quick-freezing tank.

The invention uses liquid nitrogen to quickly freeze and embrittle the bioactive substance, then crushes the bioactive substance by a centrifugal crusher, and finally directly packages the qualified ultramicro powder by screening. The invention recycles the liquid nitrogen used for freezing: (1) taking nitrogen vaporized by liquid nitrogen as a transport medium of the bioactive ultramicro powder; (2) the cold nitrogen after the liquid nitrogen evaporation is used as a production environment cooling cold source, and the cold energy of the liquid nitrogen is recycled; (3) the circulation of air in the production space can be accelerated to part circulation nitrogen gas, prevents that the dust from gathering, reduces the explosion danger, reduces the production environment and to operating personnel's health injury.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Detailed Description

As shown in FIG. 1, a two-stage pulverization system is used as a biological active substance ultrafine treatment apparatus. The material feed tank 24 is located at the top level of the apparatus in this embodiment. The material truck 1 transports the material to the material supply tank 24 via the material conveyor 2. The processing raw material tank 3 is spatially positioned at the lower layer of the material supply tank 24 and is connected with the material supply tank through a clean pipeline. The lower layer of the processing raw material tank 3 is connected with a raw material preparation tank 4. The layers are interconnected by a ladder 23.

The material supply tank 3 intermittently receives a material supply from the material supply tank 24 during the production process. The treatment material tank 3 feeds a material preparation tank 4. The raw material preparation tank 4 is connected to a raw material flash freezing tank 7 arranged above a horizontal ground. Meanwhile, a liquid nitrogen storage tank 5 is connected with the raw material quick freezing tank 7 through a liquid nitrogen pump 6, and liquid nitrogen is input into the raw material quick freezing tank 7. The raw material preparation tank 4 supplies biomass active raw material quantitatively to the raw material flash tank 7 (the amount of raw material entering the raw material flash tank 7 is constant each time). After the raw material preparation tank 4 is used for adding raw materials into the raw material quick freezing tank 7, the liquid nitrogen storage tank 5 supplements liquid nitrogen for the raw material quick freezing tank 7 through the liquid nitrogen pump 6. The biomass active raw material is fully mixed with liquid nitrogen in a raw material quick freezing tank 7 and is rapidly cooled to a liquid nitrogen temperature zone.

The discharge hole of the raw material quick freezing tank 7 is connected with the feed inlet of the first-stage mechanical crusher 9. The powder outlet of the first-stage mechanical pulverizer is connected with the inlet of the solid particle separating tank 10. The frozen and embrittled biomass active raw materials are input into a first-stage mechanical crusher 9 through self gravity and nitrogen pressure after liquid nitrogen evaporation. The raw material pulverized by the first-stage mechanical pulverizer 9 is conveyed into a solid particle separation tank 10 by using evaporated nitrogen as a conveying medium.

Part of the nitrogen gas from the first mechanical pulverizer 9 is filtered and then combined with the nitrogen gas from the top of the solid particle separation tank and the nitrogen gas from the second centrifugal pulverizer, and the nitrogen gas is sent to an ambient air cooler 19, and the other part of the nitrogen gas is sent to the solid particle separation tank 10 after being pulverized. The raw material quick-freezing tank safety valve is arranged at the upper part of the raw material quick-freezing tank 7 and is used for preventing nitrogen overpressure of the raw material quick-freezing tank 7.

The outlet at the bottom of the solid particle separating tank 10 is connected with a coarse particle sieve 11. The coarse particle sieve 11 is respectively connected with a discharge hole of the first-stage mechanical crusher 9, an outlet of the circulating nitrogen buffer tank 22 and a feed hole of the second-stage centrifugal crusher 12. The gas-solid mixture is separated from the nitrogen and the solid particles in the solid particle separating tank 10. The separated nitrogen is discharged to the ambient air cooler 19 through the top outlet of the solid particles separating tank 10. The solid particles at the bottom are screened by a coarse particle screen 11, the large particles are returned to the first-stage mechanical crusher 9 for crushing, and the qualified particles are conveyed into the second-stage centrifugal crusher 12 by conveying nitrogen from a nitrogen buffer tank 22.

The discharge hole of the second-stage centrifugal crusher 12 is connected with the feed hole of the product particle sieve 13. The discharge hole of the particle sieve 13 is respectively connected with the feed hole of the product packaging machine 15 and the feed hole of the oversize particle collecting barrel 14. The discharge hole of the oversize particle collecting barrel 14 is connected with the feed hole of the first-stage mechanical crusher 9. The oversized particles are further crushed into micron-sized particles in the second stage centrifugal crusher 12 and separation of nitrogen and solid particles is achieved. The separated nitrogen gas is discharged from the top outlet of the second stage centrifugal crusher 12 to the ambient air cooler 19.

