CN112159781A - Lactobacillus plantarum, microbial agent and application thereof - Google Patents
Lactobacillus plantarum, microbial agent and application thereof Download PDFInfo
- Publication number
- CN112159781A CN112159781A CN202011153651.6A CN202011153651A CN112159781A CN 112159781 A CN112159781 A CN 112159781A CN 202011153651 A CN202011153651 A CN 202011153651A CN 112159781 A CN112159781 A CN 112159781A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- skin
- gdmcc
- collagen content
- plantarum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 54
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 54
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 54
- 230000000813 microbial effect Effects 0.000 title claims abstract description 13
- 102000008186 Collagen Human genes 0.000 claims abstract description 21
- 108010035532 Collagen Proteins 0.000 claims abstract description 21
- 229920001436 collagen Polymers 0.000 claims abstract description 21
- 244000005700 microbiome Species 0.000 claims abstract description 4
- 244000164595 Lactobacillus plantarum subsp plantarum Species 0.000 claims abstract 2
- 235000012523 Lactobacillus plantarum subsp plantarum Nutrition 0.000 claims abstract 2
- 235000013305 food Nutrition 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000002537 cosmetic Substances 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 235000013361 beverage Nutrition 0.000 claims description 2
- 235000013365 dairy product Nutrition 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 235000013311 vegetables Nutrition 0.000 claims description 2
- 208000002874 Acne Vulgaris Diseases 0.000 abstract description 7
- 206010000496 acne Diseases 0.000 abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 4
- 230000003078 antioxidant effect Effects 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 241000192125 Firmicutes Species 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 235000015140 cultured milk Nutrition 0.000 description 4
- 230000009759 skin aging Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- 241000304886 Bacilli Species 0.000 description 2
- 229920002444 Exopolysaccharide Polymers 0.000 description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 description 2
- 108700016172 Glutathione peroxidases Proteins 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- 108010059993 Vancomycin Proteins 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 229940118019 malondialdehyde Drugs 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000006872 mrs medium Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 235000021110 pickles Nutrition 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 2
- 229960003165 vancomycin Drugs 0.000 description 2
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 108030001720 Bontoxilysin Proteins 0.000 description 1
- 241000186427 Cutibacterium acnes Species 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 108010017842 Telomerase Proteins 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 229940053031 botulinum toxin Drugs 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002910 effect on acne Effects 0.000 description 1
- 235000021001 fermented dairy product Nutrition 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940050410 gluconate Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- -1 oxygen free radical Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Nutrition Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Birds (AREA)
- Molecular Biology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses Lactobacillus plantarum, the strain name of which is Lactobacillus plantarum subsp.plantarum FLPL135, the thallus is short rod-shaped gram-positive bacteria, the bacterial colony is milky white, shows smooth and neat, is stored in Guangdong province microbial strain storage center, and the storage number is GDMCC No: 61028. the invention belongs to the technical field of microorganisms, and provides lactobacillus plantarum GDMCC No: 61028 has strong antioxidant effect, can remarkably increase collagen content in aged skin, and has remarkable inhibiting effect on acne bacillus.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and relates to a novel Lactobacillus plantarum subsp.
Background
The lactobacillus plantarum belongs to one of lactic acid bacteria, is gram-positive, does not produce spores, is anaerobic or facultative anaerobic, is in a straight rod shape, is in a single, paired or chain-forming shape, belongs to homofermentation and chemotropy heterogeneous lactic acid bacteria, can ferment gluconate or pentose, has lactic acid as the most fermentation end product, reaches more than 85 percent, and can produce special lactobacillus in the propagation process. The lactobacillus plantarum has good adaptability and can live in various environments, different strains have different life habits, and the lactobacillus plantarum is the only microorganism which can live in various ecological environments. Comparative genomics analysis shows that the lactobacillus plantarum is in a nomadic life style in nature. The adaptability of the bacteria determines the wide distribution of the bacteria, including intestinal tracts of mammals, saliva, breast milk, fermented products (pickles, wine, traditional fermented dairy products) and the like.
The skin is the largest organ of the human body and is also an important organ in which the body and the outside world are in direct contact. As the first line of defense of immune response, it is mainly used to protect the body from various physical, chemical and pathogenic biological damages. However, the skin is disturbed by factors such as external environment, self-aging and the like, and the skin morphology can be obviously changed after the skin is in middle age, which is characterized by skin thinning, elasticity reduction, wrinkle appearance, dryness, roughness and the like. Skin aging is associated with oxygen free radical damage, hypoimmunity, decreased telomerase activity, inflammatory infiltration, etc. Usually, the beauty-loved people areThere is a great risk in delaying skin aging, for example, in treating skin aging by surgical methods such as botulinum toxin injection, collagen subcutaneous injection, and skin tightening. However, the use of probiotics can perfectly avoid the defects and promote human health while beautifying. Lactobacillus plantarum is reported to have antioxidant capacity in its thallus and metabolites. Such as Lactobacillus plantarum C88, has high DPPH and hydroxyl radical scavenging ability, and can be used for treating H2O2Has strong tolerance capability, and can remarkably improve the activity of superoxide dismutase (SOD) in serum, improve the activity of glutathione peroxidase (GSH-PX) in liver and reduce the content of Malondialdehyde (MDA) after intervening in a D-galactose-induced aged mouse. Lactobacillus plantarum MA2 tolerates 2.0mM H2O2Has strong reducibility and lipid peroxidation inhibition capability, and the fermentation supernatant and the cell homogenate have glutathione peroxidase activity and superoxide dismutase activity. Lactobacillus plantarum metabolites, such as Exopolysaccharides (EPS), also have a corresponding free radical scavenging capacity. The lactobacillus plantarum is used as a natural antioxidant, and the strain resource of the lactobacillus plantarum needs to be further mined and expanded.
Disclosure of Invention
The invention identifies a new lactobacillus plantarum FLPL135 with the preservation number GDMCC No. 61028, which is an anaerobic gram-positive bacterium without producing spores, the bacterium body is in a long rod shape, and is in a white, round and smooth colony in an MRS agar plate culture medium. The lactobacillus plantarum GDMCC No: 61028 can be grown in MRS broth and are gram positive bacteria.
The invention provides Lactobacillus plantarum, the strain name of which is Lactobacillus plantarum subsp. 61028 and preservation date of 2020, 5/18.
The invention also provides a microbial agent for improving the content of collagen in skin, which comprises the lactobacillus plantarum.
Preferably, the microbial agent for increasing the collagen content in the skin comprises 0.01-2% of lactobacillus plantarum in percentage by mass.
In addition, the invention also provides a food for increasing the content of collagen in skin, which comprises the lactobacillus plantarum or comprises the microbial inoculant; the food comprises dairy products, vegetable products and beverage products.
In addition, the invention also provides a cosmetic product for increasing the content of collagen in skin, which comprises the lactobacillus plantarum or comprises the microbial inoculant.
In addition, the invention also provides application of the lactobacillus plantarum in preparing medicines, foods or cosmetics for improving the content of the collagen.
The lactobacillus plantarum GDMCC No: 61028 can significantly increase the water content and collagen content of aged skin, inhibit acne bacillus, and help maintain the youthful state of skin and reduce the occurrence of acne. Lactobacillus plantarum GDMCC No: 61028 can be used for preparing microbial preparation, food or cosmetic, and has effects of increasing collagen content in skin and delaying skin aging. The lactobacillus plantarum GDMCC No: 61028 has strong antioxidant effect, can remarkably increase collagen content in aged skin, and has remarkable inhibiting effect on acne bacillus.
Drawings
FIG. 1 Lactobacillus plantarum GDMCC No: 61028 effects on moisture content of aged mouse skin.
FIG. 2 is a staining pattern of collagen in mouse skin tissue.
FIG. 3 Lactobacillus plantarum GDMCC No: 61028 results of the effect on collagen content of aged mouse skin.
FIG. 4 Lactobacillus plantarum GDMCC No: 61028 results of inhibiting the growth of acne bacteria.
The Lactobacillus plantarum provided by the invention has the strain name of Lactobacillus plantarum subsp. 61028 and preservation date of 2020, 5/18.
Detailed Description
The present invention is further explained below with reference to examples, but the examples are only for illustrating the present invention, and the application range of the present invention is not limited in any way by these examples.
Example 1: lactobacillus plantarum GDMCC No: 61028 isolation and identification
Separating from pickle, crushing, diluting by gradient 10, 100, 1000 and 10000, taking 100 mu L of the diluted product, coating the 100 mu L of the diluted product on an MRS solid culture medium containing 1% of vancomycin, carrying out inversion culture at 37 ℃ for 24h, picking single colony, scribing on the MRS solid culture medium containing 1% of vancomycin, carrying out inversion culture at 37 ℃ for 24h, picking single colony, carrying out colony PCR, and carrying out sequencing comparison on PCR results.
The 16S rRNA base sequence of the isolated strain was analyzed and sequenced, and the sequence was as follows:
according to the identification results, the separated strain is confirmed to belong to Lactobacillus plantarum, Lactobacillus plantarum subsp. 61028.