After being screened by the product particle sieve 13, the solid particles with qualified sizes enter the product packaging machine 15 for packaging, and after the packaging is finished, the solid particles are conveyed to the product conveying forklift 17 through the product conveying belt 16 for transportation. The solid particles with unqualified sizes enter the oversized particle collecting barrel 14 and return to the first-stage mechanical crusher 9 for crushing.

And an inlet at the cold end of the ambient air cooler 19 is respectively connected with a nitrogen outlet at the top of the first-stage mechanical pulverizer 9, a nitrogen outlet at the top of the solid particle separation tank 10 and a nitrogen outlet at the top of the second-stage centrifugal pulverizer 12. An outlet of the cold end of the ambient air cooler 19 is connected with an inlet of a nitrogen solid particle filter 20, and the bottom of the nitrogen solid particle filter 20 is provided with a dust collecting barrel 18. The outlet of the dust collecting barrel 18 is connected with the first-stage mechanical crusher 9. The outlet of the nitrogen solid particle filter 20 is connected with the inlet of a nitrogen blower 21, and the outlet of the nitrogen blower 21 is connected with the inlet of a nitrogen buffer tank 22. The outlet of the nitrogen buffer tank 22 is connected with the nitrogen inlet of the coarse particle sieve 11.

The device is based on the following biological active substance ultramicro treatment method, and the specific steps are as follows:

step one, the raw material truck loaded biomass active material is stored in the material supply tank 24 by the raw material conveyor belt for the production demand. Before the device is started, the nitrogen of the pipeline is required to be used for purging the pipeline of the system, so that impurity particles in the pipeline are removed.

And step two, crushing the biological active material which is subjected to the liquid nitrogen quick freezing by a first-stage mechanical crusher 9, primarily crushing the frozen and embrittled biological active material into solid particles with the diameter of less than 0.6mm, and conveying the biological active solid particles into a solid particle separation tank 10 by evaporating cold nitrogen.

And step three, primarily screening the tiny solid particles by the solid particle separating tank 10, recovering the solid particles with the particle diameter larger than 0.1mm, and inputting the solid particles into the first-stage mechanical pulverizer 9 again for re-pulverization.

At the moment, one part of cold nitrogen gas after liquid nitrogen evaporation is used for conveying bioactive solid particles into the solid particle separation tank 10, and the other part of cold nitrogen gas after filtration is combined with nitrogen gas at the top of the solid particle separation tank and nitrogen gas discharged by a second-stage centrifugal crusher to serve as a production environment cooling cold source and be used for reducing the temperature of a production workshop in a high-temperature season; and step four, delivering the micro particles separated by the solid particle separating tank 10 into a second-stage centrifugal crusher 12 through nitrogen for secondary crushing. The crushed micro solid particles are screened by a product particle screen 13, and the solid particles with the diameter less than 50 mu m are directly packaged as products. And returning the solid particles with unqualified diameters to the first-stage mechanical pulverizer to be pulverized, and repeating the second step to the fourth step until all raw materials are pulverized into tiny solid particles.

Nitrogen gas at the top of the first-stage mechanical pulverizer 9, nitrogen gas at the top of the solid particle separation tank 10 and nitrogen gas discharged by the second-stage centrifugal pulverizer 12 are converged, one part of the converged nitrogen gas is used as a production environment cooling cold source, and the other part of the converged nitrogen gas is used as purge gas, so that air circulation in a production workshop is accelerated to prevent dust accumulation.

The invention makes full use of the evaporated nitrogen in the crushing process. Part of the evaporated nitrogen from the first mechanical pulverizer 9 is filtered, merged with nitrogen from the top of the solid particle separation tank 10 and nitrogen from the top of the second centrifugal pulverizer 12, and then sent to an ambient air cooler 19 for reducing the ambient temperature; the other part delivers the pulverized solid particles to the solid particle separating tank 10. The nitrogen after cooling the ambient air is passed through a nitrogen solids filter 20 to remove contaminant particles. Then, the circulating nitrogen is sent into the coarse particle sieve 11 through a blower and a nitrogen buffer tank, and the qualified solid particles are sent into the second-stage centrifugal crusher 12, so that the cyclic utilization of the nitrogen is realized. And the nitrogen at the outlet part of the blower directly enters the production environment, so that the circulation of ambient air is accelerated, and the accumulation of dust is prevented.