Example 2: effect of Lactobacillus plantarum GDMCC61028 on moisture content of aged mouse skin
Lactobacillus plantarum GDMCC No: 61028 can significantly maintain the moisture content of the skin of an aging mouse. The mice of 17 months were gavaged 10 daily9CFU/mL Lactobacillus plantarum GDMCC No: 61028 the fermented milk of 100 μ L, after 3 months, that is, 20 months, the skin moisture content was measured by a skin moisture tester, and found to be 22.95%, andthere was essentially no significant difference in skin moisture content of 23.39% in young 4-month mice, whereas the moisture content was significantly increased compared to aged 20-month mice (20.69%), and the results are shown in fig. 1.
Example 3: effect of Lactobacillus plantarum GDMCC61028 on collagen content of aged mouse skin
Lactobacillus plantarum GDMCC No: 61028 the collagen content of the skin of aged mice is significantly maintained. The mice of 17 months were gavaged 10 daily9CFU/mL Lactobacillus plantarum GDMCC No: 61028, 100 μ L of fermented milk, and after 3 months, collagen staining was performed on skin samples, and 20-month mice were used as controls, as shown in fig. 2 and 3. FIG. 3 shows that the content of collagen is very significantly increased after the administration of Lactobacillus plantarum.
Example 4: lactobacillus plantarum GDMCC No: 61028 Effect on acne bacilli
Lactobacillus plantarum GDMCC No: 61028 inhibit the growth of acne bacilli. Selection of propionibacterium acnes ATCC No: 6919 indicator bacteria, adjusting the concentration of the bacterial suspension by turbidimetry to 1 × 107cfu/mL. Add 1mL of bacterial suspension to the solid medium and spread well with a sterile triangular glass spatula. The Oxford cup is clamped by a pair of tweezers and is placed in the solid culture medium coated with the bacterial suspension, and the Oxford cup is lightly pressed to be in good contact with the culture medium. 100 μ L of Lactobacillus plantarum GDMCC No: 61028 fermented milk and MRS medium supernatant, 10% skim milk and MRS medium as blank control. Putting the culture dish into an anaerobic incubator, culturing for 48h at 37 ℃, and finally measuring the diameter of the bacteriostatic transparent ring by using a vernier caliper. The results show that whether the lactobacillus plantarum GDMCC No: 61028 fermented milk and MRS culture supernatant can effectively inhibit the growth of acne bacillus, and there is no significant difference between them.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Sequence listing
<110> Shenzhen Qin sail science and technology Limited
<120> lactobacillus plantarum, microbial inoculum and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1476
<212> DNA
<213> Lactobacillus plantarum
<400> 1
gcccagtggg ggggtgctat acatgcagtc gaacgaactc tggtattgat tggtgcttgc 60
atcatgattt acatttgagt gagtggcgaa ctggtgagta acacgtggga aacctgccca 120
gaagcggggg ataacacctg gaaacagatg ctaataccgc ataacaactt ggaccgcatg 180
gtccgagttt gaaagatggc ttcggctatc acttttggat ggtcccgcgg cgtattagct 240
agatggtggg gtaacggctc accatggcaa tgatacgtag ccgacctgag agggtaatcg 300
gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc 360
cacaatggac gaaagtctga tggagcaacg ccgcgtgagt gaagaagggt ttcggctcgt 420
aaaactctgt tgttaaagaa gaacatatct gagagtaact gttcaggtat tgacggtatt 480
taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 540
gttgtccgga tttattgggc gtaaagcgag cgcaggcggt tttttaagtc tgatgtgaaa 600
gccttcggct caaccgaaga agtgcatcgg aaactgggaa acttgagtgc agaagaggac 660
agtggaactc catgtgtagc ggtgaaatgc gtagatatat ggaagaacac cagtggcgaa 720
ggcggctgtc tggtctgtaa ctgacgctga ggctcgaaag tatgggtagc aaacaggatt 780
agataccctg gtagtccata ccgtaaacga tgaatgctaa gtgttggagg gtttccgccc 840
ttcagtgctg cagctaacgc attaagcatt ccgcctgggg agtacggccg caaggctgaa 900
actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagct 960
acgcgaagaa ccttaccagg tcttgacata ctatgcaaat ctaagagatt agacgttccc 1020
ttcggggaca tggatacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080
gttaagtccc gcaacgagcg caacccttat tatcagttgc cagcattaag ttgggcactc 1140
tggtgagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200
cttatgacct gggctacaca cgtgctacaa tggatggtac aacgagttgc gaactcgcga 1260
gagtaagcta atctcttaaa gccattctca gttcggattg taggctgcaa ctcgcctaca 1320
tgaagtcgga atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc 1380
ttgtacacac cgcccgtcac accatgagag tttgtaacac ccaaagtcgg tggggtaacc 1440
ttttaggaac cagccgccta aggtgaacaa aaatgt 1476
Claims (6)
1. A lactobacillus plantarum strain characterized by: the strain is named as Lactobacillus plantarum subsp.plantarum FLPL135, is preserved in Guangdong province microorganism strain preservation center, and has the preservation number of GDMCC No: 61028.
2. a microbial agent for increasing collagen content in skin, which is characterized in that: comprising the Lactobacillus plantarum strain defined in claim 1.
3. The microbial agent for increasing collagen content in skin according to claim 2, wherein: the mass percentage of the lactobacillus plantarum in the microbial agent is 0.01-2%.
4. A food product for increasing collagen content in skin, comprising: comprising the lactobacillus plantarum of claim 1, or comprising the microbial inoculant of claim 2 or 3; the food product comprises dairy products, vegetable products and beverage products.
5. A cosmetic for increasing collagen content in skin, comprising: comprising the Lactobacillus plantarum of claim 1, or comprising the microbial inoculant of claim 2 or 3.
6. Use of lactobacillus plantarum according to claim 1 for the preparation of a medicament, food or cosmetic product increasing the collagen content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011153651.6A CN112159781A (en) | 2020-10-23 | 2020-10-23 | Lactobacillus plantarum, microbial agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011153651.6A CN112159781A (en) | 2020-10-23 | 2020-10-23 | Lactobacillus plantarum, microbial agent and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112159781A true CN112159781A (en) | 2021-01-01 |
Family
ID=73866234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011153651.6A Pending CN112159781A (en) | 2020-10-23 | 2020-10-23 | Lactobacillus plantarum, microbial agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112159781A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112920983A (en) * | 2021-04-27 | 2021-06-08 | 江南大学 | Lactobacillus plantarum capable of improving facial sensitive skin and repairing skin barrier |
| CN114574408A (en) * | 2022-05-06 | 2022-06-03 | 山东锦鲤生物工程有限公司 | Probiotic SEUNEU-107 and application thereof |
| CN114657107A (en) * | 2022-04-21 | 2022-06-24 | 青岛蔚蓝生物股份有限公司 | Lactobacillus plantarum with acne treatment effect and application thereof |
| CN114774326A (en) * | 2022-05-11 | 2022-07-22 | 广西爱生生命科技有限公司 | Lactobacillus plantarum and application thereof |
| CN116790425A (en) * | 2023-06-06 | 2023-09-22 | 扬州大学 | Lactobacillus plantarum and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013153358A1 (en) * | 2012-04-13 | 2013-10-17 | The University Of Manchester | Probiotic bacteria |
| CN105505814A (en) * | 2015-11-30 | 2016-04-20 | 南昌大学 | Lactobacillus plantarum capable of delaying aging |
| CN108125902A (en) * | 2018-01-08 | 2018-06-08 | 北京科拓恒通生物技术股份有限公司 | A kind of probiotic composition, facial treatment essence liquid and facial mask and preparation method thereof |
| CN110151672A (en) * | 2018-02-12 | 2019-08-23 | 景岳生物科技股份有限公司 | Application of a plant Lactobacillus GMNL-6 composition for skin maintenance |
| CN111465386A (en) * | 2017-11-16 | 2020-07-28 | Cj第一制糖株式会社 | Cosmetic composition for reducing skin wrinkles comprising lactobacillus plantarum culture |
| CN111601584A (en) * | 2017-11-16 | 2020-08-28 | Cj第一制糖株式会社 | Antibacterial cosmetic composition comprising Lactobacillus plantarum culture |
-
2020
- 2020-10-23 CN CN202011153651.6A patent/CN112159781A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013153358A1 (en) * | 2012-04-13 | 2013-10-17 | The University Of Manchester | Probiotic bacteria |
| CN105505814A (en) * | 2015-11-30 | 2016-04-20 | 南昌大学 | Lactobacillus plantarum capable of delaying aging |
| CN111465386A (en) * | 2017-11-16 | 2020-07-28 | Cj第一制糖株式会社 | Cosmetic composition for reducing skin wrinkles comprising lactobacillus plantarum culture |
| CN111601584A (en) * | 2017-11-16 | 2020-08-28 | Cj第一制糖株式会社 | Antibacterial cosmetic composition comprising Lactobacillus plantarum culture |
| CN108125902A (en) * | 2018-01-08 | 2018-06-08 | 北京科拓恒通生物技术股份有限公司 | A kind of probiotic composition, facial treatment essence liquid and facial mask and preparation method thereof |