Claims

1. a biologically active substance ultramicron processing method, is characterized in that: comprise the steps:

Step 1. Store the biomass active raw material loaded by the raw material truck in the material supply tank through the raw material conveyor belt to prepare for the production demand; before the device is started, it is necessary to use the pipeline nitrogen to purge the system pipeline to remove the impurity particles in the pipeline;

Step 2. The biologically active material after being rapidly frozen by liquid nitrogen is firstly pulverized by the first-stage mechanical pulverizer, and the frozen embrittled biologically active material is initially pulverized into solid particles with a diameter of less than 0.6 mm, and the biological The active solid particles are transported into the solid particle separation tank;

Step 3: Preliminary screening of tiny solid particles in the solid particle separation tank, solid particles with a particle diameter greater than 0.1 mm will be recovered and re-entered into the first-stage mechanical pulverizer for further pulverization;

At this time, part of the cold nitrogen evaporated from the liquid nitrogen of the first-stage mechanical pulverizer is used to transport the biologically active solid particles into the solid particle separation tank, and the other part is filtered with the nitrogen at the top of the solid particle separation tank, and the second-stage centrifugal pulverizer discharges nitrogen. Confluence, as a cooling source for the production environment, to reduce the temperature of the production workshop in the high temperature season;

Step 4. The tiny particles separated by the solid particle separation tank are transported into the second-stage centrifugal pulverizer for secondary pulverization through nitrogen; after pulverization, the tiny solid particles are screened through the product particle sieve, and the solid particles with a diameter of less than 50 μm are directly packaged as products If the diameter of the unqualified solid particles is returned to the first-level mechanical pulverizer for pulverization, repeat steps 2 to 4 until all raw materials are pulverized to tiny solid particles;

At this time, part of the cold nitrogen evaporated from the liquid nitrogen in the first-stage mechanical pulverizer, the nitrogen at the top of the solid particle separation tank, and the nitrogen discharged from the second-stage centrifugal pulverizer are combined to serve as a cooling source for the production environment.

2. The device for ultramicronization of biologically active substances used in the method of claim 1, characterized in that: a two-stage pulverization is adopted, including a material supply tank, a processing raw material tank, a raw material preparation tank, a raw material snap-freezing tank, and a liquid nitrogen storage tank. tank, first stage mechanical pulverizer, solid particle separation tank, coarse particle screen, ambient air cooler, second stage centrifugal pulverizer, product particle screen, oversized particle collection drum, nitrogen buffer tank, nitrogen solid particle filter and Dust collection bucket;

The material supply tank is connected with the processing raw material tank, and the processing raw material tank is connected with the raw material preparation tank; the raw material preparation tank is connected with the raw material freezing tank, and the raw material preparation tank is the raw material freezing tank for quantitatively supplying biomass active raw materials; the liquid nitrogen storage tank passes through the liquid nitrogen storage tank. The nitrogen pump is connected to the raw material freezing tank. After the raw material preparation tank is used to add raw materials to the raw material freezing tank, the liquid nitrogen storage tank is supplemented with liquid nitrogen for the raw material freezing tank through the liquid nitrogen pump;

The outlet of the raw material freezing tank is connected to the inlet of the first-stage mechanical pulverizer; the powder outlet of the first-stage mechanical pulverizer is connected to the inlet of the solid particle separation tank, the bottom outlet of the solid particle separation tank is connected to the coarse particle sieve, the first The air outlet of the first-stage mechanical pulverizer and the air outlet of the solid particle separation tank are connected to the cold end inlet of the ambient air cooler; the feed port of the coarse particle sieve is connected to the discharge port of the first-stage mechanical pulverizer, and the discharge port of the coarse particle sieve is connected to the second stage. The feed inlet of the centrifugal pulverizer, the nitrogen inlet of the coarse particle sieve is connected to the outlet of the nitrogen buffer tank;

The outlet of the second-stage centrifugal pulverizer is connected to the inlet of the product particle sieve; the outlet of the product particle sieve is respectively connected to the inlet of the product packaging machine and the inlet of the oversized particle collection bucket; The material port is connected to the feed port of the first-stage mechanical pulverizer; the air outlet of the second-stage centrifugal pulverizer is connected to the cold end inlet of the ambient air cooler;

The outlet of the cold end of the ambient air cooler is connected to the inlet of the nitrogen solid particle filter, a dust collection bucket is arranged at the bottom of the nitrogen solid particle filter, and the outlet of the dust collection bucket is connected to the first-stage mechanical pulverizer; the outlet of the nitrogen solid particle filter passes through a nitrogen blower Even the nitrogen buffer tank inlet.

3 . The device for ultramicronization of biologically active substances according to claim 2 , wherein the raw material of the material supply tank is conveyed through a material conveyor belt and a raw material truck. 4 .

4. The apparatus for ultramicronization of biologically active substances according to claim 2, wherein the material supply tank is connected to the processing raw material tank through a clean pipeline.

5. The apparatus for ultramicronization of biologically active substances as claimed in claim 2, wherein the top of the raw material quick-freezing tank is provided with a raw material quick-freezing tank safety valve.