| CN110151672A (en) * | 2018-02-12 | 2019-08-23 | 景岳生物科技股份有限公司 | Application of a plant Lactobacillus GMNL-6 composition for skin maintenance |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112920983A (en) * | 2021-04-27 | 2021-06-08 | 江南大学 | Lactobacillus plantarum capable of improving facial sensitive skin and repairing skin barrier |
| CN114657107A (en) * | 2022-04-21 | 2022-06-24 | 青岛蔚蓝生物股份有限公司 | Lactobacillus plantarum with acne treatment effect and application thereof |
| CN114657107B (en) * | 2022-04-21 | 2023-11-17 | 青岛蔚蓝生物股份有限公司 | Lactobacillus plantarum with acne treatment effect and application thereof |
| CN114574408A (en) * | 2022-05-06 | 2022-06-03 | 山东锦鲤生物工程有限公司 | Probiotic SEUNEU-107 and application thereof |
| CN114574408B (en) * | 2022-05-06 | 2022-07-22 | 山东锦鲤生物工程有限公司 | Probiotic SEUNEU-107 and application |
| CN114774326A (en) * | 2022-05-11 | 2022-07-22 | 广西爱生生命科技有限公司 | Lactobacillus plantarum and application thereof |
| CN116790425A (en) * | 2023-06-06 | 2023-09-22 | 扬州大学 | Lactobacillus plantarum and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112159781A (en) | Lactobacillus plantarum, microbial agent and application thereof | |
| Chin-Wen et al. | Identification and characterisation of lactic acid bacteria and yeasts isolated from kefir grains in Taiwan | |
| WO2022236935A1 (en) | Probiotic having effect of inhibiting growth of proteus mirabilis, and fermentation broth and use thereof | |
| KR101399712B1 (en) | Novel fermented milk product and use thereof | |
| CN103937709A (en) | Lactobacillus paracasei FM-LP-4 with acid resistance and high reduction activity and application thereof | |
| Guzel-Seydim et al. | Kefir and koumiss: microbiology and technology | |
| CN112442458B (en) | Lactobacillus salivarius TCI153, and application thereof and/or bacterium breaking liquid thereof in improving skin condition | |
| CN110129219B (en) | A strain of Pediococcus pentosaceus and its application | |
| CN108148789A (en) | A kind of Lactobacillus rhamnosus and its application in bacteriocin is prepared | |
| Behbahani et al. | Evaluation of probiotic, safety, and anti-pathogenic properties of Levilactobacillus brevis HL6, and its potential application as bio-preservatives in peach juice | |
| CN113980853B (en) | Lactic acid-producing lactococcus garvieae WBT0008 and application thereof | |
| CN107988115A (en) | Lactobacillus plantarum and its composite probiotics ferment liquid and preparation method | |
| CN105400726A (en) | Human-derived lactobacillus acidophilus resisting gastrointestinal tract stress and application thereof | |
| CN111944712A (en) | Lactobacillus plantarum with excellent alcohol tolerance and application thereof | |
| CN114292779A (en) | Lactobacillus paracasei freeze-dried powder, application and preparation method thereof | |
| Adelekan et al. | Antioxidant activities of exopolysaccharides produced by lactic acid bacteria isolated from commercial yoghurt samples | |
| KR101796500B1 (en) | Lactobacillus Pentosus GFC03 and Fermented Whey Manufactured Using Thereof | |
| CN111991280A (en) | Anti-allergy bean fermentation extract, product and preparation method thereof | |
| Hawaz et al. | Characterization of lactic acid bacteria from camel milk and their technological properties to use as a starter culture | |
| KR102310336B1 (en) | Lactobacillus plantarum q1 strain having complex digestive productivity and antibacterial activity and uses thereof | |
| CN114073659B (en) | Preparation and application of fermentation product of lactobacillus paracasei from distillers' grains of chicken feet wine in Pan-Mahala region | |
| Arihara et al. | Antimicrobial activity of Enterococcus faecalis against Listeria monocytogenes | |
| CN110452849A (en) | A kind of probiotic lactobacillus plantarum | |
| Gulahmadov et al. | Isolation and characterization of bacteriocin-like inhibitory substances from lactic acid bacteria isolated from Azerbaijan cheeses | |
| Batjargal | Probiotic Properties of Lactic Acid Bacteria Isolated from Mongolian Fermented Mare’s Milk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210101 |